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Pesquisa : A11.118.290.330.802 [Categoria DeCS]
Referências encontradas : 168 [refinar]
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[PMID]:28150311
[Au] Autor:Roussel C; Dussiot M; Marin M; Morel A; Ndour PA; Duez J; Le Van Kim C; Hermine O; Colin Y; Buffet PA; Amireault P
[Ad] Endereço:Université Sorbonne Paris Cité, Université Paris Descartes, Inserm, INTS, Unité Biologie Intégrée du Globule Rouge.
[Ti] Título:Spherocytic shift of red blood cells during storage provides a quantitative whole cell-based marker of the storage lesion.
[So] Source:Transfusion;57(4):1007-1018, 2017 Apr.
[Is] ISSN:1537-2995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Storage lesion may explain the rapid clearance of up to 25% of transfused red blood cells (RBCs) in recipients. Several alterations affect stored RBC but a quantitative, whole cell-based predictor of transfusion yield is lacking. Because RBCs with reduced surface area are retained by the spleen, we quantified changes in RBC dimensions during storage. STUDY DESIGN AND METHODS: Using imaging flow cytometry we observed the dimension and morphology of RBCs upon storage, along with that of conventional biochemical and mechanical markers of storage lesion. We then validated these findings using differential interference contrast (DIC) microscopy and quantified the accumulation of microparticles (MPs). RESULTS: Mean projected surface area of the whole RBC population decreased from 72.4 to 68.4 µm , a change resulting from the appearance of a well-demarcated subpopulation of RBCs with reduced mean projected surface (58 µm , 15.2%-19.9% reduction). These "small RBCs" accounted for 4.9 and 23.6% of all RBCs on Days 3 and 42 of storage, respectively. DIC microscopy confirmed that small RBCs had shifted upon storage from discocytes to echinocytes III, spheroechinocytes, and spherocytes. Glycophorin A-positive MPs and small RBCs appeared after similar kinetics. CONCLUSION: The reduction in surface area of small RBCs is expected to induce their retention by the spleen. We propose that small RBCs generated by MP-induced membrane loss are preferentially cleared from the circulation shortly after transfusion of long-stored blood. Their operator-independent quantification using imaging flow cytometry may provide a marker of storage lesion potentially predictive of transfusion yield.
[Mh] Termos MeSH primário: Preservação de Sangue
Micropartículas Derivadas de Células
Citometria de Fluxo/métodos
Esferócitos/citologia
[Mh] Termos MeSH secundário: Biomarcadores/sangue
Feminino
Seres Humanos
Cinética
Masculino
Esferócitos/metabolismo
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170621
[Lr] Data última revisão:
170621
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170203
[St] Status:MEDLINE
[do] DOI:10.1111/trf.14015


  2 / 168 MEDLINE  
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[PMID]:27823366
[Au] Autor:Cluzel C; Javard R; Grimes C
[Ti] Título:Pathology in Practice.
[So] Source:J Am Vet Med Assoc;249(10):1153-1155, 2016 Nov 15.
[Is] ISSN:1943-569X
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Anemia Hemolítica Autoimune/veterinária
Doenças do Cão/patologia
[Mh] Termos MeSH secundário: Anemia Hemolítica Autoimune/diagnóstico
Anemia Hemolítica Autoimune/patologia
Animais
Transfusão de Sangue/veterinária
Doenças do Cão/diagnóstico
Cães
Neutrófilos
Esferócitos
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161109
[St] Status:MEDLINE


  3 / 168 MEDLINE  
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[PMID]:26926169
[Au] Autor:Hashemi Z; Rahnama M; Jafari S
[Ti] Título:Lattice Boltzmann Simulation of Healthy and Defective Red Blood Cell Settling in Blood Plasma.
[So] Source:J Biomech Eng;138(5):051002, 2016 May.
[Is] ISSN:1528-8951
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this paper, an attempt has been made to study sedimentation of a red blood cell (RBC) in a plasma-filled tube numerically. Such behaviors are studied for a healthy and a defective cell which might be created due to human diseases, such as diabetes, sickle-cell anemia, and hereditary spherocytosis. Flow-induced deformation of RBC is obtained using finite-element method (FEM), while flow and fluid-membrane interaction are handled using lattice Boltzmann (LB) and immersed boundary methods (IBMs), respectively. The effects of RBC properties as well as its geometry and orientation on its sedimentation rate are investigated and discussed. The results show that decreasing frontal area of an RBC and/or increasing tube diameter results in a faster settling. Comparison of healthy and diabetic cells reveals that less cell deformability leads to slower settling. The simulation results show that the sicklelike and spherelike RBCs have lower settling velocity as compared with a biconcave discoid cell.
[Mh] Termos MeSH primário: Eritrócitos/citologia
Eritrócitos/patologia
Análise de Elementos Finitos
Fenômenos Mecânicos
Modelos Biológicos
Plasma/citologia
[Mh] Termos MeSH secundário: Anemia Falciforme/sangue
Fenômenos Biomecânicos
Sedimentação Sanguínea
Diabetes Mellitus/sangue
Deformação Eritrocítica
Membrana Eritrocítica/metabolismo
Eritrócitos/fisiologia
Seres Humanos
Esferócitos/citologia
Esferócitos/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160302
[St] Status:MEDLINE
[do] DOI:10.1115/1.4032851


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[PMID]:26336967
[Au] Autor:Andres O; Eber S; Speer CP
[Ad] Endereço:University Children's Hospital, University of Würzburg, Josef-Schneider-Straße 2, 97080, Würzburg, Germany. andres_o@ukw.de.
[Ti] Título:Early postnatal diagnosis of hereditary spherocytosis by combining light microscopy, acidified glycerol lysis test and eosin-5'-maleimide binding assay.
[So] Source:Ann Hematol;94(12):1959-64, 2015 Dec.
[Is] ISSN:1432-0584
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Exact diagnosis of hereditary spherocytosis (HS) is widely considered unreliable around birth. However, early postnatal diagnosis at the beginning of congenital hemolysis may be essential for managing neonatal anemia and hemolytic icterus, identifying those at high risk for severe hyperbilirubinemia, irreversible kernicterus, or sudden need for red cell transfusion. We analyzed 37 blood samples from neonates or infants up to six weeks of life that had been collected in-house or shipped to our laboratory due to suspected red cell membrane disorder. By combining assessment of red cell morphology, acidified glycerol lysis test (AGLT), and eosin-5'-maleimide (EMA) binding assay, we were able to clearly exclude HS in 22 and confirm HS in 10 patients, of which one had undergone red cell transfusion prior to blood sampling. Assessment of red cell morphology and normal test results allowed diagnosis of infantile pyknocytosis or Heinz body anemia in three neonates. Re-evaluation of five patients with inconsistent results of AGLT and EMA binding led to confirmation of HS in two cases. Automated analysis of hematologic parameters revealed elevated proportion of hyperdense cells to be a highly significant indicator for HS in neonatal infants. We showed that assessment of red cell morphology in combination with AGLT and EMA binding assay is a reliable basis for confirming or rejecting suspected diagnosis of HS even in neonates. Our data underline the necessity for blood sampling and laboratory exploration in suspected red cell membrane or enzyme defects at the earliest occasion.
[Mh] Termos MeSH primário: Amarelo de Eosina-(YS)/análogos & derivados
Membrana Eritrocítica
Glicerol/química
Esferócitos
Esferocitose Hereditária/diagnóstico
[Mh] Termos MeSH secundário: Amarelo de Eosina-(YS)/química
Membrana Eritrocítica/química
Membrana Eritrocítica/patologia
Feminino
Seres Humanos
Lactente
Recém-Nascido
Masculino
Esferócitos/química
Esferócitos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
76296-42-9 (eosin maleimide); PDC6A3C0OX (Glycerol); TDQ283MPCW (Eosine Yellowish-(YS))
[Em] Mês de entrada:1601
[Cu] Atualização por classe:151014
[Lr] Data última revisão:
151014
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150905
[St] Status:MEDLINE
[do] DOI:10.1007/s00277-015-2491-z


  5 / 168 MEDLINE  
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[PMID]:26275251
[Au] Autor:Nair SC; Arora N; Jain S; Inbakumar D; Mammen J; Sitaram U
[Ti] Título:Mean reticulocyte volume enhances the utility of red cell mean sphered cell volume in differentiating peripheral blood spherocytes of hereditary spherocytosis from other causes.
[So] Source:Indian J Pathol Microbiol;58(3):307-9, 2015 Jul-Sep.
[Is] ISSN:0974-5130
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:CONTEXT: Mean sphered cell volume (MSCV) and mean reticulocyte volume (MRV) are additional reticulocyte parameters generated while processing the blood samples on Beckman coulter LH 755 in the reticulocyte mode using the volume, conductivity and scatter technology. It has been observed that the difference between mean corpuscular volume (MCV) and MSCV is higher in the cases of hereditary spherocytosis (HS) and this difference is increasingly being utilized as a screening tool for spherocytes. In addition now there have been new observations that reticulocyte volume in cases of HS is less as compared to normal reticulocyte. AIMS: Our aim was to test the usefulness of reticulocyte parameters like MSCV and MRV in distinguishing cases of HS and autoimmune hemolytic anemia (AIHA). MATERIALS AND METHODS: This is a retrospective and partly prospective study where peripheral blood ethylenediaminetetraacetic acid samples from cases of HS (n = 57) and AIHA (n = 29) were processed on LH 755 in both the differential and the reticulocyte mode. The data generated were analyzed and compared with data from normal healthy donors (n = 46). RESULTS: Using an algorithm of MCV - MSCV >10 and MRV - MSCV <25, a sensitivity of 84.2% and specificity of 94.7% was observed in cases of HS. CONCLUSIONS: With the reticulocyte analysis, we may now have a simple and cheap additional tool for screening of HS.
[Mh] Termos MeSH primário: Anemia Hemolítica Autoimune/diagnóstico
Anemia Hemolítica Autoimune/patologia
Reticulócitos/fisiologia
Esferócitos/patologia
Esferocitose Hereditária/diagnóstico
Esferocitose Hereditária/patologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Tamanho Celular
Criança
Pré-Escolar
Feminino
Seres Humanos
Lactente
Recém-Nascido
Masculino
Meia-Idade
Estudos Prospectivos
Estudos Retrospectivos
Sensibilidade e Especificidade
Adulto Jovem
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Em] Mês de entrada:1604
[Cu] Atualização por classe:150815
[Lr] Data última revisão:
150815
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150815
[St] Status:MEDLINE
[do] DOI:10.4103/0377-4929.162836


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[PMID]:25357094
[Au] Autor:Christensen RD; Agarwal AM; Nussenzveig RH; Heikal N; Liew MA; Yaish HM
[Ad] Endereço:1] The Department of Women and Newborns, Intermountain Healthcare, Salt Lake City, UT, USA [2] Division of Neonatology, University of Utah School of Medicine, Salt Lake City, UT, USA [3] Division of Hematology/Oncology, Department of Pediatrics, University of Utah School of Medicine, Salt Lake City,
[Ti] Título:Evaluating eosin-5-maleimide binding as a diagnostic test for hereditary spherocytosis in newborn infants.
[So] Source:J Perinatol;35(5):357-61, 2015 May.
[Is] ISSN:1476-5543
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Neonates with undiagnosed hereditary spherocytosis (HS) are at risk for developing hazardous hyperbilirubinemia and anemia. Making an early diagnosis of HS in a neonate can prompt anticipatory guidance to prevent these adverse outcomes. A recent comparison study showed that a relatively new diagnostic test for HS, eosin-5-maleimide (EMA)-flow cytometry, performs better than other available tests in confirming HS. However, reports have not specifically examined the performance of this test among neonates. STUDY DESIGN: We compared EMA-flow cytometry from blood samples of healthy control neonates vs samples from neonates suspected of having HS on the basis of severe Coombs-negative jaundice and spherocytes on blood film. The diagnosis of HS was later either confirmed or excluded based on clinical findings and next generation sequencing (NGS) after which we correlated the EMA-flow results with the diagnosis. RESULT: EMA-flow was performed on the blood of 31 neonates; 20 healthy term newborns and 11 who were suspected of having HS. Eight of the 11 were later confirmed positive for HS and one was confirmed positive for hereditary elliptocytosis (HE). All nine had persistently abnormal erythroid morphology, reticulocytosis and anemia, and eight of the nine had relevant mutations discovered using NGS. The other was confirmed positive for HS on the basis that a parent had HS, and the neonate's spherocytosis, reticulocytosis and anemia persisted. The 20 healthy controls and the 2 in whom HS was initially suspected but later excluded all had EMA-flow results in the range reported in healthy children and adults. In contrast, all nine in whom HS or HE was confirmed had abnormal EMA-flow results consistent with previous reports in older children and adults with HS. CONCLUSION: Although our sample size is small, our findings are consistent with the literature in older children and adults suggesting that EMA-flow cytometric testing performs well in supporting the diagnosis of HS/HE during the early neonatal period.
[Mh] Termos MeSH primário: Amarelo de Eosina-(YS)/análogos & derivados
Triagem Neonatal
Esferocitose Hereditária/diagnóstico
Esferocitose Hereditária/genética
[Mh] Termos MeSH secundário: Estudos de Casos e Controles
Amarelo de Eosina-(YS)/análise
Citometria de Fluxo
Sequenciamento de Nucleotídeos em Larga Escala
Seres Humanos
Recém-Nascido
Esferócitos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
76296-42-9 (eosin maleimide); TDQ283MPCW (Eosine Yellowish-(YS))
[Em] Mês de entrada:1601
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141031
[St] Status:MEDLINE
[do] DOI:10.1038/jp.2014.202


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[PMID]:25121167
[Au] Autor:Mantripragada K; Quesenberry PJ
[Ti] Título:Doublet spherocytes.
[So] Source:Blood;124(1):12, 2014 Jul 03.
[Is] ISSN:1528-0020
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Anemia Hemolítica Autoimune/patologia
Esferócitos/patologia
Trombocitopenia/patologia
[Mh] Termos MeSH secundário: Anemia Hemolítica Autoimune/fisiopatologia
Feminino
Seres Humanos
Meia-Idade
Trombocitopenia/fisiopatologia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Evans Syndrome)
[Em] Mês de entrada:1408
[Cu] Atualização por classe:150527
[Lr] Data última revisão:
150527
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:140815
[St] Status:MEDLINE


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[PMID]:24016137
[Au] Autor:McIlwaine K; Leach MT
[Ad] Endereço:Department of Haematology, Gartnavel Hospital, Glasgow, UK.
[Ti] Título:Clostridium perfringens septicaemia.
[So] Source:Br J Haematol;163(5):549, 2013 Dec.
[Is] ISSN:1365-2141
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Bacteriemia/sangue
Infecções por Clostridium/sangue
Clostridium perfringens/isolamento & purificação
[Mh] Termos MeSH secundário: Dor Abdominal/etiologia
Idoso
Bacteriemia/etiologia
Bacteriemia/microbiologia
Carcinoma/complicações
Carcinoma/microbiologia
Infecções por Clostridium/etiologia
Neoplasias do Colo/complicações
Neoplasias do Colo/microbiologia
Evolução Fatal
Seres Humanos
Masculino
Esferócitos/ultraestrutura
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1401
[Cu] Atualização por classe:131113
[Lr] Data última revisão:
131113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130911
[St] Status:MEDLINE
[do] DOI:10.1111/bjh.12551


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[PMID]:23624969
[Au] Autor:Yaish HM; Christensen RD; Agarwal A
[Ad] Endereço:Division of Hematology/Oncology, Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, UT, USA.
[Ti] Título:A neonate with Coombs-negative hemolytic jaundice with spherocytes but normal erythrocyte indices: a rare case of autosomal-recessive hereditary spherocytosis due to alpha-spectrin deficiency.
[So] Source:J Perinatol;33(5):404-6, 2013 May.
[Is] ISSN:1476-5543
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The diagnosis of hereditary spherocytosis (HS) in a newborn infant is generally made on the basis of a positive family history, spherocytes on blood film and Coombs-negative hemolytic jaundice of variable severity with an elevated mean corpuscular hemoglobin concentration (MCHC) and a low mean corpuscular volume (MCV). In general, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) quantification of erythrocyte membrane proteins is not needed to make the clinical diagnosis of HS. However, we observed that a neonate with no family history of HS, but with abundant spherocytosis on repeated blood films, Coombs-negative hemolytic jaundice and normal MCHC and MCV measurements, where SDS-PAGE revealed alpha-spectrin deficiency, a rare autosomal-recessive variety of HS that generally has a severe clinical phenotype.
[Mh] Termos MeSH primário: Icterícia/sangue
Espectrina/deficiência
Esferocitose Hereditária/etiologia
[Mh] Termos MeSH secundário: Teste de Coombs
Índices de Eritrócitos
Seres Humanos
Recém-Nascido
Masculino
Esferócitos
Esferocitose Hereditária/sangue
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
12634-43-4 (Spectrin)
[Em] Mês de entrada:1310
[Cu] Atualização por classe:130429
[Lr] Data última revisão:
130429
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130430
[St] Status:MEDLINE
[do] DOI:10.1038/jp.2012.67


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[PMID]:22954295
[Au] Autor:Lane LV
[Ad] Endereço:Oklahoma State University, OK, USA.
[Ti] Título:Hemato--going--going--gone!
[So] Source:Vet Clin Pathol;41(3):311, 2012 Sep.
[Is] ISSN:1939-165X
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Anemia/veterinária
Doenças do Cão/sangue
Eritroblastos/citologia
Esferócitos/citologia
[Mh] Termos MeSH secundário: Anemia/sangue
Animais
Núcleo Celular/metabolismo
Cães
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1408
[Cu] Atualização por classe:161020
[Lr] Data última revisão:
161020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120908
[St] Status:MEDLINE
[do] DOI:10.1111/j.1939-165X.2012.00460.x



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