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[PMID]:29431926
[Au] Autor:Kalaev VN; Nechaeva MS
[Ti] Título:[The study of the frequency of buccal epithelium cells with nuclear in athletes in dependence on the place got in the competition].
[So] Source:Gig Sanit;95(10):992-7, 2016.
[Is] ISSN:0016-9900
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Cytogenetic homeostasis ofathletes involved in hand-to-hand armyfighting, depending on the outcome of the fight and the level of aggressiveness has been studied. Status of the genetic apparatus in athletes was evaluated in buccal epithelium with the use of micronucleus test, which is widely usedfor the determination of the influence of various factors on the genetic stability of the organism. Psychological testing of athletes was executed with the use of Spielberger's State-Trait Anger Expression Inventory scale, Bass-Darky questionnaire for the diagnostics of aggression and hostile reactions, Eysenck Personality Questionnaire test for the evaluation ofpsychic conditions and method of U.P. Ilyin and P.A. Kovalev for the identification of the propensity to conflictness and aggressiveness (positive and negative) as the personal characteristics. The place taken by the athlete on the competition was shown to have an impact on the frequency of buccal epithelium cells with nuclear abnormalities (micronuclei, perinuclear vacuoles, notches, protrusions "tongue" and "broken egg"). Losers have nuclear aberrations more than winners. At that in athletes with higher aggressiveness and its attendant psychological characteristics the influence of the outcome of the competition on the number of cells with aberrations core is more pronounced.
[Mh] Termos MeSH primário: Agressão
Desempenho Atlético
Artes Marciais
Testes para Micronúcleos/métodos
Mucosa Bucal/patologia
[Mh] Termos MeSH secundário: Adolescente
Agressão/fisiologia
Agressão/psicologia
Atletas/psicologia
Desempenho Atlético/fisiologia
Desempenho Atlético/psicologia
Análise Citogenética/métodos
Seres Humanos
Masculino
Artes Marciais/fisiologia
Artes Marciais/psicologia
Testes Psicológicos
Estatística como Assunto
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180213
[St] Status:MEDLINE


  2 / 24387 MEDLINE  
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[PMID]:29431345
[Au] Autor:Ingel FI; Yurchenko VV; Krivtsova EK; Urtseva NA; Legostaeva TB
[Ti] Título:[Buccal micronucleus cytome assay in complex genetic-hygienic study of preschool children].
[So] Source:Gig Sanit;95(10):969-73, 2016.
[Is] ISSN:0016-9900
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:There are presented results of the cytome analysis of epithelium of buccal mucosa in 167 children aged of 5-7 years, permanently residing in the city of Magnitogorsk and visiting municipal kindergartens. Frequencies of the main indices of genotoxic and toxic effects were: cells with micronuclei - 0.29±0.04 %, with protrusions ofnuclei - 1.47±0.17 %, with double nuclei -1.96±0.13 %, with two nuclei - 3,28±0,16 %, with perinuclear vacuole - 9.66±0.86 %, with pycnosis of nucleus - 4.60±0.31 per 1000 cells, with karyorrhexis - 0.86±0.15per 1000 cells, with lysis of nucleus - 54.34±3.90 per 1000 cells, the condensed chromatin cells - 10.59±1.35 per 1000 cells. Most of these indices didn't differ from the same detected earlier in buccal epithelial cells from children of the same age in Moscow, except cells with perinuclear vacuole, which frequency in Moscow cohort was twice higher. Frequencies of cells with these anomalies were associated with the content of 19 out of 326 revealed components of contamination of the total-winter snow samples, taken on the territories of the kindergartens, which were visited by examined children. There was shown lack of differences from basic values of main indices previously establishedfor the children of the same age in Moscow, with the exception of cells with perinuclear vacuole, the rate of which in Moscow was twice higher. There were revealed confounding factors associated with test indices: gender of the child, the annual number of acute respiratory diseases, health group, income per one person in family, alcohol intake by parents.
[Mh] Termos MeSH primário: Exposição Ambiental
Testes para Micronúcleos
Mucosa Bucal/patologia
Poluição da Água
[Mh] Termos MeSH secundário: Criança
Pré-Escolar
Exposição Ambiental/efeitos adversos
Exposição Ambiental/análise
Feminino
Disparidades nos Níveis de Saúde
Seres Humanos
Masculino
Testes para Micronúcleos/métodos
Testes para Micronúcleos/estatística & dados numéricos
Medição de Risco/métodos
Medição de Risco/estatística & dados numéricos
Fatores de Risco
Federação Russa/epidemiologia
Neve
Poluição da Água/efeitos adversos
Poluição da Água/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180213
[St] Status:MEDLINE


  3 / 24387 MEDLINE  
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[PMID]:29458673
[Au] Autor:Morse DJ; Wilson MJ; Wei X; Lewis MAO; Bradshaw DJ; Murdoch C; Williams DW
[Ad] Endereço:1​Oral and Biomedical Sciences, School of Dentistry, Cardiff University, Cardiff, UK.
[Ti] Título:Denture-associated biofilm infection in three-dimensional oral mucosal tissue models.
[So] Source:J Med Microbiol;67(3):364-375, 2018 Mar.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: In vitro analyses of virulence, pathogenicity and associated host cell responses are important components in the study of biofilm infections. The Candida-related infection, denture-associated oral candidosis, affects up to 60 % of denture wearers and manifests as inflammation of palatal tissues contacting the denture-fitting surface. Commercially available three-dimensional tissue models can be used to study infection, but their use is limited for many academic research institutions, primarily because of the substantial purchase costs. The aim of this study was to develop and evaluate the use of in vitro tissue models to assess infections by biofilms on acrylic surfaces through tissue damage and Candida albicans virulence gene expression. METHODOLOGY: In vitro models were compared against commercially available tissue equivalents (keratinocyte-only, SkinEthic; full-thickness, MatTek Corporation). An in vitro keratinocyte-only tissue was produced using a cancer-derived cell line, TR146, and a full-thickness model incorporating primary fibroblasts and immortalised normal oral keratinocytes was also generated. The in vitro full-thickness tissues incorporated keratinocytes and fibroblasts, and have potential for future further development and analysis. RESULTS: Following polymicrobial infection with biofilms on acrylic surfaces, both in-house developed models were shown to provide equivalent results to the SkinEthic and MatTek models in terms of tissue damage: a significant (P<0.05) increase in LDH activity for mixed species biofilms compared to uninfected control, and no significant difference (P>0.05) in the expression of most C. albicans virulence genes when comparing tissue models of the same type. CONCLUSION: Our results confirm the feasibility and suitability of using these alternative in vitro tissue models for such analyses.
[Mh] Termos MeSH primário: Biofilmes/crescimento & desenvolvimento
Candidíase Bucal/microbiologia
Dentaduras/microbiologia
Interações Hospedeiro-Patógeno
Mucosa Bucal/microbiologia
[Mh] Termos MeSH secundário: Candida albicans/genética
Candida albicans/patogenicidade
Candida albicans/fisiologia
Linhagem Celular
Coinfecção/microbiologia
Fibroblastos/microbiologia
Seres Humanos
Queratinócitos/microbiologia
Polimetil Metacrilato
Estomatite sob Prótese
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9011-14-7 (Polymethyl Methacrylate)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000677


  4 / 24387 MEDLINE  
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Lemos, Tadeu
Texto completo SciELO Brasil
[PMID]:29267663
[Au] Autor:Sordi MB; Massochin RC; Camargo AR; Lemos T; Munhoz EA
[Ad] Endereço:Universidade Federal de Santa Catarina - UFSC, Health Science Centre, Department of Stomatology, Florianópolis, SC, Brazil.
[Ti] Título:Oral health assessment for users of marijuana and cocaine/crack substances.
[So] Source:Braz Oral Res;31:e102, 2017 Dec 18.
[Is] ISSN:1807-3107
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:The objective of this study was to assess the oral health status of users of illicit drugs such as marijuana and cocaine/crack and compare it with individuals not using these chemical substances. Questionnaires were applied to 35 illicit drugs users to gather information on demographic status, general health, and use of drugs. Then, a clinical assessment of the oral health condition was performed to collect data on decayed, missing and filled teeth (DMFT) index, salivary flow rate (SFR), and mucosal lesions. The control group was composed of 35 non-illicit drug users. In the experimental group, 91.43% were males, 80% were smokers, and 42.85% were alcoholics. Cocaine was the most common drug used (77.15%), followed by marijuana (68.6%), and crack (51.4%). The average DMFT index was 9.8 and the SFR was reduced in 60% of subjects. Mucosal alterations were detected, but no potentially malignant disorders or oral cancer were diagnosed. Compared to control group, significantly higher values for gender (40%, p = 0.0001), smoking (22.86%) and heavy drinking (5.7%) habits (p = 0.0001), SFR (31.4%; p = 0.0308), and oral lesions (p = 0.0488) were found for the experimental group, although significantly higher values were found in the control group for DMFT index (p = 0.0148). It can be concluded that the use of illicit drugs contributed to an increased prevalence of oral mucosa lesions. In addition, a decline on SFR and a reduced DMFT index was observed for illicit drug users.
[Mh] Termos MeSH primário: Transtornos Relacionados ao Uso de Cocaína/complicações
Abuso de Maconha/complicações
Doenças da Boca/induzido quimicamente
Mucosa Bucal/efeitos dos fármacos
Saúde Bucal/estatística & dados numéricos
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Alcoolismo/complicações
Alcoolismo/epidemiologia
Estudos de Casos e Controles
Transtornos Relacionados ao Uso de Cocaína/epidemiologia
Estudos Transversais
Índice CPO
Feminino
Seres Humanos
Masculino
Abuso de Maconha/epidemiologia
Meia-Idade
Doenças da Boca/epidemiologia
Fatores de Risco
Salivação/efeitos dos fármacos
Taxa Secretória/efeitos dos fármacos
Distribuição por Sexo
Fumar/efeitos adversos
Fumar/epidemiologia
Fatores Socioeconômicos
Inquéritos e Questionários
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE


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[PMID]:28749039
[Au] Autor:Mukhtar BMB; Spilotros M; Malde S; Greenwell TJ
[Ad] Endereço:Department of Urology, University College London Hospital at Westmoreland Street, London, UK.
[Ti] Título:Ventral-onlay buccal mucosa graft substitution urethroplasty for urethral stricture in women.
[So] Source:BJU Int;120(5):710-716, 2017 11.
[Is] ISSN:1464-410X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To present our outcomes of ventral-onlay buccal mucosa graft (BMG) substitution urethroplasty in treating female urethral stricture (FUS). PATIENTS AND METHODS: We conducted a review of a prospectively collected database of 22 consecutive women (median [range] age 50 [34-72] years) with urethral stricture who underwent ventral onlay BMG substitution urethroplasty after June 2012 and who had a minimum follow-up of 6 months (median 21.5, range 6-51 months). Data were analysed for stricture recurrence, change in median maximum urinary flow rate (Q ) and median post-void residual urine volume (PVR). Statistical analysis was performed using the Wilcoxon signed rank test, Student's t-test and the Mann-Whitney U-test. RESULTS: Freedom from stricture recurrence was achieved in 21/22 (95.5%) women. The median (range) Q significantly improved, increasing from 7 (3.5-11) to 18 (5-37) mL/s (P <0.05). The median (range) PVR was significantly reduced from 100 (0-300) to 15 (0-150) mL (P < 0.05). Short- and longer-term complication rates were low. One woman developed mild de novo stress urinary incontinence, which settled with conservative management by 6 months. CONCLUSIONS: Early and medium-term results indicate that ventral onlay BMG substitution urethroplasty is an excellent treatment for FUS that can avoid the need for the repeat procedures regularly required after traditional endoscopic management.
[Mh] Termos MeSH primário: Mucosa Bucal/cirurgia
Transplantes/cirurgia
Transplantes/transplante
Uretra/cirurgia
Estreitamento Uretral/cirurgia
Procedimentos Cirúrgicos Urológicos/métodos
[Mh] Termos MeSH secundário: Adulto
Idoso
Feminino
Seres Humanos
Meia-Idade
Complicações Pós-Operatórias
Estudos Prospectivos
Procedimentos Cirúrgicos Urológicos/efeitos adversos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1111/bju.13970


  6 / 24387 MEDLINE  
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[PMID]:29430903
[Au] Autor:Sycheva LP; Budarina OV; Sabirova ZF; Akhaltseva LV; Rosolovsky AP
[Ti] Título:[Cytogenetic status of children in the hygienic assessment of the air pollution by odorous substances].
[So] Source:Gig Sanit;95(8):765-8, 2016.
[Is] ISSN:0016-9900
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:For the first time the impact of odorous emissions from the enterprise for the production of chewing gum on the cytogenetic status of children, residing in the distance of 0,9 and 6 km, was studied. Specific weak mint and fruity odour in the air in the placement of the enterprise may be related to the presence of menthol, menthone, pinene, limonene, ethyl acetate and other compounds containing in the emissions. There were no differences in cytogenetic status of children in both groups (by frequency of micronuclei, protrusions, binucleated cells, apoptosis).
[Mh] Termos MeSH primário: Poluentes Atmosféricos
Mucosa Bucal/patologia
Odorantes
[Mh] Termos MeSH secundário: Poluentes Atmosféricos/efeitos adversos
Poluentes Atmosféricos/análise
Poluentes Atmosféricos/classificação
Pré-Escolar
Análise Citogenética/métodos
Análise Citogenética/estatística & dados numéricos
Exposição Ambiental/efeitos adversos
Exposição Ambiental/prevenção & controle
Feminino
Seres Humanos
Masculino
Indústria Manufatureira/normas
Odorantes/análise
Odorantes/prevenção & controle
Estatística como Assunto
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Air Pollutants)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180213
[St] Status:MEDLINE


  7 / 24387 MEDLINE  
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[PMID]:29425005
[Au] Autor:Novikova IV; Agafonov GV; Korotkikh EA; Kalaev VN; Nechaeva MS; Mal'tseva OY
[Ti] Título:[Evaluation of antimutagenic properties of powdered malt and polymalt extracts with the use of micronucleus test].
[So] Source:Gig Sanit;95(7):669-75, 2016.
[Is] ISSN:0016-9900
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:There were performed studies of the occurrence of cells with pathologies in the buccal epithelium of volunteers who consume drinks based on mixtures ofpowdered malt and polymalt extracts of buckwheat, peas, corn and barley. There was shown their impact on the stability of the genetic material of examined cases. There was established activation of apoptosis, which leads to the elimination of cells with cytogenetic deteriorations. Poliymalt extracts possess protective properties, contribute to the suppression ofprocesses offormation of cells with genetic disorders (micronuclei (from 4.38 ± 0.67 %%, up to 2.53 ± 0.39 %% after intake), protrusions (from 1,98 ± 0,42 %%, up 0,85 ± 0,25 %% after intake), incisures (from 3.34 ± 0.44 %%, up 2.17 ± 0.35 %% after intake), two cores (from 1.63 ± 0.26 %%, up 0.65 ± 0.21 %% after intake) and rid the body of aberrant cells, as evidenced by the increase in the number of cells with karyolysis (up to 5.98 ± 0,91 %%, up 9.55 ± 1.74 %% after intake), karyopyknosis (from 10.71 ± 0.90 %%, up to 11.97 ± 0.85 %% after intake) and perinuclear vacuoles (from 9.24 ± 1.63 %%, up to 12.94 ± 2.57 %% after intake). In women, anti-mutagenic effects ofpolymalt extracts are more pronounced than in men. Antimutagenic effects of extracts can be explained by the properties of contained in them B vitamins and sulfur-containing amino acids (cysteine and methionine).
[Mh] Termos MeSH primário: Antimutagênicos/farmacologia
Grãos Comestíveis
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Antioxidantes/farmacologia
Feminino
Seres Humanos
Masculino
Testes para Micronúcleos/métodos
Mucosa Bucal/patologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antimutagenic Agents); 0 (Antioxidants); 0 (Plant Extracts)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180210
[St] Status:MEDLINE


  8 / 24387 MEDLINE  
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[PMID]:29424501
[Au] Autor:Reutova NV; Dreeva FR; Reutova TV; Shevchenko AV; Dudarov ZI
[Ti] Título:[Effect of recultivated wastes of mining factories on children residing in the district of their location].
