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Pesquisa : A10.615.284.473 [Categoria DeCS]
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[PMID]:29249255
[Au] Autor:Sun Q; Chen Z; He P; Li Y; Ding X; Huang Y; Gu H; Ni X
[Ad] Endereço:Department of Physiology, Second Military Medical University, Shanghai, China; Department of Gynecology and Obstetrics, Changhai Hospital, Shanghai, China.
[Ti] Título:Reduced Expression of Hydrogen Sulfide-Generating Enzymes Down-Regulates 15-Hydroxyprostaglandin Dehydrogenase in Chorion during Term and Preterm Labor.
[So] Source:Am J Pathol;188(1):63-71, 2018 01.
[Is] ISSN:1525-2191
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chorionic NAD-dependent 15-hydroxyprostaglandin dehydrogenase (PGDH) plays a pivotal role in controlling the amount of prostaglandins in the uterus and has been implicated in the process of labor. Prior studies identified hydrogen sulfide-generating enzymes cystathionine-ß-synthetase (CBS) and cystathionine-γ-lyase (CSE) in fetal membranes. We investigated whether hydrogen sulfide is involved in the regulation of PGDH expression in the chorion during labor. The chorionic tissues were obtained from pregnant women at preterm in labor and at term in labor or not in labor at term. Levels of CSE and CBS and hydrogen sulfide production rate were down-regulated in term in labor and preterm in labor groups compared with not in labor at term group. The CBS level correlated to PGDH expression in the chorion. Hydrogen sulfide donor NaHS and precursor l-cysteine dose-dependently stimulated PGDH expression and activity in cultured chorionic trophoblasts. The effect of l-cysteine was blocked by CBS inhibitor and CBS siRNA but not by CSE inhibitor and CSE siRNA. Hydrogen sulfide treatment suppressed miR-26b and miR-199a expression in chorionic trophoblasts. miR-26b and miR-199a mimics blocked hydrogen sulfide upregulation of PGDH expression. Our results indicate that hydrogen sulfide plays pivotal roles in maintenance of PGDH expression in the chorion during human pregnancy. Reduced expression of hydrogen sulfide-generating enzymes contributes to an increased amount of prostaglandins in the uterus during labor.
[Mh] Termos MeSH primário: Córion/enzimologia
Cistationina beta-Sintase/metabolismo
Cistationina gama-Liase/metabolismo
Hidroxiprostaglandina Desidrogenases/metabolismo
Trabalho de Parto Prematuro/metabolismo
Nascimento a Termo/metabolismo
[Mh] Termos MeSH secundário: Cistationina gama-Liase/genética
Regulação para Baixo
Feminino
Seres Humanos
Sulfeto de Hidrogênio/metabolismo
Hidroxiprostaglandina Desidrogenases/genética
Trabalho de Parto Prematuro/genética
Gravidez
Nascimento a Termo/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
EC 1.1.1.- (Hydroxyprostaglandin Dehydrogenases); EC 1.1.1.141 (15-hydroxyprostaglandin dehydrogenase); EC 4.2.1.22 (Cystathionine beta-Synthase); EC 4.4.1.1 (Cystathionine gamma-Lyase); YY9FVM7NSN (Hydrogen Sulfide)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180131
[Lr] Data última revisão:
180131
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE


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[PMID]:29205585
[Au] Autor:Klaritsch P
[Ad] Endereço:Department of Obstetrics and Gynecology, Medical University Graz, Auenbruggerplatz 14, A-8036, Graz, Austria.
[Ti] Título:Re: Influence of chorionicity and gestational age at single fetal loss on risk of preterm birth in twin pregnancy: analysis of STORK multiple pregnancy cohort. F. D'Antonio, B. Thilaganathan, T. Dias and A. Khalil, on behalf of the Southwest Thames Obstetric Research Collaborative (STORK). Ultrasound Obstet Gynecol 2017; 50: 723-727.
[So] Source:Ultrasound Obstet Gynecol;50(6):681-682, 2017 12.
[Is] ISSN:1469-0705
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Idade Gestacional
Gravidez de Gêmeos
[Mh] Termos MeSH secundário: Córion
Feminino
Seres Humanos
Gravidez
Nascimento Prematuro
Cuidado Pré-Natal
[Pt] Tipo de publicação:JOURNAL ARTICLE; COMMENT
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180123
[Lr] Data última revisão:
180123
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171206
[St] Status:MEDLINE
[do] DOI:10.1002/uog.18939


  3 / 3646 MEDLINE  
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[PMID]:28797129
[Au] Autor:Go YY; Kim SE; Cho GJ; Chae SW; Song JJ
[Ad] Endereço:Department of Otorhinolaryngology-Head and Neck Surgery, Korea University College of Medicine, Seoul, Korea.
[Ti] Título:Differential effects of amnion and chorion membrane extracts on osteoblast-like cells due to the different growth factor composition of the extracts.
[So] Source:PLoS One;12(8):e0182716, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Human amniotic membrane extracts contain numerous growth factors and bioactive substances. However, osteogenic effects of amnion and chorion membrane extracts (AME and CME, respectively) on osteoblasts are unclear. In this study, we explored the ability of AME and CME to promote the osteogenic differentiation of osteoblast-like MG-63 cells. MG-63 cells were cultured in osteogenic induction medium (OIM) with or without exogenous AME and CME. CME enhanced the osteogenic differentiation of MG-63 cells compared with AME, as indicated by increased mineralization; alkaline phosphatase activity; and mRNA expression of osteogenic marker genes encoding integrin-binding sialoprotein (IBSP), RUNX2, OSTERIX, and osteocalcin (OCN). Interestingly, AME and CME contained different combinations of osteogenesis-related growth factors, including basic fibroblast growth factor (bFGF), transforming growth factor beta-1 (TGFß-1), and epidermal growth factor (EGF), which differentially regulated the osteogenic differentiation of MG-63 cells. bFGF and TGFß-1 present in CME positively regulated the osteogenic differentiation of MG-63 cells, whereas EGF present in AME negatively regulated the differentiation of MG-63 cells. Moreover, exogenous treatment of EGF antagonized CME-induced mineralization of extracellular matrix on MG-63 cells. We compared the osteogenic efficacy of CME with that of BMP2, bFGF, and TGFß-1 alone or their combinations. We observed that CME greatly enhanced osteogenesis by providing a conductive environment for the differentiation of MG-63 cells. Together, our results indicated that human AME and CME exerted differential effects on osteogenesis because of the presence of different compositions of growth factors. In addition, our results highlighted a new possible strategy of using CME as a biocompatible therapeutic material for bone regeneration.
[Mh] Termos MeSH primário: Peptídeos e Proteínas de Sinalização Intercelular/fisiologia
Osteoblastos/fisiologia
[Mh] Termos MeSH secundário: Âmnio/química
Benzodioxóis/farmacologia
Linhagem Celular
Proliferação Celular
Forma Celular
Córion/química
Seres Humanos
Imidazóis/farmacologia
Peptídeos e Proteínas de Sinalização Intercelular/isolamento & purificação
Osteogênese
Piridinas/farmacologia
Pirróis/farmacologia
Extratos de Tecidos/isolamento & purificação
Extratos de Tecidos/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (2-(5-benzo(1,3)dioxol-5-yl-2-tert-butyl-3H-imidazol-4-yl)-6-methylpyridine hydrochloride); 0 (Benzodioxoles); 0 (Imidazoles); 0 (Intercellular Signaling Peptides and Proteins); 0 (Pyridines); 0 (Pyrroles); 0 (SU 5402); 0 (Tissue Extracts)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170811
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182716


  4 / 3646 MEDLINE  
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[PMID]:28699878
[Au] Autor:Poskin A; Martinelle L; Van der Stede Y; Saegerman C; Cay B; De Regge N
[Ad] Endereço:1​CODA-CERVA, Operational Directorate Viral Diseases, Groeselenberg 99, 1180 Brussels, Belgium.
[Ti] Título:Genetically stable infectious Schmallenberg virus persists in foetal envelopes of pregnant ewes.
