Base de dados : MEDLINE
Pesquisa : A11.148.350.350 [Categoria DeCS]
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[PMID]:28605567
[Au] Autor:Xu Z; Ji J; Xu J; Li D; Shi G; Liu F; Ding L; Ren J; Dou H; Wang T; Hou Y
[Ad] Endereço:The State Key Laboratory of Pharmaceutical Biotechnology, Division of Immunology, Medical School, Nanjing University, Nanjing, China.
[Ti] Título:MiR-30a increases MDSC differentiation and immunosuppressive function by targeting SOCS3 in mice with B-cell lymphoma.
[So] Source:FEBS J;284(15):2410-2424, 2017 Aug.
[Is] ISSN:1742-4658
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Myeloid-derived suppressor cells (MDSCs), including granulocytic (G)-MDSCs and monocytic (M)-MDSCs, play a critical role in tumor-induced T cell tolerance. MDSC immunosuppressive function and differentiation are significantly promoted in patients and B-cell lymphoma model mice. However, the mechanisms regulating these processes remain largely unclear. In the present study, we observed increased microRNA (miR)-30a expression both in G-MDSCs and in M-MDSCs from B cell lymphoma model mice. After transfection with miR-30a mimics, the differentiation and suppressive capacities of MDSCs were significantly increased via up-regulation of arginase-1. Moreover, we showed that the 3'-UTR of suppressor of cytokine signaling 3 (SOCS3) mRNA is a direct target of miR-30a. Decreased SOCS3 expression and activated Janus kinase-signal transducer and activator of transcription 3 signaling promote MDSC differentiation and suppressive activities. These findings provide new insights into the molecular mechanisms underlying MDSC expansion and function during B cell lymphoma development.
[Mh] Termos MeSH primário: Regiões 3´ não Traduzidas
Diferenciação Celular
Linfoma de Células B/metabolismo
MicroRNAs/metabolismo
Células Supressoras Mieloides/metabolismo
Proteína 3 Supressora da Sinalização de Citocinas/metabolismo
Regulação para Cima
[Mh] Termos MeSH secundário: Animais
Arginase/genética
Arginase/metabolismo
Células da Medula Óssea/patologia
Linfócitos T CD4-Positivos/imunologia
Linfócitos T CD4-Positivos/metabolismo
Linfócitos T CD4-Positivos/patologia
Linhagem Celular Tumoral
Células Cultivadas
Regulação Neoplásica da Expressão Gênica
Células Precursoras de Granulócitos/imunologia
Células Precursoras de Granulócitos/metabolismo
Células Precursoras de Granulócitos/patologia
Imunossupressão
Linfoma de Células B/imunologia
Linfoma de Células B/patologia
Linfoma de Células B/terapia
Camundongos
Camundongos Endogâmicos BALB C
MicroRNAs/antagonistas & inibidores
Células Precursoras de Monócitos e Macrófagos/imunologia
Células Precursoras de Monócitos e Macrófagos/metabolismo
Células Precursoras de Monócitos e Macrófagos/patologia
Células Supressoras Mieloides/citologia
Células Supressoras Mieloides/imunologia
Células Supressoras Mieloides/patologia
Proteínas de Neoplasias/genética
Proteínas de Neoplasias/metabolismo
Transplante de Neoplasias
Baço/patologia
Proteína 3 Supressora da Sinalização de Citocinas/genética
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (3' Untranslated Regions); 0 (MicroRNAs); 0 (Mirn30d microRNA, mouse); 0 (Neoplasm Proteins); 0 (Socs3 protein, mouse); 0 (Suppressor of Cytokine Signaling 3 Protein); EC 3.5.3.1 (Arg1 protein, mouse); EC 3.5.3.1 (Arginase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170613
[St] Status:MEDLINE
[do] DOI:10.1111/febs.14133


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[PMID]:28374668
[Au] Autor:Smetana K; Mikulenková D; Klamová H
[Ad] Endereço:Institute of Haematology and Blood Transfusion, Prague, Czech Republic.