[So] Source:Gig Sanit;95(6):572-6, 2016.
[Is] ISSN:0016-9900
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Wastes and tailing ponds of Tyrnyauz tungsten and molybdenum factory are the main sources of heavy metal incoming into environment in Kabardino-Balkarian Republic. The factory was closed more than 10 years ago and the recultivation of it's tailing ponds, where accumulated hundreds of millions tones of wastes, was completed. The aim of this investigation was an assessment of their possible influence on children residing in the vicinity of these tailing ponds (village Bylym). Village Verhny Baksan located about 30 km upstream of the valley of the Baksan River was chosen as reference (pure) locality. As a results of the performed investigations we revealed that in drinking water of Bylym and Verhny Baksan concentrations of molybdenum were 2.10±0.42 pg/l and 0.31±0.15 pg/l correspondingly, which is remarkably lower than maximum permitted concentrations. The concentrations of Mo, Cu and Pb in children's hair in both villages were practically the same, which indicates to the absence of their accumulation in human organism. But the quantity of cells with cytogenetic disorders in buccal epithelial cells in children from Bylym was 4.1 times higher in comparison with the corresponding index of uncontaminated area. The obtained data demonstrate that genotoxic effect of remedied tailing ponds retains.
[Mh] Termos MeSH primário: Exposição Ambiental
Cabelo/química
Metais Pesados
Micronúcleos com Defeito Cromossômico/induzido quimicamente
Mineração
Poluentes do Solo
Instalações de Eliminação de Resíduos/normas
Poluentes Químicos da Água
[Mh] Termos MeSH secundário: Criança
Exposição Ambiental/efeitos adversos
Exposição Ambiental/análise
Exposição Ambiental/prevenção & controle
Monitoramento Ambiental/métodos
Monitoramento Ambiental/estatística & dados numéricos
Feminino
Seres Humanos
Masculino
Metais Pesados/análise
Metais Pesados/normas
Mineração/métodos
Mineração/normas
Mucosa Bucal/patologia
Federação Russa/epidemiologia
Poluentes do Solo/análise
Poluentes do Solo/normas
Poluentes Químicos da Água/análise
Poluentes Químicos da Água/normas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Metals, Heavy); 0 (Soil Pollutants); 0 (Water Pollutants, Chemical)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180210
[St] Status:MEDLINE


  9 / 24387 MEDLINE  
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[PMID]:29412553
[Au] Autor:Ladnova GG; Istomin AV; Kurochitskaya MG; Silyutina VV
[Ti] Título:[Cytogenetic indices of buccal epithelium in schoolchildren residing in territories with different levels of the air pollution].
[So] Source:Gig Sanit;95(5):428-31, 2016.
[Is] ISSN:0016-9900
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:In the article there are contained the results of the study of ambient air of the regional center, urban-type settlement, and buccal epithelium in 87 schoolchildren aged of 11-13 years. Schoolchildren residing in the regional center were shown to be more susceptible to the exposure to such toxicants as carbon monoxide, nitrogen oxide, fluorhydric acid, chlorine and its compounds, sulfur dioxide, formaldehyde contained in the ambient air. In this group of students if compared with students who live in the village, there were revealed more pronounced cytogenetic deteriorations in oral mucosal epitheliocytes: cells with micronuclei, different forms of protrusion of the core, dual-core cell mitosis, kariokynesis, apoptotic bodies significantly more common occur, that can speak about both not only of cytogenetic instability, but and the degree of environmental genotoxicity, in particular, ambient air. There was noted the tendency to increase the level of cytogenetic damage and destruction of the nucleus in the group of girls in comparison with boys.
