Base de dados : MEDLINE
Pesquisa : A11.284.917 [Categoria DeCS]
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[PMID]:28745103
[Au] Autor:Divilov K; Wiesner-Hanks T; Barba P; Cadle-Davidson L; Reisch BI
[Ad] Endereço:First, second, and third authors: Plant Breeding and Genetics Section, School of Integrative Plant Science, Cornell University, Ithaca, NY; fourth author: United States Department of Agriculture-Agricultural Research Service Grape Genetics Research Unit, Geneva, NY; and fifth author: Horticulture Se
[Ti] Título:Computer Vision for High-Throughput Quantitative Phenotyping: A Case Study of Grapevine Downy Mildew Sporulation and Leaf Trichomes.
[So] Source:Phytopathology;107(12):1549-1555, 2017 12.
[Is] ISSN:0031-949X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Quantitative phenotyping of downy mildew sporulation is frequently used in plant breeding and genetic studies, as well as in studies focused on pathogen biology such as chemical efficacy trials. In these scenarios, phenotyping a large number of genotypes or treatments can be advantageous but is often limited by time and cost. We present a novel computational pipeline dedicated to estimating the percent area of downy mildew sporulation from images of inoculated grapevine leaf discs in a manner that is time and cost efficient. The pipeline was tested on images from leaf disc assay experiments involving two F grapevine families, one that had glabrous leaves (Vitis rupestris B38 × 'Horizon' [RH]) and another that had leaf trichomes (Horizon × V. cinerea B9 [HC]). Correlations between computer vision and manual visual ratings reached 0.89 in the RH family and 0.43 in the HC family. Additionally, we were able to use the computer vision system prior to sporulation to measure the percent leaf trichome area. We estimate that an experienced rater scoring sporulation would spend at least 90% less time using the computer vision system compared with the manual visual method. This will allow more treatments to be phenotyped in order to better understand the genetic architecture of downy mildew resistance and of leaf trichome density. We anticipate that this computer vision system will find applications in other pathosystems or traits where responses can be imaged with sufficient contrast from the background.
[Mh] Termos MeSH primário: Peronospora/citologia
Doenças das Plantas/microbiologia
Vitis/microbiologia
[Mh] Termos MeSH secundário: Genótipo
Processamento de Imagem Assistida por Computador
Peronospora/isolamento & purificação
Fenótipo
Folhas de Planta/microbiologia
Smartphone
Esporos/citologia
Esporos/isolamento & purificação
Tricomas/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.1094/PHYTO-04-17-0137-R


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[PMID]:27770776
[Au] Autor:Jooste M; Dreyer LL; Oberlander KC
[Ad] Endereço:Department of Botany and Zoology, University of Stellenbosch, Private Bag X1, Matieland, 7602, South Africa. mich.jooste.m@gmail.com.
[Ti] Título:The phylogenetic significance of leaf anatomical traits of southern African Oxalis.
[So] Source:BMC Evol Biol;16(1):225, 2016 10 22.
[Is] ISSN:1471-2148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The southern African Oxalis radiation is extremely morphologically variable. Despite recent progress in the phylogenetics of the genus, there are few morphological synapomorphies supporting DNA-based clades. Leaflet anatomy can provide an understudied and potentially valuable source of information on the evolutionary history and systematics of this lineage. Fifty-nine leaflet anatomical traits of 109 southern African Oxalis species were assessed in search of phylogenetically significant characters that delineate clades. RESULTS: A combination of 6 leaflet anatomical traits (stomatal position, adaxial epidermal cells, abaxial epidermal cells, mesophyll, sheath around vascular tissue, degree of leaflet conduplication) clearly support various clades defined by previous DNA-based phylogenetic work. Other, mostly continuous leaflet anatomical traits were highly variable and showed less phylogenetic pattern. CONCLUSIONS: Major and unexpected findings include the transition from ancestral hypostomatic leaflets to adaxially-located stomata in the vast majority of southern African Oxalis, the loss of semi-swollen AB epidermal cells and the gain of swollen adaxial and abaxial epidermal cells in selected clades, and multiple changes from ancestral bifacial mesophyll to isobilateral or homogenous mesophyll types. The information gathered in this study will aid in the taxonomic revision of this speciose member of the Greater Cape Floristic Region and provide a basis for future hypotheses regarding its radiation.
[Mh] Termos MeSH primário: Oxalidaceae/anatomia & histologia
Filogenia
Folhas de Planta/anatomia & histologia
[Mh] Termos MeSH secundário: Evolução Biológica
Duplicação Gênica
Células do Mesofilo/citologia
Oxalidaceae/genética
Fenótipo
Folhas de Planta/citologia
Folhas de Planta/genética
Estômatos de Plantas/citologia
Feixe Vascular de Plantas/citologia
Característica Quantitativa Herdável
Tricomas/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1708
[Cu] Atualização por classe:180128
[Lr] Data última revisão:
180128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


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[PMID]:28460081
[Au] Autor:Lihavainen J; Ahonen V; Keski-Saari S; Sõber A; Oksanen E; Keinänen M
[Ad] Endereço:Department of Environmental and Biological Sciences, University of Eastern Finland, PO Box 111, 80101 Joensuu, Finland.
[Ti] Título:Low vapor pressure deficit reduces glandular trichome density and modifies the chemical composition of cuticular waxes in silver birch leaves.
[So] Source:Tree Physiol;37(9):1166-1181, 2017 Sep 01.
[Is] ISSN:1758-4469
[Cp] País de publicação:Canada
[La] Idioma:eng
[Ab] Resumo:Cuticular wax layer is the first barrier against the outside environment and the first defense encountered by herbivores and pathogens. The effects of environmental factors on cuticular chemistry, and on the formation of glandular trichomes that account for the storage and secretion of lipophilic compounds to the leaf surface are poorly understood. Low vapor pressure deficit (VPD) has shown to reduce the nitrogen (N) status of plants. Thus, we studied the effects of elevated air humidity, indicated as VPD, and the effect of N fertilization on cuticular waxes and glandular trichome density in silver birch (Betula pendula Roth). Experiments were carried out in growth chambers with juvenile plants and in a long-term field experiment with older trees. Low VPD reduced the glandular trichome density in both experiments, in chamber and in field. The contents of the major triterpenoid and flavonoid aglycones correlated positively with glandular trichome density, which supports the role of trichomes in the exudation of secondary compounds to the leaf surface. A closer examination of the cuticular wax chemistry in the chamber experiment revealed that low VPD and N supply affected the composition of cuticular waxes, but not the total wax content. The deposition of different wax compounds followed a co-ordinated pattern in birch leaves, but different compound groups varied in their responses to N fertilization and low VPD. Low VPD reduced the hydrophobicity of cuticular waxes, as demonstrated by lower alkane content and less hydrophobic flavonoid profile in low VPD than in high VPD. Reduced hydrophobicity of the wax layer is presumed to increase leaf wettability. Together with reduced trichome density in low VPD it may enhance the susceptibility of trees to fungal pathogens and herbivores. High N supply under low VPD reduced the effect of low VPD on the cuticular wax composition. Total fatty acid content and the expression of ß-amyrin synthase were lower under high N supply than under moderate N supply irrespective of VPD treatment. Nitrogen availability and decreasing VPD will modify leaf surface properties in silver birch and thereby affect tree defence against abiotic and biotic stress factors that emerge under climate change.
[Mh] Termos MeSH primário: Betula/fisiologia
Folhas de Planta/química
Tricomas/fisiologia
Pressão de Vapor
Ceras/química
[Mh] Termos MeSH secundário: Mudança Climática
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Waxes)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180115
[Lr] Data última revisão:
180115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE
[do] DOI:10.1093/treephys/tpx045


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[PMID]:28806605
[Au] Autor:Colombo PS; Flamini G; Rodondi G; Giuliani C; Santagostini L; Fico G
[Ad] Endereço:Dipartimento di Scienze Farmaceutiche, Università degli Studi di Milano, Via Mangiagalli 25, 20133 Milano, Italy; Orto Botanico G.E. Ghirardi, Dipartimento di Scienze Farmaceutiche, Via Religione 25, 25088, Toscolano Maderno, Brescia, Italy. Electronic address: pasico19@virgilio.it.
[Ti] Título:Phytochemistry of European Primula species.
[So] Source:Phytochemistry;143:132-144, 2017 Nov.
[Is] ISSN:1873-3700
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The genus Primula is the largest among the Primulaceae and is widespread mainly in the cold and temperate regions of the Northern Hemisphere. Since the beginning of the Twentieth century, several studies on the phytochemical composition of different species of Primula have been carried out. The main constituents examined were tissue and epicuticular flavonoids and saponins, which are of therapeutic significance. Only in recent years studies of the volatiles emitted by leaves and flowers have been carried out as well, but they are restricted to a small number of species. Only a few authors have documented the morphology and function of glandular trichomes in relation to the production of flavonoids and volatile organic compounds (VOCs). The use of Primula in folk medicine is described in the literature. Investigation of the biological and pharmacological activities of Primula are reported. This study aims at providing a collection of publications on the genus Primula along with a critical revision of literature data. It focuses on the possible taxonomic significance of the secondary metabolites and on their ecological role as attractors for pollinators and deterrents against herbivores and parasites, in order to build the base for further studies.
[Mh] Termos MeSH primário: Flavonoides/isolamento & purificação
Primula/química
Primulaceae/química
[Mh] Termos MeSH secundário: Europa (Continente)
Flavonoides/química
Flores
Estrutura Molecular
Folhas de Planta/química
Saponinas/química
Saponinas/isolamento & purificação
Tricomas/metabolismo
Compostos Orgânicos Voláteis/química
Compostos Orgânicos Voláteis/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Flavonoids); 0 (Saponins); 0 (Volatile Organic Compounds)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170815
[St] Status:MEDLINE


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[PMID]:28690154
[Au] Autor:Yin X; Cui Y; Wang M; Xia X
[Ad] Endereço:Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, People's Republic of China.
[Ti] Título:Overexpression of a novel MYB-related transcription factor, OsMYBR1, confers improved drought tolerance and decreased ABA sensitivity in rice.
[So] Source:Biochem Biophys Res Commun;490(4):1355-1361, 2017 Sep 02.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The MYB proteins play important roles in regulating plant responses to environmental stresses. We cloned and functionally characterized a novel MYB-related gene, OsMYBR1, from rice. Our microarray and qRT-PCR analyses showed that its expression was induced by drought and cold in different tissues at various developmental stages. This gene encodes a putative MYB-related protein of 463 amino acid residues. Compared with wild-type (WT) plants, transgenic plants over-expressing OsMYBR1 exhibited much greater tolerance to drought stress and decreased sensitivity to abscisic acid (ABA). Under drought treatment, levels of free proline and soluble sugar were higher in transgenic plants than in the WT. Furthermore, transcriptional expression of four stress-related genes -- OsP5CS1, OsProt, OsLEA3, and OsRab16 -- was significantly increased in transgenic plants under drought stressed conditions and ABA. Our results provide evidence that OsMYBR1 is involved in mediating plant responses to ABA and drought.
[Mh] Termos MeSH primário: Ácido Abscísico/metabolismo
Adaptação Fisiológica/genética
Regulação da Expressão Gênica de Plantas
Oryza/genética
Plantas Geneticamente Modificadas
Fatores de Transcrição/genética
[Mh] Termos MeSH secundário: Ácido Abscísico/farmacologia
Temperatura Baixa
Secas
Perfilação da Expressão Gênica
Regulação da Expressão Gênica no Desenvolvimento
Glutamato-5-Semialdeído Desidrogenase/genética
Glutamato-5-Semialdeído Desidrogenase/metabolismo
Proteínas de Choque Térmico/genética
Proteínas de Choque Térmico/metabolismo
Análise em Microsséries
Oryza/efeitos dos fármacos
Oryza/crescimento & desenvolvimento
Oryza/metabolismo
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Transdução de Sinais
Estresse Fisiológico
Fatores de Transcrição/metabolismo
Tricomas/efeitos dos fármacos
Tricomas/genética
Tricomas/crescimento & desenvolvimento
Tricomas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Heat-Shock Proteins); 0 (Plant Proteins); 0 (Transcription Factors); 0 (late embryogenesis abundant protein, plant); 128964-27-2 (Rab16A protein, Oryza sativa); 72S9A8J5GW (Abscisic Acid); EC 1.2.1.41 (Glutamate-5-Semialdehyde Dehydrogenase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170711
[St] Status:MEDLINE


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[PMID]:28644898
[Au] Autor:Ren H; Dang X; Cai X; Yu P; Li Y; Zhang S; Liu M; Chen B; Lin D
[Ad] Endereço:Basic Forestry and Proteomics Research Center, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, China.
[Ti] Título:Spatio-temporal orientation of microtubules controls conical cell shape in Arabidopsis thaliana petals.
[So] Source:PLoS Genet;13(6):e1006851, 2017 Jun.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The physiological functions of epidermal cells are largely determined by their diverse morphologies. Most flowering plants have special conical-shaped petal epidermal cells that are thought to influence light capture and reflectance, and provide pollinator grips, but the molecular mechanisms controlling conical cell shape remain largely unknown. Here, we developed a live-confocal imaging approach to quantify geometric parameters of conical cells in Arabidopsis thaliana (A. thaliana). Through genetic screens, we identified katanin (KTN1) mutants showing a phenotype of decreased tip sharpening of conical cells. Furthermore, we demonstrated that SPIKE1 and Rho of Plants (ROP) GTPases were required for the final shape formation of conical cells, as KTN1 does. Live-cell imaging showed that wild-type cells exhibited random orientation of cortical microtubule arrays at early developmental stages but displayed a well-ordered circumferential orientation of microtubule arrays at later stages. By contrast, loss of KTN1 prevented random microtubule networks from shifting into well-ordered arrays. We further showed that the filamentous actin cap, which is a typical feature of several plant epidermal cell types including root hairs and leaf trichomes, was not observed in the growth apexes of conical cells during cell development. Moreover, our genetic and pharmacological data suggested that microtubules but not actin are required for conical cell shaping. Together, our results provide a novel imaging approach for studying petal conical cell morphogenesis and suggest that the spatio-temporal organization of microtubule arrays plays crucial roles in controlling conical cell shape.
[Mh] Termos MeSH primário: Adenosina Trifosfatases/genética
Proteínas de Arabidopsis/genética
Forma Celular/genética
Flores/genética
[Mh] Termos MeSH secundário: Citoesqueleto de Actina/genética
Arabidopsis/genética
Arabidopsis/crescimento & desenvolvimento
Epiderme/citologia
Epiderme/crescimento & desenvolvimento
Flores/crescimento & desenvolvimento
Flores/ultraestrutura
Proteínas de Ligação ao GTP/genética
Katanina
Microtúbulos/genética
Microtúbulos/ultraestrutura
Proteínas Mutantes/genética
Folhas de Planta/citologia
Folhas de Planta/genética
Tricomas/genética
Tricomas/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Mutant Proteins); 0 (SPIKE1 protein, Arabidopsis); EC 3.6.1.- (Adenosine Triphosphatases); EC 3.6.1.- (GTP-Binding Proteins); EC 3.6.1.- (ROP1 protein, Arabidopsis); EC 3.6.4.3 (KTN1 protein, Arabidopsis); EC 3.6.4.3 (Katanin)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170624
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1006851


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[PMID]:28644769
[Au] Autor:Tominaga-Wada R; Kurata T; Wada T
[Ad] Endereço:a Graduate School of Biosphere Sciences , Hiroshima University , Higashi-Hiroshima , Japan.
[Ti] Título:Localization of the CAPRICE-ENHANCER OF TRY AND CPC1 chimera protein in Arabidopsis root epidermis.
[So] Source:Biosci Biotechnol Biochem;81(9):1762-1767, 2017 Sep.
[Is] ISSN:1347-6947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The CAPRICE (CPC) encodes an R3-type MYB transcription factor, which promotes root-hair differentiation. Previously, we showed that the CPC protein moves from the non-hair cell to the neighboring cell and induces root-hair differentiation in Arabidopsis. In addition, we proposed two cell-to-cell movement signal sequences, S1 and S2, in CPC. However, an S1:2xGFP:S2 chimera protein did not move between root epidermal cells. Here, we show that the S1 and S2 sequences do not confer cell-to-cell movement or nuclear localization ability to a GFP protein. The ENHANCER OF TRY AND CPC1 (ETC1) gene encodes the CPC homolog R3 MYB; this protein does not possess cell-to-cell movement ability or the S1 sequence. To elucidate whether the S1 sequence can induce cell-to-cell movement ability in ETC1, CPCp:S1:ETC1:2xGFP was constructed and introduced into Arabidopsis. Our results indicate that the addition of the S1 sequence was not sufficient for ETC1 to acquire cell-to-cell movement ability.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/metabolismo
Arabidopsis/metabolismo
Proteínas de Ligação a DNA/metabolismo
Epiderme Vegetal/metabolismo
Raízes de Plantas/metabolismo
Proteínas Proto-Oncogênicas c-myb/metabolismo
Proteínas Recombinantes de Fusão/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Arabidopsis/citologia
Proteínas de Arabidopsis/química
Proteínas de Arabidopsis/genética
Núcleo Celular/metabolismo
Proteínas de Ligação a DNA/genética
Fenótipo
Transporte Proteico
Proteínas Proto-Oncogênicas c-myb/química
Proteínas Proto-Oncogênicas c-myb/genética
Proteínas Recombinantes de Fusão/genética
Tricomas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (CPC protein, Arabidopsis); 0 (DNA-Binding Proteins); 0 (Enhancer of Try and CPC1, Arabidopsis); 0 (Proto-Oncogene Proteins c-myb); 0 (Recombinant Fusion Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170624
[St] Status:MEDLINE
[do] DOI:10.1080/09168451.2017.1343120


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[PMID]:28274946
[Au] Autor:Mustafa A; Ensikat HJ; Weigend M
[Ad] Endereço:Nees-Institut für Biodiversität der Pflanzen, Rheinische Friedrich-Wilhelms-Universität Bonn, Meckenheimer Allee 170, D-53115 Bonn, Germany.
[Ti] Título:Ontogeny and the process of biomineralization in the trichomes of Loasaceae.
[So] Source:Am J Bot;104(3):367-378, 2017 Mar.
[Is] ISSN:1537-2197
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PREMISE OF THE STUDY: South American Loasaceae have a morphologically complex trichome cover, which is characterized by multiple biomineralization. The current study investigates the ontogeny of these complex trichomes and the process of their biomineralization, since both are very poorly understood. METHODS: The development of stinging trichomes on various parts of the plants and the process of mineralization were studied using scanning electron microscopy (SEM) and energy-dispersive x-ray spectroscopy (EDX). KEY RESULTS: Trichomes are initiated very early in organ development and the different trichome types begin developing their distinctive morphology at a very early developmental stage. Biomineralization in the stinging trichomes starts with the deposition of silica or calcium phosphate in the apex and then proceeds basipetally, with a more irregular, subsimultaneous mineralization of the base and the shaft. Mineralization of the scabrid-glochidiate trichomes starts on the surface processes and in the apex (silica, calcium phosphate), with a subsequent mineralization of the shaft with calcium carbonate. CONCLUSION: Mineralized trichomes in Loasaceae provide an excellent model for the study of biomineralization. The overall sequence of mineralization is typically from distal and peripheral to proximal and central. Typically, three biominerals-silica, calcium carbonate, and calcium phosphate-are differentially and sequentially deposited in different parts of each unicellular stinging trichome.
[Mh] Termos MeSH primário: Ontologias Biológicas
Magnoliopsida/metabolismo
Minerais/metabolismo
Tricomas/metabolismo
[Mh] Termos MeSH secundário: Fosfatos de Cálcio/metabolismo
Magnoliopsida/crescimento & desenvolvimento
Magnoliopsida/ultraestrutura
Microscopia Eletrônica de Varredura
Modelos Biológicos
Folhas de Planta/crescimento & desenvolvimento
Folhas de Planta/metabolismo
Folhas de Planta/ultraestrutura
Especificidade da Espécie
Tricomas/crescimento & desenvolvimento
Tricomas/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Calcium Phosphates); 0 (Minerals); 97Z1WI3NDX (calcium phosphate)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170310
[St] Status:MEDLINE
[do] DOI:10.3732/ajb.1600417


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[PMID]:28258381
[Au] Autor:Kawakatsu Y; Nakayama H; Kaminoyama K; Igarashi K; Yasugi M; Kudoh H; Nagano AJ; Yano K; Kubo N; Kimura S
[Ad] Endereço:Department of Bioresource and Environmental Sciences, Faculty of Life Sciences, Kyoto Sangyo University, Kamigamo-motoyama, Kita-ku, Kyoto, 603-8555, Japan.
[Ti] Título:A GLABRA1 ortholog on LG A9 controls trichome number in the Japanese leafy vegetables Mizuna and Mibuna (Brassica rapa L. subsp. nipposinica L. H. Bailey): evidence from QTL analysis.
[So] Source:J Plant Res;130(3):539-550, 2017 May.
[Is] ISSN:1618-0860
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Brassica rapa show a wide range of morphological variations. In particular, the leaf morphologies of the Japanese traditional leafy vegetables Mizuna and Mibuna (Brassica rapa L. subsp. nipposinica L. H. Bailey) are distinctly different, even though they are closely related cultivars that are easy to cross. In addition to the differences in the gross morphology of leaves, some cultivars of Mibuna (Kyo-nishiki) have many trichomes on its leaves, whereas Mizuna (Kyo-mizore) does not. To identify the genes responsible for the different number of trichomes, we performed a quantitative trait loci (QTL) analysis of Mizuna and Mibuna. To construct linkage maps for these cultivars, we used RNA-seq data to develop cleaved amplified polymorphic sequence (CAPS) markers. We also performed a restriction site-associated DNA sequencing (RAD-seq) analysis to detect single-nucleotide polymorphisms (SNPs). Two QTL analyses were performed in different years, and both analyses indicated that the largest effect was found on LG A9. Expression analyses showed that a gene homologous to GLABRA1 (GL1), a transcription factor implicated in trichome development in Arabidopsis thaliana, and the sequences 3'-flanking (downstream) of BrGL1, differed considerably between Mizuna (Kyo-mizore) and Mibuna (Kyo-nishiki). These results indicate that BrGL1 on LG A9 is one of the candidate genes responsible for the difference in trichome number between Mizuna and Mibuna. Detecting genes that are responsible for morphological variations allows us to better understand the breeding history of Mizuna and Mibuna.
[Mh] Termos MeSH primário: Brassica rapa/genética
Locos de Características Quantitativas/genética
Tricomas/genética
Verduras/genética
[Mh] Termos MeSH secundário: Arabidopsis/genética
Proteínas de Arabidopsis/genética
Sequência de Bases
Brassica rapa/anatomia & histologia
Brassica rapa/classificação
Cruzamento
Mapeamento Cromossômico
Proteínas de Ligação a DNA/genética
Regulação da Expressão Gênica de Plantas
Genes de Plantas/genética
Ligação Genética
Marcadores Genéticos
Técnicas de Genotipagem
Japão
Fenótipo
Filogenia
Folhas de Planta/anatomia & histologia
Folhas de Planta/genética
Polimorfismo de Nucleotídeo Único/genética
Alinhamento de Sequência
Análise de Sequência de DNA
Fatores de Transcrição
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (DNA-Binding Proteins); 0 (Genetic Markers); 0 (Transcription Factors); 144417-31-2 (GL1 protein, Arabidopsis)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170305
[St] Status:MEDLINE
[do] DOI:10.1007/s10265-017-0917-5


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[PMID]:28250069
[Au] Autor:Laterre R; Pottier M; Remacle C; Boutry M
[Ad] Endereço:Institut des Sciences de la Vie, University of Louvain, 1348 Louvain-la-Neuve, Belgium (R.L., M.P., M.B.); and.
[Ti] Título:Photosynthetic Trichomes Contain a Specific Rubisco with a Modified pH-Dependent Activity.
[So] Source:Plant Physiol;173(4):2110-2120, 2017 Apr.
[Is] ISSN:1532-2548
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ribulose-1,5-biphosphate carboxylase/oxygenase (Rubisco) is the most abundant enzyme in plants and is responsible for CO fixation during photosynthesis. This enzyme is assembled from eight large subunits (RbcL) encoded by a single chloroplast gene and eight small subunits (RbcS) encoded by a nuclear gene family. Rubisco is primarily found in the chloroplasts of mesophyll (C3 plants), bundle-sheath (C4 plants), and guard cells. In certain species, photosynthesis also takes place in the secretory cells of glandular trichomes, which are epidermal outgrowths (hairs) involved in the secretion of specialized metabolites. However, photosynthesis and, in particular, Rubisco have not been characterized in trichomes. Here, we show that tobacco ( ) trichomes contain a specific Rubisco small subunit, NtRbcS-T, which belongs to an uncharacterized phylogenetic cluster (T). This cluster contains RbcS from at least 33 species, including monocots, many of which are known to possess glandular trichomes. Cluster T is distinct from the cluster M, which includes the abundant, functionally characterized RbcS isoforms expressed in mesophyll or bundle-sheath cells. Expression of NtRbcS-T in and purification of the full Rubisco complex showed that this isoform conferred higher and values as well as higher acidic pH-dependent activity than NtRbcS-M, an isoform expressed in the mesophyll. This observation was confirmed with trichome extracts. These data show that an ancient divergence allowed for the emergence of a so-far-uncharacterized RbcS cluster. We propose that secretory trichomes have a particular Rubisco uniquely adapted to secretory cells where CO is released by the active specialized metabolism.
[Mh] Termos MeSH primário: Fotossíntese
Proteínas de Plantas/metabolismo
Ribulose-Bifosfato Carboxilase/metabolismo
Tricomas/enzimologia
[Mh] Termos MeSH secundário: Dióxido de Carbono/metabolismo
Chlamydomonas reinhardtii/enzimologia
Chlamydomonas reinhardtii/genética
Chlamydomonas reinhardtii/metabolismo
Eletroforese em Gel Bidimensional
Regulação da Expressão Gênica de Plantas
Concentração de Íons de Hidrogênio
Cinética
Espectrometria de Massas
Filogenia
Folhas de Planta/genética
Folhas de Planta/metabolismo
Proteínas de Plantas/genética
Subunidades Proteicas/classificação
Subunidades Proteicas/genética
Subunidades Proteicas/metabolismo
Proteômica/métodos
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Ribulose-Bifosfato Carboxilase/classificação
Ribulose-Bifosfato Carboxilase/genética
Tabaco/enzimologia
Tabaco/genética
Tabaco/metabolismo
Tricomas/genética
Tricomas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); 0 (Protein Subunits); 142M471B3J (Carbon Dioxide); EC 4.1.1.39 (Ribulose-Bisphosphate Carboxylase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170303
[St] Status:MEDLINE
[do] DOI:10.1104/pp.17.00062



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