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[PMID]:29391275
[Au] Autor:Yang X; Wang J; Bing G; Bie P; De Y; Lyu Y; Wu Q
[Ad] Endereço:Key Laboratory of Animal Epidemiology and Zoonosis of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
[Ti] Título:Ortholog-based screening and identification of genes related to intracellular survival.
[So] Source:Gene;651:134-142, 2018 Apr 20.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Bioinformatics and comparative genomics analysis methods were used to predict unknown pathogen genes based on homology with identified or functionally clustered genes. In this study, the genes of common pathogens were analyzed to screen and identify genes associated with intracellular survival through sequence similarity, phylogenetic tree analysis and the λ-Red recombination system test method. The total 38,952 protein-coding genes of common pathogens were divided into 19,775 clusters. As demonstrated through a COG analysis, information storage and processing genes might play an important role intracellular survival. Only 19 clusters were present in facultative intracellular pathogens, and not all were present in extracellular pathogens. Construction of a phylogenetic tree selected 18 of these 19 clusters. Comparisons with the DEG database and previous research revealed that seven other clusters are considered essential gene clusters and that seven other clusters are associated with intracellular survival. Moreover, this study confirmed that clusters screened by orthologs with similar function could be replaced with an approved uvrY gene and its orthologs, and the results revealed that the usg gene is associated with intracellular survival. The study improves the current understanding of intracellular pathogens characteristics and allows further exploration of the intracellular survival-related gene modules in these pathogens.
[Mh] Termos MeSH primário: Bactérias/genética
Fenômenos Fisiológicos Bacterianos
Células/microbiologia
Genes Bacterianos
[Mh] Termos MeSH secundário: Animais
Proteínas de Bactérias/genética
Proteínas de Bactérias/fisiologia
Células Cultivadas
Genes Essenciais
Interações Hospedeiro-Patógeno
Camundongos
Família Multigênica
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180203
[St] Status:MEDLINE


  2 / 7137 MEDLINE  
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[PMID]:27771543
[Au] Autor:Qin S; Zhou HX
[Ad] Endereço:Department of Physics and Institute of Molecular Biophysics, Florida State University, Tallahassee, FL 32306, USA.
[Ti] Título:Protein folding, binding, and droplet formation in cell-like conditions.
[So] Source:Curr Opin Struct Biol;43:28-37, 2017 04.
[Is] ISSN:1879-033X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The many bystander macromolecules in the crowded cellular environments present both steric repulsion and weak attraction to proteins undergoing folding or binding and hence impact the thermodynamic and kinetic properties of these processes. The weak but nonrandom binding with bystander macromolecules may facilitate subcellular localization and biological function. Weak binding also leads to the emergence of a protein-rich droplet phase, which has been implicated in regulating a variety of cellular functions. All these important problems can now be addressed by realistic modeling of intermolecular interactions. Configurational sampling of concentrated protein solutions is an ongoing challenge.
[Mh] Termos MeSH primário: Dobramento de Proteína
Proteínas/química
Proteínas/metabolismo
[Mh] Termos MeSH secundário: Células/metabolismo
Ligação Proteica
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Proteins)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180224
[Lr] Data última revisão:
180224
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


  3 / 7137 MEDLINE  
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[PMID]:28743585
[Au] Autor:Torday JS; Miller WB
[Ad] Endereço:Department of Pediatrics, Harbor-UCLA Medical Center, Torrance, CA 90502, USA. Electronic address: jtorday@labiomed.org.
[Ti] Título:The resolution of ambiguity as the basis for life: A cellular bridge between Western reductionism and Eastern holism.
[So] Source:Prog Biophys Mol Biol;131:288-297, 2017 Dec.
[Is] ISSN:1873-1732
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Boundary conditions enable cellular life through negentropy, chemiosmosis, and homeostasis as identifiable First Principles of Physiology. Self-referential awareness of status arises from this organized state to sustain homeostatic imperatives. Preferred homeostatic status is dependent upon the appraisal of information and its communication. However, among living entities, sources of information and their dissemination are always imprecise. Consequently, living systems exist within an innate state of ambiguity. It is presented that cellular life and evolutionary development are a self-organizing cellular response to uncertainty in iterative conformity with its basal initiating parameters. Viewing the life circumstance in this manner permits a reasoned unification between Western rational reductionism and Eastern holism.
[Mh] Termos MeSH primário: Biologia Celular
Células/citologia
Filosofia
Ocidente
[Mh] Termos MeSH secundário: Animais
Comunicação Celular
Células/metabolismo
Epigênese Genética
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180126
[Lr] Data última revisão:
180126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE


  4 / 7137 MEDLINE  
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[PMID]:29172499
[Au] Autor:Je HJ; Kim ES; Lee JS; Lee HG
[Ad] Endereço:Department of Food and Nutrition, Hanyang University , 222, Wangsimni-ro, Seoungdong-gu, Seoul 04763, Republic of Korea.
[Ti] Título:Release Properties and Cellular Uptake in Caco-2 Cells of Size-Controlled Chitosan Nanoparticles.
[So] Source:J Agric Food Chem;65(50):10899-10906, 2017 Dec 20.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The influences of particle size on the physicochemical, release, and cellular uptake properties of chitosan nanoparticles (CSNPs) were investigated. Ionotropic CSNPs of different sizes (200-1000 nm) loaded with two model core materials (resveratrol or coumarin-6) were prepared using tripolyphosphate and carrageenan as cross-linkers. With an increase of particle size, zeta potential (34.6 ± 0.5 to 51.1 ± 0.9) and entrapment efficiency (14.9 ± 1.4 to 40.9 ± 1.9) of the CSNPs were significantly (p < 0.05) increased and release rates were decreased. However, Caco-2 cellular uptake of CSNPs were significantly increased from 3.70 ± 0.03 to 5.24 ± 0.20 with an increase of particle size from 200 to 600 nm, whereas those significantly decreased from 5.24 ± 0.20 to 4.55 ± 0.2 for particles larger than 600 nm in transwell assay. Moreover, much the same uptake patterns were also observed in confocal microscopy and flow cytometry. Investigation of cellular uptake of CSNPs revealed positive correlations between ZP and EE and indicated the effects of complex factors of nanoparticles other than size. These results provide a better understanding of CSNPs absorption and raises the possibility of controlling alternative nanoparticle properties to enhance bioavailability.
[Mh] Termos MeSH primário: Células/metabolismo
Quitosana/química
Cumarínicos/metabolismo
Portadores de Fármacos/química
Nanopartículas/química
Estilbenos/metabolismo
Tiazóis/metabolismo
[Mh] Termos MeSH secundário: Transporte Biológico
Células CACO-2
Química Farmacêutica
Quitosana/metabolismo
Cumarínicos/química
Portadores de Fármacos/metabolismo
Seres Humanos
Nanopartículas/metabolismo
Tamanho da Partícula
Estilbenos/química
Tiazóis/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coumarins); 0 (Drug Carriers); 0 (Stilbenes); 0 (Thiazoles); 0 (coumarin 6); 9012-76-4 (Chitosan); Q369O8926L (resveratrol)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180110
[Lr] Data última revisão:
180110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b03627


  5 / 7137 MEDLINE  
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[PMID]:29045427
[Au] Autor:Gabel M; Regoes RR; Graw F
[Ad] Endereço:Center for Modelling and Simulation in the Biosciences, BioQuant-Center, Heidelberg University, 69120 Heidelberg, Germany.
[Ti] Título:More or less-On the influence of labelling strategies to infer cell population dynamics.
[So] Source:PLoS One;12(10):e0185523, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The adoptive transfer of labelled cell populations has been an essential tool to determine and quantify cellular dynamics. The experimental methods to label and track cells over time range from fluorescent dyes over congenic markers towards single-cell labelling techniques, such as genetic barcodes. While these methods have been widely used to quantify cell differentiation and division dynamics, the extent to which the applied labelling strategy actually affects the quantification of the dynamics has not been determined so far. This is especially important in situations where measurements can only be obtained at a single time point, as e.g. due to organ harvest. To this end, we studied the appropriateness of various labelling strategies as characterised by the number of different labels and the initial number of cells per label to quantify cellular dynamics. We simulated adoptive transfer experiments in systems of various complexity that assumed either homoeostatic cellular turnover or cell expansion dynamics involving various steps of cell differentiation and proliferation. Re-sampling cells at a single time point, we determined the ability of different labelling strategies to recover the underlying kinetics. Our results indicate that cell transition and expansion rates are differently affected by experimental shortcomings, such as loss of cells during transfer or sampling, dependent on the labelling strategy used. Furthermore, uniformly distributed labels in the transferred population generally lead to more robust and less biased results than non-equal label sizes. In addition, our analysis indicates that certain labelling approaches incorporate a systematic bias for the identification of complex cell expansion dynamics.
[Mh] Termos MeSH primário: Células/citologia
Coloração e Rotulagem/métodos
[Mh] Termos MeSH secundário: Diferenciação Celular
Proliferação Celular
Homeostase
Modelos Biológicos
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171019
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185523


  6 / 7137 MEDLINE  
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[PMID]:28888322
[Au] Autor:Blenis J
[Ad] Endereço:Meyer Cancer Center and Department of Pharmacology, Weill Cornell Medicine, 413 East 69(th) Street, New York, NY 10021, USA. Electronic address: jblenis@med.cornell.edu.
[Ti] Título:TOR, the Gateway to Cellular Metabolism, Cell Growth, and Disease.
[So] Source:Cell;171(1):10-13, 2017 Sep 21.
[Is] ISSN:1097-4172
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Michael N. Hall is this year's recipient of the Lasker Basic Medical Research Award for the identification of the target of rapamycin, TOR. TOR is a master regulator of the cell's growth and metabolic state, and its dysregulation contributes to a variety of diseases, including diabetes, obesity, neurodegenerative disorders, aging, and cancer, making the TOR pathway an attractive therapeutic target.
[Mh] Termos MeSH primário: Distinções e Prêmios
Células/metabolismo
Fisiologia/história
Transdução de Sinais
Serina-Treonina Quinases TOR/fisiologia
[Mh] Termos MeSH secundário: Animais
Antifúngicos/química
Antifúngicos/isolamento & purificação
Antifúngicos/uso terapêutico
História do Século XX
Seres Humanos
Neoplasias/tratamento farmacológico
Sirolimo/química
Sirolimo/isolamento & purificação
Sirolimo/uso terapêutico
Suíça
[Pt] Tipo de publicação:BIOGRAPHY; HISTORICAL ARTICLE; JOURNAL ARTICLE
[Ps] Nome de pessoa como assunto:Hall MN
[Nm] Nome de substância:
0 (Antifungal Agents); EC 2.7.1.1 (TOR Serine-Threonine Kinases); W36ZG6FT64 (Sirolimus)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170911
[St] Status:MEDLINE


  7 / 7137 MEDLINE  
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[PMID]:28768315
[Au] Autor:Abbasi J
[Ti] Título:An International Human Cell Atlas Consortium Takes Shape.
[So] Source:JAMA;318(8):685-686, 2017 Aug 22.
[Is] ISSN:1538-3598
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Atlas como Assunto
Células/citologia
[Mh] Termos MeSH secundário: Anatomia Artística
Animais
Células/ultraestrutura
Seres Humanos
Internacionalidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170803
[St] Status:MEDLINE
[do] DOI:10.1001/jama.2017.5640


  8 / 7137 MEDLINE  
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[PMID]:28750084
[Au] Autor:Jin W; Penington CJ; McCue SW; Simpson MJ
[Ad] Endereço:School of Mathematical Sciences, Queensland University of Technology (QUT) Brisbane, Queensland 4000, Australia.
[Ti] Título:A computational modelling framework to quantify the effects of passaging cell lines.
[So] Source:PLoS One;12(7):e0181941, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In vitro cell culture is routinely used to grow and supply a sufficiently large number of cells for various types of cell biology experiments. Previous experimental studies report that cell characteristics evolve as the passage number increases, and various cell lines can behave differently at high passage numbers. To provide insight into the putative mechanisms that might give rise to these differences, we perform in silico experiments using a random walk model to mimic the in vitro cell culture process. Our results show that it is possible for the average proliferation rate to either increase or decrease as the passaging process takes place, and this is due to a competition between the initial heterogeneity and the degree to which passaging damages the cells. We also simulate a suite of scratch assays with cells from near-homogeneous and heterogeneous cell lines, at both high and low passage numbers. Although it is common in the literature to report experimental results without disclosing the passage number, our results show that we obtain significantly different closure rates when performing in silico scratch assays using cells with different passage numbers. Therefore, we suggest that the passage number should always be reported to ensure that the experiment is as reproducible as possible. Furthermore, our modelling also suggests some avenues for further experimental examination that could be used to validate or refine our simulation results.
[Mh] Termos MeSH primário: Células/citologia
Simulação por Computador
[Mh] Termos MeSH secundário: Contagem de Células
Linhagem Celular
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0181941


  9 / 7137 MEDLINE  
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[PMID]:28718621
[Au] Autor:Mehta S; Zhang J
[Ad] Endereço:Department of Pharmacology, University of California, San Diego , La Jolla, California 92093, United States.
[Ti] Título:Illuminating the Cell's Biochemical Activity Architecture.
[So] Source:Biochemistry;56(39):5210-5213, 2017 Oct 03.
[Is] ISSN:1520-4995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:All cellular behaviors arise through the coordinated actions of numerous intracellular biochemical pathways. Over the past 20 years, efforts to probe intracellular biochemical processes have undergone a fundamental transformation brought about by advances in fluorescence imaging, such as the development of genetically encoded fluorescent reporters and new imaging technologies; the impact of these approaches on our understanding of the molecular underpinnings of biological function cannot be understated. In particular, the ability to obtain information on the spatiotemporal regulation of biochemical processes unfolding in real time in the native context of a living cell has crystallized the view, long a matter of speculation, that cells achieve specific biological outcomes through the imposition of spatial control over the distribution of various biomolecules, and their associated biochemical activities, within the cellular environment. Indeed, the compartmentalization of biochemical activities by cells is now known to be pervasive and to span a multitude of spatial scales, from the length of a cell to just a few enzymes. In this Perspective, part of this special issue on "Seeing into cells", we highlight several recent imaging studies that provide detailed insights into not just where molecules are but where molecules are active within cells, offering a glimpse into the emerging view of biochemical activity architecture as a complement to the physical architecture of a cell.
[Mh] Termos MeSH primário: Células/metabolismo
Imagem Molecular/métodos
[Mh] Termos MeSH secundário: Animais
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170719
[St] Status:MEDLINE
[do] DOI:10.1021/acs.biochem.7b00561


  10 / 7137 MEDLINE  
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[PMID]:28714480
[Au] Autor:Técher H; Koundrioukoff S; Nicolas A; Debatisse M
[Ad] Endereço:IFOM, the FIRC institute of Molecular Oncology, DNA metabolism laboratory, Via Adamello 16, 20139 Milan, Italy.
[Ti] Título:The impact of replication stress on replication dynamics and DNA damage in vertebrate cells.
[So] Source:Nat Rev Genet;18(9):535-550, 2017 Sep.
[Is] ISSN:1471-0064
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The interplay between replication stress and the S phase checkpoint is a key determinant of genome maintenance, and has a major impact on human diseases, notably, tumour initiation and progression. Recent studies have yielded insights into sequence-dependent and sequence-independent sources of endogenous replication stress. These stresses result in nuclease-induced DNA damage, checkpoint activation and genome-wide replication fork slowing. Several hypotheses have been proposed to account for the mechanisms involved in this complex response. Recent results have shown that the slowing of the replication forks most commonly results from DNA precursor starvation. By concomitantly increasing the density of replication initiation, the cell elicits an efficient compensatory strategy to avoid mitotic anomalies and the inheritance of damage over cell generations.
[Mh] Termos MeSH primário: Dano ao DNA
Replicação do DNA
[Mh] Termos MeSH secundário: Animais
Ciclo Celular
Células/metabolismo
Desoxirribonucleotídeos/metabolismo
Seres Humanos
Conformação de Ácido Nucleico
Transcrição Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Deoxyribonucleotides)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170818
[Lr] Data última revisão:
170818
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170718
[St] Status:MEDLINE
[do] DOI:10.1038/nrg.2017.46



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