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  1 / 105 MEDLINE  
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[PMID]:26404440
[Au] Autor:Green R; Dwyre DM
[Ad] Endereço:Department of Pathology and Laboratory Medicine, University of California Davis Medical Center, Sacramento, CA. Electronic address: rgreen@ucdavis.edu.
[Ti] Título:Evaluation of Macrocytic Anemias.
[So] Source:Semin Hematol;52(4):279-86, 2015 Oct.
[Is] ISSN:1532-8686
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Macrocytic anemia, defined as a mean cell volume (MCV) ≥100 fL in adults, has a narrow differential diagnosis that requires evaluation of the peripheral blood smear as well as additional laboratory testing taken in conjunction with clinical information that includes patient history and physical examination findings. This review is an update on the approach to a patient with macrocytic anemia with attention paid to the differentiation of megaloblastic and non-megaloblastic macrocytic anemias. Critical to the determination of the diagnosis is the judicious use of laboratory testing and the evaluation of those findings in conjunction with the patient medical, surgical, and medication history.
[Mh] Termos MeSH primário: Anemia Macrocítica/diagnóstico
[Mh] Termos MeSH secundário: Diferenciação Celular
Diagnóstico Diferencial
Índices de Eritrócitos
Seres Humanos
Megaloblastos/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1602
[Cu] Atualização por classe:150925
[Lr] Data última revisão:
150925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150926
[St] Status:MEDLINE


  2 / 105 MEDLINE  
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Covas, Dimas Tadeu
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[PMID]:25472687
[Au] Autor:Brunetta DM; De Santis GC; Silva-Pinto AC; Oliveira de Oliveira LC; Covas DT
[Ad] Endereço:Center for Cell-Based Therapy, Medical School of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.
[Ti] Título:Hydroxyurea increases plasma concentrations of microparticles and reduces coagulation activation and fibrinolysis in patients with sickle cell anemia.
[So] Source:Acta Haematol;133(3):287-94, 2015.
[Is] ISSN:1421-9662
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Microparticles (MPs) are present in healthy subjects and their concentration increases in patients at high risk of thrombosis. We evaluated 10 patients with sickle cell anemia (SCA) treated with hydroxyurea (HU) and 13 SCA patients without this treatment. MP concentrations were determined by flow cytometry. Coagulation was evaluated using the thrombin-antithrombin complex (TAT) and D-dimers. Total MP concentrations were increased in the HU-treated group (265 × 10(6)/ml vs. 67.45 × 10(6)/ml; p = 0.0026), as well as MPs derived from RBC (67.83 × 10(6)/ml vs. 26.31 × 10(6)/ml; p = 0.05), monocytes (51.31 × 10(6)/ml vs. 9.03 × 10(6)/ml; p = 0.0084), monocytes with tissue factor (TF) expression (2.27 × 10(6)/ml vs. 0.27 × 10(6)/ml; p = 0.0058), endothelium (49.42 × 10(6)/ml vs. 7.23 × 10(6)/ml; p = 0.007) and endothelium with TF (1.42 × 10(6)/ml vs. 0.26 × 10(6)/ml; p = 0.0043). Furthermore, the concentrations of TAT (7.56 vs. 10.98 µg/l; p = 0.014) and D-dimers (0.65 vs. 1.29 µg/ml; p = 0.007) were reduced with HU. The MP elevation may suggest a direct cytotoxic effect of HU. Another explanation is a cell surface increase secondary to a megaloblastic process, resulting in increased vesicle release. In our opinion, the known benefits of HU on SCA patients, along with the reduction in coagulation activation, surpass its potential detrimental effect on MPs. Future studies should elucidate the role of MPs and demonstrate their significance in different contexts.
[Mh] Termos MeSH primário: Anemia Falciforme/sangue
Anemia Falciforme/tratamento farmacológico
Antidrepanocíticos/administração & dosagem
Micropartículas Derivadas de Células/metabolismo
Fibrinólise/efeitos dos fármacos
Hidroxiureia/administração & dosagem
[Mh] Termos MeSH secundário: Adulto
Anemia Falciforme/patologia
Animais
Antitrombinas/sangue
Endotélio Vascular/metabolismo
Endotélio Vascular/patologia
Feminino
Produtos de Degradação da Fibrina e do Fibrinogênio/metabolismo
Regulação da Expressão Gênica/efeitos dos fármacos
Seres Humanos
Masculino
Megaloblastos/metabolismo
Megaloblastos/patologia
Monócitos/metabolismo
Monócitos/patologia
Tromboplastina/biossíntese
[Pt] Tipo de publicação:CLINICAL TRIAL; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antisickling Agents); 0 (Antithrombins); 0 (Fibrin Fibrinogen Degradation Products); 0 (fibrin fragment D); 9035-58-9 (Thromboplastin); X6Q56QN5QC (Hydroxyurea)
[Em] Mês de entrada:1506
[Cu] Atualização por classe:150415
[Lr] Data última revisão:
150415
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141205
[St] Status:MEDLINE
[do] DOI:10.1159/000362148


