Base de dados : MEDLINE
Pesquisa : A10.690.552 [Categoria DeCS]
Referências encontradas : 370 [refinar]
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[PMID]:29304082
[Au] Autor:Gadek KE; Wang H; Hall MN; Sungello M; Libby A; MacLaskey D; Eckel RH; Olwin BB
[Ad] Endereço:Department of Molecular, Cellular and Developmental Biology, University of Colorado Boulder, Boulder, Colorado United States of America.
[Ti] Título:Striated muscle gene therapy for the treatment of lipoprotein lipase deficiency.
[So] Source:PLoS One;13(1):e0190963, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Excessive circulating triglycerides due to reduction or loss of lipoprotein lipase activity contribute to hypertriglyceridemia and increased risk for pancreatitis. The only gene therapy treatment for lipoprotein lipase deficiency decreases pancreatitis but minimally reduces hypertriglyceridemia. Synthesized in multiple tissues including striated muscle and adipose tissue, lipoprotein lipase is trafficked to blood vessel endothelial cells where it is anchored at the plasma membrane and hydrolyzes triglycerides into free fatty acids. We conditionally knocked out lipoprotein lipase in differentiated striated muscle tissue lowering striated muscle lipoprotein lipase activity causing hypertriglyceridemia. We then crossed lipoprotein lipase striated muscle knockout mice with mice possessing a conditional avian retroviral receptor gene and injected mice with either a human lipoprotein lipase retrovirus or an mCherry control retrovirus. Post-heparin plasma lipoprotein lipase activity increased for three weeks following human lipoprotein lipase retroviral infection compared to mCherry infected mice. Human lipoprotein lipase infected mice had significantly lower blood triglycerides compared to mCherry controls and were comparable to wild-type blood triglyceride levels. Thus, targeted delivery of human lipoprotein lipase into striated muscle tissue identifies a potential therapeutic target for lipoprotein lipase deficiency.
[Mh] Termos MeSH primário: Terapia Genética
Lipase Lipoproteica/genética
Músculo Estriado/patologia
[Mh] Termos MeSH secundário: Animais
Vetores Genéticos
Seres Humanos
Hipertrigliceridemia/etiologia
Camundongos
Camundongos Knockout
Músculo Estriado/enzimologia
Retroviridae/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
EC 3.1.1.34 (Lipoprotein Lipase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180106
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190963


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[PMID]:29180215
[Au] Autor:Becker RA; Cluff K; Duraisamy N; Casale GP; Pipinos II
[Ad] Endereço:Biomedical Engineering Department, Wichita State University, Wichita, Kansas.
[Ti] Título:Analysis of ischemic muscle in patients with peripheral artery disease using X-ray spectroscopy.
[So] Source:J Surg Res;220:79-87, 2017 Dec.
[Is] ISSN:1095-8673
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Peripheral artery disease (PAD) is a vascular disease caused by atherosclerosis, resulting in decreased blood flow to the lower extremities. The ankle-brachial index (ABI) is a standard PAD diagnostic test but only identifies reduced blood flow based on blood pressure differences. The early signs of PAD manifest themselves not only at a clinical level but also at an elemental and biochemical level. However, the biochemical and elemental alterations to PAD muscle are not well understood. The objective of this study was to compare fundamental changes in intracellular elemental compositions between control, claudicating, and critical limb ischemia muscle tissue. MATERIALS AND METHODS: Gastrocnemius biopsies from three subjects including one control (ABI ≥ 0.9), one claudicating (0.4 ≤ ABI < 0.9), and one critical limb ischemia patient (ABI < 0.4) were evaluated using a scanning electron microscope and energy dispersive X-ray spectroscopy to quantify differences in elemental compositions. Spectra were collected for five myofibers per specimen. An analysis of variance was performed to identify significant differences in muscle elemental compositions. RESULTS: This study revealed that intracellular magnesium and calcium were lower in PAD compared with control myofibers, whereas sulfur was higher. Magnesium and calcium are antagonistic, meaning, if magnesium concentrations go down calcium concentrations should go up. However, our findings do not support this antagonism in PAD. Our analysis found decreases in sodium and potassium, in PAD myofibers. CONCLUSIONS: These findings may provide insight into the pathologic mechanisms that may operate in ischemic muscle and aid in the development of specialized preventive and rehabilitative treatment plans for PAD patients.
