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  1 / 18037 MEDLINE  
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[PMID]:29367486
[Au] Autor:Miyazawa N; Yoshimoto H; Kurihara S; Hamaya T; Eguchi F
[Ad] Endereço:Faculty of Regional Environment Science, Tokyo University of Agriculture.
[Ti] Título:Improvement of Diet-induced Obesity by Ingestion of Mushroom Chitosan Prepared from Flammulina velutipes.
[So] Source:J Oleo Sci;67(2):245-254, 2018 Feb 01.
[Is] ISSN:1347-3352
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:The anti-obesity effects of mushroom chitosan prepared from Flammulina velutipes were investigated using an animal model with diet-induced obesity. In this study, 5-week-old imprinting control region (ICR) mice were divided into six groups of 10 mice each and fed different diets based on the MF powdered diet (standard diet) for 6 weeks: standard diet control group, high-fat diet control group (induced dietary obesity) consisting of the standard diet and 20% lard, and mushroom chitosan groups consisting of the high-fat diet with mushroom chitosan added at 100, 500, 1,000, and 2,000 mg/kg body weight. On the final day of the experiment, mean body weight was 39.1 g in the high-fat control group and 36.3 g in the 2,000 mg/kg mushroom chitosan group, compared to 35.8 g in the standard diet control group. In the mushroom chitosan groups, a dose-dependent suppression of weight gain and marked improvements in serum triglycerides, total cholesterol, LDL-cholesterol, and HDL-cholesterol were found. The mushroom chitosan groups showed fewer and smaller fat deposits in liver cells than the high-fat diet control group, and liver weight was significantly reduced. Glutamic oxaloacetic transaminase (GOT) and glutamate pyruvic transaminase (GPT), which are indices of the hepatic function, all showed dose-dependent improvement with mushroom chitosan administration. These results suggested that mushroom chitosan acts to suppress enlargement of the liver from fat deposition resulting from a high-fat diet and to restore hepatic function. The lipid content of feces showed a marked increase correlated with the mushroom chitosan dose. These findings suggest the potential use of mushroom chitosan as a functional food ingredient that contributes to the prevention or improvement of dietary obesity by inhibiting digestion and absorption of fats in the digestive tract and simultaneously promotes lipolysis in adipocytes.
[Mh] Termos MeSH primário: Quitosana/administração & dosagem
Quitosana/isolamento & purificação
Dieta Hiperlipídica/efeitos adversos
Flammulina/química
Obesidade/prevenção & controle
Fitoterapia
[Mh] Termos MeSH secundário: Adipócitos/metabolismo
Administração Oral
Animais
Fármacos Antiobesidade
Quitosana/farmacologia
Modelos Animais de Doenças
Relação Dose-Resposta a Droga
Lipólise/efeitos dos fármacos
Masculino
Camundongos Endogâmicos ICR
Hepatopatia Gordurosa não Alcoólica/prevenção & controle
Obesidade/etiologia
Obesidade/metabolismo
Ganho de Peso/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Obesity Agents); 9012-76-4 (Chitosan)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE
[do] DOI:10.5650/jos.ess17159


  2 / 18037 MEDLINE  
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[PMID]:29214781
[Au] Autor:Kim B; Choi KM; Yim HS; Park HT; Yim JH; Lee MG
[Ad] Endereço:Department of Physiology, Korea University College of Medicine, Seoul, Korea.
[Ti] Título:Adipogenic and Lipolytic Effects of Ascorbic Acid in Ovariectomized Rats.
[So] Source:Yonsei Med J;59(1):85-91, 2018 Jan.
[Is] ISSN:1976-2437
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Ascorbic acid has been reported to have an adipogenic effect on 3T3-L1 preadipocytes, while evidence also suggests that ascorbic acid reduces body weight in humans. In this study, we tested the effects of ascorbic acid on adipogenesis and the balance of lipid accumulation in ovariectomized rats, in addition to long-term culture of differentiated 3T3-L1 adipocytes. MATERIALS AND METHODS: Murine 3T3-L1 fibroblasts and ovariectomized rats were treated with ascorbic acid at various time points. In vitro adipogenesis was analyzed by Oil Red O staining, and in vivo body fat was measured by a body composition analyzer using nuclear magnetic resonance. RESULTS: When ascorbic acid was applied during an early time point in 3T3-L1 preadipocyte differentiation and after bilateral ovariectomy (OVX) in rats, adipogenesis and fat mass gain significantly increased, respectively. However, lipid accumulation in well-differentiated 3T3-L1 adipocytes showed a significant reduction when ascorbic acid was applied after differentiation (10 days after induction). Also, oral ascorbic acid administration 4 weeks after OVX in rats significantly reduced both body weight and subcutaneous fat layer. In comparison to the results of ascorbic acid, which is a well-known cofactor for an enzyme of collagen synthesis, and the antioxidant ramalin, a potent antioxidant but not a cofactor, showed only a lipolytic effect in well-differentiated 3T3-L1 adipocytes, not an adipogenic effect. CONCLUSION: Taking these results into account, we concluded that ascorbic acid has both an adipogenic effect as a cofactor of an enzymatic process and a lipolytic effect as an antioxidant.
[Mh] Termos MeSH primário: Adipogenia/efeitos dos fármacos
Ácido Ascórbico/farmacologia
Lipólise/efeitos dos fármacos
Ovariectomia
[Mh] Termos MeSH secundário: Células 3T3-L1
Adipócitos/efeitos dos fármacos
Adipócitos/metabolismo
Animais
Antioxidantes/farmacologia
Composição Corporal/efeitos dos fármacos
Peso Corporal/efeitos dos fármacos
Diferenciação Celular/efeitos dos fármacos
Feminino
Fibroblastos/efeitos dos fármacos
Fibroblastos/metabolismo
Camundongos
Ratos Sprague-Dawley
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); PQ6CK8PD0R (Ascorbic Acid)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171208
[St] Status:MEDLINE
[do] DOI:10.3349/ymj.2018.59.1.85


  3 / 18037 MEDLINE  
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[PMID]:28637122
[Au] Autor:Wang ZG; Mi J; Wang XR; Huo YY; Peng YJ; Zhang HM; Gao Y; Zhang HL
[Ad] Endereço:a Department of Pharmaceutical Analysis , Heilongjiang University of Chinese Medicine , Harbin , China.
[Ti] Título:A new cinnamic acid glycoside from roots of Heracleum dissectum.
[So] Source:Nat Prod Res;32(2):133-140, 2018 Jan.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:From the roots of Heracleum dissectum Lebb., one new cinnamic acid glycoside derivative named dissectumoside (1), together with eight known compounds including three phenolics, three phenolic glycosides and two phenylpropanoic glycoside were isolated using various chromatographic methods. Among them compound 2-9 was isolated from the plant for the first time. Their structures were elucidated and identified on the basis of their physicochemical properties and by extensive analyses of NMR spectroscopy and high-resolution mass spectrometry. The results of triglyceride accumulation screening in 3T3-L1 cells showed that compounds 1, 5 and 9 exhibited significantly accelerating activities of adipogenesis in adipocytes.
[Mh] Termos MeSH primário: Cinamatos/isolamento & purificação
Glicosídeos/isolamento & purificação
Heracleum/química
Extratos Vegetais/química
[Mh] Termos MeSH secundário: Células 3T3-L1
Adipócitos/efeitos dos fármacos
Adipogenia/efeitos dos fármacos
Animais
Glicosídeos Cardíacos
Cinamatos/química
Glicosídeos/química
Camundongos
Fenóis/química
Raízes de Plantas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cardiac Glycosides); 0 (Cinnamates); 0 (Glycosides); 0 (Phenols); 0 (Plant Extracts); U14A832J8D (cinnamic acid)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170623
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2017.1340285


  4 / 18037 MEDLINE  
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[PMID]:28471118
[Au] Autor:Dong J; Zhu G; Wang TC; Shi FS
[Ad] Endereço:Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
[Ti] Título:Ginsenoside Rg1 promotes neural differentiation of mouse adipose-derived stem cells via the miRNA-124 signaling pathway.
[So] Source:J Zhejiang Univ Sci B;18(5):445-448, 2017 May.
[Is] ISSN:1862-1783
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:We have explored the role of ginsenoside Rg1 in promoting the differentiation of mouse adipose-derived stem cells (mADSC) towards the neuronal lineage. The central nervous system has long been regarded as incapable of self-repair; therefore neuronal differentiation from stem cells is of great interest. However, the use of embryonic stem cells is limited due to their inaccessibility and for ethical reasons, so the search is on for alternative pluripotent cells capable of differentiating into neuronal cells. Adipose-derived stem cells (ADSC) can differentiate into different cell types, including neuronal cells: their accessibility, low risk, and capacity for long-term growth and self-renewal have made them the preferred stem cell type for clinical applications. Several methods have been indicated for promoting the neuronal differentiation of ADSC, but the mechanism of this process has not been clearly identified. As our previous study showed that microRNA-124 (miRNA-124) plays a positive role in promoting the neural differentiation of ADSC, we wanted to find reagents that can upregulate miRNA-124 expression during neural differentiation.
[Mh] Termos MeSH primário: Ginsenosídeos/administração & dosagem
MicroRNAs/metabolismo
Células-Tronco Neurais/efeitos dos fármacos
Células-Tronco Neurais/fisiologia
Neurogênese/fisiologia
Neurônios/fisiologia
[Mh] Termos MeSH secundário: Adipócitos/citologia
Adipócitos/fisiologia
Animais
Diferenciação Celular/efeitos dos fármacos
Diferenciação Celular/fisiologia
Células Cultivadas
Relação Dose-Resposta a Droga
Camundongos
Células-Tronco Neurais/citologia
Neurogênese/efeitos dos fármacos
Neurônios/citologia
Neurônios/efeitos dos fármacos
Transdução de Sinais/efeitos dos fármacos
Transdução de Sinais/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ginsenosides); 0 (MicroRNAs); 0 (Mirn124 microRNA, mouse); PJ788634QY (ginsenoside Rg1)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1631/jzus.B1600355


  5 / 18037 MEDLINE  
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[PMID]:28464921
[Au] Autor:Montali M; Panvini FM; Barachini S; Ronca F; Carnicelli V; Mazzoni S; Petrini I; Pacini S
[Ad] Endereço:Department of Clinical and Experimental Medicine, Hematology Division, University of Pisa, Via Roma 56, 56126, Pisa, Italy.
[Ti] Título:Human adult mesangiogenic progenitor cells reveal an early angiogenic potential, which is lost after mesengenic differentiation.
[So] Source:Stem Cell Res Ther;8(1):106, 2017 May 02.
[Is] ISSN:1757-6512
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Mesangiogenic progenitor cells (MPCs) have shown the ability to differentiate in-vitro toward mesenchymal stromal cells (MSCs) as well as angiogenic potential. MPCs have so far been described in detail as progenitors of the mesodermal lineage and appear to be of great significance in tissue regeneration and in hemopoietic niche regulation. On the contrary, information regarding the MPC angiogenic process is still incomplete and requires further clarification. In particular, genuine MPC angiogenic potential should be confirmed in-vivo. METHODS: In the present article, markers and functions associated with angiogenic cells have been dissected. MPCs freshly isolated from human bone marrow have been induced to differentiate into exponentially growing MSCs (P2-MSCs). Cells have been characterized and angiogenesis-related gene expression was evaluated before and after mesengenic differentiation. Moreover, angiogenic potential has been tested by in-vitro and in-vivo functional assays. RESULTS: MPCs showed a distinctive gene expression profile, acetylated-low density lipoprotein uptake, and transendothelial migration capacity. However, mature endothelial markers and functions of endothelial cells, including the ability to form new capillaries, were absent, thus suggesting MPCs to be very immature endothelial progenitors. MPCs showed marked 3D spheroid sprouting activating the related molecular machinery, a clear in-vitro indication of early angiogenesis. Indeed, MPCs applied to chicken chorioallantoic membrane induced and participated in neovessel formation. All of these features were lost in mesengenic terminally differentiated P2-MSCs, showing definite separation of the two differentiation lineages. CONCLUSION: Our results confirm the bona-fide angiogenic potential of MPCs and suggest that the high variability reported for MSC cultures, responsible for the controversies regarding MSC angiogenic potential, could be correlated to variable percentages of co-isolated MPCs in the different culture conditions so far used.
[Mh] Termos MeSH primário: Células-Tronco Adultas/citologia
Diferenciação Celular
Células Mesenquimais Estromais/citologia
Neovascularização Fisiológica
[Mh] Termos MeSH secundário: Adipócitos/citologia
Adipócitos/metabolismo
Células-Tronco Adultas/metabolismo
Células Cultivadas
Feminino
Células Endoteliais da Veia Umbilical Humana/citologia
Células Endoteliais da Veia Umbilical Humana/metabolismo
Seres Humanos
Masculino
Células Mesenquimais Estromais/metabolismo
Meia-Idade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1186/s13287-017-0562-x


  6 / 18037 MEDLINE  
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[PMID]:29308837
[Au] Autor:Prokudina ES; Maslov LN; Ivanov VV; Bespalova ID; Pismennyi DS; Voronkov NS
[Ti] Título:The Role of Reactive Oxygen Species in the Pathogenesis of Adipocyte Dysfunction in Metabolic Syndrome. Prospects of Pharmacological Correction.
[So] Source:Vestn Ross Akad Med Nauk;72(1):11-6, 2017.
[Is] ISSN:0869-6047
[Cp] País de publicação:Russia (Federation)
[La] Idioma:eng
[Ab] Resumo:It is established that oxidative stress induces insulin resistance of adipocytes, increases secretion leptin, IL-6, TNF-α by adipocytes. Adiponectin secretion by adipocytes is reduced after the action of reactive oxygen species. Metabolic syndrome contributes to oxidative stress in adipose tissue, on the one hand due to the activation of production of reactive oxygen species by adipocyte NADPH-oxidase, and on the other hand by reducing the antioxidant defense adipocytes. It is found that obesity itself can induce oxidative stress. Chronic stress, glucocorticoids, mineralocorticoids, angiotensin-II, TNF-α play an important role in the pathogenesis of oxidative stress of adipocytes. Metformin remains the cure for the treatment of insulin resistance. The positive results in the treatment of metabolic syndrome by losartan were obtained. Antioxidants and flavonoids exhibit a positive impact on the course of the experimental metabolic syndrome.
[Mh] Termos MeSH primário: Adipócitos/fisiologia
Resistência à Insulina
Síndrome Metabólica
Metformina/farmacologia
Estresse Oxidativo
[Mh] Termos MeSH secundário: Seres Humanos
Hipoglicemiantes/farmacologia
Síndrome Metabólica/tratamento farmacológico
Síndrome Metabólica/metabolismo
Síndrome Metabólica/patologia
Estresse Oxidativo/efeitos dos fármacos
Estresse Oxidativo/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Hypoglycemic Agents); 9100L32L2N (Metformin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180109
[St] Status:MEDLINE
[do] DOI:10.15690/vramn798


  7 / 18037 MEDLINE  
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[PMID]:28454542
[Au] Autor:Itabe H; Yamaguchi T; Nimura S; Sasabe N
[Ad] Endereço:Division of Biological Chemistry, Department of Molecular Biology, Showa University School of Pharmacy, 1-5-8 Hatanodai, Shinagawa, Tokyo, 142-8555, Japan. h-itabe@pharm.showa-u.ac.jp.
[Ti] Título:Perilipins: a diversity of intracellular lipid droplet proteins.
[So] Source:Lipids Health Dis;16(1):83, 2017 Apr 28.
[Is] ISSN:1476-511X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Intracellular lipid droplets (LDs) are found in a wide variety of cell types and have been recognized as organelles with unique spherical structures. Although LDs are not stable lipid-depots, they are active sites of neutral lipid metabolism, and comprise neutral lipid or cholesterol cores surrounded by phospholipid monolayers containing specialized proteins. However, sizes and protein compositions vary between cell and tissue types. Proteins of the perilipin family have been associated with surfaces of LDs and all carry a conserved 11-mer repeat motif. Accumulating evidence indicates that all perilipins are involved in LD formation and that all play roles in LD function under differing conditions. In this brief review, we summarize current knowledge of the roles of perilipins and lipid metabolizing enzymes in a variety of mammalian cell types.
[Mh] Termos MeSH primário: Adipócitos/metabolismo
Hidroxiesteroide Desidrogenases/genética
Gotículas Lipídicas/metabolismo
Metabolismo dos Lipídeos/genética
Perilipinas/genética
[Mh] Termos MeSH secundário: Animais
Colesterol/metabolismo
Retículo Endoplasmático/metabolismo
Regulação da Expressão Gênica
Seres Humanos
Hidroxiesteroide Desidrogenases/metabolismo
Mitocôndrias/metabolismo
Perilipinas/química
Perilipinas/classificação
Perilipinas/metabolismo
Domínios Proteicos
Transdução de Sinais
Triglicerídeos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Perilipins); 0 (Triglycerides); 97C5T2UQ7J (Cholesterol); EC 1.1.- (Hydroxysteroid Dehydrogenases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE
[do] DOI:10.1186/s12944-017-0473-y


  8 / 18037 MEDLINE  
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[PMID]:29186386
[Au] Autor:Bonnet L; Karkeni E; Couturier C; Astier J; Dalifard J; Defoort C; Svilar L; Martin JC; Tourniaire F; Landrier JF
[Ad] Endereço:NORT, Aix-Marseille Université, INRA, INSERM, Marseille, France.
[Ti] Título:Gene Expression Pattern in Response to Cholecalciferol Supplementation Highlights Cubilin as a Major Protein of 25(OH)D Uptake in Adipocytes and Male Mice White Adipose Tissue.
[So] Source:Endocrinology;159(2):957-966, 2018 02 01.
[Is] ISSN:1945-7170
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:It is well established that the active form of vitamin D (i.e., 1,25-dihydroxyvitamin D [1,25(OH)2D]) regulates the expression of genes involved in its own metabolism and transport in the kidney and possibly in the liver. However, little is known about the transcriptional impact of cholecalciferol supplementation on white adipose tissue (WAT) and adipocytes, which are a major site of vitamin D and 25-hydroxyvitamin D [25(OH)D] storage in the organism. To fill this gap, we investigated the impact of cholecalciferol supplementation in WAT via a panel of genes coding for enzymes and proteins involved in vitamin D metabolism and uptake. Mice supplemented with cholecalciferol (15,000 IU/kg of body weight per day) for 4 days showed decreased messenger RNA (mRNA) levels of proteins involved in cholecalciferol metabolism (Cyp24a1, Cyp27a1) and decreased cubilin mRNA levels in WAT. These data were partly confirmed in 3T3-L1 adipocytes incubated with 1,25(OH)2D. The downregulation of cubilin mRNA observed in WAT and in 3T3-L1 was confirmed at the protein level in WAT and at the mRNA level in human primary adipocytes. Vitamin D receptor (VDR) agonist (EB1089) and RNA interference approaches demonstrated that VDR was involved in this regulation. Furthermore, chemical inhibitor and RNA inference analysis demonstrated that cubilin was involved in 25(OH)D uptake by adipocytes. This study established an overall snapshot of the genes regulated by cholecalciferol in mouse WAT and cell-autonomously in adipocytes. We highlighted that the regulation of cubilin expression was mediated by a VDR-dependent mechanism, and we demonstrated that cubilin was involved in 25(OH)D uptake by adipocytes.
[Mh] Termos MeSH primário: Adipócitos/efeitos dos fármacos
Tecido Adiposo Branco/efeitos dos fármacos
Colecalciferol/farmacologia
Receptores de Superfície Celular/genética
Vitamina D/análogos & derivados
[Mh] Termos MeSH secundário: Células 3T3-L1
Adipócitos/metabolismo
Tecido Adiposo Branco/metabolismo
Animais
Células Cultivadas
Suplementos Nutricionais
Feminino
Perfilação da Expressão Gênica
Regulação da Expressão Gênica/efeitos dos fármacos
Seres Humanos
Metabolismo dos Lipídeos/efeitos dos fármacos
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Receptores de Calcitriol/genética
Receptores de Calcitriol/metabolismo
Receptores de Superfície Celular/metabolismo
Vitamina D/farmacocinética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Receptors, Calcitriol); 0 (Receptors, Cell Surface); 0 (intrinsic factor-cobalamin receptor); 1406-16-2 (Vitamin D); 1C6V77QF41 (Cholecalciferol); 64719-49-9 (25-hydroxyvitamin D)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE
[do] DOI:10.1210/en.2017-00650


  9 / 18037 MEDLINE  
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[PMID]:29217197
[Au] Autor:Nuermaimaiti N; Liu J; Liang X; Jiao Y; Zhang D; Liu L; Meng X; Guan Y
[Ad] Endereço:Department of Biochemistry and Molecular Biology, Preclinical Medicine College, Xinjiang Medical University, No. 393 Xinyi Road, Urumqi 830011, Xinjiang, China.
[Ti] Título:Effect of lncRNA HOXA11-AS1 on adipocyte differentiation in human adipose-derived stem cells.
[So] Source:Biochem Biophys Res Commun;495(2):1878-1884, 2018 01 08.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:AIMS: To determine the role of lncRNA HOXA11-AS1 on adipocyte differentiation. METHODS: Human adipose-derived stem cells (hADSCs) were isolated from adipose tissues of patients and cultured in vitro, followed by knockdown of HOXA11-AS1. Then, adipocyte differentiation and expression of adipogenic-related genes (CEBP-α, DGAT2, CIDEC, and perilipin) were measured by RT-qPCR and Western blot. RESULTS: We demonstrated that knockdown of HOXA11-AS1 inhibited adipocyte differentiation, leading to suppression of adipogenic-related gene (CEBP-α, DGAT2, CIDEC, and perilipin) transcription, as well as decreased lipid accumulation in hADSCs. In addition, lncRNA HOXA11-AS1 was highly expressed in obese patients and significantly increased during the process of adipocyte differentiation. CONCLUSION: The results provide new insight into the molecular mechanism by which lncRNA HOXA11-AS1 is involved in adipogenesis and may have implications for the treatment of obesity and associated disorders.
[Mh] Termos MeSH primário: Adipócitos/metabolismo
Adipogenia
Tecido Adiposo/crescimento & desenvolvimento
Proteínas de Homeodomínio/metabolismo
Obesidade/metabolismo
RNA Longo não Codificante/metabolismo
Células-Tronco/fisiologia
[Mh] Termos MeSH secundário: Adipócitos/patologia
Tecido Adiposo/patologia
Diferenciação Celular/fisiologia
Células Cultivadas
Seres Humanos
Células-Tronco/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (HOXA11 protein, human); 0 (Homeodomain Proteins); 0 (RNA, Long Noncoding)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171209
[St] Status:MEDLINE


  10 / 18037 MEDLINE  
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[PMID]:29229387
[Au] Autor:Pan Y; Jing J; Qiao L; Liu J; Zhao J; An L; Li B; Wang W; Liang C; Liu W
[Ad] Endereço:College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
[Ti] Título:miR-124-3p affects the formation of intramuscular fat through alterations in branched chain amino acid consumption in sheep.
[So] Source:Biochem Biophys Res Commun;495(2):1769-1774, 2018 01 08.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Intramuscular fat is used to determine meat quality in animals; however, factors affecting branched chain amino acid (BCAA) catabolism, which fuels adipogenesis and lipogenesis, remain unclear. To better understand the post-transcriptional influence on BCAA catabolism during adipogenesis, we investigated the role of miR-124-3p. Stromal vascular fraction (SVF) cells were isolated from skeletal muscle of sheep, and induced to differentiate. We determined the roles of miR-124-3p and its predicted target, branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA), in adipogenic differentiation and lipogenesis of SVFs after overexpressing or inhibiting miR-124-3p or BCKDHA, respectively. miR-124-3p altered the luciferase activity of constructs containing 3'-UTR of BCKDHA and the formation of lipid droplets, along with the adipogenic markers and BCAA consumption. Besides, the adipogenic performance and BCAA consumption in BCKDHA-overexpressing or knocked-down SVFs and the expression of adipogenic marker genes were altered. We demonstrate that miR-124-3p is an important factor for adipogenesis and provide insights into the formation of intramuscular fat in animals.
[Mh] Termos MeSH primário: Adipogenia/genética
Aminoácidos de Cadeia Ramificada/metabolismo
MicroRNAs/genética
Ovinos/crescimento & desenvolvimento
Ovinos/genética
[Mh] Termos MeSH secundário: Regiões 3' não Traduzidas
3-Metil-2-Oxobutanoato Desidrogenase (Lipoamida)/genética
3-Metil-2-Oxobutanoato Desidrogenase (Lipoamida)/metabolismo
Adipócitos/citologia
Adipócitos/metabolismo
Adipogenia/fisiologia
Animais
Diferenciação Celular/genética
MicroRNAs/metabolismo
Músculo Esquelético/citologia
Músculo Esquelético/crescimento & desenvolvimento
Músculo Esquelético/metabolismo
Ovinos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (3' Untranslated Regions); 0 (Amino Acids, Branched-Chain); 0 (MicroRNAs); EC 1.2.4.4 (3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide))
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE



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