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  1 / 10752 MEDLINE  
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[PMID]:28461121
[Au] Autor:Hall JPJ; Brockhurst MA; Dytham C; Harrison E
[Ad] Endereço:Department of Animal and Plant Sciences, University of Sheffield, Sheffield S10 2TN, UK.
[Ti] Título:The evolution of plasmid stability: Are infectious transmission and compensatory evolution competing evolutionary trajectories?
[So] Source:Plasmid;91:90-95, 2017 May.
[Is] ISSN:1095-9890
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Conjugative plasmids are widespread and play an important role in bacterial evolution by accelerating adaptation through horizontal gene transfer. However, explaining the long-term stability of plasmids remains challenging because segregational loss and the costs of plasmid carriage should drive the loss of plasmids though purifying selection. Theoretical and experimental studies suggest two key evolutionary routes to plasmid stability: First, the evolution of high conjugation rates would allow plasmids to survive through horizontal transmission as infectious agents, and second, compensatory evolution to ameliorate the cost of plasmid carriage can weaken purifying selection against plasmids. How these two evolutionary strategies for plasmid stability interact is unclear. Here, we summarise the literature on the evolution of plasmid stability and then use individual based modelling to investigate the evolutionary interplay between the evolution of plasmid conjugation rate and cost amelioration. We find that, individually, both strategies promote plasmid stability, and that they act together to increase the likelihood of plasmid survival. However, due to the inherent costs of increasing conjugation rate, particularly where conjugation is unlikely to be successful, our model predicts that amelioration is the more likely long-term solution to evolving stable bacteria-plasmid associations. Our model therefore suggests that bacteria-plasmid relationships should evolve towards lower plasmid costs that may forestall the evolution of highly conjugative, 'infectious' plasmids.
[Mh] Termos MeSH primário: Bactérias/genética
Conjugação Genética
Regulação Bacteriana da Expressão Gênica
Transferência Genética Horizontal
Modelos Estatísticos
Plasmídeos/química
[Mh] Termos MeSH secundário: Bactérias/metabolismo
Evolução Biológica
Cromossomos Bacterianos/química
Cromossomos Bacterianos/metabolismo
Aptidão Genética
Loci Gênicos
Mutagênese Insercional
Plasmídeos/metabolismo
Seleção Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE


  2 / 10752 MEDLINE  
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[PMID]:29346402
[Au] Autor:Schabort DTWP; Kilian SG; du Preez JC
[Ad] Endereço:Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, Bloemfontein, South Africa.
[Ti] Título:Gene regulation in Kluyveromyces marxianus in the context of chromosomes.
[So] Source:PLoS One;13(1):e0190913, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Eukaryotes, including the unicellular eukaryotes such as yeasts, employ multiple levels of gene regulation. Regulation of chromatin structure through chromatin compaction cascades, and influenced by transcriptional insulators, might play a role in the coordinated regulation of genes situated at adjacent loci and expressed as a co-regulated cluster. Subtelomeric gene silencing, which has previously been described in the yeast Saccharomyces cerevisiae, is an example of this phenomenon. Transcription from a common regulatory element located around a shared intergenic region is another factor that could coordinate the transcription of genes at adjacent loci. Additionally, the presence of DNA binding sites for the same transcription factor may coordinate expression of multiple genes. Yeasts such as the industrially important Kluyveromyces marxianus may also display these modes of regulation, but this has not been explored to date. An exploration was done using a complete genome and RNA-seq data from a previous study of the transcriptional response to glucose or xylose as the carbon source in a defined culture medium, and investigating whether the species displays clusters of co-localised differentially expressed genes. Regions of possible subtelomeric silencing were evident, but were non-responsive to the carbon sources tested here. Additionally, glucose or xylose responsive clusters were discovered far from telomeres which contained some of the most significantly differentially expressed genes, encoding enzymes involved in the utilisation of alternative carbon sources such as the industrially important inulinase gene INU1. These clusters contained putative binding sites for the carbon source responsive transcription factors Mig1 and Adr1. Additionally, we investigated the potential contribution of common intergenic regions in co-regulation. Some observations were also made in terms of the evolutionary conservation of these clusters among yeast species and the presence of potential transcriptional insulators at the periphery of these clusters.
[Mh] Termos MeSH primário: Cromossomos Bacterianos
Regulação Bacteriana da Expressão Gênica
Kluyveromyces/genética
[Mh] Termos MeSH secundário: Evolução Molecular
Genes Bacterianos
Glicosídeo Hidrolases/genética
Família Multigênica
Transcrição Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
EC 3.2.1.- (Glycoside Hydrolases); EC 3.2.1.7 (inulinase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180119
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190913


  3 / 10752 MEDLINE  
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[PMID]:29267353
[Au] Autor:Khan SR; Kuzminov A
[Ad] Endereço:Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America.
[Ti] Título:Degradation of RNA during lysis of Escherichia coli cells in agarose plugs breaks the chromosome.
[So] Source:PLoS One;12(12):e0190177, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The nucleoid of Escherichia coli comprises DNA, nucleoid associated proteins (NAPs) and RNA, whose role is unclear. We found that lysing bacterial cells embedded in agarose plugs in the presence of RNases caused massive fragmentation of the chromosomal DNA. This RNase-induced chromosomal fragmentation (RiCF) was completely dependent on the presence of RNase around lysing cells, while the maximal chromosomal breakage required fast cell lysis. Cell lysis in plugs without RNAse made the chromosomal DNA resistant to subsequent RNAse treatment. RiCF was not influenced by changes in the DNA supercoiling, but was influenced by growth temperature or age of the culture. RiCF was partially dependent on H-NS, histone-like nucleoid structuring- and global transcription regulator protein. The hupAB deletion of heat-unstable nucleoid protein (HU) caused increase in spontaneous fragmentation that was further increased when combined with deletions in two non-coding RNAs, nc1 and nc5. RiCF was completely dependent upon endonuclease I, a periplasmic deoxyribonuclease that is normally found inhibited by cellular RNA. Unlike RiCF, the spontaneous fragmentation in hupAB nc1 nc5 quadruple mutant was resistant to deletion of endonuclease I. RiCF-like phenomenon was observed without addition of RNase to agarose plugs if EDTA was significantly reduced during cell lysis. Addition of RNase under this condition was synergistic, breaking chromosomes into pieces too small to be retained by the pulsed field gels. RNase-independent fragmentation was qualitatively and quantitatively comparable to RiCF and was partially mediated by endonuclease I.
[Mh] Termos MeSH primário: Cromossomos Bacterianos
Escherichia coli/genética
RNA/metabolismo
Sefarose/química
[Mh] Termos MeSH secundário: Escherichia coli/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
63231-63-0 (RNA); 9012-36-6 (Sepharose)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190177


  4 / 10752 MEDLINE  
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[PMID]:29253888
[Au] Autor:Norris MH; Heacock-Kang Y; Zarzycki-Siek J; Bluhm AP; McMillan IA; Schweizer HP; Hoang TT
[Ad] Endereço:Department of Microbiology, University of Hawaii at Manoa, Honolulu, HI, United States of America.
[Ti] Título:Burkholderia pseudomallei natural competency and DNA catabolism: Identification and characterization of relevant genes from a constructed fosmid library.
[So] Source:PLoS One;12(12):e0189018, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Burkholderia spp. are genetically and physiologically diverse. Some strains are naturally transformable and capable of DNA catabolism. Burkholderia pseudomallei (Bp) strains 1026b and K96243 and B. thailandensis strain E264 are able to utilize DNA as a sole carbon source for growth, while only strains 1026b and E264 are naturally transformable. In this study, we constructed low-copy broad-host-range fosmid library, containing Bp strain 1026b chromosomal DNA fragments, and employed a novel positive selection approach to identify genes responsible for DNA uptake and DNA catabolism. The library was transferred to non-competent Bp K96243 and B. cenocepacia (Bc) K56-2, harboring chromosomally-inserted FRT-flanked sacB and pheS counter-selection markers. The library was incubated with DNA encoding Flp recombinase, followed by counter-selection on sucrose and chlorinated phenylalanine, to select for clones that took up flp-DNA, transiently expressed Flp, and excised the sacB-pheS cassette. Putative clones that survived the counter-selection were subsequently incubated with gfp-DNA and bacteria were visualized via fluorescent microscopy to confirm natural competency. Fosmid sequencing identified several 1026b genes implicated in DNA uptake, which were validated using chromosomal mutants. One of the naturally competent clones selected in Bc K56-2 enabled Bc, Bp and B. mallei to utilize DNA as a sole carbon source, and all fosmids were used to successfully create mutations in non-naturally-competent B. mallei and Bp strains.
[Mh] Termos MeSH primário: Burkholderia pseudomallei/genética
DNA Bacteriano/metabolismo
Biblioteca Gênica
Genes Bacterianos
Plasmídeos/genética
[Mh] Termos MeSH secundário: Alelos
Cromossomos Bacterianos/genética
Células Clonais
Reprodutibilidade dos Testes
Especificidade da Espécie
Transformação Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189018


  5 / 10752 MEDLINE  
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[PMID]:29065183
[Au] Autor:Wei Y; Deng P; Mohsin A; Yang Y; Zhou H; Guo M; Fang H
[Ad] Endereço:State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
[Ti] Título:An electroporation-free method based on Red recombineering for markerless deletion and genomic replacement in the Escherichia coli DH1 genome.
[So] Source:PLoS One;12(10):e0186891, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The λ-Red recombination system is a popular method for gene editing. However, its applications are limited due to restricted electroporation of DNA fragments. Here, we present an electroporation-free λ-Red recombination method in which target DNA fragments are excised by I-CreI endonuclease in vivo from the landing pad plasmid. Subsequently, the I-SceI endonuclease-cutting chromosome and DNA double-strand break repair were required. Markerless deletion and genomic replacement were successfully accomplished by this novel approach. Eight nonessential regions of 2.4-104.4 kb in the Escherichia coli DH1 genome were deleted separately with selection efficiencies of 5.3-100%. Additionally, the recombination efficiencies were 2.5-45%, representing an order of magnitude improvement over the electroporation method. For example, for genomic replacement, lycopene expression flux (3.5 kb) was efficiently and precisely integrated into the chromosome, accompanied by replacement of nonessential regions separately into four differently oriented loci. The lycopene production level varied approximately by 5- and 10-fold, corresponding to the integrated position and expression direction, respectively, in the E. coli chromosome.
[Mh] Termos MeSH primário: Eletroporação
Escherichia coli/genética
Genoma Bacteriano
Recombinação Genética
[Mh] Termos MeSH secundário: Carotenoides/metabolismo
Cromossomos Bacterianos
Plasmídeos
Deleção de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
36-88-4 (Carotenoids); SB0N2N0WV6 (lycopene)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171025
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186891


  6 / 10752 MEDLINE  
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[PMID]:28968392
[Au] Autor:Sinha AK; Durand A; Desfontaines JM; Iurchenko I; Auger H; Leach DRF; Barre FX; Michel B
[Ad] Endereço:Bacterial DNA stability, Genome biology department, Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, France.
[Ti] Título:Division-induced DNA double strand breaks in the chromosome terminus region of Escherichia coli lacking RecBCD DNA repair enzyme.
[So] Source:PLoS Genet;13(10):e1006895, 2017 Oct.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Marker frequency analysis of the Escherichia coli recB mutant chromosome has revealed a deficit of DNA in a specific zone of the terminus, centred on the dif/TerC region. Using fluorescence microscopy of a marked chromosomal site, we show that the dif region is lost after replication completion, at the time of cell division, in one daughter cell only, and that the phenomenon is transmitted to progeny. Analysis by marker frequency and microscopy shows that the position of DNA loss is not defined by the replication fork merging point since it still occurs in the dif/TerC region when the replication fork trap is displaced in strains harbouring ectopic Ter sites. Terminus DNA loss in the recB mutant is also independent of dimer resolution by XerCD at dif and of Topo IV action close to dif. It occurs in the terminus region, at the point of inversion of the GC skew, which is also the point of convergence of specific sequence motifs like KOPS and Chi sites, regardless of whether the convergence of GC skew is at dif (wild-type) or a newly created sequence. In the absence of FtsK-driven DNA translocation, terminus DNA loss is less precisely targeted to the KOPS convergence sequence, but occurs at a similar frequency and follows the same pattern as in FtsK+ cells. Importantly, using ftsIts, ftsAts division mutants and cephalexin treated cells, we show that DNA loss of the dif region in the recB mutant is decreased by the inactivation of cell division. We propose that it results from septum-induced chromosome breakage, and largely contributes to the low viability of the recB mutant.
[Mh] Termos MeSH primário: Cromossomos Bacterianos/genética
Quebras de DNA de Cadeia Dupla
Proteínas de Escherichia coli/genética
Escherichia coli/genética
Exodesoxirribonuclease V/genética
[Mh] Termos MeSH secundário: Divisão Celular
Reparo do DNA
Replicação do DNA
DNA Bacteriano/genética
Proteínas de Escherichia coli/metabolismo
Exodesoxirribonuclease V/metabolismo
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Escherichia coli Proteins); EC 3.1.11.5 (Exodeoxyribonuclease V); EC 3.1.11.5 (exodeoxyribonuclease V, E coli)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171003
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1006895


  7 / 10752 MEDLINE  
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[PMID]:28945748
[Au] Autor:Pilla G; McVicker G; Tang CM
[Ad] Endereço:Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom.
[Ti] Título:Genetic plasticity of the Shigella virulence plasmid is mediated by intra- and inter-molecular events between insertion sequences.
[So] Source:PLoS Genet;13(9):e1007014, 2017 Sep.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Acquisition of a single copy, large virulence plasmid, pINV, led to the emergence of Shigella spp. from Escherichia coli. The plasmid encodes a Type III secretion system (T3SS) on a 30 kb pathogenicity island (PAI), and is maintained in a bacterial population through a series of toxin:antitoxin (TA) systems which mediate post-segregational killing (PSK). The T3SS imposes a significant cost on the bacterium, and strains which have lost the plasmid and/or genes encoding the T3SS grow faster than wild-type strains in the laboratory, and fail to bind the indicator dye Congo Red (CR). Our aim was to define the molecular events in Shigella flexneri that cause loss of Type III secretion (T3S), and to examine whether TA systems exert positional effects on pINV. During growth at 37°C, we found that deletions of regions of the plasmid including the PAI lead to the emergence of CR-negative colonies; deletions occur through intra-molecular recombination events between insertion sequences (ISs) flanking the PAI. Furthermore, by repositioning MvpAT (which belongs to the VapBC family of TA systems) near the PAI, we demonstrate that the location of this TA system alters the rearrangements that lead to loss of T3S, indicating that MvpAT acts both globally (by reducing loss of pINV through PSK) as well as locally (by preventing loss of adjacent sequences). During growth at environmental temperatures, we show for the first time that pINV spontaneously integrates into different sites in the chromosome, and this is mediated by inter-molecular events involving IS1294. Integration leads to reduced PAI gene expression and impaired secretion through the T3SS, while excision of pINV from the chromosome restores T3SS function. Therefore, pINV integration provides a reversible mechanism for Shigella to circumvent the metabolic burden imposed by pINV. Intra- and inter-molecular events between ISs, which are abundant in Shigella spp., mediate plasticity of S. flexneri pINV.
[Mh] Termos MeSH primário: Plasmídeos/genética
Shigella/genética
Sistemas de Secreção Tipo III/genética
[Mh] Termos MeSH secundário: Cromossomos Bacterianos/genética
Escherichia coli/genética
Regulação Bacteriana da Expressão Gênica
Ilhas Genômicas/genética
Shigella/patogenicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Type III Secretion Systems)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171022
[Lr] Data última revisão:
171022
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170926
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1007014


  8 / 10752 MEDLINE  
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[PMID]:28938018
[Au] Autor:Cole BJ; Feltcher ME; Waters RJ; Wetmore KM; Mucyn TS; Ryan EM; Wang G; Ul-Hasan S; McDonald M; Yoshikuni Y; Malmstrom RR; Deutschbauer AM; Dangl JL; Visel A
[Ad] Endereço:US Department of Energy Joint Genome Institute, Walnut Creek, California, United States of America.
[Ti] Título:Genome-wide identification of bacterial plant colonization genes.
[So] Source:PLoS Biol;15(9):e2002860, 2017 Sep.
[Is] ISSN:1545-7885
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Diverse soil-resident bacteria can contribute to plant growth and health, but the molecular mechanisms enabling them to effectively colonize their plant hosts remain poorly understood. We used randomly barcoded transposon mutagenesis sequencing (RB-TnSeq) in Pseudomonas simiae, a model root-colonizing bacterium, to establish a genome-wide map of bacterial genes required for colonization of the Arabidopsis thaliana root system. We identified 115 genes (2% of all P. simiae genes) with functions that are required for maximal competitive colonization of the root system. Among the genes we identified were some with obvious colonization-related roles in motility and carbon metabolism, as well as 44 other genes that had no or vague functional predictions. Independent validation assays of individual genes confirmed colonization functions for 20 of 22 (91%) cases tested. To further characterize genes identified by our screen, we compared the functional contributions of P. simiae genes to growth in 90 distinct in vitro conditions by RB-TnSeq, highlighting specific metabolic functions associated with root colonization genes. Our analysis of bacterial genes by sequence-driven saturation mutagenesis revealed a genome-wide map of the genetic determinants of plant root colonization and offers a starting point for targeted improvement of the colonization capabilities of plant-beneficial microbes.
[Mh] Termos MeSH primário: Arabidopsis/microbiologia
Genes Bacterianos
Pseudomonas/genética
[Mh] Termos MeSH secundário: Mapeamento Cromossômico
Cromossomos Bacterianos
Código de Barras de DNA Taxonômico
Elementos de DNA Transponíveis
DNA Bacteriano
Mutação
Raízes de Plantas/microbiologia
Pseudomonas/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Transposable Elements); 0 (DNA, Bacterial)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171022
[Lr] Data última revisão:
171022
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170923
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pbio.2002860


  9 / 10752 MEDLINE  
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[PMID]:28911105
[Au] Autor:Taylor JA; Panis G; Viollier PH; Marczynski GT
[Ad] Endereço:Department of Microbiology and Immunology, McGill University, 3775 University St., Montreal, QC H3A 2B4, Canada.
[Ti] Título:A novel nucleoid-associated protein coordinates chromosome replication and chromosome partition.
[So] Source:Nucleic Acids Res;45(15):8916-8929, 2017 Sep 06.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We searched for regulators of chromosome replication in the cell cycle model Caulobacter crescentus and found a novel DNA-binding protein (GapR) that selectively aids the initiation of chromosome replication and the initial steps of chromosome partitioning. The protein binds the chromosome origin of replication (Cori) and has higher-affinity binding to mutated Cori-DNA that increases Cori-plasmid replication in vivo. gapR gene expression is essential for normal rapid growth and sufficient GapR levels are required for the correct timing of chromosome replication. Whole genome ChIP-seq identified dynamic DNA-binding distributions for GapR, with the strongest associations at the partitioning (parABS) locus near Cori. Using molecular-genetic and fluorescence microscopy experiments, we showed that GapR also promotes the first steps of chromosome partitioning, the initial separation of the duplicated parS loci following replication from Cori. This separation occurs before the parABS-dependent partitioning phase. Therefore, this early separation, whose mechanisms is not known, coincides with the poorly defined mechanism(s) that establishes chromosome asymmetry: C. crescentus chromosomes are partitioned to distinct cell-poles which develop into replicating and non-replicating cell-types. We propose that GapR coordinates chromosome replication with asymmetry-establishing chromosome separation, noting that both roles are consistent with the phylogenetic restriction of GapR to asymmetrically dividing bacteria.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Caulobacter crescentus/genética
Segregação de Cromossomos
Cromossomos Bacterianos/metabolismo
Replicação do DNA
Proteínas de Ligação a DNA/genética
[Mh] Termos MeSH secundário: Proteínas de Bactérias/metabolismo
Caulobacter crescentus/efeitos dos fármacos
Caulobacter crescentus/metabolismo
Divisão Celular/efeitos dos fármacos
Cromossomos Bacterianos/ultraestrutura
Proteínas de Ligação a DNA/metabolismo
Regulação Bacteriana da Expressão Gênica
Mutação
Novobiocina/farmacologia
Plasmídeos/química
Plasmídeos/metabolismo
Origem de Replicação
Rifampina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (DNA-Binding Proteins); 17EC19951N (Novobiocin); VJT6J7R4TR (Rifampin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170916
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx596


  10 / 10752 MEDLINE  
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[PMID]:28911099
[Au] Autor:Le S; Serrano E; Kawamura R; Carrasco B; Yan J; Alonso JC
[Ad] Endereço:Department of Physics, National University of Singapore, 117551, Singapore.
[Ti] Título:Bacillus subtilis RecA with DprA-SsbA antagonizes RecX function during natural transformation.
[So] Source:Nucleic Acids Res;45(15):8873-8885, 2017 Sep 06.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bacillus subtilis DprA and RecX proteins, which interact with RecA, are crucial for efficient chromosomal and plasmid transformation. We showed that RecA, in the rATP·Mg2+ bound form (RecA·ATP), could not compete with RecX, SsbA or SsbB for assembly onto single-stranded (ss)DNA, but RecA·dATP partially displaced these proteins from ssDNA. RecX promoted reversible depolymerization of preformed RecA·ATP filaments. The two-component DprA-SsbA mediator reversed the RecX negative effect on RecA filament extension, but not DprA or DprA and SsbB. In the presence of DprA-SsbA, RecX added prior to RecA·ATP inhibited DNA strand exchange, but this inhibition was reversed when RecX was added after RecA. We propose that RecA nucleation is more sensitive to RecX action than is RecA filament growth. DprA-SsbA facilitates formation of an active RecA filament that directly antagonizes the inhibitory effects of RecX. RecX and DprA enable chromosomal transformation by altering RecA filament dynamics. DprA-SsbA and RecX proteins constitute a new regulatory network of RecA function. DprA-SsbA contributes to the formation of an active RecA filament and directly antagonizes the inhibitory effects of RecX during natural transformation.
[Mh] Termos MeSH primário: Bacillus subtilis/genética
Proteínas de Bactérias/genética
Proteínas de Ligação a DNA/genética
Regulação Bacteriana da Expressão Gênica
Proteínas de Membrana/genética
Recombinases Rec A/genética
Transformação Bacteriana
[Mh] Termos MeSH secundário: Trifosfato de Adenosina/metabolismo
Bacillus subtilis/metabolismo
Proteínas de Bactérias/metabolismo
Cromossomos Bacterianos/química
Cromossomos Bacterianos/metabolismo
DNA Bacteriano/genética
DNA Bacteriano/metabolismo
DNA de Cadeia Simples/genética
DNA de Cadeia Simples/metabolismo
Proteínas de Ligação a DNA/metabolismo
Cinética
Proteínas de Membrana/metabolismo
Plasmídeos/química
Plasmídeos/metabolismo
Recombinases Rec A/metabolismo
Recombinação Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (DNA, Bacterial); 0 (DNA, Single-Stranded); 0 (DNA-Binding Proteins); 0 (DprA protein, bacteria); 0 (Membrane Proteins); 0 (RecX protein, Xanthomonas campestris); 8L70Q75FXE (Adenosine Triphosphate); EC 2.7.7.- (Rec A Recombinases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170916
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx583



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