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[PMID]:29431945
[Au] Autor:Sinitskaya TA; Malinovskaya NN
[Ti] Título:[Toxicological-hygienic justification of the acceptable daily intake of acetamiprid].
[So] Source:Gig Sanit;95(11):1055-8, 2016.
[Is] ISSN:0016-9900
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Neonicotinoids are currently meaningful component of rotation schemes of insecticides of selective action in the system of integrated pest control, which have agricultural importance in many countries. The research results of the biological impact of acetamiprid (neonicotinoids) on the body of laboratory animals are given in the article. The study showed that the explored active substance is related to the moderately hazardous compounds (hazard category 3) in case of one-time per oral penetration. Acetamiprid has polytropic action in the case of chronic (12 months) oral entering the body of laboratory animals, it gives rise changes in functionality of the central nervous system, blood system, liver functioning. On the base of alterations of the studied indices there were established both the no-effect dose level (NOEL) and acceptable daily intake of acetamiprid for humans.
[Mh] Termos MeSH primário: Células Sanguíneas/efeitos dos fármacos
Sistema Nervoso Central/efeitos dos fármacos
Neonicotinoides
Envenenamento
[Mh] Termos MeSH secundário: Administração Oral
Animais
Comportamento Animal/efeitos dos fármacos
Modelos Animais de Doenças
Testes Hematológicos/métodos
Inseticidas/farmacologia
Inseticidas/toxicidade
Testes de Função Hepática/métodos
Concentração Máxima Permitida
Neonicotinoides/farmacologia
Neonicotinoides/toxicidade
Nível de Efeito Adverso não Observado
Órgãos em Risco
Envenenamento/sangue
Envenenamento/diagnóstico
Envenenamento/etiologia
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insecticides); 0 (Neonicotinoids); 5HL5N372P0 (acetamiprid)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180213
[St] Status:MEDLINE


  2 / 10108 MEDLINE  
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[PMID]:29351567
[Au] Autor:Ghandhi SA; Turner HC; Shuryak I; Dugan GO; Bourland JD; Olson JD; Tooze JA; Morton SR; Batinic-Haberle I; Cline JM; Amundson SA
[Ad] Endereço:Center for Radiological Research, Columbia University Medical Center, New York, New York, United States of America.
[Ti] Título:Whole thorax irradiation of non-human primates induces persistent nuclear damage and gene expression changes in peripheral blood cells.
[So] Source:PLoS One;13(1):e0191402, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We investigated the cytogenetic and gene expression responses of peripheral blood cells of non-human primates (NHP, Macaca mulatta) that were whole-thorax irradiated with a single dose of 10 Gy. In this model, partial irradiation of NHPs in the thoracic region (Whole Thorax Lung Irradiation, WTLI) allows the study of late radiation-induced lung injury, while avoiding acute radiation syndromes related to hematopoietic and gastrointestinal injury. A transient drop in circulating lymphocytes and platelets was seen by 9 days, followed by elevations in respiratory rate, circulating neutrophils, lymphocytes, and monocytes at 60-100 days, corresponding to computed tomography (CT) and histologic evidence of pneumonitis, and elective euthanasia of four animals. To evaluate long-term DNA damage in NHP peripheral blood lymphocytes after 10 Gy WTLI, we used the cytokinesis-block micronucleus (CBMN) assay to measure chromosomal aberrations as post-mitotic micronuclei in blood samples collected up to 8 months after irradiation. Regression analysis showed significant induction of micronuclei in NHP blood cells that persisted with a gradual decline over the 8-month study period, suggesting long-term DNA damage in blood lymphocytes after WTLI. We also report transcriptomic changes in blood up to 30 days after WTLI. We isolated total RNA from peripheral blood at 3 days before and then at 2, 5 and 30 days after irradiation. We identified 1187 transcripts that were significantly changed across the 30-day time course. From changes in gene expression, we identified biological processes related to immune responses, which persisted across the 30-day study. Response to oxygen-containing compounds and bacteria were implicated by gene-expression changes at the earliest day 2 and latest, day 30 time-points. Gene expression changes suggest a persistent altered state of the immune system, specifically response to infection, for at least a month after WTLI.
