Base de dados : MEDLINE
Pesquisa : A11.284.835 [Categoria DeCS]
Referências encontradas : 27336 [refinar]
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[PMID]:29381737
[Au] Autor:Wang Y; Zhang T; Song X; Zhang J; Dang Z; Pei X; Long Y
[Ad] Endereço:MOA Key Laboratory on Safety Assessment (Molecular) of Agri-GMO, Institute of Biotechnology, Chinese Academy of Agricultural Sciences, Beijing, China.
[Ti] Título:Identification and functional analysis of two alternatively spliced transcripts of ABSCISIC ACID INSENSITIVE3 (ABI3) in linseed flax (Linum usitatissimum L.).
[So] Source:PLoS One;13(1):e0191910, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Alternative splicing is a popular phenomenon in different types of plants. It can produce alternative spliced transcripts that encode proteins with altered functions. Previous studies have shown that one transcription factor, ABSCISIC ACID INSENSITIVE3 (ABI3), which encodes an important component in abscisic acid (ABA) signaling, is subjected to alternative splicing in both mono- and dicotyledons. In the current study, we identified two homologs of ABI3 in the genome of linseed flax. We screened two alternatively spliced flax LuABI3 transcripts, LuABI3-2 and LuABI3-3, and one normal flax LuABI3 transcript, LuABI3-1. Sequence analysis revealed that one of the alternatively spliced transcripts, LuABI3-3, retained a 6 bp intron. RNA accumulation analysis showed that all three transcripts were expressed during seed development, while subcellular localization and transgene experiments showed that LuABI3-3 had no biological function. The two normal transcripts, LuABI3-1 and LuABI3-2, are the important functional isoforms in flax and play significant roles in the ABA regulatory pathway during seed development, germination, and maturation.
[Mh] Termos MeSH primário: Processamento Alternativo
Linho/genética
Genes de Plantas
Proteínas de Plantas/genética
RNA Mensageiro/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Arabidopsis/genética
Germinação/genética
Proteínas de Plantas/química
Plantas Geneticamente Modificadas
Homologia de Sequência de Aminoácidos
Frações Subcelulares/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Plant Proteins); 0 (RNA, Messenger)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180131
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191910


  2 / 27336 MEDLINE  
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[PMID]:29459024
[Au] Autor:Fang P; Yu M; Min W; Wan D; Han S; Shan Y; Wang R; Shi M; Zhang Z; Bo P
[Ad] Endereço:Department of Physiology, Nanjing University of Chinese Medicine Hanlin College, Taizhou, Jiangsu 225300, China; Department of Endocrinology, Clinical Medical College, Yangzhou University, Yangzhou, Jiangsu 225001, China.
[Ti] Título:Effect of baicalin on GLUT4 expression and glucose uptake in myotubes of rats.
[So] Source:Life Sci;196:156-161, 2018 Mar 01.
[Is] ISSN:1879-0631
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:AIMS: Although baicalin could attenuate obesity-induced insulin resistance, the detailed mechanism of baicalin on glucose uptake has not been sufficiently explored as yet. The aim of this study was to survey if baicalin might facilitate glucose uptake and to explore its signal mechanisms in L6 myotubes. MATERIALS AND METHODS: L6 myotubes were treated with 100, 200, 400 µM baicalin for 6 h, 12 h and 24 h in this study. Then 2-NBDG and insulin signal protein levels in myotubes of L6 cells were examined. KEY FINDINGS: We discovered that administration of baicalin enhanced GLUT4, PGC-1α, pP38MAPK, pAKT and pAS160 contents, as well as GLUT4 mRNA and PGC-1α mRNA levels in L6 myotubes. The beneficial metabolic changes elicited by baicalin were abrogated in myotubes of L6 by P38MAPK or AKT inhibitors. SIGNIFICANCE: These results suggest that baicalin promoted glucose uptake in myotubes by differential regulation on P38MAPK and AKT activity. In conclusion, these data provide insight that baicalin is a powerful and promising agent for the treament of hyperglycemia via AKT/AS160/GLUT4 and P38MAPK/PGC1α/GLUT4 pathway.
[Mh] Termos MeSH primário: Flavonoides/farmacologia
Transportador de Glucose Tipo 4/biossíntese
Glucose/metabolismo
Hipoglicemiantes/farmacologia
Fibras Musculares Esqueléticas/metabolismo
[Mh] Termos MeSH secundário: 4-Cloro-7-nitrobenzofurazano/análogos & derivados
4-Cloro-7-nitrobenzofurazano/metabolismo
Animais
Células Cultivadas
Desoxiglucose/análogos & derivados
Desoxiglucose/metabolismo
Insulina/metabolismo
Fibras Musculares Esqueléticas/efeitos dos fármacos
Músculo Esquelético/efeitos dos fármacos
Músculo Esquelético/metabolismo
Proteína Oncogênica v-akt/efeitos dos fármacos
Proteína Oncogênica v-akt/metabolismo
Coativador 1-alfa do Receptor gama Ativado por Proliferador de Peroxissomo/efeitos dos fármacos
Coativador 1-alfa do Receptor gama Ativado por Proliferador de Peroxissomo/metabolismo
Ratos
Transdução de Sinais/efeitos dos fármacos
Frações Subcelulares/efeitos dos fármacos
Frações Subcelulares/metabolismo
Proteínas Quinases p38 Ativadas por Mitógeno/efeitos dos fármacos
Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Flavonoids); 0 (Glucose Transporter Type 4); 0 (Hypoglycemic Agents); 0 (Insulin); 0 (Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha); 0 (Ppargc1a protein, rat); 0 (Slc2a4 protein, rat); 347Q89U4M5 (baicalin); 9G2MP84A8W (Deoxyglucose); EC 2.7.11.1 (Oncogene Protein v-akt); EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases); EQF2794IRE (4-Chloro-7-nitrobenzofurazan); IY9XDZ35W2 (Glucose); JE4F4P486R (2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE


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[PMID]:29278699
[Au] Autor:Yamashita A; Kondo K; Kunishima Y; Iseki S; Kondo T; Ota MS
[Ad] Endereço:Laboratory of Anatomy, Physiology and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Bunkyo-ku, Tokyo, Japan.
[Ti] Título:Postnatal development of bitter taste avoidance behavior in mice is associated with ACTIN-dependent localization of bitter taste receptors to the microvilli of taste cells.
[So] Source:Biochem Biophys Res Commun;495(4):2579-2583, 2018 01 22.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bitter taste avoidance behavior (BAB) plays a fundamental role in the avoidance of toxic substances with a bitter taste. However, the molecular basis underlying the development of BAB is unknown. To study critical developmental events by which taste buds turn into functional organs with BAB, we investigated the early phase development of BAB in postnatal mice in response to bitter-tasting compounds, such as quinine and thiamine. Postnatal mice started to exhibit BAB for thiamine and quinine at postnatal day 5 (PD5) and PD7, respectively. Histological analyses of taste buds revealed the formation of microvilli in the taste pores starting at PD5 and the localization of type 2 taste receptor 119 (TAS2R119) at the microvilli at PD6. Treatment of the tongue epithelium with cytochalasin D (CytD), which disturbs ACTIN polymerization in the microvilli, resulted in the loss of TAS2R119 localization at the microvilli and the loss of BAB for quinine and thiamine. The release of ATP from the circumvallate papillae tissue due to taste stimuli was also declined following CytD treatment. These results suggest that the localization of TAS2R119 at the microvilli of taste pores is critical for the initiation of BAB.
[Mh] Termos MeSH primário: Actinas/metabolismo
Aprendizagem da Esquiva/fisiologia
Microvilosidades/metabolismo
Frações Subcelulares/metabolismo
Papilas Gustativas/fisiologia
Paladar/fisiologia
[Mh] Termos MeSH secundário: Animais
Animais Recém-Nascidos
Feminino
Regulação da Expressão Gênica no Desenvolvimento/fisiologia
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Distribuição Tecidual
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Actins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171227
[St] Status:MEDLINE


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[PMID]:29229392
[Au] Autor:Zhou A; Sun H; Feng S; Zhou M; Gong S; Wang J; Zhang S
[Ad] Endereço:College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China. Electronic address: aiminzhou@neau.edu.cn.
[Ti] Título:A novel cold-regulated gene from Phlox subulata, PsCor413im1, enhances low temperature tolerance in Arabidopsis.
[So] Source:Biochem Biophys Res Commun;495(2):1688-1694, 2018 01 08.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Low temperature stress adversely affects plant growth, development, and crop productivity. Analysis of the function of genes in the response of plants to low temperature stress is essential for understanding the mechanism of chilling and freezing tolerance. In this study, PsCor413im1, a novel cold-regulated gene isolated from Phlox subulata, was transferred to Arabidopsis to investigate its function under low temperature stress. Real-time quantitative PCR analysis revealed that PsCor413im1 expression was induced by cold and abscisic acid. Subcellular localization revealed that PsCor413im1-GFP fusion protein was localized to the periphery of the chloroplast, consistent with the localization of chloroplast inner membrane protein AtCor413im1, indicating that PsCor413im1 is a chloroplast membrane protein. Furthermore, the N-terminal of PsCor413im1 was determined to be necessary for its localization. Compared to the wild-type plants, transgenic plants showed higher germination and survival rates under cold and freezing stress. Moreover, the expression of AtCor15 in transgenic plants was higher than that in the wild-type plants under cold stress. Taken together, our results suggest that the overexpression of PsCor413im1 enhances low temperature tolerance in Arabidopsis.
[Mh] Termos MeSH primário: Aclimatação/genética
Arabidopsis/genética
Ericales/genética
Genes de Plantas
[Mh] Termos MeSH secundário: Aclimatação/fisiologia
Sequência de Aminoácidos
Arabidopsis/crescimento & desenvolvimento
Arabidopsis/fisiologia
Proteínas de Arabidopsis/genética
Proteínas de Arabidopsis/metabolismo
Temperatura Baixa
Ericales/fisiologia
Técnicas de Transferência de Genes
Filogenia
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Plantas Geneticamente Modificadas
Proteínas Recombinantes de Fusão/genética
Proteínas Recombinantes de Fusão/metabolismo
Homologia de Sequência de Aminoácidos
Frações Subcelulares/metabolismo
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Plant Proteins); 0 (Recombinant Fusion Proteins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE


  5 / 27336 MEDLINE  
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[PMID]:29284046
[Au] Autor:Dosemeci A; Burch A; Loo H; Toy D; Tao-Cheng JH
[Ad] Endereço:Laboratory of Neurobiology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, United States of America.
[Ti] Título:IRSp53 accumulates at the postsynaptic density under excitatory conditions.
[So] Source:PLoS One;12(12):e0190250, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:IRSp53 (BAIAP2) is an abundant protein at the postsynaptic density (PSD) that binds to major PSD scaffolds, PSD-95 and Shanks, as well as to F-actin. The distribution of IRSp53 at the PSD in cultured hippocampal neurons was examined under basal and excitatory conditions by immuno-electron microscopy. Under basal conditions, label for IRSp53 is concentrated at the PSD. Upon depolarization by application of a medium containing 90 mM K+, the intensity of IRSp53 label at the PSD increased by 36±7%. Application of NMDA (50 µM) yielded 53±1% increase in the intensity of IRSp53 label at the PSD compared to controls treated with APV, an NMDA antagonist. The accumulation of IRSp53 label upon application of high K+ or NMDA was prominent at the deeper region of the PSD (the PSD pallium, lying 40-120 nm from the postsynaptic plasma membrane). IRSp53 molecules that accumulate at the distal region of the PSD pallium under excitatory conditions are too far from the plasma membrane to fulfill the generally recognized role of the protein as an effector of membrane-bound small GTPases. Instead, these IRSp53 molecules may have a structural role organizing the Shank scaffold and/or linking the PSD to the actin cytoskeleton.
[Mh] Termos MeSH primário: Proteínas do Tecido Nervoso/metabolismo
Densidade Pós-Sináptica/metabolismo
[Mh] Termos MeSH secundário: Animais
Western Blotting
Hipocampo/citologia
Hipocampo/metabolismo
Neurônios/metabolismo
Ratos
Ratos Sprague-Dawley
Frações Subcelulares/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., INTRAMURAL
[Nm] Nome de substância:
0 (BAIAP2 protein, human); 0 (Nerve Tissue Proteins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171229
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190250


  6 / 27336 MEDLINE  
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[PMID]:29253567
[Au] Autor:Vishal SS; Tilwani S; Dalal SN
[Ad] Endereço:KS215, Advanced Centre for Treatment Research and Education in Cancer (ACTREC), Tata Memorial Centre, Kharghar Node, Navi Mumbai 410210, India; Homi Bhabha National Institute, Training School Complex, Anushakti Nagar, Mumbai 400085, India.
[Ti] Título:Plakoglobin localization to the cell border restores desmosome function in cells lacking 14-3-3γ.
[So] Source:Biochem Biophys Res Commun;495(2):1998-2003, 2018 01 08.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Desmosomes are cell-cell adhesion junctions that anchor intermediate filaments. Loss of 14-3-3γ in HCT116 cells led to defects in desmosome assembly due to a decrease in the transport of Plakoglobin (PG) to the cell border thus disrupting desmosome formation. Desmosome formation in cells lacking 14-3-3γ was restored by artificially localizing PG to the cell border by fusing it to EGFP-f (PG-EGFP-f). These results suggest that a major role of 14-3-3γ in desmosome assembly is to transport PG to the cell border leading to the initiation of desmosome formation.
[Mh] Termos MeSH primário: Proteínas 14-3-3/metabolismo
Membrana Celular/metabolismo
Neoplasias Colorretais/metabolismo
Desmossomos/metabolismo
Frações Subcelulares/metabolismo
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Seres Humanos
gama Catenina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (14-3-3 Proteins); 0 (JUP protein, human); 0 (gamma Catenin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE


  7 / 27336 MEDLINE  
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[PMID]:29247649
[Au] Autor:Park KY; Kim WT; Kim EY
[Ad] Endereço:Department of Systems Biology, College of Life Sciences and Biotechnology, Yonsei University, Seoul 03722, South Korea.
[Ti] Título:The proper localization of RESPONSIVE TO DESICCATION 20 in lipid droplets depends on their biogenesis induced by STRESS-RELATED PROTEINS in vegetative tissues.
[So] Source:Biochem Biophys Res Commun;495(2):1885-1889, 2018 01 08.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Arabidopsis LD surface proteins, SRPs are found only in higher plants and are important for LD biogenesis and abiotic stress signaling. However, the cellular mechanism of SRPs is still unclear. To investigate molecular functions of SRPs, we used tobacco transient expression system. Transient expression of SRPs was sufficient and synergistic for LD biogenesis, and SRPs participated in the formation step of LD in tobacco leaves. RESPONSIVE TO DESICCATION 20 (RD20), a known LD-localizing peroxygenase, localized to LD in the presence of an SRP, and its peroxygenase activity correlated with proper localization of RD20 to LD. Our data suggest that Arabidopsis SRPs play roles as positive factors for LD biogenesis to provide a proper localization of LD-localizing proteins in vegetative tissues.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/biossíntese
Arabidopsis/metabolismo
Proteínas de Ligação ao Cálcio/biossíntese
Regulação da Expressão Gênica de Plantas/fisiologia
Proteínas de Choque Térmico/metabolismo
Gotículas Lipídicas/metabolismo
Frações Subcelulares/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Calcium-Binding Proteins); 0 (Heat-Shock Proteins); 0 (RD20 protein, Arabidopsis)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171217
[St] Status:MEDLINE


  8 / 27336 MEDLINE  
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[PMID]:29364608
[Ti] Título:[Not Available.]
[So] Source:Mikrobiologiia;85(5):609-612, 2016 Sep.
[Is] ISSN:0026-3656
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Catecol 1,2-Dioxigenase/metabolismo
Catecóis/metabolismo
Hidroxibenzoatos/farmacologia
Rhodococcus/efeitos dos fármacos
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Proteínas de Bactérias/isolamento & purificação
Catecol 1,2-Dioxigenase/genética
Catecol 1,2-Dioxigenase/isolamento & purificação
Catecóis/química
Ativação Enzimática/efeitos dos fármacos
Ensaios Enzimáticos
Expressão Gênica
Cinética
Rhodococcus/enzimologia
Rhodococcus/genética
Frações Subcelulares/química
Frações Subcelulares/metabolismo
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Catechols); 0 (Hydroxybenzoates); 2ZFW40OJ7U (3-hydroxybenzoic acid); EC 1.13.11.1 (Catechol 1,2-Dioxygenase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE


  9 / 27336 MEDLINE  
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[PMID]:29219067
[Au] Autor:Fan Y; Tang X; Hu X; Wu W; Ping Q
[Ad] Endereço:School of Mathematics, Liaoning University, Shenyang, 110036, China.
[Ti] Título:Prediction of essential proteins based on subcellular localization and gene expression correlation.
[So] Source:BMC Bioinformatics;18(Suppl 13):470, 2017 Dec 01.
[Is] ISSN:1471-2105
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Essential proteins are indispensable to the survival and development process of living organisms. To understand the functional mechanisms of essential proteins, which can be applied to the analysis of disease and design of drugs, it is important to identify essential proteins from a set of proteins first. As traditional experimental methods designed to test out essential proteins are usually expensive and laborious, computational methods, which utilize biological and topological features of proteins, have attracted more attention in recent years. Protein-protein interaction networks, together with other biological data, have been explored to improve the performance of essential protein prediction. RESULTS: The proposed method SCP is evaluated on Saccharomyces cerevisiae datasets and compared with five other methods. The results show that our method SCP outperforms the other five methods in terms of accuracy of essential protein prediction. CONCLUSIONS: In this paper, we propose a novel algorithm named SCP, which combines the ranking by a modified PageRank algorithm based on subcellular compartments information, with the ranking by Pearson correlation coefficient (PCC) calculated from gene expression data. Experiments show that subcellular localization information is promising in boosting essential protein prediction.
[Mh] Termos MeSH primário: Algoritmos
Biologia Computacional/métodos
Regulação Fúngica da Expressão Gênica
Genes Essenciais
Mapas de Interação de Proteínas
Proteínas de Saccharomyces cerevisiae/metabolismo
Saccharomyces cerevisiae/metabolismo
[Mh] Termos MeSH secundário: Saccharomyces cerevisiae/genética
Proteínas de Saccharomyces cerevisiae/genética
Frações Subcelulares
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Saccharomyces cerevisiae Proteins)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180130
[Lr] Data última revisão:
180130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171209
[St] Status:MEDLINE
[do] DOI:10.1186/s12859-017-1876-5


  10 / 27336 MEDLINE  
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[PMID]:29180010
[Au] Autor:Maeda A; Uchida M; Nishikawa S; Nishino T; Konishi H
[Ad] Endereço:Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, Shobara, Hiroshima 727-0023, Japan.
[Ti] Título:Role of N-myristoylation in stability and subcellular localization of the CLPABP protein.
[So] Source:Biochem Biophys Res Commun;495(1):1249-1256, 2018 01 01.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cardiolipin and phosphatidic acid-binding protein (CLPABP) controls the stability of the mRNA harboring an AU-rich element (ARE) in the 3' UTR with the help of the RNA stabilizer, human antigen R (HuR). Although CLPABP is localized on the mitochondrial surface as a large protein-RNA complex, its precise role is not yet known. Recently, CLPABP was identified as an N-myristoylated protein. Here, we demonstrate the effects of N-myristoylation on the functions of CLPABP. In the present study, compared to the wild-type protein that possessed the "MG" motif at the N-terminus for N-myristoylation, the mutant CLPABP protein that lacked N-myristoylation modification site was unstable. Furthermore, the expression of the G/A mutant of CLPABP, which lacked N-myristoylation site, induced morphological alterations in mitochondria. Because pleckstrin homology domain-deleted mutant, which was fused with the N-myristoylation site derived from intact CLPABP, could not colocalize with mitochondria, N-myristoylation of CLPABP was predicted to affect its stability onto the mitochondrial membrane rather than its subcellular localization.
[Mh] Termos MeSH primário: Metabolismo dos Lipídeos/fisiologia
Proteínas Ligadas a Lipídeos/metabolismo
Ácido Mirístico/metabolismo
Prenilação de Proteína/fisiologia
Frações Subcelulares/metabolismo
[Mh] Termos MeSH secundário: Animais
Células COS
Cercopithecus aethiops
Células HEK293
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Lipid-Linked Proteins); 0 (PLEKHN1 protein, human); 0I3V7S25AW (Myristic Acid)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180105
[Lr] Data última revisão:
180105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE



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BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde