Base de dados : MEDLINE
Pesquisa : A11.497.124 [Categoria DeCS]
Referências encontradas : 33 [refinar]
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[PMID]:28457750
[Au] Autor:Li L; Dong J; Yan L; Yong J; Liu X; Hu Y; Fan X; Wu X; Guo H; Wang X; Zhu X; Li R; Yan J; Wei Y; Zhao Y; Wang W; Ren Y; Yuan P; Yan Z; Hu B; Guo F; Wen L; Tang F; Qiao J
[Ad] Endereço:Beijing Advanced Innovation Center for Genomics (ICG), College of Life Sciences, Department of Obstetrics and Gynecology, Third Hospital, Peking University, Beijing 100871, China; Biomedical Institute for Pioneering Investigation via Convergence and Center for Reproductive Medicine, Ministry of Educ
[Ti] Título:Single-Cell RNA-Seq Analysis Maps Development of Human Germline Cells and Gonadal Niche Interactions.
[So] Source:Cell Stem Cell;20(6):858-873.e4, 2017 Jun 01.
[Is] ISSN:1875-9777
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Human fetal germ cells (FGCs) are precursors to sperm and eggs and are crucial for maintenance of the species. However, the developmental trajectories and heterogeneity of human FGCs remain largely unknown. Here we performed single-cell RNA-seq analysis of over 2,000 FGCs and their gonadal niche cells in female and male human embryos spanning several developmental stages. We found that female FGCs undergo four distinct sequential phases characterized by mitosis, retinoic acid signaling, meiotic prophase, and oogenesis. Male FGCs develop through stages of migration, mitosis, and cell-cycle arrest. Individual embryos of both sexes simultaneously contain several subpopulations, highlighting the asynchronous and heterogeneous nature of FGC development. Moreover, we observed reciprocal signaling interactions between FGCs and their gonadal niche cells, including activation of the bone morphogenic protein (BMP) and Notch signaling pathways. Our work provides key insights into the crucial features of human FGCs during their highly ordered mitotic, meiotic, and gametogenetic processes in vivo.
[Mh] Termos MeSH primário: Divisão Celular/fisiologia
Células Germinativas Embrionárias/metabolismo
Feto/metabolismo
Gônadas/enzimologia
Transdução de Sinais/fisiologia
Nicho de Células-Tronco/fisiologia
[Mh] Termos MeSH secundário: Proteínas Morfogenéticas Ósseas/metabolismo
Células Germinativas Embrionárias/citologia
Feminino
Feto/citologia
Gônadas/citologia
Sequenciamento de Nucleotídeos em Larga Escala
Seres Humanos
Masculino
Receptores Notch/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bone Morphogenetic Proteins); 0 (Receptors, Notch)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


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[PMID]:28687666
[Au] Autor:LeBlanc MG; Lehmann R
[Ad] Endereço:Howard Hughes Medical Institute, Kimmel Center for Biology and Medicine of the Skirball Institute, New York University School of Medicine, New York, NY.
[Ti] Título:Domain-specific control of germ cell polarity and migration by multifunction Tre1 GPCR.
[So] Source:J Cell Biol;216(9):2945-2958, 2017 Sep 04.
[Is] ISSN:1540-8140
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The migration of primordial germ cells (PGCs) from their place of origin to the embryonic gonad is an essential reproductive feature in many animal species. In , a single G protein-coupled receptor, Trapped in endoderm 1 (Tre1), mediates germ cell polarization at the onset of active migration and directs subsequent migration of PGCs through the midgut primordium. How these different aspects of cell behavior are coordinated through a single receptor is not known. We demonstrate that two highly conserved domains, the E/N/DRY and NPxxY motifs, have overlapping and unique functions in Tre1. The Tre1-NRY domain via G protein signaling is required for reading and responding to guidance and survival cues controlled by the lipid phosphate phosphatases Wunen and Wunen2. In contrast, the Tre1-NPIIY domain has a separate role in Rho1- and E-cadherin-mediated polarization at the initiation stage independent of G protein signaling. We propose that this bifurcation of the Tre1 G protein-coupled receptor signaling response via G protein-dependent and independent branches enables distinct spatiotemporal regulation of germ cell migration.
[Mh] Termos MeSH primário: Polaridade Celular
Proteínas de Drosophila/metabolismo
Drosophila melanogaster/metabolismo
Células Germinativas Embrionárias/metabolismo
Receptores Acoplados a Proteínas-G/metabolismo
[Mh] Termos MeSH secundário: Animais
Animais Geneticamente Modificados
Caderinas/genética
Caderinas/metabolismo
Movimento Celular
Proteínas de Drosophila/genética
Drosophila melanogaster/embriologia
Drosophila melanogaster/genética
Genótipo
Ligantes
Proteínas de Membrana/genética
Proteínas de Membrana/metabolismo
Microscopia de Fluorescência
Microscopia de Vídeo
Mutação
Fenótipo
Fosfatidato Fosfatase/genética
Fosfatidato Fosfatase/metabolismo
Domínios Proteicos
Receptores Acoplados a Proteínas-G/genética
Transdução de Sinais
Proteínas rho de Ligação ao GTP/genética
Proteínas rho de Ligação ao GTP/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; VIDEO-AUDIO MEDIA
[Nm] Nome de substância:
0 (Cadherins); 0 (Drosophila Proteins); 0 (Ligands); 0 (Membrane Proteins); 0 (Receptors, G-Protein-Coupled); 0 (Tre1 protein, Drosophila); 0 (shotgun protein, Drosophila); EC 3.1.3.4 (Phosphatidate Phosphatase); EC 3.1.3.4 (Wun2 protein, Drosophila); EC 3.1.3.4 (wunen protein, Drosophila); EC 3.6.5.2 (Rho1 protein, Drosophila); EC 3.6.5.2 (rho GTP-Binding Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170709
[St] Status:MEDLINE
[do] DOI:10.1083/jcb.201612053


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[PMID]:28215675
[Au] Autor:Wang C; Deng Y; Chen F; Zhu P; Wei J; Luo C; Lu F; Yang S; Shi D
[Ad] Endereço:Animal Reproduction Institute, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning, China; Reproductive Medicine Center, Maternal and Child Health Hospital of Guangxi Zhuang Autonomous Region, Nanning, China.
[Ti] Título:Basic fibroblast growth factor is critical to reprogramming buffalo (Bubalus bubalis) primordial germ cells into embryonic germ stem cell-like cells.
[So] Source:Theriogenology;91:112-120, 2017 Mar 15.
[Is] ISSN:1879-3231
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Primordial germ cells (PGCs) are destined to form gametes in vivo, and they can be reprogrammed into pluripotent embryonic germ (EG) cells in vitro. Buffalo PGC have been reported to be reprogrammed into EG-like cells, but the identities of the major signaling pathways and culture media involved in this derivation remain unclear. Here, the effects of basic fibroblast growth factor (bFGF) and downstream signaling pathways on the reprogramming of buffalo PGCs into EG-like cells were investigated. Results showed bFGF to be critical to buffalo PGCs to dedifferentiate into EG-like cells (20 ng/mL is optimal) with many characteristics of pluripotent stem cells, including alkaline phosphatase (AP) activity, expression of pluripotency marker genes such as OCT4, NANOG, SOX2, SSEA-1, CDH1, and TRA-1-81, and the capacity to differentiate into all three embryonic germ layers. After chemically inhibiting pathways or components downstream of bFGF, data showed that inhibition of the PI3K/AKT pathway led to significantly lower EG cell derivation, while inhibition of P53 activity resulted in an efficiency of EG cell derivation comparable to that in the presence of bFGF. These results suggest that the role of bFGF in PGC-derived EG-like cell generation is mainly due to the activation of the PI3K/AKT/P53 pathway, in particular, the inhibition of P53 function.
[Mh] Termos MeSH primário: Búfalos/embriologia
Células Germinativas Embrionárias/efeitos dos fármacos
Fatores de Crescimento de Fibroblastos/farmacologia
[Mh] Termos MeSH secundário: Animais
Búfalos/crescimento & desenvolvimento
Búfalos/metabolismo
Técnicas de Cultura de Células/veterinária
Diferenciação Celular/efeitos dos fármacos
Linhagem da Célula
Reprogramação Celular
Células Germinativas Embrionárias/metabolismo
Células Germinativas Embrionárias/fisiologia
Sistema de Sinalização das MAP Quinases
Células-Tronco Pluripotentes
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
62031-54-3 (Fibroblast Growth Factors)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170221
[St] Status:MEDLINE


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[PMID]:28096217
[Au] Autor:Owens DA; Butler AM; Aguero TH; Newman KM; Van Booven D; King ML
[Ad] Endereço:Department of Cell Biology, University of Miami Miller School of Medicine, 1011 NW 15th St, Miami, FL 33136, USA.
[Ti] Título:High-throughput analysis reveals novel maternal germline RNAs crucial for primordial germ cell preservation and proper migration.
[So] Source:Development;144(2):292-304, 2017 01 15.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:During oogenesis, hundreds of maternal RNAs are selectively localized to the animal or vegetal pole, including determinants of somatic and germline fates. Although microarray analysis has identified localized determinants, it is not comprehensive and is limited to known transcripts. Here, we utilized high-throughput RNA-sequencing analysis to comprehensively interrogate animal and vegetal pole RNAs in the fully grown Xenopus laevis oocyte. We identified 411 (198 annotated) and 27 (15 annotated) enriched mRNAs at the vegetal and animal pole, respectively. Ninety were novel mRNAs over 4-fold enriched at the vegetal pole and six were over 10-fold enriched at the animal pole. Unlike mRNAs, microRNAs were not asymmetrically distributed. Whole-mount in situ hybridization confirmed that all 17 selected mRNAs were localized. Biological function and network analysis of vegetally enriched transcripts identified protein-modifying enzymes, receptors, ligands, RNA-binding proteins, transcription factors and co-factors with five defining hubs linking 47 genes in a network. Initial functional studies of maternal vegetally localized mRNAs show that sox7 plays a novel and important role in primordial germ cell (PGC) development and that ephrinB1 (efnb1) is required for proper PGC migration. We propose potential pathways operating at the vegetal pole that highlight where future investigations might be most fruitful.
[Mh] Termos MeSH primário: Movimento Celular/genética
Células Germinativas Embrionárias/fisiologia
Células Germinativas/metabolismo
RNA Mensageiro Estocado/genética
RNA/metabolismo
Xenopus laevis
[Mh] Termos MeSH secundário: Animais
Animais Geneticamente Modificados
Células Germinativas Embrionárias/metabolismo
Feminino
Sequenciamento de Nucleotídeos em Larga Escala
Oócitos/metabolismo
Oogênese/genética
RNA/análise
RNA/genética
RNA Mensageiro Estocado/metabolismo
Xenopus laevis/embriologia
Xenopus laevis/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (RNA, Messenger, Stored); 63231-63-0 (RNA)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171125
[Lr] Data última revisão:
171125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170119
[St] Status:MEDLINE
[do] DOI:10.1242/dev.139220


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[PMID]:27876644
[Au] Autor:de Oliveira PR; Anholeto LA; Bechara GH; Camargo Mathias MI
[Ad] Endereço:Department of Biology, Institute of Biosciences, São Paulo State University-UNESP, Av. 24A, n° 1515, Postal Code 199, Zip Code: 13506-900, Rio Claro, SP, Brazil. Electronic address: patyrosaoliv@yahoo.com.br.
[Ti] Título:Dinotefuran-induced morphophysiological changes in semi-engorged females Rhipicephalus sanguineus Latreille, 1806 (Acari: Ixodidae) ticks: Ultra-structural evaluation.
[So] Source:Acta Trop;166:139-154, 2017 Feb.
[Is] ISSN:1873-6254
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The present study demonstrated the effects of dinotefuran (active ingredient of the acaricide Protetor Pet ) on the ovary and midgut cells of semi engorged R. sanguineus females exposed to different concentrations of this chemical. For this, 120 semi-engorged females were divided into four treatment groups with 30 individuals each: group I or control (distilled water), group II (5000ppm), groups III (6250ppm) and group IV (8334ppm of dinotefuran). All the ticks were immersed in the different concentrations of dinotefuran or in distilled water for 5min and then dried and kept in BOD incubator for 7days. The results showed alterations mainly regarding the damaged cell structures, such as yolk granules, organelles and the plasma membrane of the germ cells. In addition, structures related with defense mechanisms were found, such as vacuoles, cytoskeletal filaments, and myelin figures in the germ cells. Damages in the generative cells of the midgut, alterations in the size of digestive cells, the number of endosomes, digestive vacuoles, digestive residues, lipid drops and organelles in the cytoplasm of the digestive cells and the presence of microvilli in the plasma membrane of these cells also demonstrate the progressive damages caused by the action of dinotefuran in the midgut and germ cells of R. sanguineus semi-engorged females. The concentrations applied partially impaired the digestive processes; and, without proper nutrition, all the ectoparasite's physiologic events are prevented from occurring, leading the individual to death. The germ cells were also damaged, and probably would not be able to advance in their development (I-V) and complete the vitellogenesis, which would affect the fertility of the female and consequently impede the formation of a new individual.
[Mh] Termos MeSH primário: Acaricidas/farmacologia
Sistema Digestório/efeitos dos fármacos
Guanidinas/farmacologia
Nitrocompostos/farmacologia
Ovário/efeitos dos fármacos
Rhipicephalus sanguineus/ultraestrutura
[Mh] Termos MeSH secundário: Animais
Citoplasma/efeitos dos fármacos
Citoplasma/ultraestrutura
Sistema Digestório/ultraestrutura
Gema de Ovo/efeitos dos fármacos
Gema de Ovo/ultraestrutura
Células Germinativas Embrionárias/efeitos dos fármacos
Células Germinativas Embrionárias/ultraestrutura
Feminino
Fertilidade/efeitos dos fármacos
Neonicotinoides
Organelas/efeitos dos fármacos
Organelas/ultraestrutura
Ovário/ultraestrutura
Rhipicephalus sanguineus/efeitos dos fármacos
Vitelogênese/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acaricides); 0 (Guanidines); 0 (Neonicotinoids); 0 (Nitro Compounds); 1W509710WF (dinotefuran)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161124
[St] Status:MEDLINE


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[PMID]:27923120
[Au] Autor:Hurd TR; Herrmann B; Sauerwald J; Sanny J; Grosch M; Lehmann R
[Ad] Endereço:Department of Cell Biology, HHMI and Kimmel Center for Biology and Medicine of the Skirball Institute, New York University School of Medicine, New York, NY 10016, USA.
[Ti] Título:Long Oskar Controls Mitochondrial Inheritance in Drosophila melanogaster.
[So] Source:Dev Cell;39(5):560-571, 2016 Dec 05.
[Is] ISSN:1878-1551
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Inherited mtDNA mutations cause severe human disease. In most species, mitochondria are inherited maternally through mechanisms that are poorly understood. Genes that specifically control the inheritance of mitochondria in the germline are unknown. Here, we show that the long isoform of the protein Oskar regulates the maternal inheritance of mitochondria in Drosophila melanogaster. We show that, during oogenesis, mitochondria accumulate at the oocyte posterior, concurrent with the bulk streaming and churning of the oocyte cytoplasm. Long Oskar traps and maintains mitochondria at the posterior at the site of primordial germ cell (PGC) formation through an actin-dependent mechanism. Mutating long oskar strongly reduces the number of mtDNA molecules inherited by PGCs. Therefore, Long Oskar ensures germline transmission of mitochondria to the next generation. These results provide molecular insight into how mitochondria are passed from mother to offspring, as well as how they are positioned and asymmetrically partitioned within polarized cells.
[Mh] Termos MeSH primário: Proteínas de Drosophila/genética
Drosophila melanogaster/genética
Genes de Insetos
Genes Mitocondriais/genética
[Mh] Termos MeSH secundário: Actinas/metabolismo
Animais
Variações do Número de Cópias de DNA
DNA Mitocondrial/genética
DNA Mitocondrial/metabolismo
Drosophila melanogaster/embriologia
Drosophila melanogaster/metabolismo
Células Germinativas Embrionárias/metabolismo
Feminino
Seres Humanos
Oogênese/genética
Isoformas de Proteínas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Actins); 0 (DNA, Mitochondrial); 0 (Drosophila Proteins); 0 (Protein Isoforms); 0 (oskar protein, Drosophila)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170613
[Lr] Data última revisão:
170613
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161207
[St] Status:MEDLINE


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[PMID]:27705743
[Au] Autor:Lee C; Sorensen EB; Lynch TR; Kimble J
[Ad] Endereço:Howard Hughes Medical Institute, University of Wisconsin-Madison, Madison, United States.
[Ti] Título: GLP-1/Notch activates transcription in a probability gradient across the germline stem cell pool.
[So] Source:Elife;5, 2016 Oct 05.
[Is] ISSN:2050-084X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Notch signaling maintains a pool of germline stem cells within their single-celled mesenchymal niche. Here we investigate the Notch transcriptional response in germline stem cells using single-molecule fluorescence hybridization coupled with automated, high-throughput quantitation. This approach allows us to distinguish Notch-dependent nascent transcripts in the nucleus from mature mRNAs in the cytoplasm. We find that Notch-dependent active transcription sites occur in a probabilistic fashion and, unexpectedly, do so in a steep gradient across the stem cell pool. Yet these graded nuclear sites create a nearly uniform field of mRNAs that extends beyond the region of transcriptional activation. Therefore, active transcription sites provide a precise view of where the Notch-dependent transcriptional complex is productively engaged. Our findings offer a new window into the Notch transcriptional response and demonstrate the importance of assaying nascent transcripts at active transcription sites as a readout for canonical signaling.
[Mh] Termos MeSH primário: Proteínas de Caenorhabditis elegans/metabolismo
Caenorhabditis elegans/fisiologia
Células Germinativas Embrionárias/fisiologia
Receptores Notch/metabolismo
Células-Tronco/fisiologia
Transcrição Genética
[Mh] Termos MeSH secundário: Animais
Caenorhabditis elegans/embriologia
Hibridização in Situ Fluorescente
Imagem Individual de Molécula
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Caenorhabditis elegans Proteins); 0 (Glp-1 protein, C elegans); 0 (Receptors, Notch)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161006
[St] Status:MEDLINE


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[PMID]:27618755
[Au] Autor:Hanlon CD; Andrew DJ
[Ad] Endereço:Department of Cell Biology, The Johns Hopkins University School of Medicine, 725 N. Wolfe St., Baltimore, MD 21205-2196, United States.
[Ti] Título:Drosophila FoxL1 non-autonomously coordinates organ placement during embryonic development.
[So] Source:Dev Biol;419(2):273-284, 2016 11 15.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Determining how organs attain precise positioning within an organism is a crucial facet of developmental biology. The Fox family winged-helix transcription factors are known to play key roles in development of multiple organs. Drosophila FoxL1 (aka Fd64A) is dynamically expressed in embryos but its function is completely uncharacterized. FoxL1 is expressed in a single group of body wall - muscles in the 2nd and 3rd thoracic segments, in homologous abdominal muscles at earlier stages, and in the hindgut mesoderm from early through late embryogenesis. We show that FoxL1 expression in T2 and T3 is in VIS5, which is not a single muscle spanning the entire thorax, as previously published, but is, instead, three individual muscles, each spanning a single thoracic segment. We generate mutations in foxL1 and show that, surprisingly, none of the tissues that express FoxL1 are affected by its loss. Instead, loss of foxL1 results in defects in salivary gland positioning and morphology, as well as defects in the migration of hemocytes, germ cells and Malpighian tubules. We also show that FoxL1-dependent expression of secreted Sema2a in T3 VIS5 is required for normal salivary gland positioning. Altogether, these findings suggest that Drosophila FoxL1 functions like its mammalian counterpart in non-autonomously orchestrating the behaviors of surrounding tissues.
[Mh] Termos MeSH primário: Proteínas de Drosophila/fisiologia
Drosophila melanogaster/embriologia
Fatores de Transcrição Forkhead/fisiologia
Organogênese/fisiologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Padronização Corporal/genética
Padronização Corporal/fisiologia
Movimento Celular/genética
Movimento Celular/fisiologia
Proteínas de Drosophila/deficiência
Proteínas de Drosophila/genética
Drosophila melanogaster/genética
Embrião não Mamífero/ultraestrutura
Células Germinativas Embrionárias/citologia
Fatores de Transcrição Forkhead/deficiência
Fatores de Transcrição Forkhead/genética
Regulação da Expressão Gênica no Desenvolvimento
Hemócitos/citologia
Túbulos de Malpighi/embriologia
Músculos/embriologia
Músculos/ultraestrutura
Especificidade de Órgãos
Organogênese/genética
Interferência de RNA
Proteínas Recombinantes de Fusão/genética
Glândulas Salivares/embriologia
Alinhamento de Sequência
Homologia de Sequência de Aminoácidos
Tórax/embriologia
Tórax/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Drosophila Proteins); 0 (Forkhead Transcription Factors); 0 (FoxL1 homolog protein, Drosophila); 0 (Recombinant Fusion Proteins)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171115
[Lr] Data última revisão:
171115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160914
[St] Status:MEDLINE


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[PMID]:27435625
[Au] Autor:Sun J; Ting MC; Ishii M; Maxson R
[Ad] Endereço:Department of Biochemistry and Molecular Biology, Norris Cancer Hospital, University of Southern California, Keck School of Medicine, 1441 Eastlake Avenue, Los Angeles, CA 90089-9176, USA.
[Ti] Título:Msx1 and Msx2 function together in the regulation of primordial germ cell migration in the mouse.
[So] Source:Dev Biol;417(1):11-24, 2016 09 01.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Primordial germ cells (PGCs) are a highly migratory cell population that gives rise to eggs and sperm. Much is known about PGC specification, but less about the processes that control PGC migration. In this study, we document a deficiency in PGC development in embryos carrying global homozygous null mutations in Msx1 and Msx2, both immediate downstream effectors of Bmp signaling pathway. We show that Msx1(-/-);Msx2(-/-) mutant embryos have defects in PGC migration as well as a reduced number of PGCs. These phenotypes are also evident in a Mesp1-Cre-mediated mesoderm-specific mutant line of Msx1 and Msx2. Since PGCs are not marked in Mesp1-lineage tracing, our results suggest that Msx1 and Msx2 function cell non-autonomously in directing PGC migration. Consistent with this hypothesis, we noted an upregulation of fibronectin, well known as a mediator of cell migration, in tissues through which PGCs migrate. We also noted a reduction in the expression of Wnt5a and an increase in the expression in Bmp4 in such tissues in Msx1(-/-);Msx2(-/-) mutants, both known effectors of PGC development.
[Mh] Termos MeSH primário: Movimento Celular/genética
Células Germinativas Embrionárias/citologia
Proteínas de Homeodomínio/genética
Fator de Transcrição MSX1/genética
[Mh] Termos MeSH secundário: Animais
Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo
Proteína Morfogenética Óssea 4/biossíntese
Células Germinativas Embrionárias/metabolismo
Fibronectinas/biossíntese
Proteínas de Homeodomínio/metabolismo
Fator de Transcrição MSX1/metabolismo
Mesoderma/citologia
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Knockout
Proteína Wnt-5a/biossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Basic Helix-Loop-Helix Transcription Factors); 0 (Bmp4 protein, mouse); 0 (Bone Morphogenetic Protein 4); 0 (Fibronectins); 0 (Homeodomain Proteins); 0 (MSX1 Transcription Factor); 0 (MSX2 protein); 0 (Mesp1 protein, mouse); 0 (Msx1 protein, mouse); 0 (Wnt-5a Protein); 0 (Wnt5a protein, mouse)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160721
[St] Status:MEDLINE


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[PMID]:27411809
[Au] Autor:Iqbal K; Tran DA; Li AX; Warden C; Bai AY; Singh P; Madaj ZB; Winn ME; Wu X; Pfeifer GP; Szabó PE
[Ad] Endereço:Beckman Research Institute Irell and Manella Graduate School of Biological Sciences, Duarte, CA, USA.
[Ti] Título:High type I error and misrepresentations in search for transgenerational epigenetic inheritance: response to Guerrero-Bosagna.
[So] Source:Genome Biol;17(1):154, 2016 Jul 12.
[Is] ISSN:1474-760X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In a recent paper, we described our efforts in search for evidence supporting epigenetic transgenerational inheritance caused by endocrine disrupter chemicals. One aspect of our study was to compare genome-wide DNA methylation changes in the vinclozolin-exposed fetal male germ cells (n = 3) to control samples (n = 3), their counterparts in the next, unexposed, generation (n = 3 + 3) and also in adult spermatozoa (n = 2 + 2) in both generations. We reported finding zero common hits in the intersection of these four comparisons. In our interpretation, this result did not support the notion that DNA methylation provides a mechanism for a vinclozolin-induced transgenerational male infertility phenotype. In response to criticism by Guerrero-Bosagna regarding our statistical power in the above study, here we provide power calculations to clarify the statistical power of our study and to show the validity of our conclusions. We also explain here how our data is misinterpreted in the commentary by Guerrero-Bosagna by leaving out important data points from consideration.Please see related Correspondence article: xxx (13059_2016_982) and related Research article: http://genomebiology.biomedcentral.com/articles/10.1186/s13059-015-0619-z.
[Mh] Termos MeSH primário: Metilação de DNA/genética
Disruptores Endócrinos/toxicidade
Epigênese Genética
Oxazóis/toxicidade
[Mh] Termos MeSH secundário: Metilação de DNA/efeitos dos fármacos
Células Germinativas Embrionárias/efeitos dos fármacos
Células Germinativas Embrionárias/metabolismo
Seres Humanos
Masculino
Espermatozoides/efeitos dos fármacos
Espermatozoides/patologia
[Pt] Tipo de publicação:LETTER
[Nm] Nome de substância:
0 (Endocrine Disruptors); 0 (Oxazoles); JJ258EZN1I (vinclozolin)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170614
[Lr] Data última revisão:
170614
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160715
[St] Status:MEDLINE
[do] DOI:10.1186/s13059-016-0981-5



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