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  1 / 2890 MEDLINE  
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[PMID]:29211750
[Au] Autor:Warden CH; Bettaieb A; Min E; Fisler JS; Haj FG; Stern JS
[Ad] Endereço:Departments of Pediatrics, Neurobiology Physiology and Behavior, University of California, Davis, Davis, CA, United States of America.
[Ti] Título:Chow fed UC Davis strain female Lepr fatty Zucker rats exhibit mild glucose intolerance, hypertriglyceridemia, and increased urine volume, all reduced by a Brown Norway strain chromosome 1 congenic donor region.
[So] Source:PLoS One;12(12):e0188175, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Our objective is to identify genes that influence the development of any phenotypes of type 2 diabetes (T2D) or kidney disease in obese animals. We use the reproductively isolated UC Davis fatty Zucker strain rat model in which the defective chromosome 4 leptin receptor (LeprfaSte/faSte) results in fatty obesity. We previously produced a congenic strain with the distal half of chromosome 1 from the Brown Norway strain (BN) on a Zucker (ZUC) background (BN.ZUC-D1Rat183-D1Rat90). Previously published studies in males showed that the BN congenic donor region protects from some phenotypes of renal dysfunction and T2D. We now expand our studies to include females and expand phenotyping to gene expression. We performed diabetes and kidney disease phenotyping in chow-fed females of the BN.ZUC-D1Rat183-D1Rat90 congenic strain to determine the specific characteristics of the UC Davis model. Fatty LeprfaSte/faSte animals of both BN and ZUC genotype in the congenic donor region had prediabetic levels of fasting blood glucose and blood glucose 2 hours after a glucose tolerance test. We observed significant congenic strain chromosome 1 genotype effects of the BN donor region in fatty females that resulted in decreased food intake, urine volume, glucose area under the curve during glucose tolerance test, plasma triglyceride levels, and urine glucose excretion per day. In fatty females, there were significant congenic strain BN genotype effects on non-fasted plasma urea nitrogen, triglyceride, and creatinine. Congenic region genotype effects were observed by quantitative PCR of mRNA from the kidney for six genes, all located in the chromosome 1 BN donor region, with potential effects on T2D or kidney function. The results are consistent with the hypothesis that the BN genotype chromosome 1 congenic region influences traits of both type 2 diabetes and kidney function in fatty UC Davis ZUC females and that there are many positional candidate genes.
[Mh] Termos MeSH primário: Ração Animal
Cromossomos de Mamíferos
Teste de Tolerância a Glucose
Transtornos Urinários/genética
[Mh] Termos MeSH secundário: Animais
Diabetes Mellitus Experimental/genética
Comportamento Alimentar
Feminino
Testes de Função Renal
Ratos
Ratos Zucker
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171229
[Lr] Data última revisão:
171229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171207
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188175


  2 / 2890 MEDLINE  
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[PMID]:28934466
[Au] Autor:Lu Y; Dai X; Zhang M; Miao Y; Zhou C; Cui Z; Xiong B
[Ad] Endereço:College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
[Ti] Título:Cohesin acetyltransferase Esco2 regulates SAC and kinetochore functions via maintaining H4K16 acetylation during mouse oocyte meiosis.
[So] Source:Nucleic Acids Res;45(16):9388-9397, 2017 Sep 19.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Sister chromatid cohesion, mediated by cohesin complex and established by the acetyltransferases Esco1 and Esco2, is essential for faithful chromosome segregation. Mutations in Esco2 cause Roberts syndrome, a developmental disease characterized by severe prenatal retardation as well as limb and facial abnormalities. However, its exact roles during oocyte meiosis have not clearly defined. Here, we report that Esco2 localizes to the chromosomes during oocyte meiotic maturation. Depletion of Esco2 by morpholino microinjection leads to the precocious polar body extrusion, the escape of metaphase I arrest induced by nocodazole treatment and the loss of BubR1 from kinetochores, indicative of inactivated SAC. Furthermore, depletion of Esco2 causes a severely impaired spindle assembly and chromosome alignment, accompanied by the remarkably elevated incidence of defective kinetochore-microtubule attachments which consequently lead to the generation of aneuploid eggs. Notably, we find that the involvement of Esco2 in SAC and kinetochore functions is mediated by its binding to histone H4 and acetylation of H4K16 both in vivo and in vitro. Thus, our data assign a novel meiotic function to Esco2 beyond its role in the cohesion establishment during mouse oocyte meiosis.
