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Pesquisa : A11.284.430.214.190.875.700.140 [Categoria DeCS]
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  1 / 15349 MEDLINE  
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[PMID]:29342197
[Au] Autor:Talukder SK; Azhaguvel P; Chekhovskiy K; Saha MC
[Ad] Endereço:Noble Research Institute, LLC, Ardmore, OK, United States of America.
[Ti] Título:Molecular discrimination of tall fescue morphotypes in association with Festuca relatives.
[So] Source:PLoS One;13(1):e0191343, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tall fescue (Festuca arundinacea Schreb.) is an important cool-season perennial grass species used as forage and turf, and in conservation plantings. There are three morphotypes in hexaploid tall fescue: Continental, Mediterranean and Rhizomatous. This study was conducted to develop morphotype-specific molecular markers to distinguish Continental and Mediterranean tall fescues, and establish their relationships with other species of the Festuca genus for genomic inference. Chloroplast sequence variation and simple sequence repeat (SSR) polymorphism were explored in 12 genotypes of three tall fescue morphotypes and four Festuca species. Hypervariable chloroplast regions were retrieved by using 33 specifically designed primers followed by sequencing the PCR products. SSR polymorphism was studied using 144 tall fescue SSR primers. Four chloroplast (NFTCHL17, NFTCHL43, NFTCHL45 and NFTCHL48) and three SSR (nffa090, nffa204 and nffa338) markers were identified which can distinctly differentiate Continental and Mediterranean morphotypes. A primer pair, NFTCHL45, amplified a 47 bp deletion between the two morphotypes is being routinely used in the Noble Research Institute's core facility for morphotype discrimination. Both chloroplast sequence variation and SSR diversity showed a close association between Rhizomatous and Continental morphotypes, while the Mediterranean morphotype was in a distant clade. F. pratensis and F. arundinacea var. glaucescens, the P and G1G2 genome donors, respectively, were grouped with the Continental clade, and F. mairei (M1M2 genome) grouped with the Mediterranean clade in chloroplast sequence variation, while both F. pratensis and F. mairei formed independent clade in SSR analysis. Age estimation based on chloroplast sequence variation indicated that the Continental and Mediterranean clades might have been colonized independently during 0.65 ± 0.06 and 0.96 ± 0.1 million years ago (Mya) respectively. The findings of the study will enhance tall fescue breeding for persistence and productivity.
[Mh] Termos MeSH primário: Cloroplastos/genética
Festuca/genética
[Mh] Termos MeSH secundário: DNA de Plantas/genética
Festuca/metabolismo
Variação Genética/genética
Genômica/métodos
Genótipo
Repetições de Microssatélites/genética
Filogenia
Poaceae/genética
Análise de Sequência de DNA/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Plant)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180118
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191343


  2 / 15349 MEDLINE  
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[PMID]:29111476
[Au] Autor:Lopes JC; Chatrou LW; Mello-Silva R; Rudall PJ; Sajo MG
[Ad] Endereço:Universidade de São Paulo, Instituto de Biociências, Departamento de Botânica, Rua do Matão, 277, 05508-090 São Paulo, SP, Brazil. Electronic address: jenifer.clopes@gmail.com.
[Ti] Título:Phylogenomics and evolution of floral traits in the Neotropical tribe Malmeeae (Annonaceae).
[So] Source:Mol Phylogenet Evol;118:379-391, 2018 Jan.
[Is] ISSN:1095-9513
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Androdioecy is the rarest sexual system among plants. The majority of androdioecious species are herbaceous plants that have evolved from dioecious ancestors. Nevertheless, some woody and androdioecious plants have hermaphrodite ancestors, as in the Annonaceae, where androdioecious genera have arisen several times in different lineages. The majority of androdioecious species of Annonaceae belong to the Neotropical tribe Malmeeae. In addition to these species, Pseudoxandra spiritus-sancti was recently confirmed to be androdioecious. Here, we describe the morphology of male and bisexual flowers of Pseudoxandra spiritus-sancti, and investigate the evolution of androdioecy in Malmeeae. The phylogeny of tribe Malmeeae was reconstructed using Bayesian inference, maximum parsimony and maximum likelihood of 32 taxa, using DNA sequences of 66 molecular markers of the chloroplast genome, sequenced by next generation sequencing. The reconstruction of ancestral states was performed for characters associated with sexual systems and floral morphology. The phylogenetic analyses reconstructed three main groups in Malmeeae, (Malmea (Cremastosperma, Pseudoxandra)) sister to the rest of the tribe, and (Unonopsis (Bocageopsis, Onychopetalum)) sister to (Mosannona, Ephedranthus, Klarobelia, Oxandra, Pseudephedranthus fragrans, Pseudomalmea, Ruizodendron ovale). Hermaphroditism is plesiomorphic in the tribe, with four independent evolutions of androdieocy, which represents a synapomorphy of two groups, one that includes three genera and 14 species, the other with a single genus of seven species. Male flowers are unisexual from inception and bisexual flowers possess staminodes and functional stamens. Pseudoxandra spiritus-sancti is structurally androdioecious.
[Mh] Termos MeSH primário: Annonaceae/classificação
[Mh] Termos MeSH secundário: Annonaceae/anatomia & histologia
Annonaceae/genética
Teorema de Bayes
Evolução Biológica
Cloroplastos/genética
DNA de Cloroplastos/química
DNA de Cloroplastos/isolamento & purificação
DNA de Cloroplastos/metabolismo
Flores/anatomia & histologia
Flores/genética
Funções Verossimilhança
Microscopia Eletrônica de Varredura
Fenótipo
Filogenia
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Chloroplast)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171108
[St] Status:MEDLINE


  3 / 15349 MEDLINE  
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[PMID]:28464535
[Au] Autor:Huttanus HM; Feng X
[Ad] Endereço:Biological Systems Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.
[Ti] Título:Compartmentalized metabolic engineering for biochemical and biofuel production.
[So] Source:Biotechnol J;12(6), 2017 Jun.
[Is] ISSN:1860-7314
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Sub-cellular compartments create specialized reaction chambers in eukaryotes. These compartments provide favorable micro-environments for many metabolic processes. Recently, metabolic engineers have explored the concept of pathway compartmentalization to enhance the performance of metabolic pathways. This strategy offers many unique advantages, including (i) increased local concentrations of enzymes and substrates, (ii) accessing alternate substrate pools, (iii) separation from competing reactions, and (iv) isolation of harmful intermediates or conditions needed for the pathway. In this review, the method of localizing metabolic pathways into specific organelles as well as the benefits of pathway compartmentalization in terms of enhancing the production of value-added chemicals is discussed.
[Mh] Termos MeSH primário: Biocombustíveis
Engenharia Metabólica
Redes e Vias Metabólicas
[Mh] Termos MeSH secundário: Bactérias/metabolismo
Compartimento Celular
Cloroplastos/metabolismo
Mitocôndrias/metabolismo
Organelas/metabolismo
Peroxissomos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Biofuels)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1002/biot.201700052


  4 / 15349 MEDLINE  
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[PMID]:29293597
[Au] Autor:Silva SR; Michael TP; Meer EJ; Pinheiro DG; Varani AM; Miranda VFO
[Ad] Endereço:Universidade Estadual Paulista (Unesp), Botucatu, Instituto de Biociências, São Paulo, Brazil.
[Ti] Título:Comparative genomic analysis of Genlisea (corkscrew plants-Lentibulariaceae) chloroplast genomes reveals an increasing loss of the ndh genes.
