Base de dados : MEDLINE
Pesquisa : A10.806 [Categoria DeCS]
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[PMID]:29183009
[Au] Autor:Li B; Li C; Zhu M; Zhang Y; Du J; Xu Y; Liu B; Gao F; Liu H; Cai J; Yang Y
[Ad] Endereço:Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, China.
[Ti] Título:Hypoxia-Induced Mesenchymal Stromal Cells Exhibit an Enhanced Therapeutic Effect on Radiation-Induced Lung Injury in Mice due to an Increased Proliferation Potential and Enhanced Antioxidant Ability.
[So] Source:Cell Physiol Biochem;44(4):1295-1310, 2017.
[Is] ISSN:1421-9778
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND/AIMS: Radiation therapy is an important treatment for thoracic cancer; however, side effects accompanied with radiotherapy lead to limited tumor control and a decline in patient quality of life. Among these side effects, radiation-induced lung injury (RILI) is the most serious and common. Hence, an effective remedy for RILI is needed. Mesenchymal stromal cells (MSCs) are multipotent adult stem cells that have been demonstrated to be an effective treatment in some disease caused by tissue damage. However, unlike other injuries, RILI received limited therapeutic effects from implanted MSCs due to local hypoxia and extensive reactive oxygen species (ROS) in irradiated lungs. Since the poor survival of MSCs is primarily due to hypoxia and ROS generation, we hypothesize that persistent and adaptive hypoxia treatment induces enhanced resistance to hypoxic stress in implanted MSC. The aim of this study is to investigate whether persistent and adaptive hypoxia treatment of bmMSCs prior to their transplantation in injured mice enhanced survival and improved curative effects in RILI. METHODS: Primary bmMSCs were obtained from the marrow of six-week-old male C57BL6/J mice and were cultured either under normoxic conditions (21% O2) or hypoxic conditions (2.5% O2). Mice were injected with normoxia/hypoxia MSCs after thoracic irradiation (20 Gy). The therapeutic effects of MSCs on RILI were assessed by pathological examinations that included H&E staining, Masson staining and α-SMA staining; meanwhile, inflammatory factors were measured using an ELISA. The morphology of MSCs in vitro was recorded using a microscope and identified by flow cytometry, cell viability was measured using the CCK-8 assay, the potential for proliferation was detected by the EdU assay, and ROS levels were measured using a ROS fluorogenic probe. In addition, HIF-1α and several survival pathway proteins (Akt, p-Akt, Caspase-3) were also detected by western blotting. RESULTS: Implanted MSCs alleviated both early radiation-induced pneumonia and late pulmonary fibrosis. However, hypoxia MSCs displayed a more pronounced therapeutic effect compared to normoxia MSCs. Compared to normoxia MSCs, the hypoxia MSCs demonstrated greater cell viability, an enhanced proliferation potential, decreased ROS levels and increased resistance to hypoxia and ROS stress. In addition, hypoxia MSCs achieved higher activation levels of HIF-1α and Akt, and HIF-1α played a critical role in the development of resistance. CONCLUSION: Hypoxia enhances the therapeutic effect of mesenchymal stromal cells on radiation-induced lung injury by promoting MSC proliferation and improving their antioxidant ability, mediated by HIF-1α.
[Mh] Termos MeSH primário: Antioxidantes/metabolismo
Hipóxia Celular
Lesão Pulmonar/terapia
Transplante de Células-Tronco Mesenquimais
[Mh] Termos MeSH secundário: Actinas/genética
Actinas/metabolismo
Animais
Apoptose/efeitos da radiação
Células da Medula Óssea/citologia
Caspase 3/metabolismo
Proliferação Celular
Sobrevivência Celular
Células Cultivadas
Raios gama
Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo
Lesão Pulmonar/patologia
Masculino
Células Mesenquimais Estromais/citologia
Células Mesenquimais Estromais/metabolismo
Camundongos
Camundongos Endogâmicos C57BL
Tecido Parenquimatoso/citologia
Tecido Parenquimatoso/metabolismo
Tecido Parenquimatoso/efeitos da radiação
Proteínas Proto-Oncogênicas c-akt/metabolismo
Espécies Reativas de Oxigênio/metabolismo
Fator de Crescimento Transformador beta/análise
Fator de Crescimento Transformador beta/metabolismo
Fator de Necrose Tumoral alfa/análise
Fator de Necrose Tumoral alfa/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Actins); 0 (Antioxidants); 0 (Hypoxia-Inducible Factor 1, alpha Subunit); 0 (Reactive Oxygen Species); 0 (Transforming Growth Factor beta); 0 (Tumor Necrosis Factor-alpha); 0 (alpha-smooth muscle actin, mouse); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt); EC 3.4.22.- (Caspase 3)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180118
[Lr] Data última revisão:
180118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE
[do] DOI:10.1159/000485490


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[PMID]:28462988
[Au] Autor:Nikan M; Osborn MF; Coles AH; Biscans A; Godinho BMDC; Haraszti RA; Sapp E; Echeverria D; DiFiglia M; Aronin N; Khvorova A
[Ti] Título:Synthesis and Evaluation of Parenchymal Retention and Efficacy of a Metabolically Stable O-Phosphocholine-N-docosahexaenoyl-l-serine siRNA Conjugate in Mouse Brain.
[So] Source:Bioconjug Chem;28(6):1758-1766, 2017 06 21.
[Is] ISSN:1520-4812
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ligand-conjugated siRNAs have the potential to achieve targeted delivery and efficient silencing in neurons following local administration in the central nervous system (CNS). We recently described the activity and safety profile of a docosahexaenoic acid (DHA)-conjugated, hydrophobic siRNA (DHA-hsiRNA) targeting Huntingtin (Htt) mRNA in mouse brain. Here, we report the synthesis of an amide-modified, phosphocholine-containing DHA-hsiRNA conjugate (PC-DHA-hsiRNA), which closely resembles the endogenously esterified biological structure of DHA. We hypothesized that this modification may enhance neuronal delivery in vivo. We demonstrate that PC-DHA-hsiRNA silences Htt in mouse primary cortical neurons and astrocytes. After intrastriatal delivery, Htt-targeting PC-DHA-hsiRNA induces ∼80% mRNA silencing and 71% protein silencing after 1 week. However, PC-DHA-hsiRNA did not substantially outperform DHA-hsiRNA under the conditions tested. Moreover, at the highest locally administered dose (4 nmol, 50 µg), we observe evidence of PC-DHA-hsiRNA-mediated reactive astrogliosis. Lipophilic ligand conjugation enables siRNA delivery to neural tissues, but rational design of functional, nontoxic siRNA conjugates for CNS delivery remains challenging.
[Mh] Termos MeSH primário: Encéfalo/metabolismo
Sistemas de Liberação de Medicamentos/métodos
Tecido Parenquimatoso/metabolismo
RNA Interferente Pequeno/síntese química
[Mh] Termos MeSH secundário: Animais
Encéfalo/patologia
Ácidos Docosa-Hexaenoicos/química
Estabilidade de Medicamentos
Inativação Gênica
Proteína Huntingtina/genética
Camundongos
Fosforilcolina/química
Interferência de RNA
RNA Mensageiro
RNA Interferente Pequeno/administração & dosagem
RNA Interferente Pequeno/uso terapêutico
Serina/química
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Huntingtin Protein); 0 (RNA, Messenger); 0 (RNA, Small Interfering); 107-73-3 (Phosphorylcholine); 25167-62-8 (Docosahexaenoic Acids); 452VLY9402 (Serine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171219
[Lr] Data última revisão:
171219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1021/acs.bioconjchem.7b00226


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[PMID]:27771090
[Au] Autor:Weller MMDCA; Albino RL; Marcondes MI; Silva W; Daniels KM; Campos MM; Duarte MS; Mescouto ML; Silva FF; Guimarães SEF
[Ad] Endereço:Animal Science Department, Universidade Federal de Viçosa, Minas Gerais, Brazil 36570-000.
[Ti] Título:Effects of nutrient intake level on mammary parenchyma growth and gene expression in crossbred (Holstein × Gyr) prepubertal heifers.
[So] Source:J Dairy Sci;99(12):9962-9973, 2016 Dec.
[Is] ISSN:1525-3198
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study investigated the effects of increased nutrient intake levels on prepubertal mammary parenchyma development in crossbreed (Holstein × Gyr) dairy heifers. Eighteen heifers age 3 to 4 mo were fed 1 of 3 nutrient intake levels (n=6 per treatment) designed to sustain an average daily gain of 0.0kg/d (maintenance, MA), 0.5kg/d (low gain, LG), or 1.0kg/d (high gain, HG). Serum blood samples collected on d 42 and 84 after a 12-h fast were analyzed for triglycerides, leptin, insulin, and insulin-like growth factor 1 (IGF-1). Liver and mammary parenchyma were biopsied on d 42 and harvested on d 84 for gene expression analysis. Parenchyma samples were also used for biochemical and histological analysis. Mammary parenchyma weight was lower in HG than in MA or LG heifers, but mammary extraparenchymal fat was greater in HG heifers than in other groups. Heifers fed the HG diet had a greater fraction of ether extract in their parenchyma than the others and a smaller fraction of crude protein in their parenchyma than MA heifers. Moreover, the HG and LG heifers had greater body fat mass than MA heifers. Nutrient intake level had no effect on the number of intraparenchymal adipocytes. Heifers fed the HG diet had greater serum IGF-1 than the others, and serum insulin was lower in the MA than the HG or LG heifers. Liver GHR, IGF1, and IGFBP3 mRNA expression was higher, but IGFBP2 mRNA was lower in HG heifers than in others. The parenchyma mRNA expression of lipogenic markers, such as CD36, ACCA, FASN, and ADIPOR1, was upregulated by nutrient intake level. Significant nutrient intake × time interactions for lipogenic genes during the experimental period indicated variable gene expression depending on the time point of prepubertal mammary gland development. Overall, our data suggest that enhancing nutrient intake increased body fat accumulation and lipogenesis in the mammary gland to the detriment of parenchyma growth. Moreover, increased lipogenesis in the parenchyma of HG heifers may indicate that fat accumulation occurred because of adipocyte hypertrophy and not differences in adipogenesis. The implications of these results for milk yield needs to be elucidated.
[Mh] Termos MeSH primário: Bovinos/fisiologia
Dieta/veterinária
Regulação da Expressão Gênica
Fígado/metabolismo
Glândulas Mamárias Animais/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Animais
Bovinos/genética
Bovinos/crescimento & desenvolvimento
Ingestão de Energia
Feminino
Glândulas Mamárias Animais/metabolismo
Tecido Parenquimatoso/crescimento & desenvolvimento
Tecido Parenquimatoso/metabolismo
Distribuição Aleatória
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171204
[Lr] Data última revisão:
171204
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


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[PMID]:28952698
[Au] Autor:Popov SV; Guseinov RG; Martov AG; Muratov TM; Tabynbaev NB
[Ad] Endereço:St. Lukes Clinical Hospital, St. Petersburg, Russia.
[Ti] Título:[Molecular and cellular mechanisms of damage to renal parenchyma in renal warm ischemia].
[So] Source:Urologiia;(4):79-84, 2017 Sep.
[Is] ISSN:1728-2985
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Warm ischemia of the renal parenchyma is a forced feature of laparoscopic partial nephrectomy. It is accompanied by oxygen deprivation of the organ and followed by re-oxygenation, which can cause additional damage to the renal tissue. This damage can result in acute functional and structural disorders of individual parts of the nephron, increasing the risk for a renal dysfunction. Timely diagnosis of the dysfunction is vital for the success of the treatment. The article provides an overview of current scientific data on the mechanisms of ischemic and reperfusion injuries at the molecular-cellular level and describes the current methods of their detection. Experimental and clinical study of the molecular-cellular mechanisms of ischemic-reperfusion injury of the renal tissue made it possible, first, to determine the main targets of alteration (cytolemma, mitochondria, lysosomes), and second, to establish its consequences, among which the most important are hypoergosis, DNA damage, simultaneous activation of intracellular systems of the suicidal program and induction of electrical breakdown of membranes of target nephrocytes; thirdly, to reveal the range of possibilities for limiting the consequences of hypoxia and/or re-oxygenation, among which interference in the metabolism of purines, measures ensuring the preservation of colloid osmotic pressure inside and outside the cell and membrane stabilization, antioxidant defense and inhibition of cysteine proteinases, etc. However, despite the advances in understanding the pathogenesis of cell damage, including ischemic-hypoxic injury, the problem of intraoperative ischemia-reperfusion safety remains relevant.
[Mh] Termos MeSH primário: Rim/patologia
Tecido Parenquimatoso/patologia
Traumatismo por Reperfusão/patologia
Isquemia Quente/efeitos adversos
[Mh] Termos MeSH secundário: Animais
Apoptose
Cálcio/metabolismo
Calpaína/metabolismo
Hipóxia Celular
Radicais Livres/metabolismo
Seres Humanos
Espaço Intracelular/metabolismo
Rim/irrigação sanguínea
Rim/metabolismo
Tecido Parenquimatoso/metabolismo
Proteólise
Traumatismo por Reperfusão/etiologia
Traumatismo por Reperfusão/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Free Radicals); EC 3.4.22.- (Calpain); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170928
[St] Status:MEDLINE


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[PMID]:28935346
[Au] Autor:Stamatis G; Fechner S; Rocha M; Weinreich G
[Ad] Endereço:Department of Thoracic Surgery and Endoscopy, Ruhrlandklinik, West German Lung Center, University Hospital Essen, University Duisburg-Essen, Essen, Germany. Electronic address: georgios.stamatis@ruhrlandklinik.uk-essen.de.
[Ti] Título:Resection of the Tracheobronchial Bifurcation With Complete Preservation of Lung Parenchyma.
[So] Source:Ann Thorac Surg;104(5):1741-1747, 2017 Nov.
[Is] ISSN:1552-6259
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The resection of the tracheobronchial bifurcation with complete preservation of lung parenchyma remains a challenge owing to the limited indications for surgery, anesthesiologic management, operative technique, and postoperative course. The aim of this retrospective study was to evaluate factors influencing the perioperative course and long-term survival. METHODS: Between 1989 and 2014, 19 patients underwent a resection of the distal trachea and carina with complete preservation of lung tissue, 16 for malignant tumors (7 adenoid cystic carcinomas, 3 carcinoid tumors, 3 mucoepidermoid tumors, 2 squamous cell carcinomas, and 1 small cell carcinomas), 2 for inflammatory stenosis, and 1 after a complex traumatic rupture. RESULTS: Surgical approach was posterolateral thoracotomy in 17 patients and median sternotomy in 2. In 16 patients, end-to-end anastomosis was performed, and in 3 patients, combined end-to-end and side-to-end anastomosis were performed. The operative mortality was 0%, the perioperative complication rate was 26.3%. Six patients with adenoid cystic carcinoma and all patients with lung carcinoma received adjuvant radiotherapy; only 1 patient with small cell lung cancer had chemotherapy before surgery. Long-term results are excellent in patients with benign disease, typical and atypical carcinoid tumor, mucoepidermoid carcinoma, and in most patients with adenoid cystic carcinoma. Two patients with lung cancer died 28 and 45 months after surgery, and 1 patient with adenoid cystic carcinoma died 75 months after surgery. CONCLUSIONS: Resection of the tracheobronchial bifurcation with complete preservation of lung indicated for selected patients with local tumor growth at the distal trachea and carina provides low perioperative mortality and complications and results in long-term survival rates.
[Mh] Termos MeSH primário: Neoplasias Brônquicas/patologia
Neoplasias Brônquicas/cirurgia
Tratamentos com Preservação do Órgão/métodos
Pneumonectomia/métodos
Neoplasias da Traqueia/patologia
Neoplasias da Traqueia/cirurgia
[Mh] Termos MeSH secundário: Adulto
Biópsia por Agulha
Neoplasias Brônquicas/diagnóstico por imagem
Neoplasias Brônquicas/mortalidade
Estudos de Coortes
Feminino
Seres Humanos
Imagem Tridimensional
Imuno-Histoquímica
Masculino
Meia-Idade
Tecido Parenquimatoso
Prognóstico
Estudos Retrospectivos
Medição de Risco
Taxa de Sobrevida
Tomografia Computadorizada por Raios X/métodos
Neoplasias da Traqueia/diagnóstico por imagem
Neoplasias da Traqueia/mortalidade
Resultado do Tratamento
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170923
[St] Status:MEDLINE


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[PMID]:28877349
[Au] Autor:Boxum AG; La Bastide-Van Gemert S; Dijkstra LJ; Hamer EG; Hielkema T; Reinders-Messelink HA; Hadders-Algra M
[Ad] Endereço:University of Groningen, University Medical Center Groningen, Department of Paediatrics - Developmental Neurology, Groningen, the Netherlands.
[Ti] Título:Development of the quality of reaching in infants with cerebral palsy: a kinematic study.
[So] Source:Dev Med Child Neurol;59(11):1164-1173, 2017 Nov.
[Is] ISSN:1469-8749
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIM: To assess development of reaching and head stability in infants at very high risk (VHR-infants) of cerebral palsy (CP) who did and did not develop CP. METHOD: This explorative longitudinal study assessed the kinematics of reaching and head sway in sitting in 37 VHR-infants (18 CP) one to four times between 4.7 months and 22.6 months corrected age. Developmental trajectories were calculated using linear mixed effect models. Motor function was evaluated with the Infant Motor Profile (IMP) around 13 months corrected age. RESULTS: Throughout infancy, VHR-infants with CP had a worse reaching quality than infants without CP, reflected for example by more movement units (factor 1.52, 95% CI 1.16-1.99) and smaller transport movement units (factor 1.86, 95% CI 1.20-2.90). Total head sway of infants with and without CP was similar, but infants with CP used more head movement units to achieve stability. The rate of developmental change in infants with and without CP was similar. Around 13 months, head control and reaching quality were interrelated; both were associated with IMP-scores. INTERPRETATION: Infants with CP showed a worse kinematic reaching quality and head stability throughout infancy from early age onwards than VHR-infants without CP, implying that kinematically they do not grow into a deficit, but exhibit deficits from early infancy on. WHAT THIS PAPER ADDS: Reaching quality improves throughout infancy in all infants at high risk (VHR-infants). Infants with cerebral palsy (CP) show a worse reaching quality than VHR-infants without CP. Infants with CP achieve head stability differently from infants without CP. Infants with CP exhibit kinematic reaching problems from early age onwards.
[Mh] Termos MeSH primário: Paralisia Cerebral/complicações
Transtornos dos Movimentos/etiologia
Amplitude de Movimento Articular/fisiologia
[Mh] Termos MeSH secundário: Fatores Etários
Fenômenos Biomecânicos
Feminino
Seres Humanos
Lactente
Leucomalácia Periventricular/complicações
Modelos Lineares
Estudos Longitudinais
Masculino
Exame Neurológico
Tecido Parenquimatoso/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE
[do] DOI:10.1111/dmcn.13538


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[PMID]:28787016
[Au] Autor:Boser SR; Mauad T; Araújo-Paulino BB; Mitchell I; Shrestha G; Chiu A; Butt J; Kelly MM; Caldini E; James A; Green FHY
[Ad] Endereço:Airway Inflammation Group, Snyder Institute of Chronic Diseases, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada.
[Ti] Título:Myofibroblasts are increased in the lung parenchyma in asthma.
[So] Source:PLoS One;12(8):e0182378, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Increased airway smooth muscle is observed in large and small airways in asthma. Semi-quantitative estimates suggest that cells containing alpha smooth muscle actin (α-SMA) are also increased in the lung parenchyma. This study quantified and characterized α-SMA positive cells (α-SMA+) in the lung parenchyma of non-asthmatic and asthmatic individuals. METHODS: Post-mortem sections of peripheral lung from cases of fatal asthma (FA), persons with asthma dying of non-respiratory causes (NFA) and non-asthma control subjects (NAC) were stained for α-SMA, quantified using point-counting and normalised to alveolar basement membrane length and interstitial area. RESULTS: α-SMA+ fractional area was increased in alveolar parenchyma in both FA (14.7 ± 2.8% of tissue area) and NFA (13.0 ± 1.2%), compared with NAC (7.4 ± 2.4%), p < 0.05 The difference was greater in upper lobes compared with lower lobes (p < 0.01) in both asthma groups. Similar changes were observed in alveolar ducts and alveolar walls. The electron microscopic features of the α-SMA+ cells were characteristic of myofibroblasts. CONCLUSIONS: We conclude that in asthma there is a marked increase in α-SMA+ myofibroblasts in the lung parenchyma. The physiologic consequences of this increase are unknown.
[Mh] Termos MeSH primário: Asma/patologia
Pulmão/patologia
Miofibroblastos/patologia
Tecido Parenquimatoso/patologia
[Mh] Termos MeSH secundário: Actinas/metabolismo
Adolescente
Adulto
Contagem de Células
Feminino
Seres Humanos
Masculino
Meia-Idade
Miócitos de Músculo Liso/metabolismo
Miócitos de Músculo Liso/patologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Actins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170809
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182378


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Collaço, Luiz Martins
Texto completo SciELO Brasil
[PMID]:28767916
[Au] Autor:Wiederkehr HA; Wiederkehr JC; Collaço LM; Sousa EL; Salvalaggio P; Carvalho CA; Wiederkehr BA; Marques CAM; Rosa FFD; Nanni FN; Fuchs T
[Ad] Endereço:Faculdade Evangélica do Paraná, Curitiba, PR, Brazil.
[Ti] Título:Transection of the hepatic parenchyma associated or not with the contralateral portal vein branch ligature and its effect in liver regeneration.
[So] Source:Einstein (Sao Paulo);15(2):178-185, 2017 Apr-Jun.
[Is] ISSN:2317-6385
[Cp] País de publicação:Brazil
[La] Idioma:eng; por
[Ab] Resumo:Objective: To analyze the influence of portal vein ligation in hepatic regeneration by immunohistochemical criteria. Methods: Ten pigs divided into two groups of five animals underwent hepatectomy in two stages, and the groups were differentiated by ligation or not of the left portal vein tributary, which is responsible for vascularization of the left lateral and medial lobes of the pig liver. Five days after the procedure, the animals underwent liver biopsies for further analysis of histological and immunohistochemical with marker Ki67. Results: The group submitted to hepatectomy with vascular ligation showed an increase of approximately 4% of hepatocytes in regeneration status, as well as a greater presence of Kupffer and inflammatory cells as compared to control. Conclusion: As a result of positive cell replication observed through the Ki67 marker, we can suspect that the ligation of a tributary of the portal vein associated with liver resection promoted a greater stimulus of liver regeneration when compared to liver resection alone. Objetivo: Analisar a influência da ligadura da tributária da veia porta no estímulo regenerativo hepático por meio de critérios imuno-histoquímicos. Métodos: Dez suínos, divididos em dois grupos de cinco animais, foram submetidos à hepatectomia em dois estágios, sendo que os grupos foram diferenciados pela ligadura ou não da tributária da veia porta, responsável pela vascularização dos lobos lateral e medial esquerdos do fígado do suíno. Cinco dias após o procedimento, os animais foram reabordados para retirada de amostras hepáticas para posterior análise de histológica e imunoistoquímica com o marcador Ki67. Resultados: O grupo submetido à hepatectomia com ligadura vascular apresentou incremento de 4% aproximadamente de hepatócitos em processo de regeneração, bem como grande número de células de Kupffer e células inflamatórias, quando comparado ao controle. Conclusão: Em virtude da análise positiva da replicação celular observada por meio do marcador Ki67, pode-se observar que a ligadura de uma tributária da veia porta promoveu um maior estímulo de regeneração hepática, efeito observado com menor intensidade no grupo submetido apenas à ressecção hepática.
[Mh] Termos MeSH primário: Hepatectomia/métodos
Regeneração Hepática
Fígado/cirurgia
Tecido Parenquimatoso/cirurgia
Veia Porta/cirurgia
[Mh] Termos MeSH secundário: Animais
Hepatócitos/metabolismo
Antígeno Ki-67/metabolismo
Leucócitos
Ligadura/métodos
Fígado/patologia
Modelos Animais
Tecido Parenquimatoso/patologia
Distribuição Aleatória
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ki-67 Antigen)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170803
[St] Status:MEDLINE


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[PMID]:28727768
[Au] Autor:Rosenzweig B; Rubinstein ND; Reznik E; Shingarev R; Juluru K; Akin O; Hsieh JJ; Jaimes EA; Russo P; Susztak K; Coleman JA; Hakimi AA
[Ad] Endereço:Urology Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, New York, United States of America.
[Ti] Título:Benign and tumor parenchyma metabolomic profiles affect compensatory renal growth in renal cell carcinoma surgical patients.
[So] Source:PLoS One;12(7):e0180350, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AND OBJECTIVES: Pre-operative kidney volume is an independent predictor of glomerular filtration rate in renal cell carcinoma patients. Compensatory renal growth (CRG) can ensue prior to nephrectomy in parallel to tumor growth and benign parenchyma loss. We aimed to test whether renal metabolite abundances significantly associate with CRG, suggesting a causative relationship. DESIGN, SETTING, PARTICIPANTS, AND MEASUREMENTS: Tissue metabolomics data from 49 patients, with a median age of 60 years, were previously collected and the pre-operative fold-change of their contra to ipsi-lateral benign kidney volume served as a surrogate for their CRG. Contra-lateral kidney volume fold-change within a 3.3 +/- 2.1 years follow-up interval was used as a surrogate for long-term CRG. Using a multivariable statistical model, we identified metabolites whose abundances significantly associate with CRG. RESULTS: Our analysis found 13 metabolites in the benign (e.g. L-urobilin, Variable Influence in Projection, VIP, score = 3.02, adjusted p = 0.017) and 163 metabolites in the malignant (e.g. 3-indoxyl-sulfate, VIP score = 1.3, adjusted p = 0.044) tissues that significantly associate with CRG. Benign/tumor fold change in metabolite abundances revealed three additional metabolites with that significantly positively associate with CRG (e.g. p-cresol sulfate, VIP score = 2.945, adjusted p = 0.033). At the pathway level, we show that fatty-acid oxidation is highly enriched with metabolites whose benign tissue abundances strongly positively associate with CRG, both pre-operatively and long term, whereas in the tumor tissue significant enrichment of dipeptides and benzoate (positive association), glycolysis/gluconeogenesis, lysolipid and nucleotide sugar pentose (negative associations) sub-pathways, were observed. CONCLUSION: These data suggest that specific biological processes in the benign as well as in the tumor parenchyma strongly influence compensatory renal growth.
[Mh] Termos MeSH primário: Carcinoma de Células Renais/metabolismo
Neoplasias Renais/metabolismo
Rim/metabolismo
Tecido Parenquimatoso/metabolismo
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Carcinoma de Células Renais/patologia
Carcinoma de Células Renais/cirurgia
Feminino
Seres Humanos
Rim/patologia
Rim/cirurgia
Neoplasias Renais/patologia
Neoplasias Renais/cirurgia
Masculino
Metabolômica
Meia-Idade
Nefrectomia
Tecido Parenquimatoso/patologia
Carga Tumoral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170927
[Lr] Data última revisão:
170927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170721
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0180350


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[PMID]:28708462
[Au] Autor:Wu J; Li B; Sun X; Cao G; Rubin DL; Napel S; Ikeda DM; Kurian AW; Li R
[Ad] Endereço:From the Department of Radiation Oncology (J.W., B.L., R.L.), Department of Radiology (D.L.R., S.N., D.M.I.), Department of Biomedical Data Science and Medicine (Biomedical Informatics Research) (D.L.R.), Department of Medicine (A.W.K.), Department of Health Research and Policy (A.W.K.), and Stanfor
[Ti] Título:Heterogeneous Enhancement Patterns of Tumor-adjacent Parenchyma at MR Imaging Are Associated with Dysregulated Signaling Pathways and Poor Survival in Breast Cancer.
[So] Source:Radiology;285(2):401-413, 2017 Nov.
[Is] ISSN:1527-1315
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Purpose To identify the molecular basis of quantitative imaging characteristics of tumor-adjacent parenchyma at dynamic contrast material-enhanced magnetic resonance (MR) imaging and to evaluate their prognostic value in breast cancer. Materials and Methods In this institutional review board-approved, HIPAA-compliant study, 10 quantitative imaging features depicting tumor-adjacent parenchymal enhancement patterns were extracted and screened for prognostic features in a discovery cohort of 60 patients. By using data from The Cancer Genome Atlas (TCGA), a radiogenomic map for the tumor-adjacent parenchymal tissue was created and molecular pathways associated with prognostic parenchymal imaging features were identified. Furthermore, a multigene signature of the parenchymal imaging feature was built in a training cohort (n = 126), and its prognostic relevance was evaluated in two independent cohorts (n = 879 and 159). Results One image feature measuring heterogeneity (ie, information measure of correlation) was significantly associated with prognosis (false-discovery rate < 0.1), and at a cutoff of 0.57 stratified patients into two groups with different recurrence-free survival rates (log-rank P = .024). The tumor necrosis factor signaling pathway was identified as the top enriched pathway (hypergeometric P < .0001) among genes associated with the image feature. A 73-gene signature based on the tumor profiles in TCGA achieved good association with the tumor-adjacent parenchymal image feature (R = 0.873), which stratified patients into groups regarding recurrence-free survival (log-rank P = .029) and overall survival (log-rank P = .042) in an independent TCGA cohort. The prognostic value was confirmed in another independent cohort (Gene Expression Omnibus GSE 1456), with log-rank P = .00058 for recurrence-free survival and log-rank P = .0026 for overall survival. Conclusion Heterogeneous enhancement patterns of tumor-adjacent parenchyma at MR imaging are associated with the tumor necrosis signaling pathway and poor survival in breast cancer. RSNA, 2017 Online supplemental material is available for this article.
[Mh] Termos MeSH primário: Neoplasias da Mama/diagnóstico por imagem
Neoplasias da Mama/mortalidade
Interpretação de Imagem Assistida por Computador
Imagem por Ressonância Magnética
Tecido Parenquimatoso/diagnóstico por imagem
[Mh] Termos MeSH secundário: Adulto
Idoso
Biomarcadores Tumorais/análise
Biomarcadores Tumorais/genética
Biomarcadores Tumorais/metabolismo
Neoplasias da Mama/química
Neoplasias da Mama/genética
Feminino
Perfilação da Expressão Gênica
Genômica
Seres Humanos
Estimativa de Kaplan-Meier
Meia-Idade
Imagem Molecular
Tecido Parenquimatoso/química
Prognóstico
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171121
[Lr] Data última revisão:
171121
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170715
[St] Status:MEDLINE
[do] DOI:10.1148/radiol.2017162823



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