Base de dados : MEDLINE
Pesquisa : A11.329 [Categoria DeCS]
Referências encontradas : 2679 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 268 ir para página                         

  1 / 2679 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27770633
[Au] Autor:Lee NM; Erisken C; Iskratsch T; Sheetz M; Levine WN; Lu HH
[Ad] Endereço:Biomaterials and Interface Tissue Engineering Laboratory, Department of Biomedical Engineering, Columbia University, New York, NY 10027, United States.
[Ti] Título:Polymer fiber-based models of connective tissue repair and healing.
[So] Source:Biomaterials;112:303-312, 2017 01.
[Is] ISSN:1878-5905
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Physiologically relevant models of wound healing are essential for understanding the biology of connective tissue repair and healing. They can also be used to identify key cellular processes and matrix characteristics critical for the design of soft tissue grafts. Modeling the various stages of repair post tendon injury, polymer meshes of varying fiber diameter (nano-1 (390 nm) < nano-2 (740 nm) < micro (1420 nm)) were produced. Alignment was also introduced in the nano-2 group to model matrix undergoing biological healing rather than scar formation. The response of human tendon fibroblasts on these model substrates were evaluated over time as a function of fiber diameter and alignment. It was observed that the repair models of unaligned nanoscale fibers enhanced cell growth and collagen synthesis, while these outcomes were significantly reduced in the mature repair model consisting of unaligned micron-sized fibers. Organization of paxillin and actin on unaligned meshes was enhanced on micro- compared to nano-sized fibers, while the expression and activity of RhoA and Rac1 were greater on nanofibers. In contrast, aligned nanofibers promoted early cell organization, while reducing excessive cell growth and collagen production in the long term. These results show that the early-stage repair model of unaligned nanoscale fibers elicits a response characteristic of the proliferative phase of wound repair, while the more mature model consisting of unaligned micron-sized fibers is more representative of the remodeling phase by supporting cell organization while suppressing growth and biosynthesis. Interestingly, introduction of fiber alignment in the nanofiber model alters fibroblast response from repair to healing, implicating matrix alignment as a critical design factor for circumventing scar formation and promoting biological healing of soft tissue injuries.
[Mh] Termos MeSH primário: Células do Tecido Conjuntivo/citologia
Células do Tecido Conjuntivo/fisiologia
Nanofibras/química
Polímeros/química
Traumatismos dos Tendões/fisiopatologia
Tecidos Suporte
Cicatrização/fisiologia
[Mh] Termos MeSH secundário: Idoso
Células Cultivadas
Tecido Conjuntivo/fisiologia
Feminino
Fibroblastos/citologia
Fibroblastos/fisiologia
Seres Humanos
Masculino
Meia-Idade
Nanofibras/ultraestrutura
Traumatismos dos Tendões/patologia
Engenharia Tecidual/instrumentação
Engenharia Tecidual/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Polymers)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161023
[St] Status:MEDLINE