[Mh] Termos MeSH primário: Acetiltransferases/metabolismo
Histonas/metabolismo
Cinetocoros/metabolismo
Pontos de Checagem da Fase M do Ciclo Celular/genética
Meiose/genética
Oócitos/enzimologia
[Mh] Termos MeSH secundário: Acetilação
Acetiltransferases/fisiologia
Aneuploidia
Animais
Cromossomos de Mamíferos/enzimologia
Feminino
Histonas/química
Lisina/metabolismo
Camundongos Endogâmicos ICR
Fuso Acromático/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Histones); EC 2.3.1.- (Acetyltransferases); EC 2.3.1.- (establishment of cohesion 1 homolog 2, mouse); K3Z4F929H6 (Lysine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170922
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx563


  3 / 2890 MEDLINE  
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[PMID]:28819014
[Au] Autor:Zhang QH; Yuen WS; Adhikari D; Flegg JA; FitzHarris G; Conti M; Sicinski P; Nabti I; Marangos P; Carroll J
[Ad] Endereço:Development and Stem Cell Program, Monash Biomedicine Discovery Institute, Monash University, Melbourne, Victoria, Australia qing-hua.zhang@monash.edu.
[Ti] Título:Cyclin A2 modulates kinetochore-microtubule attachment in meiosis II.
[So] Source:J Cell Biol;216(10):3133-3143, 2017 Oct 02.
[Is] ISSN:1540-8140
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cyclin A2 is a crucial mitotic Cdk regulatory partner that coordinates entry into mitosis and is then destroyed in prometaphase within minutes of nuclear envelope breakdown. The role of cyclin A2 in female meiosis and its dynamics during the transition from meiosis I (MI) to meiosis II (MII) remain unclear. We found that cyclin A2 decreases in prometaphase I but recovers after the first meiotic division and persists, uniquely for metaphase, in MII-arrested oocytes. Conditional deletion of cyclin A2 from mouse oocytes has no discernible effect on MI but leads to disrupted MII spindles and increased merotelic attachments. On stimulation of exit from MII, there is a dramatic increase in lagging chromosomes and an inhibition of cytokinesis. These defects are associated with an increase in microtubule stability in MII spindles, suggesting that cyclin A2 mediates the fidelity of MII by maintaining microtubule dynamics during the rapid formation of the MII spindle.
[Mh] Termos MeSH primário: Cromossomos de Mamíferos/metabolismo
Ciclina A2/metabolismo
Cinetocoros/metabolismo
Meiose/fisiologia
Microtúbulos/metabolismo
[Mh] Termos MeSH secundário: Animais
Cromossomos de Mamíferos/genética
Ciclina A2/genética
Camundongos
Camundongos Knockout
Microtúbulos/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CCNA2 protein, mouse); 0 (Cyclin A2)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171007
[Lr] Data última revisão:
171007
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170819
[St] Status:MEDLINE
[do] DOI:10.1083/jcb.201607111


  4 / 2890 MEDLINE  
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[PMID]:28767705
[Au] Autor:Orsztynowicz M; Lechniak D; Pawlak P; Kociucka B; Kubickova S; Cernohorska H; Madeja ZE
[Ad] Endereço:Department of Genetics and Animal Breeding, Poznan University of Life Sciences, Poznan, Poland.
[Ti] Título:Changes in chromosome territory position within the nucleus reflect alternations in gene expression related to embryonic lineage specification.
[So] Source:PLoS One;12(8):e0182398, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Loss of totipotentcy in an early embryo is directed by molecular processes responsible for cell fate decisions. Three dimensional genome organisation is an important factor linking chromatin architecture with stage specific gene expression patterns. Little is known about the role of chromosome organisation in gene expression regulation of lineage specific factors in mammalian embryos. Using bovine embryos as a model we have described these interactions at key developmental stages. Three bovine chromosomes (BTA) that differ in size, number of carried genes, and contain loci for key lineage regulators OCT4, NANOG and CDX2, were investigated. The results suggest that large chromosomes regardless of their gene density (BTA12 gene-poor, BTA5 gene-rich) do not significantly change their radial position within the nucleus. Gene loci however, may change its position within the chromosome territory (CT) and relocate its periphery, when stage specific process of gene activation is required. Trophectoderm specific CDX2 and epiblast precursor NANOG loci tend to locate on the surface or outside of the CTs, at stages related with their high expression. We postulate that the observed changes in CT shape reflect global alternations in gene expression related to differentiation.
[Mh] Termos MeSH primário: Fator de Transcrição CDX2/genética
Núcleo Celular/genética
Cromossomos de Mamíferos/genética
Proteína Homeobox Nanog/genética
Fator 3 de Transcrição de Octâmero/genética
[Mh] Termos MeSH secundário: Animais
Fator de Transcrição CDX2/metabolismo
Bovinos
Linhagem da Célula
Desenvolvimento Embrionário
Regulação da Expressão Gênica no Desenvolvimento
Hibridização in Situ Fluorescente
Proteína Homeobox Nanog/metabolismo
Fator 3 de Transcrição de Octâmero/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CDX2 Transcription Factor); 0 (Nanog Homeobox Protein); 0 (Octamer Transcription Factor-3)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170803
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182398


  5 / 2890 MEDLINE  
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[PMID]:28727788
[Au] Autor:Malcher SM; Pieczarka JC; Geise L; Rossi RV; Pereira AL; O'Brien PCM; Asfora PH; Fonsêca da Silva V; Sampaio MI; Ferguson-Smith MA; Nagamachi CY
[Ad] Endereço:Centro de Estudos Avançados da Biodiversidade, Laboratório de Citogenética, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará, Brasil.
[Ti] Título:Oecomys catherinae (Sigmodontinae, Cricetidae): Evidence for chromosomal speciation?
[So] Source:PLoS One;12(7):e0181434, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Among the Oryzomyini (Sigmodontinae), Oecomys is the most speciose, with 17 species. This genus presents high karyotypic diversity (2n = 54 to 2n = 86) and many taxonomic issues at the species level because of the presence of cryptic species and the overlap of morphological characters. For these reasons the real number of species of Oecomys may be underestimated. With the aim of verifying if the taxon Oecomys catherinae is composed of more than one species, we made comparative studies on two populations from two regions of Brazil, one from the Amazon and another from the Atlantic Forest using both classical cytogenetics (G- and C-banding) and comparative genomic mapping with whole chromosome probes of Hylaeamys megacephalus (HME), molecular data (cytochrome b mitochondrial DNA) and morphology. Our results confirm that Oecomys catherinae occurs in the southeast Amazon, and reveal a new karyotype for the species (2n = 62, FNa = 62). The comparative genomic analysis with HME probes identified chromosomal homeologies between both populations and rearrangements that are responsible for the different karyotypes. We compared our results in Sigmodontinae genera with other studies that also used HME probes. These chromosomal differences together with the absence of consistent differentiation between the two populations on morphological and molecular analyses suggest that these populations may represent cryptic species.
[Mh] Termos MeSH primário: Arvicolinae/genética
Sigmodontinae/genética
[Mh] Termos MeSH secundário: Animais
Arvicolinae/anatomia & histologia
Brasil
Coloração Cromossômica
Cromossomos de Mamíferos
Feminino
Hibridização in Situ Fluorescente
Cariótipo
Cariotipagem
Masculino
Filogenia
Sigmodontinae/anatomia & histologia
Especificidade da Espécie
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170721
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0181434


  6 / 2890 MEDLINE  
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[PMID]:28719894
[Au] Autor:Poplavskaya NS; Romanenko SA; Serdyukova NA; Trifonov VA; Yang F; Nie W; Wang J; Bannikova AA; Surov AV; Lebedev VS
[Ad] Endereço:A.N. Severtsov Institute of Ecology and Evolution, RAS, Moscow State University, Moscow, Russia.
[Ti] Título:Karyotype Evolution and Phylogenetic Relationships of Cricetulus sokolovi Orlov et Malygin 1988 (Cricetidae, Rodentia) Inferred from Chromosomal Painting and Molecular Data.
[So] Source:Cytogenet Genome Res;152(2):65-72, 2017.
[Is] ISSN:1424-859X
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Sokolov's dwarf hamster (Cricetulus sokolovi) is the least studied representative of the striped hamsters (Cricetulus barabensis species group), the taxonomy of which remains controversial. The species was described based on chromosome morphology, but neither the details of the karyotype nor the phylogenetic relationships with other Cricetulus are known. In the present study, the karyotype of C. sokolovi was examined using cross-species chromosome painting. Molecular and cytogenetic data were employed to determine the phylogenetic position of Sokolov's hamster and to analyze the potential pathways of chromosome evolution in Cricetulus. Both the chromosome and molecular data support the species status of Sokolov's hamster. Phylogenetic analysis of the CYTB data placed C. sokolovi as sister to all other striped hamsters (sequence divergence of 8.1%). FISH data revealed that the karyotype of C. sokolovi is highly rearranged, with the most parsimonious scenario of its origin implying at least 4 robertsonian events and a centromere shift. Comparative cytogenetic data on Cricetinae suggest that their evolutionary history includes both periods of chromosomal conservatism and episodes of rapid chromosomal change.
[Mh] Termos MeSH primário: Coloração Cromossômica/métodos
Cromossomos de Mamíferos/genética
Cricetulus/genética
Cariótipo
Filogenia
[Mh] Termos MeSH secundário: Animais
Haplótipos/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170719
[St] Status:MEDLINE
[do] DOI:10.1159/000477521


  7 / 2890 MEDLINE  
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[PMID]:28708824
[Au] Autor:Crichton JH; Playfoot CJ; MacLennan M; Read D; Cooke HJ; Adams IR
[Ad] Endereço:MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine, University of Edinburgh, Western General Hospital, Edinburgh, United Kingdom.
[Ti] Título:Tex19.1 promotes Spo11-dependent meiotic recombination in mouse spermatocytes.
[So] Source:PLoS Genet;13(7):e1006904, 2017 Jul.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Meiosis relies on the SPO11 endonuclease to generate the recombinogenic DNA double strand breaks (DSBs) required for homologous chromosome synapsis and segregation. The number of meiotic DSBs needs to be sufficient to allow chromosomes to search for and find their homologs, but not excessive to the point of causing genome instability. Here we report that the mammal-specific gene Tex19.1 promotes Spo11-dependent recombination in mouse spermatocytes. We show that the chromosome asynapsis previously reported in Tex19.1-/- spermatocytes is preceded by reduced numbers of recombination foci in leptotene and zygotene. Tex19.1 is required for normal levels of early Spo11-dependent recombination foci during leptotene, but not for upstream events such as MEI4 foci formation or accumulation of H3K4me3 at recombination hotspots. Furthermore, we show that mice carrying mutations in Ubr2, which encodes an E3 ubiquitin ligase that interacts with TEX19.1, phenocopy the Tex19.1-/- recombination defects. These data suggest that Tex19.1 and Ubr2 are required for mouse spermatocytes to accumulate sufficient Spo11-dependent recombination to ensure that the homology search is consistently successful, and reveal a hitherto unknown genetic pathway promoting meiotic recombination in mammals.
[Mh] Termos MeSH primário: Endodesoxirribonucleases/metabolismo
Meiose/genética
Proteínas Nucleares/metabolismo
Espermatócitos/metabolismo
Ubiquitina-Proteína Ligases/metabolismo
[Mh] Termos MeSH secundário: Animais
Pareamento Cromossômico
Cromossomos de Mamíferos/genética
Cromossomos de Mamíferos/metabolismo
Endodesoxirribonucleases/genética
Masculino
Prófase Meiótica I/genética
Camundongos
Camundongos Endogâmicos C57BL
Proteínas Nucleares/genética
Regiões Promotoras Genéticas
Recombinação Genética
Ubiquitina-Proteína Ligases/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nuclear Proteins); 0 (Tex19 protein, mouse); EC 2.3.2.27 (UBR2 protein, mouse); EC 2.3.2.27 (Ubiquitin-Protein Ligases); EC 3.1.- (Endodeoxyribonucleases); EC 3.1.- (meiotic recombination protein SPO11)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170715
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1006904


  8 / 2890 MEDLINE  
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[PMID]:28682332
[Au] Autor:Nagano T; Lubling Y; Várnai C; Dudley C; Leung W; Baran Y; Mendelson Cohen N; Wingett S; Fraser P; Tanay A
[Ad] Endereço:Nuclear Dynamics Programme, The Babraham Insitute, Cambridge CB22 3AT, UK.
