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[PMID]:29337061
[Au] Autor:Li Q; Qin Z; Nie F; Bi H; Zhao R; Pan B; Ma J; Xie X
[Ad] Endereço:Department of Plastic and Reconstructive Surgery, Peking University Third Hospital, Beijing, 100191, China.
[Ti] Título:Metabolic reprogramming in keloid fibroblasts: Aerobic glycolysis and a novel therapeutic strategy.
[So] Source:Biochem Biophys Res Commun;496(2):641-647, 2018 02 05.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Keloids, tumor-like fibroproliferative cutaneous lesions, were reported in metabolic disturbance. However, the metabolic character remains unclear. The purpose of this study is to determine if glycolytic reprogramming is important for the pathogenesis of keloids and to assess the inhibition potential of glycolysis in keloid treatment. An intracellular metabolic profile assay was used to compare metabolic phenotypes between normal skin fibroblasts and keloid fibroblasts (NFs and KFs). Our data indicated that KFs underwent reprogramming of their metabolic phonotype from oxidative phosphorylation to aerobic glycolysis (Warburg effect) with augmented glycolysis and glycolytic capacity. Both gene and protein assays showed that the expression of glycolytic enzymes was upregulated in KFs compared to NFs. Our data showed higher glucose influx and lactate production in KFs compared to NFs. Furthermore, the proliferation of KFs was suppressed in a dose-dependent and time-dependent manner after inhibition of glycolysis with 2-deoxy-glucose (2-DG). Taken together, these findings suggested that keloids underwent a reprogrammed metabolic phenotype of aerobic glycolysis. This was essential for keloid hyperplasia, and glycolytic inhibitors might provide a potential treatment for keloids.
[Mh] Termos MeSH primário: Fibroblastos/patologia
Queloide/patologia
[Mh] Termos MeSH secundário: Proliferação Celular/efeitos dos fármacos
Células Cultivadas
Desoxiglucose/farmacologia
Fibroblastos/efeitos dos fármacos
Fibroblastos/metabolismo
Regulação da Expressão Gênica
Glucose/metabolismo
Glicólise/efeitos dos fármacos
Seres Humanos
Queloide/tratamento farmacológico
Queloide/genética
Queloide/metabolismo
Ácido Láctico/metabolismo
Consumo de Oxigênio
Pele/metabolismo
Pele/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
33X04XA5AT (Lactic Acid); 9G2MP84A8W (Deoxyglucose); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180117
[St] Status:MEDLINE


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[PMID]:29214781
[Au] Autor:Kim B; Choi KM; Yim HS; Park HT; Yim JH; Lee MG
[Ad] Endereço:Department of Physiology, Korea University College of Medicine, Seoul, Korea.
[Ti] Título:Adipogenic and Lipolytic Effects of Ascorbic Acid in Ovariectomized Rats.
[So] Source:Yonsei Med J;59(1):85-91, 2018 Jan.
[Is] ISSN:1976-2437
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Ascorbic acid has been reported to have an adipogenic effect on 3T3-L1 preadipocytes, while evidence also suggests that ascorbic acid reduces body weight in humans. In this study, we tested the effects of ascorbic acid on adipogenesis and the balance of lipid accumulation in ovariectomized rats, in addition to long-term culture of differentiated 3T3-L1 adipocytes. MATERIALS AND METHODS: Murine 3T3-L1 fibroblasts and ovariectomized rats were treated with ascorbic acid at various time points. In vitro adipogenesis was analyzed by Oil Red O staining, and in vivo body fat was measured by a body composition analyzer using nuclear magnetic resonance. RESULTS: When ascorbic acid was applied during an early time point in 3T3-L1 preadipocyte differentiation and after bilateral ovariectomy (OVX) in rats, adipogenesis and fat mass gain significantly increased, respectively. However, lipid accumulation in well-differentiated 3T3-L1 adipocytes showed a significant reduction when ascorbic acid was applied after differentiation (10 days after induction). Also, oral ascorbic acid administration 4 weeks after OVX in rats significantly reduced both body weight and subcutaneous fat layer. In comparison to the results of ascorbic acid, which is a well-known cofactor for an enzyme of collagen synthesis, and the antioxidant ramalin, a potent antioxidant but not a cofactor, showed only a lipolytic effect in well-differentiated 3T3-L1 adipocytes, not an adipogenic effect. CONCLUSION: Taking these results into account, we concluded that ascorbic acid has both an adipogenic effect as a cofactor of an enzymatic process and a lipolytic effect as an antioxidant.
[Mh] Termos MeSH primário: Adipogenia/efeitos dos fármacos
Ácido Ascórbico/farmacologia
Lipólise/efeitos dos fármacos
Ovariectomia
[Mh] Termos MeSH secundário: Células 3T3-L1
Adipócitos/efeitos dos fármacos
Adipócitos/metabolismo
Animais
Antioxidantes/farmacologia
Composição Corporal/efeitos dos fármacos
Peso Corporal/efeitos dos fármacos
Diferenciação Celular/efeitos dos fármacos
Feminino
Fibroblastos/efeitos dos fármacos
Fibroblastos/metabolismo
Camundongos
Ratos Sprague-Dawley
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); PQ6CK8PD0R (Ascorbic Acid)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171208
[St] Status:MEDLINE
[do] DOI:10.3349/ymj.2018.59.1.85


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[PMID]:28470446
[Au] Autor:Sundaran PS; Bhaskaran A; Alex ST; Prasad T; Haritha VH; Anie Y; Kumary TV; Anil Kumar PR
[Ad] Endereço:Division of Tissue Culture, Department of Applied Biology, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala, 695 012, India.
[Ti] Título:Drug loaded microbeads entrapped electrospun mat for wound dressing application.
[So] Source:J Mater Sci Mater Med;28(6):88, 2017 Jun.
[Is] ISSN:1573-4838
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A new design of antibiotic loaded wound dressing and its initial in vitro evaluation is described. Chitosan microbeads loaded with ampicillin were sandwiched within polycaprolactone electrospun mat (MbAPPCL). The morphology was analyzed by scanning electron microscopy and surface chemistry was characterized by Fourier Transform Infrared Spectroscopy. In vitro cytotoxicity using L-929 fibroblast cells by direct contact test and elution assay revealed non-cytotoxic nature of MbAPPCL. The cell adhesion and viability analysis further confirmed the cytocompatibility of MbAPPCL as a wound dressing material. Percentage hemolysis and platelet adhesion on the mat exposed to blood substantiated the hemocompatibility. The antibiotic susceptibility test analyzed on Staphylococcus aureus by agar plate method confirmed the drug release and antimicrobial property. The proposed wound dressing model explained with ampicillin as a candidate drug has the potential to include microbeads with different antibiotics for multi drug treatment.
[Mh] Termos MeSH primário: Bandagens
Portadores de Fármacos
Microesferas
[Mh] Termos MeSH secundário: Animais
Antibacterianos/química
Antibacterianos/farmacologia
Materiais Biocompatíveis
Plaquetas
Linhagem Celular
Quitosana
Técnicas Eletroquímicas
Fibroblastos/fisiologia
Teste de Materiais
Camundongos
Penicilinas/química
Penicilinas/farmacologia
Staphylococcus aureus/efeitos dos fármacos
Estreptomicina/química
Estreptomicina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Biocompatible Materials); 0 (Drug Carriers); 0 (Penicillins); 9012-76-4 (Chitosan); Y45QSO73OB (Streptomycin)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1007/s10856-017-5893-8


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[PMID]:28457531
[Au] Autor:Kaufman G; Skrtic D
[Ad] Endereço:Volpe Research Center, ADA Foundation, Gaithersburg, MD 20899, USA. Electronic address: gili.kaufman@nist.gov.
[Ti] Título:Spatial development of gingival fibroblasts and dental pulp cells: Effect of extracellular matrix.
[So] Source:Tissue Cell;49(3):401-409, 2017 Jun.
[Is] ISSN:1532-3072
[Cp] País de publicação:Scotland
[La] Idioma:eng
[Ab] Resumo:Cells sensing changes in their microenvironmental stiffness and composition alter their responses, accordingly. This study determines whether gingival fibroblasts (GFs) and dental pulp mesenchymal stem cells (DPMSCs) support the formation of continuous layers in vitro by mimicking the stiffness and protein composition of their native extracellular matrix (ECM). Immortalized cells were incubated with (i) 0-100% Matrigel-ECM (M-ECM) for 7-28d, and with (ii) collagen and fibrin matrices for 14d. Cultures were analyzed by phase-contrast, fluorescence and confocal microscopies. The diameters and surface areas were measured via ImageJ. Self-renewal markers were detected by RT-PCR and immunocytochemistry assays. GFs and DPMSCs developed spheroids interconnected by elongated cell bundles or layers, respectively, expressing the self-renewal markers. Increased matrix stiffness resulted in spheroids replacement by the interconnecting cells/layers. Both cells required 100% M-ECM to reduce their spheroid diameter. However, it reduced the surface area of the interconnecting layers. Those differences led to extended, spindle-shaped GFs vs. compact, ring-shaped DPMSCs constructs. Collagen and fibrin matrices developed continuous layers of tightly connected cells vs. distinctive scattered cell aggregates, respectively. The ability of GFs and DPMSCs to create tissue-like multicellular layers at various matrix conditions may be imprinted by cells' adaptation to mechanical forces and composition in vivo.
[Mh] Termos MeSH primário: Polpa Dentária/metabolismo
Matriz Extracelular/química
Fibroblastos/metabolismo
Gengiva/metabolismo
[Mh] Termos MeSH secundário: Animais
Linhagem Celular Transformada
Polpa Dentária/citologia
Matriz Extracelular/metabolismo
Fibroblastos/citologia
Gengiva/citologia
Camundongos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


  5 / 108299 MEDLINE  
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[PMID]:28455252
[Au] Autor:Kwak HW; Shin M; Lee JY; Yun H; Song DW; Yang Y; Shin BS; Park YH; Lee KH
[Ad] Endereço:Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.
[Ti] Título:Fabrication of an ultrafine fish gelatin nanofibrous web from an aqueous solution by electrospinning.
[So] Source:Int J Biol Macromol;102:1092-1103, 2017 Sep.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Electrospinning of aqueous gelatin solution obtained from bovine or porcine sources has been difficult to achieve without additional facilities, such as a temperature control oven or heating cover. Gelatin from cold-water fish has low contents of proline (Pro) and hydroxyproline (Hyp) compared with mammalian-derived gelatin. For this reason, the fish-derived gelatin maintains a sol state without showing gelation behavior at room temperature. In the present study, we prepared an ultrafine fish gelatin nanofibrous web by electrospinning from aqueous solutions without any additive polymers or temperature control facilities. The concentration and viscosity of fish gelatin are the most important factor in determining the electrospinnability and fiber diameter. Electrospinning of aqueous fish gelatin has the highest nanofiber productivity compared to other organic solvent systems. Using glutaraldehyde vapor (GTA), the water stability was improved and substantial enhancement was achieved in the mechanical properties. Finally, the cytotoxicity of a fish gelatin nanofibrous scaffold was evaluated based on a cell proliferation study by culturing human dermal fibroblasts (HDFs) compared with a fish gelatin film and nanofibrous mat from mammalian gelatin. The result shows better initial cell attachment and proliferation compared with the fish gelatin film and no significant difference compared with mammalian-derived gelatin nanofibrous mat. We expect that electrospinning of aqueous fish gelatin could be an effective alternative mammalian gelatin source.
[Mh] Termos MeSH primário: Eletricidade
Peixes
Gelatina/química
Nanofibras/química
Nanotecnologia
Água/química
[Mh] Termos MeSH secundário: Animais
Materiais Biocompatíveis/química
Materiais Biocompatíveis/farmacologia
Bovinos
Adesão Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Fibroblastos/citologia
Fibroblastos/efeitos dos fármacos
Gelatina/farmacologia
Glutaral/química
Seres Humanos
Hidrólise
Reologia
Soluções
Viscosidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biocompatible Materials); 0 (Solutions); 059QF0KO0R (Water); 9000-70-8 (Gelatin); T3C89M417N (Glutaral)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE


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[PMID]:28452703
[Au] Autor:Shin JM; Park JH; Kim HJ; Park IH; Lee HM
[Ad] Endereço:Department of Otorhinolaryngology-Head and Neck Surgery, Korea University College of Medicine, Seoul, South Korea.
[Ti] Título:Cigarette smoke extract increases vascular endothelial growth factor production TLR4/ROS/MAPKs/NF-kappaB pathway in nasal fibroblast.
[So] Source:Am J Rhinol Allergy;31(2):78-84, 2017 Mar 01.
[Is] ISSN:1945-8932
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Cigarette smoke is a complex mixture of various chemical compounds, including free radicals and highly toxic compounds. Cigarette smoke exposure has been shown to be associated with chronic rhinosinusitis and tissue remodeling in upper airway. Vascular endothelial growth factor (VEGF) is one of the cytokines with a crucial role in tissue remodeling of airway. The aims of this study were to determine the effects of cigarette smoke extract (CSE) on VEGF expression and to investigate the underlying molecular mechanisms of CSE in nasal fibroblasts. METHODS: Nasal fibroblasts were stimulated with CSE. Cytotoxicity was evaluated by 3-(4,5- dimethylthiazol-2yl)-2,5-diphenyl-tetrazolium bromide assay. The expression level of VEGF was measured using reverse transcription-polymerase chain reaction (RT-PCR), and enzyme-linked immunosorbent assay. Messenger RNA (mRNA) expression level of TLR4 were determined by RT-PCR. Small interfering RNA (siRNA) for TLR4 was transfected to suppress TLR4 expression. Activation of reactive oxygen species (ROS) was analyzed by using dichloro-dihydro-fluorescein diacetate assay. Mitogen-activated protein kinase (MAPK) and NF-kappaB activations were determined by using western blot and/or luciferase assay. RESULTS: CSE had no significant cytotoxic effect in nasal fibroblast up to 5%. CSE significantly increased both VEGF mRNA and protein expression dose-dependently. The down-regulation of TLR4 transcription by siRNA treatment suppressed CSE-induced expressions of both TLR4 and VEGF. Pretreatment with ROS scavengers, specific inhibitors of each MAPK, and NF-kappaB inhibitor significantly decreased CSE-induced VEGF expression. CONCLUSIONS: CSE has a stimulatory effect on VEGF expression through the TLR4, ROS, MAPK, and NF-kappaB signaling pathway in nasal fibroblasts.
[Mh] Termos MeSH primário: Fibroblastos/fisiologia
Nariz/patologia
Receptor 4 Toll-Like/metabolismo
Fator A de Crescimento do Endotélio Vascular/metabolismo
[Mh] Termos MeSH secundário: Adulto
Morte Celular
Células Cultivadas
Fumar Cigarros/efeitos adversos
MAP Quinases Reguladas por Sinal Extracelular/metabolismo
Feminino
Regulação da Expressão Gênica
Seres Humanos
Masculino
NF-kappa B/metabolismo
RNA Interferente Pequeno/genética
Espécies Reativas de Oxigênio/metabolismo
Transdução de Sinais
Receptor 4 Toll-Like/genética
Fator A de Crescimento do Endotélio Vascular/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (NF-kappa B); 0 (RNA, Small Interfering); 0 (Reactive Oxygen Species); 0 (TLR4 protein, human); 0 (Toll-Like Receptor 4); 0 (Vascular Endothelial Growth Factor A); EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.2500/ajra.2017.31.4415


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[PMID]:29458673
[Au] Autor:Morse DJ; Wilson MJ; Wei X; Lewis MAO; Bradshaw DJ; Murdoch C; Williams DW
[Ad] Endereço:1​Oral and Biomedical Sciences, School of Dentistry, Cardiff University, Cardiff, UK.
[Ti] Título:Denture-associated biofilm infection in three-dimensional oral mucosal tissue models.
[So] Source:J Med Microbiol;67(3):364-375, 2018 Mar.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: In vitro analyses of virulence, pathogenicity and associated host cell responses are important components in the study of biofilm infections. The Candida-related infection, denture-associated oral candidosis, affects up to 60 % of denture wearers and manifests as inflammation of palatal tissues contacting the denture-fitting surface. Commercially available three-dimensional tissue models can be used to study infection, but their use is limited for many academic research institutions, primarily because of the substantial purchase costs. The aim of this study was to develop and evaluate the use of in vitro tissue models to assess infections by biofilms on acrylic surfaces through tissue damage and Candida albicans virulence gene expression. METHODOLOGY: In vitro models were compared against commercially available tissue equivalents (keratinocyte-only, SkinEthic; full-thickness, MatTek Corporation). An in vitro keratinocyte-only tissue was produced using a cancer-derived cell line, TR146, and a full-thickness model incorporating primary fibroblasts and immortalised normal oral keratinocytes was also generated. The in vitro full-thickness tissues incorporated keratinocytes and fibroblasts, and have potential for future further development and analysis. RESULTS: Following polymicrobial infection with biofilms on acrylic surfaces, both in-house developed models were shown to provide equivalent results to the SkinEthic and MatTek models in terms of tissue damage: a significant (P<0.05) increase in LDH activity for mixed species biofilms compared to uninfected control, and no significant difference (P>0.05) in the expression of most C. albicans virulence genes when comparing tissue models of the same type. CONCLUSION: Our results confirm the feasibility and suitability of using these alternative in vitro tissue models for such analyses.
[Mh] Termos MeSH primário: Biofilmes/crescimento & desenvolvimento
Candidíase Bucal/microbiologia
Dentaduras/microbiologia
Interações Hospedeiro-Patógeno
Mucosa Bucal/microbiologia
[Mh] Termos MeSH secundário: Candida albicans/genética
Candida albicans/patogenicidade
Candida albicans/fisiologia
Linhagem Celular
Coinfecção/microbiologia
Fibroblastos/microbiologia
Seres Humanos
Queratinócitos/microbiologia
Polimetil Metacrilato
Estomatite sob Prótese
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9011-14-7 (Polymethyl Methacrylate)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000677


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[PMID]:28463419
[Au] Autor:Lin C; Khetani SR
[Ad] Endereço:School of Biomedical Engineering, Colorado State University, Fort Collins, Colorado.
[Ti] Título:Micropatterned Co-Cultures of Human Hepatocytes and Stromal Cells for the Assessment of Drug Clearance and Drug-Drug Interactions.
[So] Source:Curr Protoc Toxicol;72:14.17.1-14.17.23, 2017 May 02.
[Is] ISSN:1934-9262
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Drug clearance rates from the body can determine drug exposure that can affect efficacy or toxicity. Thus, accurate prediction of drug clearance during preclinical development can help guide dose selection in humans, but animal testing is not always predictive of human outcomes. Because hepatic drug metabolism is a rate-limiting step in the overall clearance of many drugs, primary human hepatocytes (PHHs) in suspension cultures or monolayers are used for drug clearance predictions. Yet, the precipitous decline in drug metabolism capacity can lead to significant underestimation of clearance rates, particularly for low turnover compounds that have desirable one-pill-a-day dosing regimens. In contrast, micropatterned co-cultures (MPCCs) of PHHs and fibroblasts display phenotypic stability for several weeks and can help mitigate the limitations of conventional cultures. Here, we describe protocols to create and use MPCCs for drug clearance predictions, and for modeling clinically-relevant drug-drug interactions that can affect drug clearance. © 2017 by John Wiley & Sons, Inc.
[Mh] Termos MeSH primário: Técnicas de Cocultura/métodos
Interações Medicamentosas
Hepatócitos/metabolismo
Preparações Farmacêuticas/metabolismo
Células Estromais/metabolismo
[Mh] Termos MeSH secundário: Células 3T3
Animais
Técnicas de Cultura de Células
Meios de Cultura
Fibroblastos/metabolismo
Hepatócitos/ultraestrutura
Seres Humanos
Taxa de Depuração Metabólica
Camundongos
Fenótipo
Células Estromais/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media); 0 (Pharmaceutical Preparations)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1002/cptx.23


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[PMID]:29412224
[Au] Autor:El-Batal AI; Ahmed SF
[Ad] Endereço:National Centre for Radiation Research and Technology - NCRRT, Atomic Energy Authority, Drug Radiation Research Department, Nasr City, Cairo, Egypt.
[Ti] Título:Therapeutic effect of Aloe vera and silver nanoparticles on acid-induced oral ulcer in gamma-irradiated mice.
[So] Source:Braz Oral Res;32:e004, 2018 Feb 05.
[Is] ISSN:1807-3107
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Radiation combined injury, a life-threatening condition, has higher mortality than simple radiation injury. The aim of the present study was to analyze the efficiency of Aloe vera and silver nanoparticles in improving the healing of ulcerated oral mucosa after irradiation. Thirty male Albino mice were divided into five groups: control, radiation, Aloe vera (AV), silver nanoparticles (NS), and AV+NS. The mice were exposed to whole body 6Gy gamma-radiation. After one hour, 20% acetic acid was injected into the submucosal layer of the lower lip for ulcer induction. The animals received topical treatment with the assigned substances for 5 days. Lip specimens were subjected to hematoxylin and eosin and anti alpha-smooth muscle actin immunohistochemical staining. Results demonstrated occurance of ulcer three days post irradiation in all groups except in the AV+NS group where only epithelial detachment was developed. After seven days, data revealed persistent ulcer in radiation group, and almost normal epithelium in the AV+NS group. A significant reduction of epithelial thickness was detected in all groups at the third day as compared to control. At the seventh day, only the AV+NS group restored the epithelial thickness. Area percent of alpha-smooth muscle actin expression was significantly decreased in radiation group at the third day followed by significant increase at the seventh day. However, all treatment groups showed significant increase in alpha-smooth muscle actin at the third day, which decreased to normal level at the seventh day. Our study demonstrated the efficiency of Aloe vera and silver nanoparticles in enhancing ulcer healing after irradiation.
[Mh] Termos MeSH primário: Aloe/química
Raios gama/efeitos adversos
Nanopartículas Metálicas/uso terapêutico
Úlceras Orais/tratamento farmacológico
Úlceras Orais/etiologia
Lesões Experimentais por Radiação/tratamento farmacológico
Prata/uso terapêutico
[Mh] Termos MeSH secundário: Ácido Acético
Actinas/análise
Administração Tópica
Animais
Células Epiteliais/efeitos dos fármacos
Células Epiteliais/efeitos da radiação
Fibroblastos/efeitos dos fármacos
Imuno-Histoquímica
Masculino
Camundongos
Microscopia Eletrônica de Transmissão
Úlceras Orais/patologia
Lesões Experimentais por Radiação/patologia
Valores de Referência
Reprodutibilidade dos Testes
Fatores de Tempo
Resultado do Tratamento
Cicatrização/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Actins); 0 (alpha-smooth muscle actin, mouse); 3M4G523W1G (Silver); Q40Q9N063P (Acetic Acid)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:180208
[St] Status:MEDLINE


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[PMID]:28455143
[Au] Autor:Li H; Li B; Larose L
[Ad] Endereço:Department of Medicine, McGill University, Montreal, QC H4A 3J1, Canada; The Research Institute of McGill University Health Centre, Montreal, QC H4A 3J1, Canada.
[Ti] Título:IRE1α links Nck1 deficiency to attenuated PTP1B expression in HepG2 cells.
[So] Source:Cell Signal;36:79-90, 2017 Aug.
[Is] ISSN:1873-3913
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PTP1B, a prototype of the non-receptor subfamily of the protein tyrosine phosphatase superfamily, plays a key role in regulating intracellular signaling from various receptor and non-receptor protein tyrosine kinases. Previously, we reported that silencing Nck1 in human hepatocellular carcinoma HepG2 cells enhances basal and growth factor-induced activation of the PI3K-Akt pathway through attenuating PTP1B expression. However, the underlying mechanism by which Nck1 depletion represses PTP1B expression remains unclear. In this study, we found that silencing Nck1 attenuates PTP1B expression in HepG2 cells through down-regulation of IRE1α. Indeed, we show that silencing Nck1 in HepG2 cells leads to decreased IRE1α expression and signaling. Accordingly, IRE1α depletion using siRNA in HepG2 cells enhances PI3K-dependent basal and growth factor-induced Akt activation, reproducing the effects of silencing Nck1 on activation of this pathway. In addition, depletion of IRE1α also leads to reduced PTP1B expression, which was rescued by ectopic expression of IRE1α in Nck1-depleted cells. Mechanistically, we found that silencing either Nck1 or IRE1α in HepG2 cells decreases PTP1B mRNA levels and stability. However, despite miR-122 levels, a miRNA targeting PTP1B 3' UTR and inducing PTP1B mRNA degradation in HepG2 cells, are increased in both Nck1- and IRE1α-depleted HepG2 cells, a miR-122 antagomir did not rescue PTP1B expression in these cells. Overall, this study highlights an important role for Nck1 in fine-tuning IRE1α expression and signaling that regulate PTP1B expression and subsequent activation of the PI3K-Akt pathway in HepG2 cells.
[Mh] Termos MeSH primário: Proteínas Adaptadoras de Transdução de Sinal/deficiência
Proteínas Adaptadoras de Transdução de Sinal/metabolismo
Endorribonucleases/metabolismo
Proteínas Oncogênicas/deficiência
Proteínas Oncogênicas/metabolismo
Proteína Tirosina Fosfatase não Receptora Tipo 1/metabolismo
Proteínas Serina-Treonina Quinases/metabolismo
[Mh] Termos MeSH secundário: Proteínas Adaptadoras de Transdução de Sinal/química
Animais
Ativação Enzimática/efeitos dos fármacos
Fibroblastos/efeitos dos fármacos
Fibroblastos/metabolismo
Inativação Gênica/efeitos dos fármacos
Células HeLa
Células Hep G2
Seres Humanos
Camundongos
MicroRNAs/metabolismo
Proteínas Oncogênicas/química
Fosfatidilinositol 3-Quinases/metabolismo
Fosforilação/efeitos dos fármacos
Ligação Proteica/efeitos dos fármacos
Domínios Proteicos
Proteína Tirosina Fosfatase não Receptora Tipo 1/genética
Proteínas Proto-Oncogênicas c-akt/metabolismo
Estabilidade de RNA/efeitos dos fármacos
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Transdução de Sinais/efeitos dos fármacos
Tapsigargina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adaptor Proteins, Signal Transducing); 0 (MIRN122 microRNA, human); 0 (MicroRNAs); 0 (Nck protein); 0 (Oncogene Proteins); 0 (RNA, Messenger); 67526-95-8 (Thapsigargin); EC 2.7.1.- (Phosphatidylinositol 3-Kinases); EC 2.7.11.1 (ERN1 protein, human); EC 2.7.11.1 (Protein-Serine-Threonine Kinases); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt); EC 3.1.- (Endoribonucleases); EC 3.1.3.48 (PTPN1 protein, human); EC 3.1.3.48 (Protein Tyrosine Phosphatase, Non-Receptor Type 1)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE



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