[Ti] Título:Morphometric and Densitometric Analysis of Heterochromatin during Cell Differentiation Using the Leukaemic Granulocytic Lineage as a Convenient Model.
[So] Source:Folia Biol (Praha);63(1):1-5, 2017.
[Is] ISSN:0015-5500
[Cp] País de publicação:Czech Republic
[La] Idioma:eng
[Ab] Resumo:Granulocytic early progenitors and terminally differentiated - mature granulocytes with segmented nuclei were studied using computer-assisted diameter and heterochromatin optical image densitometry to provide more information on the nuclear size and heterochromatin condensation state. Bone marrow smears of patients suffering from chronic myeloid leukaemia untreated as well as treated with "specific" anti-leukaemic therapy with imatinib mesylate are a convenient model for such study because they possess a satisfactory number of cells for diameter and optical density measurements. In addition, the identification of developmental stages of granulocytes is very easy and the morphology is not different from that in not-leukaemic persons. As it was expected, the mean diameter of nuclear segments in fully differentiated and mature granulocytes was much smaller than that in non-segmented nuclei of early granulocytic precursors. Therefore, no wonder that the heterochromatin condensation state in nuclear segments of mature granulocytes was much larger than in non-segmented nuclei of granulocytic progenitors. On the other hand, the sum of mean diameters of all nuclear segments per cell was close to the mean nuclear diameter of early granulocytic progenitors. The heterochromatin condensation state in granulocytic progenitors or fully differentiated mature granulocytes exhibited marked stability and did not change after the anti-leukaemic therapy. In addition, Barr bodies of characteristic drumstick appearance bearing inactive X chromosome in interphase nuclei of mature granulocytes in fertile female patients exhibited a heterochromatin condensation state similar to nuclear segments. This heterochromatin condensation state was also stable and constant, and was not apparently influenced by the anti-leukaemic therapy.
[Mh] Termos MeSH primário: Diferenciação Celular
Linhagem da Célula
Densitometria/métodos
Granulócitos/patologia
Heterocromatina/química
Leucemia Mielogênica Crônica BCR-ABL Positiva/patologia
[Mh] Termos MeSH secundário: Feminino
Células Precursoras de Granulócitos/patologia
Seres Humanos
Modelos Biológicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Heterochromatin)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170719
[Lr] Data última revisão:
170719
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170405
[St] Status:MEDLINE


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[PMID]:28356386
[Au] Autor:Netherby CS; Messmer MN; Burkard-Mandel L; Colligan S; Miller A; Cortes Gomez E; Wang J; Nemeth MJ; Abrams SI
[Ad] Endereço:Department of Immunology, Roswell Park Cancer Institute, Buffalo, NY 14263.
[Ti] Título:The Granulocyte Progenitor Stage Is a Key Target of IRF8-Mediated Regulation of Myeloid-Derived Suppressor Cell Production.
[So] Source:J Immunol;198(10):4129-4139, 2017 May 15.
[Is] ISSN:1550-6606
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Alterations in myelopoiesis are common across various tumor types, resulting in immature populations termed myeloid-derived suppressor cells (MDSCs). MDSC burden correlates with poorer clinical outcomes, credited to their ability to suppress antitumor immunity. MDSCs consist of two major subsets, monocytic and polymorphonuclear (PMN). Intriguingly, the latter subset predominates in many patients and tumor models, although the mechanisms favoring PMN-MDSC responses remain poorly understood. Ordinarily, lineage-restricted transcription factors regulate myelopoiesis that collectively dictate cell fate. One integral player is IFN regulatory factor (IRF)-8, which promotes monocyte/dendritic cell differentiation while limiting granulocyte development. We recently showed that IRF8 inversely controls MDSC burden in tumor models, particularly the PMN-MDSC subset. However, where IRF8 acts in the pathway of myeloid differentiation to influence PMN-MDSC production has remained unknown. In this study, we showed that: 1) tumor growth was associated with a selective expansion of newly defined IRF8 granulocyte progenitors (GPs); 2) tumor-derived GPs had an increased ability to form PMN-MDSCs; 3) tumor-derived GPs shared gene expression patterns with IRF8 GPs, suggesting that IRF8 loss underlies GP expansion; and 4) enforced IRF8 overexpression in vivo selectively constrained tumor-induced GP expansion. These findings support the hypothesis that PMN-MDSCs result from selective expansion of IRF8 GPs, and that strategies targeting IRF8 expression may limit their load to improve immunotherapy efficacy.
[Mh] Termos MeSH primário: Células Precursoras de Granulócitos/fisiologia
Fatores Reguladores de Interferon/metabolismo
Neoplasias Mamárias Experimentais/imunologia
Neoplasias Mamárias Experimentais/fisiopatologia
Células Supressoras Mieloides/fisiologia
Mielopoese
[Mh] Termos MeSH secundário: Animais
Diferenciação Celular
Proliferação Celular
Feminino
Regulação Neoplásica da Expressão Gênica
Células Precursoras de Granulócitos/imunologia
Granulócitos/imunologia
Hematopoese
Seres Humanos
Fatores Reguladores de Interferon/genética
Camundongos
Monócitos/imunologia
Células Mieloides/imunologia
Células Supressoras Mieloides/imunologia
Neutrófilos/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Interferon Regulatory Factors); 0 (interferon regulatory factor-8)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170331
[St] Status:MEDLINE
[do] DOI:10.4049/jimmunol.1601722


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[PMID]:28235862
[Au] Autor:Zhu H; Kwak HJ; Liu P; Bajrami B; Xu Y; Park SY; Nombela-Arrieta C; Mondal S; Kambara H; Yu H; Chai L; Silberstein LE; Cheng T; Luo HR
[Ad] Endereço:The State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China.
[Ti] Título:Reactive Oxygen Species-Producing Myeloid Cells Act as a Bone Marrow Niche for Sterile Inflammation-Induced Reactive Granulopoiesis.
[So] Source:J Immunol;198(7):2854-2864, 2017 Apr 01.
[Is] ISSN:1550-6606
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Both microbial infection and sterile inflammation augment bone marrow (BM) neutrophil production, but whether the induced accelerated granulopoiesis is mediated by a common pathway and the nature of such a pathway are poorly defined. We recently established that BM myeloid cell-derived reactive oxygen species (ROS) externally regulate myeloid progenitor proliferation and differentiation in bacteria-elicited emergency granulopoiesis. In this article, we show that BM ROS levels are also elevated during sterile inflammation. Similar to in microbial infection, ROS were mainly generated by the phagocytic NADPH oxidase in Gr1 myeloid cells. The myeloid cells and their ROS were uniformly distributed in the BM when visualized by multiphoton intravital microscopy, and ROS production was both required and sufficient for sterile inflammation-elicited reactive granulopoiesis. Elevated granulopoiesis was mediated by ROS-induced phosphatase and tensin homolog oxidation and deactivation, leading to upregulated PtdIns(3,4,5)P3 signaling and increased progenitor cell proliferation. Collectively, these results demonstrate that, although infection-induced emergency granulopoiesis and sterile inflammation-elicited reactive granulopoiesis are triggered by different stimuli and are mediated by distinct upstream signals, the pathways converge to NADPH oxidase-dependent ROS production by BM myeloid cells. Thus, BM Gr1 myeloid cells represent a key hematopoietic niche that supports accelerated granulopoiesis in infective and sterile inflammation. This niche may be an excellent target in various immune-mediated pathologies or immune reconstitution after BM transplantation.
[Mh] Termos MeSH primário: Células Precursoras de Granulócitos/metabolismo
Granulócitos/metabolismo
Hematopoese/imunologia
Inflamação/metabolismo
Espécies Reativas de Oxigênio/metabolismo
[Mh] Termos MeSH secundário: Animais
Western Blotting
Diferenciação Celular/imunologia
Separação Celular
Modelos Animais de Doenças
Citometria de Fluxo
Granulócitos/citologia
Hematopoese/fisiologia
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Mutantes
Microscopia Confocal
Células Mieloides/citologia
Células Mieloides/metabolismo
Nicho de Células-Tronco/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Reactive Oxygen Species)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170815
[Lr] Data última revisão:
170815
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170226
[St] Status:MEDLINE
[do] DOI:10.4049/jimmunol.1602006


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[PMID]:28179497
[Au] Autor:Blazkova J; Gupta S; Liu Y; Gaudilliere B; Ganio EA; Bolen CR; Saar-Dover R; Fragiadakis GK; Angst MS; Hasni S; Aghaeepour N; Stevenson D; Baldwin N; Anguiano E; Chaussabel D; Altman MC; Kaplan MJ; Davis MM; Furman D
[Ad] Endereço:Division of Translational Medicine, Department of Systems Biology, Sidra Medical and Research Center, 26999 Doha, Qatar.
[Ti] Título:Multicenter Systems Analysis of Human Blood Reveals Immature Neutrophils in Males and During Pregnancy.
[So] Source:J Immunol;198(6):2479-2488, 2017 Mar 15.
[Is] ISSN:1550-6606
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Despite clear differences in immune system responses and in the prevalence of autoimmune diseases between males and females, there is little understanding of the processes involved. In this study, we identified a gene signature of immature-like neutrophils, characterized by the overexpression of genes encoding for several granule-containing proteins, which was found at higher levels (up to 3-fold) in young (20-30 y old) but not older (60 to >89 y old) males compared with females. Functional and phenotypic characterization of peripheral blood neutrophils revealed more mature and responsive neutrophils in young females, which also exhibited an elevated capacity in neutrophil extracellular trap formation at baseline and upon microbial or sterile autoimmune stimuli. The expression levels of the immature-like neutrophil signature increased linearly with pregnancy, an immune state of increased susceptibility to certain infections. Using mass cytometry, we also find increased frequencies of immature forms of neutrophils in the blood of women during late pregnancy. Thus, our findings show novel sex differences in innate immunity and identify a common neutrophil signature in males and in pregnant women.
[Mh] Termos MeSH primário: Fatores Etários
Células Sanguíneas/fisiologia
Células Precursoras de Granulócitos/fisiologia
Neutrófilos/fisiologia
Sexo
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Feminino
Seres Humanos
Masculino
Meia-Idade
Gravidez
Transcriptoma
Adulto Jovem
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; MULTICENTER STUDY
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170210
[St] Status:MEDLINE
[do] DOI:10.4049/jimmunol.1601855


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[PMID]:28159742
[Au] Autor:Endele M; Loeffler D; Kokkaliaris KD; Hilsenbeck O; Skylaki S; Hoppe PS; Schambach A; Stanley ER; Schroeder T
[Ad] Endereço:Research Unit Stem Cell Dynamics, Helmholtz Center Munich-German Research Center for Environmental Health, Neuherberg, Germany.
[Ti] Título:CSF-1-induced Src signaling can instruct monocytic lineage choice.
[So] Source:Blood;129(12):1691-1701, 2017 Mar 23.
[Is] ISSN:1528-0020
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Controlled regulation of lineage decisions is imperative for hematopoiesis. Yet, the molecular mechanisms underlying hematopoietic lineage choices are poorly defined. Colony-stimulating factor 1 (CSF-1), the cytokine acting as the principal regulator of monocyte/macrophage (M) development, has been shown to be able to instruct the lineage choice of uncommitted granulocyte M (GM) progenitors toward an M fate. However, the intracellular signaling pathways involved are unknown. CSF-1 activates a multitude of signaling pathways resulting in a pleiotropic cellular response. The precise role of individual pathways within this complex and redundant signaling network is dependent on cellular context, and is not well understood. Here, we address which CSF-1-activated pathways are involved in transmitting the lineage-instructive signal in primary bone marrow-derived GM progenitors. Although its loss is compensated for by alternative signaling activation mechanisms, Src family kinase (SFK) signaling is sufficient to transmit the CSF-1 lineage instructive signal. Moreover, c-Src activity is sufficient to drive M fate, even in nonmyeloid cells.
