Base de dados : MEDLINE
Pesquisa : A11.284.180.690 [Categoria DeCS]
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[PMID]:26185067
[Au] Autor:Antonova ES; Hammer BK
[Ti] Título:Genetics of Natural Competence in Vibrio cholerae and other Vibrios.
[So] Source:Microbiol Spectr;3(3), 2015 Jun.
[Is] ISSN:2165-0497
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Many Gram-positive and Gram-negative bacteria can become naturally competent to take up extracellular DNA from the environment via a dedicated uptake apparatus. The genetic material that is acquired can (i) be used for nutrients, (ii) aid in genome repair, and (iii) promote horizontal gene transfer when incorporated onto the genome by homologous recombination, the process of "transformation." Recent studies have identified multiple environmental cues sufficient to induce natural transformation in Vibrio cholerae and several other Vibrio species. In V. cholerae, nutrient limitation activates the cAMP receptor protein regulator, quorum-sensing signals promote synthesis of HapR-controlled QstR, chitin stimulates production of TfoX, and low extracellular nucleosides allow CytR to serve as an additional positive regulator. The network of signaling systems that trigger expression of each of these required regulators is well described, but the mechanisms by which each in turn controls competence apparatus genes is poorly understood. Recent work has defined a minimal set of genes that encode apparatus components and begun to characterize the architecture of the machinery by fluorescence microscopy. While studies with a small set of V. cholerae reference isolates have identified regulatory and competence genes required for DNA uptake, future studies may identify additional genes and regulatory connections, as well as revealing how common natural competence is among diverse V. cholerae isolates and other Vibrio species.
[Mh] Termos MeSH primário: Competência de Transformação por DNA/genética
DNA Bacteriano/genética
Transferência Genética Horizontal/genética
Vibrio cholerae/genética
[Mh] Termos MeSH secundário: Transporte Biológico/genética
Quitina/metabolismo
Proteína Receptora de AMP Cíclico/metabolismo
DNA/metabolismo
Proteínas de Ligação a DNA/genética
Ativação Enzimática
Proteínas de Fímbrias/genética
Regulação Bacteriana da Expressão Gênica
Pili Sexual/genética
Percepção de Quorum/genética
Percepção de Quorum/fisiologia
Transdução de Sinais/genética
Vibrio cholerae/patogenicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cyclic AMP Receptor Protein); 0 (DNA, Bacterial); 0 (DNA-Binding Proteins); 1398-61-4 (Chitin); 147680-16-8 (Fimbriae Proteins); 9007-49-2 (DNA)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:150718
[Lr] Data última revisão:
150718
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150718
[St] Status:MEDLINE
[do] DOI:10.1128/microbiolspec.VE-0010-2014


  2 / 171 MEDLINE  
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[PMID]:24899524
[Au] Autor:Wright CJ; Wu H; Melander RJ; Melander C; Lamont RJ
[Ad] Endereço:Oral Health and Systemic Disease, University of Louisville, Louisville, KY, USA.
[Ti] Título:Disruption of heterotypic community development by Porphyromonas gingivalis with small molecule inhibitors.
[So] Source:Mol Oral Microbiol;29(5):185-93, 2014 Oct.
[Is] ISSN:2041-1014
[Cp] País de publicação:Denmark
[La] Idioma:eng
[Ab] Resumo:Porphyromonas gingivalis is one of the main etiological organisms in periodontal disease. On oral surfaces P. gingivalis is a component of multispecies biofilm communities and can modify the pathogenic potential of the community as a whole. Accumulation of P. gingivalis in communities is facilitated by interspecies binding and communication with the antecedent colonizer Streptococcus gordonii. In this study we screened a library of small molecules to identify structures that could serve as lead compounds for the development of inhibitors of P. gingivalis community development. Three small molecules were identified that effectively inhibited accumulation of P. gingivalis on a substratum of S. gordonii. The structures of the small molecules are derived from the marine alkaloids oroidin and bromoageliferin and contain a 2-aminoimidazole or 2-aminobenzimidazole moiety. The most active compounds reduced expression of mfa1 and fimA in P. gingivalis, genes encoding the minor and major fimbrial subunits, respectively. These fimbrial adhesins are necessary for P. gingivalis co-adhesion with S. gordonii. These results demonstrate the potential for a small molecular inhibitor-based approach to the prevention of diseases associated with P. gingivalis.
[Mh] Termos MeSH primário: Aderência Bacteriana/efeitos dos fármacos
Biofilmes/efeitos dos fármacos
Porphyromonas gingivalis/efeitos dos fármacos
Bibliotecas de Moléculas Pequenas
[Mh] Termos MeSH secundário: Proteínas de Bactérias/antagonistas & inibidores
Proteínas de Bactérias/efeitos dos fármacos
Benzimidazóis/farmacologia
Liases de Carbono-Enxofre/efeitos dos fármacos
Proteínas de Fímbrias/antagonistas & inibidores
Seres Humanos
Processamento de Imagem Assistida por Computador/métodos
Imagem Tridimensional/métodos
Imidazóis/farmacologia
Interações Microbianas
Microscopia Confocal/métodos
Pili Sexual/efeitos dos fármacos
Porphyromonas gingivalis/fisiologia
Pirróis/farmacologia
Streptococcus gordonii/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Benzimidazoles); 0 (Imidazoles); 0 (Mfa1 protein, Porphyromonas gingivalis); 0 (Pyrroles); 0 (Small Molecule Libraries); 0 (ageliferin); 0 (fimbrillin); 147680-16-8 (Fimbriae Proteins); 7720-39-0 (2-aminoimidazole); E65DE7521V (2-aminobenzimidazole); EC 4.4.- (Carbon-Sulfur Lyases); EC 4.4.1.21 (LuxS protein, Bacteria); PF75E92XKM (oroidin)
[Em] Mês de entrada:1504
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:140606
[St] Status:MEDLINE
[do] DOI:10.1111/omi.12060


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[PMID]:24797914
[Au] Autor:Vik Å; Anonsen JH; Aas FE; Hegge FT; Roos N; Koomey M; Aspholm M
[Ad] Endereço:Centre for Molecular Biology and Neuroscience, University of Oslo, Oslo, Norway; Department of Biosciences, University of Oslo, Oslo, Norway.
[Ti] Título:Type IV pilus assembly proficiency and dynamics influence pilin subunit phospho-form macro- and microheterogeneity in Neisseria gonorrhoeae.
[So] Source:PLoS One;9(5):e96419, 2014.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The PilE pilin subunit protein of the gonococcal Type IV pilus (Tfp) colonization factor undergoes multisite, covalent modification with the zwitterionic phospho-form modification phosphoethanolamine (PE). In a mutant lacking the pilin-like PilV protein however, PilE is modified with a mixture of PE and phosphocholine (PC). Moreover, intrastrain variation of PilE PC modification levels have been observed in backgrounds that constitutively express PptA (the protein phospho-form transferase A) required for both PE and PC modification. The molecular basis underlying phospho-form microheterogeneity in these instances remains poorly defined. Here, we examined the effects of mutations at numerous loci that disrupt or perturb Tfp assembly and observed that these mutants phenocopy the pilV mutant vis a vis phospho-form modification status. Thus, PC modification appears to be directly or indirectly responsive to the efficacy of pilin subunit interactions. Despite the complexity of contributing factors identified here, the data favor a model in which increased retention in the inner membrane may act as a key signal in altering phospho-form modification. These results also provide an alternative explanation for the variation in PilE PC levels observed previously and that has been assumed to be due to phase variation of pptA. Moreover, mass spectrometry revealed evidence for mono- and di-methylated forms of PE attached to PilE in mutants deficient in pilus assembly, directly implicating a methyltransferase-based pathway for PC synthesis in N. gonorrhoeae.
[Mh] Termos MeSH primário: Proteínas de Fímbrias/metabolismo
Neisseria gonorrhoeae/metabolismo
[Mh] Termos MeSH secundário: Cromatografia Líquida
Eletroforese em Gel de Poliacrilamida
Etanolaminas/química
Etanolaminas/metabolismo
Proteínas de Fímbrias/química
Proteínas de Fímbrias/genética
Regulação Bacteriana da Expressão Gênica
Glicosilação
Immunoblotting
Espectrometria de Massas
Modelos Moleculares
Mutação de Sentido Incorreto
Fosforilcolina/química
Fosforilcolina/metabolismo
Pili Sexual/metabolismo
Processamento de Proteína Pós-Traducional
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Ethanolamines); 0 (pilE protein, Neisseria gonorrhoeae); 107-73-3 (Phosphorylcholine); 147680-16-8 (Fimbriae Proteins); 78A2BX7AEU (phosphorylethanolamine)
[Em] Mês de entrada:1507
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140507
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0096419


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[PMID]:24147843
[Au] Autor:Zeller I; Hutcherson JA; Lamont RJ; Demuth DR; Gumus P; Nizam N; Buduneli N; Scott DA
[Ad] Endereço:Oral Health and Systemic Disease, University of Louisville, Louisville, KY.
[Ti] Título:Altered antigenic profiling and infectivity of Porphyromonas gingivalis in smokers and non-smokers with periodontitis.
[So] Source:J Periodontol;85(6):837-44, 2014 Jun.
[Is] ISSN:1943-3670
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Cigarette smokers are more susceptible to periodontal diseases and are more likely to be infected with Porphyromonas gingivalis than non-smokers. Furthermore, smoking is known to alter the expression of P. gingivalis surface components and compromise immunoglobulin (Ig)G generation. The aim of this study is to evaluate whether the overall IgG response to P. gingivalis is suppressed in smokers in vivo and whether previously established in vitro tobacco-induced phenotypic P. gingivalis changes would be reflected in vivo. METHODS: The authors examined the humoral response to several P. gingivalis strains as well as specific tobacco-regulated outer membrane proteins (FimA and RagB) by enzyme-linked immunosorbent assay in biochemically validated (salivary cotinine) smokers and non-smokers with chronic periodontitis (CP: n = 13) or aggressive periodontitis (AgP: n = 20). The local and systemic presence of P. gingivalis DNA was also monitored by polymerase chain reaction. RESULTS: Smoking was associated with decreased total IgG responses against clinical (10512, 5607, and 10208C; all P <0.05) but not laboratory (ATCC 33277, W83) P. gingivalis strains. Smoking did not influence IgG produced against specific cell-surface proteins, although a non-significant pattern toward increased total FimA-specific IgG in patients with CP, but not AgP, was observed. Seropositive smokers were more likely to be infected orally and systemically with P. gingivalis (P <0.001), as determined by 16S RNA analysis. CONCLUSION: Smoking alters the humoral response against P. gingivalis and may increase P. gingivalis infectivity, strengthening the evidence that mechanisms of periodontal disease progression in smokers may differ from those of non-smokers with the same disease classification.
[Mh] Termos MeSH primário: Periodontite Agressiva/microbiologia
Antígenos de Bactérias/imunologia
Periodontite Crônica/microbiologia
Porphyromonas gingivalis/imunologia
Fumar/imunologia
[Mh] Termos MeSH secundário: Adulto
Periodontite Agressiva/imunologia
Anticorpos Antibacterianos/imunologia
Proteínas de Bactérias/imunologia
Infecções por Bacteroidaceae/imunologia
Infecções por Bacteroidaceae/microbiologia
Periodontite Crônica/imunologia
Cotinina/análise
DNA Bacteriano/análise
Índice de Placa Dentária
Feminino
Proteínas de Fímbrias/imunologia
Seres Humanos
Imunoglobulina G/imunologia
Masculino
Meia-Idade
Índice Periodontal
Fenótipo
Pili Sexual/imunologia
Porphyromonas gingivalis/genética
Porphyromonas gingivalis/patogenicidade
Saliva/química
Tabaco
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Antibodies, Bacterial); 0 (Antigens, Bacterial); 0 (Bacterial Proteins); 0 (DNA, Bacterial); 0 (Immunoglobulin G); 0 (Mfa1 protein, Porphyromonas gingivalis); 0 (RagB protein, bacteria); 0 (fimbrillin); 147680-16-8 (Fimbriae Proteins); K5161X06LL (Cotinine)
[Em] Mês de entrada:1504
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:131024
[St] Status:MEDLINE
[do] DOI:10.1902/jop.2013.130336


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[PMID]:23646850
[Au] Autor:Feng X; Zhang L; Xu L; Meng H; Lu R; Chen Z; Shi D; Wang X
[Ad] Endereço:Department of Periodontology, Peking University School and Hospital of Stomatology, Haidian District, Beijing, China.
[Ti] Título:Detection of eight periodontal microorganisms and distribution of Porphyromonas gingivalis fimA genotypes in Chinese patients with aggressive periodontitis.
[So] Source:J Periodontol;85(1):150-9, 2014 Jan.
[Is] ISSN:1943-3670
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The microbiologic feature of aggressive periodontitis (AgP) in Chinese patients has not yet been determined. This study aims to investigate the prevalence of eight periodontal microorganisms and the distribution of the Porphyromonas gingivalis fimA genotype in a cohort of Chinese patients with AgP. METHODS: Saliva and pooled subgingival plaque samples were collected from 81 patients with AgP (25 with incisor-first molar type and 56 with generalized type [GAgP]) and 34 periodontally healthy controls. Eight periodontal microorganisms, including Aggregatibacter actinomycetemcomitans, P. gingivalis, Tannerella forsythia, Treponema denticola, Campylobacter rectus, Prevotella intermedia, Prevotella nigrescens, and Fusobacterium nucleatum were detected in these samples by the polymerase chain reaction (PCR). In addition, the distribution of fimA genotypes was assessed in P. gingivalis-positive individuals by PCR. RESULTS: The prevalence of P. gingivalis, T. forsythia, T. denticola, C. rectus, P. intermedia, F. nucleatum, and A. actinomycetemcomitans in patients with AgP was significantly higher than that in healthy controls. The prevalence of A. actinomycetemcomitans in patients with GAgP was relatively low (30.4%) compared with other pathogens. Results of logistic regression analysis showed that younger patients were more likely to harbor A. actinomycetemcomitans (odds ratio = 2.85). Type II was the most prevalent fimA genotype of P. gingivalis in patients with AgP. CONCLUSIONS: P. gingivalis, T. forsythia, T. denticola, C. rectus, P. intermedia, and F. nucleatum were the predominant periodontal pathogens of patients with GAgP in China. Type II of fimA was the most prevalent genotype of P. gingivalis in patients with AgP. The prevalence of A. actinomycetemcomitans in patients with GAgP was relatively low.
[Mh] Termos MeSH primário: Periodontite Agressiva/microbiologia
Bactérias/classificação
Proteínas de Fímbrias/genética
Pili Sexual/genética
Porphyromonas gingivalis/classificação
[Mh] Termos MeSH secundário: Adulto
Fatores Etários
Aggregatibacter actinomycetemcomitans/isolamento & purificação
Periodontite Agressiva/classificação
Carga Bacteriana
Bacteroides/isolamento & purificação
Campylobacter rectus/isolamento & purificação
Estudos de Coortes
Placa Dentária/microbiologia
Feminino
Fusobacterium nucleatum/isolamento & purificação
Genótipo
Seres Humanos
Masculino
Meia-Idade
Prevotella intermedia/isolamento & purificação
Prevotella nigrescens/isolamento & purificação
Saliva/microbiologia
Treponema denticola/isolamento & purificação
Adulto Jovem
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (fimbrillin); 147680-16-8 (Fimbriae Proteins)
[Em] Mês de entrada:1411
[Cu] Atualização por classe:131224
[Lr] Data última revisão:
131224
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:130508
[St] Status:MEDLINE
[do] DOI:10.1902/jop.2013.120677


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[PMID]:23447375
[Au] Autor:Mahendra J; Mahendra L; Felix J; Romanos GE
[Ad] Endereço:Department of Periodontology, Meenakshi Ammal Dental College, Meenakshi Academy of Higher Education and Research, Chennai, India.
[Ti] Título:Genetic analysis of Porphyromonas gingivalis (fimA), Aggregatibacter actinomycetemcomitans, and red complex in coronary plaque.
[So] Source:J Investig Clin Dent;5(3):201-7, 2014 Aug.
[Is] ISSN:2041-1626
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:AIM: The objective of the present study was to detect the presence of Porphyromonas gingivalis (fimA), Aggregatibacter actinomycetemcomitans, and red complex in the coronary plaque of patients with coronary artery disease. METHODS: The study population consisted of 51 patients with chronic periodontitis undergoing coronary artery bypass grafting. DNA was extracted from subgingival and coronary atherosclerotic plaque samples. Polymerase chain reaction was used to amplify the part of 16S rRNA gene to detect the presence of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis (fimA), Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola. RESULTS: Aggregatibacter actinomycetemcomitans, Tannerella forsythia, Porphyromonas gingivalis, Porphyromonas gingivalis (fimA), and Treponema denticola were detected in 0%, 31.4%, 45.1%, 39.2%, and 51% of the atherosclerotic plaque samples, respectively. In both subgingival and coronary atherosclerotic plaque samples, Tannerella forsythia was detected in 19.6%, Porphyromonas gingivalis in 39.2%, Porphyromonas gingivalis (fimA) in 33.3%, and Treponema denticola in 35.3% of the samples. CONCLUSION: The study confirmed the detection of red complex bacteria in coronary plaque samples. However Aggregatibacter actinomycetemcomitans could not be detected in these samples.
[Mh] Termos MeSH primário: Aggregatibacter actinomycetemcomitans/genética
Doença da Artéria Coronariana/microbiologia
Proteínas de Fímbrias/genética
Pili Sexual/genética
Placa Aterosclerótica/microbiologia
Porphyromonas gingivalis/genética
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Aggregatibacter actinomycetemcomitans/classificação
Bacteroides/classificação
Bacteroides/genética
Periodontite Crônica/complicações
Periodontite Crônica/microbiologia
Ponte de Artéria Coronária
Doença da Artéria Coronariana/cirurgia
DNA Bacteriano/análise
Placa Dentária/microbiologia
Índice de Placa Dentária
Feminino
Proteínas de Fímbrias/classificação
Seres Humanos
Masculino
Meia-Idade
Pili Sexual/classificação
Placa Aterosclerótica/cirurgia
Reação em Cadeia da Polimerase
Porphyromonas gingivalis/classificação
RNA Bacteriano/análise
RNA Ribossômico 16S/análise
Treponema denticola/classificação
Treponema denticola/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Bacterial); 0 (RNA, Ribosomal, 16S); 0 (fimbrillin); 147680-16-8 (Fimbriae Proteins)
[Em] Mês de entrada:1506
[Cu] Atualização por classe:140807
[Lr] Data última revisão:
140807
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:130301
[St] Status:MEDLINE
[do] DOI:10.1111/jicd.12030


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[PMID]:24203442
[Au] Autor:Munson GP
[Ad] Endereço:Department of Microbiology and Immunology, University of Miami Miller School of Medicine, P.O. Box 016960 (R-138), Miami, FL, 33101, USA, gmunson@miami.edu.
[Ti] Título:Virulence regulons of enterotoxigenic Escherichia coli.
[So] Source:Immunol Res;57(1-3):229-36, 2013 Dec.
[Is] ISSN:1559-0755
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Enterotoxigenic Escherichia coli is frequently associated with travelers' diarrhea and is a leading cause of infant and childhood mortality in developing countries. Disease is dependent upon the orchestrated expression of enterotoxins, flexible adhesive pili, and other virulence factors. Both the heat-labile (LT) and heat-stable (ST-H) enterotoxins are regulated at the level of transcription by cAMP-receptor protein which represses the expression of LT while activating expression of ST-H. The expression of many different serotypes of adhesive pili is regulated by Rns, a member of the AraC family that represents a subgroup of conserved virulence regulators from several enteric pathogens. These Rns-like regulators recognize similar DNA binding sites, and a compiled sequence logo suggests they may bind DNA through both major and minor groove interactions. These regulators are also tempting targets for novel therapeutics because they play pivotal roles during infection. To that end, high-throughput screens have begun to identify compounds that inhibit the activity of these regulators, predominately by interfering with DNA binding.
[Mh] Termos MeSH primário: Escherichia coli Enterotoxigênica/genética
Escherichia coli Enterotoxigênica/patogenicidade
Regulon/genética
[Mh] Termos MeSH secundário: Animais
Aderência Bacteriana/genética
Sítios de Ligação
Proteínas de Ligação a DNA/química
Proteínas de Ligação a DNA/genética
Proteínas de Ligação a DNA/metabolismo
Diarreia/microbiologia
Escherichia coli Enterotoxigênica/metabolismo
Enterotoxinas/genética
Enterotoxinas/metabolismo
Seres Humanos
Pili Sexual/genética
Pili Sexual/metabolismo
Domínios e Motivos de Interação entre Proteínas
Transativadores/química
Transativadores/genética
Transativadores/metabolismo
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; REVIEW
[Nm] Nome de substância:
0 (DNA-Binding Proteins); 0 (Enterotoxins); 0 (Rns protein, bacteria); 0 (Trans-Activators)
[Em] Mês de entrada:1407
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131109
[St] Status:MEDLINE
[do] DOI:10.1007/s12026-013-8453-4


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[PMID]:23855174
[Au] Autor:Martinez-Martinez RE; Loyola-Rodriguez JP; Bonilla-Garro SE; Patiño-Marin N; Haubek D; Amano A; Poulsen K
[Ad] Endereço:San Luis Potosi University, Mexico.
[Ti] Título:Characterization of periodontal biofilm in Down syndrome patients: a comparative study.
[So] Source:J Clin Pediatr Dent;37(3):289-95, 2013.
[Is] ISSN:1053-4628
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: The aim of this study was to characterize the main periodontal bacterial species in Down syndrome (DS) patients with and without periodontitis. METHOD: This cross-sectional study involved 75 DS patients, 45 with and 30 without periodontitis. Informed consent, health and dental questionnaires and periodontitis diagnosis were performed PCR and LAMP assays were performed on subgingival dental plaque sample. RESULTS: Tannerella forsythia was the most frequent bacteria detected in the group with and without periodontitis (95.5 and 63.3%) followed by Treponema denticola (88.8 and 50%) and Porphyromonas gingivalis (53.3 and 25% respectively). There were statistical differences between groups (p < 0.05). Pg fimA type I was the most frequent Porphyromonas gingivalis genotype. Two different sets of primers (Aa-F/Aa-R and ltx3/ltx4) were used to detect Aggregatibacter actinomycetemcomitans and different frequencies were obtained, (68% and 14.6% respectively), they had a weak correlation (Cohen Kappa = 0.16). After sequencing of PCR products, ltx3/ltx4 showed more specificity. JP2 clone of A. actinomycetemcomitans was not detected in any sample. CONCLUSIONS: The composition of oral biofilm is fundamental for the development of periodontal disease independently of immunological alterations associated with DS. The frequency of detection of A. actinomycetemcomitans reported in the literature has a wide range, because the primers and probes applied
[Mh] Termos MeSH primário: Biofilmes/classificação
Placa Dentária/microbiologia
Síndrome de Down/microbiologia
Periodontite/microbiologia
[Mh] Termos MeSH secundário: Aggregatibacter actinomycetemcomitans/classificação
Aggregatibacter actinomycetemcomitans/genética
Aggregatibacter actinomycetemcomitans/isolamento & purificação
Toxinas Bacterianas/genética
Bacteroides/isolamento & purificação
Estudos Transversais
Primers do DNA
DNA Bacteriano/análise
Exotoxinas/genética
Feminino
Proteínas de Fímbrias/análise
Genótipo
Seres Humanos
Masculino
Consórcios Microbianos
Perda da Inserção Periodontal/classificação
Perda da Inserção Periodontal/microbiologia
Bolsa Periodontal/classificação
Bolsa Periodontal/microbiologia
Periodontite/classificação
Periodonto/microbiologia
Pili Sexual/genética
Porphyromonas gingivalis/genética
Porphyromonas gingivalis/isolamento & purificação
Perda de Dente/classificação
Treponema denticola/isolamento & purificação
Adulto Jovem
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Toxins); 0 (DNA Primers); 0 (DNA, Bacterial); 0 (Exotoxins); 0 (fimbrillin); 0 (leukotoxin); 147680-16-8 (Fimbriae Proteins)
[Em] Mês de entrada:1308
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:130717
[St] Status:MEDLINE


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[PMID]:23809984
[Au] Autor:Nagano K; Abiko Y; Yoshida Y; Yoshimura F
[Ad] Endereço:Department of Microbiology, School of Dentistry, Aichi Gakuin University, Nagoya, Japan. nagano@dpc.agu.ac.jp
[Ti] Título:Genetic and antigenic analyses of Porphyromonas gingivalis FimA fimbriae.
[So] Source:Mol Oral Microbiol;28(5):392-403, 2013 Oct.
[Is] ISSN:2041-1014
[Cp] País de publicação:Denmark
[La] Idioma:eng
[Ab] Resumo:The periodontal pathogen Porphyromonas gingivalis generally expresses two distinct fimbriae, FimA and Mfa1, which play a role in biofilm formation. The fimA gene that encodes FimA fimbrilin is polymorphic, and polymerase chain reaction analysis has identified six genotypes called types I-V and Ib. We found recently that fimbriae exhibit antigenic heterogeneity among the genotypes. In the present study, we analysed the fimA DNA sequences of 84 strains of P. gingivalis and characterized the antigenicity of FimA fimbriae. Strains analysed here comprised 10, 16, 29, 13, 10 and 6 strains of types I, Ib, II, III, IV and V, respectively. DNA sequencing revealed that type Ib does not represent a single cluster and that type II sequences are remarkably diverse. In contrast, the fimA sequences of the other types were relatively homogeneous. Antigenicity was investigated using antisera elicited by pure FimA fimbriae of types I-V. Antigenicity correlated generally with the respective genotype. Type Ib strains were recognized by type I antisera. However, some strains showed cross-reactivity, especially, many type II strains reacted with type III antisera. The levels of fimbrial expression were highly variable, and expression was positively correlated with ability of biofilm formation on a saliva-coated plate. Further, two strains without FimA and Mfa1 fimbriae expressed fimbrial structures, suggesting that the strains produce other types of fimbriae.
[Mh] Termos MeSH primário: Antígenos de Bactérias/genética
Proteínas de Fímbrias/genética
Pili Sexual/genética
Porphyromonas gingivalis/genética
[Mh] Termos MeSH secundário: Variação Antigênica/genética
Antígenos de Bactérias/classificação
Antígenos de Bactérias/imunologia
Proteínas de Bactérias/genética
Proteínas de Bactérias/imunologia
Biofilmes
Reações Cruzadas/imunologia
DNA Bacteriano/genética
Película Dentária/microbiologia
Proteínas de Fímbrias/classificação
Proteínas de Fímbrias/imunologia
Genótipo
Seres Humanos
Fases de Leitura Aberta/genética
Pili Sexual/imunologia
Porphyromonas gingivalis/imunologia
Saliva/microbiologia
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antigens, Bacterial); 0 (Bacterial Proteins); 0 (DNA, Bacterial); 0 (Mfa1 protein, Porphyromonas gingivalis); 0 (fimbrillin); 147680-16-8 (Fimbriae Proteins)
[Em] Mês de entrada:1504
[Cu] Atualização por classe:130823
[Lr] Data última revisão:
130823
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:130702
[St] Status:MEDLINE
[do] DOI:10.1111/omi.12032


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[PMID]:23721858
[Au] Autor:Dziewit L; Grzesiak J; Ciok A; Nieckarz M; Zdanowski MK; Bartosik D
[Ad] Endereço:University of Warsaw, Faculty of Biology, Institute of Microbiology, Department of Bacterial Genetics, Miecznikowa 1, 02-096 Warsaw, Poland. ldziewit@biol.uw.edu.pl
[Ti] Título:Sequence determination and analysis of three plasmids of Pseudomonas sp. GLE121, a psychrophile isolated from surface ice of Ecology Glacier (Antarctica).
[So] Source:Plasmid;70(2):254-62, 2013 Sep.
[Is] ISSN:1095-9890
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pseudomonas sp. GLE121 (a psychrophilic Antarctic strain) carries three plasmids: pGLE121P1 (6899 bp), pGLE121P2 (8330 bp) and pGLE121P3 (39,583 bp). Plasmids pGLE121P1 and pGLE121P2 show significant sequence similarity to members of the IncP-9 and IncP-7 incompatibility groups, respectively, while the largest replicon, pGLE121P3, is highly related to plasmid pNCPPB880-40 of Pseudomonas syringae pathovar tomato NCPPB880. All three plasmids have a narrow host range, limited to members of the genus Pseudomonas. Plasmid pGLE121P3 encodes a conjugal transfer system, while pGLE121P1 carries only a putative MOB module, conserved in many mobilizable plasmids. Plasmid pGLE121P3 contains an additional load of genetic information, including a pair of genes with homology to the rulAB operon, responsible for ultraviolet radiation (UVR) tolerance. Given the increasing UV exposure in Antarctic regions, the expression of these genes is likely to be an important adaptive response.
[Mh] Termos MeSH primário: Camada de Gelo/microbiologia
Plasmídeos/genética
Pseudomonas/genética
[Mh] Termos MeSH secundário: Regiões Antárticas
Sequência de Bases
Biologia Computacional
Testes de Sensibilidade a Antimicrobianos por Disco-Difusão
Transferência Genética Horizontal/genética
Dados de Sequência Molecular
Pili Sexual/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1402
[Cu] Atualização por classe:130806
[Lr] Data última revisão:
130806
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130601
[St] Status:MEDLINE



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