[Ti] Título:Cell-cycle dynamics of chromosomal organization at single-cell resolution.
[So] Source:Nature;547(7661):61-67, 2017 07 05.
[Is] ISSN:1476-4687
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Chromosomes in proliferating metazoan cells undergo marked structural metamorphoses every cell cycle, alternating between highly condensed mitotic structures that facilitate chromosome segregation, and decondensed interphase structures that accommodate transcription, gene silencing and DNA replication. Here we use single-cell Hi-C (high-resolution chromosome conformation capture) analysis to study chromosome conformations in thousands of individual cells, and discover a continuum of cis-interaction profiles that finely position individual cells along the cell cycle. We show that chromosomal compartments, topological-associated domains (TADs), contact insulation and long-range loops, all defined by bulk Hi-C maps, are governed by distinct cell-cycle dynamics. In particular, DNA replication correlates with a build-up of compartments and a reduction in TAD insulation, while loops are generally stable from G1 to S and G2 phase. Whole-genome three-dimensional structural models reveal a radial architecture of chromosomal compartments with distinct epigenomic signatures. Our single-cell data therefore allow re-interpretation of chromosome conformation maps through the prism of the cell cycle.
[Mh] Termos MeSH primário: Ciclo Celular/fisiologia
Cromossomos de Mamíferos/química
Cromossomos de Mamíferos/metabolismo
Epigênese Genética
Análise de Célula Única/métodos
[Mh] Termos MeSH secundário: Animais
Compartimento Celular
Ciclo Celular/genética
Cromossomos de Mamíferos/genética
Haploidia
Imagem Tridimensional
Camundongos
Células-Tronco Embrionárias Murinas/citologia
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171025
[Lr] Data última revisão:
171025
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170707
[St] Status:MEDLINE
[do] DOI:10.1038/nature23001


  9 / 2890 MEDLINE  
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[PMID]:28630119
[Au] Autor:Katayama S; Suzuki M; Yamaoka A; Keleku-Lukwete N; Katsuoka F; Otsuki A; Kure S; Engel JD; Yamamoto M
[Ad] Endereço:Department of Medical Biochemistry.
[Ti] Título:GATA2 haploinsufficiency accelerates EVI1-driven leukemogenesis.
[So] Source:Blood;130(7):908-919, 2017 Aug 17.
[Is] ISSN:1528-0020
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chromosomal rearrangements between 3q21 and 3q26 induce inappropriate expression by recruiting a -distal hematopoietic enhancer (G2DHE) to the proximity of the gene, leading to myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). The acquisition of G2DHE by the gene reciprocally deprives this enhancer of 1 of the 2 alleles, resulting in a loss-of-function genetic reduction in abundance. Because haploinsufficiency is strongly associated with MDS and AML, we asked whether misexpression and haploinsufficiency both contributed to the observed leukemogenesis by using a 3q21q26 mouse model that recapitulates the G2DHE-driven misexpression, but in this case, it was coupled to a heterozygous germ line deletion. Of note, the heterozygous deletion promoted the -provoked leukemic transformation, resulting in early onset of leukemia. The 3q21q26 mice suffered from leukemia in which B220 cells and/or Gr1 leukemic cells occupied their bone marrows. We found that the B220 Gr1 c-Kit population contained leukemia-initiating cells and supplied Gr1 leukemia cells in the 3q21q26 leukemia. When expression levels in the B220 Gr1 c-Kit cells were decreased as a result of heterozygous deletion or spontaneous phenomenon, myeloid differentiation of the B220 Gr1 c-Kit cells was suppressed, and the cells acquired induced proliferation as well as B-lymphoid-primed characteristics. Competitive transplantation analysis revealed that heterozygous deletion confers selective advantage to EVI1-expressing leukemia cell expansion in recipient mice. These results demonstrate that both the inappropriate stimulation of and the loss of 1 allele equivalent of expression contribute to the acceleration of leukemogenesis.
[Mh] Termos MeSH primário: Carcinogênese/patologia
Proteínas de Ligação a DNA/metabolismo
Fator de Transcrição GATA2/genética
Haploinsuficiência/genética
Leucemia/patologia
Fatores de Transcrição/metabolismo
[Mh] Termos MeSH secundário: Alelos
Animais
Carcinogênese/genética
Diferenciação Celular
Proliferação Celular
Cromossomos de Mamíferos/genética
Metabolismo Energético/genética
Regulação Leucêmica da Expressão Gênica
Células-Tronco Hematopoéticas/metabolismo
Leucemia/genética
Proteína do Locus do Complexo MDS1 e EVI1
Camundongos Endogâmicos C57BL
Modelos Biológicos
Transplante de Neoplasias
Células-Tronco Neoplásicas/metabolismo
Células-Tronco Neoplásicas/patologia
Fenótipo
Proteínas Proto-Oncogênicas c-kit/metabolismo
Proto-Oncogenes
Estresse Fisiológico/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA-Binding Proteins); 0 (GATA2 Transcription Factor); 0 (MDS1 and EVI1 Complex Locus Protein); 0 (Mecom protein, mouse); 0 (Transcription Factors); EC 2.7.10.1 (Proto-Oncogene Proteins c-kit)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170621
[St] Status:MEDLINE
[do] DOI:10.1182/blood-2016-12-756767


  10 / 2890 MEDLINE  
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[PMID]:28609438
[Au] Autor:Ye A; Kim H; Kim J
[Ad] Endereço:Department of Biological Sciences, Louisiana State University, Baton Rouge, LA, United States of America.
[Ti] Título:PEG3 control on the mammalian MSL complex.
[So] Source:PLoS One;12(6):e0178363, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Peg3 (paternally expressed gene 3) encodes a DNA-binding protein that functions as a transcriptional repressor. Recent studies revealed that PEG3 binds to Msl1 (male-specific lethal 1) and Msl3, the two main components of the MSL complex. In the current study, we investigated potential roles of Peg3 in controlling its downstream genes through H4K16ac, the histone modification by the MSL complex. According to the results, complete removal of PEG3 resulted in up-regulation of Msl1 and Msl3, and subsequently an increase in the global levels of H4K16ac, confirming PEG3 as a transcriptional repressor for MSL during mammalian development. Genome-wide analyses further revealed that about 10% of the entire gene catalogue was affected in the MEF cells lacking PEG3, displaying the increased levels of H4K16ac in their promoter regions. The expression levels of a small subset of the affected genes were up-regulated in the MEF cells lacking PEG3. Interestingly, three Hox clusters also exhibited changes in the levels of H4K16ac, suggesting potential roles of PEG3 and MSL in the regulation of Hox clusters. Overall, the current study reports that Peg3 may control its downstream genes through mammalian MSL.
[Mh] Termos MeSH primário: Proteínas Cromossômicas não Histona/metabolismo
Fibroblastos/metabolismo
Histona Acetiltransferases/metabolismo
Fatores de Transcrição Kruppel-Like/metabolismo
Mamíferos/metabolismo
[Mh] Termos MeSH secundário: Acetilação
Animais
Western Blotting
Células Cultivadas
Proteínas Cromossômicas não Histona/genética
Mapeamento Cromossômico
Cromossomos de Mamíferos/genética
Embrião de Mamíferos/citologia
Feminino
Fibroblastos/citologia
Expressão Gênica
Histona Acetiltransferases/genética
Histonas/genética
Histonas/metabolismo
Fatores de Transcrição Kruppel-Like/genética
Lisina/metabolismo
Masculino
Mamíferos/genética
Camundongos Endogâmicos C57BL
Camundongos Knockout
Reação em Cadeia da Polimerase Via Transcriptase Reversa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chromosomal Proteins, Non-Histone); 0 (Histones); 0 (Kruppel-Like Transcription Factors); 0 (Msl3 protein, mouse); 0 (Peg3 protein, mouse); EC 2.3.1.48 (Histone Acetyltransferases); EC 2.3.1.48 (MSL1 protein, mouse); K3Z4F929H6 (Lysine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170614
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0178363



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