Base de dados : MEDLINE
Pesquisa : A11.329.629 [Categoria DeCS]
Referências encontradas : 26766 [refinar]
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[PMID]:29191879
[Au] Autor:Engblom C; Pfirschke C; Zilionis R; Da Silva Martins J; Bos SA; Courties G; Rickelt S; Severe N; Baryawno N; Faget J; Savova V; Zemmour D; Kline J; Siwicki M; Garris C; Pucci F; Liao HW; Lin YJ; Newton A; Yaghi OK; Iwamoto Y; Tricot B; Wojtkiewicz GR; Nahrendorf M; Cortez-Retamozo V; Meylan E; Hynes RO; Demay M; Klein A; Bredella MA; Scadden DT; Weissleder R; Pittet MJ
[Ad] Endereço:Center for Systems Biology, Massachusetts General Hospital Research Institute and Harvard Medical School, Boston, MA 02114, USA.
[Ti] Título:Osteoblasts remotely supply lung tumors with cancer-promoting SiglecF neutrophils.
[So] Source:Science;358(6367), 2017 12 01.
[Is] ISSN:1095-9203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bone marrow-derived myeloid cells can accumulate within tumors and foster cancer outgrowth. Local immune-neoplastic interactions have been intensively investigated, but the contribution of the systemic host environment to tumor growth remains poorly understood. Here, we show in mice and cancer patients ( = 70) that lung adenocarcinomas increase bone stromal activity in the absence of bone metastasis. Animal studies reveal that the cancer-induced bone phenotype involves bone-resident osteocalcin-expressing (Ocn ) osteoblastic cells. These cells promote cancer by remotely supplying a distinct subset of tumor-infiltrating SiglecF neutrophils, which exhibit cancer-promoting properties. Experimentally reducing Ocn cell numbers suppresses the neutrophil response and lung tumor outgrowth. These observations posit osteoblasts as remote regulators of lung cancer and identify SiglecF neutrophils as myeloid cell effectors of the osteoblast-driven protumoral response.
[Mh] Termos MeSH primário: Adenocarcinoma/patologia
Antígenos CD/metabolismo
Antígenos de Diferenciação Mielomonocítica/metabolismo
Osso e Ossos/patologia
Lectinas/metabolismo
Neoplasias Pulmonares/patologia
Infiltração de Neutrófilos
Neutrófilos/metabolismo
Neutrófilos/patologia
Osteoblastos/patologia
[Mh] Termos MeSH secundário: Animais
Densidade Óssea
Células da Medula Óssea/patologia
Osso e Ossos/metabolismo
Linhagem Celular Tumoral
Seres Humanos
Camundongos
Camundongos Endogâmicos C57BL
Células Mieloides/patologia
Neoplasias Experimentais/patologia
Osteocalcina/metabolismo
Receptor para Produtos Finais de Glicação Avançada/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antigens, CD); 0 (Antigens, Differentiation, Myelomonocytic); 0 (Lectins); 0 (Receptor for Advanced Glycation End Products); 0 (SIGLEC5 protein, human); 0 (sRAGE protein, human); 104982-03-8 (Osteocalcin)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171202
[St] Status:MEDLINE


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[PMID]:29408431
[Au] Autor:Li Y; Hu W; Han G; Lu W; Jia D; Hu M; Wang D
[Ad] Endereço:Department of Orthodontics, School and Hospital of Stomatology, Jilin University, Changchun 130021, China; Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Changchun 130021, China. Electronic address: m18643107595@163.com.
[Ti] Título:Involvement of bone morphogenetic protein-related pathways in the effect of aucubin on the promotion of osteoblast differentiation in MG63 cells.
[So] Source:Chem Biol Interact;283:51-58, 2018 Mar 01.
[Is] ISSN:1872-7786
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Aucubin, an iridoid glycoside found in several plants, such as Eucommia ulmoide and Rehmannia, has various pharmacological effects. Bone formation is a complex process in which osteoblast differentiation plays an important role. This study aimed to investigate the promotion effects of aucubin on osteoblast differentiation in MG63 cells, a human osteoblast-like cell line. Aucubin not only improved osteoblast differentiation, as shown by enhanced ALP (alkaline phosphatase) concentration and mineralization in cells, but increased the expression of various cytokines, including collagen I, osteocalcin, osteopontin, integrin ß1, and Osterix. Aucubin strongly enhanced the levels of BMP2 (bone morphogenetic proteins-2) in MG63 cells, which play a central role during osteoblast differentiation. Further data show that aucubin exposure after 1 day, 7 days, and 14 days enhanced the expression of Smad1, 5, and 8, and the phosphoresced levels of MAPKs (mitogen-activated protein kinases) family Erk (extracellular signal-regulated kinases), JNK (c-Jun-NH2-terminal kinases), P38, and Akt (serine/threonine protein kinase)/mTOR (mammalian target of rapamycin)/p70s6k in MG63 cells. This study shows the improved effects of aucubin on osteoblast differentiation in MG63 cells, related to the signaling of BMP2-mediated Smads (drosophila mothers against decapentaplegic proteins), MAPKs, and Akt/mTOR/p70S6K. This study indicates the potential of aucubin for osteoporosis treatment.
[Mh] Termos MeSH primário: Proteína Morfogenética Óssea 2/metabolismo
Diferenciação Celular/efeitos dos fármacos
Glucosídeos Iridoides/farmacologia
[Mh] Termos MeSH secundário: Linhagem Celular
Proliferação Celular/efeitos dos fármacos
Colágeno Tipo I/metabolismo
MAP Quinases Reguladas por Sinal Extracelular/metabolismo
Seres Humanos
Glucosídeos Iridoides/química
Proteínas Quinases JNK Ativadas por Mitógeno/metabolismo
Sistema de Sinalização das MAP Quinases/efeitos dos fármacos
Osteoblastos/citologia
Osteoblastos/efeitos dos fármacos
Osteoblastos/metabolismo
Osteocalcina/metabolismo
Osteopontina/metabolismo
Fosforilação/efeitos dos fármacos
Proteínas Proto-Oncogênicas c-akt/metabolismo
Proteína Smad1/metabolismo
Proteína Smad5/metabolismo
Proteína Smad8/metabolismo
Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (BMP2 protein, human); 0 (Bone Morphogenetic Protein 2); 0 (Collagen Type I); 0 (Iridoid Glucosides); 0 (Smad1 Protein); 0 (Smad5 Protein); 0 (Smad8 Protein); 104982-03-8 (Osteocalcin); 106441-73-0 (Osteopontin); 2G52GS8UML (aucubin); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt); EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases); EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases); EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180207
[St] Status:MEDLINE


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[PMID]:28464904
[Au] Autor:Bardeesi ASA; Gao J; Zhang K; Yu S; Wei M; Liu P; Huang H
[Ad] Endereço:Zhongshan Medical School, Sun Yat-sen University, Guangzhou, China.
[Ti] Título:A novel role of cellular interactions in vascular calcification.
[So] Source:J Transl Med;15(1):95, 2017 May 03.
[Is] ISSN:1479-5876
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A series of clinical trials have confirmed the correlation between vascular calcification (VC) and cardiovascular events and mortality. However, current treatments have little effects on the regression of VC. Potent and illustrative mechanisms have been proven to exist in both bone metabolism and VC, indicating that these two processes share similarities in onset and progression. Multiple osteoblast-like cells and signaling pathways are involved in the process of VC. In this review, we summarized the roles of different osteoblast-like cells and we emphasized on how they communicated and interacted with each other using different signaling pathways. Further studies are needed to uncover the underlying mechanisms and to provide novel therapies for VC.
[Mh] Termos MeSH primário: Comunicação Celular
Calcificação Vascular/patologia
[Mh] Termos MeSH secundário: Animais
Células Endoteliais/patologia
Seres Humanos
Osteoblastos/patologia
Transdução de Sinais
Células-Tronco/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1186/s12967-017-1190-z


  4 / 26766 MEDLINE  
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[PMID]:29176792
[Au] Autor:Tai PWL; Wu H; van Wijnen AJ; Stein GS; Stein JL; Lian JB
[Ad] Endereço:Department of Biochemistry, University of Vermont College of Medicine, Burlington, Vermont, United States of America.
[Ti] Título:Genome-wide DNase hypersensitivity, and occupancy of RUNX2 and CTCF reveal a highly dynamic gene regulome during MC3T3 pre-osteoblast differentiation.
[So] Source:PLoS One;12(11):e0188056, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The ability to discover regulatory sequences that control bone-related genes during development has been greatly improved by massively parallel sequencing methodologies. To expand our understanding of cis-regulatory regions critical to the control of gene expression during osteoblastogenesis, we probed the presence of open chromatin states across the osteoblast genome using global DNase hypersensitivity (DHS) mapping. Our profiling of MC3T3 mouse pre-osteoblasts during differentiation has identified more than 224,000 unique DHS sites. Approximately 65% of these sites are dynamic during temporal stages of osteoblastogenesis, and a majority of them are located within non-promoter (intergenic and intronic) regions. Nearly half of all DHS sites (both constitutive and dynamic) overlap binding events of the bone-essential RUNX2 and/or the chromatin-related CTCF transcription factors. This finding reinforces the role of these regulatory proteins as essential components of the bone gene regulome. We observe a reduction in chromatin accessibility throughout the genome between pre-osteoblast and early osteoblasts. Our analysis also defined a class of differentially expressed genes that harbor DHS peaks centered within 1 kb downstream of transcriptional end sites (TES). These DHSs at the 3'-flanks of genes exhibit dynamic changes during differentiation that may impact regulation of the osteoblast genome. Taken together, the distribution of DHS regions within non-promoter locations harboring osteoblast and chromatin related transcription factor binding motifs, reflect novel cis-regulatory requirements to support temporal gene expression in differentiating osteoblasts.
[Mh] Termos MeSH primário: Fator de Ligação a CCCTC/metabolismo
Diferenciação Celular/genética
Subunidade alfa 1 de Fator de Ligação ao Core/metabolismo
Desoxirribonucleases/metabolismo
Regulação da Expressão Gênica
Genoma
Osteoblastos/citologia
Osteoblastos/metabolismo
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
DNA Intergênico/genética
Perfilação da Expressão Gênica
Ontologia Genética
Íntrons/genética
Camundongos
Ativação Transcricional/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CCCTC-Binding Factor); 0 (Core Binding Factor Alpha 1 Subunit); 0 (DNA, Intergenic); EC 3.1.- (Deoxyribonucleases)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188056


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[PMID]:28453953
[Au] Autor:Ramaraju H; Miller SJ; Kohn DH
[Ad] Endereço:Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA.
[Ti] Título:Dual-functioning peptides discovered by phage display increase the magnitude and specificity of BMSC attachment to mineralized biomaterials.
[So] Source:Biomaterials;134:1-12, 2017 Jul.
[Is] ISSN:1878-5905
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Design of biomaterials for cell-based therapies requires presentation of specific physical and chemical cues to cells, analogous to cues provided by native extracellular matrices (ECM). We previously identified a peptide sequence with high affinity towards apatite (VTKHLNQISQSY, VTK) using phage display. The aims of this study were to identify a human MSC-specific peptide sequence through phage display, combine it with the apatite-specific sequence, and verify the specificity of the combined dual-functioning peptide to both apatite and human bone marrow stromal cells. In this study, a combinatorial phage display identified the cell binding sequence (DPIYALSWSGMA, DPI) which was combined with the mineral binding sequence to generate the dual peptide DPI-VTK. DPI-VTK demonstrated significantly greater binding affinity (1/K ) to apatite surfaces compared to VTK, phosphorylated VTK (VTK ), DPI-VTK , RGD-VTK, and peptide-free apatite surfaces (p < 0.01), while significantly increasing hBMSC adhesion strength (τ , p < 0.01). MSCs demonstrated significantly greater adhesion strength to DPI-VTK compared to other cell types, while attachment of MC3T3 pre-osteoblasts and murine fibroblasts was limited (p < 0.01). MSCs on DPI-VTK coated surfaces also demonstrated increased spreading compared to pre-osteoblasts and fibroblasts. MSCs cultured on DPI-VTK coated apatite films exhibited significantly greater proliferation compared to controls (p < 0.001). Moreover, early and late stage osteogenic differentiation markers were elevated on DPI-VTK coated apatite films compared to controls. Taken together, phage display can identify non-obvious cell and material specific peptides to increase human MSC adhesion strength to specific biomaterial surfaces and subsequently increase cell proliferation and differentiation. These new peptides expand biomaterial design methodology for cell-based regeneration of bone defects. This strategy of combining cell and material binding phage display derived peptides is broadly applicable to a variety of systems requiring targeted adhesion of specific cell populations, and may be generalized to the engineering of any adhesion surface.
[Mh] Termos MeSH primário: Materiais Biocompatíveis/química
Células Mesenquimais Estromais/citologia
Peptídeos/farmacologia
[Mh] Termos MeSH secundário: Animais
Materiais Biomiméticos/química
Adesão Celular/efeitos dos fármacos
Adesão Celular/fisiologia
Diferenciação Celular/efeitos dos fármacos
Diferenciação Celular/fisiologia
Células Cultivadas
Fibroblastos/citologia
Fibroblastos/efeitos dos fármacos
Fibroblastos/fisiologia
Seres Humanos
Células Mesenquimais Estromais/efeitos dos fármacos
Células Mesenquimais Estromais/fisiologia
Osteoblastos/citologia
Osteoblastos/efeitos dos fármacos
Osteoblastos/fisiologia
Peptídeos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biocompatible Materials); 0 (Peptides)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE


  6 / 26766 MEDLINE  
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[PMID]:29352112
[Au] Autor:Furuya M; Kikuta J; Fujimori S; Seno S; Maeda H; Shirazaki M; Uenaka M; Mizuno H; Iwamoto Y; Morimoto A; Hashimoto K; Ito T; Isogai Y; Kashii M; Kaito T; Ohba S; Chung UI; Lichtler AC; Kikuchi K; Matsuda H; Yoshikawa H; Ishii M
[Ad] Endereço:Department of Immunology and Cell Biology, Graduate School of Medicine & Frontier Biosciences, Osaka University, Osaka, 565-0871, Japan.
[Ti] Título:Direct cell-cell contact between mature osteoblasts and osteoclasts dynamically controls their functions in vivo.
[So] Source:Nat Commun;9(1):300, 2018 01 19.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bone homeostasis is regulated by communication between bone-forming mature osteoblasts (mOBs) and bone-resorptive mature osteoclasts (mOCs). However, the spatial-temporal relationship and mode of interaction in vivo remain elusive. Here we show, by using an intravital imaging technique, that mOB and mOC functions are regulated via direct cell-cell contact between these cell types. The mOBs and mOCs mainly occupy discrete territories in the steady state, although direct cell-cell contact is detected in spatiotemporally limited areas. In addition, a pH-sensing fluorescence probe reveals that mOCs secrete protons for bone resorption when they are not in contact with mOBs, whereas mOCs contacting mOBs are non-resorptive, suggesting that mOBs can inhibit bone resorption by direct contact. Intermittent administration of parathyroid hormone causes bone anabolic effects, which lead to a mixed distribution of mOBs and mOCs, and increase cell-cell contact. This study reveals spatiotemporal intercellular interactions between mOBs and mOCs affecting bone homeostasis in vivo.
[Mh] Termos MeSH primário: Reabsorção Óssea/diagnóstico por imagem
Comunicação Celular/fisiologia
Osteoblastos/citologia
Osteoclastos/citologia
Osteogênese/fisiologia
[Mh] Termos MeSH secundário: Animais
Diferenciação Celular
Feminino
Corantes Fluorescentes/química
Expressão Gênica
Genes Reporter
Proteínas de Fluorescência Verde/genética
Proteínas de Fluorescência Verde/metabolismo
Homeostase/fisiologia
Concentração de Íons de Hidrogênio
Microscopia Intravital/métodos
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Transgênicos
Osteoblastos/efeitos dos fármacos
Osteoblastos/fisiologia
Osteoclastos/efeitos dos fármacos
Osteoclastos/fisiologia
Hormônio Paratireóideo/farmacologia
Cultura Primária de Células
Crânio/citologia
Crânio/diagnóstico por imagem
Crânio/efeitos dos fármacos
Crânio/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fluorescent Dyes); 0 (Parathyroid Hormone); 0 (enhanced green fluorescent protein); 147336-22-9 (Green Fluorescent Proteins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180121
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02541-w


  7 / 26766 MEDLINE  
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[PMID]:29305866
[Au] Autor:Zhang W; Xu J; Qiu J; Xing C; Li X; Leng B; Su Y; Lin J; Lin J; Mei X; Huang Y; Pan Y; Xue Y
[Ad] Endereço:The Engineering Technological Center of Mushroom Industry, Minnan Normal University, Zhangzhou, Fujian, 363000, China.
[Ti] Título:Novel and rapid osteoporosis model established in zebrafish using high iron stress.
[So] Source:Biochem Biophys Res Commun;496(2):654-660, 2018 02 05.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Osteoporosis is a global public health concern and, it can result from numerous pathogenic mechanisms, many of which are closely related with age, nutritional disorders, endocrine imbalance, or adverse drug side effects presented by glucocorticoids, heparin, and anti-epileptics. Given its wide range etiologies, it is crucial to establish an animal model of osteoporosis for use in screening potential drugs quickly and effectively. Previous research has reported that an accumulation of elevated iron in the body is an independent risk factor for osteoporosis. As such, we sought to use both zebrafish larvae and adults to model an osteoporosis phenotype using high iron stress (FAC, ferric ammonium citrate). Skeletal staining results suggested that iron-overload caused a significant decrease in bone calcification as well as severe developmental cartilage defects. In addition, osteoblast and cartilage-specific mRNA expression levels were downregulated after exposure to a high-iron environment. Most importantly, we demonstrated in both larval and adult fish that high iron-induced osteogenic defects were significantly rescued using alendronate (AL), a drug known to be effective against to human osteoporosis. Even more, the repair effect of AL was achieved by facilitating osteoblast differentiation and targeting Bmp signaling. Taken together, our findings propose an rapid and effective osteoporosis model, which could be used widely for future osteoporosis drug screening.
[Mh] Termos MeSH primário: Osso e Ossos/patologia
Sobrecarga de Ferro/metabolismo
Osteoblastos/patologia
Osteoporose/metabolismo
Peixe-Zebra
[Mh] Termos MeSH secundário: Alendronato/uso terapêutico
Animais
Conservadores da Densidade Óssea/uso terapêutico
Osso e Ossos/efeitos dos fármacos
Osso e Ossos/metabolismo
Calcificação Fisiológica/efeitos dos fármacos
Modelos Animais de Doenças
Ferro/metabolismo
Sobrecarga de Ferro/tratamento farmacológico
Sobrecarga de Ferro/patologia
Sobrecarga de Ferro/fisiopatologia
Osteoblastos/efeitos dos fármacos
Osteoblastos/metabolismo
Osteogênese/efeitos dos fármacos
Osteoporose/tratamento farmacológico
Osteoporose/patologia
Osteoporose/fisiopatologia
Peixe-Zebra/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bone Density Conservation Agents); E1UOL152H7 (Iron); X1J18R4W8P (Alendronate)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180107
[St] Status:MEDLINE


  8 / 26766 MEDLINE  
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[PMID]:29305863
[Au] Autor:Qu B; Gong K; Yang HS; Li YG; Jiang T; Zeng ZM; Cao ZR; Pan XM
[Ad] Endereço:Department of Orthopaedics, The First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan Province, China; Center for Disease Control and Prevention of the Chengdu Military Command, Chengdu, Sichuan Province, China.
[Ti] Título:MiR-449 overexpression inhibits osteogenic differentiation of bone marrow mesenchymal stem cells via suppressing Sirt1/Fra-1 pathway in high glucose and free fatty acids microenvironment.
[So] Source:Biochem Biophys Res Commun;496(1):120-126, 2018 01 29.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Diabetic osteoporosis is a chronic complication caused by diabetes mellitus, and However, the exact mechanism of diabetes mellitus-induced osteoporosis is still unknown. In this study, we investigate the effect of miR-449 on osteogenic differentiation and its underlying mechanism in human bone marrow-derived mesenchymal stem cells (hBMSCs) with high glucose (HG) and free fatty acids (FFA) treatment. Results showed that after culturing for 14 days, high glucose (HG) and free fatty acids (FFA) treatment dramatically decreased mineralization of human bone marrow-derived mesenchymal stem cells (hBMSCs) compared with cells treated with osteogenic medium (OM) alone. We also found that miR-449 expression was up-regulated during osteogenic differentiation of hBMSCs with HG and FFA treatment. Moreover, during osteogenic differentiation of hBMSCs with HG and FFA treatment, miR-449 mimics notably decreased the alkaline phosphatase (ALP) activity and the mRNA and protein expression levels of runt-related transcription factor 2 (Runx2), ALP, collagen I, osteocalcin (OCN), and bone sialoprotein (BSP), which was remarkably increased by miR-449 inhibitors. Furthermore, miR-449 directly targets Sirt1 by binding to its 3'-UTR. Sirt1 overexpression reverses the suppressive effect of miR-449 mimics on Fra-1 mRNA and protein expression, which was also alleviated by Fra-1 overexpression. In addition, Fra-1 overexpression alleviates the inhibitory effect of miR-449 mimics on the ALP activity and the mRNA and protein of Runx2, collagen I, OCN and BSP. Taken together, our results indicated that miR-449 overexpression inhibited osteogenic differentiation of HG-FFA-treated hBMSCs through the Sirt1/Fra-1 signal pathway. It is conceivable that modulating miR-449 might provide a new therapy for intervention in diabetic osteoporosis.
[Mh] Termos MeSH primário: Ácidos Graxos não Esterificados/metabolismo
Glucose/metabolismo
Células Mesenquimais Estromais/citologia
Células Mesenquimais Estromais/fisiologia
Osteogênese/fisiologia
Proteínas Proto-Oncogênicas c-fos/metabolismo
Sirtuína 1/metabolismo
[Mh] Termos MeSH secundário: Diferenciação Celular/fisiologia
Células Cultivadas
Seres Humanos
Osteoblastos/citologia
Osteoblastos/fisiologia
Transdução de Sinais/fisiologia
Nicho de Células-Tronco/fisiologia
Regulação para Cima/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fatty Acids, Nonesterified); 0 (Proto-Oncogene Proteins c-fos); 0 (fos-related antigen 1); EC 3.5.1.- (SIRT1 protein, human); EC 3.5.1.- (Sirtuin 1); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180107
[St] Status:MEDLINE


  9 / 26766 MEDLINE  
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[PMID]:28452332
[Au] Autor:Wang X; Chen W; Liu Q; Gao K; Wang G; Gao L; Liu L
[Ad] Endereço:Department of Chemistry and Biology, College of Science, National University of Defense Technology, Changsha, Hunan, 410073, People's Republic of China.
[Ti] Título:Function and mechanism of mesoporous bioactive glass adsorbed epidermal growth factor for accelerating bone tissue regeneration.
[So] Source:Biomed Mater;12(2):025020, 2017 Apr 28.
[Is] ISSN:1748-605X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Mesoporous bioactive glass (MBG) has been demonstrated to play a vital role in bone tissue engineering due to its bioactivity, biocompatibility, and osteoinduction properties. Here, we report that MBG grafted with an amino group (MBG-NH ) and MBG-NH adsorbed epidermal growth factor (EGF) (MBG-NH /EGF) sustained-release EGF, and MBG-NH2/EGF could accelerate osteoblast differentiation and mineralization in MC3T3-E1 cells. We found that MBG-NH could promote bone-like deposit formation and Ca deposition in vitro. Intriguingly, we observed that MBG-NH /EGF enhanced MC3T3-E1 cell adhesion. We also showed that extracellular signal-regulated kinase 1/2 (ERK1/2) was phosphorylated when MC3T3-E1 cells were cultured on MBG-NH /EGF. Interestingly, the transcription factor Runx2, important for osteoblast differentiation, was also activated when MC3T3-E1 cells were cultured on MBG-NH /EGF. We showed that MC3T3-E1 cells cultured on MBG-NH /EGF activating Runx2 was through ERK1/2 phosphorylation. Consistent with this survey, we observed that MC3T3-E1 cells cultured on MBG-NH /EGF accelerated osteoblastic marker gene expressions, including osteopontin (Opn) and osteocalcin (Ocn). Taken together, we conclude that the osteoblast differentiation and mineralization were accelerated in MC3T3-E1 cells cultured on MBG-NH /EGF through ERK-activated Runx2 pathway. These findings support the idea that MBG-NH /EGF is a potential biomaterial for bone tissue repair in bone defect-related diseases.
[Mh] Termos MeSH primário: Materiais Biocompatíveis/farmacologia
Regeneração Óssea/efeitos dos fármacos
Fator de Crescimento Epidérmico/farmacologia
Vidro/química
Osteoblastos/citologia
Engenharia Tecidual/métodos
Tecidos Suporte/química
[Mh] Termos MeSH secundário: Animais
Calcificação Fisiológica/efeitos dos fármacos
Adesão Celular/efeitos dos fármacos
Diferenciação Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Células Cultivadas
Subunidade alfa 1 de Fator de Ligação ao Core/metabolismo
Regulação da Expressão Gênica/efeitos dos fármacos
Sistema de Sinalização das MAP Quinases/efeitos dos fármacos
Camundongos
Osteoblastos/efeitos dos fármacos
Osteoblastos/metabolismo
Porosidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biocompatible Materials); 0 (Core Binding Factor Alpha 1 Subunit); 62229-50-9 (Epidermal Growth Factor)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1088/1748-605X/aa65d8


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[PMID]:29253565
[Au] Autor:Lee W; Ko KR; Kim HK; Lim S; Kim S
[Ad] Endereço:Department of Biological Sciences, Seoul National University, Seoul 151-742, South Korea; ViroMed Co., Ltd., Seoul 151-747, South Korea.
[Ti] Título:Dehydrodiconiferyl alcohol promotes BMP-2-induced osteoblastogenesis through its agonistic effects on estrogen receptor.
[So] Source:Biochem Biophys Res Commun;495(3):2242-2248, 2018 01 15.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Estrogen deficiency results in an imbalance between the levels of bone-resorping osteoclasts and bone-forming osteoblasts, eventually leading to overall bone loss. Dehydrodiconiferyl alcohol (DHCA), a lignan compound originally isolated from Cucurbita moschata, has been shown to bind to estrogen receptor, and indeed exhibits various activities of estrogen, such as anti-inflammatory and anti-oxidative stress effects. In this study, we tested whether synthetic DHCA could affect the BMP-2-induced osteoblastogenesis in vitro. In MC3T3-E1 cells, DHCA promoted BMP-2-induced differentiation of osteoblasts. Consistently, the expression of three osteoblastogenic genes known to be induced by BMP-2, ALP, osteocalcin and OPG, was up-regulated by DHCA treatment. DHCA was also shown to activate the production of RUNX2 by activating Smad1/5/9 and AMPK. Data from transient transfection assays suggested that DHCA might activate the estrogen receptor signaling pathway. Effects of DHCA on BMP-2-induced osteoblastogenesis were reduced when cells were treated with a specific siRNA to ERα or ERß. Taken together, our results suggest that DHCA may be developed as an efficient therapeutic for osteoporosis by regulating osteoblastogenesis through its estrogenic effects.
[Mh] Termos MeSH primário: Proteína Morfogenética Óssea 2/metabolismo
Moduladores de Receptor Estrogênico/administração & dosagem
Estrogênios/metabolismo
Osteoblastos/fisiologia
Osteogênese/fisiologia
Fenóis/administração & dosagem
Receptores Estrogênicos/metabolismo
[Mh] Termos MeSH secundário: Células 3T3
Animais
Diferenciação Celular/efeitos dos fármacos
Diferenciação Celular/fisiologia
Relação Dose-Resposta a Droga
Camundongos
Osteoblastos/citologia
Osteoblastos/efeitos dos fármacos
Osteogênese/efeitos dos fármacos
Receptores Estrogênicos/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bmp2 protein, mouse); 0 (Bone Morphogenetic Protein 2); 0 (Estrogen Receptor Modulators); 0 (Estrogens); 0 (Phenols); 0 (Receptors, Estrogen); 4263-87-0 (dehydrodiconiferyl alcohol)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE



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