Base de dados : MEDLINE
Pesquisa : A11.284.430.214.190.875.248.300 [Categoria DeCS]
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  1 / 695 MEDLINE  
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[PMID]:29053787
[Au] Autor:Devoy A; Kalmar B; Stewart M; Park H; Burke B; Noy SJ; Redhead Y; Humphrey J; Lo K; Jaeger J; Mejia Maza A; Sivakumar P; Bertolin C; Soraru G; Plagnol V; Greensmith L; Acevedo Arozena A; Isaacs AM; Davies B; Fratta P; Fisher EMC
[Ad] Endereço:Department of Neurodegenerative Disease, UCL Institute of Neurology, Queen Square, London WC1N 3BG, UK.
[Ti] Título:Humanized mutant FUS drives progressive motor neuron degeneration without aggregation in 'FUSDelta14' knockin mice.
[So] Source:Brain;140(11):2797-2805, 2017 Nov 01.
[Is] ISSN:1460-2156
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Mutations in FUS are causative for amyotrophic lateral sclerosis with a dominant mode of inheritance. In trying to model FUS-amyotrophic lateral sclerosis (ALS) in mouse it is clear that FUS is dosage-sensitive and effects arise from overexpression per se in transgenic strains. Novel models are required that maintain physiological levels of FUS expression and that recapitulate the human disease-with progressive loss of motor neurons in heterozygous animals. Here, we describe a new humanized FUS-ALS mouse with a frameshift mutation, which fulfils both criteria: the FUS Delta14 mouse. Heterozygous animals express mutant humanized FUS protein at physiological levels and have adult onset progressive motor neuron loss and denervation of neuromuscular junctions. Additionally, we generated a novel antibody to the unique human frameshift peptide epitope, allowing specific identification of mutant FUS only. Using our new FUSDelta14 ALS mouse-antibody system we show that neurodegeneration occurs in the absence of FUS protein aggregation. FUS mislocalization increases as disease progresses, and mutant FUS accumulates at the rough endoplasmic reticulum. Further, transcriptomic analyses show progressive changes in ribosomal protein levels and mitochondrial function as early disease stages are initiated. Thus, our new physiological mouse model has provided novel insight into the early pathogenesis of FUS-ALS.
[Mh] Termos MeSH primário: Esclerose Amiotrófica Lateral/genética
Modelos Animais de Doenças
Mutação da Fase de Leitura
Camundongos
Agregação Patológica de Proteínas/genética
Proteína FUS de Ligação a RNA/genética
[Mh] Termos MeSH secundário: Esclerose Amiotrófica Lateral/metabolismo
Animais
Retículo Endoplasmático Rugoso/metabolismo
Dosagem de Genes
Perfilação da Expressão Gênica
Técnicas de Introdução de Genes
Heterozigoto
Seres Humanos
Mitocôndrias/metabolismo
Neurônios Motores/metabolismo
Junção Neuromuscular/metabolismo
Agregação Patológica de Proteínas/metabolismo
Proteína FUS de Ligação a RNA/metabolismo
Proteínas Ribossômicas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA-Binding Protein FUS); 0 (Ribosomal Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171021
[St] Status:MEDLINE
[do] DOI:10.1093/brain/awx248


  2 / 695 MEDLINE  
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[PMID]:28658296
[Au] Autor:Castellanos N; Martínez LC; Silva EH; Teodoro AV; Serrão JE; Oliveira EE
[Ad] Endereço:Departamento de Entomologia, Universidade Federal de Viçosa, Viçosa-MG, Brasil.
[Ti] Título:Ultrastructural analysis of salivary glands in a phytophagous stink bug revealed the presence of unexpected muscles.