[Mh] Termos MeSH primário: Linhagem da Célula
Fator Estimulador de Colônias de Macrófagos/fisiologia
Monócitos/citologia
Transdução de Sinais
Quinases da Família src/metabolismo
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Células Precursoras de Granulócitos/citologia
Hematopoese
Camundongos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
81627-83-0 (Macrophage Colony-Stimulating Factor); EC 2.7.10.2 (src-Family Kinases)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170823
[Lr] Data última revisão:
170823
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170205
[St] Status:MEDLINE
[do] DOI:10.1182/blood-2016-05-714329


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[PMID]:28108472
[Au] Autor:Dayton VJ; McKenna RW; Yohe SL; Dolan MM; Courville E; Alvarez H; Linden MA
[Ad] Endereço:From the Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis.
[Ti] Título:Relapsed Acute Promyelocytic Leukemia Lacks "Classic" Leukemic Promyelocyte Morphology and Can Create Diagnostic Challenges.
[So] Source:Am J Clin Pathol;147(1):69-76, 2017 Jan 01.
[Is] ISSN:1943-7722
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Objectives: Although current therapies for acute promyelocytic leukemia (APL), such as all- trans retinoic acid and arsenic trioxide, usually result in remission, some patients relapse. Early recognition of relapse is critical for prompt intervention. In this study, we systematically reviewed morphologic, immunophenotypic, and cytogenetic findings in paired diagnostic and relapsed APL cases and describe and quantify the changes in blast morphology at relapse. Methods: By electronic database search, we identified eight paired diagnostic and relapsed APL cases for which peripheral blood or bone marrow smears were available for review. For two cases, diagnostic material was available for relapse after hematopoietic cell transplantation. Results: Neoplastic hypergranular or microgranular promyelocytes with indented or bivalve nuclei predominated at diagnosis in all patients. Most patients had undifferentiated blasts at relapse and/or hypergranular blast equivalents with round to oval nuclei. Classic acute promyelocytic leukemia cells with bivalve nuclei and bundles of cytoplasmic Auer rods were easily identifiable in fewer than half of cases at diagnosis and rare to absent in all relapsed cases. Conclusions: Morphologic features of relapsed APL overlap with other types of acute myeloid leukemia, creating diagnostic challenges, especially if no history is available when relapsing patients seek treatment for care.
[Mh] Termos MeSH primário: Células Precursoras de Granulócitos/patologia
Leucemia Promielocítica Aguda/diagnóstico
Leucemia Promielocítica Aguda/patologia
Recidiva Local de Neoplasia/diagnóstico
Recidiva Local de Neoplasia/patologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Diagnóstico Diferencial
Feminino
Seres Humanos
Imunofenotipagem
Leucemia Mieloide Aguda/diagnóstico
Masculino
Meia-Idade
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170414
[Lr] Data última revisão:
170414
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170122
[St] Status:MEDLINE
[do] DOI:10.1093/ajcp/aqw202


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[PMID]:28093826
[Au] Autor:Miyamoto K; Sumida M; Yuasa-Sunagawa M; Saito K
[Ad] Endereço:Department of Pharmaceutical Health Care, Faculty of Pharmaceutical Sciences, Himeji Dokkyo University, Hyogo, Japan.
[Ti] Título:Highly sensitive detection of E2 activity in ubiquitination using an artificial RING finger.
[So] Source:J Pept Sci;23(3):222-227, 2017 Mar.
[Is] ISSN:1099-1387
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The ubiquitin-conjugating (E2) enzymes of protein ubiquitination are associated with various diseases such as leukemia, lung cancer, and breast cancer. Rapid and accurate detection of E2 enzymatic activities remains poor. Here, we described the detection of E2 activity on a signal accumulation ISFET biosensor (AMIS sensor) using an artificial RING finger (ARF). The use of ARF enables the simplified detection of E2 activity without a substrate. The high-sensitivity quantitative detection of E2 activities was demonstrated via real-time monitoring over a response range of femtomolar to micromolar concentrations. Furthermore, the monitoring of E2 activities was successfully achieved using human acute promyelocytic leukemia cells following treatment with the anticancer drug bortezomib, which allowed the assessment of the pathological conditions. This strategy is extremely simple and convenient, and the present detection could be widely applied to specific E2s for various types of cancers. Copyright © 2017 European Peptide Society and John Wiley & Sons, Ltd.
[Mh] Termos MeSH primário: Técnicas Biossensoriais/métodos
Regulação Leucêmica da Expressão Gênica
Células Precursoras de Granulócitos/metabolismo
Peptidomiméticos/metabolismo
Prótons
Enzimas de Conjugação de Ubiquitina/análise
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Antineoplásicos/farmacologia
Técnicas Biossensoriais/instrumentação
Bortezomib/farmacologia
Linhagem Celular Tumoral
Técnicas Eletroquímicas
Células Precursoras de Granulócitos/efeitos dos fármacos
Células Precursoras de Granulócitos/patologia
Seres Humanos
Peptidomiméticos/síntese química
Domínios RING Finger/genética
Transdução de Sinais
Técnicas de Síntese em Fase Sólida
Ubiquitina/genética
Ubiquitina/metabolismo
Enzimas de Conjugação de Ubiquitina/genética
Enzimas de Conjugação de Ubiquitina/metabolismo
Ubiquitinação/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Peptidomimetics); 0 (Protons); 0 (Ubiquitin); 69G8BD63PP (Bortezomib); EC 2.3.2.23 (Ubiquitin-Conjugating Enzymes)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170307
[Lr] Data última revisão:
170307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170118
[St] Status:MEDLINE
[do] DOI:10.1002/psc.2972


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[PMID]:28053193
[Au] Autor:Cao M; Li T; He Z; Wang L; Yang X; Kou Y; Zou L; Dong X; Novakovic VA; Bi Y; Kou J; Yu B; Fang S; Wang J; Zhou J; Shi J
[Ad] Endereço:Department of Hematology, The First Hospital, Harbin Medical University, Harbin, China.
[Ti] Título:Promyelocytic extracellular chromatin exacerbates coagulation and fibrinolysis in acute promyelocytic leukemia.
[So] Source:Blood;129(13):1855-1864, 2017 Mar 30.
[Is] ISSN:1528-0020
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Despite routine treatment of unselected acute promyelocytic leukemia (APL) with all- -retinoic acid (ATRA), early death because of hemorrhage remains unacceptably common, and the mechanism underlying this complication remains elusive. We have recently demonstrated that APL cells undergo a novel cell death program, termed ETosis, which involves release of extracellular chromatin. However, the role of promyelocytic extracellular chromatin in APL-associated coagulation remains unclear. Our objectives were to identify the novel role of ATRA-promoted extracellular chromatin in inducing a hypercoagulable and hyperfibrinolytic state in APL and to evaluate its interaction with fibrin and endothelial cells (ECs). Results from a series of coagulation assays have shown that promyelocytic extracellular chromatin increases thrombin and plasmin generation, causes a shortening of plasma clotting time of APL cells, and increases fibrin formation. DNase I but not anti-tissue factor antibody could inhibit these effects. Immunofluorescence staining showed that promyelocytic extracellular chromatin and phosphatidylserine on APL cells provide platforms for fibrin deposition and render clots more resistant to fibrinolysis. Additionally, coincubation assays revealed that promyelocytic extracellular chromatin is cytotoxic to ECs, converting them to a procoagulant phenotype. This cytotoxity was blocked by DNase I by 20% or activated protein C by 31%. Our current results thus delineate the pathogenic role of promyelocytic extracellular chromatin in APL coagulopathy. Furthermore, the remaining coagulation disturbance in high-risk APL patients after ATRA administration may be treatable by intrinsic pathway inhibition via accelerating extracellular chromatin degradation.
[Mh] Termos MeSH primário: Coagulação Sanguínea
Cromatina/patologia
Cromatina/fisiologia
Fibrinólise
Leucemia Promielocítica Aguda/complicações
[Mh] Termos MeSH secundário: Células Cultivadas
Cromatina/ultraestrutura
Células Endoteliais
Fibrina/metabolismo
Células Precursoras de Granulócitos/patologia
Seres Humanos
Leucemia Promielocítica Aguda/sangue
Tretinoína/farmacologia
Células Tumorais Cultivadas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chromatin); 5688UTC01R (Tretinoin); 9001-31-4 (Fibrin)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170823
[Lr] Data última revisão:
170823
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170106
[St] Status:MEDLINE
[do] DOI:10.1182/blood-2016-09-739334


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[PMID]:27833171
[Au] Autor:Kupsa T; Vanek J; Pavel Z; Jebavy L; Horacek JM
[Ad] Endereço:Department of Military Internal Medicine and Military Hygiene, University of Defence, Faculty of Military Health Sciences (FMHS), Hradec Kralove, Czech Republic.
[Ti] Título:Serum levels of soluble adhesion molecules in newly diagnosed acute myeloid leukemia and in complete remission suggest endothelial cell activation by myeloblasts.
[So] Source:Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub;161(1):92-99, 2017 Mar.
[Is] ISSN:1213-8118
[Cp] País de publicação:Czech Republic
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AND AIMS: Despite high-dose multi-agent chemotherapy and allogeneic stem cell transplantation, the relapse rate of acute myeloid leukemia (AML) is high. Further, the disease is highly resistent to drugs. We speculated that deeper understanding of AML-endothelial cell interactions might provide new targets for selective modulation of the AML microenvironment and form the basis for novel treatment approaches. In this study, we evaluated levels of endothelium derived soluble adhesion molecules in active disease and in complete remission (CR) and their relationship with inflammatory cytokines. METHODS: Baseline serum levels of 25 cytokines and 5 soluble adhesion molecules were measured in 84 AML patients using biochip array technology. CR samples were evaluated in 44 patients of this cohort. The control group consisted of 15 healthy blood donors. RESULTS: All analytes were independent of age or disease origin. Some correlations were restricted to active AML, some were ubiquitous and some were found in remission. In active disease, E-selectin (E-SEL) and VCAM-1 correlated with leukocyte count, E-SEL correlated with P-selectin (P-SEL). Platelet count related to IL-7, EGF and VEGF but not to P-SEL. In CR, P-SEL correlated with platelet count and EGF but not with E-SEL. There was no relationship of P-SEL and E-SEL in the control group. CONCLUSIONS: Leukemic activity is associated with a different pattern of soluble adhesion molecule levels. Both E-SEL and P-SEL may be derived from endothelial cells. Their levels correlated in active disease. E-SEL correlated with leukocyte count. In CR, P-SEL physiologically correlated with platelet count. The correlation with E-SEL was insignificant and absent in the control group. Our data suggest activation of endothelial cells in the presence of myeloblasts.
[Mh] Termos MeSH primário: Moléculas de Adesão Celular/metabolismo
Células Endoteliais/fisiologia
Células Precursoras de Granulócitos/fisiologia
Leucemia Mieloide Aguda/sangue
[Mh] Termos MeSH secundário: Proteína C-Reativa/metabolismo
Estudos de Casos e Controles
Citocinas/metabolismo
Feminino
Seres Humanos
Contagem de Leucócitos
Masculino
Meia-Idade
Indução de Remissão
Fator A de Crescimento do Endotélio Vascular/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cell Adhesion Molecules); 0 (Cytokines); 0 (Vascular Endothelial Growth Factor A); 9007-41-4 (C-Reactive Protein)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161112
[St] Status:MEDLINE
[do] DOI:10.5507/bp.2016.054



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