Base de dados : MEDLINE
Pesquisa : A11.251.210.100.775 [Categoria DeCS]
Referências encontradas : 246 [refinar]
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[PMID]:28166708
[Au] Autor:Benevides Bahiense J; Marques FM; Figueira MM; Vargas TS; Kondratyuk TP; Endringer DC; Scherer R; Fronza M
[Ad] Endereço:a Programa de Pós-Graduação em Ciências Farmacêuticas , Universidade Vila Velha , Vila Velha , Espirito Santo , Brazil.
[Ti] Título:Potential anti-inflammatory, antioxidant and antimicrobial activities of Sambucus australis.
[So] Source:Pharm Biol;55(1):991-997, 2017 Dec.
[Is] ISSN:1744-5116
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CONTEXT: Sambucus australis Cham. & Schltdl. (Adoxaceae) is used in Brazilian folk medicine to treat inflammatory disorders. OBJECTIVE: To evaluate the in vitro anti-inflammatory, antioxidant and antimicrobial properties of S. australis. MATERIALS AND METHODS: The anti-inflammatory activity of ethanol extracts of the leaf and bark of S. australis (1-100 µg/mL) were studied in lipopolysaccharide/interferon γ stimulated murine macrophages RAW 264.7 cells (24 h incubation) by investigating the release of nitric oxide (NO) and tumour necrosis factor-alpha (TNF-α) and in the TNF-α-induced nuclear factor kappa (NF-κB) assay. Minimum inhibitory concentration (MIC) was determined by the microdilution test (24 h incubation). Antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP) and the NO scavenging assays. Chemical composition was assessed by LC-MS/MS. RESULTS: Antioxidant activities in the DPPH (IC 43.5 and 66.2 µg/mL), FRAP (IC 312.6 and 568.3 µg/mL) and NO radical scavenging assays (IC 285.0 and 972.6 µg/mL) were observed in the leaf and bark ethanol extracts, respectively. Solely the leaf extract showed significant inhibition of NO and TNF-α production in RAW264.7 cells at concentrations of 2 and 100 µg/mL, respectively, and suppression of TNF-α inhibition of NF-κB by 12.8 and 20.4% at concentrations of 50 and 100 µg/mL, respectively. The extract also exhibited antibacterial activity against Salmonella typhimurium (MIC 250 µg/mL) and Klebsiella pneumoniae (MIC 250 µg/mL). LC-MS/MS revealed the presence of chlorogenic acid and rutin as major compounds. DISCUSSION AND CONCLUSION: The results indicate that the ethanol leaf extract of S. australis exhibit prominent anti-inflammatory effects.
[Mh] Termos MeSH primário: Anti-Infecciosos/farmacologia
Anti-Inflamatórios/farmacologia
Antioxidantes/farmacologia
Klebsiella pneumoniae/efeitos dos fármacos
Macrófagos/efeitos dos fármacos
Extratos Vegetais/farmacologia
Salmonella typhimurium/efeitos dos fármacos
Sambucus/química
[Mh] Termos MeSH secundário: Animais
Anti-Infecciosos/química
Anti-Infecciosos/isolamento & purificação
Anti-Inflamatórios/química
Anti-Inflamatórios/isolamento & purificação
Antioxidantes/química
Antioxidantes/isolamento & purificação
Compostos de Bifenilo/química
Cloretos/química
Ácido Clorogênico/isolamento & purificação
Ácido Clorogênico/farmacologia
Relação Dose-Resposta a Droga
Etanol/química
Compostos Férricos/química
Células HEK293
Seres Humanos
Mediadores da Inflamação/metabolismo
Klebsiella pneumoniae/crescimento & desenvolvimento
Lipopolissacarídeos/farmacologia
Macrófagos/metabolismo
Camundongos
Testes de Sensibilidade Microbiana
NF-kappa B/genética
NF-kappa B/metabolismo
Óxido Nítrico/metabolismo
Oxirredução
Fitoterapia
Picratos/química
Casca de Planta
Extratos Vegetais/química
Extratos Vegetais/isolamento & purificação
Folhas de Planta
Plantas Medicinais
Células RAW 264.7
Rutina/isolamento & purificação
Rutina/farmacologia
Salmonella typhimurium/crescimento & desenvolvimento
Solventes/química
Células Swiss 3T3
Transfecção
Fator de Necrose Tumoral alfa/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Anti-Inflammatory Agents); 0 (Antioxidants); 0 (Biphenyl Compounds); 0 (Chlorides); 0 (Ferric Compounds); 0 (Inflammation Mediators); 0 (Lipopolysaccharides); 0 (NF-kappa B); 0 (Picrates); 0 (Plant Extracts); 0 (Solvents); 0 (Tumor Necrosis Factor-alpha); 318ADP12RI (Chlorogenic Acid); 31C4KY9ESH (Nitric Oxide); 3K9958V90M (Ethanol); 5G06TVY3R7 (Rutin); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl); U38V3ZVV3V (ferric chloride)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170309
[Lr] Data última revisão:
170309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170208
[St] Status:MEDLINE
[do] DOI:10.1080/13880209.2017.1285324


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[PMID]:28024998
[Au] Autor:Yu X; Pegram CN; Bigner DD; Chandramohan V
[Ad] Endereço:Department of Pathology, Duke University Medical Center, Durham, NC, USA. Electronic address: xin.yu@duke.edu.
[Ti] Título:Development and validation of a cell-based fluorescent method for measuring antibody affinity.
[So] Source:J Immunol Methods;442:49-53, 2017 Mar.
[Is] ISSN:1872-7905
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Monoclonal antibodies have become essential tools for diagnostic and therapeutic purposes. Antibody affinity is one of the critical factors influencing the therapeutic success of tumor-targeting antibodies. Therefore, developing an accurate and reliable method for determining antibody affinity is crucial. In this study, we describe a fluorescent cell-based immunosorbent assay that can accurately measure antibody affinity (K ) in the nanomolar range. This method involves the addition of fluorescently labeled antibodies to antigen-positive and antigen-negative cell lines fixed on 96-well plates. The fluorescent signals from nonspecific binding to negative control cell lines is subtracted from the specific binding to the antigen-positive cell lines. The K values obtained by this method were comparable with values obtained by the flow cytometry and radioactive (I ) scatchard assay. Our results demonstrate that this modified cell-based fluorescent method allows for a convenient and efficient identification of therapeutically relevant leads.
[Mh] Termos MeSH primário: Anticorpos Monoclonais/imunologia
Afinidade de Anticorpos
Antígenos/imunologia
Bioensaio/métodos
Espectrometria de Fluorescência
[Mh] Termos MeSH secundário: Animais
Anticorpos Monoclonais/metabolismo
Antígenos/genética
Antígenos/metabolismo
Sítios de Ligação de Anticorpos
Ligação Competitiva
Linhagem Celular Tumoral
Proteoglicanas de Sulfatos de Condroitina/imunologia
Proteoglicanas de Sulfatos de Condroitina/metabolismo
Citometria de Fluxo
Células HEK293
Seres Humanos
Glicoproteínas de Membrana/imunologia
Glicoproteínas de Membrana/metabolismo
Proteínas de Membrana/imunologia
Proteínas de Membrana/metabolismo
Camundongos
Mutação
Ligação Proteica
Receptor do Fator de Crescimento Epidérmico/genética
Receptor do Fator de Crescimento Epidérmico/imunologia
Receptor do Fator de Crescimento Epidérmico/metabolismo
Reprodutibilidade dos Testes
Células Swiss 3T3
Transfecção
Células Tumorais Cultivadas
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Antigens); 0 (CSPG4 protein, human); 0 (Chondroitin Sulfate Proteoglycans); 0 (Membrane Glycoproteins); 0 (Membrane Proteins); 0 (PDPN protein, human); EC 2.7.10.1 (EGFR protein, human); EC 2.7.10.1 (Receptor, Epidermal Growth Factor)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161228
[St] Status:MEDLINE


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[PMID]:27927082
[Au] Autor:Selestino Neta MC; Vittorazzi C; Guimarães AC; Martins JD; Fronza M; Endringer DC; Scherer R
[Ad] Endereço:a Department of Pharmacy , Post-Graduated Program of Pharmaceutical Sciences University Vila Velha , Espírito Santo , Brazil.
[Ti] Título:Effects of ß-caryophyllene and Murraya paniculata essential oil in the murine hepatoma cells and in the bacteria and fungi 24-h time-kill curve studies.
[So] Source:Pharm Biol;55(1):190-197, 2017 Dec.
[Is] ISSN:1744-5116
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CONTEXT: Orange Jessamine [Murraya paniculata L. (Rutaceae)] has been used worldwide in folk medicine as an anti-inflammatory, antibiotic and analgesic. OBJECTIVE: The objective of this study is to investigate the in vitro antioxidant, cytotoxic, antibacterial and antifungal activity and the time-kill curve studies of orange jessamine essential oil and ß-caryophyllene, as well as the chemical composition of the essential oil. MATERIAL AND METHODS: The cytotoxic activity of M. paniculata and ß-caryophyllene (7.8-500 µg/mL) was evaluated using the MTT assay on normal fibroblasts and hepatoma cells. The minimal inhibitory concentration and time-kill curves (24 h) were evaluated against those of Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, Enterococcus faecallis, Aspergillus (niger, fumigates and parasiticum) and F. solani by the broth microdilution method. The antioxidant activity was measured by the DPPH and ABTS assays. Chemical composition was evaluated by GC/MS analyses. RESULTS: GC/MS analyses identified 13 compounds, with ß-caryophyllene as the major compound. The oil exhibited moderate antibacterial activity (MIC <1.0 mg/mL) and strong antifungal activity. Time-kill curve studies showed that either the essential oil or ß-caryophyllene presented rapid bacterial killing (4 h for S. aureus) and fungicidal effect (2-4 h for F. solani); however, both displayed weak free radical scavenger capacity. The cytotoxic activity exhibited a prominent selective effect against hepatoma cancer cells (IC value =63.7 µg/mL) compared with normal fibroblasts (IC value =195.0 µg/mL), whereas the ß-caryophyllene showed low cytotoxicity. DISCUSSION AND CONCLUSION: The experimental data suggest that the activities of M. paniculata essential oil are due to the synergistic action among its components.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Antifúngicos/farmacologia
Antineoplásicos Fitogênicos/farmacologia
Carcinoma Hepatocelular/tratamento farmacológico
Fusarium/efeitos dos fármacos
Neoplasias Hepáticas/tratamento farmacológico
Murraya/química
Óleos Voláteis/farmacologia
Extratos Vegetais/farmacologia
Óleos Vegetais/farmacologia
Sesquiterpenos/farmacologia
Staphylococcus aureus/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Antibacterianos/isolamento & purificação
Antifúngicos/isolamento & purificação
Antineoplásicos Fitogênicos/isolamento & purificação
Antioxidantes/isolamento & purificação
Antioxidantes/farmacologia
Carcinoma Hepatocelular/patologia
Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
Relação Dose-Resposta a Droga
Fibroblastos/efeitos dos fármacos
Fibroblastos/patologia
Fusarium/crescimento & desenvolvimento
Cromatografia Gasosa-Espectrometria de Massas
Concentração Inibidora 50
Neoplasias Hepáticas/patologia
Camundongos
Testes de Sensibilidade Microbiana
Óleos Voláteis/isolamento & purificação
Fitoterapia
Extratos Vegetais/isolamento & purificação
Folhas de Planta
Óleos Vegetais/isolamento & purificação
Plantas Medicinais
Sesquiterpenos/isolamento & purificação
Staphylococcus aureus/crescimento & desenvolvimento
Células Swiss 3T3
Fatores de Tempo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Antifungal Agents); 0 (Antineoplastic Agents, Phytogenic); 0 (Antioxidants); 0 (Oils, Volatile); 0 (Plant Extracts); 0 (Plant Oils); 0 (Sesquiterpenes); BHW853AU9H (caryophyllene)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170310
[Lr] Data última revisão:
170310
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161209
[St] Status:MEDLINE


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[PMID]:27083794
[Au] Autor:Foglarová M; Chmelar J; Huerta-Angeles G; Vágnerová H; Kulhánek J; Barton Tománková K; Minarík A; Velebný V
[Ad] Endereço:Contipro Biotech s.r.o., Dolní Dobrouc 401, Dolní Dobrouc 561 02, Czech Republic. Electronic address: Marcela.Foglarova@contipro.com.