[So] Source:PLoS One;12(6):e0179478, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The exceptional abilities of stink bugs (Hemiptera: Pentatomidae) to colonize a diverse group of plants have been attributed to the feeding behaviors and the functions of the salivary complex of these insects. Here, we describe the ultrastructure of the salivary glands of the Neotropical brown stink bug, Euschistus heros, which is a major component of the pentatomid pest complex on soybeans, Glycine max, in the neotropics. Our results revealed a salivary gland complex consisting of two lobes (i.e., anterior and posterior), with a constriction between them (i.e., the hilum), in which the salivary and accessory gland ducts are inserted. The principal gland epithelium has a single layer of cells lining an enlarged lumen filled with saliva, and these cells are cuboidal, rich in rough endoplasmic reticulum and secretory vesicles, with well-developed nuclei, all of which are typical features of protein-secreting cells. We report, for the first time in insects, the presence of a layer of muscle cells surrounding the columnar hilum epithelium. The accessory salivary gland cells are cuboidal with nuclei containing condensed chromatin and cytoplasm rich in vacuoles and rough endoplasmic reticulum, indicating the potential involvement of these glands in water transport/secretion. The lumen content of each lobe of the principal gland suggests that the lobes produce different compounds. Thus, our results suggest that the E. heros salivary complex might have unconventional mechanisms to mix/release saliva, which might help explain the polyphagous abilities of these insects.
[Mh] Termos MeSH primário: Retículo Endoplasmático Rugoso/ultraestrutura
Heterópteros/ultraestrutura
Células Musculares/ultraestrutura
Glândulas Salivares/ultraestrutura
Vacúolos/ultraestrutura
[Mh] Termos MeSH secundário: Animais
Comportamento Alimentar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170629
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179478


  3 / 695 MEDLINE  
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[PMID]:28496033
[Au] Autor:Ding L; Zang L; Zhang Y; Zhang Y; Wang X; Ai W; Ding N; Wang H
[Ad] Endereço:School of Physical Education and Sport Sciences, Wenzhou Medical University, China.
[Ti] Título:Joint toxicity of fluoroquinolone and tetracycline antibiotics to zebrafish (Danio rerio) based on biochemical biomarkers and histopathological observation.
[So] Source:J Toxicol Sci;42(3):267-280, 2017.
[Is] ISSN:1880-3989
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Herein, we report on the joint toxicity of four fluoroquinolones and two tetracyclines (ß-diketone antibiotics-DKAs) to zebrafish based on a series of toxicological endpoints and histopathological observations. A positive dose-dependence was observed in DKA-exposure groups with a 72-hpf EC of 130.3 mg/L for hatching rate, 120-hpf LC of 149.8 mg/L, and 120-hpf EC of 135.1 mg/L for malformation rate. When zebrafish at 60 dpf were exposed to a series of DKA concentrations (45, 60 and 90 mg/L) for 7, 14 and 21 days, creatine kinase and AChE activities were significantly induced, and intracellular malondialdehyde increased in all treatments except for the 45 mg/L treatment. The transcription levels of AHRRa from livers were significantly (p < 0.05) up-regulated in all treatments after two months of DKA exposure. CKma expression from skeletal muscle was significantly down-regulated in the 90 mg/L treatment. A remarkable down-regulation of CYP3A65 was observed in the 60 mg/L treatment. DKA exposure resulted in severe tissue damage including mitochondria swelling, reduction of mitochondrial cristae, deepening of mitochondrial cristae bands, and decreasing and even disappearance of the rough endoplasmic reticulum. Total sperm motility was decreased by ca. 30% due to DKA exposure. These results provide important information for toxicity and health risks due to mixed DKA exposure in aquatic environments.
[Mh] Termos MeSH primário: Acetilcolinesterase/metabolismo
Antibacterianos/toxicidade
Hidrocarboneto de Aril Hidroxilases/genética
Hidrocarboneto de Aril Hidroxilases/metabolismo
Creatina Quinase/metabolismo
Fluoroquinolonas/toxicidade
Expressão Gênica/efeitos dos fármacos
Malondialdeído/metabolismo
Oxirredutases N-Desmetilantes/genética
Oxirredutases N-Desmetilantes/metabolismo
Motilidade Espermática/efeitos dos fármacos
Tetraciclinas/toxicidade
Proteínas de Peixe-Zebra/genética
Proteínas de Peixe-Zebra/metabolismo
[Mh] Termos MeSH secundário: Animais
Relação Dose-Resposta a Droga
Regulação para Baixo/efeitos dos fármacos
Retículo Endoplasmático Rugoso/efeitos dos fármacos
Fígado/metabolismo
Mitocôndrias/efeitos dos fármacos
Dilatação Mitocondrial/efeitos dos fármacos
Proteínas Repressoras/genética
Reprodução/efeitos dos fármacos
Transcrição Genética/efeitos dos fármacos
Peixe-Zebra
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Fluoroquinolones); 0 (Repressor Proteins); 0 (Tetracyclines); 0 (Zebrafish Proteins); 0 (aryl hydrocarbon receptor repressor 1, Danio rerio); 4Y8F71G49Q (Malondialdehyde); EC 1.14.14.1 (Aryl Hydrocarbon Hydroxylases); EC 1.14.14.1 (cytochrome P-450 CYP3A65, zebrafish); EC 1.5.- (Oxidoreductases, N-Demethylating); EC 2.7.3.2 (Creatine Kinase); EC 3.1.1.7 (Acetylcholinesterase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170513
[St] Status:MEDLINE
[do] DOI:10.2131/jts.42.267


  4 / 695 MEDLINE  
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[PMID]:28040618
[Au] Autor:Zhou Y; Jing W; Dahms HU; Hwang JS; Wang L
[Ad] Endereço:College of Life Science, Shanxi University, Taiyuan, Shanxi 030006, China.
[Ti] Título:Oxidative damage, ultrastructural alterations and gene expressions of hemocytes in the freshwater crab Sinopotamon henanense exposed to cadmium.
[So] Source:Ecotoxicol Environ Saf;138:130-138, 2017 Apr.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Toxicity of Cd was tested with the hemocytes of the freshwater crab, Sinopotamon henanense, which were exposed to concentrations of 0, 0.725, 1.450, and 2.900mgL Cd for 7, 14 and 21 d. We investigated the effects of Cd on the total antioxidant capacity (TAC), and oxidative damage of biomarkers, such as malondialdehyde (MDA), protein carbonyl derivates (PCO), and DNA-protein crosslink (DPC). Transmission electron microscopy (TEM) was applied to assess ultrastructural changes of hemocytes. The mRNA expression levels of prophenoloxidase (proPO), lysozyme (LSZ), metallothionein (MT), and the activity of phenoloxidase (PO) were also determined. Our results showed that TAC was inhibited by Cd, resulting in an increase of MDA contents, PCO contents, and DPC levels in hemocytes, respectively. Ultrastructural observations revealed that chromatin condensation, nucleus deformation, mitochondrial dilation, rough endoplasmatic reticulum (rER) degranulation and secondary or tertiary lysosomes were observed in hemocytes of crabs exposed to Cd. Meanwhile, the expression levels of proPO were down-regulated, while the activity of PO was up-regulated in hemocytes. The expression levels of LSZ and MT were up-regulated to some extent. Our findings suggest these parameters could be used as biomarkers in the monitoring of heavy metal pollution and quantitative risk assessments of pollutant exposure.
[Mh] Termos MeSH primário: Braquiúros
Cádmio/toxicidade
Hemócitos/efeitos dos fármacos
Hemócitos/ultraestrutura
RNA Mensageiro/metabolismo
[Mh] Termos MeSH secundário: Animais
Antioxidantes/metabolismo
Catecol Oxidase/genética
Núcleo Celular/ultraestrutura
Retículo Endoplasmático Rugoso/ultraestrutura
Precursores Enzimáticos/genética
Expressão Gênica/efeitos dos fármacos
Hemócitos/metabolismo
Lisossomos/ultraestrutura
Malondialdeído/metabolismo
Metalotioneína/genética
Mitocôndrias/ultraestrutura
Monofenol Mono-Oxigenase/metabolismo
Muramidase/genética
Carbonilação Proteica/efeitos dos fármacos
Poluentes Químicos da Água/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Enzyme Precursors); 0 (RNA, Messenger); 0 (Water Pollutants, Chemical); 00BH33GNGH (Cadmium); 4Y8F71G49Q (Malondialdehyde); 9038-94-2 (Metallothionein); EC 1.10.3.- (pro-phenoloxidase); EC 1.10.3.1 (Catechol Oxidase); EC 1.14.18.1 (Monophenol Monooxygenase); EC 3.2.1.17 (Muramidase)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170102
[St] Status:MEDLINE


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[PMID]:27785745
[Au] Autor:Koga D; Ushiki T; Watanabe T
[Ad] Endereço:Department of Microscopic Anatomy and Cell Biology, Asahikawa Medical University, Midorigaoka Higashi 2-1-1-1, Asahikawa, 078-8510, Japan. daisukek@asahikawa-med.ac.jp.
[Ti] Título:Novel scanning electron microscopy methods for analyzing the 3D structure of the Golgi apparatus.
[So] Source:Anat Sci Int;92(1):37-49, 2017 Jan.
[Is] ISSN:1447-073X
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:The structure of the Golgi apparatus has been extensively examined by light and electron microscopy, but details of its three-dimensional (3D) structure have remained unclear because of the technical limitations of conventional microscopy techniques. To overcome this problem, we have developed several novel scanning electron microscopy (SEM) methods for observing the 3D structure of subcellular organelles including the Golgi apparatus: (1) an osmium maceration method that facilitates SEM observation of membranous organelles, including the Golgi apparatus, by selectively removing soluble cytoplasmic proteins, (2) an osmium impregnation/maceration method that combines an osmium impregnation method with the osmium maceration method to determine the polarity of the Golgi apparatus by SEM, (3) a correlative light and SEM method that combines a cryosectioning technique with the osmium maceration method to enable correlation of the immunocytochemical distribution of molecules with the 3D ultrastructure of the Golgi apparatus, and (4) array tomography based on the systematic collection and integration of SEM images of serial ultrathin sections on glass slides for revealing the 3D ultrastructure of the entire Golgi apparatus. Together, the novel SEM techniques listed above can reveal the complete 3D structure of the Golgi apparatus in different cell types.
[Mh] Termos MeSH primário: Complexo de Golgi/ultraestrutura
Imagem Tridimensional/métodos
Microscopia Eletrônica de Varredura/métodos
[Mh] Termos MeSH secundário: Animais
Retículo Endoplasmático Rugoso/ultraestrutura
Gonadotrofos/citologia
Gonadotrofos/ultraestrutura
Mitocôndrias/ultraestrutura
Osmio
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
2E7M255OPY (Osmium)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170302
[Lr] Data última revisão:
170302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161028
[St] Status:MEDLINE


  6 / 695 MEDLINE  
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[PMID]:27744458
[Au] Autor:Al-Qudah MA; Al-Dwairi A
[Ad] Endereço:Department of Physiology, Jordan University of Science and Technology, Irbid, Jordan. E-mail: maalqudah7@just.edu.jo.
[Ti] Título:Mechanisms and regulation of neurotrophin synthesis and secretion.
[So] Source:Neurosciences (Riyadh);21(4):306-313, 2016 Oct.
[Is] ISSN:1319-6138
[Cp] País de publicação:Saudi Arabia
[La] Idioma:eng
[Ab] Resumo:Neurotrophins are secreted proteins that are synthesized as pre-pro-neurotrophins on the rough endoplasmic reticulum, which are subsequently processed and then secreted as mature proteins. During synthesis, neurotrophins are sorted in the trans-Golgi apparatus into 2 pathways of secretion; the constitutive and the regulated pathways. Neurotrophins in the constitutive pathway are secreted cautiously without any trigger, while in the regulated pathway of secretion an external stimulus elevates the calcium concentration intracellularly leading to neurotrophin release. The regulation of sorting and secretion of neurotrophins is critical for several processes in the body, such as synaptic plasticity, neurodegenerative disorders, demyelination disease, and inflammation. The purpose of this review is to summarize the current mechanisms of neurotrophin sorting and secretion.
[Mh] Termos MeSH primário: Doenças Desmielinizantes/metabolismo
Fatores de Crescimento Neural/secreção
Doenças Neurodegenerativas/metabolismo
Plasticidade Neuronal
[Mh] Termos MeSH secundário: Sinalização do Cálcio
Retículo Endoplasmático Rugoso/metabolismo
Seres Humanos
Inflamação
Fatores de Crescimento Neural/biossíntese
Rede trans-Golgi/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Nerve Growth Factors)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170620
[Lr] Data última revisão:
170620
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161017
[St] Status:MEDLINE
[do] DOI:10.17712/nsj.2016.4.20160080


  7 / 695 MEDLINE  
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[PMID]:27437400
[Au] Autor:Nakadate K; Motojima K; Hirakawa T; Tanaka-Nakadate S
[Ad] Endereço:Department of Basic Science, Educational and Research Center for Pharmacy, Meiji Pharmaceutical University, Tokyo 204-8588, Japan.
[Ti] Título:Progressive Depletion of Rough Endoplasmic Reticulum in Epithelial Cells of the Small Intestine in Monosodium Glutamate Mice Model of Obesity.
[So] Source:Biomed Res Int;2016:5251738, 2016.
[Is] ISSN:2314-6141
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chronic obesity is a known risk factor for metabolic syndrome. However, little is known about pathological changes in the small intestine associated with chronic obesity. This study investigated cellular and subcellular level changes in the small intestine of obese mice. In this study, a mouse model of obesity was established by early postnatal administration of monosodium glutamate. Changes in body weight were monitored, and pathological changes in the small intestine were evaluated using hematoxylin-eosin and Nissl staining and light and electron microscopy. Consequently, obese mice were significantly heavier compared with controls from 9 weeks of age. Villi in the small intestine of obese mice were elongated and thinned. There was reduced hematoxylin staining in the epithelium of the small intestine of obese mice. Electron microscopy revealed a significant decrease in and shortening of rough endoplasmic reticulum in epithelial cells of the small intestine of obese mice compared with normal mice. The decrease in rough endoplasmic reticulum in the small intestine epithelial cells of obese mice indicates that obesity starting in childhood influences various functions of the small intestine, such as protein synthesis, and could impair both the defense mechanism against invasion of pathogenic microbes and nutritional absorption.
[Mh] Termos MeSH primário: Peso Corporal/fisiologia
Retículo Endoplasmático Rugoso/ultraestrutura
Intestino Delgado/ultraestrutura
Obesidade/fisiopatologia
[Mh] Termos MeSH secundário: Animais
Peso Corporal/efeitos dos fármacos
Modelos Animais de Doenças
Retículo Endoplasmático Rugoso/metabolismo
Células Epiteliais/efeitos dos fármacos
Células Epiteliais/metabolismo
Células Epiteliais/ultraestrutura
Seres Humanos
Intestino Delgado/metabolismo
Camundongos
Camundongos Obesos
Obesidade/induzido quimicamente
Obesidade/metabolismo
Glutamato de Sódio/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
W81N5U6R6U (Sodium Glutamate)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160721
[St] Status:MEDLINE
[do] DOI:10.1155/2016/5251738


  8 / 695 MEDLINE  
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[PMID]:27120118
[Au] Autor:Jin LQ; Pennise CR; Rodemer W; Jahn KS; Selzer ME
[Ad] Endereço:Shriners Hospitals Pediatric Research Center, Lewis Katz School of Medicine at Temple University, 3500 North Broad Street, Philadelphia, PA, 19140. jinliqin@temple.edu.
[Ti] Título:Protein synthetic machinery and mRNA in regenerating tips of spinal cord axons in lamprey.
[So] Source:J Comp Neurol;524(17):3614-3640, 2016 Dec 01.
[Is] ISSN:1096-9861
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Polyribosomes, mRNA, and other elements of translational machinery have been reported in peripheral nerves and in elongating injured axons of sensory neurons in vitro, primarily in growth cones. Evidence for involvement of local protein synthesis in regenerating central nervous system (CNS) axons is less extensive. We monitored regeneration of back-labeled lamprey spinal axons after spinal cord transection and detected mRNA in axon tips by in situ hybridization and microaspiration of their axoplasm. Poly(A)+mRNA was present in the axon tips, and was more abundant in actively regenerating tips than in static or retracting ones. Target-specific polymerase chain reaction (PCR) and in situ hybridization revealed plentiful mRNA for the low molecular neurofilament subunit and ß-tubulin, but very little for ß-actin, consistent with the morphology of their tips, which lack filopodia and lamellipodia. Electron microscopy showed ribosomes/polyribosomes in the distal parts of axon tips and in association with vesicle-like membranes, primarily in the tip. In one instance, there were structures with the appearance of rough endoplasmic reticulum. Immunohistochemistry showed patches of ribosomal protein S6 positivity in a similar distribution. The results suggest that local protein synthesis might be involved in the mechanism of axon regeneration in the lamprey spinal cord. J. Comp. Neurol. 524:3614-3640, 2016. © 2016 Wiley Periodicals, Inc.
[Mh] Termos MeSH primário: Axônios/metabolismo
Lampreias/metabolismo
Regeneração Nervosa/fisiologia
Biossíntese de Proteínas/fisiologia
Medula Espinal/metabolismo
[Mh] Termos MeSH secundário: Actinas/metabolismo
Animais
Axônios/ultraestrutura
Western Blotting
Clonagem Molecular
Citoplasma/metabolismo
Citoesqueleto/metabolismo
Retículo Endoplasmático Rugoso/metabolismo
Retículo Endoplasmático Rugoso/ultraestrutura
Proteínas de Peixes/metabolismo
Proteínas de Peixes/ultraestrutura
Hibridização In Situ
Microscopia Eletrônica
Proteínas de Neurofilamentos/metabolismo
Reação em Cadeia da Polimerase
Polirribossomos/metabolismo
Polirribossomos/ultraestrutura
RNA Mensageiro/metabolismo
Proteína S6 Ribossômica/metabolismo
Proteína S6 Ribossômica/ultraestrutura
Medula Espinal/ultraestrutura
Tubulina (Proteína)/metabolismo
Vimentina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Actins); 0 (Fish Proteins); 0 (Neurofilament Proteins); 0 (RNA, Messenger); 0 (Ribosomal Protein S6); 0 (Tubulin); 0 (Vimentin); 0 (neurofilament protein L)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160428
[St] Status:MEDLINE
[do] DOI:10.1002/cne.24020


  9 / 695 MEDLINE  
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[PMID]:26821870
[Au] Autor:Yoo H; Kim E; Hwang SU; Yoon JD; Jeon Y; Park KM; Kim KJ; Jin M; Lee CK; Lee E; Kim H; Kim G; Hyun SH
[Ad] Endereço:Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea.
[Ti] Título:Ultrastructural comparison of porcine putative embryonic stem cells derived by in vitro fertilization and somatic cell nuclear transfer.
[So] Source:J Reprod Dev;62(2):177-85, 2016 Apr 22.
[Is] ISSN:1348-4400
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:The ultrastructure of porcine putative embryonic stem cells and porcine fetal fibroblasts (PFFs) was analyzed by transmission electron microscopy. The aim of this study was to compare the features of organelles in in vitro fertilization (IVF) derived porcine embryonic stem cells (IVF-pESCs) and somatic cell nuclear transfer (SCNT) derived pESCs (SCNT-pESCs). Also, the features of organelles in high-passage IVF-pESCs were compared with those in low-passage cells. The ultrastructure of PFFs showed rare microvilli on the cell surfaces, polygonal or irregular nuclei with one to two reticular-shaped nucleoli and euchromatin, low cytoplasm-to-nucleus ratios, rare ribosomes, rare rough endoplasmic reticulum, elongated mitochondria, rich lysosomes and rich phagocytic vacuoles. IVF-pESCs showed rare microvilli on the cell surfaces, round or irregular nuclei with one to two reticular-shaped nucleoli and euchromatin, low cytoplasm-to-nucleus ratios, rich ribosomes, long stacks of rough endoplasmic reticulum, elongated mitochondria, rare lysosomes and rare autophagic vacuoles. By contrast, SCNT-pESCs showed rich microvilli with various lengths and frequencies on the cell surfaces, polygonal nuclei with one reticular shaped nucleoli and heterochromatin, high cytoplasm-to-nucleus ratios, rare ribosomes, rare rough endoplasmic reticulum, round mitochondria, rich lysosomes and rich phagocytic vacuoles with clear intercellular junctions. Furthermore, high-passage IVF-pESCs showed irregularly shaped colonies, pyknosis and numerous lysosomes associated with autophagic vacuoles showing signs of apoptosis. In conclusion, this study confirms that the ultrastructural characteristics of pESCs differ depending on their origin. These ultrastructural characteristics might be useful in biomedical research using pESCs, leading to new insights regarding regenerative medicine and tissue repair.
[Mh] Termos MeSH primário: Células-Tronco Embrionárias/ultraestrutura
Fertilização In Vitro/métodos
Técnicas de Transferência Nuclear
[Mh] Termos MeSH secundário: Animais
Apoptose
Blastocisto/citologia
Linhagem Celular
Núcleo Celular/ultraestrutura
Técnicas de Cocultura
Citoplasma/ultraestrutura
Células-Tronco Embrionárias/citologia
Retículo Endoplasmático Rugoso/ultraestrutura
Fibroblastos/ultraestrutura
Camundongos
Camundongos Endogâmicos ICR
Microscopia Eletrônica de Transmissão
Microvilosidades/ultraestrutura
Mitocôndrias/ultraestrutura
Fagocitose
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170616
[Lr] Data última revisão:
170616
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160130
[St] Status:MEDLINE
[do] DOI:10.1262/jrd.2015-124


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[PMID]:26454638
[Au] Autor:Plachno BJ; Kurczynska E; Swiatek P
[Ad] Endereço:Department of Plant Cytology and Embryology, Jagiellonian University in Kraków, 9 Gronostajowa St., 30-387, Kraków, Poland. bartosz.plachno@uj.edu.pl.
[Ti] Título:Integument cell differentiation in dandelions (Taraxacum, Asteraceae, Lactuceae) with special attention paid to plasmodesmata.
[So] Source:Protoplasma;253(5):1365-72, 2016 Sep.
[Is] ISSN:1615-6102
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:The aim of the paper is to determine what happens with plasmodesmata when mucilage is secreted into the periplasmic space in plant cells. Ultrastructural analysis of the periendothelial zone mucilage cells was performed on examples of the ovule tissues of several sexual and apomictic Taraxacum species. The cytoplasm of the periendothelial zone cells was dense, filled by numerous organelles and profiles of rough endoplasmic reticulum and active Golgi dictyosomes with vesicles that contained fibrillar material. At the beginning of the differentiation process of the periendothelial zone, the cells were connected by primary plasmodesmata. However, during the differentiation and the thickening of the cell walls (mucilage deposition), the plasmodesmata become elongated and associated with cytoplasmic bridges. The cytoplasmic bridges may connect the protoplast to the plasmodesmata through the mucilage layers in order to maintain cell-to-cell communication during the differentiation of the periendothelial zone cells.
[Mh] Termos MeSH primário: Diferenciação Celular
Citoplasma/metabolismo
Mucilagem Vegetal/metabolismo
Plasmodesmos/ultraestrutura
Taraxacum/citologia
Taraxacum/ultraestrutura
[Mh] Termos MeSH secundário: Comunicação Celular
Parede Celular/metabolismo
Retículo Endoplasmático Rugoso/fisiologia
Complexo de Golgi/fisiologia
Periplasma/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Mucilage)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170404
[Lr] Data última revisão:
170404
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151012
[St] Status:MEDLINE
[do] DOI:10.1007/s00709-015-0894-2



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