  3 / 105 MEDLINE  
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[PMID]:23099925
[Au] Autor:Ohgiya D; Matsushita H; Kojima M; Ando K
[Ad] Endereço:Department of Hematology/Oncology, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa 259-1193, Japan. giya_pen_1213.0@triton.ocn.ne.jp
[Ti] Título:Huge multinucleated megaloblasts found in a patient with refractory anemia with excess blasts-1.
[So] Source:Int J Hematol;96(5):531-2, 2012 Nov.
[Is] ISSN:1865-3774
[Cp] País de publicação:Japan
[La] Idioma:eng
[Mh] Termos MeSH primário: Anemia Refratária com Excesso de Blastos/patologia
Núcleo Celular/patologia
Células Gigantes/patologia
Megaloblastos/patologia
[Mh] Termos MeSH secundário: Idoso
Seres Humanos
Masculino
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1311
[Cu] Atualização por classe:121126
[Lr] Data última revisão:
121126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121027
[St] Status:MEDLINE
[do] DOI:10.1007/s12185-012-1212-9


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[PMID]:22893049
[Au] Autor:Kächele V; Schneider-Kappus W; Bommer M
[Ad] Endereço:Hämato-Onkologische Praxis Ulm. volker.kaechele@gmx.de
[Ti] Título:[Pronounced pancytopenia with concomitant jaundice in a 66-year-old woman].
[Ti] Título:Ausgeprägte Trizytopenie mit Ikterus bei einer 66-jährigen Patientin..
[So] Source:Dtsch Med Wochenschr;137(34-35):1693-6, 2012 Aug.
[Is] ISSN:1439-4413
[Cp] País de publicação:Germany
[La] Idioma:ger
[Ab] Resumo:HISTORY AND ADMISSION FINDINGS: A previously healthy 66-year-old women presented with onset of general weakness, shortness of breath and significant weight loss. Due to appearance of jaundice, biliary obstruction had been ruled out by a CAT scan previous to the patients presentation in our practice. INVESTIGATIONS: The laboratory tests already arranged by the patients general practitioner showed a pronounced pancytopenia with megaloblastic anemia and hyperbilirubinemia. The bone marrow aspiration revealed a hypercellular bone marrow with megaloblastic erythropoiesis. The diagnosis of pernicious anemia was confirmed by the low cobalamin (vitamin B12) serum level and the presence of atrophic gastritis. TREATMENT: Pernicious anemia was treated with intramuscular injection of Cyanocobalamin (1000 µg) which resulted in an immediate reticulocytosis and a widely normalized blood cell count and bilirubin level four weeks after initiation of treatment. CONCLUSION: The differential diagnosis of megaloblastic anemia covers a wide spectrum of diseases with different etiology. This case report demonstrates an example of a pernicious anemia with atypical and foudroyant clinical course.
[Mh] Termos MeSH primário: Doenças Autoimunes/diagnóstico
Gastrite Atrófica/diagnóstico
Icterícia/etiologia
Pancitopenia/etiologia
[Mh] Termos MeSH secundário: Idoso
Anemia Macrocítica/tratamento farmacológico
Anemia Macrocítica/etiologia
Doenças Autoimunes/tratamento farmacológico
Biópsia
Medula Óssea/patologia
Diagnóstico Diferencial
Índices de Eritrócitos
Feminino
Gastrite Atrófica/tratamento farmacológico
Gastroscopia
Seres Humanos
Infusões Intravenosas
Icterícia/tratamento farmacológico
Megaloblastos/efeitos dos fármacos
Megaloblastos/patologia
Pancitopenia/tratamento farmacológico
Ultrassonografia
Vitamina B 12/administração & dosagem
[Pt] Tipo de publicação:CASE REPORTS; ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
P6YC3EG204 (Vitamin B 12)
[Em] Mês de entrada:1210
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120816
[St] Status:MEDLINE
[do] DOI:10.1055/s-0032-1305226


  5 / 105 MEDLINE  
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[PMID]:20533375
[Au] Autor:Renda MC; Giambona A; Fecarotta E; Leto F; Makrydimas G; Renda D; Damiani G; Jakil MC; Picciotto F; Piazza A; Valtieri M; Maggio A
[Ad] Endereço:Hematology II-Thalassemia, Ospedali Riuniti Villa Sofia-Cervello, Palermo, Italy.
[Ti] Título:Embryo-fetal erythroid megaloblasts in the human coelomic cavity.
[So] Source:J Cell Physiol;225(2):385-9, 2010 Nov.
[Is] ISSN:1097-4652
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The coelomic cavity is part of the extraembryonic mesoderm, surrounding amniotic cavity, embryo, and yolk sac in the early gestation. It is now believed to represent an important transfer interface and a reservoir of nutrients for the embryo. Coelocentesis by ultrasound-guided transvaginal puncture offers an easier access to the early human embryo, from 28 days post-fertilization. However, despite some studies about its biochemical composition being reported, our knowledge about the presence of cellular elements and their quality in this compartment are still limited. Here we studied human coelomic fluids sampled from 6.6 (48 days) to 10 weeks of gestation, demonstrating the presence of functional embryonic erythroid precursors, that is, megaloblasts in the coelomic cavity. The ease of access of the coelomic cavity could allow the development of novel strategies for diagnostic or therapeutic purposes by ultrasound imaging and ultrasound-guided puncture.
[Mh] Termos MeSH primário: Líquidos Corporais/citologia
Embrião de Mamíferos/citologia
Megaloblastos/fisiologia
[Mh] Termos MeSH secundário: Antígenos CD/metabolismo
Embrião de Mamíferos/fisiologia
Citometria de Fluxo
Regulação da Expressão Gênica no Desenvolvimento
Seres Humanos
Antígenos Comuns de Leucócito
Reação em Cadeia da Polimerase/métodos
Receptores da Transferrina/metabolismo
Saco Vitelino/fisiologia
Globinas épsilon/genética
Globinas épsilon/metabolismo
gama-Globinas/genética
gama-Globinas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antigens, CD); 0 (CD71 antigen); 0 (Receptors, Transferrin); 0 (epsilon-Globins); 0 (gamma-Globins); EC 3.1.3.48 (Leukocyte Common Antigens); EC 3.1.3.48 (PTPRC protein, human)
[Em] Mês de entrada:1010
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100610
[St] Status:MEDLINE
[do] DOI:10.1002/jcp.22269


  6 / 105 MEDLINE  
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[PMID]:18421190
[Au] Autor:Oka Y; Kameoka J; Hirabayashi Y; Takahashi R; Ishii T; Sasaki T; Harigae H
[Ad] Endereço:Department of Rheumatology and Hematology, Tohoku University Graduate School of Medicine, Sendai.
[Ti] Título:Reversible bone marrow dysplasia in patients with systemic lupus erythematosus.
[So] Source:Intern Med;47(8):737-42, 2008.
[Is] ISSN:1349-7235
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Several reports of bone marrow dysplasia in patients with systemic lupus erythematosus (SLE) have been published. However, the reports are restricted primarily to descriptions of the erythroid lineage; no follow-up studies have been reported, and the clinical significance of the dysplasias is unknown. Therefore, in the present study, the dysplasias noted in bone marrow aspirates obtained from SLE patients were characterized. PATIENTS AND METHODS: The smears of bone marrow aspirates obtained from 17 SLE patients who had bone marrow aspiration due to cytopenia (WBC < 1,500/microl, or Hb < 10.5 g/dl, or platelet count < 10 x 10(4)/microl) were examined retrospectively. Of the 17 patients, 4 had a repeat bone marrow aspiration during follow-up. Clinical and laboratory data were obtained from the medical records. RESULTS: Of the 17 SLE patients, 12 had dysplasias, including: erythroid cell multinuclearity (trinuclear or more) (5 patients), megaloblastoid changes (4), pseudo-Pelger abnormalities (6), annular nuclear myeloid cells (2), separated nuclear megakaryocytes (4), and micromegakaryocytes (5). In the 4 patients who had follow-up bone marrow aspiration, these dysplasias were correlated with disease activity; some abnormalities disappeared with remission of SLE. Diffuse proliferative glomerulonephritis (3 patients) and cerebral lupus/neuropsychiatric lupus (4 patients) were seen only in patients with dysplasia. CONCLUSION: This study found that bone marrow dysplasia can be observed in all lineage cells of SLE patients, and that the dysplasia is reversible during the course of the disease. The presence of dysplasias appears to be associated with disease severity.
[Mh] Termos MeSH primário: Doenças do Desenvolvimento Ósseo/etiologia
Doenças do Desenvolvimento Ósseo/patologia
Medula Óssea/patologia
Lúpus Eritematoso Sistêmico/complicações
Lúpus Eritematoso Sistêmico/patologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Biópsia por Agulha Fina
Criança
Feminino
Seres Humanos
Masculino
Megacariócitos/patologia
Megaloblastos/patologia
Meia-Idade
Células Mieloides/patologia
Remissão Espontânea
Estudos Retrospectivos
Índice de Gravidade de Doença
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:0806
[Cu] Atualização por classe:080418
[Lr] Data última revisão:
080418
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:080419
[St] Status:MEDLINE