[So] Source:PLoS One;13(1):e0190321, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In the carnivorous plant family Lentibulariaceae, all three genome compartments (nuclear, chloroplast, and mitochondria) have some of the highest rates of nucleotide substitutions across angiosperms. While the genera Genlisea and Utricularia have the smallest known flowering plant nuclear genomes, the chloroplast genomes (cpDNA) are mostly structurally conserved except for deletion and/or pseudogenization of the NAD(P)H-dehydrogenase complex (ndh) genes known to be involved in stress conditions of low light or CO2 concentrations. In order to determine how the cpDNA are changing, and to better understand the evolutionary history within the Genlisea genus, we sequenced, assembled and analyzed complete cpDNA from six species (G. aurea, G. filiformis, G. pygmaea, G. repens, G. tuberosa and G. violacea) together with the publicly available G. margaretae cpDNA. In general, the cpDNA structure among the analyzed Genlisea species is highly similar. However, we found that the plastidial ndh genes underwent a progressive process of degradation similar to the other terrestrial Lentibulariaceae cpDNA analyzed to date, but in contrast to the aquatic species. Contrary to current thinking that the terrestrial environment is a more stressful environment and thus requiring the ndh genes, we provide evidence that in the Lentibulariaceae the terrestrial forms have progressive loss while the aquatic forms have the eleven plastidial ndh genes intact. Therefore, the Lentibulariaceae system provides an important opportunity to understand the evolutionary forces that govern the transition to an aquatic environment and may provide insight into how plants manage water stress at a genome scale.
[Mh] Termos MeSH primário: Cloroplastos/genética
Genoma de Cloroplastos
Magnoliopsida/genética
NADPH Desidrogenase/genética
[Mh] Termos MeSH secundário: Magnoliopsida/classificação
Filogenia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
EC 1.6.99.1 (NADPH Dehydrogenase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190321


  5 / 15349 MEDLINE  
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[PMID]:29229384
[Au] Autor:Yang J; Zhang M; Wang X
[Ad] Endereço:School of Life Sciences, Anhui University, 111 Jiulong Road, Hefei, Anhui 230601, China.
[Ti] Título:Crystal structure of the chloroplast RNA editing factor MORF2.
[So] Source:Biochem Biophys Res Commun;495(2):2038-2043, 2018 01 08.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:RNA editing is a post-transcription process that alters the genetic information on RNA molecules. In plastids and mitochondria of flowering plants, the multiple organellar RNA editing factors (MORFs) interact with the PLS-type pentatricopeptide repeat (PPR) proteins and participate in RNA editing of cytidine-to-uridine conversion. The PPR proteins recognize cytidine targets around the editing sites, and the MORF proteins modulate the RNA-binding activity of the PPR proteins. Here, we report the structure of the Arabidopsis thaliana chloroplast MORF2 at 2.4 Å resolution. The structure, adopting typical MORF-box fold as observed in mitochondrial MORF1 and chloroplast MORF9, reveals an MORF1-like dimerization mode. The difference between the two dimerization modes can be attributed to F157 (corresponding F162 in MORF1 and W160 in MORF9), which causes a 60° shift upon dimerization. This observation, together with the PPR-MORF2 model, suggests a dimer-to-monomer transition during RNA editosome formation.
[Mh] Termos MeSH primário: Arabidopsis/química
Arabidopsis/ultraestrutura
Cloroplastos/ultraestrutura
RNA de Cloroplastos/ultraestrutura
[Mh] Termos MeSH secundário: Proteínas de Arabidopsis
Cloroplastos/química
Proteínas Mitocondriais
Conformação Proteica
Edição de RNA
RNA de Cloroplastos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (MORF2 protein, Arabidopsis); 0 (Mitochondrial Proteins); 0 (RNA, Chloroplast)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE


  6 / 15349 MEDLINE  
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[PMID]:29281724
[Au] Autor:Jiang T; Oh ES; Bonea D; Zhao R
[Ad] Endereço:Departments of Biological Sciences and Cell & Systems Biology, University of Toronto, Toronto, Ontario, Canada.
[Ti] Título:HSP90C interacts with PsbO1 and facilitates its thylakoid distribution from chloroplast stroma in Arabidopsis.
[So] Source:PLoS One;12(12):e0190168, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Arabidopsis plastidic HSP90C is an HSP90 family molecular chaperone that is required for chloroplast development and function. To understand the mechanism of action of HSP90C within the chloroplast, we conducted a yeast two-hybrid screening and revealed it interacts directly with the photosystem II extrinsic protein PsbO1, which performs a canonical function in the thylakoid lumen. To understand the biological significance of HSP90C-PsbO1 interaction, we investigated the role of HSP90C in modulating the stromal and thylakoid distribution of PsbO1GFP fusion protein. Fusion to GFP significantly delays the PsbO1 thylakoid transport and induces a variegation phenotype. Overexpression of HSP90C promotes the thylakoid distribution of PsbO1GFP and alleviates the leaf variegation. By tracking the chloroplast maturation during photomorphogenesis, we observed PsbO1GFP tends to form distinct fluorescent clusters within the stroma with delayed thylakoid membrane biogenesis, while HSP90C overexpression corrects these adverse effects. We also demonstrated that active HSP90C function is specifically required for stable accumulation of mature PsbO1GFP in thylakoid by using specific inhibitor geldanamycin. This study therefore not only identified novel HSP90C interactors, but also reports for the first time that PsbO1 enroute from the cytoplasm to thylakoid lumen is tightly regulated by the HSP90C chaperone complex in plastid stroma; whereas the proper HSP90C homeostasis is also critical for chloroplast maturation and function.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/metabolismo
Arabidopsis/metabolismo
Cloroplastos/metabolismo
Proteínas de Choque Térmico/metabolismo
Tilacoides/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Heat-Shock Proteins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171228
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190168


  7 / 15349 MEDLINE  
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[PMID]:29191002
[Au] Autor:Nain-Perez A; Barbosa LCA; Maltha CRA; Giberti S; Forlani G
[Ad] Endereço:Department of Chemistry, Universidade Federal de Minas Gerais , Av. Pres. Antônio Carlos, 6627, Campus Pampulha, CEP 31270-901, Belo Horizonte, MG Brazil.
[Ti] Título:Tailoring Natural Abenquines To Inhibit the Photosynthetic Electron Transport through Interaction with the D1 Protein in Photosystem II.
[So] Source:J Agric Food Chem;65(51):11304-11311, 2017 Dec 27.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Abenquines are natural N-acetylaminobenzoquinones bearing amino acid residues, which act as weak inhibitors of the photosynthetic electron transport chain. Aiming to exploit the abenquine scaffold as a model for the synthesis of new herbicides targeting photosynthesis, 14 new analogues were prepared by replacing the amino acid residue with benzylamines and the acetyl with different acyl groups. The synthesis was accomplished in three steps with a 68-95% overall yield from readily available 2,5-dimethoxyaniline, acyl chlorides, and benzyl amines. Key steps include (i) acylation of the aniline, (ii) oxidation, and (iii) oxidative addition of the benzylamino moiety. The compounds were assayed for their activity as Hill inhibitors, under basal, uncoupled, or phosphorylating conditions, or excluding photosystem I. Four analogues showed high effectiveness (IC = 0.1-0.4 µM), comparable with the commercial herbicide diuron (IC = 0.3 µM). The data suggest that this class of compounds interfere at the reducing side of photosystem II, having protein D1 as the most probable target. Molecular docking studies with the plastoquinone binding site of Spinacia oleracea further strengthened this proposal.
[Mh] Termos MeSH primário: Benzoquinonas/farmacologia
Transporte de Elétrons/efeitos dos fármacos
Herbicidas/farmacologia
Fotossíntese/efeitos dos fármacos
Complexo de Proteína do Fotossistema II/metabolismo
Spinacia oleracea/metabolismo
[Mh] Termos MeSH secundário: Benzoquinonas/química
Cloroplastos/efeitos dos fármacos
Cloroplastos/metabolismo
Herbicidas/química
Simulação de Acoplamento Molecular
Spinacia oleracea/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzoquinones); 0 (Herbicides); 0 (Photosystem II Protein Complex)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171202
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04624


  8 / 15349 MEDLINE  
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[PMID]:29223152
[Au] Autor:Cherepanov DA; Milanovsky GE; Petrova AA; Tikhonov AN; Semenov AY
[Ad] Endereço:Lomonosov Moscow State University, Belozersky Institute of Physico-Chemical Biology, Moscow, 119992, Russia. tscherepanov@gmail.com.
[Ti] Título:Electron Transfer through the Acceptor Side of Photosystem I: Interaction with Exogenous Acceptors and Molecular Oxygen.
[So] Source:Biochemistry (Mosc);82(11):1249-1268, 2017 Nov.
[Is] ISSN:1608-3040
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This review considers the state-of-the-art on mechanisms and alternative pathways of electron transfer in photosynthetic electron transport chains of chloroplasts and cyanobacteria. The mechanisms of electron transport control between photosystems (PS) I and II and the Calvin-Benson cycle are considered. The redistribution of electron fluxes between the noncyclic, cyclic, and pseudocyclic pathways plays an important role in the regulation of photosynthesis. Mathematical modeling of light-induced electron transport processes is considered. Particular attention is given to the electron transfer reactions on the acceptor side of PS I and to interactions of PS I with exogenous acceptors, including molecular oxygen. A kinetic model of PS I and its interaction with exogenous electron acceptors has been developed. This model is based on experimental kinetics of charge recombination in isolated PS I. Kinetic and thermodynamic parameters of the electron transfer reactions in PS I are scrutinized. The free energies of electron transfer between quinone acceptors A /A in the symmetric redox cofactor branches of PS I and iron-sulfur clusters F , F , and F have been estimated. The second-order rate constants of electron transfer from PS I to external acceptors have been determined. The data suggest that byproduct formation of superoxide radical in PS I due to the reduction of molecular oxygen in the A site (Mehler reaction) can exceed 0.3% of the total electron flux in PS I.
[Mh] Termos MeSH primário: Transporte de Elétrons
Complexo de Proteína do Fotossistema I/metabolismo
[Mh] Termos MeSH secundário: Cloroplastos/química
Cloroplastos/metabolismo
Cianobactérias/química
Cianobactérias/metabolismo
Proteínas com Ferro-Enxofre/metabolismo
Cinética
Modelos Químicos
Oxigênio/metabolismo
Quinonas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Iron-Sulfur Proteins); 0 (Photosystem I Protein Complex); 0 (Quinones); S88TT14065 (Oxygen)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180103
[Lr] Data última revisão:
180103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171211
[St] Status:MEDLINE
[do] DOI:10.1134/S0006297917110037


  9 / 15349 MEDLINE  
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[PMID]:28470336
[Au] Autor:Otori K; Tanabe N; Maruyama T; Sato S; Yanagisawa S; Tamoi M; Shigeoka S
[Ad] Endereço:Department of Advanced Bioscience, Faculty of Agriculture, Kindai University, Nakamachi, Nara, 631-8505, Japan.
[Ti] Título:Enhanced photosynthetic capacity increases nitrogen metabolism through the coordinated regulation of carbon and nitrogen assimilation in Arabidopsis thaliana.
[So] Source:J Plant Res;130(5):909-927, 2017 Sep.
[Is] ISSN:1618-0860
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Plant growth and productivity depend on interactions between the metabolism of carbon and nitrogen. The sensing ability of internal carbon and nitrogen metabolites (the C/N balance) enables plants to regulate metabolism and development. In order to investigate the effects of an enhanced photosynthetic capacity on the metabolism of carbon and nitrogen in photosynthetically active tissus (source leaves), we herein generated transgenic Arabidopsis thaliana plants (ApFS) that expressed cyanobacterial fructose-1,6-/sedoheptulose-1,7-bisphosphatase in their chloroplasts. The phenotype of ApFS plants was indistinguishable from that of wild-type plants at the immature stage. However, as plants matured, the growth of ApFS plants was superior to that of wild-type plants. Starch levels were higher in ApFS plants than in wild-type plants at 2 and 5 weeks. Sucrose levels were also higher in ApFS plants than in wild-type plants, but only at 5 weeks. On the other hand, the contents of various free amino acids were lower in ApFS plants than in wild-type plants at 2 weeks, but were similar at 5 weeks. The total C/N ratio was the same in ApFS plants and wild-type plants, whereas nitrite levels increased in parallel with elevations in nitrate reductase activity at 5 weeks in ApFS plants. These results suggest that increases in the contents of photosynthetic intermediates at the early growth stage caused a temporary imbalance in the free-C/free-N ratio and, thus, the feedback inhibition of the expression of genes involved in the Calvin cycle and induction of the expression of those involved in nitrogen metabolism due to supply deficient free amino acids for maintenance of the C/N balance in source leaves of ApFS plants.
[Mh] Termos MeSH primário: Arabidopsis/fisiologia
Carbono/metabolismo
Nitrogênio/metabolismo
Fotossíntese
[Mh] Termos MeSH secundário: Arabidopsis/genética
Arabidopsis/crescimento & desenvolvimento
Proteínas de Arabidopsis/genética
Proteínas de Arabidopsis/metabolismo
Cloroplastos/enzimologia
Frutose-Bifosfatase/genética
Frutose-Bifosfatase/metabolismo
Fenótipo
Monoéster Fosfórico Hidrolases/genética
Monoéster Fosfórico Hidrolases/metabolismo
Plantas Geneticamente Modificadas
Amido/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 7440-44-0 (Carbon); 9005-25-8 (Starch); EC 3.1.3.11 (Fructose-Bisphosphatase); EC 3.1.3.2 (Phosphoric Monoester Hydrolases); EC 3.1.3.37 (sedoheptulose-bisphosphatase); N762921K75 (Nitrogen)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171228
[Lr] Data última revisão:
171228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1007/s10265-017-0950-4


  10 / 15349 MEDLINE  
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[PMID]:27773616
[Au] Autor:Malhotra K; Subramaniyan M; Rawat K; Kalamuddin M; Qureshi MI; Malhotra P; Mohmmed A; Cornish K; Daniell H; Kumar S
[Ad] Endereço:Metabolic Engineering Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India.
[Ti] Título:Compartmentalized Metabolic Engineering for Artemisinin Biosynthesis and Effective Malaria Treatment by Oral Delivery of Plant Cells.
[So] Source:Mol Plant;9(11):1464-1477, 2016 11 07.
[Is] ISSN:1752-9867
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Artemisinin is highly effective against drug-resistant malarial parasites, which affects nearly half of the global population and kills >500 000 people each year. The primary cost of artemisinin is the very expensive process used to extract and purify the drug from Artemisia annua. Elimination of this apparently unnecessary step will make this potent antimalarial drug affordable to the global population living in endemic regions. Here we reported the oral delivery of a non-protein drug artemisinin biosynthesized (∼0.8 mg/g dry weight) at clinically meaningful levels in tobacco by engineering two metabolic pathways targeted to three different cellular compartments (chloroplast, nucleus, and mitochondria). The doubly transgenic lines showed a three-fold enhancement of isopentenyl pyrophosphate, and targeting AACPR, DBR2, and CYP71AV1 to chloroplasts resulted in higher expression and an efficient photo-oxidation of dihydroartemisinic acid to artemisinin. Partially purified extracts from the leaves of transgenic tobacco plants inhibited in vitro growth progression of Plasmodium falciparum-infected red blood cells. Oral feeding of whole intact plant cells bioencapsulating the artemisinin reduced the parasitemia levels in challenged mice in comparison with commercial drug. Such novel synergistic approaches should facilitate low-cost production and delivery of artemisinin and other drugs through metabolic engineering of edible plants.
[Mh] Termos MeSH primário: Artemisininas/metabolismo
Artemisininas/farmacologia
Malária Falciparum/tratamento farmacológico
Engenharia Metabólica
Células Vegetais/metabolismo
[Mh] Termos MeSH secundário: Administração Oral
Animais
Artemisininas/uso terapêutico
Cloroplastos/genética
Camundongos
Plantas Geneticamente Modificadas
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Artemisinins); 9RMU91N5K2 (artemisinine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171213
[Lr] Data última revisão:
171213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE



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