[Ti] Título:Water-insoluble thin films from palmitoyl hyaluronan with tunable properties.
[So] Source:Carbohydr Polym;144:68-75, 2016 Jun 25.
[Is] ISSN:1879-1344
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Hyaluronan (HA) films exhibit properties suitable for various biomedical applications, but the solubility of HA limits their use in aqueous environments. Therefore, we developed water insoluble films based on palmitoyl esters of HA (pHA). Films were prepared from pHA samples with various degrees of substitution (DS) and molecular weights and their mechanical properties and swelling were characterized. Additionally, scanning electron microscopy and atomic force microscopy were used for visualization. Despite being prepared by solution casting, the films had a very smooth surface and were homogeneous in thickness. The film properties were in accordance with the polymer DS and molecular weight, enabling to tailor them for future applications by choosing a suitable pHA material. The behavior of the films toward cells was assessed in vitro. All films were non-cytotoxic and showed no adhesion of cells. These results show that the developed films are suitable candidates for various biomedical applications such as tissue engineering or wound healing.
[Mh] Termos MeSH primário: Ácido Hialurônico/química
Palmitatos/química
[Mh] Termos MeSH secundário: Acilação
Animais
Adesão Celular/efeitos dos fármacos
Linhagem Celular Tumoral
Citometria de Fluxo
Seres Humanos
Ácido Hialurônico/análogos & derivados
Ácido Hialurônico/toxicidade
Camundongos
Microscopia de Força Atômica
Microscopia Eletrônica de Varredura
Palmitatos/toxicidade
Solubilidade
Células Swiss 3T3
Resistência à Tração
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Palmitates); 059QF0KO0R (Water); 9004-61-9 (Hyaluronic Acid)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170623
[Lr] Data última revisão:
170623
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160417
[St] Status:MEDLINE


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[PMID]:26880200
[Au] Autor:Inaba H; Goto H; Kasahara K; Kumamoto K; Yonemura S; Inoko A; Yamano S; Wanibuchi H; He D; Goshima N; Kiyono T; Hirotsune S; Inagaki M
[Ad] Endereço:Division of Biochemistry, Aichi Cancer Center Research Institute, Nagoya 464-8681, Japan.
[Ti] Título:Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway.
[So] Source:J Cell Biol;212(4):409-23, 2016 Feb 15.
[Is] ISSN:1540-8140
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Primary cilia protrude from the surface of quiescent cells and disassemble at cell cycle reentry. We previously showed that ciliary reassembly is suppressed by trichoplein-mediated Aurora A activation pathway in growing cells. Here, we report that Ndel1, a well-known modulator of dynein activity, localizes at the subdistal appendage of the mother centriole, which nucleates a primary cilium. In the presence of serum, Ndel1 depletion reduces trichoplein at the mother centriole and induces unscheduled primary cilia formation, which is reverted by forced trichoplein expression or coknockdown of KCTD17 (an E3 ligase component protein for trichoplein). Serum starvation induced transient Ndel1 degradation, subsequent to the disappearance of trichoplein at the mother centriole. Forced expression of Ndel1 suppressed trichoplein degradation and axonemal microtubule extension during ciliogenesis, similar to trichoplein induction or KCTD17 knockdown. Most importantly, the proportion of ciliated and quiescent cells was increased in the kidney tubular epithelia of newborn Ndel1-hypomorphic mice. Thus, Ndel1 acts as a novel upstream regulator of the trichoplein-Aurora A pathway to inhibit primary cilia assembly.
[Mh] Termos MeSH primário: Aurora Quinase A/metabolismo
Proteínas de Transporte/metabolismo
Proliferação Celular
Células Epiteliais/enzimologia
Transdução de Sinais
[Mh] Termos MeSH secundário: Proteínas Adaptadoras de Transdução de Sinal/genética
Proteínas Adaptadoras de Transdução de Sinal/metabolismo
Animais
Animais Recém-Nascidos
Aurora Quinase A/genética
Proteínas de Transporte/genética
Pontos de Checagem do Ciclo Celular
Centríolos/enzimologia
Cílios/enzimologia
Genótipo
Células HeLa
Seres Humanos
Túbulos Renais/citologia
Túbulos Renais/enzimologia
Camundongos
Camundongos Knockout
Microtúbulos/enzimologia
Fenótipo
Estabilidade Proteica
Proteólise
Interferência de RNA
Células Swiss 3T3
Fatores de Tempo
Transfecção
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Adaptor Proteins, Signal Transducing); 0 (Carrier Proteins); 0 (KCTD17 protein, human); 0 (NDEL1 protein, human); 0 (Ndel1 protein, mouse); 0 (TCHP protein, human); EC 2.7.11.1 (AURKA protein, human); EC 2.7.11.1 (Aurka protein, mouse); EC 2.7.11.1 (Aurora Kinase A)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:160815
[Lr] Data última revisão:
160815
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160217
[St] Status:MEDLINE
[do] DOI:10.1083/jcb.201507046


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[PMID]:26545237
[Au] Autor:Brown TA; Tkachuk AN; Clayton DA
[Ad] Endereço:Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, United States of America.
[Ti] Título:Mitochondrial Transcription Factor A (TFAM) Binds to RNA Containing 4-Way Junctions and Mitochondrial tRNA.
[So] Source:PLoS One;10(11):e0142436, 2015.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mitochondrial DNA (mtDNA) is maintained within nucleoprotein complexes known as nucleoids. These structures are highly condensed by the DNA packaging protein, mitochondrial Transcription Factor A (TFAM). Nucleoids also include RNA, RNA:DNA hybrids, and are associated with proteins involved with RNA processing and mitochondrial ribosome biogenesis. Here we characterize the ability of TFAM to bind various RNA containing substrates in order to determine their role in TFAM distribution and function within the nucleoid. We find that TFAM binds to RNA-containing 4-way junctions but does not bind appreciably to RNA hairpins, internal loops, or linear RNA:DNA hybrids. Therefore the RNA within nucleoids largely excludes TFAM, and its distribution is not grossly altered with removal of RNA. Within the cell, TFAM binds to mitochondrial tRNAs, consistent with our RNA 4-way junction data. Kinetic binding assays and RNase-insensitive TFAM distribution indicate that DNA remains the preferred substrate within the nucleoid. However, TFAM binds to tRNA with nanomolar affinity and these complexes are not rare. TFAM-immunoprecipitated tRNAs have processed ends, suggesting that binding is not specific to RNA precursors. The amount of each immunoprecipitated tRNA is not well correlated with tRNA celluar abundance, indicating unequal TFAM binding preferences. TFAM-mt-tRNA interaction suggests potentially new functions for this protein.
[Mh] Termos MeSH primário: Proteínas de Ligação a DNA/metabolismo
Proteínas de Grupo de Alta Mobilidade/metabolismo
RNA/química
RNA/metabolismo
[Mh] Termos MeSH secundário: Animais
Cinética
Camundongos
Conformação de Ácido Nucleico
Ligação Proteica
RNA de Transferência/química
RNA de Transferência/metabolismo
Ressonância de Plasmônio de Superfície
Células Swiss 3T3
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA-Binding Proteins); 0 (High Mobility Group Proteins); 0 (RNA, mitochondrial); 0 (Tfam protein, mouse); 63231-63-0 (RNA); 9014-25-9 (RNA, Transfer)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151107
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0142436


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[PMID]:26392024
[Au] Autor:Chugh RM; Chaturvedi M; Yerneni LK
[Ad] Endereço:Cell Biology Laboratory, National Institute of Pathology (ICMR), New Delhi, India.
[Ti] Título:An evaluation of the choice of feeder cell growth arrest for the production of cultured epidermis.
[So] Source:Burns;41(8):1788-1795, 2015 Dec.
[Is] ISSN:1879-1409
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Growth arrested 3T3 cells have been used as feeder cells in human epidermal keratinocyte cultures to produce cultured epidermal autografts for the treatment of burns. The feeder cells were ideally growth-arrested by gamma-irradiation. Alternatively, growth arrest by mitomycin C treatment is a cost effective option. We compared the functional efficacy of these two approaches in keratinocyte cultures by colony forming efficiency, the net growth area of colonies, BrdU labeling and histological features of cultured epidermal sheets. The growth area estimation involved a semi-automated digital technique using the Adobe Photoshop and comprised of isolation and enumeration of red pixels in Rhodamine B-stained keratinocyte colonies. A further refinement of the technique led to the identification of critical steps to increasing the degree of accuracy and enabling its application as an extension of colony formation assay. The results on feeder cell functionality revealed that the gamma irradiated feeders influenced significantly higher colony forming efficiency and larger growth area than the mitomycin C treated feeders. The BrdU labeling study indicated significant stimulation of the overall keratinocyte proliferation by the gamma irradiated feeders. The cultured epidermal sheets produced by gamma feeders were relatively thicker than those produced by mitomycin C feeders. We discussed the clinical utility of mitomycin C feeders from the viewpoint of cost-effective burn care in developing countries.
[Mh] Termos MeSH primário: Alquilantes/farmacologia
Proliferação Celular
Técnicas de Cocultura/métodos
Células Alimentadoras/efeitos dos fármacos
Raios gama
Queratinócitos/citologia
Mitomicina/farmacologia
[Mh] Termos MeSH secundário: Animais
Ensaio de Unidades Formadoras de Colônias
Epiderme/citologia
Epiderme/crescimento & desenvolvimento
Células Alimentadoras/efeitos da radiação
Seres Humanos
Camundongos
Células Swiss 3T3
Engenharia Tecidual
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Alkylating Agents); 50SG953SK6 (Mitomycin)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:170730
[Lr] Data última revisão:
170730
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150923
[St] Status:MEDLINE


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[PMID]:26077760
[Au] Autor:Crepin VF; Habibzay M; Glegola-Madejska I; Guenot M; Collins JW; Frankel G
[Ad] Endereço:MRC Centre for Molecular Bacteriology and Infection, Department of Life Sciences, Imperial College, London, United Kingdom v.crepin-sevenou@imperial.ac.uk g.frankel@imperial.ac.uk.
[Ti] Título:Tir Triggers Expression of CXCL1 in Enterocytes and Neutrophil Recruitment during Citrobacter rodentium Infection.
[So] Source:Infect Immun;83(9):3342-54, 2015 Sep.
[Is] ISSN:1098-5522
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The hallmarks of enteropathogenic Escherichia coli (EPEC) infection are formation of attaching and effacing (A/E) lesions on mucosal surfaces and actin-rich pedestals on cultured cells, both of which are dependent on the type III secretion system effector Tir. Following translocation into cultured cells and clustering by intimin, Tir Y474 is phosphorylated, leading to recruitment of Nck, activation of N-WASP, and actin polymerization via the Arp2/3 complex. A secondary, weak, actin polymerization pathway is triggered via an NPY motif (Y454). Importantly, Y454 and Y474 play no role in A/E lesion formation on mucosal surfaces following infection with the EPEC-like mouse pathogen Citrobacter rodentium. In this study, we investigated the roles of Tir segments located upstream of Y451 and downstream of Y471 in C. rodentium colonization and A/E lesion formation. We also tested the role that Tir residues Y451 and Y471 play in host immune responses to C. rodentium infection. We found that deletion of amino acids 382 to 462 or 478 to 547 had no impact on the ability of Tir to mediate A/E lesion formation, although deletion of amino acids 478 to 547 affected Tir translocation. Examination of enterocytes isolated from infected mice revealed that a C. rodentium strain expressing Tir_Y451A/Y471A recruited significantly fewer neutrophils to the colon and triggered less colonic hyperplasia on day 14 postinfection than the wild-type strain. Consistently, enterocytes isolated from mice infected with C. rodentium expressing Tir_Y451A/Y471A expressed significantly less CXCL1. These result show that Tir-induced actin remodeling plays a direct role in modulation of immune responses to C. rodentium infection.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Sistemas de Secreção Bacterianos/fisiologia
Quimiocina CXCL1/biossíntese
Citrobacter rodentium
Infecções por Enterobacteriaceae/imunologia
Enterócitos/metabolismo
Infiltração de Neutrófilos/fisiologia
[Mh] Termos MeSH secundário: Actinas/metabolismo
Animais
Proteínas de Bactérias/genética
Infecções por Enterobacteriaceae/metabolismo
Feminino
Citometria de Fluxo
Camundongos
Camundongos Endogâmicos C57BL
Mutagênese Sítio-Dirigida
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Células Swiss 3T3
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Actins); 0 (Bacterial Proteins); 0 (Bacterial Secretion Systems); 0 (Chemokine CXCL1); 0 (Cxcl1 protein, mouse)
[Em] Mês de entrada:1511
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150617
[St] Status:MEDLINE
[do] DOI:10.1128/IAI.00291-15


  9 / 246 MEDLINE  
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[PMID]:26041886
[Au] Autor:Peng B; Ganapathy S; Shen L; Huang J; Yi B; Zhou X; Dai W; Chen C
[Ad] Endereço:Center for Drug Discovery, Northeastern University, Boston, MA, USA.
[Ti] Título:Targeting Bcl-2 stability to sensitize cells harboring oncogenic ras.
[So] Source:Oncotarget;6(26):22328-37, 2015 Sep 08.
[Is] ISSN:1949-2553
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The pro-survival factor Bcl-2 and its family members are critical determinants of the threshold of the susceptibility of cells to apoptosis. Studies are shown that cells harboring an oncogenic ras were extremely sensitive to the inhibition of protein kinase C (PKC) and Bcl-2 could antagonize this apoptotic process. However, it remains unrevealed how Bcl-2 is being regulated in this apoptotic process. In this study, we investigate the role of Bcl-2 stability in sensitizing the cells harboring oncogenic K-ras to apoptosis triggered by PKC inhibitor GO6976. We demonstrated that Bcl-2 in Swiss3T3 cells ectopically expressing or murine lung cancer LKR cells harboring K-ras rapidly underwent ubiquitin-dependent proteasome pathway after the treatment of GO6976, accompanied with induction of apoptosis. In this process, Bcl-2 formed the complex with Keap-1 and Cul3. The mutation of serine-17 and deletion of BH-2 or 4 was required for Bcl-2 ubiquitination and degradation, which elevate the signal threshold for the induction of apoptosis in the cells following PKC inhibition. Thus, Bcl-2 appears an attractive target for the induction of apoptosis by PKC inhibition in cancer cells expressing oncogenic K-ras.
[Mh] Termos MeSH primário: Genes ras
Proteínas Proto-Oncogênicas c-bcl-2/genética
[Mh] Termos MeSH secundário: Animais
Apoptose/genética
Linhagem Celular Tumoral
Camundongos
Proteínas Proto-Oncogênicas c-bcl-2/metabolismo
Transdução de Sinais
Células Swiss 3T3
Transfecção
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Proto-Oncogene Proteins c-bcl-2)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:170503
[Lr] Data última revisão:
170503
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150605
[St] Status:MEDLINE


  10 / 246 MEDLINE  
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[PMID]:25937440
[Au] Autor:Redondo JA; Martínez-Campos E; Navarro R; Reinecke H; Elvira C; López-Lacomba JL; Gallardo A
[Ad] Endereço:Institute of Polymer Science and Technology, ICTP-CSIC, Juan de la Cierva 3, 28006 Madrid, Spain. Electronic address: jaredondo@ictp.csic.es.
[Ti] Título:Effect on in vitro cell response of the statistical insertion of N-(2-hydroxypropyl) methacrylamide on linear pro-dendronic polyamine's gene carriers.
[So] Source:Eur J Pharm Biopharm;93:303-10, 2015 Jun.
[Is] ISSN:1873-3441
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Statistical copolymers of N-(2-hydroxypropyl) methacrylamide (HPMA) and the dendronic methacrylic monomer 2-(3-(Bis(2-(diethylamino)ethyl)amino)propanamido)ethyl methacrylate (TEDETAMA, derived from N,N,N',N'-tetraethyldiethylenetriamine, TEDETA), were synthesized through radical copolymerization and evaluated in vitro as non-viral gene carriers. Three copolymers with nominal molar percentages of HPMA of 25%, 50% and 75% were prepared and studied comparatively to the positive controls poly-TEDETAMA and hyperbranched polyethyleneimine (PEI, 25kDa). Their ability to complex DNA at different N/P molar ratios, from 1/1 up to 8/1, was determined through agarose gel electrophoresis and Dynamic Light Scattering. The resulting complexes (polyplexes) were characterized and evaluated in vitro as possible non-viral gene carriers for Swiss-3T3 fibroblasts, using luciferase as reporter gene and a calcein cytocompatibility assay. All the copolymers, except the one with highest HPMA proportion (75 molar %) at the lowest N/P ratio, condensed DNA to a particle size between 100 and 300 nm. The copolymers with 25 and 50 molar % of HPMA displayed higher transfection efficiency and cytocompatibility than the positive controls poly-TEDETAMA and PEI. A higher proportion of HPMA (75 molar %) led to copolymers that displayed very low transfection efficiency, despite their full cytocompatibility even at the highest N/P ratio. These results indicate that the statistical combination of TEDETAMA and HPMA and its fine compositional tuning in the copolymers may fulfill the fine balance of transfection efficiency and cytocompatibility in a superior way to the control poly-TEDETAMA and PEI.
[Mh] Termos MeSH primário: Acrilamidas/síntese química
DNA/biossíntese
Dendrímeros/síntese química
Fibroblastos/metabolismo
Modelos Estatísticos
Polietilenoimina/síntese química
Transfecção/métodos
[Mh] Termos MeSH secundário: Acrilamidas/toxicidade
Animais
DNA/química
Dendrímeros/toxicidade
Eletroforese em Gel de Ágar
Fibroblastos/efeitos dos fármacos
Regulação da Expressão Gênica
Genes Reporter
Luz
Luciferases/biossíntese
Luciferases/genética
Camundongos
Estrutura Molecular
Conformação de Ácido Nucleico
Tamanho da Partícula
Polietilenoimina/análogos & derivados
Polietilenoimina/toxicidade
Espalhamento de Radiação
Células Swiss 3T3
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Acrylamides); 0 (Dendrimers); 9002-98-6 (Polyethyleneimine); 9007-49-2 (DNA); EC 1.13.12.- (Luciferases); R3F262Z4E0 (N-(2-hydroxypropyl)methacrylamide)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150505
[St] Status:MEDLINE



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