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[PMID]:28464218
[Au] Autor:Köstlin N; Vogelmann M; Spring B; Schwarz J; Feucht J; Härtel C; Orlikowsky TW; Poets CF; Gille C
[Ad] Endereço:Department of Neonatology, Tübingen University Children's Hospital, Tübingen, Germany.
[Ti] Título:Granulocytic myeloid-derived suppressor cells from human cord blood modulate T-helper cell response towards an anti-inflammatory phenotype.
[So] Source:Immunology;152(1):89-101, 2017 09.
[Is] ISSN:1365-2567
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Infections are a leading cause of perinatal morbidity and mortality. The outstandingly high susceptibility to infections early in life is mainly attributable to the compromised state of the neonatal immune system. One important difference to the adult immune system is a bias towards T helper type 2 (Th2) responses in newborns. However, mechanisms regulating neonatal T-cell responses are incompletely understood. Granulocytic myeloid-derived suppressor cells (GR-MDSC) are myeloid cells with a granulocytic phenotype that suppress various functions of other immune cells and accumulate under physiological conditions during pregnancy in maternal and fetal blood. Although it has been hypothesized that GR-MDSC accumulation during fetal life could be important for the maintenance of maternal-fetal tolerance, the influence of GR-MDSC on the immunological phenotype of neonates is still unclear. Here, we investigated the impact of GR-MDSC isolated from cord blood (CB-MDSC) on the polarization of Th cells. We demonstrate that CB-MDSC inhibit Th1 responses and induced Th2 responses and regulatory T (Treg) cells. Th1 inhibition was cell-contact dependent and occurred independent of other cell types, while Th2 induction was mediated independently of cell contact through expression of ArgI and reactive oxygen species by CB-MDSC and partially needed the presence of monocytes. Treg cell induction by CB-MDSC also occurred cell-contact independently but was partially mediated through inducible nitric oxide synthase. These results point towards a role of MDSC in regulating neonatal immune responses. Targeting MDSC function in neonates could be a therapeutic opportunity to improve neonatal host defence.
[Mh] Termos MeSH primário: Plasticidade Celular
Sangue Fetal/imunologia
Granulócitos/imunologia
Inflamação/prevenção & controle
Células Supressoras Mieloides/imunologia
Células Th2/imunologia
[Mh] Termos MeSH secundário: Arginase/imunologia
Arginase/metabolismo
Comunicação Celular
Células Cultivadas
Técnicas de Cocultura
Sangue Fetal/citologia
Granulócitos/metabolismo
Seres Humanos
Recém-Nascido
Inflamação/imunologia
Inflamação/metabolismo
Células Supressoras Mieloides/metabolismo
Óxido Nítrico Sintase Tipo II/imunologia
Óxido Nítrico Sintase Tipo II/metabolismo
Fenótipo
Espécies Reativas de Oxigênio/imunologia
Espécies Reativas de Oxigênio/metabolismo
Transdução de Sinais
Linfócitos T Reguladores/imunologia
Linfócitos T Reguladores/metabolismo
Células Th1/imunologia
Células Th1/metabolismo
Células Th2/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Reactive Oxygen Species); EC 1.14.13.39 (NOS2 protein, human); EC 1.14.13.39 (Nitric Oxide Synthase Type II); EC 3.5.3.1 (Arginase); EC 3.5.3.1 (arginase I, human)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180131
[Lr] Data última revisão:
180131
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1111/imm.12751


  2 / 17262 MEDLINE  
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[PMID]:29300724
[Au] Autor:Draper JE; Sroczynska P; Fadlullah MZH; Patel R; Newton G; Breitwieser W; Kouskoff V; Lacaud G
[Ad] Endereço:Cancer Research UK Stem Cell Biology Group, Cancer Research UK Manchester Institute, Manchester Cancer Research Centre, The University of Manchester, Manchester, United Kingdom.
[Ti] Título:A novel prospective isolation of murine fetal liver progenitors to study in utero hematopoietic defects.