  2 / 2679 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28634338
[Au] Autor:Singh LR; Avula SR; Raj S; Srivastava A; Palnati GR; Tripathi CKM; Pasupuleti M; Sashidhara KV
[Ad] Endereço:Division of Medicinal and Process Chemistry, CSIR-Central Drug Research Institute, Lucknow, India.
[Ti] Título:Coumarin-benzimidazole hybrids as a potent antimicrobial agent: synthesis and biological elevation.
[So] Source:J Antibiot (Tokyo);70(9):954-961, 2017 Aug.
[Is] ISSN:0021-8820
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Molecular hybridization approach is an emerging tool in drug discovery for designing new pharmacophores with biological activity. A novel, new series of coumarin-benzimidazole hybrids were designed, synthesized and evaluated for their broad spectrum antimicrobial activity. Among all the synthesized molecules, compound (E)-3-(2-1H-benzo[d]imidazol-1-yl)-1-((4-chlorobenzyl)oxy)imino)ethyl)-2H-chromen-2-one showed the most promising broad spectrum antibacterial activity against Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis and Proteus vulgaris. In addition, it has showed no cytotoxicity and hemolysis at 10 times the MIC concentration. SAR studies indicate that position of the chlorine atom in the hybrid critically determines the antibacterial activity.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Anti-Infecciosos/farmacologia
Benzimidazóis/farmacologia
Cumarínicos/farmacologia
Desenho de Drogas
Farmacorresistência Bacteriana Múltipla
Modelos Moleculares
[Mh] Termos MeSH secundário: Animais
Antibacterianos/efeitos adversos
Antibacterianos/síntese química
Antibacterianos/química
Anti-Infecciosos/efeitos adversos
Anti-Infecciosos/síntese química
Anti-Infecciosos/química
Bacillus subtilis/efeitos dos fármacos
Bacillus subtilis/crescimento & desenvolvimento
Benzimidazóis/efeitos adversos
Benzimidazóis/síntese química
Benzimidazóis/química
Linhagem Celular
Sobrevivência Celular/efeitos dos fármacos
Células do Tecido Conjuntivo/citologia
Células do Tecido Conjuntivo/efeitos dos fármacos
Cumarínicos/efeitos adversos
Cumarínicos/síntese química
Cumarínicos/química
Hemólise/efeitos dos fármacos
Seres Humanos
Hidrocarbonetos Clorados/efeitos adversos
Hidrocarbonetos Clorados/síntese química
Hidrocarbonetos Clorados/química
Hidrocarbonetos Clorados/farmacologia
Camundongos
Testes de Sensibilidade Microbiana
Estrutura Molecular
Proteus vulgaris/efeitos dos fármacos
Proteus vulgaris/crescimento & desenvolvimento
Pseudomonas aeruginosa/efeitos dos fármacos
Pseudomonas aeruginosa/crescimento & desenvolvimento
Staphylococcus aureus/efeitos dos fármacos
Staphylococcus aureus/crescimento & desenvolvimento
Estereoisomerismo
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 ((E)-3-(2-1H-benzo(d)imidazol-1-yl)-1-((4-chlorobenzyl)oxy)imino)ethyl)-2H-chromen-2-one); 0 (Anti-Bacterial Agents); 0 (Anti-Infective Agents); 0 (Benzimidazoles); 0 (Coumarins); 0 (Hydrocarbons, Chlorinated)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170622
[St] Status:MEDLINE
[do] DOI:10.1038/ja.2017.70


  3 / 2679 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28511289
[Au] Autor:Cuthbert RJ; Fragkakis EM; Dunsmuir R; Li Z; Coles M; Marzo-Ortega H; Giannoudis PV; Jones E; El-Sherbiny YM; McGonagle D
[Ad] Endereço:University of Leeds, Leeds, UK.
[Ti] Título:Brief Report: Group 3 Innate Lymphoid Cells in Human Enthesis.
[So] Source:Arthritis Rheumatol;69(9):1816-1822, 2017 Sep.
[Is] ISSN:2326-5205
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Group 3 innate lymphoid cells (ILC3s) play a pivotal role in barrier tissues such as the gut and the skin, two important sites of disease in spondyloarthritis (SpA). This study was undertaken to investigate whether normal or injured human enthesis, a key target tissue in early SpA, harbors ILC3s in entheseal soft tissue and adjacent perientheseal bone. METHODS: Interspinous ligament and spinous process bone from donors with no systemic inflammatory disease were collected, enzymatically digested, and immunophenotyped. The immunologic profile of entheseal cells was examined, and the transcriptional profile of sorted ILC3s was compared to that of ILC3s isolated from SpA synovial fluid (SF). To assess the ability of entheseal tissue to produce interleukin-17 (IL-17) and IL-22, entheseal digests were stimulated with IL-23 and IL-1ß. Osteoarthritic and ruptured Achilles tendon tissue was examined histologically. RESULTS: The proportion of ILCs in human entheseal soft tissue was higher than that in peripheral blood (P = 0.008); entheseal soft tissue and perientheseal bone both had a higher proportion of NKp44+ ILC3s (P = 0.001 and P = 0.043, respectively). Studies of retinoic acid receptor-related orphan nuclear receptor γt (RORγt), STAT3, and IL-23 receptor transcript expression validated the entheseal ILC3 phenotype. Cytokine transcript expression was similar in ILC3s isolated from enthesis and from SpA SF. Stimulation of normal entheseal digests with IL-23/IL-1ß led to up-regulation of IL-17A transcript, and histologic examination of injured/damaged entheses revealed the presence of RORγt-expressing cells. CONCLUSION: This work shows that human enthesis harbors a resident population of ILC3s, with the potential to participate in the pathogenesis of SpA.
[Mh] Termos MeSH primário: Células do Tecido Conjuntivo/imunologia
Imunidade Inata/imunologia
Linfócitos/imunologia
Espondilartrite/imunologia
[Mh] Termos MeSH secundário: Tendão do Calcâneo/imunologia
Citocinas/metabolismo
Seres Humanos
Interleucina-17/biossíntese
Interleucinas/biossíntese
Osteoartrite/imunologia
Líquido Sinovial/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 0 (Interleukin-17); 0 (Interleukins); 0 (interleukin-22)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170517
[St] Status:MEDLINE
[do] DOI:10.1002/art.40150


  4 / 2679 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28208829
[Au] Autor:Radu BM; Banciu A; Banciu DD; Radu M; Cretoiu D; Cretoiu SM
[Ad] Endereço:Department of Neuroscience, Biomedicine and Movement Sciences, University of Verona, Strada Le Grazie 8, Verona 37134, Italy. beatrice.mihaela.radu@gmail.com.
[Ti] Título:Calcium Signaling in Interstitial Cells: Focus on Telocytes.
[So] Source:Int J Mol Sci;18(2), 2017 Feb 13.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:In this review, we describe the current knowledge on calcium signaling pathways in interstitial cells with a special focus on interstitial cells of Cajal (ICCs), interstitial Cajal-like cells (ICLCs), and telocytes. In detail, we present the generation of Ca oscillations, the inositol triphosphate (IP3)/Ca signaling pathway and modulation exerted by cytokines and vasoactive agents on calcium signaling in interstitial cells. We discuss the physiology and alterations of calcium signaling in interstitial cells, and in particular in telocytes. We describe the physiological contribution of calcium signaling in interstitial cells to the pacemaking activity (e.g., intestinal, urinary, uterine or vascular pacemaking activity) and to the reproductive function. We also present the pathological contribution of calcium signaling in interstitial cells to the aortic valve calcification or intestinal inflammation. Moreover, we summarize the current knowledge of the role played by calcium signaling in telocytes in the uterine, cardiac and urinary physiology, and also in various pathologies, including immune response, uterine and cardiac pathologies.
[Mh] Termos MeSH primário: Sinalização do Cálcio
Cálcio/metabolismo
Células do Tecido Conjuntivo/metabolismo
Telócitos/metabolismo
[Mh] Termos MeSH secundário: Animais
Sinalização do Cálcio/efeitos dos fármacos
Células do Tecido Conjuntivo/classificação
Células do Tecido Conjuntivo/ultraestrutura
Citocinas/metabolismo
Seres Humanos
Imunofenotipagem
Inflamação/metabolismo
Inflamação/patologia
Células Intersticiais de Cajal/metabolismo
Células Intersticiais de Cajal/ultraestrutura
Fenótipo
Telócitos/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Cytokines); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170420
[Lr] Data última revisão:
170420
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170218
[St] Status:MEDLINE