[Mh] Termos MeSH primário: Substâncias Perigosas
Mucosa Bucal/patologia
[Mh] Termos MeSH secundário: Adolescente
Poluentes Atmosféricos/efeitos adversos
Poluentes Atmosféricos/análise
Criança
Análise Citogenética/métodos
Exposição Ambiental/prevenção & controle
Monitoramento Ambiental/métodos
Monitoramento Ambiental/estatística & dados numéricos
Feminino
Substâncias Perigosas/efeitos adversos
Substâncias Perigosas/análise
Seres Humanos
Masculino
Determinação de Necessidades de Cuidados de Saúde
Poluentes Radioativos/efeitos adversos
Poluentes Radioativos/análise
Federação Russa/epidemiologia
Serviços de Saúde Escolar/estatística & dados numéricos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Air Pollutants); 0 (Hazardous Substances); 0 (Radioactive Pollutants)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180208
[St] Status:MEDLINE


  10 / 24387 MEDLINE  
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[PMID]:29254288
[Au] Autor:Dyszkiewicz-Konwinska M; Bryja A; Jopek K; Budna J; Khozmi R; Jeseta M; Bukowska D; Antosik P; Bruska M; Nowicki M; Zabel M; Kempisty B
[Ad] Endereço:Department of Biomaterials and Experimental Dentistry, Poznan University of Medical Sciences, Poznan, Poland.
[Ti] Título:Expression of genes responsible for cell morphogenesis involved in differentiation in porcine buccal pouch mucosal cells during long-term primary culture and real-time proliferation in vitro.
[So] Source:J Biol Regul Homeost Agents;31(4):855-864, 2017 Oct-Dec.
[Is] ISSN:0393-974X
[Cp] País de publicação:Italy
[La] Idioma:eng
[Ab] Resumo:Recently, using experimental animal model, we demonstrated that porcine buccal pouch mucosal cells reflect increased proliferation capability during primary cultivation in vitro. Although the histological structure and morphogenesis in oral cavity is well recognized, the molecular mechanisms which regulate this process still need further investigation. This study was aimed to analyze the molecular marker expression profile involved in morphogenesis and differentiation capacity of porcine buccal pouch mucosal cells during their long-term primary cultivation in vitro. The experiment was performed on buccal pouch mucosal cells isolated from 80 pubertal crossbred Landrace gilts. After collection, the cells were treated enzymatically and transferred into a primary in vitro culture (IVC) system and cultured for 30 days. The cells were collected for RNA isolation after 7, 15 and 30 days of IVC and were checked for their real-time proliferative status using the RTCA system. We found an increased expression of FN1 and SOX9 genes when calculated against ACTB after 7, and 30 days of IVC, (P less than 0.01, P less than 0.001, respectively). The CXCL12 mRNA was down-regulated after 7, 15 and 30 days of IVC, but not statistically significant. Similar expression profile was observed when calculated against HPRT, however, DAB2 was found to be higher expressed at day 15 of IVC, (P less than 0.05). The cell index measured during real-time cell proliferation was substantially increased between 96 h and 147h of IVC and reached the log phase. Since FN1 and SOX9 revealed significant increase of expression after long-term culture in vitro, it is suggested that expression of these differentiation and stemness genes is accompanied by cell proliferation. Moreover, FN1 and SOX9 might be recognized as new markers of buccal pouch mucosal cell proliferation and differentiation in pigs in in vitro primary culture model.
[Mh] Termos MeSH primário: Células Epiteliais/metabolismo
Regulação da Expressão Gênica no Desenvolvimento
Lipocalina-2/genética
Morfogênese/genética
Mucosa Bucal/metabolismo
Fatores de Transcrição SOX9/genética
[Mh] Termos MeSH secundário: Animais
Diferenciação Celular
Proliferação Celular
Quimiocina CXCL12/genética
Quimiocina CXCL12/metabolismo
Células Epiteliais/citologia
Feminino
Perfilação da Expressão Gênica
Hipoxantina Fosforribosiltransferase/genética
Hipoxantina Fosforribosiltransferase/metabolismo
Lipocalina-2/metabolismo
Mucosa Bucal/citologia
Mucosa Bucal/crescimento & desenvolvimento
Cultura Primária de Células
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Fatores de Transcrição SOX9/metabolismo
Transdução de Sinais
Suínos
Proteínas Supressoras de Tumor/genética
Proteínas Supressoras de Tumor/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chemokine CXCL12); 0 (Lipocalin-2); 0 (RNA, Messenger); 0 (SOX9 Transcription Factor); 0 (Tumor Suppressor Proteins); EC 2.4.2.8 (Hypoxanthine Phosphoribosyltransferase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE



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