[So] Source:J Gen Virol;98(7):1630-1635, 2017 Jul.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Schmallenberg virus (SBV) is a recently emerged vector-borne virus, inducing congenital defects in bovines, ovines and caprines. Here we have shown that infectious SBV is capable of persisting until the moment of birth in the foetal envelopes of ewes infected with SBV-infectious serum at day 45 (1/5 positive) and 60 (4/6 positive) of gestation. This persistence of at least 100 days is a new aspect of the SBV pathogenesis that could help to explain how SBV overwinters the cold season in temperate climate zones. Furthermore, sequencing of the M segment shows that the persisting virus in the foetal envelopes is genetically stable since only a few mutations compared to the inoculum were found. This supports the hypothesis that persisting virus could start the infection of new hosts. Finally, neutralization tests showed that infectious SBV present in the foetal envelopes at birth can be neutralized by the humoral immunity present in the infected ewes.
[Mh] Termos MeSH primário: Infecções por Bunyaviridae/virologia
Córion/virologia
Orthobunyavirus/genética
Placenta/virologia
RNA Viral/genética
Doenças dos Ovinos/virologia
Ovinos/virologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Antivirais/imunologia
Sequência de Bases
Infecções por Bunyaviridae/imunologia
Feminino
Orthobunyavirus/imunologia
Orthobunyavirus/isolamento & purificação
Gravidez
Análise de Sequência de RNA
Doenças dos Ovinos/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (RNA, Viral)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170804
[Lr] Data última revisão:
170804
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170713
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000841


  5 / 3646 MEDLINE  
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[PMID]:28591337
[Au] Autor:Souza MA; de Lourdes Brizot M; Biancolin SE; Schultz R; de Carvalho MHB; Francisco RPV; Zugaib M
[Ad] Endereço:Departamento de Ginecologia e Obstetricia, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, BR.
[Ti] Título:Placental weight and birth weight to placental weight ratio in monochorionic and dichorionic growth-restricted and non-growth-restricted twins.
[So] Source:Clinics (Sao Paulo);72(5):265-271, 2017 May.
[Is] ISSN:1980-5322
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE:: The aim of the present study was to compare the placental weight and birth weight/placental weight ratio for intrauterine growth-restricted and non-intrauterine growth-restricted monochorionic and dichorionic twins. METHODS:: This was a retrospective analysis of placentas from twin pregnancies. Placental weight and the birth weight/placental weight ratio were compared in intrauterine growth-restricted and non-intrauterine growth-restricted monochorionic and dichorionic twins. The association between cord insertion type and placental lesions in intrauterine growth-restricted and non-intrauterine growth-restricted monochorionic and dichorionic twins was also investigated. RESULTS:: A total of 105 monochorionic (intrauterine growth restriction=40; non-intrauterine growth restriction=65) and 219 dichorionic (intrauterine growth restriction=57; non-intrauterine growth restriction=162) placentas were analyzed. A significantly lower placental weight was observed in intrauterine growth-restricted monochorionic (p=0.022) and dichorionic (p<0.001) twins compared to non-intrauterine growth-restricted twins. There was no difference in the birth weight/placental weight ratio between the intrauterine growth restriction and non-intrauterine growth restriction groups for either monochorionic (p=0.36) or dichorionic (p=0.68) twins. Placental weight and the birth weight/placental weight ratio were not associated with cord insertion type or with placental lesions. CONCLUSION:: Low placental weight, and consequently reduced functional mass, appears to be involved in fetal growth restriction in monochorionic and dichorionic twins. The mechanism by which low placental weight influences the birth weight/placental weight ratio in intrauterine growth-restricted monochorionic and dichorionic twins needs to be determined in larger prospective studies.
[Mh] Termos MeSH primário: Peso ao Nascer/fisiologia
Córion
Desenvolvimento Fetal/fisiologia
Retardo do Crescimento Fetal/fisiopatologia
Placenta/anatomia & histologia
Gravidez de Gêmeos/fisiologia
[Mh] Termos MeSH secundário: Adulto
Córion/fisiologia
Feminino
Idade Gestacional
Seres Humanos
Tamanho do Órgão
Placenta/patologia
Placenta/fisiopatologia
Gravidez
Valores de Referência
Estudos Retrospectivos
Estatísticas não Paramétricas
Fatores de Tempo
Gêmeos Dizigóticos
Gêmeos Monozigóticos
Adulto Jovem
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170608
[St] Status:MEDLINE


  6 / 3646 MEDLINE  
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[PMID]:28414752
[Au] Autor:Huang W; Itayama M; Arai F; Furukawa KS; Ushida T; Kawahara T
[Ad] Endereço:Department of Biological Functions Engineering, Kyushu Institute of Technology, Wakamatsu-ku, Kitakyushu, Japan.
[Ti] Título:An angiogenesis platform using a cubic artificial eggshell with patterned blood vessels on chicken chorioallantoic membrane.
[So] Source:PLoS One;12(4):e0175595, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The chorioallantoic membrane (CAM) containing tiny blood vessels is an alternative to large animals for studies involving angiogenesis and tissue engineering. However, there is no technique to design the direction of growing blood vessels on the CAM at the microscale level for tissue engineering experiments. Here, a methodology is provided to direct blood vessel formation on the surface of a three-dimensional egg yolk using a cubic artificial eggshell with six functionalized membranes. A structure on the lateral side of the eggshell containing a straight channel and an interlinked chamber was designed, and the direction and formation area of blood vessels with blood flow was artfully defined by channels with widths of 70-2000 µm, without sharply reducing embryo viability. The relationship between the size of interlinked chamber and the induction of blood vessels was investigated to establish a theory of design. Role of negative and positive pressure in the induction of CAM with blood vessels was investigated, and air pressure change in the culture chamber was measured to demonstrate the mechanism for blood vessel induction. Histological evaluation showed that components of CAM including chorionic membrane and blood vessels were induced into the channels. Based on our design theory, blood vessels were induced into arrayed channels, and channel-specific injection and screening were realized, which demonstrated proposed applications. The platform with position- and space-controlled blood vessels is therefore a powerful tool for biomedical research, which may afford exciting applications in studies involved in local stimulation of blood vessel networks and those necessary to establish a living system with blood flow from a beating heart.
[Mh] Termos MeSH primário: Vasos Sanguíneos/fisiologia
Galinhas/fisiologia
Membrana Corioalantoide/irrigação sanguínea
Membrana Corioalantoide/fisiologia
Casca de Ovo/fisiologia
Neovascularização Fisiológica/fisiologia
[Mh] Termos MeSH secundário: Animais
Embrião de Galinha
Córion/fisiologia
Morfogênese/fisiologia
Fluxo Sanguíneo Regional/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170505
[Lr] Data última revisão:
170505
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170418
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0175595


  7 / 3646 MEDLINE  
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[PMID]:28350838
[Au] Autor:Verbruggen SW; Oyen ML; Phillips AT; Nowlan NC
[Ad] Endereço:Department of Bioengineering, Imperial College London, London, United Kingdom.
[Ti] Título:Function and failure of the fetal membrane: Modelling the mechanics of the chorion and amnion.
[So] Source:PLoS One;12(3):e0171588, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The fetal membrane surrounds the fetus during pregnancy and is a thin tissue composed of two layers, the chorion and the amnion. While rupture of this membrane normally occurs at term, preterm rupture can result in increased risk of fetal mortality and morbidity, as well as danger of infection in the mother. Although structural changes have been observed in the membrane in such cases, the mechanical behaviour of the human fetal membrane in vivo remains poorly understood and is challenging to investigate experimentally. Therefore, the objective of this study was to develop simplified finite element models to investigate the mechanical behaviour and rupture of the fetal membrane, particularly its constituent layers, under various physiological conditions. It was found that modelling the chorion and amnion as a single layer predicts remarkably different behaviour compared with a more anatomically-accurate bilayer, significantly underestimating stress in the amnion and under-predicting the risk of membrane rupture. Additionally, reductions in chorion-amnion interface lubrication and chorion thickness (reported in cases of preterm rupture) both resulted in increased membrane stress. Interestingly, the inclusion of a weak zone in the fetal membrane that has been observed to develop overlying the cervix would likely cause it to fail at term, during labour. Finally, these findings support the theory that the amnion is the dominant structural component of the fetal membrane and is required to maintain its integrity. The results provide a novel insight into the mechanical effect of structural changes in the chorion and amnion, in cases of both normal and preterm rupture.
[Mh] Termos MeSH primário: Âmnio/fisiologia
Colo do Útero/fisiologia
Córion/fisiologia
Análise de Elementos Finitos
Útero/fisiologia
[Mh] Termos MeSH secundário: Algoritmos
Âmnio/fisiopatologia
Colo do Útero/fisiopatologia
Córion/fisiopatologia
Feminino
Ruptura Prematura de Membranas Fetais/fisiopatologia
Idade Gestacional
Seres Humanos
Trabalho de Parto
Gravidez
Estresse Mecânico
Nascimento a Termo
Útero/fisiopatologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170329
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0171588


  8 / 3646 MEDLINE  
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[PMID]:28343898
[Au] Autor:Araújo AB; Salton GD; Furlan JM; Schneider N; Angeli MH; Laureano ÁM; Silla L; Passos EP; Paz AH
[Ad] Endereço:Cryobiology Unit and Umbilical Cord Blood Bank, Hemotherapy Service, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil; Federal University of Rio Grande do Sul, Porto Alegre, Brazil. Electronic address: anelise_araujo@yahoo.com.br.
[Ti] Título:Comparison of human mesenchymal stromal cells from four neonatal tissues: Amniotic membrane, chorionic membrane, placental decidua and umbilical cord.
[So] Source:Cytotherapy;19(5):577-585, 2017 May.
[Is] ISSN:1477-2566
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Mesenchymal stromal cells (MSCs) are being investigated as a potential alternative for cellular therapy. This study was designed to compare the biological characteristics of MSCs isolated from amniotic membrane (A-MSCs), chorionic membrane (C-MSCs), placental decidua (D-MSCs) and umbilical cord (UC-MSCs) to ascertain whether any one of these sources is superior to the others for cellular therapy purposes. METHODS: MSCs were isolated from amniotic membrane, chorionic membrane, umbilical cord and placental decidua. Immunophenotype, differentiation ability, cell size, cell complexity, polarity index and growth kinetics of MSCs isolated from these four sources were analyzed. RESULTS: MSCs were successfully isolated from all four sources. Surface marker profile and differentiation ability were consistent with human MSCs. C-MSCs in suspension were the smallest cells, whereas UC-MSCs presented the greatest length and least width. A-MSCs had the lowest polarity index and UC-MSCs, as more elongated cells, the highest. C-MSCs, D-MSCs and UC-MSCs exhibited similar growth capacity until passage 8 (P8); C-MSCs presented better lifespan, whereas insignificant proliferation was observed in A-MSCs. DISCUSSION: Neonatal and maternal tissues can serve as sources of multipotent stem cells. Some characteristics of MSCs obtained from four neonatal tissues were compared and differences were observed. Amniotic membrane was the least useful source of MSCs, whereas chorionic membrane and umbilical cord were considered good options for future use in cell therapy because of the known advantages of immature cells.
[Mh] Termos MeSH primário: Âmnio/citologia
Córion/citologia
Decídua/citologia
Células Mesenquimais Estromais/citologia
Cordão Umbilical/citologia
[Mh] Termos MeSH secundário: Diferenciação Celular
Proliferação Celular
Forma Celular
Células Cultivadas
Feminino
Seres Humanos
Imunofenotipagem
Recém-Nascido
Cinética
Gravidez
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170328
[St] Status:MEDLINE


  9 / 3646 MEDLINE  
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[PMID]:28255007
[Au] Autor:Muench MO; Kapidzic M; Gormley M; Gutierrez AG; Ponder KL; Fomin ME; Beyer AI; Stolp H; Qi Z; Fisher SJ; Bárcena A
[Ad] Endereço:Blood Systems Research Institute, San Francisco, CA 94118, USA.
[Ti] Título:The human chorion contains definitive hematopoietic stem cells from the fifteenth week of gestation.
[So] Source:Development;144(8):1399-1411, 2017 04 15.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We examined the contribution of the fetal membranes, amnion and chorion, to human embryonic and fetal hematopoiesis. A population of cells displaying a hematopoietic progenitor phenotype (CD34 CD45 ) of fetal origin was present in the chorion at all gestational ages, associated with stromal cells or near blood vessels, but was absent in the amnion. Prior to 15 weeks of gestation, these cells lacked hematopoietic engraftment potential. Differences in the chemokine receptor and ß1 integrin expression profiles of progenitors between the first and second trimesters suggest that these cells had gestationally regulated responses to homing signals and/or adhesion mechanisms that influenced their ability to colonize the stem cell niche. Definitive hematopoietic stem cells, capable of multilineage and long-term reconstitution when transplanted in immunodeficient mice, were present in the chorion from 15-24 weeks gestation, but were absent at term. The second trimester cells also engrafted secondary recipients in serial transplantation experiments. Thus, the human chorion contains functionally mature hematopoietic stem cells at mid-gestation.
