Base de dados : MEDLINE
Pesquisa : A11.118.480 [Categoria DeCS]
Referências encontradas : 3486 [refinar]
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  1 / 3486 MEDLINE  
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[PMID]:29181623
[Au] Autor:Qiu L; Chen MM; Wang RY; Wan XY; Li C; Zhang QL; Dong X; Yang B; Xiang JH; Huang J
[Ad] Endereço:Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture, Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Yellow
[Ti] Título:Complete genome sequence of shrimp hemocyte iridescent virus (SHIV) isolated from white leg shrimp, Litopenaeus vannamei.
[So] Source:Arch Virol;163(3):781-785, 2018 Mar.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Infection with shrimp hemocyte iridescent virus (SHIV), a new virus of the family Iridoviridae isolated in China, results in a high mortality rate in white leg shrimp (Litopenaeus vannamei). The complete genome sequence of SHIV was determined and analyzed in this study. The genomic DNA was 165,809 bp long with 34.6% G+C content and 170 open reading frames (ORFs). Dotplot analysis showed that the longest repetitive region was 320 bp in length, including 11 repetitions of an 18-bp sequence and 3.1 repetitions of a 39-bp sequence. Two phylogenetic trees were constructed based on 27 or 16 concatenated sequences of proteins encoded by genes that are conserved between SHIV homologous and other iridescent viruses. The results of this study, suggest that SHIV should be considered a member of the proposed new genus "Xiairidovirus".
[Mh] Termos MeSH primário: DNA Viral/genética
Genoma Viral
Iridovirus/genética
Penaeidae/virologia
Filogenia
[Mh] Termos MeSH secundário: Animais
Composição de Bases
Sequência de Bases
Hemócitos/virologia
Iridovirus/classificação
Iridovirus/isolamento & purificação
Fases de Leitura Aberta
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3642-4


  2 / 3486 MEDLINE  
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[PMID]:29265467
[Au] Autor:Guan J; Zhang J; Yuan S; Yang B; Clark KD; Ling E; Huang W
[Ad] Endereço:Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
[Ti] Título:Analysis of the functions of the signal peptidase complex in the midgut of Tribolium castaneum.
[So] Source:Arch Insect Biochem Physiol;97(3), 2018 Mar.
[Is] ISSN:1520-6327
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Signal peptidase complexes (SPCs) are conserved from bacteria to human beings, and are typically composed of four to five subunits. There are four genes encoding SPC proteins in the red flour beetle, Tribolium castaneum. To understand their importance to insect development, double-stranded RNA for each SPC gene was injected into red flour beetles at the early larval and adult stages. Knockdown of all four signal peptidase genes was lethal to larvae. Moreover, larvae had difficulty with old cuticle ecdysis. Knockdown of TcSPC12 alone did not affect pupal or adult development. When TcSPC12, TcSPC18, and TcSPC25 were knocked down in larvae, the melanization of hemocytes and midguts was observed. When knocked down in larvae and adults, TcSPC18 induced severe cell apoptosis in midguts, and the adult midgut lost the ability to maintain crypts after knockdown of TcSPC18, indicating its importance to midgut cell proliferation and differentiation. Knockdown of TcSPC22 or TcSPC25 also resulted in many apoptotic cells in the midguts. However, TcSPC12 appeared to be unimportant for midgut development. We conclude that TcSPC18 is essential for maintaining the adult midgut crypts.
[Mh] Termos MeSH primário: Proteínas de Membrana/metabolismo
Serina Endopeptidases/metabolismo
Tribolium/enzimologia
[Mh] Termos MeSH secundário: Animais
Feminino
Trato Gastrointestinal/enzimologia
Hemócitos/metabolismo
Proteínas de Insetos/metabolismo
Melaninas/metabolismo
Interferência de RNA
Tribolium/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insect Proteins); 0 (Melanins); 0 (Membrane Proteins); EC 3.4.21.- (Serine Endopeptidases); EC 3.4.21.89 (type I signal peptidase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE
[do] DOI:10.1002/arch.21441


  3 / 3486 MEDLINE  
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[PMID]:28745455
[Au] Autor:Hu QQ; Wei XH; Li YP; Wang JL; Liu XS
[Ad] Endereço:Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, China.
[Ti] Título:Identification and characterization of a gene involved in the encapsulation response of Helicoverpa armigera haemocytes.
[So] Source:Insect Mol Biol;26(6):752-762, 2017 12.
[Is] ISSN:1365-2583
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Encapsulation is a kind of cellular immune response of insect haemocytes, which results in the formation of capsules around invading parasites. However, the molecular mechanism of this response is largely unknown. In this study, we identified a potential immune-related gene in the cotton bollworm, Helicoverpa armigera, called defence protein 1 (Ha-DFP1). A tissue distribution analysis revealed that Ha-DFP1 protein was expressed in haemocytes and secreted into the haemolymph of Helic. armigera larvae. The Ha-DFP1 mRNA transcript level in haemocytes and the concentration of the Ha-DFP1 protein in haemolymph both increased after injecting chromatography beads. Purified recombinant Ha-DFP1 bound to the surface of haemocytes and promoted haemocyte encapsulation on chromatography beads in vitro. The spreading ability of haemocytes was inhibited when Ha-DFP1 expression in Helic. armigera larval haemocytes decreased in response to the injection of double-stranded RNA specific to Ha-DFP1, and the encapsulation ability of haemocytes was impaired. Based on these results, we speculate that Ha-DFP1 plays an important role in the Helic. armigera encapsulation response, possibly by binding to the haemocyte surface and mediating spreading behaviour.
[Mh] Termos MeSH primário: Hemócitos/fisiologia
Proteínas de Insetos/genética
Mariposas/genética
Mariposas/imunologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sequência de Bases
Genes de Insetos
Proteínas de Insetos/isolamento & purificação
Proteínas de Insetos/metabolismo
Mariposas/metabolismo
Interferência de RNA
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Insect Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:180111
[Lr] Data última revisão:
180111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.1111/imb.12336


  4 / 3486 MEDLINE  
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[PMID]:29028801
[Au] Autor:Del Signore SJ; Biber SA; Lehmann KS; Heimler SR; Rosenfeld BH; Eskin TL; Sweeney ST; Rodal AA
[Ad] Endereço:Rosenstiel Basic Medical Sciences Research Center, Department of Biology, Brandeis University, Waltham, Massachusetts, United States of America.
[Ti] Título:dOCRL maintains immune cell quiescence by regulating endosomal traffic.
[So] Source:PLoS Genet;13(10):e1007052, 2017 Oct.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Lowe Syndrome is a developmental disorder characterized by eye, kidney, and neurological pathologies, and is caused by mutations in the phosphatidylinositol-5-phosphatase OCRL. OCRL plays diverse roles in endocytic and endolysosomal trafficking, cytokinesis, and ciliogenesis, but it is unclear which of these cellular functions underlie specific patient symptoms. Here, we show that mutation of Drosophila OCRL causes cell-autonomous activation of hemocytes, which are macrophage-like cells of the innate immune system. Among many cell biological defects that we identified in docrl mutant hemocytes, we pinpointed the cause of innate immune cell activation to reduced Rab11-dependent recycling traffic and concomitantly increased Rab7-dependent late endosome traffic. Loss of docrl amplifies multiple immune-relevant signals, including Toll, Jun kinase, and STAT, and leads to Rab11-sensitive mis-sorting and excessive secretion of the Toll ligand Spåtzle. Thus, docrl regulation of endosomal traffic maintains hemocytes in a poised, but quiescent state, suggesting mechanisms by which endosomal misregulation of signaling may contribute to symptoms of Lowe syndrome.
[Mh] Termos MeSH primário: Citocinese/genética
Imunidade Inata/genética
Síndrome Oculocerebrorrenal/genética
Monoéster Fosfórico Hidrolases/genética
[Mh] Termos MeSH secundário: Animais
Drosophila
Endossomos/genética
Endossomos/patologia
Hemócitos/metabolismo
Hemócitos/patologia
Seres Humanos
Mutação
Síndrome Oculocerebrorrenal/patologia
Ligação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.1.3.2 (Phosphoric Monoester Hydrolases); EC 3.1.3.36 (OCRL protein, human)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171111
[Lr] Data última revisão:
171111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171014
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1007052


  5 / 3486 MEDLINE  
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[PMID]:28940716
[Au] Autor:Dong SM; Cui JH; Zhang W; Zhang XW; Kou TC; Cai QC; Xu S; You S; Yu DS; Ding L; Lai JH; Li M; Luo KJ
[Ad] Endereço:Key Laboratory for Animal Genetic Diversity and Evolution of High Education in Yunnan Province, School of Life Sciences, Yunnan University, Kunming, P.R. China.
[Ti] Título:Inhibition of translation initiation factor eIF4A is required for apoptosis mediated by Microplitis bicoloratus bracovirus.
[So] Source:Arch Insect Biochem Physiol;96(3), 2017 Nov.
[Is] ISSN:1520-6327
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Apoptotic hemocytes induced by Microplitis bicoloratus parasitism have been reported, and M. bicoloratus bracovirus (MbBV) is known to be the apoptosis inducer. However, the mechanism how MbBV regulates apoptosis remains unclear. eIF4A, one of translation initiation factors, was found from a Spodoptera litura transcriptome, the expression of which in the parasitized hemocytes of S. litura was inhibited in RT-qPCR analysis. The western blot also illustrated eIF4A at 6-day post-parasitization was inhibited in hemocytes. For testing interaction of MbBV-eIF4A-apoptosis, a cDNA clone encoding 1,266 bp of eIF4A was obtained from S. litura hemocytes and sequenced. Then, a 48 kDa V5-fusion protein of the eIF4A was detected by using the anti-V5 antibody at 72-h post-transfection in the High Five cells, which is located in the cell cytoplasm. In vitro, overexpression of eIF4A rescued the apoptotic High Five cells induced by MbBV. Conversely, in vivo, loss of eIF4A proteins by dsRNA feeding increased apoptosis of hemocytes. Furthermore, RNAi and parasitism significantly increased apoptosis of hemocytes in S. litura. These findings suggested that MbBV inhibited the expression of eIF4A, which was required for apoptosis mediated by MbBV. This study will contribute to biological pest control and enhance our understanding of molecular mechanisms underlying polydnavirus-parasitoid-host interaction.
[Mh] Termos MeSH primário: Apoptose/fisiologia
Fatores de Iniciação em Eucariotos/metabolismo
Hemócitos/metabolismo
Vírus dos Insetos/fisiologia
Mariposas/virologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Fatores de Iniciação em Eucariotos/genética
Regulação da Expressão Gênica
Interações Hospedeiro-Patógeno
Proteínas de Insetos/genética
Proteínas de Insetos/metabolismo
Mariposas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Eukaryotic Initiation Factors); 0 (Insect Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170924
[St] Status:MEDLINE
[do] DOI:10.1002/arch.21423


  6 / 3486 MEDLINE  
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[PMID]:28898595
[Au] Autor:Blanchoud S; Zondag L; Lamare MD; Wilson MJ
[Ti] Título:Hematological Analysis of the Ascidian Botrylloides leachii (Savigny, 1816) During Whole-Body Regeneration.
[So] Source:Biol Bull;232(3):143-157, 2017 Jun.
[Is] ISSN:1939-8697
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Whole-body regeneration (WBR)-the formation of an entire adult from only a small fragment of its own tissue-is extremely rare among chordates. Exceptionally, in the colonial ascidian Botrylloides leachii (Savigny, 1816) a fully functional adult is formed from their common vascular system after ablation of all adults from the colony in just 10 d, thanks to their high blastogenetic potential. While previous studies have identified key genetic markers and morphological changes, no study has yet focused on the hematological aspects of regeneration despite the major involvement of the remaining vascular system and the contained hemocytes in this process. To dissect this process, we analyzed colony blood flow patterns using time-lapse microscopy to obtain a quantitative description of the velocity, reversal pattern, and average distance traveled by hemocytes. We also observed that flows present during regeneration are powered by temporally and spatially synchronized contractions of the terminal ampullae. In addition, we revised previous studies of B. leachii hematology as well as asexual development using histological sectioning and compared the role played by hemocytes during WBR. We found that regeneration starts with a rapid healing response characterized by hemocyte aggregation and infiltration of immunocytes, followed by increased activity of hemoblasts, recruitment of macrophage-like cells for clearing the tissues of debris, and their subsequent disappearance from the circulation concomitant with the maturation of a single regenerated adult. Overall, we provide a detailed account of the hematological properties of regenerating B. leachii colonies, providing novel lines of inquiry toward the decipherment of regeneration in chordates.
[Mh] Termos MeSH primário: Regeneração/fisiologia
Urocordados/fisiologia
[Mh] Termos MeSH secundário: Animais
Hemócitos/metabolismo
Urocordados/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170913
[St] Status:MEDLINE
[do] DOI:10.1086/692841


  7 / 3486 MEDLINE  
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[PMID]:28877227
[Au] Autor:Hystad EM; Salmela H; Amdam GV; Münch D
[Ad] Endereço:Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Aas, Norway.
[Ti] Título:Hemocyte-mediated phagocytosis differs between honey bee (Apis mellifera) worker castes.
[So] Source:PLoS One;12(9):e0184108, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Honey bees as other insects rely on the innate immune system for protection against diseases. The innate immune system includes the circulating hemocytes (immune cells) that clear pathogens from hemolymph (blood) by phagocytosis, nodulation or encapsulation. Honey bee hemocyte numbers have been linked to hemolymph levels of vitellogenin. Vitellogenin is a multifunctional protein with immune-supportive functions identified in a range of species, including the honey bee. Hemocyte numbers can increase via mitosis, and this recruitment process can be important for immune system function and maintenance. Here, we tested if hemocyte mediated phagocytosis differs among the physiologically different honey bee worker castes (nurses, foragers and winter bees), and study possible interactions with vitellogenin and hemocyte recruitment. To this end, we adapted phagocytosis assays, which-together with confocal microscopy and flow cytometry-allow qualitative and quantitative assessment of hemocyte performance. We found that nurses are more efficient in phagocytic uptake than both foragers and winter bees. We detected vitellogenin within the hemocytes, and found that winter bees have the highest numbers of vitellogenin-positive hemocytes. Connections between phagocytosis, hemocyte-vitellogenin and mitosis were worker caste dependent. Our results demonstrate that the phagocytic performance of immune cells differs significantly between honey bee worker castes, and support increased immune competence in nurses as compared to forager bees. Our data, moreover, provides support for roles of vitellogenin in hemocyte activity.
[Mh] Termos MeSH primário: Abelhas/fisiologia
Hemócitos/fisiologia
Fagocitose/fisiologia
[Mh] Termos MeSH secundário: Animais
Abelhas/imunologia
Sistema Imunitário/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184108


  8 / 3486 MEDLINE  
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[PMID]:28867469
[Au] Autor:Kim SG; Jo YH; Seong JH; Park KB; Noh MY; Cho JH; Ko HJ; Kim CE; Tindwa H; Patnaik BB; Bang IS; Lee YS; Han YS
[Ad] Endereço:Division of Plant Biotechnology, Institute of Environmentally-Friendly Agriculture (IEFA), College of Agriculture and Life Sciences, Chonnam National University, Gwangju, 61186, Republic of Korea.
[Ti] Título:TmSR-C, scavenger receptor class C, plays a pivotal role in antifungal and antibacterial immunity in the coleopteran insect Tenebrio molitor.
[So] Source:Insect Biochem Mol Biol;89:31-42, 2017 Oct.
[Is] ISSN:1879-0240
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Scavenger receptors (SRs) constitute a family of membrane-bound receptors that bind to multiple ligands. The SR family of proteins is involved in removing cellular debris, oxidized low-density lipoproteins, and pathogens. Specifically, class C scavenger receptors (SR-C) have also been reported to be involved in phagocytosis of gram-positive and -negative bacteria in Drosophila and viruses in shrimp. However, reports are unavailable regarding the role of SR-C in antifungal immune mechanisms in insects. In this study, a full-length Tenebrio molitor SR-C (TmSR-C) sequence was obtained by 5'- and 3'-Rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR). The TmSR-C full-length cDNA comprised 1671 bp with 5'- and 3'-untranslated regions of 23- and 107-bp, respectively. TmSR-C encodes a putative protein of 556 amino acid residues that is constitutively expressed in all tissues of late instar larvae and 2-day-old adults, with the highest transcript levels observed in hemocytes of larvae and adults. TmSR-C mRNA showed a 2.5-fold and 3-fold increase at 24 and 6 h after infection with Candida albicans and ß-glucan, respectively. Immunoassay with TmSR-C polyclonal antibody showed induction of the putative protein in the cytosols of hemocytes at 3 h after inoculation of C. albicans. RNA interference (RNAi)-based gene silencing and phagocytosis assays were used to understand the role of TmSR-C in antifungal immunity. Silencing of TmSR-C transcripts reduced the survivability of late instar larvae at 2 days post-inoculation of C. albicans, Escherichia coli, or Staphylococcus aureus. Furthermore, in TmSR-C-silenced larvae, there was a decline in the rate of microorganism phagocytosis. Taken together, results of this study suggest that TmSR-C plays a pivotal role in phagocytosing not only fungi but also gram-negative and -positive bacteria in T. molitor.
[Mh] Termos MeSH primário: Fagocitose
Receptores Depuradores Classe C/fisiologia
Tenebrio/imunologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sequência de Bases
Candida albicans
Escherichia coli
Expressão Gênica
Hemócitos/metabolismo
Interferência de RNA
Análise de Sequência de DNA
Staphylococcus aureus
Tenebrio/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Scavenger Receptors, Class C)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170905
[St] Status:MEDLINE


  9 / 3486 MEDLINE  
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[PMID]:28844653
[Au] Autor:Vogelweith F; Moret Y; Thiéry D; Delbac L; Moreau J
[Ad] Endereço:Johannes-Gutenberg University of Mainz, Institute of Organismic and Molecular Evolution, Behavioral Ecology and Social Evolution Group, Johannes-von-Müller-Weg 6, 55128 Mainz, Germany. Electronic address: fanny.vogelweith@gmail.com.
[Ti] Título:No evidence of an immune adjustment in response to a parasitoid threat in Lobesia botrana larvae.
[So] Source:J Insect Physiol;102:7-11, 2017 Oct.
[Is] ISSN:1879-1611
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Immune function is a key determinant of an organism's fitness, and natural insect populations are highly variable for this trait, mainly due to environmental heterogeneity and pathogen diversity. We previously reported a positive correlation between infection prevalence by parasitoids and host immunity in natural populations of the vineyard pest Lobesia botrana. Here, we tested whether this correlation reflects a plastic adjustment of host immunity in response to the local presence of parasites. To this end, we measured immunity of non-parasitized L. botrana larvae exposed, respectively, to one of the two most common species of parasitoids in vineyards, over 6days. Larvae were able to sense the parasitoid through visual, chemical, or mechanical cues, but contact larvae-parasitoid were excluded. Contrary to our hypothesis, we found that L. botrana larvae did not increase their immune defenses in the presence of parasitoids, despite their ability to sense a potential threat. Our results therefore suggest that the positive correlation between infection prevalence by parasitoids and L. botrana immunity among natural populations may result from micro-evolutionary changes resulting from long-term local selection pressures imposed by parasitoids in wild populations rather than plastic adjustments of immunity.
[Mh] Termos MeSH primário: Mariposas/imunologia
Mariposas/parasitologia
Vespas/fisiologia
[Mh] Termos MeSH secundário: Animais
Catecol Oxidase/metabolismo
Precursores Enzimáticos/metabolismo
Hemócitos/metabolismo
Proteínas de Insetos/metabolismo
Larva/crescimento & desenvolvimento
Larva/imunologia
Larva/parasitologia
Larva/fisiologia
Mariposas/crescimento & desenvolvimento
Vespas/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Enzyme Precursors); 0 (Insect Proteins); EC 1.10.3.- (pro-phenoloxidase); EC 1.10.3.1 (Catechol Oxidase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171025
[Lr] Data última revisão:
171025
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170829
[St] Status:MEDLINE


  10 / 3486 MEDLINE  
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[PMID]:28792420
[Au] Autor:Ji A; Li X; Fang S; Qin Z; Bai C; Wang C; Zhang Z
[Ad] Endereço:Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao 266003, PR China.
[Ti] Título:Primary culture of Zhikong scallop Chlamys farreri hemocytes as an in vitro model for studying host-pathogen interactions.
[So] Source:Dis Aquat Organ;125(3):217-226, 2017 08 09.
[Is] ISSN:0177-5103
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Primary cultured cells can be a useful tool in studies on physiology, virology, and toxicology. Hemocytes play an important role in animal rapid response to pathogen invasion. In this study, an appropriate medium for primary culture of hemocytes of the bivalve Chlamys farreri was developed by adding 5% fetal bovine serum and 1% C. farreri serum to Leibovitz L-15 medium. These primary cultured hemocytes were maintained for more than 40 d in vitro and were classified into 3 types: (1) granulocytes containing numerous granules in the cytoplasm, (2) hyalinocytes with no or few granules, (3) a small percentage of macrophage-like cells. Furthermore, the primary cultured hemocytes were observed to be sensitive to bacterial and viral challenges. These hemocytes could phagocytose the bacterium Vibrio anguillarum, and presented cytopathic effects on the extracellular products (ECPs) of V. anguillarum; the mRNA level of QM, which plays an important role in immune response, also significantly increased 12 h after infection. When these hemocytes were challenged with ostreid herpesvirus 1 (OsHV-1), virus particles and empty capsids in the cells infected for 48 h were observed by transmission electron microscopy, and the QM mRNA level increased significantly at 12 h and 24 h following OsHV-1 challenge. This primary culture system is available for C. farreri hemocytes which can be used in the future to study host-pathogen interactions.
[Mh] Termos MeSH primário: Hemócitos/fisiologia
Herpesviridae/fisiologia
Pectinidae/citologia
Vibrio/fisiologia
[Mh] Termos MeSH secundário: Animais
Técnicas de Cultura de Células
Regulação da Expressão Gênica/imunologia
Hemócitos/microbiologia
Interações Hospedeiro-Patógeno
RNA Viral
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170810
[St] Status:MEDLINE
[do] DOI:10.3354/dao03145



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