[Mh] Termos MeSH primário: Claudicação Intermitente/diagnóstico
Isquemia/diagnóstico
Músculo Estriado/irrigação sanguínea
Doença Arterial Periférica/diagnóstico
[Mh] Termos MeSH secundário: Idoso
Índice Tornozelo-Braço
Biópsia
Progressão da Doença
Eletrólitos/análise
Seres Humanos
Extremidade Inferior
Masculino
Microscopia Eletrônica de Varredura
Meia-Idade
Músculo Estriado/metabolismo
Músculo Estriado/patologia
Músculo Estriado/ultraestrutura
Doença Arterial Periférica/complicações
Doença Arterial Periférica/patologia
Fatores de Risco
Espectrometria por Raios X
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Electrolytes)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171219
[Lr] Data última revisão:
171219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE


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[PMID]:28800593
[Au] Autor:Mussawy H; Viezens L; Hauenherm G; Schroeder M; Schaefer C
[Ad] Endereço:Department of Orthopaedic Surgery, University Medical Centre Hamburg-Eppendorf, Hamburg, Germany.
[Ti] Título:In vivo functional and morphological characterization of bone and striated muscle microcirculation in NSG mice.
[So] Source:PLoS One;12(8):e0183186, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Organ-specific microcirculation plays a central role in tumor growth, tumor cell homing, tissue engineering, and wound healing. Mouse models are widely used to study these processes; however, these mouse strains often possess unique microhemodynamic parameters, making it difficult to directly compare experiments. The full functional characterization of bone and striated muscle microcirculatory parameters in non-obese diabetic-severe combined immunodeficiency/y-chain; NOD-Prkds IL2rg (NSG) mice has not yet been reported. Here, we established either a dorsal skinfold chamber or femur window in NSG mice (n = 23), allowing direct analysis of microcirculatory parameters in vivo by intravital fluorescence microscopy at 7, 14, 21, and 28 days after chamber preparation. Organ-specific differences were observed. Bone had a significantly lower vessel density but a higher vessel diameter than striated muscle. Bone also showed higher effective vascular permeability than striated muscle. The centerline velocity values were similar in the femur window and dorsal skinfold chamber, with a higher volumetric blood flow in bone. Interestingly, bone and striated muscle showed similar tissue perfusion rates. Knowledge of physiological microhemodynamic values of bone and striated muscle in NSG mice makes it possible to analyze pathophysiological processes at these anatomic sites, such as tumor growth, tumor metastasis, and tumor microcirculation, as well as the response to therapeutic agents.
[Mh] Termos MeSH primário: Fêmur/irrigação sanguínea
Microcirculação/fisiologia
Músculo Estriado/irrigação sanguínea
Pele/irrigação sanguínea
[Mh] Termos MeSH secundário: Animais
Velocidade do Fluxo Sanguíneo/fisiologia
Permeabilidade Capilar/fisiologia
Fêmur/anatomia & histologia
Fluoresceína-5-Isotiocianato/análogos & derivados
Fluoresceína-5-Isotiocianato/farmacocinética
Corantes Fluorescentes/farmacocinética
Masculino
Camundongos
Camundongos Endogâmicos NOD
Camundongos Transgênicos
Microscopia de Fluorescência/métodos
Músculo Estriado/anatomia & histologia
Especificidade de Órgãos
Perfusão
Soroalbumina Bovina/farmacocinética
Pele/anatomia & histologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fluorescent Dyes); 0 (fluorescein isothiocyanate bovine serum albumin); 27432CM55Q (Serum Albumin, Bovine); I223NX31W9 (Fluorescein-5-isothiocyanate)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170812
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183186


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[PMID]:28739398
[Au] Autor:Madan A; Thimmaiya D; Franco-Cea A; Aiyaz M; Kumar P; Sparrow JC; Nongthomba U
[Ad] Endereço:Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore 560 012, India. Electronic address: aditimdn@gmail.com.
[Ti] Título:Transcriptome analysis of IFM-specific actin and myosin nulls in Drosophila melanogaster unravels lesion-specific expression blueprints across muscle mutations.
[So] Source:Gene;631:16-28, 2017 Oct 05.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Muscle contraction is a highly fine-tuned process that requires the precise and timely construction of large protein sub-assemblies to form sarcomeres. Mutations in many genes encoding constituent proteins of this macromolecular machine result in defective functioning of the muscle tissue. However, the pathways underlying muscle degeneration, and manifestation of myopathy phenotypes are not well understood. In this study, we explored transcriptional alterations that ensue from the absence of the two major muscle proteins - myosin and actin - using the Drosophila indirect flight muscles. Our aim was to understand how the muscle tissue responds as a whole to the absence of either of the major scaffold proteins, whether the responses are generic to the tissue; or unique to the thick versus thin filament systems. Our results indicated that muscles respond by altering gene transcriptional levels in multiple systems active in muscle remodelling, protein degradation and heat shock responses. However, there were some responses that were filament-specific signatures of muscle degeneration, like immune responses, metabolic alterations and alterations in expression of muscle structural genes and mitochondrial ribosomal genes. These general and filament-specific changes in gene expression may be of relevance to human myopathies.
[Mh] Termos MeSH primário: Actinas/genética
Contração Muscular/genética
Miosinas/genética
[Mh] Termos MeSH secundário: Actinas/metabolismo
Animais
Drosophila
Proteínas de Drosophila/genética
Perfilação da Expressão Gênica
Masculino
Músculo Estriado/fisiologia
Mutação
Miosinas/metabolismo
Análise de Sequência com Séries de Oligonucleotídeos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Actins); 0 (Drosophila Proteins); EC 3.6.4.1 (Myosins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170913
[Lr] Data última revisão:
170913
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE


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[PMID]:28634272
[Au] Autor:Schultz J; Lee SJ; Cole T; Hoang HD; Vibbert J; Cottee PA; Miller MA; Han SM
[Ad] Endereço:Department of Cell, Developmental and Integrative Biology, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
[Ti] Título:The secreted MSP domain of VAPB homolog VPR-1 patterns the adult striated muscle mitochondrial reticulum via SMN-1.
[So] Source:Development;144(12):2175-2186, 2017 06 15.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The major sperm protein domain (MSPd) has an extracellular signaling function implicated in amyotrophic lateral sclerosis. Secreted MSPds derived from the VAPB homolog VPR-1 promote mitochondrial localization to actin-rich I-bands in body wall muscle. Here we show that the nervous system and germ line are key MSPd secretion tissues. MSPd signals are transduced through the CLR-1 Lar-like tyrosine phosphatase receptor. We show that CLR-1 is expressed throughout the muscle plasma membrane, where it is accessible to MSPd within the pseudocoelomic fluid. MSPd signaling is sufficient to remodel the muscle mitochondrial reticulum during adulthood. An RNAi suppressor screen identified survival of motor neuron 1 (SMN-1) as a downstream effector. SMN-1 acts in muscle, where it colocalizes at myofilaments with ARX-2, a component of the Arp2/3 actin-nucleation complex. Genetic studies suggest that SMN-1 promotes Arp2/3 activity important for localizing mitochondria to I-bands. Our results support the model that VAPB homologs are circulating hormones that pattern the striated muscle mitochondrial reticulum. This function is crucial in adults and requires SMN-1 in muscle, likely independent of its role in pre-mRNA splicing.
[Mh] Termos MeSH primário: Proteínas de Caenorhabditis elegans/metabolismo
Caenorhabditis elegans/crescimento & desenvolvimento
Caenorhabditis elegans/metabolismo
Proteínas de Membrana/metabolismo
Músculo Estriado/crescimento & desenvolvimento
Músculo Estriado/metabolismo
Proteínas do Complexo SMN/metabolismo
[Mh] Termos MeSH secundário: Proteína 2 Relacionada a Actina/metabolismo
Complexo 2-3 de Proteínas Relacionadas à Actina/metabolismo
Esclerose Amiotrófica Lateral/metabolismo
Animais
Animais Geneticamente Modificados
Caenorhabditis elegans/genética
Proteínas de Caenorhabditis elegans/química
Proteínas de Caenorhabditis elegans/genética
Genes de Helmintos
Células Germinativas/metabolismo
Seres Humanos
Larva/crescimento & desenvolvimento
Larva/metabolismo
Masculino
Proteínas de Membrana/química
Proteínas de Membrana/genética
Mitocôndrias Musculares/metabolismo
Neurônios Motores/metabolismo
Mutação
Domínios Proteicos
Interferência de RNA
Proteínas Tirosina Fosfatases Semelhantes a Receptores/genética
Proteínas Tirosina Fosfatases Semelhantes a Receptores/metabolismo
Proteínas do Complexo SMN/antagonistas & inibidores
Proteínas do Complexo SMN/genética
Sarcolema/metabolismo
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Actin-Related Protein 2); 0 (Actin-Related Protein 2-3 Complex); 0 (Caenorhabditis elegans Proteins); 0 (Membrane Proteins); 0 (SMN Complex Proteins); 0 (VPR-1 protein, C elegans); 0 (arx-2 protein, C elegans); EC 3.1.3.48 (CLR-1 protein, C elegans); EC 3.1.3.48 (Receptor-Like Protein Tyrosine Phosphatases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170622
[St] Status:MEDLINE
[do] DOI:10.1242/dev.152025


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[PMID]:28559285
[Au] Autor:Lerat H; Imache MR; Polyte J; Gaudin A; Mercey M; Donati F; Baudesson C; Higgs MR; Picard A; Magnan C; Foufelle F; Pawlotsky JM
[Ad] Endereço:From the INSERM, U955, Team "Pathophysiology and Therapy of Chronic Viral Hepatitis and Related Cancers", 94010 Créteil, France, herve.lerat@inserm.fr.
[Ti] Título:Hepatitis C virus induces a prediabetic state by directly impairing hepatic glucose metabolism in mice.
[So] Source:J Biol Chem;292(31):12860-12873, 2017 Aug 04.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Virus-related type 2 diabetes is commonly observed in individuals infected with the hepatitis C virus (HCV); however, the underlying molecular mechanisms remain unknown. Our aim was to unravel these mechanisms using FL-N/35 transgenic mice expressing the full HCV ORF. We observed that these mice displayed glucose intolerance and insulin resistance. We also found that Glut-2 membrane expression was reduced in FL-N/35 mice and that hepatocyte glucose uptake was perturbed, partly accounting for the HCV-induced glucose intolerance in these mice. Early steps of the hepatic insulin signaling pathway, from IRS2 to PDK1 phosphorylation, were constitutively impaired in FL-N/35 primary hepatocytes via deregulation of TNFα/SOCS3. Higher hepatic glucose production was observed in the HCV mice, despite higher fasting insulinemia, concomitant with decreased expression of hepatic gluconeogenic genes. Akt kinase activity was higher in HCV mice than in WT mice, but Akt-dependent phosphorylation of the forkhead transcription factor FoxO1 at serine 256, which triggers its nuclear exclusion, was lower in HCV mouse livers. These findings indicate an uncoupling of the canonical Akt/FoxO1 pathway in HCV protein-expressing hepatocytes. Thus, the expression of HCV proteins in the liver is sufficient to induce insulin resistance by impairing insulin signaling and glucose uptake. In conclusion, we observed a complete set of events leading to a prediabetic state in HCV-transgenic mice, providing a valuable mechanistic explanation for HCV-induced diabetes in humans.
[Mh] Termos MeSH primário: Hepacivirus/patogenicidade
Hepatite C/fisiopatologia
Hepatócitos/virologia
Resistência à Insulina
Estado Pré-Diabético/etiologia
[Mh] Termos MeSH secundário: Absorção Fisiológica
Animais
Linhagem Celular Tumoral
Células Cultivadas
Regulação da Expressão Gênica
Gluconeogênese
Glucose/metabolismo
Transportador de Glucose Tipo 2/genética
Transportador de Glucose Tipo 2/metabolismo
Hepacivirus/genética
Hepacivirus/metabolismo
Hepatite C/metabolismo
Hepatite C/patologia
Hepatite C/virologia
Hepatócitos/metabolismo
Hepatócitos/patologia
Masculino
Camundongos Transgênicos
Músculo Estriado/metabolismo
Músculo Estriado/virologia
Fases de Leitura Aberta
Fosforilação
Estado Pré-Diabético/virologia
Processamento de Proteína Pós-Traducional
RNA/metabolismo
Organismos Livres de Patógenos Específicos
Proteínas Virais/genética
Proteínas Virais/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glucose Transporter Type 2); 0 (RNA, recombinant); 0 (Slc2a2 protein, mouse); 0 (Viral Proteins); 63231-63-0 (RNA); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170825
[Lr] Data última revisão:
170825
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170601
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.785030


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[PMID]:28539306
[Au] Autor:Rassier DE
[Ad] Endereço:Department of Kinesiology and Physical Education, McGill University, Montreal, Quebec, Canada dilson.rassier@mcgill.ca.
[Ti] Título:Sarcomere mechanics in striated muscles: from molecules to sarcomeres to cells.
[So] Source:Am J Physiol Cell Physiol;313(2):C134-C145, 2017 Aug 01.
[Is] ISSN:1522-1563
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Muscle contraction is commonly associated with the cross-bridge and sliding filament theories, which have received strong support from experiments conducted over the years in different laboratories. However, there are studies that cannot be readily explained by the theories, showing ) a plateau of the force-length relation extended beyond optimal filament overlap, and forces produced at long sarcomere lengths that are higher than those predicted by the sliding filament theory; ) passive forces at long sarcomere lengths that can be modulated by activation and Ca , which changes the force-length relation; and ) an unexplained high force produced during and after stretch of activated muscle fibers. Some of these studies even propose "new theories of contraction." While some of these observations deserve evaluation, many of these studies present data that lack a rigorous control and experiments that cannot be repeated in other laboratories. This article reviews these issues, looking into studies that have used intact and permeabilized fibers, myofibrils, isolated sarcomeres, and half-sarcomeres. A common mechanism associated with sarcomere and half-sarcomere length nonuniformities and a Ca -induced increase in the stiffness of titin is proposed to explain observations that derive from these studies.
[Mh] Termos MeSH primário: Conectina/metabolismo
Contração Muscular/fisiologia
Músculo Estriado/fisiologia
Sarcômeros/fisiologia
[Mh] Termos MeSH secundário: Animais
Fenômenos Biomecânicos/fisiologia
Cálcio/metabolismo
Contração Isométrica/fisiologia
Fibras Musculares Esqueléticas/metabolismo
Fibras Musculares Esqueléticas/fisiologia
Músculo Estriado/metabolismo
Miofibrilas/metabolismo
Miofibrilas/fisiologia
Sarcômeros/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Connectin); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170814
[Lr] Data última revisão:
170814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1152/ajpcell.00050.2017


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[PMID]:28526693
[Au] Autor:Batista-Lima FJ; Gadelha KKL; Oliveira DM; Vasconcelos TB; Brito TS; Magalhães PJC
[Ad] Endereço:Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil.
[Ti] Título:A simple laboratory exercise with rat isolated esophagus and stomach fundus to reveal functional differences between striated and smooth muscle cells.
[So] Source:Adv Physiol Educ;41(2):291-297, 2017 Jun 01.
[Is] ISSN:1522-1229
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study describes an undergraduate student laboratory activity using isolated preparations from rat gastrointestinal tissues that possess contractile profiles typically exhibited by striated and smooth muscle cells. While students are introduced to an ex vivo methodology, they can compare differences in trace experiments, twitch aspects, phasic and tonic properties, force-frequency relationships, and pharmacological responsiveness of esophageal (striated) and fundic (smooth muscle) segments. Muscle strips were subjected to electrical field stimulation (EFS) applied by platinum electrodes immersed in the physiological solution. The contractile profile of EFS responses varied between these two types of gut preparations. Atropine and tubocurarine revealed differential inhibitory influences in esophagus or fundus tissues; caffeine and procaine produced similar effects, i.e., potentiation and blockade of the EFS-induced contractile response in these tissues, respectively. Experimental results obtained during the activity helped the improvement of student learning about basic concepts previously discussed in theoretical lectures. To measure student learning with this laboratory exercise, a questionnaire was applied before and after the activity, and the number of expected correct answers, concerning the mechanisms of contraction in striated and smooth muscle, could be clearly evidenced.
[Mh] Termos MeSH primário: Músculo Liso/fisiologia
Músculo Estriado/fisiologia
Fisiologia/educação
[Mh] Termos MeSH secundário: Animais
Estimulação Elétrica
Esôfago/citologia
Técnicas In Vitro
Contração Muscular
Músculo Liso/citologia
Músculo Estriado/citologia
Ratos
Estômago/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170521
[St] Status:MEDLINE
[do] DOI:10.1152/advan.00150.2016


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[PMID]:28421435
[Au] Autor:Popova SS; Vikhlyantsev IM; Zakharova NM; Podlubnaya ZA; Fesenko EE
[Ad] Endereço:Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290, Russia.
[Ti] Título:Seasonal changes in proteolytic activity of calpains in striated muscles of long-tailed ground squirrel Spermophilus undulatus.
[So] Source:Dokl Biochem Biophys;472(1):56-59, 2017 Jan.
[Is] ISSN:1608-3091
[Cp] País de publicação:Russia (Federation)
[La] Idioma:eng
[Ab] Resumo:Seasonal changes in proteolytic activity and content of calpains in striated muscles of the longtailed ground squirrel Spermophilus undulatus were studied by casein zymography and Western blotting analysis. The results testify to hyperactivation of calpain proteases in the skeletal muscles of awakened animals during the "winter" activity. The observed changes are discussed in the context of adaptation of skeletal muscles of long-tailed ground squirrels to hibernation.
[Mh] Termos MeSH primário: Calpaína/metabolismo
Hibernação
Músculo Estriado/enzimologia
Sciuridae/metabolismo
[Mh] Termos MeSH secundário: Animais
Proteólise
Sciuridae/fisiologia
Estações do Ano
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.4.22.- (Calpain)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170420
[St] Status:MEDLINE
[do] DOI:10.1134/S1607672917010148


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[PMID]:28280999
[Au] Autor:Servetto N; Cremonezzi D; Simes JC; Di Pietro A; Campana VR
[Ad] Endereço:Cátedra de Física Biomédica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Santa Rosa 1085, 5000, Córdoba, Argentina.
[Ti] Título:Histomorphologic and ultrastructural recovery of myopathy in rats treated with low-level laser therapy.
[So] Source:Lasers Med Sci;32(4):841-849, 2017 May.
[Is] ISSN:1435-604X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The purpose of the present work was to study the effect of low-level laser therapy (LLLT): helium-neon (He-Ne) and gallium arsenide (Ga-As) laser on the histomorphology of muscle and mitochondria in experimental myopathy in rats. Thirty Suquía strain female rats were distributed in groups: (A) control (intact), (B) injured, (C) injured and treated with He-Ne laser, (D) injured and treated with Ga-As laser, (E) irradiated with He-Ne laser on the non-injured muscle, and (F) irradiated with Ga-As laser on the non-injured muscle. Myopathy was induced by injecting 0.05 mg/rat/day of adrenaline in the left gastrocnemius muscle at the same point on five consecutive days, in groups B, C, and D. LLLT was applied with 9.5 J cm daily for seven consecutive days in groups C, D, E, and F. The muscles were examined with optic and electronic microscopy. The inflammation was classified as absent, mild, and intense and the degree of mitochondrial alteration was graded I, II, III, and IV. Categorical data were statistically analyzed by Chi-square and the Fisher-Irwin Bilateral test, setting significant difference at p < 0.05. The damage found in muscle and mitochondria histomorphology in animals with induced myopathy (B) was intense or severe inflammation with grade III or IV of mitochondrial alteration. They underwent significant regression (p < 0.001) compared with the groups treated with He-Ne (C) and Ga-As (D) laser, in which mild or moderate inflammation was seen and mitochondrial alteration grades I and II, recovering normal myofibrillar architecture. No differences were found between the effects caused by the two lasers, or between groups A, E, and F. Group A was found to be different from B, C, and D (p < 0.001). LLLT in experimental myopathy caused significant muscular and mitochondrial morphologic recovery.
[Mh] Termos MeSH primário: Terapia com Luz de Baixa Intensidade
Músculo Esquelético/patologia
Músculo Esquelético/ultraestrutura
Doenças Musculares/patologia
Doenças Musculares/radioterapia
[Mh] Termos MeSH secundário: Animais
Feminino
Lasers de Gás
Lasers Semicondutores
Mitocôndrias/metabolismo
Mitocôndrias/ultraestrutura
Músculo Estriado/patologia
Músculo Estriado/ultraestrutura
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170311
[St] Status:MEDLINE
[do] DOI:10.1007/s10103-017-2182-1



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