[Mh] Termos MeSH primário: Córion/citologia
Células-Tronco Hematopoéticas/citologia
[Mh] Termos MeSH secundário: Animais
Antígenos CD/metabolismo
Moléculas de Adesão Celular/metabolismo
Contagem de Células
Linhagem da Célula
Córion/transplante
Vilosidades Coriônicas/metabolismo
Colagenases/metabolismo
Feminino
Feto/citologia
Seres Humanos
Integrina beta1/metabolismo
Camundongos SCID
Fenótipo
Gravidez
Trimestres da Gravidez/metabolismo
Receptores de Quimiocinas/metabolismo
Tripsina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antigens, CD); 0 (Cell Adhesion Molecules); 0 (Integrin beta1); 0 (Receptors, Chemokine); EC 3.4.21.4 (Trypsin); EC 3.4.24.- (Collagenases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170304
[St] Status:MEDLINE
[do] DOI:10.1242/dev.138438


  10 / 3646 MEDLINE  
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[PMID]:28244611
[Au] Autor:Vorgia E; Zaragkoulias A; Peraki I; Mavrothalassitis G
[Ad] Endereço:Medical School, University of Crete, Heraklion, Crete, Greece.
[Ti] Título:Suppression of Fgf2 by ETS2 repressor factor (ERF) is required for chorionic trophoblast differentiation.
[So] Source:Mol Reprod Dev;84(4):286-295, 2017 Apr.
[Is] ISSN:1098-2795
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:ETS2 repressor factor (ERF) is a ubiquitous transcriptional repressor regulated by Extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylation. Homozygous deletion of Erf in mice blocks chorionic trophoblast differentiation, resulting in the failure of chorioallantoic fusion and subsequent embryo death. Fibroblast growth factor (FGF) signaling is important for proper trophoblast stem cell (TSC) differentiation and development of the hemochorial placenta. Lack of Fgf2 promotes TSC differentiation, while FGF4 or FGF2 is required for murine TSC maintenance. Here, we show that low in vivo Fgf2 mRNA abundance occurs in patches of placental chorion cells and ex vivo in TSCs. This expression is repressed via direct interaction of ERF with the Fgf2 transcription unit is increased in the absence of ERF, and is decreased in the presence of an ERF mutant resistant to ERK phosphorylation. Thus, FGF2 inhibition by ERF appears to be necessary for proper chorionic TSC differentiation, and may account for the block of chorionic trophoblast differentiation in Erf-knockout animals. The differentiation of ERF-overexpressing TSC lines also suggests that ERF may have an FGF2-independent effect during the commitment towards syncytiotrophoblasts.
[Mh] Termos MeSH primário: Diferenciação Celular/fisiologia
Córion/metabolismo
Fator 2 de Crescimento de Fibroblastos/metabolismo
Sistema de Sinalização das MAP Quinases/fisiologia
Proteína Proto-Oncogênica c-ets-2/metabolismo
Trofoblastos/metabolismo
[Mh] Termos MeSH secundário: Animais
Córion/citologia
Fator 2 de Crescimento de Fibroblastos/genética
Fator 4 de Crescimento de Fibroblastos/genética
Fator 4 de Crescimento de Fibroblastos/metabolismo
Camundongos
Camundongos Knockout
Proteína Quinase 3 Ativada por Mitógeno/metabolismo
Fosforilação/fisiologia
Trofoblastos/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ets2 protein, mouse); 0 (Fgf4 protein, mouse); 0 (Fibroblast Growth Factor 4); 0 (Proto-Oncogene Protein c-ets-2); 103107-01-3 (Fibroblast Growth Factor 2); EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170301
[St] Status:MEDLINE
[do] DOI:10.1002/mrd.22780



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