[So] Source:PLoS Genet;14(1):e1007127, 2018 01.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In recent years, highly detailed characterization of adult bone marrow (BM) myeloid progenitors has been achieved and, as a result, the impact of somatic defects on different hematopoietic lineage fate decisions can be precisely determined. Fetal liver (FL) hematopoietic progenitor cells (HPCs) are poorly characterized in comparison, potentially hindering the study of the impact of genetic alterations on midgestation hematopoiesis. Numerous disorders, for example infant acute leukemias, have in utero origins and their study would therefore benefit from the ability to isolate highly purified progenitor subsets. We previously demonstrated that a Runx1 distal promoter (P1)-GFP::proximal promoter (P2)-hCD4 dual-reporter mouse (Mus musculus) model can be used to identify adult BM progenitor subsets with distinct lineage preferences. In this study, we undertook the characterization of the expression of Runx1-P1-GFP and P2-hCD4 in FL. Expression of P2-hCD4 in the FL immunophenotypic Megakaryocyte-Erythroid Progenitor (MEP) and Common Myeloid Progenitor (CMP) compartments corresponded to increased granulocytic/monocytic/megakaryocytic and decreased erythroid specification. Moreover, Runx1-P2-hCD4 expression correlated with several endogenous cell surface markers' expression, including CD31 and CD45, providing a new strategy for prospective identification of highly purified fetal myeloid progenitors in transgenic mouse models. We utilized this methodology to compare the impact of the deletion of either total RUNX1 or RUNX1C alone and to determine the fetal HPCs lineages most substantially affected. This new prospective identification of FL progenitors therefore raises the prospect of identifying the underlying gene networks responsible with greater precision than previously possible.
[Mh] Termos MeSH primário: Linhagem da Célula/genética
Células-Tronco Hematopoéticas/citologia
Células Progenitoras Mieloides/citologia
[Mh] Termos MeSH secundário: Animais
Medula Óssea/embriologia
Diferenciação Celular
Subunidade alfa 2 de Fator de Ligação ao Core/genética
Subunidade alfa 2 de Fator de Ligação ao Core/metabolismo
Modelos Animais de Doenças
Granulócitos/citologia
Hematopoese/genética
Células-Tronco Hematopoéticas/metabolismo
Seres Humanos
Fígado/citologia
Fígado/embriologia
Fígado/metabolismo
Megacariócitos/citologia
Camundongos
Camundongos Transgênicos
Monócitos/citologia
Estudos Prospectivos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Core Binding Factor Alpha 2 Subunit); 0 (Runx1 protein, mouse)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180128
[Lr] Data última revisão:
180128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180105
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1007127


  3 / 17262 MEDLINE  
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[PMID]:29200148
[Au] Autor:Aoki T; Kunishima S; Yamashita Y; Minamitani K; Ota S
[Ad] Endereço:Department of Pediatrics, Teikyo University Chiba Medical Center, Chiba.
[Ti] Título:Macrothrombocytopenia With Congenital Bilateral Cataracts: A Phenotype of MYH9 Disorder With Exon 24 Indel Mutations.
[So] Source:J Pediatr Hematol Oncol;40(1):76-78, 2018 Jan.
[Is] ISSN:1536-3678
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:MYH9 disorder is characterized by large platelets and granulocyte inclusion bodies, and can be complicated with young-adult onsets of nephropathy, sensorineural hearing loss, and cataracts. Congenital cataracts in patients with MYH9 disorder is rare, and their etiology has not been elucidated. We report a 3-year-old patient with MYH9 disorder who had a p.E1066_A1072del mutation and developed cataracts congenitally. A review of the literature reveals that patients with an MYH9 exon 24 indel mutation, including p.E1066_A1072del, are susceptible to developing congenital cataracts and should be followed closely for other nonhematological complications.
[Mh] Termos MeSH primário: Catarata/congênito
Granulócitos/ultraestrutura
Mutação INDEL
Proteínas Motores Moleculares/genética
Cadeias Pesadas de Miosina/genética
Trombocitopenia/complicações
[Mh] Termos MeSH secundário: Plaquetas/patologia
Catarata/genética
Pré-Escolar
Éxons
Granulócitos/patologia
Perda Auditiva Neurossensorial
Seres Humanos
Corpos de Inclusão/patologia
Fenótipo
Trombocitopenia/congênito
Trombocitopenia/etiologia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MYH9 protein, human); 0 (Molecular Motor Proteins); EC 3.6.4.1 (Myosin Heavy Chains)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180112
[Lr] Data última revisão:
180112
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171205
[St] Status:MEDLINE
[do] DOI:10.1097/MPH.0000000000000998


  4 / 17262 MEDLINE  
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[PMID]:29215832
[Au] Autor:Maklakova IY; Grebnev DY; Yastrebov AP
[Ti] Título:[Activation of regeneration of red and white pulp of the spleen after the combined transplantation of HSC and MSCS in terms of exposure to ionizing radiation].
[So] Source:Patol Fiziol Eksp Ter;61(2):22-7, 2017 Apr-Jun.
[Is] ISSN:0031-2991
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The purpose of this work was to study the effect of combined transplantation of multipotent mesenchymal stromal (MSCS) and hematopoietic stem cells (HSCs) isolated from the placenta, on the regeneration of white and red pulp of the spleen under physiological conditions and in conditions of exposure to ionizing radiation. Methods. The experiments were performed with laboratory mice-males. We studied the influence of ionizing radiation dose of 4.0 Gy. Animals of the experimental group were intravenously infused into MMSC and GSK respectively at a dose of 6 million cells/kg and 330 thousand cells/kg, suspended in 0.2 ml of 0.9% NaCl solution. The selection of hematopoietic stem cells was carried out using the direct technique of immune magnetic separation. Were studied the following morphometric parameters of the spleen: the average area of lymphoid follicles, the average area of zone of lymphoid follicles, average size of germinal center of lymphoid follicles, average size T-zones of lymphoid follicles, the average distance between the centers of the follicles, the average cellularity of the red pulp. Results. As a result, of research obtained that after exposure to ionizing radiation on the background of combined transplantation of HSC and MSCS there is an increase in size of lymphoid follicle at the expense of area B-zone of the follicle, the area germinative center of the follicle, restoring the content of lymphoblasts and lymphoblasts and lymphocytes to normal values. On the background of transplantation MMSC and GSK in terms of radiation exposure changes and the red pulp of the spleen. The increase in the density of cells in the red pulp of the spleen and, as a consequence, of the increase of the distance between the centers of lymphoid follicles. The increase in the density of cells in the red pulp occurs due to the increase in the content of erythroid cells and by increasing granulocytes. Key words: ionizing radiation, multipotent mesenchymal stromal cells, hematopoietic stem cells, spleen, regeneration. Conclusion. Studies have shown the effectiveness of combined transplantation MSC and GSK in respect of the main morphometric parameters of the spleen after exposure to ionizing radiation.
[Mh] Termos MeSH primário: Granulócitos/metabolismo
Transplante de Células-Tronco Hematopoéticas
Transplante de Células-Tronco Mesenquimais
Regeneração
Baço/fisiologia
[Mh] Termos MeSH secundário: Aloenxertos
Animais
Células Eritroides/metabolismo
Células Eritroides/patologia
Granulócitos/patologia
Masculino
Camundongos
Baço/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171221
[Lr] Data última revisão:
171221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171208
[St] Status:MEDLINE


  5 / 17262 MEDLINE  
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[PMID]:28749083
[Au] Autor:Menegat L; Simas R; Caliman JM; Zanoni FL; Jacysyn JF; da Silva LFF; Borelli P; Moreira LFP; Sannomiya P
[Ad] Endereço:Heart Institute (InCor), LIM 11, University of São Paulo Medical School, São Paulo, SP, Brazil.
[Ti] Título:Evidence of bone marrow downregulation in brain-dead rats.
[So] Source:Int J Exp Pathol;98(3):158-165, 2017 06.
[Is] ISSN:1365-2613
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Experimental findings support the evidence of a persistent leucopenia triggered by brain death (BD). This study aimed to investigate leucocyte behaviour in bone marrow and blood after BD in rats. BD was induced using intracranial balloon catheter inflation. Sham-operated (SH) rats were trepanned only. Thereafter bone marrow cells were harvested every six hours from the femoral cavity and used for total and differential counts. They were analysed further by flow cytometry to characterize lymphocyte subsets, granulocyte adhesion molecules expression and apoptosis/necrosis [annexin V/propidium iodide (PI) protocol]. BD rats exhibited a reduction in bone marrow cells due to a reduction in lymphocytes (40%) and segmented cells (45%). Bone marrow lymphocyte subsets were similar in BD and SH rats (CD3, P = 0.1; CD4, P = 0.4; CD3/CD4, P = 0.4; CD5, P = 0.4, CD3/CD5, P = 0.2; CD8, P = 0.8). Expression of L-selectin and beta -integrins on granulocytes did not differ (CD11a, P = 0.9; CD11b/c, P = 0.7; CD62L, P = 0.1). There were no differences in the percentage of apoptosis and necrosis (Annexin V, P = 0.73; PI, P = 0.21; Annexin V/PI, P = 0.29). In conclusion, data presented suggest that the downregulation of the bone marrow is triggered by brain death itself, and it is not related to changes in lymphocyte subsets, granulocyte adhesion molecules expression or apoptosis and necrosis.
[Mh] Termos MeSH primário: Células da Medula Óssea/patologia
Morte Encefálica/patologia
[Mh] Termos MeSH secundário: Animais
Apoptose
Células da Medula Óssea/imunologia
Células da Medula Óssea/metabolismo
Morte Encefálica/imunologia
Morte Encefálica/metabolismo
Moléculas de Adesão Celular/metabolismo
Modelos Animais de Doenças
Regulação para Baixo
Granulócitos/metabolismo
Hemodinâmica/fisiologia
Contagem de Leucócitos
Leucopenia/etiologia
Subpopulações de Linfócitos/imunologia
Masculino
Necrose
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cell Adhesion Molecules)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171201
[Lr] Data última revisão:
171201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1111/iep.12234


  6 / 17262 MEDLINE  
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[PMID]:28976973
[Au] Autor:Qin Y; Wu L; Ouyang Y; Zhou P; Zhou H; Wang Y; Ma J; Zhang J; Chen Y; Qian J; Tang Y; Shen N
[Ad] Endereço:Department of Rheumatology and Shanghai Institute of Rheumatology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
[Ti] Título:MiR-125a Is a critical modulator for neutrophil development.
[So] Source:PLoS Genet;13(10):e1007027, 2017 Oct.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:MicroRNAs are universal post-transcriptional regulators in genomes. They have the ability of buffering gene expressional programs, contributing to robustness of biological systems and playing important roles in development, physiology and diseases. Here, we identified a microRNA, miR-125a, as a positive regulator of granulopoiesis. MiR125a knockout mice show reduced infiltration of neutrophils in the lung and alleviated tissue destruction after endotoxin challenge as a consequence of decreased neutrophil numbers. Furthermore, we demonstrated that this significant reduction of neutrophils was due to impaired development of granulocyte precursors to mature neutrophils in an intrinsic manner. We showed that Socs3, a critical repressor for granulopoiesis, was a target of miR-125a. Overall, our study revealed a new microRNA regulating granulocyte development and supported a model in which miR-125a acted as a fine-tuner of granulopoiesis.
[Mh] Termos MeSH primário: Leucopoese/genética
Leucopoese/fisiologia
MicroRNAs/genética
MicroRNAs/metabolismo
Neutrófilos/citologia
Neutrófilos/metabolismo
[Mh] Termos MeSH secundário: Regiões 3' não Traduzidas
Animais
Sítios de Ligação/genética
Morte Celular
Diferenciação Celular
Proliferação Celular
Fator Estimulador de Colônias de Granulócitos/metabolismo
Granulócitos/citologia
Granulócitos/metabolismo
Camundongos
Camundongos Knockout
Modelos Biológicos
Células Progenitoras Mieloides/citologia
Células Progenitoras Mieloides/metabolismo
Choque Séptico/genética
Choque Séptico/metabolismo
Choque Séptico/patologia
Transdução de Sinais
Proteína 3 Supressora da Sinalização de Citocinas/genética
Proteína 3 Supressora da Sinalização de Citocinas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (3' Untranslated Regions); 0 (MicroRNAs); 0 (Mirn125 microRNA, mouse); 0 (Socs3 protein, mouse); 0 (Suppressor of Cytokine Signaling 3 Protein); 143011-72-7 (Granulocyte Colony-Stimulating Factor)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171005
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1007027


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[PMID]:28947270
[Au] Autor:Ito R; Nagai D; Igo N; Okuda Y; Sekine K; Ichimura E; Katano I; Mizushima T; Goto M; Ohnishi Y; Ito M; Okamoto K
[Ad] Endereço:Central Institute for Experimental Animals, 3-25-12 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-0821, Japan.
[Ti] Título:A novel in vivo model for predicting myelotoxicity of chemotherapeutic agents using IL-3/GM-CSF transgenic humanized mice.
[So] Source:Toxicol Lett;281:152-157, 2017 Nov 05.
[Is] ISSN:1879-3169
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Evaluating myelotoxicity is essential for ensuring the safety of novel drugs before they are approved for clinical applications. Although in vivo prediction of the maximum tolerated doses (MTDs) of anticancer drugs is usually performed in rodents, the results are not always applicable to clinical treatment because drugs may have different effects in human and rodent cells. Previously, we generated a human IL-3 and GM-CSF transgenic humanized mouse (hu-IL-3/GM Tg), in which human granulocytes effectively differentiated after hematopoietic stem cell transplantation. In this study, we established a novel in vivo preclinical evaluation model for predicting human myelotoxicity of anticancer drugs using these hu-IL-3/GM Tg mice. The myelotoxicity was investigated by kinetic flow cytometry of human or murine granulocytes and by colony-forming unit granulocyte/macrophage (CFU-GM) assays. In both in vivo and in vitro analyses, topotecan was more myelotoxic to human than murine granulocytes. In contrast, oxaliplatin was more myelotoxic to murine granulocytes. The level of myelotoxicity of paclitaxel treatment was comparable between human and mouse cells. These results demonstrate that our humanized mouse model can simultaneously evaluate myelotoxicity against human and mouse cells in vivo, and provides an effective preclinical tool for predicting appropriate doses of anticancer agents for clinical treatment.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/toxicidade
Paclitaxel/toxicidade
[Mh] Termos MeSH secundário: Animais
Diferenciação Celular/efeitos dos fármacos
Linhagem Celular Tumoral
Ensaio de Unidades Formadoras de Colônias
Relação Dose-Resposta a Droga
Fator Estimulador de Colônias de Granulócitos e Macrófagos/genética
Granulócitos/efeitos dos fármacos
Células-Tronco Hematopoéticas/efeitos dos fármacos
Seres Humanos
Concentração Inibidora 50
Interleucina-3/genética
Camundongos
Camundongos Endogâmicos NOD
Camundongos Transgênicos
Testes de Toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Interleukin-3); 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor); P88XT4IS4D (Paclitaxel)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170927
[St] Status:MEDLINE


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[PMID]:28922396
[Au] Autor:Sasaki F; Koga T; Saeki K; Okuno T; Kazuno S; Fujimura T; Ohkawa Y; Yokomizo T
[Ad] Endereço:Department of Biochemistry, Juntendo University School of Medicine, Tokyo, Japan.
[Ti] Título:Biochemical and immunological characterization of a novel monoclonal antibody against mouse leukotriene B4 receptor 1.
[So] Source:PLoS One;12(9):e0185133, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Leukotriene B4 (LTB4) receptor 1 (BLT1) is a G protein-coupled receptor expressed in various leukocyte subsets; however, the precise expression of mouse BLT1 (mBLT1) has not been reported because a mBLT1 monoclonal antibody (mAb) has not been available. In this study, we present the successful establishment of a hybridoma cell line (clone 7A8) that produces a high-affinity mAb for mBLT1 by direct immunization of BLT1-deficient mice with mBLT1-overexpressing cells. The specificity of clone 7A8 was confirmed using mBLT1-overexpressing cells and mouse peripheral blood leukocytes that endogenously express BLT1. Clone 7A8 did not cross-react with human BLT1 or other G protein-coupled receptors, including human chemokine (C-X-C motif) receptor 4. The 7A8 mAb binds to the second extracellular loop of mBLT1 and did not affect LTB4 binding or intracellular calcium mobilization by LTB4. The 7A8 mAb positively stained Gr-1-positive granulocytes, CD11b-positive granulocytes/monocytes, F4/80-positive monocytes, CCR2-high and CCR2-low monocyte subsets in the peripheral blood and a CD4-positive T cell subset, Th1 cells differentiated in vitro from naïve CD4-positive T cells. This mAb was able to detect Gr-1-positive granulocytes and monocytes in the spleens of naïve mice by immunohistochemistry. Finally, intraperitoneal administration of 7A8 mAb depleted granulocytes and monocytes in the peripheral blood. We have therefore succeeded in generating a high-affinity anti-mBLT1 mAb that is useful for analyzing mBLT1 expression in vitro and in vivo.
[Mh] Termos MeSH primário: Anticorpos Monoclonais Murinos/imunologia
Leucotrieno B4/imunologia
Receptores do Leucotrieno B4/antagonistas & inibidores
[Mh] Termos MeSH secundário: Animais
Anticorpos Monoclonais Murinos/química
Anticorpos Monoclonais Murinos/farmacologia
Células CHO
Sinalização do Cálcio/efeitos dos fármacos
Diferenciação Celular/imunologia
Cricetinae
Cricetulus
Granulócitos/imunologia
Masculino
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Knockout
Monócitos/imunologia
Estrutura Secundária de Proteína
Receptores do Leucotrieno B4/química
Receptores do Leucotrieno B4/imunologia
Células Th1/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Monoclonal, Murine-Derived); 0 (Ltb4r1 protein, mouse); 0 (Receptors, Leukotriene B4); 1HGW4DR56D (Leukotriene B4)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170919
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185133


  9 / 17262 MEDLINE  
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[PMID]:28815621
[Au] Autor:Lodermeier MA; Byrne KM; Flegel WA
[Ad] Endereço:Department of Transfusion Medicine, National Institutes of Health Clinical Center, National Institutes of Health, Bethesda, Maryland.
[Ti] Título:Red blood cell sedimentation of Apheresis Granulocytes.
[So] Source:Transfusion;57(10):2551-2552, 2017 Oct.
[Is] ISSN:1537-2995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sedimentation of Apheresis Granulocyte components removes red blood cells. It is used to increase the blood donor pool when blood group-compatible donors cannot be recruited for a patient because of a major ABO incompatibility or incompatible red blood cell antibodies in the recipient. Because granulocytes have little ABO and few other red blood cell antigens on their membrane, such incompatibility lies mostly with the contaminating red blood cells. Video Clip S1 shows the process of red blood cell sedimentation of an Apheresis Granulocyte component. This video was filmed with a single smart phone attached to a commercial tripod and was edited on a tablet computer with free software by an amateur videographer without prior video experience.
[Mh] Termos MeSH primário: Sedimentação Sanguínea
Granulócitos/citologia
Gravação em Vídeo/instrumentação
[Mh] Termos MeSH secundário: Remoção de Componentes Sanguíneos
Doadores de Sangue/provisão & distribuição
Incompatibilidade de Grupos Sanguíneos
Seres Humanos
Smartphone
Software
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170818
[St] Status:MEDLINE
[do] DOI:10.1111/trf.14251


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[PMID]:28791704
[Au] Autor:Mulcahy MJ; Lester HA
[Ad] Endereço:Department of Biology, California Institute of Technology, Pasadena, California, USA.
[Ti] Título:Granulocytes as models for human protein marker identification following nicotine exposure.
[So] Source:J Neurochem;142 Suppl 2:151-161, 2017 Aug.
[Is] ISSN:1471-4159
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Nicotinic acetylcholine receptors (nAChRs) are pentameric cation channels expressed in the mammalian CNS, in the peripheral nervous system, and in skeletal muscle. Neuronal-type nAChRs are also found in several non-neuronal cell types, including leukocytes. Granulocytes are a subtype of leukocytes that include basophils, eosinophils, and neutrophils. Granulocytes, also known as polymorphonuclear leukocytes, are characterized by their ability to produce, store, and release compounds from intracellular granules. Granulocytes are the most abundant type of leukocyte circulating in the peripheral blood. Granulocyte abundance, nAChR expression, and nAChR upregulation following chronic nicotine administration makes granulocytes interesting models for identifying protein markers of nicotine exposure. Nicotinic receptor subunits and several non-nAChR proteins have been identified as protein markers of granulocyte nicotine exposure. We review methods to isolate granulocytes from human tissue, summarize present data about the expression of nAChRs in the three granulocyte cell types (basophils, eosinophils, and neutrophils), describe current knowledge of the effects of nicotine exposure on human granulocyte protein expression, and highlight areas of interest for future investigation. This is an article for the special issue XVth International Symposium on Cholinergic Mechanisms.
[Mh] Termos MeSH primário: Granulócitos/efeitos dos fármacos
Nicotina/farmacologia
Agonistas Nicotínicos/farmacologia
RNA Mensageiro/efeitos dos fármacos
Receptores Nicotínicos/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Granulócitos/metabolismo
Seres Humanos
Piridinas/farmacologia
RNA Mensageiro/metabolismo
Receptores Nicotínicos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Nicotinic Agonists); 0 (Pyridines); 0 (RNA, Messenger); 0 (Receptors, Nicotinic); 6M3C89ZY6R (Nicotine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170810
[St] Status:MEDLINE
[do] DOI:10.1111/jnc.14010



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