Base de dados : MEDLINE
Pesquisa : A11.251.800 [Categoria DeCS]
Referências encontradas : 3835 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 384 ir para página                         

  1 / 3835 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29184400
[Au] Autor:Millard M; Yakavets I; Zorin V; Kulmukhamedova A; Marchal S; Bezdetnaya L
[Ad] Endereço:Centre de Recherche en Automatique de Nancy, Centre National de la Recherche Scientifique UMR 7039, Université de Lorraine.
[Ti] Título:Drug delivery to solid tumors: the predictive value of the multicellular tumor spheroid model for nanomedicine screening.
[So] Source:Int J Nanomedicine;12:7993-8007, 2017.
[Is] ISSN:1178-2013
[Cp] País de publicação:New Zealand
[La] Idioma:eng
[Ab] Resumo:The increasing number of publications on the subject shows that nanomedicine is an attractive field for investigations aiming to considerably improve anticancer chemotherapy. Based on selective tumor targeting while sparing healthy tissue, carrier-mediated drug delivery has been expected to provide significant benefits to patients. However, despite reduced systemic toxicity, most nanodrugs approved for clinical use have been less effective than previously anticipated. The gap between experimental results and clinical outcomes demonstrates the necessity to perform comprehensive drug screening by using powerful preclinical models. In this context, in vitro three-dimensional models can provide key information on drug behavior inside the tumor tissue. The multicellular tumor spheroid (MCTS) model closely mimics a small avascular tumor with the presence of proliferative cells surrounding quiescent cells and a necrotic core. Oxygen, pH and nutrient gradients are similar to those of solid tumor. Furthermore, extracellular matrix (ECM) components and stromal cells can be embedded in the most sophisticated spheroid design. All these elements together with the physicochemical properties of nanoparticles (NPs) play a key role in drug transport, and therefore, the MCTS model is appropriate to assess the ability of NP to penetrate the tumor tissue. This review presents recent developments in MCTS models for a better comprehension of the interactions between NPs and tumor components that affect tumor drug delivery. MCTS is particularly suitable for the high-throughput screening of new nanodrugs.
[Mh] Termos MeSH primário: Sistemas de Liberação de Medicamentos/métodos
Ensaios de Seleção de Medicamentos Antitumorais/métodos
Nanomedicina/métodos
Neoplasias/tratamento farmacológico
Esferoides Celulares
[Mh] Termos MeSH secundário: Animais
Antineoplásicos/administração & dosagem
Antineoplásicos/farmacocinética
Portadores de Fármacos/administração & dosagem
Portadores de Fármacos/uso terapêutico
Matriz Extracelular/efeitos dos fármacos
Seres Humanos
Nanopartículas/administração & dosagem
Esferoides Celulares/química
Esferoides Celulares/efeitos dos fármacos
Esferoides Celulares/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Drug Carriers)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE
[do] DOI:10.2147/IJN.S146927


  2 / 3835 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28460441
[Au] Autor:Arora J; Sauer SJ; Tarpley M; Vermeulen P; Rypens C; Van Laere S; Williams KP; Devi GR; Dewhirst MW
[Ad] Endereço:Duke Cancer Institute, Duke University, Durham, NC, USA.
[Ti] Título:Inflammatory breast cancer tumor emboli express high levels of anti-apoptotic proteins: use of a quantitative high content and high-throughput 3D IBC spheroid assay to identify targeting strategies.
[So] Source:Oncotarget;8(16):25848-25863, 2017 Apr 18.
[Is] ISSN:1949-2553
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Inflammatory breast cancer (IBC) is one of the most lethal breast cancer variants; with existing therapy, 5-yr survival rate is only 35%. Current barriers to successful treatment of IBC include frequent infiltration and the presence of tumor cell clusters, termed tumor emboli, within the breast parenchyma and lymphatics. Prior studies have identified the role of anti-apoptotic signaling, in particular hyperactivation of NFκB and its target genes, in IBC pathobiology and therapeutic resistance. The objectives of this study were to: (1) determine if IBC tumor emboli express anti-apoptotic proteins and (2) develop a high content, multiparametric assay to assess the morphology of the IBC 3D spheroids and to optimize a high throughput format to screen for compounds that can inhibit the formation of the IBC tumor clusters/embolic structures. Immunohistochemical analysis of IBC patient tumor samples with documented tumor emboli revealed high NFκB (p65) staining along with expression of XIAP, a potent anti-apoptotic protein known to interact with NFκB signaling in enhancing survival of malignant cells. Subsequently, the high content assay developed allowed for simultaneous imaging and morphometric analysis, including count and viability of spheroids derived from SUM149, rSUM149 and SUM190 cells and its application to evaluate XIAP and NFκB inhibitory agents. We demonstrate the efficacy of the off-patent drug disulfiram when chelated with copper, which we had previously reported to inhibit NFκB signaling, was highly effective in disrupting both IBC spheroids and emboli grown in vitro. Taken together, these results identify a high-throughput approach to target tumor spheroid formation for drug discovery. Finally, disulfiram is a safe and approved drug for management of alcohol abuse, warranting its evaluation for repurposing in IBC therapy.
[Mh] Termos MeSH primário: Proteínas Reguladoras de Apoptose/genética
Neoplasias Inflamatórias Mamárias/genética
Neoplasias Inflamatórias Mamárias/patologia
Células Neoplásicas Circulantes/metabolismo
[Mh] Termos MeSH secundário: Proteínas Reguladoras de Apoptose/metabolismo
Biomarcadores Tumorais
Técnicas de Cultura de Células
Sobrevivência Celular/genética
Cobre/farmacologia
Dissulfiram/farmacologia
Feminino
Expressão Gênica
Ensaios de Triagem em Larga Escala
Seres Humanos
Neoplasias Inflamatórias Mamárias/metabolismo
Mitocôndrias/metabolismo
NF-kappa B/genética
NF-kappa B/metabolismo
Esferoides Celulares
Células Tumorais Cultivadas
Proteínas Inibidoras de Apoptose Ligadas ao Cromossomo X/genética
Proteínas Inibidoras de Apoptose Ligadas ao Cromossomo X/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Apoptosis Regulatory Proteins); 0 (Biomarkers, Tumor); 0 (NF-kappa B); 0 (X-Linked Inhibitor of Apoptosis Protein); 0 (XIAP protein, human); 789U1901C5 (Copper); TR3MLJ1UAI (Disulfiram)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.18632/oncotarget.15667


  3 / 3835 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28452807
[Au] Autor:Kim J; Kang SM; Lee HJ; Choi SY; Hong SH
[Ad] Endereço:Departments of aOral Microbiology and Immunology bOral and Maxillofacial Surgery, School of Dentistry, Kyungpook National University, Daegu, South Korea.
[Ti] Título:Oxytocin inhibits head and neck squamous cell carcinoma cell migration by early growth response-1 upregulation.
[So] Source:Anticancer Drugs;28(6):613-622, 2017 07.
[Is] ISSN:1473-5741
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The effect of oxytocin (OXT) on cancer invasion is controversial. Few studies have examined the effect of early growth response-1 (EGR1) on the invasion of head and neck squamous cell carcinoma (HNSCC). Here, we evaluated how EGR1 affects HNSCC cell migration through the molecular mechanism of OXT in exerting anti-invasion activity. Matrigel invasion and wound-healing assays were used to measure the in-vitro cell migration. The molecular mechanism of OXT was assessed by knockdown or overexpression of EGR1 in HNSCC cells. Three-dimensional (3-D) spheroids formation, followed by the image analysis for quantification was performed. OXT at 500 nmol/l increased mRNA and protein expression of E-cadherin without cytotoxicity. OXT upregulated mRNA and protein expression of EGR1 in 6 h. p53, phosphatase and tensin, and p21 expression was increased in an EGR1-dependent manner with OXT treatment. In addition, OXT significantly downregulated 3-D spheroids' formation according to spheroids' number and size. Our data showed that OXT downregulated HNSCC cell migration by EGR1 upregulation. OXT inhibited spheroids' formation of HNSCC cells under 3-D culture conditions.
[Mh] Termos MeSH primário: Carcinoma de Células Escamosas/tratamento farmacológico
Movimento Celular/efeitos dos fármacos
Proteína 1 de Resposta de Crescimento Precoce/biossíntese
Neoplasias de Cabeça e Pescoço/tratamento farmacológico
Ocitocina/farmacologia
[Mh] Termos MeSH secundário: Carcinoma de Células Escamosas/metabolismo
Carcinoma de Células Escamosas/patologia
Transição Epitelial-Mesenquimal/efeitos dos fármacos
MAP Quinases Reguladas por Sinal Extracelular/metabolismo
Neoplasias de Cabeça e Pescoço/metabolismo
Neoplasias de Cabeça e Pescoço/patologia
Seres Humanos
Invasividade Neoplásica
Metástase Neoplásica
Receptor do Fator de Crescimento Epidérmico/metabolismo
Esferoides Celulares
Células Tumorais Cultivadas
Regulação para Cima/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (EGR1 protein, human); 0 (Early Growth Response Protein 1); 50-56-6 (Oxytocin); EC 2.7.10.1 (EGFR protein, human); EC 2.7.10.1 (Receptor, Epidermal Growth Factor); EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1097/CAD.0000000000000501


  4 / 3835 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28460350
[Au] Autor:Jiang B; Yan L; Miao Z; Li E; Wong KH; Xu RH
[Ad] Endereço:Faculty of Health Sciences, University of Macau, Taipa, Macau.
[Ti] Título:Spheroidal formation preserves human stem cells for prolonged time under ambient conditions for facile storage and transportation.
[So] Source:Biomaterials;133:275-286, 2017 Jul.
[Is] ISSN:1878-5905
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Human stem cells are vulnerable to unfavorable conditions, and their transportation relies on costly and inconvenient cryopreservation. We report here that human mesenchymal stem cells (MSC) in spheroids survived ambient conditions (AC) many days longer than in monolayer. Under AC, the viability of MSC in spheroids remained >90% even after seven days, whereas MSC in monolayer mostly died fast. AC-exposed MSC spheroids, after recovery under normal monolayer culture conditions with controlled carbon dioxide and humidity contents, resumed typical morphology and proliferation, and retained differentiating and immunosuppressive capabilities. RNA-sequencing and other assays demonstrate that reduced cell metabolism and proliferation correlates to the enhanced survival of AC-exposed MSC in spheroids versus monolayer. Moreover, AC-exposed MSC, when injected as either single cells or spheroids, retained therapeutic effects in vivo in mouse colitis models. Spheroidal formation also prolonged survival and sustained pluripotency of human embryonic stem cells kept under AC. Therefore, this work offers an alternative and relatively simple method termed spheropreservation versus the conventional method cryopreservation. It shall remarkably simplify long-distance transportation of stem cells of these and probably also other types within temperature-mild areas, and facilitate therapeutic application of MSC as spheroids without further processing.
[Mh] Termos MeSH primário: Técnicas de Cultura de Células/métodos
Esferoides Celulares/citologia
Células-Tronco/citologia
[Mh] Termos MeSH secundário: Proliferação Celular/fisiologia
Sobrevivência Celular/fisiologia
Células Cultivadas
Seres Humanos
Células Mesenquimais Estromais/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


  5 / 3835 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28470516
[Au] Autor:Ivanov DP; Grabowska AM; Garnett MC
[Ad] Endereço:Cancer Biology, Division of Cancer and Stem Cells, School of Medicine, Queen's Medical Centre, University of Nottingham, Nottingham, NG7 2UH, UK. delyan.ivanov@nottingham.ac.uk.
[Ti] Título:High-Throughput Spheroid Screens Using Volume, Resazurin Reduction, and Acid Phosphatase Activity.
[So] Source:Methods Mol Biol;1601:43-59, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mainstream adoption of physiologically relevant three-dimensional models has been slow in the last 50 years due to long, manual protocols with poor reproducibility, high price, and closed commercial platforms. This chapter describes high-throughput, low-cost, open methods for spheroid viability assessment which use readily available reagents and open-source software to analyze spheroid volume, metabolism, and enzymatic activity. We provide two ImageJ macros for automated spheroid size determination-for both single images and images in stacks. We also share an Excel template spreadsheet allowing users to rapidly process spheroid size data, analyze plate uniformity (such as edge effects and systematic seeding errors), detect outliers, and calculate dose-response. The methods would be useful to researchers in preclinical and translational research planning to move away from simplistic monolayer studies and explore 3D spheroid screens for drug safety and efficacy without substantial investment in money or time.
[Mh] Termos MeSH primário: Sobrevivência Celular
Ensaios de Triagem em Larga Escala/métodos
Indicadores e Reagentes/metabolismo
Esferoides Celulares/fisiologia
[Mh] Termos MeSH secundário: Fosfatase Ácida/metabolismo
Encéfalo/citologia
Linhagem Celular Tumoral
Relação Dose-Resposta a Droga
Avaliação Pré-Clínica de Medicamentos
Ensaios de Triagem em Larga Escala/economia
Seres Humanos
Processamento de Imagem Assistida por Computador
Oxazinas/química
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Software
Esferoides Celulares/citologia
Esferoides Celulares/metabolismo
Fatores de Tempo
Xantenos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Indicators and Reagents); 0 (Oxazines); 0 (Xanthenes); 1FN9YD6968 (resazurin); EC 3.1.3.2 (Acid Phosphatase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-4939-6960-9_4


  6 / 3835 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29307822
[Au] Autor:Hongdan L; Feng L
[Ad] Endereço:Department of Cell Biology, Key Laboratory of Cell Biology, National Health and Family Planning Commission of the PRC, Key Laboratory of Medical Cell Biology, Ministry of Education of the PRC, China Medical University, No. 77, Puhe Road, Shenyang North New Area, Shenyang, Liaoning 110122, China.
[Ti] Título:miR-3120-5p promotes colon cancer stem cell stemness and invasiveness through targeting Axin2.
[So] Source:Biochem Biophys Res Commun;496(2):302-308, 2018 02 05.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:It is well known that colon cancer stemness and invasiveness are the main reasons for tumor recurrence and metastasis. MicroRNAs dysregulation can disrupt the balance of cell signaling and growth processes, resulting in cancer proliferation, invasion and metastasis, chemoresistance and so on. In this study, we used colon cancer cell lines HCT-116 and SW-480 to investigate the effects of miR-3120-5p on stemness and invasiveness of colon cancer. We found that the population of CD133 + and Lgr5+ stem cells in both cell lines expressed miR-3120-5p highly, and introducing miR-3120-5p into both cell lines increased the population of cancer stem cells, as measured by flow cytometry, qRT-PCR and sphere formation assays. Transwell assay, Gelatin zymography assay and Western blot assays further revealed that miR-3120-5p promotes colon cancer cells invasive ability. By the target prediction algorithm TargetScan, we found Axin2 is a potential target for miR-3120-5p, and luciferase reporter assay demonstrated that miR-3120-5p reduces Axin2 expression. Transfection of siRNA against Axin2 into colon cancer cells promoted the stemness and invasion of colon cancer cells. Furthermore, Axin2 overexpression partially reversed the promotion of stemness and invasiveness caused by miR-3120-5p in colon cancer cells. Together, all the results demonstrated miR-3120-5p promotes stemness and invasiveness of colon cancer cells through direct targeting of Axin2. They suggest that antago-miR-3120-5p plays important roles on treatment strategy for colon cancer.
[Mh] Termos MeSH primário: Proteína Axina/genética
Biomarcadores Tumorais/genética
Regulação Neoplásica da Expressão Gênica
MicroRNAs/genética
Células-Tronco Neoplásicas/metabolismo
Esferoides Celulares/metabolismo
[Mh] Termos MeSH secundário: Antígeno AC133/genética
Antígeno AC133/metabolismo
Antagomirs/genética
Antagomirs/metabolismo
Proteína Axina/antagonistas & inibidores
Proteína Axina/metabolismo
Biomarcadores Tumorais/antagonistas & inibidores
Biomarcadores Tumorais/metabolismo
Linhagem Celular Tumoral
Movimento Celular
Proliferação Celular
Genes Reporter
Células HCT116
Seres Humanos
Luciferases/genética
Luciferases/metabolismo
MicroRNAs/antagonistas & inibidores
MicroRNAs/metabolismo
Células-Tronco Neoplásicas/patologia
RNA Interferente Pequeno/genética
RNA Interferente Pequeno/metabolismo
Receptores Acoplados a Proteínas-G/genética
Receptores Acoplados a Proteínas-G/metabolismo
Transdução de Sinais
Esferoides Celulares/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (AC133 Antigen); 0 (AXIN2 protein, human); 0 (Antagomirs); 0 (Axin Protein); 0 (Biomarkers, Tumor); 0 (LGR5 protein, human); 0 (MicroRNAs); 0 (PROM1 protein, human); 0 (RNA, Small Interfering); 0 (Receptors, G-Protein-Coupled); EC 1.13.12.- (Luciferases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180109
[St] Status:MEDLINE


  7 / 3835 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29248729
[Au] Autor:Sadovska L; Zandberga E; Sagini K; Jekabsons K; Riekstina U; Kalnina Z; Llorente A; Line A
[Ad] Endereço:Latvian Biomedical Research and Study Centre, Ratsupites Str 1, k-1, LV-1067, Riga, Latvia.
[Ti] Título:A novel 3D heterotypic spheroid model for studying extracellular vesicle-mediated tumour and immune cell communication.
[So] Source:Biochem Biophys Res Commun;495(2):1930-1935, 2018 01 08.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cancer-derived extracellular vesicles (EVs) have emerged as important mediators of tumour-host interactions, and they have been shown to exert various functional effects in immune cells. In most of the studies on human immune cells, EVs have been isolated from cancer cell culture medium or patients' body fluids and added to the immune cell cultures. In such a setting, the physiological relevance of the chosen EV concentration is unknown and the EV isolation method and the timing of EV administration may bias the results. In the current study we aimed to develop an experimental cell culture model to study EV-mediated effects in human T and B cells at conditions mimicking the tumour microenvironment. We constructed a human prostate cancer cell line PC3 producing GFP-tagged EVs (PC3-CD63-GFP cells) and developed a 3D heterotypic spheroid model composed of PC3-CD63-GFP cells and human peripheral blood mononuclear cells (PBMCs). The transfer of GFP-tagged EVs from PC3-CD63-GFP cells to the lymphocytes was analysed by flow cytometry and fluorescence imaging. The endocytic pathway was investigated using three endocytosis inhibitors. Our results showed that GFP-tagged EVs interacted with a large fraction of B cells, however, the majority of EVs were not internalised by B cells but rather remained bound at the cell surface. T cell subsets differed in their ability to interact with the EVs - 15.7-24.1% of the total CD3 T cell population interacted with GFP-tagged EVs, while only 0.3-5.8% of CD8 T were GFP positive. Furthermore, a fraction of EVs were internalised in CD3 T cells via macropinocytosis. Taken together, the heterotypic PC3-CD63-GFP and PBMC spheroid model provides the opportunity to study the interactions and functional effects of cancer-derived EVs in human immune cells at conditions mimicking the tumour microenvironment.
[Mh] Termos MeSH primário: Comunicação Celular/imunologia
Técnicas de Cocultura/métodos
Vesículas Extracelulares/imunologia
Vesículas Extracelulares/patologia
Leucócitos Mononucleares/imunologia
Neoplasias Experimentais/imunologia
Esferoides Celulares/imunologia
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Seres Humanos
Leucócitos Mononucleares/patologia
Neoplasias Experimentais/patologia
Esferoides Celulares/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171218
[St] Status:MEDLINE


  8 / 3835 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29224886
[Au] Autor:Larsen CJ
[Ad] Endereço:4, rue de la Fontaine-Saint-Martin, 86110 Amberre, France. Electronic address: larsen.christian@orange.fr.
[Ti] Título:[Spheroids: A reference model for in vitro culture of solid tumors?]
[Ti] Título:Sphéroïdes : le modèle de référence pour la culture in vitro des tumeurs solides ?.
[So] Source:Bull Cancer;105(1):25-34, 2018 Jan.
[Is] ISSN:1769-6917
[Cp] País de publicação:France
[La] Idioma:fre
[Ab] Resumo:The recognition that solid tumors are complex entities composed of the tumor cell mass itself and a stromal micro-environnement providing a variety of cells from the host (fibroblasts, endothelial cells, immune cells) led to recognize that this heterogeneity could not be recapitulated in vitro by conventional bidimensional (2-D) cultures. This justified numerous attempts to develop tridimensional (3-D) cultures that provided better tools for approaching tumor complexity and more convincing drug testing systems. Among various 3-D technologies, tumor spheroids are more likely suited to provide in vitro platforms for apprehending specific aspects of different processes specifically defining each tumor category as well as testing drug delivery systems. This review summarizes current features of multicellular tumor spheroids and their suitability for studying different aspects of cancer cell biology, patient-specific therapies and drug treatment.
[Mh] Termos MeSH primário: Neoplasias/patologia
Esferoides Celulares/patologia
Microambiente Tumoral
[Mh] Termos MeSH secundário: Antineoplásicos/uso terapêutico
Movimento Celular
Proliferação Celular
Seres Humanos
Neoplasias/irrigação sanguínea
Neoplasias/tratamento farmacológico
Neoplasias/imunologia
Neovascularização Patológica
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Antineoplastic Agents)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171212
[St] Status:MEDLINE


  9 / 3835 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28452178
[Au] Autor:Matsusaki M; Komeda M; Mura S; Tanaka HY; Kano MR; Couvreur P; Akashi M
[Ad] Endereço:Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan.
[Ti] Título:Desmoplastic Reaction in 3D-Pancreatic Cancer Tissues Suppresses Molecular Permeability.
[So] Source:Adv Healthc Mater;6(15), 2017 Aug.
[Is] ISSN:2192-2659
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The survival rate of pancreatic ductal adenocarcinoma is still the lowest among all types of cancers, primarily as a consequence of an important desmoplastic reaction. Although the presence of thick stromal tissues in pancreatic tumors has been reported, in vivo animal studies do not enable a clear understanding of the crosstalk between cancer cells and fibroblasts. Accordingly, this paper reports the design and characterization of an in vitro pancreatic cancer-stromal 3D-tissue model, which enhances the understanding of the interactions between cancer cells and fibroblasts and their influence on the secretion of extracellular matrix (ECM). 3D-tissue models comprising fibroblasts and pancreatic cancer cells (MiaPaCa-2 cell line) or colon cancer cells (HT29 cell line, used as a control) show decreased molecular permeability with increased cancer cell ratios. The 3D-MiaPaCa-2 tissues display an increase in the secretion of collagen as a function of the cancer cell ratio, whereas 3D-HT29 tissues do not show a significant difference. Notably, the secretion of ECM proteins from single fibroblasts in 3D-tissue models containing 90% MiaPaCa-2 cells is ten times higher than that under 10% cancer cell conditions. In vitro pancreatic cancer 3D-tissues will be a valuable tool to obtain information on the interactions between cancer and stromal cells.
[Mh] Termos MeSH primário: Comunicação Celular
Permeabilidade da Membrana Celular
Proteínas da Matriz Extracelular/metabolismo
Neoplasias Pancreáticas/metabolismo
Esferoides Celulares/metabolismo
Células Estromais/metabolismo
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Células HT29
Seres Humanos
Neoplasias Pancreáticas/patologia
Esferoides Celulares/patologia
Células Estromais/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Extracellular Matrix Proteins)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180131
[Lr] Data última revisão:
180131
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1002/adhm.201700057


  10 / 3835 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29320969
[Au] Autor:Hachim MY; Hachim IY; Dai M; Ali S; Lebrun JJ
[Ad] Endereço:1 Cancer Research Program, Department of Medicine, McGill University Health Centre, Montreal, QC, Canada.
[Ti] Título:Differential expression of TGFß isoforms in breast cancer highlights different roles during breast cancer progression.
[So] Source:Tumour Biol;40(1):1010428317748254, 2018 Jan.
[Is] ISSN:1423-0380
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:While TGFß plays a critical role in tumor formation and progression, the role and contribution of its three different isoforms remain unclear. In this study, we aimed at elucidating the prognostic value of the TGFß isoforms and assessed their expression levels in breast cancer patients at different stages of the disease. We found higher levels of TGFß1 and TGFß3 in cancer patients compared to normal tissues, with no significant changes in TGFß2 expression. Similarly, TGFß1 and TGFß3, but not TGFß2, showed higher expression levels in advanced lymph node-positive and metastatic tumors, suggesting different roles for the different isoforms in tumor progression and the metastatic process, while in the least aggressive molecular subtype (luminal A), expression of the three TGFß isoforms significantly correlated with expression of both TGFß receptors, such correlation only occurred between TGFß1 and TGFß3 and the TGFß type II receptor (TßRII) in the highly aggressive basal-like subtype. Interestingly, a distinct and somehow opposite pattern was observed in HER-2 tumors, only showing significant association pattern between TGFß2 and the TGFß type I receptor (TßRI). Finally, the three TGFß isoforms showed distinct association patterns with patient outcome depending on the different molecular subtype, highlighting context-dependent, differential prognostic values.
[Mh] Termos MeSH primário: Neoplasias da Mama/genética
Perfilação da Expressão Gênica/métodos
Fator de Crescimento Transformador beta1/genética
Fator de Crescimento Transformador beta2/genética
Fator de Crescimento Transformador beta3/genética
[Mh] Termos MeSH secundário: Neoplasias da Mama/metabolismo
Neoplasias da Mama/patologia
Linhagem Celular Tumoral
Progressão da Doença
Feminino
Seres Humanos
Imuno-Histoquímica
Meia-Idade
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Receptores de Fatores de Crescimento Transformadores beta/genética
Receptores de Fatores de Crescimento Transformadores beta/metabolismo
Esferoides Celulares/metabolismo
Fator de Crescimento Transformador beta1/metabolismo
Fator de Crescimento Transformador beta2/metabolismo
Fator de Crescimento Transformador beta3/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Messenger); 0 (Receptors, Transforming Growth Factor beta); 0 (Transforming Growth Factor beta1); 0 (Transforming Growth Factor beta2); 0 (Transforming Growth Factor beta3)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.1177/1010428317748254



página 1 de 384 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde