Base de dados : MEDLINE
Pesquisa : A11.270 [Categoria DeCS]
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  1 / 2323 MEDLINE  
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[PMID]:29251504
[Au] Autor:Khan A; Wen Y; Huq T; Ni Y
[Ad] Endereço:Limerick Pulp and Paper Centre, Department of Chemical Engineering, University of New Brunswick , Fredericton, New Brunswick E3B 5A3, Canada.
[Ti] Título:Cellulosic Nanomaterials in Food and Nutraceutical Applications: A Review.
[So] Source:J Agric Food Chem;66(1):8-19, 2018 Jan 10.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cellulosic nanomaterials (CNMs) are organic, green nanomaterials that are obtained from renewable sources and possess exceptional mechanical strength and biocompatibility. The associated unique physical and chemical properties have made these nanomaterials an intriguing prospect for various applications including the food and nutraceutical industry. From the immobilization of various bioactive agents and enzymes, emulsion stabilization, direct food additives, to the development of intelligent packaging systems or pathogen or pH detectors, the potential food related applications for CNMs are endless. Over the past decade, there have been several reviews published covering different aspects of cellulosic nanomaterials, such as processing-structure-property relationship, physical and chemical properties, rheology, extraction, nanocomposites, etc. In this critical review, we have discussed and provided a summary of the recent developments in the utilization of cellulosic nanomaterials in applications related to food and nutraceuticals.
[Mh] Termos MeSH primário: Celulose
Suplementos Nutricionais
Indústria Alimentícia/métodos
Nanoestruturas/química
[Mh] Termos MeSH secundário: Animais
Células Imobilizadas
Emulsificantes/química
Enzimas Imobilizadas
Aditivos Alimentares/química
Embalagem de Alimentos
Seres Humanos
Mamíferos
Nanoestruturas/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Emulsifying Agents); 0 (Enzymes, Immobilized); 0 (Food Additives); 9004-34-6 (Cellulose)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04204


  2 / 2323 MEDLINE  
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[PMID]:28466286
[Au] Autor:Puza S; Gencturk E; Odabasi IE; Iseri E; Mutlu S; Ulgen KO
[Ad] Endereço:Department of Chemical Engineering, Biosystems Engineering Laboratory, Bogazici University, 34342, Istanbul, Turkey.
[Ti] Título:Fabrication of cyclo olefin polymer microfluidic devices for trapping and culturing of yeast cells.
[So] Source:Biomed Microdevices;19(2):40, 2017 Jun.
[Is] ISSN:1572-8781
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A microfluidic platform is designed and fabricated to investigate the role of uncharacterized YOR060C (Sld7) protein in aging in yeast cells for the first time. Saccharomyces cerevisiae yeast cells are trapped in the series of C-shaped regions (0.5 nL) of COP (cyclo olefin polymer), PMMA (poly methylmethacrylate), or PS (polystyrene) microbioreactors. The devices are fabricated using hot embossing and thermo-compression bonding methods. Photolithography and electrochemical etching are used to form the steel mold needed for hot embossing. The cell cycle processes are investigated by monitoring green fluorescent protein (GFP) tagged Sld7 expressions under normal as well as calorie restricted conditions. The cells are loaded at 1 µL/min flowrate and trapped successfully within each chamber. The medium is continuously fed at 0.1 µL/min throughout the experiments. Fluorescent signals of the low abundant Sld7 proteins could be distinguished only on COP devices. The background fluorescence of COP is found 1.22 and 7.24 times lower than that of PMMA, and PS, respectively. Hence, experiments are continued with COP, and lasted for more than 40 h without any contamination. The doubling time of the yeast cells are found as 72 min and 150 min, and the growth rates as 9.63 × 10 min and 4.62 × 10 min , in 2% glucose containing YPD and YNB medium, respectively. The product concentration (Sld7p:GFP) increased in accordance with cell growth. The dual role of Sld7 protein in both cell cycle and chronological aging needs to be further investigated following the preliminary experimental results.
[Mh] Termos MeSH primário: Técnicas de Cultura de Células/instrumentação
Separação Celular/instrumentação
Células Imobilizadas/citologia
Cicloparafinas/química
Dispositivos Lab-On-A-Chip
Polímeros/química
Saccharomyces cerevisiae/citologia
[Mh] Termos MeSH secundário: Reatores Biológicos
Simulação por Computador
Desenho de Equipamento
Hidrodinâmica
Microscopia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cycloparaffins); 0 (Polymers)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171225
[Lr] Data última revisão:
171225
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1007/s10544-017-0182-3


  3 / 2323 MEDLINE  
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[PMID]:28466284
[Au] Autor:Yang L; Hong T; Zhang Y; Arriola JGS; Nelms BL; Mu R; Li D
[Ad] Endereço:Department of Mechanical Engineering, Vanderbilt University, Nashville, TN, 37235, USA.
[Ti] Título:A microfluidic diode for sorting and immobilization of Caenorhabditis elegans.
[So] Source:Biomed Microdevices;19(2):38, 2017 Jun.
[Is] ISSN:1572-8781
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Caenorhabditis elegans (C. elegans) is a powerful model organism extensively used in studies of human aging and diseases. Despite the numerous advantages of C. elegans as a model system, two biological characteristics may introduce complexity and variability to most studies: 1. it exhibits different biological features, composition and behaviors at different developmental stages; 2. it has very high mobility. Therefore, synchronization and immobilization of worm populations are often required. Conventionally, these processes are implemented through manual and chemical methods, which can be laborious, time-consuming and of low-throughput. Here we demonstrate a microfluidic design capable of simultaneously sorting worms by size at a throughput of 97±4 worms per minute, and allowing for worm collection or immobilization for further investigations. The key component, a microfluidic diode structure, comprises a curved head and a straight tail, which facilitates worms to enter from the curved end but prevents them from translocating from the straight side. This design remarkably enhances the efficiency and accuracy of worm sorting at relatively low flow rates, and hence provides a practical approach to sort worms even with the presence of egg clusters and debris. In addition, we show that well-sorted worms could be immobilized, kept alive and identically orientated, which could facilitate many C. elegans-based studies.
[Mh] Termos MeSH primário: Caenorhabditis elegans/isolamento & purificação
Condutividade Elétrica
Dispositivos Lab-On-A-Chip
[Mh] Termos MeSH secundário: Animais
Células Imobilizadas
Desenho de Equipamento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171225
[Lr] Data última revisão:
171225
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1007/s10544-017-0175-2


  4 / 2323 MEDLINE  
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[PMID]:28846385
[Au] Autor:Kalaoglu-Altan OI; Kirac-Aydin A; Sumer Bolu B; Sanyal R; Sanyal A
[Ad] Endereço:Department of Chemistry and ‡Center for Life Sciences and Technologies, Bogazici University , Bebek 34342, Istanbul, Turkey.
[Ti] Título:Diels-Alder "Clickable" Biodegradable Nanofibers: Benign Tailoring of Scaffolds for Biomolecular Immobilization and Cell Growth.
[So] Source:Bioconjug Chem;28(9):2420-2428, 2017 Sep 20.
[Is] ISSN:1520-4812
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Biodegradable polymeric nanofibers have emerged as promising candidates for several biomedical applications such as tissue engineering and regenerative medicine. Many of these applications require modification of these nanofibers with small ligands or biomolecules such as peptides and other growth factors, which necessitates functionalization of these materials in mild and benign fashion. This study reports the design, synthesis, and functionalization of such nanofibers and evaluates their application as a cell culture scaffold. Polylactide based copolymers containing furan groups and triethylene glycol (TEG) units as side chains were synthesized using organocatalyzed ring opening polymerization. The furan moiety, an electron rich diene, provides "clickable" handles required for modification of nanofibers since they undergo facile cycloaddition reactions with maleimide-containing small molecules and ligands. The TEG units provide these fibers with hydrophilicity, enhanced biodegradability, and antibiofouling characteristics to minimize nonspecific adsorption. A series of copolymers with varying amounts of TEG units in their side chains were evaluated for fiber formation and antibiofouling characteristics to reveal that an incorporation of 7.5 mol % TEG-based monomer was optimal for nanofibers containing 20 mol % furan units. Facile functionalization of these nanofibers in a selective manner was demonstrated through attachment of a dienophile containing fluorophore, namely, fluorescein maleimide. To show efficient ligand-mediated bioconjugation, nanofibers were functionalized with a maleimide appended biotin, which enabled efficient attachment of the protein, Streptavidin. Importantly, the crucial role played by the TEG-based side chains was evident due to lack of any nonspecific attachment of protein to these nanofibers in the absence of biotin ligand. Furthermore, these nanofibers were conjugated with a cell adhesive cyclic peptide, cRGDfK-maleimide, at room temperature without the need of any additional catalyst. Importantly, comparison of the cell attachment onto nanofibers with and without the peptide demonstrated that fibers appended with the peptides promoted cells to spread nicely and protrude actin filaments for enhanced attachment to the support, whereas the cells on nonfunctionalized nanofibers showed a rounded up morphology with limited cellular spreading.
[Mh] Termos MeSH primário: Materiais Biocompatíveis/química
Fibroblastos/citologia
Furanos/química
Nanofibras/química
Poliésteres/química
Polietilenoglicóis/química
Tecidos Suporte/química
[Mh] Termos MeSH secundário: Animais
Materiais Biocompatíveis/síntese química
Adesão Celular
Linhagem Celular
Proliferação Celular
Células Imobilizadas/citologia
Química Click/métodos
Reação de Cicloadição/métodos
Furanos/síntese química
Camundongos
Nanofibras/ultraestrutura
Peptídeos Cíclicos/síntese química
Peptídeos Cíclicos/química
Poliésteres/síntese química
Polietilenoglicóis/síntese química
Engenharia Tecidual
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biocompatible Materials); 0 (Furans); 0 (Peptides, Cyclic); 0 (Polyesters); 0 (cyclic (arginyl-glycyl-aspartyl-phenylalanyl-lysyl)); 30IQX730WE (Polyethylene Glycols); 3P5SU53360 (triethylene glycol); 459TN2L5F5 (poly(lactide))
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171009
[Lr] Data última revisão:
171009
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170829
[St] Status:MEDLINE
[do] DOI:10.1021/acs.bioconjchem.7b00411


  5 / 2323 MEDLINE  
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[PMID]:28812894
[Au] Autor:Guo J; Zheng Z; Chen C; Lu X; Zhang Y; Zheng B
[Ad] Endereço:College of Food Science, Fujian Agriculture and Forestry University , Fuzhou, Fujian 350002, China.
[Ti] Título:Enhanced Production of κ-Carrageenase and κ-Carrageenan Oligosaccharides through Immobilization of Thalassospira sp. Fjfst-332 with Magnetic Fe O -Chitosan Microspheres.
[So] Source:J Agric Food Chem;65(36):7934-7943, 2017 Sep 13.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this study, immobilized bacteria (IMB) microsphere was prepared by embedding κ-carrageenase-producing Thalassospira sp. Fjfst-332 (TF332) onto a magnetic Fe O -chitosan carrier. The performance of Fe O -chitosan carrier was optimized by comparing its bacteria immobilization capacity at different Fe O :chitosan ratios and temperatures, while the functions of IMB microspheres were characterized by examining their κ-carrageenase production at different temperatures, pH's, and reuse cycles. At the 1:1 (w:w) Fe O :chitosan ratio, the Fe O -chitosan carriers possessed sufficient anchoring capacity for bacterial immobilization without significant compromise of their magnetism for magnetic separation of IMB from culture media. The spectroscopic analysis of IMB microspheres indicated that the immobilization of TF332 might affect the amide groups in chitosan. Compared to free bacteria, IMB can produce κ-carrageenase at higher temperature, wider pH range, and faster rate. More importantly, the κ-carrageenase-producing activity was sustained for at least seven reuse cycles. The major κ-carrageenan degradation products of IMB-derived κ-carrageenase were the oligosaccharides containing two to six monosaccharide units. Overall, this Fe O -chitosan-TF-332 microsphere has the potential to become a stable and reusable platform for large-scale production of κ-carrageenan oligosaccharides.
[Mh] Termos MeSH primário: Alphaproteobacteria/metabolismo
Proteínas de Bactérias/metabolismo
Carragenina/biossíntese
Glicosídeo Hidrolases/metabolismo
Oligossacarídeos/biossíntese
[Mh] Termos MeSH secundário: Alphaproteobacteria/química
Alphaproteobacteria/enzimologia
Células Imobilizadas/química
Células Imobilizadas/enzimologia
Células Imobilizadas/metabolismo
Quitosana/química
Meios de Cultura/química
Meios de Cultura/metabolismo
Ferro/química
Imãs/química
Sulfetos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Culture Media); 0 (Oligosaccharides); 0 (Sulfides); 0 (greigite); 9000-07-1 (Carrageenan); 9012-76-4 (Chitosan); E1UOL152H7 (Iron); EC 3.2.1.- (Glycoside Hydrolases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170817
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b02869


  6 / 2323 MEDLINE  
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[PMID]:28778038
[Au] Autor:Zang Y; Guo N; Jiao J; Wang X; Gai Q; Xu W; Fu Y
[Ad] Endereço:Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China; Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China.
[Ti] Título:Application of magnetically immobilized edible fungus for the biotransformation of panax notoginseng saponin Rb1 to Rd and Rg3.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1061-1062:306-313, 2017 Sep 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In this study, we developed a new magnetically immobilized edible fungus for the biotransformation of panax notoginseng saponins Rb1 to Rd and Rg3. The optimum biotransformation conditions were as follows: temperature 32°C, pH 6.5, time 48h and liquid-solid ratio 25:1(mL/g). The yields of Rd and Rg3 reached 41.35±0.12mg/g and 6.35±0.08mg/g which increased 6.97-fold and 3.23-fold to that of untreated control, respectively. Additionally, SEM demonstrated that vestured pits and cell walls of samples were destroyed obviously which was beneficial to the Rb1 biotransformation and target Rd and Rg3 release. Meanwhile, the reusability of magnetically immobilized microorganism was tested and the activity of the magnetically immobilized microorganism remained 83.8% after 15 runs. The recycling experiments demonstrated that magnetically immobilized fungus exhibited higher efficiency than the non-magnetical one. These results proved that this new magnetically immobilized microorganism could be applied for industrial production and pharmaceutical industry with good efficiency.
[Mh] Termos MeSH primário: Células Imobilizadas/metabolismo
Medicamentos de Ervas Chinesas/química
Óxido Ferroso-Férrico/química
Fungos
Ginsenosídeos
Panax notoginseng
[Mh] Termos MeSH secundário: Biotecnologia/métodos
Medicamentos de Ervas Chinesas/metabolismo
Fungos/citologia
Fungos/enzimologia
Fungos/metabolismo
Ginsenosídeos/análise
Ginsenosídeos/química
Ginsenosídeos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drugs, Chinese Herbal); 0 (Ginsenosides); XM0M87F357 (Ferrosoferric Oxide)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170805
[St] Status:MEDLINE


  7 / 2323 MEDLINE  
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[PMID]:28726102
[Au] Autor:Maksimova YG; Gorbunova AN; Demakov VA
[Ad] Endereço:Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences, Perm, 614081, Russia. maks@iegm.ru.
[Ti] Título:Stereoselective biotransformation of phenylglycine nitrile by heterogeneous biocatalyst based on immobilized bacterial cells and enzyme preparation.
[So] Source:Dokl Biochem Biophys;474(1):183-185, 2017 May.
[Is] ISSN:1608-3091
[Cp] País de publicação:Russia (Federation)
[La] Idioma:eng
[Ab] Resumo:We studied the effect of a heterogeneous environment on the stereoselectivity of transformation of racemic phenylglycine nitrile. Immobilized biocatalysts were prepared by adhesion of Pseudomonas fluorescens C2 cells on carbon-containing supports and covalent crosslinking of nitrile hydratase and amidase of Rhodococcus rhodochrous 4-1 to activated chitosan as well as by the method of cross-linked aggregates. At a reaction duration of 20 h, the ratio of phenylglycine stereoisomers changes depending on the presence of support in medium. The highest optical purity of the product (enantiomeric excess of L-phenylglycine solution, 98%) is achieved when enzyme aggregates of nitrile hydratase and amidase cross-linked with 0.1% glutaraldehyde are used as a biocatalyst.
[Mh] Termos MeSH primário: Acetonitrilos/química
Acetonitrilos/metabolismo
Amidoidrolases/metabolismo
Biocatálise
Hidroliases/metabolismo
Pseudomonas/citologia
[Mh] Termos MeSH secundário: Amidoidrolases/química
Aderência Bacteriana
Biotransformação
Células Imobilizadas/citologia
Hidroliases/química
Hidrólise
Rhodococcus/enzimologia
Estereoisomerismo
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetonitriles); 0 (phenylglycine nitrile); EC 3.5.- (Amidohydrolases); EC 3.5.1.4 (amidase); EC 4.2.1.- (Hydro-Lyases); EC 4.2.1.- (nitrile hydratase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170721
[St] Status:MEDLINE
[do] DOI:10.1134/S1607672917030139


  8 / 2323 MEDLINE  
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[PMID]:28606145
[Au] Autor:Liu S; Liu J; Hou J; Chao N; Gai Y; Jiang X
[Ad] Endereço:College of Biological Science and Technology, Beijing Forestry University, Beijing, 100083, People's Republic of China.
[Ti] Título:Three steps in one pot: biosynthesis of 4-hydroxycinnamyl alcohols using immobilized whole cells of two genetically engineered Escherichia coli strains.
[So] Source:Microb Cell Fact;16(1):104, 2017 Jun 12.
[Is] ISSN:1475-2859
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: 4-Hydroxycinnamyl alcohols are a class of natural plant secondary metabolites that include p-coumaryl alcohol, caffeyl alcohol, coniferyl alcohol and sinapyl alcohol, and have physiological, ecological and biomedical significance. While it is necessary to investigate the biological pathways and economic value of these alcohols, research is hindered because of their limited availability and high cost. Traditionally, these alcohols are obtained by chemical synthesis and plant extraction. However, synthesis by biotransformation with immobilized microorganisms is of great interest because it is environmentally friendly and offers high stability and regenerable cofactors. Therefore, we produced 4-hydroxycinnamyl alcohols using immobilized whole cells of engineered Escherichia coli as the biocatalyst. RESULTS: In this study, we used the recombinant E. coli strain, M15-4CL1-CCR, expressing the fusion protein 4-coumaric acid: coenzyme A ligase and the cinnamoyl coenzyme A reductase and a recombinant E. coli strain, M15-CAD, expressing cinnamyl alcohol dehydrogenase from Populus tomentosa (P. tomentosa). High performance liquid chromatography and mass spectrometry showed that the immobilized whole cells of the two recombinant E. coli strains could effectively convert the phenylpropanoic acids to their corresponding 4-hydroxycinnamyl alcohols. Further, the optimum buffer pH and the reaction temperature were pH 7.0 and 30 °C. Under these conditions, the molar yield of the p-coumaryl alcohol, the caffeyl alcohol and the coniferyl alcohol was around 58, 24 and 60%, respectively. Moreover, the highly sensitive and selective HPLC-PDA-ESI-MSn method used in this study could be applied to the identification and quantification of these aromatic polymers. CONCLUSIONS: We have developed a dual-cell immobilization system for the production of 4-hydroxycinnamyl alcohols from inexpensive phenylpropanoic acids. This biotransformation method is both simple and environmental-friendly, which is promising for the practical and cost effective synthesis of natural products. Graphical abstract Biotransformation process of phenylpropanoic acids by immobilized whole-cells.
[Mh] Termos MeSH primário: Oxirredutases do Álcool/metabolismo
Escherichia coli/genética
Escherichia coli/metabolismo
Engenharia Genética
Propanóis/metabolismo
[Mh] Termos MeSH secundário: Vias Biossintéticas
Células Imobilizadas/metabolismo
Escherichia coli/citologia
Propanóis/química
Proteínas Recombinantes/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Propanols); 0 (Recombinant Proteins); EC 1.1.- (Alcohol Oxidoreductases); EC 1.1.1.195 (cinnamyl alcohol dehydrogenase); SS8YOP444F (cinnamyl alcohol)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170614
[St] Status:MEDLINE
[do] DOI:10.1186/s12934-017-0722-9


  9 / 2323 MEDLINE  
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[PMID]:28581736
[Au] Autor:Simó G; Vila-Crespo J; Fernández-Fernández E; Ruipérez V; Rodríguez-Nogales JM
[Ad] Endereço:Food Technology Area, University of Valladolid, Technical High School of Agronomic Engineering , Av. Madrid 44, 34071 Palencia, Spain.
[Ti] Título:Highly Efficient Malolactic Fermentation of Red Wine Using Encapsulated Bacteria in a Robust Biocomposite of Silica-Alginate.
[So] Source:J Agric Food Chem;65(25):5188-5197, 2017 Jun 28.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bacteria encapsulation to develop malolactic fermentation emerges as a biotechnological strategy that provides significant advantages over the use of free cells. Two encapsulation methods have been proposed embedding Oenococcus oeni, (i) interpenetrated polymer networks of silica and Ca-alginate and (ii) Ca-alginate capsules coated with hydrolyzed 3-aminopropyltriethoxysilane (hAPTES). On the basis of our results, only the first method was suitable for bacteria encapsulation. The optimized silica-alginate capsules exhibited a negligible bacteria release and an increase of 328% and 65% in L-malic acid consumption and mechanical robustness, respectively, compared to untreated alginate capsules. Moreover, studies of capsule stability at different pH and ethanol concentrations in water solutions and in wine indicated a better behavior of silica-alginate capsules than untreated ones. The inclusion of silicates and colloidal silica in alginate capsules containing O. oeni improved markedly their capacity to deplete the levels of L-malic acid in red wines and their mechanical robustness and stability.
[Mh] Termos MeSH primário: Oenococcus/química
Vitis/microbiologia
Vinho/microbiologia
[Mh] Termos MeSH secundário: Alginatos/química
Células Imobilizadas/química
Fermentação
Ácido Glucurônico/química
Ácidos Hexurônicos/química
Ácido Láctico/metabolismo
Malatos/metabolismo
Oenococcus/metabolismo
Dióxido de Silício/química
Vitis/metabolismo
Vinho/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alginates); 0 (Hexuronic Acids); 0 (Malates); 33X04XA5AT (Lactic Acid); 7631-86-9 (Silicon Dioxide); 817L1N4CKP (malic acid); 8A5D83Q4RW (Glucuronic Acid); 8C3Z4148WZ (alginic acid)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170707
[Lr] Data última revisão:
170707
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170606
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b01210


  10 / 2323 MEDLINE  
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[PMID]:28525799
[Au] Autor:Yu L; Yuan Y; Tang J; Zhou S
[Ad] Endereço:Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
[Ti] Título:Thermophilic Moorella thermoautotrophica-immobilized cathode enhanced microbial electrosynthesis of acetate and formate from CO .
[So] Source:Bioelectrochemistry;117:23-28, 2017 Oct.
[Is] ISSN:1878-562X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Microbial electrosynthesis (MES) is a promising technique that converts electricity and CO to biofuels using microbes as the catalysts. However, most of previous MES are conducted at mesophilic temperatures and challenged by low performances. Here we report a significant electrosynthesis performance enhancement via immobilization of a thermophilic microbe to cathodes. A temperature-dependent electron uptake rate of Moorella thermoautotrophica was observed at a cathode potential of -0.4V (vs. SHE), with a maximum current density of 63.47mAm at 55°C. Moreover, electrosynthesis rates of formate and acetate at 55°C were accelerated by 23.2 and 2.8 fold than those of 25°C, respectively. Compared with natural biofilms, immobilization of M. thermoautotrophica with carbon nanoparticles to electrodes further enhanced acetate and formate production rates (by 14 and 7.9 fold), reaching 58.2 and 63.2mmolm day at a coulombic efficiency of 65%, respectively. To our best knowledge, these are the highest electrosynthesis rates obtained thus far for pure cultures under the conditions of -0.4V (vs. SHE) and 55°C. This study, for the first time, demonstrates that embedding microbes to electrodes by carbon nanoparticles is a facile and efficient method of improving MES performance.
[Mh] Termos MeSH primário: Acetatos/metabolismo
Fontes de Energia Bioelétrica/microbiologia
Dióxido de Carbono/metabolismo
Formiatos/metabolismo
Moorella/metabolismo
[Mh] Termos MeSH secundário: Células Imobilizadas/metabolismo
Eletroquímica
Moorella/citologia
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetates); 0 (Formates); 0YIW783RG1 (formic acid); 142M471B3J (Carbon Dioxide)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170815
[Lr] Data última revisão:
170815
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170520
[St] Status:MEDLINE



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