[Mh] Termos MeSH primário: Células Sanguíneas/metabolismo
Células Sanguíneas/efeitos da radiação
Dano ao DNA
Expressão Gênica/efeitos da radiação
[Mh] Termos MeSH secundário: Animais
Contagem de Células Sanguíneas
Aberrações Cromossômicas
Relação Dose-Resposta à Radiação
Ontologia Genética
Seres Humanos
Lesão Pulmonar/sangue
Lesão Pulmonar/etiologia
Lesão Pulmonar/genética
Macaca mulatta/sangue
Macaca mulatta/genética
Masculino
Testes para Micronúcleos
Lesões Experimentais por Radiação/sangue
Lesões Experimentais por Radiação/genética
Tórax/efeitos da radiação
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180120
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191402


  3 / 10108 MEDLINE  
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[PMID]:29025669
[Au] Autor:Huang N; Lin J; Li S; Deng Y; Kong S; Hong P; Yang P; Liao M; Hu Z
[Ad] Endereço:Faculty of Chemistry and Environment Science, Guangdong Ocean University, Zhanjiang 524088, PR China. Electronic address: huangn88@126.com.
[Ti] Título:Preparation and evaluation of squid ink polysaccharide-chitosan as a wound-healing sponge.
[So] Source:Mater Sci Eng C Mater Biol Appl;82:354-362, 2018 Jan 01.
[Is] ISSN:1873-0191
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A new type of wound healing agent was developed using two marine biomaterials (squid ink polysaccharide and chitosan) as carriers and calcium chloride as an initiator for coagulation. Based on central composite design-response surface methodology, comprehensive evaluation of appearance quality for composite sponges and water absorbency were used as evaluation indices to identify the optimized preparation conditions and further evaluate the performance of the squid ink polysaccharide-chitosan sponge (SIP-CS). The optimized formulation of SIP-CS was as follows: chitosan concentration, 2.29%; squid ink polysaccharide concentration, 0.55%; and calcium chloride concentration, 2.82%, at a volume ratio of 15:5:2. SIP-CS was conducive to sticking on the wound, characterized by the spongy property, strong absorptivity, and tackiness. Rabbit ear arterial, hepatic, and femoral artery hemorrhage experiments indicated that, compared with chitosan dressings and absorbable gelatin, the hemostatic times were shorter and the bleeding volume was smaller. Furthermore, SIP-CS absorbed a large amount of hemocytes, leading to rapid hemostasis. The healing areas and wound pathological sections in scalded New Zealand rabbits indicated that SIP-CS promoted wound healing more rapidly than chitosan and better than commercially available burn cream. Thus, SIP-CS is a good wound healing agent for rapid hemostasis, promoting burn/scalded skin healing, and protecting from wound infection.
[Mh] Termos MeSH primário: Materiais Biocompatíveis/química
Quitosana/química
Decapodiformes/metabolismo
Tinta
Polissacarídeos/química
[Mh] Termos MeSH secundário: Adsorção
Animais
Artérias/lesões
Bandagens
Materiais Biocompatíveis/farmacologia
Células Sanguíneas/citologia
Células Sanguíneas/efeitos dos fármacos
Células Sanguíneas/metabolismo
Hemorragia/prevenção & controle
Microscopia Eletrônica de Varredura
Coelhos
Cicatrização/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biocompatible Materials); 0 (Polysaccharides); 9012-76-4 (Chitosan)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171014
[St] Status:MEDLINE


  4 / 10108 MEDLINE  
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[PMID]:29025666
[Au] Autor:Horakova J; Mikes P; Saman A; Svarcova T; Jencova V; Suchy T; Heczkova B; Jakubkova S; Jirousova J; Prochazkova R
[Ad] Endereço:Technical University of Liberec, Faculty of Textile, Department of Nonwovens and Nanofibrous Materials, Studentska 2, 461 17 Liberec, Czech Republic. Electronic address: jana.horakova@tul.cz.
[Ti] Título:Comprehensive assessment of electrospun scaffolds hemocompatibility.
[So] Source:Mater Sci Eng C Mater Biol Appl;82:330-335, 2018 Jan 01.
[Is] ISSN:1873-0191
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Biodegradable polyesters, namely polycaprolactone (PCL) and copolymer of polylactide and polycaprolactone (PLCL) were electrospun into various fibrous structures and their hemocompatibility was evaluated in vitro. Firstly, hemolytic effect was evaluated upon incubation with diluted whole blood. The results showed that the degree of hemolysis depended on chemical composition and fibrous morphology. Electrospun polycaprolactone induced slight degree of hemolysis depending on its molecular weight and fibrous morphology; copolymer PLCL did not cause detectable hemolysis. The influence of coagulation pathways was examined by measurement of coagulation times. It was showed that intrinsic coagulation pathway assessed by activated partial thromboplastin time (APTT) was moderately accelerated after incubation with PCL and prolonged after incubation with copolymer PLCL. Extrinsic activation of coagulation tested by prothrombin time (PT) was slightly accelerated after incubation with all tested electrospun samples. Thrombogenicity assessment of fibrous samples revealed high thrombogenic properties of fibrous materials that was comparable to high degree of collagen thrombogenicity. The level of platelet activation was dependent on chemical composition and surface morphology of tested materials.
[Mh] Termos MeSH primário: Materiais Biocompatíveis/química
Polímeros/química
[Mh] Termos MeSH secundário: Materiais Biocompatíveis/síntese química
Materiais Biocompatíveis/farmacologia
Células Sanguíneas/citologia
Células Sanguíneas/efeitos dos fármacos
Células Sanguíneas/metabolismo
Colágeno/química
Hemólise/efeitos dos fármacos
Seres Humanos
Microscopia Eletrônica de Varredura
Tempo de Tromboplastina Parcial
Poliésteres/química
Polímeros/síntese química
Tempo de Protrombina
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biocompatible Materials); 0 (Polyesters); 0 (Polymers); 24980-41-4 (polycaprolactone); 459TN2L5F5 (poly(lactide)); 9007-34-5 (Collagen)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171014
[St] Status:MEDLINE


  5 / 10108 MEDLINE  
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[PMID]:28745632
[Au] Autor:Li Y; Hai Y; Chen J; Liu T
[Ad] Endereço:Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences.
[Ti] Título:Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells.
[So] Source:J Vis Exp;(125), 2017 07 18.
[Is] ISSN:1940-087X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this study, we used peripheral blood cells (PBCs) as seed cells to produce chondrocytes via induced pluripotent stem cells (iPSCs) in an integration-free method. Following embryoid body (EB) formation and fibroblastic cell expansion, the iPSCs are induced for chondrogenic differentiation for 21 days under serum-free and xeno-free conditions. After chondrocyte induction, the phenotypes of the cells are evaluated by morphological, immunohistochemical, and biochemical analyses, as well as by the quantitative real-time PCR examination of chondrogenic differentiation markers. The chondrogenic pellets show positive alcian blue and toluidine blue staining. The immunohistochemistry of collagen II and X staining is also positive. The sulfated glycosaminoglycan (sGAG) content and the chondrogenic differentiation markers COLLAGEN 2 (COL2), COLLAGEN 10 (COL10), SOX9, and AGGRECAN are significantly upregulated in chondrogenic pellets compared to hiPSCs and fibroblastic cells. These results suggest that PBCs can be used as seed cells to generate iPSCs for cartilage repair, which is patient-specific and cost-effective.
[Mh] Termos MeSH primário: Células Sanguíneas/citologia
Condrócitos/citologia
Células-Tronco Pluripotentes Induzidas/citologia
[Mh] Termos MeSH secundário: Agrecanas/genética
Agrecanas/metabolismo
Diferenciação Celular
Células Cultivadas
Condrócitos/metabolismo
Condrogênese
Colágeno Tipo II/genética
Colágeno Tipo II/metabolismo
Corpos Embrioides/citologia
Corpos Embrioides/patologia
Seres Humanos
Imuno-Histoquímica
Células-Tronco Pluripotentes Induzidas/metabolismo
Fatores de Transcrição SOX9/genética
Fatores de Transcrição SOX9/metabolismo
Regulação para Cima
Gravação em Vídeo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aggrecans); 0 (Collagen Type II); 0 (SOX9 Transcription Factor)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180130
[Lr] Data última revisão:
180130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.3791/55722


  6 / 10108 MEDLINE  
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[PMID]:28452249
[Au] Autor:Liu C; Liu J; Hao Y; Gu Y; Yang Z; Li H; Li R
[Ad] Endereço:a Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, Chongqing Engineering Research Center for Nanomedicine , College of Preventive Medicine, Third Military Medical University , Chongqing , China.