  5 / 2679 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28114113
[Au] Autor:Oliver PA; Thomson RM
[Ad] Endereço:Department of Physics, Carleton Laboratory for Radiotherapy Physics, Carleton University, Ottawa, K1S 5B6, Canada.
[Ti] Título:A Monte Carlo study of macroscopic and microscopic dose descriptors for kilovoltage cellular dosimetry.
[So] Source:Phys Med Biol;62(4):1417-1436, 2017 Feb 21.
[Is] ISSN:1361-6560
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This work investigates how doses to cellular targets depend on cell morphology, as well as relations between cellular doses and doses to bulk tissues and water. Multicellular models of five healthy and cancerous soft tissues are developed based on typical values of cell compartment sizes, elemental compositions and number densities found in the literature. Cells are modelled as two concentric spheres with nucleus and cytoplasm compartments. Monte Carlo simulations are used to calculate the absorbed dose to the nucleus and cytoplasm for incident photon energies of 20-370 keV, relevant for brachytherapy, diagnostic radiology, and out-of-field radiation in higher-energy external beam radiotherapy. Simulations involving cell clusters, single cells and single nuclear cavities are carried out for cell radii between 5 and [Formula: see text]m, and nuclear radii between 2 and [Formula: see text]m. Seven nucleus and cytoplasm elemental compositions representative of animal cells are considered. The presence of a cytoplasm, extracellular matrix and surrounding cells can affect the nuclear dose by up to [Formula: see text]. Differences in cell and nucleus size can affect dose to the nucleus (cytoplasm) of the central cell in a cluster of 13 cells by up to [Formula: see text] ([Formula: see text]). Furthermore, the results of this study demonstrate that neither water nor bulk tissue are reliable substitutes for subcellular targets for incident photon energies <50 keV: nuclear (cytoplasm) doses differ from dose-to-medium by up to [Formula: see text] ([Formula: see text]), and from dose-to-water by up to [Formula: see text] ([Formula: see text]). The largest differences between dose descriptors are seen for the lowest incident photon energies; differences are less than [Formula: see text] for energies [Formula: see text]90 keV. The sensitivity of results with regard to the parameters of the microscopic tissue structure model and cell model geometry, and the importance of the nucleus and cytoplasm as targets for radiation-induced cell death emphasize the importance of accurate models for cellular dosimetry studies.
[Mh] Termos MeSH primário: Braquiterapia/métodos
Núcleo Celular/efeitos da radiação
Citoplasma/efeitos da radiação
Dose de Radiação
Planejamento da Radioterapia Assistida por Computador/métodos
[Mh] Termos MeSH secundário: Células do Tecido Conjuntivo/efeitos da radiação
Seres Humanos
Modelos Teóricos
Método de Monte Carlo
Células Musculares/efeitos da radiação
Fótons
Dosímetros de Radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170124
[St] Status:MEDLINE
[do] DOI:10.1088/1361-6560/aa5136


  6 / 2679 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27338002
[Au] Autor:Songia P; Branchetti E; Parolari A; Myasoedova V; Ferrari G; Alamanni F; Tremoli E; Poggio P
[Ad] Endereço:Centro Cardiologico Monzino IRCCS, Milan, Italy; Università degli Studi di Milano, Dipartimento di Scienze Farmacologiche e Biomolecolari, Milan, Italy.
[Ti] Título:Mitral valve endothelial cells secrete osteoprotegerin during endothelial mesenchymal transition.
[So] Source:J Mol Cell Cardiol;98:48-57, 2016 Sep.
[Is] ISSN:1095-8584
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIMS: Mitral valve prolapse (MVP) has a prevalence of 3% in the general population, affecting >176 million people worldwide. Despite this, little is known about the molecular and cellular mechanisms involved in the progression of MVP and surgical intervention is the only available option. In this study we investigated the role of osteoprotegerin (OPG) during endothelial to mesenchymal transition (EndMT) in MVP. METHODS AND RESULTS: VECs and VICs were isolated from posterior mitral valve leaflets of patients undergoing mitral valve repair (n=25). Plasma was collected from 57 subjects (29 controls and 28 MVP patients). Overexpression of OPG during EndMT followed by autocrine effects characterised by reactive oxygen species increment and accelerated migration was documented. In addition, OPG increased VIC proliferation. Finally, OPG plasma levels were significantly higher in MVP patients compared to control subjects and the area under the ROC curve was 0.92. CONCLUSION: EndMT has been recognised as a possible pathological mechanism for MVP. For the first time, we report the involvement of OPG in cellular and molecular changes in MVP isolated cells. In addition, we detected elevated circulating OPG levels in MVP patients when compared to controls, which supports the hypothesis that OPG is involved in MVP development and progression.
[Mh] Termos MeSH primário: Células Endoteliais/metabolismo
Células Endoteliais/patologia
Transição Epitelial-Mesenquimal
Valva Mitral/metabolismo
Valva Mitral/patologia
Osteoprotegerina/secreção
[Mh] Termos MeSH secundário: Adulto
Estudos de Casos e Controles
Movimento Celular
Células Cultivadas
Colágeno/metabolismo
Células do Tecido Conjuntivo/metabolismo
Transição Epitelial-Mesenquimal/genética
Feminino
Expressão Gênica
Seres Humanos
Masculino
Metaloproteinases da Matriz/metabolismo
Prolapso da Valva Mitral/diagnóstico
Prolapso da Valva Mitral/genética
Prolapso da Valva Mitral/metabolismo
Prolapso da Valva Mitral/cirurgia
Osteoprotegerina/genética
Fenótipo
Espécies Reativas de Oxigênio/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Osteoprotegerin); 0 (Reactive Oxygen Species); 9007-34-5 (Collagen); EC 3.4.24.- (Matrix Metalloproteinases)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170902
[Lr] Data última revisão:
170902
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160625
[St] Status:MEDLINE


  7 / 2679 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27316294
[Au] Autor:McCusker CD; Diaz-Castillo C; Sosnik J; Q Phan A; Gardiner DM
[Ad] Endereço:Department of Biology, University of Massachusetts Boston, MA 02125, USA. Electronic address: Catherine.mccusker@umb.edu.
[Ti] Título:Cartilage and bone cells do not participate in skeletal regeneration in Ambystoma mexicanum limbs.
[So] Source:Dev Biol;416(1):26-33, 2016 08 01.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Mexican Axolotl is one of the few tetrapod species that is capable of regenerating complete skeletal elements in injured adult limbs. Whether the skeleton (bone and cartilage) plays a role in the patterning and contribution to the skeletal regenerate is currently unresolved. We tested the induction of pattern formation, the effect on cell proliferation, and contributions of skeletal tissues (cartilage, bone, and periosteum) to the regenerating axolotl limb. We found that bone tissue grafts from transgenic donors expressing GFP fail to induce pattern formation and do not contribute to the newly regenerated skeleton. Periosteum tissue grafts, on the other hand, have both of these activities. These observations reveal that skeletal tissue does not contribute to the regeneration of skeletal elements; rather, these structures are patterned by and derived from cells of non-skeletal connective tissue origin.
[Mh] Termos MeSH primário: Osso e Ossos/fisiologia
Cartilagem/fisiologia
Regeneração/fisiologia
[Mh] Termos MeSH secundário: Ambystoma mexicanum
Animais
Células do Tecido Conjuntivo/fisiologia
Extremidades
Periósteo/citologia
Periósteo/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160619
[St] Status:MEDLINE


  8 / 2679 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27135604
[Au] Autor:Dwyer DF; Barrett NA; Austen KF; Immunological Genome Project Consortium
[Ad] Endereço:Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital, Boston, Massachusetts, USA.
[Ti] Título:Expression profiling of constitutive mast cells reveals a unique identity within the immune system.
[So] Source:Nat Immunol;17(7):878-87, 2016 Jul.
[Is] ISSN:1529-2916
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mast cells are evolutionarily ancient sentinel cells. Like basophils, mast cells express the high-affinity receptor for immunoglobulin E (IgE) and have been linked to host defense and diverse immune-system-mediated diseases. To better characterize the function of these cells, we assessed the transcriptional profiles of mast cells isolated from peripheral connective tissues and basophils isolated from spleen and blood. We found that mast cells were transcriptionally distinct, clustering independently from all other profiled cells, and that mast cells demonstrated considerably greater heterogeneity across tissues than previously appreciated. We observed minimal homology between mast cells and basophils, which shared more overlap with other circulating granulocytes than with mast cells. The derivation of mast-cell and basophil transcriptional signatures underscores their differential capacities to detect environmental signals and influence the inflammatory milieu.
[Mh] Termos MeSH primário: Basófilos/fisiologia
Células Sanguíneas/fisiologia
Células do Tecido Conjuntivo/fisiologia
Mastócitos/fisiologia
Baço/citologia
[Mh] Termos MeSH secundário: Animais
Separação Celular
Células Cultivadas
Citometria de Fluxo
Perfilação da Expressão Gênica
Imunoglobulina E/metabolismo
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Análise Serial de Tecidos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
37341-29-0 (Immunoglobulin E)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170531
[Lr] Data última revisão:
170531
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160503
[St] Status:MEDLINE
[do] DOI:10.1038/ni.3445


  9 / 2679 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26948996
[Au] Autor:Galligan CL; Keystone EC; Fish EN
[Ad] Endereço:Toronto General Research Institute, University Health Network, Toronto, Canada; Department of Immunology, University of Toronto, Canada.
[Ti] Título:Fibrocyte and T cell interactions promote disease pathogenesis in rheumatoid arthritis.
[So] Source:J Autoimmun;69:38-50, 2016 May.
[Is] ISSN:1095-9157
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Rheumatoid arthritis (RA) is a systemic autoimmune disease. We previously identified a circulating cell population, fibrocytes, which is activated early in disease. As RA is characterized by the formation of autoantibodies and autoreactive T cells, which often precede symptom onset, the objective of these studies was to characterize fibrocyte activation in the context of T cell activation. Multidimensional flow cytometry was used to characterize the activation status of peripheral blood (PB) fibrocytes and T cells derived from RA patients with different levels of disease activity. Compared to healthy controls, fibrocytes from RA patients exhibited increased activation, denoted as elevated levels of phosphorylation of STAT3 and NF-κB. RA patients had higher numbers of circulating activated Th17 cells and Tregs compared with healthy controls, Th17 cell numbers being higher in patients with moderate to high disease activity. Additionally, increased numbers of FOXP3+ RORγt+ double positive CD4+ T cells were observed in RA patients with more severe disease. Our data confirm that circulating fibrocytes are expanded in RA and that there is a direct correlation between the increase in number of activated fibrocytes and increased number of CD4+ T cells. Moreover, our data suggest that interactions between circulating fibrocytes and activated T cells may promote disease activity. Specifically, we provide in vitro evidence that mouse-derived CD4+ T cells produce GM-CSF which induces fibrocyte proliferation. In turn, activated fibrocytes produce IL-6, promoting Th17 polarization.
[Mh] Termos MeSH primário: Artrite Reumatoide/etiologia
Artrite Reumatoide/metabolismo
Comunicação Celular
Células do Tecido Conjuntivo/metabolismo
Subpopulações de Linfócitos T/imunologia
Subpopulações de Linfócitos T/metabolismo
[Mh] Termos MeSH secundário: Adulto
Animais
Artrite Reumatoide/diagnóstico
Biomarcadores
Estudos de Casos e Controles
Citocinas/metabolismo
Feminino
Seres Humanos
Imunofenotipagem
Ativação Linfocitária
Contagem de Linfócitos
Masculino
Camundongos
Meia-Idade
Fenótipo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biomarkers); 0 (Cytokines)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160308
[St] Status:MEDLINE


  10 / 2679 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26921601
[Au] Autor:Chagin AS
[Ad] Endereço:Department of Physiology and Pharmacology, Karolinska Institutet, Sweden. Electronic address: Andrei.chagin@ki.se.
[Ti] Título:Effectors of mTOR-autophagy pathway: targeting cancer, affecting the skeleton.
[So] Source:Curr Opin Pharmacol;28:1-7, 2016 Jun.
[Is] ISSN:1471-4973
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Although some modulators of autophagy are emerging as drugs or supplements for anti-cancer therapy, the effects of these compounds on normal tissues must be examined carefully. Here, I review the role of autophagy in skeletal tissues in this context. First, I briefly review preclinical studies indicating the role of autophagy in cancer, as well as related on-going clinical trials. Thereafter, the role of autophagy in the physiology of skeletal tissues is discussed, with a focus on recent genetic preclinical studies. Specifically, I discuss the mTOR-autophagy pathway in relationship to epiphyseal chondrocytes, articular chondrocytes, osteoblasts, osteocytes and osteoclasts and potential side effects of targeting either mTOR pathway or autophagy in general in connection with anti-cancer therapy. Current preclinical findings indicate that inhibiting autophagy will not seriously reduce bone mass and enhance osteoporosis. However, inhibition of autophagy might damage articular cartilage and cause osteoarthritis, whereas treatment with rapalogs might result in relatively beneficial effects on articular cartilage. Modulation of the mTOR pathway or autophagy during childhood may have an undesirable influence on adult height, as well as acquisition of bone mass.
[Mh] Termos MeSH primário: Autofagia/efeitos dos fármacos
Neoplasias/tratamento farmacológico
Serina-Treonina Quinases TOR/metabolismo
[Mh] Termos MeSH secundário: Adulto
Animais
Antineoplásicos/efeitos adversos
Antineoplásicos/farmacologia
Estatura
Cartilagem Articular/efeitos dos fármacos
Criança
Células do Tecido Conjuntivo/citologia
Seres Humanos
Neoplasias/patologia
Osteoartrite/etiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Antineoplastic Agents); EC 2.7.1.1 (MTOR protein, human); EC 2.7.1.1 (TOR Serine-Threonine Kinases)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170724
[Lr] Data última revisão:
170724
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160228
[St] Status:MEDLINE



página 1 de 268 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde