[PMID]: | 26996782 |
[Au] Autor: | Calvet CM; Silva TA; DE Melo TG; DE Araújo-Jorge TC; Pereira MC |
[Ad] Endereço: | Laboratório de Ultraestrutura Celular,Fundação Oswaldo Cruz,Av. Brasil 4365,Pav. Carlos Chagas 3° andar,Rio de Janeiro,Brazil. |
[Ti] Título: | TGF-ß receptor type II costameric localization in cardiomyocytes and host cell TGF-ß response is disrupted by Trypanosoma cruzi infection. |
[So] Source: | Parasitology;143(6):704-15, 2016 05. |
[Is] ISSN: | 1469-8161 |
[Cp] País de publicação: | England |
[La] Idioma: | eng |
[Ab] Resumo: | Transforming growth factor beta (TGF-ß) cytokine is involved in Chagas disease establishment and progression. Since Trypanosoma cruzi can modulate host cell receptors, we analysed the TGF-ß receptor type II (TßRII) expression and distribution during T. cruzi - cardiomyocyte interaction. TßRII immunofluorescent staining revealed a striated organization in cardiomyocytes, which was co-localized with vinculin costameres and enhanced (38%) after TGF-ß treatment. Cytochalasin D induced a decrease of 45·3% in the ratio of cardiomyocytes presenting TßRII striations, demonstrating an association of TßRII with the cytoskeleton. Western blot analysis showed that cytochalasin D significantly inhibited Smad 2 phosphorylation and fibronectin stimulation after TGF-ß treatment in cardiomyocytes. Trypanosoma cruzi infection elicited a decrease of 79·8% in the frequency of cardiomyocytes presenting TßRII striations, but did not interfere significantly in its expression. In addition, T. cruzi-infected cardiomyocytes present a lower response to exogenous TGF-ß, showing no enhancement of TßRII striations and a reduction of phosphorylated Smad 2, with no significant difference in TßRII expression when compared to uninfected cells. Together, these results suggest that the co-localization of TßRII with costameres is important in activating the TGF-ß signalling cascade, and that T. cruzi-derived cytoskeleton disorganization could result in altered or low TGF-ß response in infected cardiomyocytes. |
[Mh] Termos MeSH primário: |
Doença de Chagas/fisiopatologia Costâmeros/metabolismo Interações Hospedeiro-Parasita/fisiologia Miócitos Cardíacos/patologia Proteínas Serina-Treonina Quinases/metabolismo Receptores de Fatores de Crescimento Transformadores beta/metabolismo
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[Mh] Termos MeSH secundário: |
Animais Células Cultivadas Regulação da Expressão Gênica/efeitos dos fármacos Interações Hospedeiro-Parasita/efeitos dos fármacos Camundongos Miócitos Cardíacos/parasitologia Transporte Proteico/efeitos dos fármacos Transporte Proteico/fisiologia Transdução de Sinais/efeitos dos fármacos Fator de Crescimento Transformador beta/metabolismo Fator de Crescimento Transformador beta/farmacologia Trypanosoma cruzi/fisiologia
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[Pt] Tipo de publicação: | JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T |
[Nm] Nome de substância:
| 0 (Receptors, Transforming Growth Factor beta); 0 (Transforming Growth Factor beta); EC 2.7.11.1 (Protein-Serine-Threonine Kinases); EC 2.7.11.30 (transforming growth factor-beta type II receptor) |
[Em] Mês de entrada: | 1704 |
[Cu] Atualização por classe: | 170407 |
[Lr] Data última revisão:
| 170407 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 160322 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1017/S0031182016000299 |
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