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Pesquisa : A10.615.284.473.200 [Categoria DeCS]
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[PMID]:29308857
[Au] Autor:Nizyaeva NV; Sukhacheva TV; Kulikova GV; Nagovitsyna MN; Kan NE; Baev OR; Pavlovich SV; Serov RA; Shchegolev AI; Poltavtseva RA
[Ti] Título:Morphological Features of Mesenhymal Stroma Cells of Chorionic Villi.
[So] Source:Vestn Ross Akad Med Nauk;72(1):76-83, 2017.
[Is] ISSN:0869-6047
[Cp] País de publicação:Russia (Federation)
[La] Idioma:eng
[Ab] Resumo:Background: Nowadays autologous mesenchymal placental stromal cells (MSCs) may use to treat for various diseases both of the mother and the child. Stroma of the placenta villi is appropriated origin for cell culture isolation. Aim: of the study was to evaluate the possibility for selection and use of placental tissue for mesenchymal stromal cells. Materials and methods: The present study was based on 45 placental samples of women aged 27−38 yy. who underwent surgical delivery at 36−40 weeks of gestation. 30 of these women have been enrolled in the basic group including children with congenital abnormalities (CA). The comparison group consisted of 15 patients with physiological pregnancy. We performed histological examination (with hematoxylin and eosin staining), immunohistochemical examination (with use monoclonal antibodies CD90 (1:25; Abcam, UK), СD105 (1:500; Abcam, UK), CD44 (1:25; Dako), СD73 (1:200, Abcam, UK), and electron microscopy (by microscope Philips/FEI Corporation, Eindhoven, Holland). Eclipse 80i microscope (Nikon Corporation, Japan) was used to examine the immunohistochemical reactions as a brown staining. The evaluation of the intensity of reaction was conducted by NIS-Elements Advanced Research 3.2 program (Czech Republic). Student's t-test and analysis of variance were used to compare the mean values. Differences were considered statistically significant at p<0.05. Results: Interstitial cells of the stroma of the villi with CA had fibroblastic differentiation as revealed degenerative changes of the cells. The histologic examination with hematoxylin and eosin staining revealed significant fibrosis of the stroma of the placenta villi in CA group (p<0,01). Immunohistochemical study of stem and intermediate chorionic villi revealed no significant differences in staining of CD44+, СD90+, СD73+, and CD105+ cells if compared to the control group (p>0.05). Although CD105 expression was significantly lower in the CA group (0.058±0.0049) than in the control group (0.088±0.0039) (p<0.05). However, electron microscopy detected the villi interstitial stromal cells with fibroblastic differentiation in CA group. Conclusions: Thus, it is necessary to exclude placenta with obstetrical history, somatic, and congenital pathology of the mother and the child when selecting the placental cell culture. Moreover, choosing a sample the morphological structure of the placenta should be taken into consideration. However, congenital malformations of the fetus, pathology of the mother cultivate mesenchymal stromal cells of placentas is inappropriate and should be taken advantage of the donor cells.
[Mh] Termos MeSH primário: Vilosidades Coriônicas
Anormalidades Congênitas/diagnóstico
Seleção do Doador/métodos
Células Mesenquimais Estromais
Placenta/patologia
[Mh] Termos MeSH secundário: Adulto
Técnicas de Cultura de Células/métodos
Vilosidades Coriônicas/diagnóstico por imagem
Vilosidades Coriônicas/patologia
Amostra da Vilosidade Coriônica/métodos
Feminino
Fibrose
Seres Humanos
Imuno-Histoquímica
Transplante de Células-Tronco Mesenquimais/métodos
Células Mesenquimais Estromais/patologia
Microscopia Eletrônica/métodos
Gravidez
Estatística como Assunto
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180109
[St] Status:MEDLINE
[do] DOI:10.15690/vramn767


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[PMID]:28456990
[Au] Autor:Verma J; Bijarnia-Mahay S; Verma IC
[Ad] Endereço:Institute of Medical Genetics and Genomics, Sir Ganga Ram Hospital, New Delhi, India.
[Ti] Título:Prenatal Diagnosis of Lysosomal Storage Disorders Using Chorionic Villi.
[So] Source:Methods Mol Biol;1594:265-291, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Prenatal enzymatic diagnosis for an array of lysosomal storage disorders (LSDs) can be performed accurately, provided that a confirmed diagnosis by biochemical/molecular study in the index case is available and a strict defined protocol, specific to each individual disorder is followed. The present chapter describes the protocols for reliable and accurate prenatal enzymatic diagnoses by fluorometric and spectrophotometric methods of lysosomal storage disorders: Gaucher, Fabry, Pompe, Niemann Pick A/B, Tay Sach, Sandhoff, GM1, Mucoplysaccharidoses, Wolman, Krabbe, Metachromatic leukodystrophy, and Batten diseases using uncultured chorionic villi samples. The biological reference intervals for enzyme levels in normal and affected fetuses are given for interpretation of prenatal results. It is imperative to establish normal reference interval in each laboratory to take into account the local environment, technical variations, and different ethnicities. Besides, enzyme activity in the fetus should be represented as percentage of the mean activity of enzyme of normal fetuses. The pitfalls and challenges in prenatal diagnosis as well as technical problems in performing enzyme assays are also discussed to help the reader in standardization and performing the assays for correct diagnosis.
[Mh] Termos MeSH primário: Vilosidades Coriônicas/enzimologia
Vilosidades Coriônicas/metabolismo
Doenças por Armazenamento dos Lisossomos/diagnóstico
Doenças por Armazenamento dos Lisossomos/enzimologia
Diagnóstico Pré-Natal/métodos
[Mh] Termos MeSH secundário: Feminino
Feto/enzimologia
Feto/metabolismo
Seres Humanos
Gravidez
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170501
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-4939-6934-0_18


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[PMID]:28912064
[Au] Autor:Zhu Y; Li B; Wu T; Ye L; Zeng Y; Zhang Y
[Ad] Endereço:School of Life Sciences, Tsinghua University, Beijing, China; Key Lab in Healthy Science and Technology, Division of Life Science, Graduate School at Shenzhen, Tsinghua University, Shenzhen, China.
[Ti] Título:Cell cycle and histone modification genes were decreased in placenta tissue from unexplained early miscarriage.
[So] Source:Gene;636:17-22, 2017 Dec 15.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Genetic defect is a major cause of early miscarriage, but still in many cases the etiology are not fully understood. Recent studies have shown that dysregulation of genes in placenta tissue are participated in the pathogenesis of unexplained early miscarriage. The aim of our study is to explore mRNA expression profile in placental chorionic villi and to reveal the underlying mechanism of unexplained early miscarriage. Chorionic villous were isolated and extracted from early miscarriage (n=3) and control pregnancy (n=3) placenta with normal chromosome karyotype using MLPA assay, and then mRNA expression profiles were determined by microarray. For verification the reproducibility of the microarray, three up-regulated genes and six down-regulated genes were chosen and examined by real-time PCR (n=30). A total of 81 genes were up-regulated and 231 genes were down-regulated when compared to the control group, and the differences were reached statistically significances (P<0.05). After Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses, we found that almost down-regulation genes are associated with cell cycle and histone modification, and these genes are participated in several important physiological processes, such as cell proliferation, nuclear division, chromatic assembly, DNA packing and modification. These results indicated that cell cycle and histone modification genes, and related signaling pathway maybe contribute to the genesis and development of unexplained early miscarriage. Further studies and validations are necessary to elucidate the exact roles of these genes in miscarriage pathogenesis, which can develop tools for early detection and management.
[Mh] Termos MeSH primário: Aborto Espontâneo/genética
Genes cdc
Código das Histonas/genética
Placenta/metabolismo
[Mh] Termos MeSH secundário: Aborto Espontâneo/metabolismo
Vilosidades Coriônicas/metabolismo
Feminino
Regulação da Expressão Gênica
Ontologia Genética
Seres Humanos
Gravidez
Mapas de Interação de Proteínas
RNA Mensageiro/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Messenger)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170916
[St] Status:MEDLINE


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[PMID]:28662500
[Au] Autor:Soler A; Morales C; Mademont-Soler I; Margarit E; Borrell A; Borobio V; Muñoz M; Sánchez A
[Ad] Endereço:Servei de Bioquímica i Genètica Molecular, Barcelona, Spain.
[Ti] Título:Overview of Chromosome Abnormalities in First Trimester Miscarriages: A Series of 1,011 Consecutive Chorionic Villi Sample Karyotypes.
[So] Source:Cytogenet Genome Res;152(2):81-89, 2017.
[Is] ISSN:1424-859X
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:In order to contribute to the knowledge of type and frequency of chromosome abnormalities in early pregnancy losses, we analyzed the cytogenetic results from a large series of first trimester miscarriages, using a diagnostic approach with a high success rate and no maternal contamination. A total of 1,119 consecutive chorionic villi samples were obtained before evacuation, and karyotypes were prepared after short-term culture (STC). In 603 samples, a long-term culture (LTC) was also performed. The overall and individual frequencies of the different types of chromosome abnormalities were established, including placental mosaicisms, and their relationship with maternal age and gestational weeks was assessed. An abnormal karyotype was detected in 70.3% of the samples. Single autosomal trisomy was the most frequent abnormality (64.6% of the abnormal cases), followed by triploidy (13.1%) and monosomy X (10.4%). Chromosome rearrangements were found in 5.2%, combined abnormalities in 8.9%, and placental mosaicism in 3.5% of the cases with STC and LTC performed. Individual trisomies behaved differently with respect to maternal age and intrauterine survival. Due to the combination of STC and LTC, our study offers reliable information on the incidence and type of chromosome abnormalities and placental mosaicism in miscarriages and contributes to define the cytogenetic implication in their etiology.
[Mh] Termos MeSH primário: Aborto Espontâneo/genética
Vilosidades Coriônicas/metabolismo
Aberrações Cromossômicas
Cariótipo
Cariotipagem/métodos
Primeiro Trimestre da Gravidez/genética
[Mh] Termos MeSH secundário: Feminino
Rearranjo Gênico/genética
Idade Gestacional
Seres Humanos
Idade Materna
Mosaicismo
Placenta/patologia
Ploidias
Gravidez
Cromossomos Sexuais/genética
Trissomia/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170630
[St] Status:MEDLINE
[do] DOI:10.1159/000477707


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[PMID]:28549829
[Au] Autor:Ridano ME; Racca AC; Flores-Martin JB; Fretes R; Bandeira CL; Reyna L; Bevilacqua E; Genti-Raimondi S; Panzetta-Dutari GM
[Ad] Endereço:Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), Universidad Nacional de Córdoba,CONICET, Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Córdoba, Argentina.
[Ti] Título:Impact of chlorpyrifos on human villous trophoblasts and chorionic villi.
[So] Source:Toxicol Appl Pharmacol;329:26-39, 2017 Aug 15.
[Is] ISSN:1096-0333
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Placental barrier regulates maternal-fetal interchange protecting the baby from damage caused by substances found in the uterine environment or circulating in the vascular system. Organophosphate (OP) pesticides are a paramount group of environmental pollutants used in intensive agriculture for protection against diseases and pests. While many studies have reported an increased risk of pregnancy alterations in pregnant women exposed to OPs, few have analyzed the effects caused by these pesticides in the placenta. Herein, we evaluated the effects of chlorpyrifos (CPF), one of the most widely used OP insecticides, on human placenta using in vitro and ex vivo exposure models. Villous cytotrophoblast cells isolated from normal human term placentas maintained their cell viability, differentiated into syncytiotrophoblast-like structures, and increased the expression of ß-hCG, ABCG2, and P-gp in the presence of CPF at concentrations of 10 to 100µM. The same doses of CPF induced marked changes in chorionic villi samples. Indeed, CPF exposure increased stroma cell apoptosis, altered villi matrix composition, basement membrane thickness, and trophoblastic layer integrity. Histomorphological and ultrastructural alterations are compatible with those found in placentas where maternal-placenta injury is chronic and able to impair the placental barrier function and nutrient transport from mother to the fetus. Our study shows that placental ex vivo exposure to CPF produces tissue alterations and suggest that human placenta is a potential target of CPF toxicity. In addition, it highlights the importance of using different models to assess the effects of a toxic on human placenta.
[Mh] Termos MeSH primário: Clorpirifos/toxicidade
Inibidores da Colinesterase/toxicidade
Vilosidades Coriônicas/efeitos dos fármacos
Inseticidas/toxicidade
Trofoblastos/efeitos dos fármacos
[Mh] Termos MeSH secundário: Subfamília B de Transportador de Cassetes de Ligação de ATP/metabolismo
Membro 2 da Subfamília G de Transportadores de Cassetes de Ligação de ATP/metabolismo
Apoptose/efeitos dos fármacos
Membrana Basal/efeitos dos fármacos
Membrana Basal/ultraestrutura
Bioensaio
Diferenciação Celular/efeitos dos fármacos
Células Cultivadas
Gonadotropina Coriônica Humana Subunidade beta/metabolismo
Vilosidades Coriônicas/metabolismo
Vilosidades Coriônicas/ultraestrutura
Relação Dose-Resposta a Droga
Feminino
Seres Humanos
Proteínas de Neoplasias/metabolismo
Gravidez
Reprodutibilidade dos Testes
Medição de Risco
Células Estromais/efeitos dos fármacos
Células Estromais/ultraestrutura
Fatores de Tempo
Técnicas de Cultura de Tecidos
Testes de Toxicidade/métodos
Trofoblastos/metabolismo
Trofoblastos/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ABCB1 protein, human); 0 (ABCG2 protein, human); 0 (ATP Binding Cassette Transporter, Sub-Family B); 0 (ATP Binding Cassette Transporter, Sub-Family G, Member 2); 0 (Cholinesterase Inhibitors); 0 (Chorionic Gonadotropin, beta Subunit, Human); 0 (Insecticides); 0 (Neoplasm Proteins); JCS58I644W (Chlorpyrifos)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170528
[St] Status:MEDLINE


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[PMID]:28547981
[Au] Autor:Stanek J
[Ti] Título:Placental infectious villitis versus villitis of unknown etiology.
[So] Source:Pol J Pathol;68(1):55-65, 2017.
[Is] ISSN:1233-9687
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:To assess the incidence, diagnosis, pathogenesis, and clinical and placental associations of congenital cytomegalovirus infection, 34 cases thereof diagnosed by placental/fetal or neonatal workup (group 1), and 494 placentas with villitis of unknown etiology (group 2) were extracted from a 6083-case placental database. 28 clinical and 47 placental phenotypes were compared between the two groups by Yates 2 or ANOVA using the Bonferroni correction. 26 group 1 cases did and 8 did not feature placental villitis, but all cases were positive as shown by immunohistochemistry and/or in situ hybridization. Only 5 differences were statistically significant (p Bonferroni < 0.0056): gestational age 29.8 ±6.5 vs. 35.5 ±4.9 weeks, perinatal mortality 67.6 vs. 16.2%, nonmacerated stillbirth 20.6 vs. 3.0%, macerated stillbirth 38.2 vs. 9.3%, and diffuse villous fibrosis 44.1 vs. 12.5%, between group 1 and group 2, respectively. The absence of significant differences in placental phenotypes between group 1 and group 2 other than the histological pattern of villitis indicates that not the cytomegalovirus villitis but the direct viral cytopathogenic effect on fetal organs makes the difference in the dire clinical outcome in the former. As about a third of cytomegalovirus infections show no villitis, the combination of the clinical picture and placental patterns creates the best chance to detect congenital cytomegalovirus infection.
[Mh] Termos MeSH primário: Vilosidades Coriônicas/patologia
Infecções por Citomegalovirus/patologia
Doenças Placentárias/patologia
Doenças Placentárias/virologia
Complicações Infecciosas na Gravidez/patologia
[Mh] Termos MeSH secundário: Adulto
Feminino
Seres Humanos
Imuno-Histoquímica
Gravidez
Complicações Infecciosas na Gravidez/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170527
[St] Status:MEDLINE


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[PMID]:28412999
[Au] Autor:Parveen S; Panicker MM; Gupta PK
[Ad] Endereço:School of Regenerative Medicine, Manipal University, GKVK post, Bellary Road, Bengaluru 560065, Karnataka, India. Electronic address: shagufta.parveen@manipal.edu.
[Ti] Título:Generation of an induced pluripotent stem cell line from chorionic villi of a Turner syndrome spontaneous abortion.
[So] Source:Stem Cell Res;19:12-16, 2017 Mar.
[Is] ISSN:1876-7753
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A major cause of spontaneous abortions is chromosomal abnormality of foetal cells. We report the generation of an induced pluripotent stem cell line from the fibroblasts isolated from chorionic villi of an early spontaneously aborted foetus with Turner syndrome. The Turner syndrome villus induced pluripotent stem cell line is transgene free, retains the original XO karyotype, expresses pluripotency markers and undergoes trilineage differentiation. This pluripotent stem cell model of Turner syndrome should serve as a tool to study the developmental abnormalities of foetus and placenta that lead to early embryo lethality and profound symptoms like infertility in 45 XO survivors.
[Mh] Termos MeSH primário: Células-Tronco Pluripotentes Induzidas/citologia
Síndrome de Turner/patologia
[Mh] Termos MeSH secundário: Aborto Espontâneo
Biomarcadores/metabolismo
Linhagem Celular
Reprogramação Celular
Vilosidades Coriônicas/metabolismo
Feminino
Seres Humanos
Células-Tronco Pluripotentes Induzidas/metabolismo
Cariótipo
Gravidez
Fatores de Transcrição/genética
Fatores de Transcrição/metabolismo
Síndrome de Turner/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Transcription Factors)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170504
[Lr] Data última revisão:
170504
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170418
[St] Status:MEDLINE


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[PMID]:28408374
[Au] Autor:Chui A; Gunatillake T; Brennecke SP; Ignjatovic V; Monagle PT; Whitelock JM; van Zanten DE; Eijsink J; Wang Y; Deane J; Borg AJ; Stevenson J; Erwich JJ; Said JM; Murthi P
[Ad] Endereço:From the Department of Obstetrics and Gynaecology, The University of Melbourne, Parkville, Victoria, Australia (A.C., T.G., S.P.B., P.M.); Sunshine Hospital, St Albans, Victoria, Australia (A.C., T.G., S.P.B., P.M.); Department of Maternal-Fetal Medicine Pregnancy Research Centre, The Royal Women's
[Ti] Título:Expression of Biglycan in First Trimester Chorionic Villous Sampling Placental Samples and Altered Function in Telomerase-Immortalized Microvascular Endothelial Cells.
[So] Source:Arterioscler Thromb Vasc Biol;37(6):1168-1179, 2017 Jun.
[Is] ISSN:1524-4636
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Biglycan (BGN) has reduced expression in placentae from pregnancies complicated by fetal growth restriction (FGR). We used first trimester placental samples from pregnancies with later small for gestational age (SGA) infants as a surrogate for FGR. The functional consequences of reduced BGN and the downstream targets of were determined. Furthermore, the expression of targets was validated in primary placental endothelial cells isolated from FGR or control pregnancies. APPROACH AND RESULTS: expression was determined using real-time polymerase chain reaction in placental tissues collected during chorionic villous sampling performed at 10 to 12 weeks' gestation from pregnancies that had known clinical outcomes, including SGA. Short-interference RNA reduced expression in telomerase-immortalized microvascular endothelial cells, and the effect on proliferation, angiogenesis, and thrombin generation was determined. An angiogenesis array identified downstream targets of BGN, and their expression in control and FGR primary placental endothelial cells was validated using real-time polymerase chain reaction. Reduced expression was observed in SGA placental tissues. reduction decreased network formation of telomerase-immortalized microvascular endothelial cells but did not affect thrombin generation or cellular proliferation. The array identified target genes, which were further validated: angiopoetin 4 ( ), platelet-derived growth factor receptor α ( ), tumor necrosis factor superfamily member 15 ( ), angiogenin ( ), serpin family C member 1 ( ), angiopoietin 2 ( ), and CXC motif chemokine 12 ( ) in telomerase-immortalized microvascular endothelial cells and primary placental endothelial cells obtained from control and FGR pregnancies. CONCLUSIONS: This study reports a temporal relationship between altered placental expression and subsequent development of SGA. Reduction of in vascular endothelial cells leads to disrupted network formation and alterations in the expression of genes involved in angiogenesis. Therefore, differential expression of these may contribute to aberrant angiogenesis in SGA pregnancies.
[Mh] Termos MeSH primário: Biglicano/metabolismo
Vilosidades Coriônicas/irrigação sanguínea
Vilosidades Coriônicas/metabolismo
Células Endoteliais/metabolismo
Retardo do Crescimento Fetal/metabolismo
Microvasos/metabolismo
Neovascularização Fisiológica
Primeiro Trimestre da Gravidez/metabolismo
Telomerase/metabolismo
[Mh] Termos MeSH secundário: Animais
Biglicano/genética
Estudos de Casos e Controles
Linhagem Celular
Amostra da Vilosidade Coriônica
Feminino
Retardo do Crescimento Fetal/genética
Retardo do Crescimento Fetal/fisiopatologia
Perfilação da Expressão Gênica
Regulação da Expressão Gênica no Desenvolvimento
Seres Humanos
Recém-Nascido
Recém-Nascido Pequeno para a Idade Gestacional
Masculino
Camundongos Endogâmicos C57BL
Neovascularização Fisiológica/genética
Gravidez
Primeiro Trimestre da Gravidez/genética
Interferência de RNA
Transdução de Sinais
Telomerase/genética
Trombina/metabolismo
Fatores de Tempo
Técnicas de Cultura de Tecidos
Transfecção
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (BGN protein, human); 0 (Biglycan); EC 2.7.7.49 (Telomerase); EC 3.4.21.5 (Thrombin)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170620
[Lr] Data última revisão:
170620
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170415
[St] Status:MEDLINE
[do] DOI:10.1161/ATVBAHA.117.309422


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[PMID]:28396992
[Au] Autor:Schwartz DA
[Ad] Endereço:Department of Pathology, Medical College of Georgia, Augusta University, Augusta, Georgia. davidalanschwartz@gmail.com.
[Ti] Título:Viral infection, proliferation, and hyperplasia of Hofbauer cells and absence of inflammation characterize the placental pathology of fetuses with congenital Zika virus infection.
[So] Source:Arch Gynecol Obstet;295(6):1361-1368, 2017 Jun.
[Is] ISSN:1432-0711
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Attention is increasingly focused on the potential mechanism(s) for Zika virus infection to be transmitted from an infected mother to her fetus. This communication addresses current evidence for the role of the placenta in vertical transmission of the Zika virus. METHODS: Placentas from second and third trimester fetuses with confirmed intrauterine Zika virus infection were examined with routine staining to determine the spectrum of pathologic changes. In addition, immunohistochemical staining for macrophages and nuclear proliferation antigens was performed. Viral localization was identified using RNA hybridization. These observations were combined with the recent published results of placental pathology to increase the strength of the pathology data. Results were correlated with published data from experimental studies of Zika virus infection in placental cells and chorionic villous explants. RESULTS: Placentas from fetuses with congenital Zika virus infection are concordant in not having viral-induced placental inflammation. Special stains reveal proliferation and prominent hyperplasia of placental stromal macrophages, termed Hofbauer cells, in the chorionic villi of infected placentas. Zika virus infection is present in Hofbauer cells from second and third trimester placentas. Experimental studies and placentae from infected fetuses reveal that the spectrum of placental cell types infected with the Zika virus is broader during the first trimester than later in gestation. CONCLUSIONS: Inflammatory abnormalities of the placenta are not a component of vertical transmission of the Zika virus. The major placental response in second and third trimester transplacental Zika virus infection is proliferation and hyperplasia of Hofbauer cells, which also demonstrate viral infection.
[Mh] Termos MeSH primário: Hiperplasia/patologia
Placenta/patologia
Complicações Infecciosas na Gravidez/virologia
Infecção pelo Zika virus/complicações
[Mh] Termos MeSH secundário: Proliferação Celular
Vilosidades Coriônicas/patologia
Feminino
Feto/patologia
Seres Humanos
Recém-Nascido
Transmissão Vertical de Doença Infecciosa
Inflamação/patologia
Macrófagos/patologia
Placenta/virologia
Gravidez
Complicações Infecciosas na Gravidez/patologia
Primeiro Trimestre da Gravidez
Terceiro Trimestre da Gravidez
Infecção pelo Zika virus/transmissão
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170412
[St] Status:MEDLINE
[do] DOI:10.1007/s00404-017-4361-5


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[PMID]:28350871
[Au] Autor:Truong G; Guanzon D; Kinhal V; Elfeky O; Lai A; Longo S; Nuzhat Z; Palma C; Scholz-Romero K; Menon R; Mol BW; Rice GE; Salomon C
[Ad] Endereço:Exosome Biology Laboratory, Centre for Clinical Diagnostics, University of Queensland Centre for Clinical Research, Royal Brisbane and Women's Hospital, The University of Queensland, Brisbane, Queensland, Australia.
[Ti] Título:Oxygen tension regulates the miRNA profile and bioactivity of exosomes released from extravillous trophoblast cells - Liquid biopsies for monitoring complications of pregnancy.
[So] Source:PLoS One;12(3):e0174514, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Our understanding of how cells communicate has undergone a paradigm shift since the recent recognition of the role of exosomes in intercellular signaling. In this study, we investigated whether oxygen tension alters the exosome release and miRNA profile from extravillous trophoblast (EVT) cells, modifying their bioactivity on endothelial cells (EC). Furthermore, we have established the exosomal miRNA profile at early gestation in women who develop pre-eclampsia (PE) and spontaneous preterm birth (SPTB). HTR-8/SVneo cells were used as an EVT model. The effect of oxygen tension (i.e. 8% and 1% oxygen) on exosome release was quantified using nanocrystals (Qdot®) coupled to CD63 by fluorescence NTA. A real-time, live-cell imaging system (Incucyte™) was used to establish the effect of exosomes on EC. Plasma samples were obtained at early gestation (<18 weeks) and classified according to pregnancy outcomes. An Illumina TrueSeq Small RNA kit was used to construct a small RNA library from exosomal RNA obtained from EVT and plasma samples. The number of exosomes was significantly higher in EVT cultured under 1% compared to 8% oxygen. In total, 741 miRNA were identified in exosomes from EVT. Bioinformatic analysis revealed that these miRNA were associated with cell migration and cytokine production. Interestingly, exosomes isolated from EVT cultured at 8% oxygen increased EC migration, whilst exosomes cultured at 1% oxygen decreased EC migration. These changes were inversely proportional to TNF-α released from EC. Finally, we have identified a set of unique miRNAs in exosomes from EVT cultured at 1% oxygen and exosomes isolated from the circulation of mothers at early gestation, who later developed PE and SPTB. We suggest that aberrant exosomal signalling by placental cells is a common aetiological factor in pregnancy complications characterised by incomplete SpA remodeling and is therefore a clinically relevant biomarker of pregnancy complications.
[Mh] Termos MeSH primário: Exossomos/secreção
Perfilação da Expressão Gênica/métodos
MicroRNAs/genética
Oxigênio/metabolismo
Trofoblastos/metabolismo
[Mh] Termos MeSH secundário: Adulto
Artérias/metabolismo
Biópsia/métodos
Western Blotting
Movimento Celular/genética
Células Cultivadas
Vilosidades Coriônicas/metabolismo
Análise por Conglomerados
Exossomos/genética
Exossomos/ultraestrutura
Feminino
Células Endoteliais da Veia Umbilical Humana/citologia
Células Endoteliais da Veia Umbilical Humana/metabolismo
Seres Humanos
MicroRNAs/sangue
Microscopia Eletrônica de Transmissão
Oxigênio/farmacologia
Gravidez
Complicações na Gravidez/sangue
Complicações na Gravidez/genética
Complicações na Gravidez/metabolismo
Trofoblastos/citologia
Trofoblastos/efeitos dos fármacos
Útero/irrigação sanguínea
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MicroRNAs); S88TT14065 (Oxygen)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170831
[Lr] Data última revisão:
170831
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170329
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0174514



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