  7 / 105 MEDLINE  
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[PMID]:18044723
[Au] Autor:Tsujioka T; Tochigi A; Kishimoto M; Kondo T; Tasaka T; Wada H; Sugihara T; Yoshida Y; Tohyama K
[Ad] Endereço:Department of Laboratory Medicine, Kawasaki Medical School, Okayama 701-0192, Japan.
[Ti] Título:DNA ploidy and cell cycle analyses in the bone marrow cells of patients with megaloblastic anemia using laser scanning cytometry.
[So] Source:Cytometry B Clin Cytom;74(2):104-9, 2008 Mar.
[Is] ISSN:1552-4957
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Megaloblastic anemias are characterized by several hematopoietic cells with dysplastic nuclear morphology. The analyses of DNA ploidy and cell cycle of these cells are important to understand the property of such diseases. METHODS: As laser scanning cytometry (LSC) is a useful tool to evaluate the morphology of the cells fixed on the slide glass together with the quantitative analysis of the fluorescence information of each cell by rapid scanning of the specimens, the authors examined the DNA ploidy and cell cycle of six cases with megaloblastic anemia using LSC. RESULTS: Giant neutrophilic series such as giant metamyelocytes and giant band cells were found to have extraordinarily higher DNA ploidy, while hypersegmented neutrophils represented the normal diploid pattern like normal neutrophils. As to megaloblasts, cell cycle analysis showed that the proportion of the cells in S phase was increased as compared with the case of normal erythroblasts. CONCLUSIONS: The present study clearly demonstrates the abnormal aspects of the hematopoietic cells with megaloblastic anemia from the viewpoint of the DNA ploidy and cell cycle analyzed by the use of LSC.
[Mh] Termos MeSH primário: Anemia Megaloblástica/diagnóstico
Anemia Megaloblástica/genética
Células da Medula Óssea/patologia
Ciclo Celular/genética
Aberrações Cromossômicas
Citometria de Varredura a Laser/métodos
Ploidias
[Mh] Termos MeSH secundário: Anemia Megaloblástica/sangue
Núcleo Celular/patologia
DNA/genética
Células Gigantes/patologia
Células Precursoras de Granulócitos/patologia
Células HL-60
Células-Tronco Hematopoéticas/patologia
Seres Humanos
Megaloblastos/patologia
Neutrófilos/patologia
Fase S/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9007-49-2 (DNA)
[Em] Mês de entrada:0805
[Cu] Atualização por classe:091211
[Lr] Data última revisão:
091211
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:071130
[St] Status:MEDLINE


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[PMID]:17301818
[Au] Autor:Verburgh E; Achten R; Louw VJ; Brusselmans C; Delforge M; Boogaerts M; Hagemeijer A; Vandenberghe P; Verhoef G
[Ad] Endereço:Department of Hematology, University Hospitals, University of Leuven, Leuven, Belgium. estelle.verburgh@student.kuleuven.be
[Ti] Título:A new disease categorization of low-grade myelodysplastic syndromes based on the expression of cytopenia and dysplasia in one versus more than one lineage improves on the WHO classification.
[So] Source:Leukemia;21(4):668-77, 2007 Apr.
[Is] ISSN:0887-6924
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Multilineage dysplasia was advanced by the World Health Organization to increase prognostic accuracy in myelodysplastic syndromes (MDS) classification. We performed a structured cytomorphological examination of bone marrow (BM) in 221 low-grade MDS patients, this in conjunction with strict guidelines for cytopenias. A dysplasia scoring system was developed utilizing dysplastic changes, which were associated with worse outcome on univariate and multivariate analysis corrected for the International Prognostic Scoring System (IPSS). Dysplasia >or=10% in one BM lineage and one cytopenia constituted the low-risk category UCUD or Unilineage Cytopenia and Unilineage Dysplasia. The high-risk category comprised patients with cytopenia in >or=2 lineages and dysplasia in >or=2 BM lineages, namely MCMD or Multilineage Cytopenia and Multilineage Dysplasia. Intermediate-risk patients had one cytopenia and multilineage dysplasia, or cytopenia in >or=2 lineages and unilineage BM dysplasia, designated UCMD/MCUD or Unilineage Cytopenia and Multilineage Dysplasia/Multilineage Cytopenia and Unilineage Dysplasia. This system utilizing cytopenia-dysplasia scoring at diagnosis enabled comprehensive categorization of low-grade MDS cases that predicted for overall as well as leukemia-free survival. Cytopenia-dysplasia categorization added additional prognostic values to the lower risk IPSS categories. This suggests that a standardized dysplasia scoring system, used in conjunction with cytopenia, could improve diagnostic and prognostic sub-categorization of MDS patients.
[Mh] Termos MeSH primário: Megaloblastos/patologia
Síndromes Mielodisplásicas/classificação
[Mh] Termos MeSH secundário: Análise de Variância
Medula Óssea/patologia
Células da Medula Óssea/patologia
Cariotipagem
Análise Multivariada
Síndromes Mielodisplásicas/genética
Síndromes Mielodisplásicas/mortalidade
Síndromes Mielodisplásicas/patologia
Análise de Sobrevida
Organização Mundial da Saúde
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:0709
[Cu] Atualização por classe:130304
[Lr] Data última revisão:
130304
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070216
[St] Status:MEDLINE


  9 / 105 MEDLINE  
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PubMed Central Texto completo
[PMID]:16988104
[Au] Autor:Aslinia F; Mazza JJ; Yale SH
[Ad] Endereço:Department of Internal Medicine, Marshfield Clinic, 1000 North Oak Avenue, Marshfield, WI 54449, USA.
[Ti] Título:Megaloblastic anemia and other causes of macrocytosis.
[So] Source:Clin Med Res;4(3):236-41, 2006 Sep.
[Is] ISSN:1539-4182
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Anemia Megaloblástica/sangue
[Mh] Termos MeSH secundário: Anemia Macrocítica/sangue
Anemia Macrocítica/etiologia
Anemia Megaloblástica/etiologia
Deficiência de Ácido Fólico/sangue
Deficiência de Ácido Fólico/complicações
Deficiência de Ácido Fólico/tratamento farmacológico
Seres Humanos
Megaloblastos/patologia
Deficiência de Vitamina B 12/sangue
Deficiência de Vitamina B 12/complicações
Deficiência de Vitamina B 12/tratamento farmacológico
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:0611
[Cu] Atualização por classe:140908
[Lr] Data última revisão:
140908
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:060922
[St] Status:MEDLINE


  10 / 105 MEDLINE  
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[PMID]:16740048
[Au] Autor:Svensson AM; Pang Y; Moore NJ; Tindle BH
[Ad] Endereço:Department of Pathology and Laboratory Medicine, University of Vermont, Fletcher Allen Health Care, Burlington, VT 05401, USA. Annika.Svensson@uvm.edu
[Ti] Título:Cystic tumor of the cerebellum with megaloblastic erythropoiesis. Hemangioblastoma with megaloblastic hematopoiesis.
[So] Source:Arch Pathol Lab Med;130(6):886-9, 2006 Jun.
[Is] ISSN:1543-2165
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Neoplasias Cerebelares/patologia
Eritropoese
Hemangioblastoma/patologia
Megaloblastos/citologia
[Mh] Termos MeSH secundário: Biomarcadores Tumorais/análise
Carcinoma de Células Renais/diagnóstico
Carcinoma de Células Renais/secundário
Neoplasias Cerebelares/química
Neoplasias Cerebelares/cirurgia
Cerebelo/patologia
Diagnóstico Diferencial
Hemangioblastoma/química
Hemangioblastoma/cirurgia
Hematopoese Extramedular/fisiologia
Seres Humanos
Neoplasias Renais/diagnóstico
Neoplasias Renais/secundário
Imagem por Ressonância Magnética
Masculino
Megaloblastos/química
Megaloblastos/fisiologia
Meia-Idade
Fosfopiruvato Hidratase/análise
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor); EC 4.2.1.11 (Phosphopyruvate Hydratase)
[Em] Mês de entrada:0606
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:060603
[St] Status:MEDLINE



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