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  1 / 4396 MEDLINE  
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[PMID]:29298360
[Au] Autor:Habib AGK; Sugiura K; Ueno M
[Ad] Endereço:Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Kagamiyama, Higashi-Hiroshima, Japan.
[Ti] Título:Chromosome passenger complex is required for the survival of cells with ring chromosomes in fission yeast.
[So] Source:PLoS One;13(1):e0190523, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ring chromosomes are circular chromosomal abnormalities that have been reported in association with some genetic disorders and cancers. In Schizosaccharomyces pombe, lack of function of protection of telomere 1 (Pot1) or telomerase catalytic subunit (Trt1) results in survivors with circular chromosomes. Hitherto, it is poorly understood how cells with circular chromosomes survive and how circular chromosomes are maintained. Fission yeast Cut17/Bir1, Ark1, Pic1, and Nbl1 is a conserved chromosome passenger complex (CPC) functioning mainly throughout mitosis. Here, using a temperature-sensitive mutant of CPC subunits, we determined that CPC is synthetically lethal in combination with either Pot1 or Trt1. The pot1Δ pic1-T269 double mutant, which has circular chromosomes, showed a high percentage of chromosome mis-segregation and DNA damage foci at 33°C. We furthermore found that neither Shugoshin Sgo2 nor heterochromatin protein Swi6, which contribute to the centromeric localization of CPC, were required for the survival in the absence of Pot1. Both the pot1Δ sgo2Δ and pot1Δ swi6Δ double mutants displayed a high percentage of DNA damage foci, but a low percentage of chromosome mis-segregation, suggesting the link between the high percentage of chromosome mis-segregation and the lethality of the CPC pot1Δ double mutant. Our results suggest that CPC is required for the survival of cells with circular chromosomes and sheds light on the possible roles of CPC in the maintenance of circular chromosomes.
[Mh] Termos MeSH primário: Cromossomos Fúngicos
Cromossomos em Anel
Schizosaccharomyces/genética
[Mh] Termos MeSH secundário: Sobrevivência Celular
Dano ao DNA
DNA Fúngico/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Fungal)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180104
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190523


  2 / 4396 MEDLINE  
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[PMID]:29208645
[Au] Autor:Mariezcurrena A; Uhlmann F
[Ad] Endereço:Chromosome Segregation Laboratory, The Francis Crick Institute, London NW1 1AT, United Kingdom.
[Ti] Título:Observation of DNA intertwining along authentic budding yeast chromosomes.
[So] Source:Genes Dev;31(21):2151-2161, 2017 11 01.
[Is] ISSN:1549-5477
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:DNA replication of circular genomes generates physically interlinked or catenated sister DNAs. These are resolved through transient DNA fracture by type II topoisomerases to permit chromosome segregation during cell division. Topoisomerase II is similarly required for linear chromosome segregation, suggesting that linear chromosomes also remain intertwined following DNA replication. Indeed, chromosome resolution defects are a frequent cause of chromosome segregation failure and consequent aneuploidies. When and where intertwines arise and persist along linear chromosomes are not known, owing to the difficulty of demonstrating intertwining of linear DNAs. Here, we used excision of chromosomal regions as circular "loop outs" to convert sister chromatid intertwines into catenated circles. This revealed intertwining at replication termination and cohesin-binding sites, where intertwines are thought to arise and persist but not to a greater extent than elsewhere in the genome. Intertwining appears to spread evenly along chromosomes but is excluded from heterochromatin. We found that intertwines arise before replication termination, suggesting that replication forks rotate during replication elongation to dissipate torsion ahead of the forks. Our approach provides previously inaccessible insight into the topology of eukaryotic chromosomes and illuminates a process critical for successful chromosome segregation.
[Mh] Termos MeSH primário: Cromossomos Fúngicos/metabolismo
Replicação do DNA
DNA Fúngico/metabolismo
Saccharomyces cerevisiae/genética
Saccharomyces cerevisiae/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Ciclo Celular/metabolismo
Proteínas Cromossômicas não Histona/metabolismo
Segregação de Cromossomos
Estruturas Genéticas
Genoma Fúngico
Heterocromatina/metabolismo
Origem de Replicação/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cell Cycle Proteins); 0 (Chromosomal Proteins, Non-Histone); 0 (DNA, Fungal); 0 (Heterochromatin); 0 (cohesins)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180130
[Lr] Data última revisão:
180130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171207
[St] Status:MEDLINE
[do] DOI:10.1101/gad.305557.117


  3 / 4396 MEDLINE  
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[PMID]:28746375
[Au] Autor:Markowitz TE; Suarez D; Blitzblau HG; Patel NJ; Markhard AL; MacQueen AJ; Hochwagen A
[Ad] Endereço:Department of Biology; New York University; New York, NY; United States of America.
[Ti] Título:Reduced dosage of the chromosome axis factor Red1 selectively disrupts the meiotic recombination checkpoint in Saccharomyces cerevisiae.
[So] Source:PLoS Genet;13(7):e1006928, 2017 Jul.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Meiotic chromosomes assemble characteristic "axial element" structures that are essential for fertility and provide the chromosomal context for meiotic recombination, synapsis and checkpoint signaling. Whether these meiotic processes are equally dependent on axial element integrity has remained unclear. Here, we investigated this question in S. cerevisiae using the putative condensin allele ycs4S. We show that the severe axial element assembly defects of this allele are explained by a linked mutation in the promoter of the major axial element gene RED1 that reduces Red1 protein levels to 20-25% of wild type. Intriguingly, the Red1 levels of ycs4S mutants support meiotic processes linked to axis integrity, including DNA double-strand break formation and deposition of the synapsis protein Zip1, at levels that permit 70% gamete survival. By contrast, the ability to elicit a meiotic checkpoint arrest is completely eliminated. This selective loss of checkpoint function is supported by a RED1 dosage series and is associated with the loss of most of the cytologically detectable Red1 from the axial element. Our results indicate separable roles for Red1 in building the structural axis of meiotic chromosomes and mounting a sustained recombination checkpoint response.
[Mh] Termos MeSH primário: Dosagem de Genes/genética
Meiose/genética
Recombinação Genética
Proteínas de Saccharomyces cerevisiae/genética
[Mh] Termos MeSH secundário: Pareamento Cromossômico
Cromossomos Fúngicos/genética
Quebras de DNA de Cadeia Dupla
Mutação
Saccharomyces cerevisiae/genética
Esporos Fúngicos/genética
Complexo Sinaptonêmico/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RED1 protein, S cerevisiae); 0 (Saccharomyces cerevisiae Proteins)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:180105
[Lr] Data última revisão:
180105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1006928


  4 / 4396 MEDLINE  
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[PMID]:29186203
[Au] Autor:Shen D; Skibbens RV
[Ad] Endereço:Department of Biological Sciences, Lehigh University, Bethlehem, Pennsylvania, United States of America.
[Ti] Título:Chl1 DNA helicase and Scc2 function in chromosome condensation through cohesin deposition.
[So] Source:PLoS One;12(11):e0188739, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chl1 DNA helicase promotes sister chromatid cohesion and associates with both the cohesion establishment acetyltransferase Eco1/Ctf7 and the DNA polymerase processivity factor PCNA that supports Eco1/Ctf7 function. Mutation in CHL1 results in precocious sister chromatid separation and cell aneuploidy, defects that arise through reduced levels of chromatin-bound cohesins which normally tether together sister chromatids (trans tethering). Mutation of Chl1 family members (BACH1/BRIP/FANCJ and DDX11/ChlR1) also exhibit genotoxic sensitivities, consistent with a role for Chl1 in trans tethering which is required for efficient DNA repair. Chl1 promotes the recruitment of Scc2 to DNA which is required for cohesin deposition onto DNA. There is limited evidence, however, that Scc2 also directs the deposition onto DNA of condensins which promote tethering in cis (intramolecular DNA links). Here, we test the ability of Chl1 to promote cis tethering and the role of both Chl1 and Scc2 to promote condensin recruitment to DNA. The results reveal that chl1 mutant cells exhibit significant condensation defects both within the rDNA locus and genome-wide. Importantly, chl1 mutant cell condensation defects do not result from reduced chromatin binding of condensin, but instead through reduced chromatin binding of cohesin. We tested scc2-4 mutant cells and similarly found no evidence of reduced condensin recruitment to chromatin. Consistent with a role for Scc2 specifically in cohesin deposition, scc2-4 mutant cell condensation defects are irreversible. We thus term Chl1 a novel regulator of both chromatin condensation and sister chromatid cohesion through cohesin-based mechanisms. These results reveal an exciting interface between DNA structure and the highly conserved cohesin complex.
[Mh] Termos MeSH primário: Proteínas de Ciclo Celular/metabolismo
Proteínas Cromossômicas não Histona/metabolismo
Proteínas Cromossômicas não Histona/fisiologia
Cromossomos Fúngicos
Proteínas de Saccharomyces cerevisiae/fisiologia
Saccharomyces cerevisiae/genética
[Mh] Termos MeSH secundário: DNA Fúngico/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CHL1 protein, S cerevisiae); 0 (Cell Cycle Proteins); 0 (Chromosomal Proteins, Non-Histone); 0 (DNA, Fungal); 0 (SCC2 protein, S cerevisiae); 0 (Saccharomyces cerevisiae Proteins); 0 (cohesins)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180101
[Lr] Data última revisão:
180101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188739


  5 / 4396 MEDLINE  
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[PMID]:28455351
[Au] Autor:Chu DB; Gromova T; Newman TAC; Burgess SM
[Ad] Endereço:Department of Molecular and Cellular Biology, University of California, Davis, California 95616.
[Ti] Título:The Nucleoporin Nup2 Contains a Meiotic-Autonomous Region that Promotes the Dynamic Chromosome Events of Meiosis.
[So] Source:Genetics;206(3):1319-1337, 2017 07.
[Is] ISSN:1943-2631
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Meiosis is a specialized cellular program required to create haploid gametes from diploid parent cells. Homologous chromosomes pair, synapse, and recombine in a dynamic environment that accommodates gross chromosome reorganization and significant chromosome motion, which are critical for normal chromosome segregation. In , Ndj1 is a meiotic telomere-associated protein required for physically attaching telomeres to proteins embedded in the nuclear envelope. In this study, we identified additional proteins that act at the nuclear periphery from meiotic cell extracts, including Nup2, a nonessential nucleoporin with a known role in tethering interstitial chromosomal loci to the nuclear pore complex. We found that deleting affects meiotic progression and spore viability, and gives increased levels of recombination intermediates and products. We identified a previously uncharacterized 125 aa region of Nup2 that is necessary and sufficient for its meiotic function, thus behaving as a meiotic autonomous region (MAR). Nup2-MAR forms distinct foci on spread meiotic chromosomes, with a subset overlapping with Ndj1 foci. Localization of Nup2-MAR to meiotic chromosomes does not require Ndj1, nor does Ndj1 localization require Nup2, suggesting these proteins function in different pathways, and their interaction is weak or indirect. Instead, several severe synthetic phenotypes are associated with the Δ Δ double mutant, including delayed turnover of recombination joint molecules, and a failure to undergo nuclear divisions without also arresting the meiotic program. These data suggest Nup2 and Ndj1 support partially overlapping functions that promote two different levels of meiotic chromosome organization necessary to withstand a dynamic stage of the eukaryotic life cycle.
[Mh] Termos MeSH primário: Meiose
Complexo de Proteínas Formadoras de Poros Nucleares/metabolismo
Proteínas de Saccharomyces cerevisiae/metabolismo
[Mh] Termos MeSH secundário: Motivos de Aminoácidos
Proteínas de Ciclo Celular/genética
Proteínas de Ciclo Celular/metabolismo
Cromossomos Fúngicos/genética
Cromossomos Fúngicos/metabolismo
Recombinação Homóloga
Complexo de Proteínas Formadoras de Poros Nucleares/química
Complexo de Proteínas Formadoras de Poros Nucleares/genética
Domínios Proteicos
Saccharomyces cerevisiae/genética
Saccharomyces cerevisiae/metabolismo
Proteínas de Saccharomyces cerevisiae/química
Proteínas de Saccharomyces cerevisiae/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Cell Cycle Proteins); 0 (NUP2 protein, S cerevisiae); 0 (Ndj1 protein, S cerevisiae); 0 (Nuclear Pore Complex Proteins); 0 (Saccharomyces cerevisiae Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171213
[Lr] Data última revisão:
171213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE
[do] DOI:10.1534/genetics.116.194555


  6 / 4396 MEDLINE  
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[PMID]:28991264
[Au] Autor:Tanizawa H; Kim KD; Iwasaki O; Noma KI
[Ad] Endereço:The Wistar Institute, Philadelphia, Pennsylvania, USA.
[Ti] Título:Architectural alterations of the fission yeast genome during the cell cycle.
[So] Source:Nat Struct Mol Biol;24(11):965-976, 2017 Nov.
[Is] ISSN:1545-9985
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Eukaryotic genomes are highly ordered through various mechanisms, including topologically associating domain (TAD) organization. We employed an in situ Hi-C approach to follow the 3D organization of the fission yeast genome during the cell cycle. We demonstrate that during mitosis, large domains of 300 kb-1 Mb are formed by condensin. This mitotic domain organization does not suddenly dissolve, but gradually diminishes until the next mitosis. By contrast, small domains of 30-40 kb that are formed by cohesin are relatively stable across the cell cycle. Condensin and cohesin mediate long- and short-range contacts, respectively, by bridging their binding sites, thereby forming the large and small domains. These domains are inversely regulated during the cell cycle but assemble independently. Our study describes the chromosomal oscillation between the formation and decay phases of the large and small domains, and we predict that the condensin-mediated domains serve as chromosomal compaction units.
[Mh] Termos MeSH primário: Cromossomos Fúngicos/metabolismo
Cromossomos Fúngicos/ultraestrutura
Genoma Fúngico
Mitose
Schizosaccharomyces/citologia
Schizosaccharomyces/fisiologia
[Mh] Termos MeSH secundário: Adenosina Trifosfatases/metabolismo
Proteínas de Ciclo Celular/metabolismo
Proteínas Cromossômicas não Histona/metabolismo
Proteínas de Ligação a DNA/metabolismo
Proteínas Fúngicas/metabolismo
Complexos Multiproteicos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cell Cycle Proteins); 0 (Chromosomal Proteins, Non-Histone); 0 (DNA-Binding Proteins); 0 (Fungal Proteins); 0 (Multiprotein Complexes); 0 (cohesins); 0 (condensin complexes); EC 3.6.1.- (Adenosine Triphosphatases)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171121
[Lr] Data última revisão:
171121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171010
[St] Status:MEDLINE
[do] DOI:10.1038/nsmb.3482


  7 / 4396 MEDLINE  
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[PMID]:28981863
[Au] Autor:Tashiro S; Nishihara Y; Kugou K; Ohta K; Kanoh J
[Ad] Endereço:Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan.
[Ti] Título:Subtelomeres constitute a safeguard for gene expression and chromosome homeostasis.
[So] Source:Nucleic Acids Res;45(18):10333-10349, 2017 Oct 13.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The subtelomere, a telomere-adjacent chromosomal domain, contains species-specific homologous DNA sequences, in addition to various genes. However, the functions of subtelomeres, particularly subtelomeric homologous (SH) sequences, remain elusive. Here, we report the first comprehensive analyses of the cellular functions of SH sequences in the fission yeast, Schizosaccharomyces pombe. Complete removal of SH sequences from the genome revealed that they are dispensable for mitosis, meiosis and telomere length control. However, when telomeres are lost, SH sequences prevent deleterious inter-chromosomal end fusion by facilitating intra-chromosomal circularization. Surprisingly, SH-deleted cells sometimes survive telomere loss through inter-chromosomal end fusions via homologous loci such as LTRs, accompanied by centromere inactivation of either chromosome. Moreover, SH sequences function as a buffer region against the spreading of subtelomeric heterochromatin into the neighboring gene-rich regions. Furthermore, we found a nucleosome-free region at the subtelomeric border, which may be a second barrier that blocks heterochromatin spreading into the subtelomere-adjacent euchromatin. Thus, our results demonstrate multiple defense functions of subtelomeres in chromosome homeostasis and gene expression.
[Mh] Termos MeSH primário: Cromossomos Fúngicos/fisiologia
Expressão Gênica
Homeostase/genética
Proteínas de Schizosaccharomyces pombe/genética
Schizosaccharomyces/genética
Telômero/fisiologia
[Mh] Termos MeSH secundário: Centrômero/metabolismo
Instabilidade Cromossômica/genética
Regulação Fúngica da Expressão Gênica
Heterocromatina/metabolismo
Organismos Geneticamente Modificados
Deleção de Sequência
Proteínas de Ligação a Telômeros/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Heterochromatin); 0 (Schizosaccharomyces pombe Proteins); 0 (Telomere-Binding Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171006
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx780


  8 / 4396 MEDLINE  
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[PMID]:28973473
[Au] Autor:Benedetti F; Racko D; Dorier J; Burnier Y; Stasiak A
[Ad] Endereço:Center for Integrative Genomics, University of Lausanne, 1015 Lausanne, Switzerland.
[Ti] Título:Transcription-induced supercoiling explains formation of self-interacting chromatin domains in S. pombe.
[So] Source:Nucleic Acids Res;45(17):9850-9859, 2017 Sep 29.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The question of how self-interacting chromatin domains in interphase chromosomes are structured and generated dominates current discussions on eukaryotic chromosomes. Numerical simulations using standard polymer models have been helpful in testing the validity of various models of chromosome organization. Experimental contact maps can be compared with simulated contact maps and thus verify how good is the model. With increasing resolution of experimental contact maps, it became apparent though that active processes need to be introduced into models to recapitulate the experimental data. Since transcribing RNA polymerases are very strong molecular motors that induce axial rotation of transcribed DNA, we present here models that include such rotational motors. We also include into our models swivels and sites for intersegmental passages that account for action of DNA topoisomerases releasing torsional stress. Using these elements in our models, we show that transcription-induced supercoiling generated in the regions with divergent-transcription and supercoiling relaxation occurring between these regions are sufficient to explain formation of self-interacting chromatin domains in chromosomes of fission yeast (S. pombe).
[Mh] Termos MeSH primário: DNA Topoisomerases/química
DNA Fúngico/química
DNA Super-Helicoidal/química
RNA Polimerases Dirigidas por DNA/química
Schizosaccharomyces/genética
Transcrição Genética
[Mh] Termos MeSH secundário: Fenômenos Biomecânicos
Cromatina/química
Cromatina/metabolismo
Cromossomos Fúngicos/química
Cromossomos Fúngicos/metabolismo
DNA Topoisomerases/genética
DNA Topoisomerases/metabolismo
DNA Fúngico/genética
DNA Fúngico/metabolismo
DNA Super-Helicoidal/genética
DNA Super-Helicoidal/metabolismo
RNA Polimerases Dirigidas por DNA/genética
RNA Polimerases Dirigidas por DNA/metabolismo
Regulação Fúngica da Expressão Gênica
Simulação de Dinâmica Molecular
Rotação
Schizosaccharomyces/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chromatin); 0 (DNA, Fungal); 0 (DNA, Superhelical); EC 2.7.7.6 (DNA-Directed RNA Polymerases); EC 5.99.1.- (DNA Topoisomerases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171004
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx716


  9 / 4396 MEDLINE  
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[PMID]:28854733
[Au] Autor:Zhang Q; Bassetti F; Gherardi M; Lagomarsino MC
[Ad] Endereço:Sorbonne Universités, UPMC Univ Paris 06, UMR 7238, Computational and Quantitative Biology, 4 Place Jussieu, Paris, France.
[Ti] Título:Cell-to-cell variability and robustness in S-phase duration from genome replication kinetics.
[So] Source:Nucleic Acids Res;45(14):8190-8198, 2017 Aug 21.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Genome replication, a key process for a cell, relies on stochastic initiation by replication origins, causing a variability of replication timing from cell to cell. While stochastic models of eukaryotic replication are widely available, the link between the key parameters and overall replication timing has not been addressed systematically. We use a combined analytical and computational approach to calculate how positions and strength of many origins lead to a given cell-to-cell variability of total duration of the replication of a large region, a chromosome or the entire genome. Specifically, the total replication timing can be framed as an extreme-value problem, since it is due to the last region that replicates in each cell. Our calculations identify two regimes based on the spread between characteristic completion times of all inter-origin regions of a genome. For widely different completion times, timing is set by the single specific region that is typically the last to replicate in all cells. Conversely, when the completion time of all regions are comparable, an extreme-value estimate shows that the cell-to-cell variability of genome replication timing has universal properties. Comparison with available data shows that the replication program of three yeast species falls in this extreme-value regime.
[Mh] Termos MeSH primário: Algoritmos
Período de Replicação do DNA/genética
Genoma/genética
Modelos Genéticos
Origem de Replicação/genética
Fase S/genética
[Mh] Termos MeSH secundário: Cromossomos Fúngicos/genética
Biologia Computacional/métodos
Cinética
Saccharomyces cerevisiae/citologia
Saccharomyces cerevisiae/genética
Saccharomycetales/citologia
Saccharomycetales/genética
Schizosaccharomyces/citologia
Schizosaccharomyces/genética
Especificidade da Espécie
Processos Estocásticos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170901
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx556


  10 / 4396 MEDLINE  
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[PMID]:28825727
[Au] Autor:Kakui Y; Rabinowitz A; Barry DJ; Uhlmann F
[Ad] Endereço:Chromosome Segregation Laboratory, The Francis Crick Institute, London, UK.
[Ti] Título:Condensin-mediated remodeling of the mitotic chromatin landscape in fission yeast.
[So] Source:Nat Genet;49(10):1553-1557, 2017 Oct.
[Is] ISSN:1546-1718
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The eukaryotic genome consists of DNA molecules far longer than the cells that contain them. They reach their greatest compaction during chromosome condensation in mitosis. This process is aided by condensin, a structural maintenance of chromosomes (SMC) family member. The spatial organization of mitotic chromosomes and how condensin shapes chromatin architecture are not yet fully understood. Here we use chromosome conformation capture (Hi-C) to study mitotic chromosome condensation in the fission yeast Schizosaccharomyces pombe. This showed that the interphase landscape characterized by small chromatin domains is replaced by fewer but larger domains in mitosis. Condensin achieves this by setting up longer-range, intrachromosomal DNA interactions, which compact and individualize chromosomes. At the same time, local chromatin contacts are constrained by condensin, with profound implications for local chromatin function during mitosis. Our results highlight condensin as a major determinant that changes the chromatin landscape as cells prepare their genomes for cell division.
[Mh] Termos MeSH primário: Adenosina Trifosfatases/fisiologia
Montagem e Desmontagem da Cromatina/fisiologia
Cromossomos Fúngicos/ultraestrutura
Proteínas de Ligação a DNA/fisiologia
Complexos Multiproteicos/fisiologia
Proteínas de Schizosaccharomyces pombe/fisiologia
Schizosaccharomyces/genética
[Mh] Termos MeSH secundário: Adenosina Trifosfatases/genética
Sequência de Bases
Cromatina/ultraestrutura
Montagem e Desmontagem da Cromatina/genética
Imunoprecipitação da Cromatina
DNA Fúngico/genética
Proteínas de Ligação a DNA/genética
Desoxirribonucleases de Sítio Específico do Tipo II
Interfase
Mitose
Complexos Multiproteicos/genética
Schizosaccharomyces/ultraestrutura
Proteínas de Schizosaccharomyces pombe/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chromatin); 0 (DNA, Fungal); 0 (DNA-Binding Proteins); 0 (Multiprotein Complexes); 0 (Schizosaccharomyces pombe Proteins); 0 (condensin complexes); EC 3.1.21.4 (Deoxyribonucleases, Type II Site-Specific); EC 3.1.21.4 (GATC-specific type II deoxyribonucleases); EC 3.6.1.- (Adenosine Triphosphatases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170822
[St] Status:MEDLINE
[do] DOI:10.1038/ng.3938



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