[Ti] Título:6,7,3',4'-Tetrahydroxyisoflavone improves the survival of whole-body-irradiated mice via restoration of hematopoietic function.
[So] Source:Int J Radiat Biol;93(8):793-802, 2017 08.
[Is] ISSN:1362-3095
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: 6,7,3',4'-tetrahydroxyisoflavone (T3) is a novel chemically synthesized compound reported in our previous study. This study was designed to explore the radioprotective effect of T3, and if so, its potential mechanisms. MATERIALS AND METHODS: KunMing mice were exposed to various doses of γ irradiation (60 Co) after being treated with dimethyl sulfoxide (DMSO) or T3. Briefly, survival rate, dose reducing factor (DRF), body weight change (%), spleen index (SI) and thymus index (TI) of irradiated mice with or without different doses of T3 treatment were evaluated routinely. The hematopoietic function of bone marrow was emphatically investigated. In vitro experiments were performed to observe the protective effect of T3 on irradiated human lymphocyte AHH-1 cells by cell viability or flow cytometry (FCM) assays. RESULTS: A single dose of subcutaneous administration of T3 significantly improved the survival rate, and enhanced the restoration of hematopoietic function in irradiated mice. T3 also decreased the apoptosis of irradiated AHH-1 cells in vitro. CONCLUSIONS: T3 protected mice against lethal γ irradiation-induced injury probably through the restoration of hematopoietic function. This implied that T3 could be further developed as a radioprotector.
[Mh] Termos MeSH primário: Raios gama/efeitos adversos
Hematopoese/efeitos dos fármacos
Hematopoese/efeitos da radiação
Isoflavonas/farmacologia
Protetores contra Radiação/farmacologia
Irradiação Corporal Total/efeitos adversos
[Mh] Termos MeSH secundário: Animais
Apoptose/efeitos dos fármacos
Apoptose/efeitos da radiação
Células Sanguíneas/citologia
Células Sanguíneas/efeitos dos fármacos
Células Sanguíneas/efeitos da radiação
Peso Corporal/efeitos dos fármacos
Peso Corporal/efeitos da radiação
Células da Medula Óssea/efeitos dos fármacos
Células da Medula Óssea/efeitos da radiação
Caspases/metabolismo
Sobrevivência Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos da radiação
Masculino
Camundongos
Análise de Sobrevida
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (6,7,3',4'-tetrahydroxyisoflavone); 0 (Isoflavones); 0 (Radiation-Protective Agents); EC 3.4.22.- (Caspases)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:180111
[Lr] Data última revisão:
180111
[Sb] Subgrupo de revista:IM; S
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1080/09553002.2017.1321808


  7 / 10108 MEDLINE  
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[PMID]:29237418
[Au] Autor:Cattaneo M; La Sala L; Rondinelli M; Errichiello E; Zuffardi O; Puca AA; Genovese S; Ceriello A
[Ad] Endereço:Cardiovascular Research Unit, IRCCS MultiMedica, Via G. Fantoli 16/15, 20138, Milan, Italy. monica.cattaneo@multimedica.it.
[Ti] Título:A donor splice site mutation in CISD2 generates multiple truncated, non-functional isoforms in Wolfram syndrome type 2 patients.
[So] Source:BMC Med Genet;18(1):147, 2017 12 13.
[Is] ISSN:1471-2350
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Mutations in the gene that encodes CDGSH iron sulfur domain 2 (CISD2) are causative of Wolfram syndrome type 2 (WFS2), a rare autosomal recessive neurodegenerative disorder mainly characterized by diabetes mellitus, optic atrophy, peptic ulcer bleeding and defective platelet aggregation. Four mutations in the CISD2 gene have been reported. Among these mutations, the homozygous c.103 + 1G > A substitution was identified in the donor splice site of intron 1 in two Italian sisters and was predicted to cause a exon 1 to be skipped. METHODS: Here, we employed molecular assays to characterize the c.103 + 1G > A mutation using the patient's peripheral blood mononuclear cells (PBMCs). 5'-RACE coupled with RT-PCR were used to analyse the effect of the c.103 + 1G > A mutation on mRNA splicing. Western blot analysis was used to analyse the consequences of the CISD2 mutation on the encoded protein. RESULTS: We demonstrated that the c.103 + 1G > A mutation functionally impaired mRNA splicing, producing multiple splice variants characterized by the whole or partial absence of exon 1, which introduced amino acid changes and a premature stop. The affected mRNAs resulted in either predicted targets for nonsense mRNA decay (NMD) or non-functional isoforms. CONCLUSIONS: We concluded that the c.103 + 1G > A mutation resulted in the loss of functional CISD2 protein in the two Italian WFS2 patients.
[Mh] Termos MeSH primário: Senilidade Prematura/genética
Perda Auditiva Neurossensorial/genética
Proteínas de Membrana/genética
Doenças Mitocondriais/genética
Mutação
Atrofia Óptica/genética
Sítios de Splice de RNA/genética
[Mh] Termos MeSH secundário: Sequência de Bases
Células Sanguíneas
Códon sem Sentido
Éxons/genética
Feminino
Seres Humanos
Íntrons/genética
Leucócitos Mononucleares
Proteínas de Membrana/química
Isoformas de Proteínas/genética
Sítios de Splice de RNA/fisiologia
Processamento de RNA
RNA Mensageiro/genética
Análise de Sequência
Deleção de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Codon, Nonsense); 0 (ERIS protein, human); 0 (Membrane Proteins); 0 (Protein Isoforms); 0 (RNA Splice Sites); 0 (RNA, Messenger)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171231
[Lr] Data última revisão:
171231
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171215
[St] Status:MEDLINE
[do] DOI:10.1186/s12881-017-0508-2


  8 / 10108 MEDLINE  
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[PMID]:29221581
[Au] Autor:Yancey SW; Keene ON; Albers FC; Ortega H; Bates S; Bleecker ER; Pavord I
[Ad] Endereço:Respiratory Therapeutic Area, GlaxoSmithKline, Research Triangle Park, NC. Electronic address: steve.w.yancey@gsk.com.
[Ti] Título:Biomarkers for severe eosinophilic asthma.
[So] Source:J Allergy Clin Immunol;140(6):1509-1518, 2017 Dec.
[Is] ISSN:1097-6825
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The last decade has seen the approval of several new biologics for the treatment of severe asthma-targeting specific endotypes and phenotypes. This review will examine how evidence generated from the mepolizumab clinical development program showed that blood eosinophil counts, rather than sputum or tissue eosinophil counts, evolved as a pharmacodynamic and predictive biomarker for the efficacy of treatment with mepolizumab in patients with severe eosinophilic asthma. Based on the available evidence and combined with clinical judgement, a baseline blood eosinophil threshold of 150 cells/µL or greater or a historical blood eosinophil threshold of 300 cells/µL or greater will allow selection of patients with severe eosinophilic asthma who are most likely to achieve clinically significant reductions in the rate of exacerbations with mepolizumab treatment.
[Mh] Termos MeSH primário: Asma/diagnóstico
Células Sanguíneas/patologia
Eosinófilos/patologia
Eosinofilia Pulmonar/diagnóstico
Escarro/citologia
[Mh] Termos MeSH secundário: Animais
Antiasmáticos/uso terapêutico
Anticorpos Monoclonais Humanizados/uso terapêutico
Asma/tratamento farmacológico
Biomarcadores Farmacológicos/metabolismo
Progressão da Doença
Seres Humanos
Interleucina-5/imunologia
Contagem de Leucócitos
Valor Preditivo dos Testes
Eosinofilia Pulmonar/tratamento farmacológico
Índice de Gravidade de Doença
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anti-Asthmatic Agents); 0 (Antibodies, Monoclonal, Humanized); 0 (Biomarkers, Pharmacological); 0 (Interleukin-5); 90Z2UF0E52 (mepolizumab)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171210
[St] Status:MEDLINE


  9 / 10108 MEDLINE  
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[PMID]:28957325
[Au] Autor:Dumeaux V; Fjukstad B; Fjosne HE; Frantzen JO; Holmen MM; Rodegerdts E; Schlichting E; Børresen-Dale AL; Bongo LA; Lund E; Hallett M
[Ad] Endereço:Department of Biology, Concordia University, Montreal, QC, Canada.
[Ti] Título:Interactions between the tumor and the blood systemic response of breast cancer patients.
[So] Source:PLoS Comput Biol;13(9):e1005680, 2017 Sep.
[Is] ISSN:1553-7358
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Although systemic immunity is critical to the process of tumor rejection, cancer research has largely focused on immune cells in the tumor microenvironment. To understand molecular changes in the patient systemic response (SR) to the presence of BC, we profiled RNA in blood and matched tumor from 173 patients. We designed a system (MIxT, Matched Interactions Across Tissues) to systematically explore and link molecular processes expressed in each tissue. MIxT confirmed that processes active in the patient SR are especially relevant to BC immunogenicity. The nature of interactions across tissues (i.e. which biological processes are associated and their patterns of expression) varies highly with tumor subtype. For example, aspects of the immune SR are underexpressed proportionally to the level of expression of defined molecular processes specific to basal tumors. The catalog of subtype-specific interactions across tissues from BC patients provides promising new ways to tackle or monitor the disease by exploiting the patient SR.
[Mh] Termos MeSH primário: Células Sanguíneas/fisiologia
Neoplasias da Mama/fisiopatologia
Microambiente Celular/fisiologia
Microambiente Tumoral/fisiologia
[Mh] Termos MeSH secundário: Biomarcadores Tumorais/análise
Biomarcadores Tumorais/genética
Biomarcadores Tumorais/metabolismo
Estudos de Casos e Controles
Feminino
Perfilação da Expressão Gênica
Genômica
Seres Humanos
Meia-Idade
Transdução de Sinais
Biologia de Sistemas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170929
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pcbi.1005680


  10 / 10108 MEDLINE  
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[PMID]:28934507
[Au] Autor:Juzenas S; Venkatesh G; Hübenthal M; Hoeppner MP; Du ZG; Paulsen M; Rosenstiel P; Senger P; Hofmann-Apitius M; Keller A; Kupcinskas L; Franke A; Hemmrich-Stanisak G
[Ad] Endereço:Institute of Clinical Molecular Biology, Christian-Albrechts-University of Kiel, 24105 Kiel, Germany.
[Ti] Título:A comprehensive, cell specific microRNA catalogue of human peripheral blood.
[So] Source:Nucleic Acids Res;45(16):9290-9301, 2017 Sep 19.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:With this study, we provide a comprehensive reference dataset of detailed miRNA expression profiles from seven types of human peripheral blood cells (NK cells, B lymphocytes, cytotoxic T lymphocytes, T helper cells, monocytes, neutrophils and erythrocytes), serum, exosomes and whole blood. The peripheral blood cells from buffy coats were typed and sorted using FACS/MACS. The overall dataset was generated from 450 small RNA libraries using high-throughput sequencing. By employing a comprehensive bioinformatics and statistical analysis, we show that 3' trimming modifications as well as composition of 3' added non-templated nucleotides are distributed in a lineage-specific manner-the closer the hematopoietic progenitors are, the higher their similarities in sequence variation of the 3' end. Furthermore, we define the blood cell-specific miRNA and isomiR expression patterns and identify novel cell type specific miRNA candidates. The study provides the most comprehensive contribution to date towards a complete miRNA catalogue of human peripheral blood, which can be used as a reference for future studies. The dataset has been deposited in GEO and also can be explored interactively following this link: http://134.245.63.235/ikmb-tools/bloodmiRs.
[Mh] Termos MeSH primário: Células Sanguíneas/metabolismo
MicroRNAs/sangue
[Mh] Termos MeSH secundário: Linhagem da Célula
Eritrócitos/metabolismo
Exossomos/metabolismo
Seres Humanos
Linfócitos/metabolismo
MicroRNAs/química
Células Mieloides/metabolismo
Análise de Sequência de RNA
Transcriptoma
[Pt] Tipo de publicação:DATASET; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MicroRNAs)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170922
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx706



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