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[PMID]:28452328
[Au] Autor:Golafshan N; Gharibi H; Kharaziha M; Fathi M
[Ad] Endereço:Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.
[Ti] Título:A facile one-step strategy for development of a double network fibrous scaffold for nerve tissue engineering.
[So] Source:Biofabrication;9(2):025008, 2017 04 28.
[Is] ISSN:1758-5090
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to develop a novel double network scaffold composed of polycaprolactone fumarate (PCLF) and eggshell membrane (ESM) (ESM:PCLF) by using the vacuum infiltration method. Compared to ESM, the mechanical properties of double network scaffold were significantly improved, depending on the solvents applied for double network scaffold formation; acetic acid and dichloromethane. Noticeably, the toughness and strength of double network scaffold prepared using acetic acid were significantly improved compared to ESM (26.6 and 25 times, respectively) attributed to the existence of hydrophilic functional groups in acetic acid which made ESM flexible to absorb further PCLF solution. To assess the effect of double network formation on the biological behavior of ESM, the attachment, proliferation and spreading of PC12 cells cultured on the ESM:PCLF scaffolds were evaluated. Results revealed that the number of cells attached on double network ESM:PCLF scaffold were nearly similar to ESM and significantly higher than that of on the tissue culture plate (2.6 times) and PCLF film (1.7 times). It is envisioned that the offered ESM:PCLF double network scaffold might have great potential to develop the constructs for nerve regeneration.
[Mh] Termos MeSH primário: Tecido Nervoso/fisiologia
Engenharia Tecidual
Tecidos Suporte/química
[Mh] Termos MeSH secundário: Animais
Materiais Biocompatíveis/síntese química
Materiais Biocompatíveis/química
Materiais Biocompatíveis/farmacologia
Membrana Celular/química
Movimento Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Casca de Ovo
Interações Hidrofóbicas e Hidrofílicas
Microscopia Eletrônica de Varredura
Regeneração Nervosa/efeitos dos fármacos
Células PC12
Poliésteres/química
Ratos
Espectroscopia de Infravermelho com Transformada de Fourier
Raios Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biocompatible Materials); 0 (Polyesters); 0 (poly(caprolactone fumarate))
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1088/1758-5090/aa68ed


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[PMID]:27775170
[Au] Autor:Kim BC; Jun SM; Kim SY; Kwon YD; Choe SC; Kim EC; Lee JH; Kim J; Suh JF; Hwang YS
[Ad] Endereço:Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Seoul, Korea.
[Ti] Título:Engineering three dimensional micro nerve tissue using postnatal stem cells from human dental apical papilla.
[So] Source:Biotechnol Bioeng;114(4):903-914, 2017 04.
[Is] ISSN:1097-0290
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The in vitro generation of cell-based three dimensional (3D) nerve tissue is an attractive subject to improve graft survival and integration into host tissue for neural tissue regeneration or to model biological events in stem cell differentiation. Although 3D organotypic culture strategies are well established for 3D nerve tissue formation of pluripotent stem cells to study underlying biology in nerve development, cell-based nerve tissues have not been developed using human postnatal stem cells with therapeutic potential. Here, we established a culture strategy for the generation of in vitro cell-based 3D nerve tissue from postnatal stem cells from apical papilla (SCAPs) of teeth, which originate from neural crest-derived ectomesenchyme cells. A stem cell population capable of differentiating into neural cell lineages was generated during the ex vivo expansion of SCAPs in the presence of EGF and bFGF, and SCAPs differentiated into neural cells, showing neural cell lineage-related molecular and gene expression profiles, morphological changes and electrophysical property under neural-inductive culture conditions. Moreover, we showed the first evidence that 3D cell-based nerve-like tissue with axons and myelin structures could be generated from SCAPs via 3D organotypic culture using an integrated bioprocess composed of polyethylene glycol (PEG) microwell-mediated cell spheroid formation and subsequent dynamic culture in a high aspect ratio vessel (HARV) bioreactor. In conclusion, the culture strategy in our study provides a novel approach to develop in vitro engineered nerve tissue using SCAPs and a foundation to study biological events in the neural differentiation of postnatal stem cells. Biotechnol. Bioeng. 2017;114: 903-914. © 2016 Wiley Periodicals, Inc.
[Mh] Termos MeSH primário: Reatores Biológicos
Papila Dentária/citologia
Tecido Nervoso/citologia
Células-Tronco/citologia
Células-Tronco/fisiologia
Engenharia Tecidual/métodos
[Mh] Termos MeSH secundário: Adolescente
Diferenciação Celular
Criança
Seres Humanos
Dente Molar/citologia
Esferoides Celulares/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171216
[Lr] Data última revisão:
171216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE
[do] DOI:10.1002/bit.26205


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[PMID]:28880938
[Au] Autor:Davenport KA; Hoover CE; Bian J; Telling GC; Mathiason CK; Hoover EA
[Ad] Endereço:Prion Research Center, Microbiology, Immunology and Pathology Department, Colorado State University, Fort Collins, Colorado, United States of America.
[Ti] Título:PrPC expression and prion seeding activity in the alimentary tract and lymphoid tissue of deer.
[So] Source:PLoS One;12(9):e0183927, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The agent responsible for prion diseases is a misfolded form of a normal protein (PrPC). The prion hypothesis stipulates that PrPC must be present for the disease to manifest. Cervid populations across the world are infected with chronic wasting disease, a horizontally-transmissible prion disease that is likely spread via oral exposure to infectious prions (PrPCWD). Though PrPCWD has been identified in many tissues, there has been little effort to characterize the overall PrPC expression in cervids and its relationship to PrPCWD accumulation. We used immunohistochemistry (IHC), western blot and enzyme-linked immunosorbent assay to describe PrPC expression in naïve white-tailed deer. We used real-time, quaking-induced conversion (RT-QuIC) to detect prion seeding activity in CWD-infected deer. We assessed tissues comprising the alimentary tract, alimentary-associated lymphoid tissue and systemic lymphoid tissue from 5 naïve deer. PrPC was expressed in all tissues, though expression was often very low compared to the level in the CNS. IHC identified specific cell types wherein PrPC expression is very high. To compare the distribution of PrPC to PrPCWD, we examined 5 deer with advanced CWD infection. Using RT-QuIC, we detected prion seeding activity in all 21 tissues. In 3 subclinical deer sacrificed 4 months post-inoculation, we detected PrPCWD consistently in alimentary-associated lymphoid tissue, irregularly in alimentary tract tissues, and not at all in the brain. Contrary to our hypothesis that PrPC levels dictate prion accumulation, PrPC expression was higher in the lower gastrointestinal tissues than in the alimentary-associated lymphoid system and was higher in salivary glands than in the oropharyngeal lymphoid tissue. These data suggest that PrPC expression is not the sole driver of prion accumulation and that alimentary tract tissues accumulate prions before centrifugal spread from the brain occurs.
[Mh] Termos MeSH primário: Cervos/metabolismo
Trato Gastrointestinal/metabolismo
Tecido Linfoide/metabolismo
Proteínas Priônicas/metabolismo
[Mh] Termos MeSH secundário: Animais
Western Blotting
Ensaio de Imunoadsorção Enzimática
Epitélio/metabolismo
Imuno-Histoquímica
Tecido Nervoso/metabolismo
Doença de Emaciação Crônica/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Prion Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170908
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183927


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[PMID]:28546155
[Au] Autor:Xu Y; Cardell LO
[Ad] Endereço:Division of Ear, Nose, and Throat Diseases, Department of Clinical Sciences, Intervention and Technology, Karolinska Institutet, Stockholm, Sweden; and yuan.xu@ki.se.
[Ti] Título:Long-term nicotine exposure dampens LPS-induced nerve-mediated airway hyperreactivity in murine airways.
[So] Source:Am J Physiol Lung Cell Mol Physiol;313(3):L516-L523, 2017 Sep 01.
[Is] ISSN:1522-1504
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Nicotine is a major component of cigarette smoke. It causes addiction and is used clinically to aid smoke cessation. The aim of the present study is to investigate the effect of nicotine on lipopolysaccharide (LPS)-induced airway hyperreactivity (AHR) and to explore the potential involvement of neuronal mechanisms behind nicotine's effects in murine models in vivo and in vitro. BALB/c mice were exposed to nicotine in vivo via subcutaneous Alzet osmotic minipumps containing nicotine tartate salt solution (24 mg·kg ·day ) for 28 days. LPS (0.1 mg/ml, 20 µl) was administered intranasally for 3 consecutive days during the end of this period. Lung functions were measured with flexiVent. For the in vitro experiments, mice tracheae were organcultured with either nicotine (10 µM) or vehicle (DMSO, 0.1%) for 4 days. Contractile responses of the tracheal segments were measured in myographs following electric field stimulation (EFS; increasing frequencies of 0.2 to 12.8 Hz) before and after incubation with 10 µg/ml LPS for 1 h. Results showed that LPS induced AHR to methacholine in vivo and increased contractile responses to EFS in vitro. Interestingly, long-term nicotine exposure markedly dampened this LPS-induced AHR both in vitro and in vivo. Tetrodotoxin (TTX) inhibited LPS-induced AHR but did not further inhibit nicotine-suppressed AHR in vivo. In conclusion, long-term nicotine exposure dampened LPS-induced AHR. The effect of nicotine was mimicked by TTX, suggesting the involvement of neuronal mechanisms. This information might be used for evaluating the long-term effects of nicotine and further exploring of how tobacco products interact with bacterial airway infections.
[Mh] Termos MeSH primário: Hiper-Reatividade Brônquica/patologia
Pulmão/patologia
Tecido Nervoso/efeitos dos fármacos
Nicotina/farmacologia
[Mh] Termos MeSH secundário: Animais
Líquido da Lavagem Broncoalveolar
Colágeno/metabolismo
Estimulação Elétrica
Feminino
Inflamação/patologia
Lipopolissacarídeos
Pulmão/efeitos dos fármacos
Cloreto de Metacolina/farmacologia
Camundongos
Camundongos Endogâmicos BALB C
Técnicas de Cultura de Órgãos
Mecânica Respiratória/efeitos dos fármacos
Tetrodotoxina/toxicidade
Fatores de Tempo
Receptor 4 Toll-Like/metabolismo
Traqueia/efeitos dos fármacos
Traqueia/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lipopolysaccharides); 0 (Toll-Like Receptor 4); 0W5ETF9M2K (Methacholine Chloride); 4368-28-9 (Tetrodotoxin); 6M3C89ZY6R (Nicotine); 9007-34-5 (Collagen)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170527
[St] Status:MEDLINE
[do] DOI:10.1152/ajplung.00222.2016


  5 / 3210 MEDLINE  
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[PMID]:28534336
[Au] Autor:Shi H; Tian Y; Dai F; Xiao L; Ke Z; Tong W
[Ti] Título:[Establishment of pelvic nerve denervation modal in mice].
[So] Source:Zhonghua Wei Chang Wai Ke Za Zhi;20(5):560-565, 2017 May 25.
[Is] ISSN:1671-0274
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: To establishment and verify pelvic nerve denervation (PND) model in mice. METHODS: (1) Establishment of models. Seventy-two healthy male SPE class C57 mice with age of 7 weeks and body weight of (25±1) g were chosen. These 72 mice were randomly divided into PND group containing 36 mice and sham operation group containing 36 mice. Referring to the establishment method of PND rats, after anesthesia, a laparotomy was performed on the mouse with an abdominal median incision. Under the dissection microscope, the pelvic nerves behind and after each sides of the prostate gland were bluntly separated with cotton swabs and cut with a dissecting scissor. After the operation, the urination of mice was assisted twice every day. For the mice of sham operation group, the pelvic nerves were only exposed without cutting. (2) Detection of models. Colonic transit test was performed in 18 mice chosen randomly from each group to detect the colonic transit ratio (colored colon by methylene blue/ whole colon) and visceral sensitivity tests was performed in the rest mice to observe and record the changes of electromyogram. RESULTS: Three mice died of colonic transit test in each group. Uroschesis occurred in all the mice of PND group and needed bladder massage to assist the urination. Colonic transit test showed that the colonic transit ratios of sham operation group at postoperative day (POD) 1, 3 and 7 were (0.4950±0.3858)%, (0.6386±0.1293)% and (0.6470±0.1088)% without significant difference (F=0.3647, P=0.058), while in PND group, the colonic transit ratio at POD 7 [(0.6044±0.1768) %] was obviously higher than that both at POD 3[(0.3876±0.1364)%, P=0.022] and POD 1[(0.2542±0.0371)%, P=0.001], indicating a recovery trend of colonic transit function (F=9.143, P=0.004). Compared with the sham operation group, the colonic transit function in PND group decreased significantly at POD 1 and POD 3(both P<0.05), and at POD 7, there was no significant difference between two groups. Visceral sensitivity test showed that the visceral sensitivity of sham operation group at POD 1, 3 and 7 was 24.2808±9.5566, 33.6725±7.9548 and 43.9086±12.1875 with significant difference (F=5.722, P=0.014). The visceral sensitivity of PND group at POD 1, 3 and 7 was 11.7609±2.1049, 21.8415±8.1527 and 26.2310±4.2235 with significant difference as well (F=11.154, P=0.001). The visceral sensitivity at POD 3 and POD 7 was obviously higher than that at POD 1 (P=0.006, P<0.001), and there was no significant difference between POD 3 and POD 7 (P=0.183). Compared with sham operation group, the visceral sensitivity of PND group decreased significantly at POD 1, 3 and 7(all P<0.05). CONCLUSIONS: Denervation of pelvic nerves can obviously decrease the colonic transit function and the visceral sensitivity of mice, but these changes can recover over time, which suggests that the establishment of PND model in mice is successful.
[Mh] Termos MeSH primário: Vias Autônomas/cirurgia
Colo/inervação
Denervação/métodos
Modelos Animais de Doenças
Tecido Nervoso/cirurgia
Pelve/inervação
[Mh] Termos MeSH secundário: Dor Abdominal/fisiopatologia
Animais
Vias Autônomas/crescimento & desenvolvimento
Vias Autônomas/fisiopatologia
Colo/fisiopatologia
Trânsito Gastrointestinal/fisiologia
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Tecido Nervoso/crescimento & desenvolvimento
Tecido Nervoso/fisiopatologia
Dor Pós-Operatória/fisiopatologia
Pelve/fisiopatologia
Pelve/cirurgia
Próstata/inervação
Recuperação de Função Fisiológica/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170904
[Lr] Data última revisão:
170904
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170524
[St] Status:MEDLINE


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[PMID]:28524728
[Au] Autor:Damiani AP; Garcez ML; Letieli de Abreu L; Tavares TH; Rodrigues Boeck C; Moraes de Andrade V
[Ad] Endereço:a Laboratório de Biologia Celular e Molecular, Programa de Pós-Graduação em Ciências da Saúde, Unidade Acadêmica de Ciências da Saúde , Universidade do Extremo Sul Catarinense - UNESC , Criciúma , SC , Brazil.
[Ti] Título:A reduction in DNA damage in neural tissue and peripheral blood of old mice treated with caffeine.
[So] Source:J Toxicol Environ Health A;80(13-15):621-629, 2017.
[Is] ISSN:1528-7394
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Studies on caffeine consumption have shown a negative correlation with development of some diseases with subsequent beneficial manifestations. Our aim was to assess the effects of caffeine on peripheral blood and neural tissue DNA in young adult and aged mice. Male Swiss mice (age 2-3 or 16-18 months, respectively) were treated with a caffeine solution (0.3 g/l) for 4 weeks, while controls received water. After the treatments, blood and hippocampal cells (for a comet assay) and femurs (for a micronucleus [MN] test) were collected. The comet assay of peripheral blood and hippocampal cells demonstrated no significant differences between caffeine-treated and control young adult mice in terms of DNA damage index (DI) and frequency. In contrast, when comparing young adult with aged animals, significant differences were observed in DNA damage in blood and hippocampal cells. The differences between aged animals (with or without caffeine) consisted of a significant decrease in DNA DI in the group that received caffeine. In the MN test, an increase in frequency of micronucleated polychromatic (PCE) erythrocytes was noted in aged animals that received water compared to young adult mice. In addition, comparing treated with control aged murine groups, a decrease in frequency of MN was found in PCE erythrocytes of caffeine-treated mice. Chronic caffeine consumption was neither genotoxic nor mutagenic at the dose tested; however, it appears that caffeine actually protected mice from genotoxicity and mutagenicity, consequences attributed to aging.
[Mh] Termos MeSH primário: Sangue/efeitos dos fármacos
Cafeína/farmacologia
Estimulantes do Sistema Nervoso Central/farmacologia
Dano ao DNA/efeitos dos fármacos
Tecido Nervoso/efeitos dos fármacos
[Mh] Termos MeSH secundário: Fatores Etários
Animais
Sangue/metabolismo
Ensaio Cometa
Hipocampo/efeitos dos fármacos
Hipocampo/metabolismo
Masculino
Camundongos
Testes para Micronúcleos
Tecido Nervoso/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Central Nervous System Stimulants); 3G6A5W338E (Caffeine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170520
[St] Status:MEDLINE
[do] DOI:10.1080/15287394.2017.1286901


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[PMID]:28235582
[Au] Autor:Bryant DM; Sousounis K; Farkas JE; Bryant S; Thao N; Guzikowski AR; Monaghan JR; Levin M; Whited JL
[Ad] Endereço:Harvard Medical School, the Harvard stem Cell Institute, and the Department of Orthopedic Surgery, Brigham & Women's Hospital, Cambridge, MA 02139, USA.
[Ti] Título:Repeated removal of developing limb buds permanently reduces appendage size in the highly-regenerative axolotl.
[So] Source:Dev Biol;424(1):1-9, 2017 04 01.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Matching appendage size to body size is fundamental to animal function. Generating an appropriately-sized appendage is a robust process executed during development which is also critical for regeneration. When challenged, larger animals are programmed to regenerate larger limbs than smaller animals within a single species. Understanding this process has important implications for regenerative medicine. To approach this complex question, models with altered appendage size:body size ratios are required. We hypothesized that repeatedly challenging axolotls to regrow limb buds would affect their developmental program resulting in altered target morphology. We discovered that after 10 months following this experimental procedure, limbs that developed were permanently miniaturized. This altered target morphology was preserved upon amputation and regeneration. Future experiments using this platform should provide critical information about how target limb size is encoded within limb progenitors.
[Mh] Termos MeSH primário: Ambystoma mexicanum/embriologia
Amputação
Botões de Extremidades/embriologia
Botões de Extremidades/patologia
[Mh] Termos MeSH secundário: Animais
Ectromelia/patologia
Botões de Extremidades/anormalidades
Botões de Extremidades/inervação
Tecido Nervoso/patologia
Tamanho do Órgão
Regeneração
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171110
[Lr] Data última revisão:
171110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170226
[St] Status:MEDLINE


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[PMID]:28160432
[Au] Autor:Arruda PH; Arruda BL; Schwartz KJ; Vannucci F; Resende T; Rovira A; Sundberg P; Nietfeld J; Hause BM
[Ad] Endereço:Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
[Ti] Título:Detection of a novel sapelovirus in central nervous tissue of pigs with polioencephalomyelitis in the USA.
[So] Source:Transbound Emerg Dis;64(2):311-315, 2017 Apr.
[Is] ISSN:1865-1682
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:An approximately 3,000 finishing swine operation in the United States experienced an outbreak of an atypical neurologic disease in 11-weeks-old pigs with an overall morbidity of 20% and case fatality rate of 30%. The clinical onset and progression of signs in affected pigs varied but included inappetence, compromised ambulation, ataxia, incoordination, mental dullness, paresis, paralysis and decreased response to environmental stimuli. Tissues from affected pigs were submitted for diagnostic investigation. Histopathologic examination of the cerebrum, cerebellum and spinal cord revealed severe lymphoplasmacytic and necrotizing polioencephalomyelitis with multifocal areas of gliosis and neuron satellitosis, suggestive of a neurotropic viral infection. Bacterial pathogens were not isolated by culture of neurologic tissue from affected pigs. Samples tested by polymerase chain reaction (PCR) were negative for pseudorabies virus and atypical porcine pestivirus. Immunohistochemistry for porcine reproductive and respiratory syndrome virus, porcine circovirus and Listeria was negative. Porcine sapelovirus (PSV) was identified in spinal cord by a nested PCR used to detect porcine enterovirus, porcine teschovirus and PSV. Next-generation sequencing of brainstem and spinal cord samples identified PSV and the absence of other or novel pathogens. In addition, Sapelovirus A mRNA was detected in neurons and nerve roots of the spinal cord by in situ hybridization. The PSV is genetically novel with an overall 94% amino acid identity and 86% nucleotide identity to a recently reported sapelovirus from Korea. This is the first case report in the United States associating sapelovirus with severe polioencephalomyelitis in pigs.
[Mh] Termos MeSH primário: Infecções por Circoviridae/epidemiologia
Encefalomielite Enzoótica Suína/virologia
Infecções por Enterovirus/veterinária
Infecções por Picornaviridae/veterinária
Picornaviridae/isolamento & purificação
Doenças dos Suínos/virologia
[Mh] Termos MeSH secundário: Animais
Surtos de Doenças
Infecções por Enterovirus/virologia
Enterovirus Suínos/isolamento & purificação
Imuno-Histoquímica
Hibridização In Situ
Tecido Nervoso/virologia
Picornaviridae/genética
Reação em Cadeia da Polimerase
Vírus de RNA
Suínos
Doenças dos Suínos/epidemiologia
Estados Unidos/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170620
[Lr] Data última revisão:
170620
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170205
[St] Status:MEDLINE
[do] DOI:10.1111/tbed.12621


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[PMID]:28069509
[Au] Autor:Simitzi C; Ranella A; Stratakis E
[Ad] Endereço:Institute of Electronic Structure and Laser (IESL), Foundation for Research and Technology-Hellas (FORTH), Heraklion 71003, Greece.
[Ti] Título:Controlling the morphology and outgrowth of nerve and neuroglial cells: The effect of surface topography.
[So] Source:Acta Biomater;51:21-52, 2017 Mar 15.
[Is] ISSN:1878-7568
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Unlike other tissue types, like epithelial tissue, which consist of cells with a much more homogeneous structure and function, the nervous tissue spans in a complex multilayer environment whose topographical features display a large spectrum of morphologies and size scales. Traditional cell cultures, which are based on two-dimensional cell-adhesive culture dishes or coverslips, are lacking topographical cues and mainly simulate the biochemical microenvironment of the cells. With the emergence of micro- and nano-fabrication techniques new types of cell culture platforms are developed, where the effect of various topographical cues on cellular morphology, proliferation and differentiation can be studied. Different approaches (regarding the material, fabrication technique, topographical characteristics, etc.) have been implemented. The present review paper aims at reviewing the existing body of literature on the use of artificial micro- and nano-topographical features to control neuronal and neuroglial cells' morphology, outgrowth and neural network topology. The cell responses-from phenomenology to investigation of the underlying mechanisms- on the different topographies, including both deterministic and random ones, are summarized. STATEMENT OF SIGNIFICANCE: There is increasing evidence that physical cues, such as topography, can have a significant impact on the neural cell functions. With the aid of micro-and nanofabrication techniques, new types of cell culture platforms are developed and the effect of surface topography on the cells has been studied. The present review article aims at reviewing the existing body of literature reporting on the use of various topographies to study and control the morphology and functions of cells from nervous tissue, i.e. the neuronal and the neuroglial cells. The cell responses-from phenomenology to investigation of the underlying mechanisms- on the different topographies, including both deterministic and random ones, are summarized.
[Mh] Termos MeSH primário: Forma Celular
Tecido Nervoso/citologia
Neuroglia/citologia
[Mh] Termos MeSH secundário: Animais
Materiais Biocompatíveis/farmacologia
Forma Celular/efeitos dos fármacos
Seres Humanos
Neuritos/efeitos dos fármacos
Neuritos/metabolismo
Neuroglia/efeitos dos fármacos
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Biocompatible Materials)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170111
[St] Status:MEDLINE


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[PMID]:27974554
[Au] Autor:Huang CY; Yao HW; Wang LC; Shen FH; Hsu SM; Chen SH
[Ad] Endereço:Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Republic of China.
[Ti] Título:Thymidine Kinase-Negative Herpes Simplex Virus 1 Can Efficiently Establish Persistent Infection in Neural Tissues of Nude Mice.
[So] Source:J Virol;91(4), 2017 Feb 15.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Herpes simplex virus 1 (HSV-1) establishes latency in neural tissues of immunocompetent mice but persists in both peripheral and neural tissues of lymphocyte-deficient mice. Thymidine kinase (TK) is believed to be essential for HSV-1 to persist in neural tissues of immunocompromised mice, because infectious virus of a mutant with defects in both TK and UL24 is detected only in peripheral tissues, but not in neural tissues, of severe combined immunodeficiency mice (T. Valyi-Nagy, R. M. Gesser, B. Raengsakulrach, S. L. Deshmane, B. P. Randazzo, A. J. Dillner, and N. W. Fraser, Virology 199:484-490, 1994, https://doi.org/10.1006/viro.1994.1150). Here we find infiltration of CD4 and CD8 T cells in peripheral and neural tissues of mice infected with a TK-negative mutant. We therefore investigated the significance of viral TK and host T cells for HSV-1 to persist in neural tissues using three genetically engineered mutants with defects in only TK or in both TK and UL24 and two strains of nude mice. Surprisingly, all three mutants establish persistent infection in up to 100% of brain stems and 93% of trigeminal ganglia of adult nude mice at 28 days postinfection, as measured by the recovery of infectious virus. Thus, in mouse neural tissues, host T cells block persistent HSV-1 infection, and viral TK is dispensable for the virus to establish persistent infection. Furthermore, we found 30- to 200-fold more virus in neural tissues than in the eye and detected glycoprotein C, a true late viral antigen, in brainstem neurons of nude mice persistently infected with the TK-negative mutant, suggesting that adult mouse neurons can support the replication of TK-negative HSV-1. IMPORTANCE: Acyclovir is used to treat herpes simplex virus 1 (HSV-1)-infected immunocompromised patients, but treatment is hindered by the emergence of drug-resistant viruses, mostly those with mutations in viral thymidine kinase (TK), which activates acyclovir. TK mutants are detected in brains of immunocompromised patients with persistent infection. However, answers to the questions as to whether TK-negative (TK ) HSV-1 can establish persistent infection in brains of immunocompromised hosts and whether neurons in vivo are permissive for TK HSV-1 remain elusive. Using three genetically engineered HSV-1 TK mutants and two strains of nude mice deficient in T cells, we found that all three HSV-1 TK mutants can efficiently establish persistent infection in the brain stem and trigeminal ganglion and detected glycoprotein C, a true late viral antigen, in brainstem neurons. Our study provides evidence that TK HSV-1 can persist in neural tissues and replicate in brain neurons of immunocompromised hosts.
[Mh] Termos MeSH primário: Herpes Simples/virologia
Herpesvirus Humano 1/fisiologia
Tecido Nervoso/virologia
Timidina Quinase/genética
Proteínas Virais/genética
[Mh] Termos MeSH secundário: Animais
Tronco Encefálico/metabolismo
Tronco Encefálico/virologia
Linhagem Celular
Modelos Animais de Doenças
Herpes Simples/imunologia
Herpes Simples/patologia
Seres Humanos
Camundongos
Camundongos Nus
Mutação
Subpopulações de Linfócitos T/imunologia
Subpopulações de Linfócitos T/metabolismo
Timidina Quinase/deficiência
Gânglio Trigeminal/metabolismo
Gânglio Trigeminal/virologia
Carga Viral
Latência Viral
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Proteins); EC 2.7.1.21 (Thymidine Kinase)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161216
[St] Status:MEDLINE



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