Base de dados : MEDLINE
Pesquisa : A11.600 [Categoria DeCS]
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[PMID]:27770776
[Au] Autor:Jooste M; Dreyer LL; Oberlander KC
[Ad] Endereço:Department of Botany and Zoology, University of Stellenbosch, Private Bag X1, Matieland, 7602, South Africa. mich.jooste.m@gmail.com.
[Ti] Título:The phylogenetic significance of leaf anatomical traits of southern African Oxalis.
[So] Source:BMC Evol Biol;16(1):225, 2016 10 22.
[Is] ISSN:1471-2148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The southern African Oxalis radiation is extremely morphologically variable. Despite recent progress in the phylogenetics of the genus, there are few morphological synapomorphies supporting DNA-based clades. Leaflet anatomy can provide an understudied and potentially valuable source of information on the evolutionary history and systematics of this lineage. Fifty-nine leaflet anatomical traits of 109 southern African Oxalis species were assessed in search of phylogenetically significant characters that delineate clades. RESULTS: A combination of 6 leaflet anatomical traits (stomatal position, adaxial epidermal cells, abaxial epidermal cells, mesophyll, sheath around vascular tissue, degree of leaflet conduplication) clearly support various clades defined by previous DNA-based phylogenetic work. Other, mostly continuous leaflet anatomical traits were highly variable and showed less phylogenetic pattern. CONCLUSIONS: Major and unexpected findings include the transition from ancestral hypostomatic leaflets to adaxially-located stomata in the vast majority of southern African Oxalis, the loss of semi-swollen AB epidermal cells and the gain of swollen adaxial and abaxial epidermal cells in selected clades, and multiple changes from ancestral bifacial mesophyll to isobilateral or homogenous mesophyll types. The information gathered in this study will aid in the taxonomic revision of this speciose member of the Greater Cape Floristic Region and provide a basis for future hypotheses regarding its radiation.
[Mh] Termos MeSH primário: Oxalidaceae/anatomia & histologia
Filogenia
Folhas de Planta/anatomia & histologia
[Mh] Termos MeSH secundário: Evolução Biológica
Duplicação Gênica
Células do Mesofilo/citologia
Oxalidaceae/genética
Fenótipo
Folhas de Planta/citologia
Folhas de Planta/genética
Estômatos de Plantas/citologia
Feixe Vascular de Plantas/citologia
Característica Quantitativa Herdável
Tricomas/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1708
[Cu] Atualização por classe:180128
[Lr] Data última revisão:
180128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


  2 / 414 MEDLINE  
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[PMID]:29175728
[Au] Autor:Zhou Y; Zhang B; Stuart-Williams H; Grice K; Hocart CH; Gessler A; Kayler ZE; Farquhar GD
[Ad] Endereço:School of Chemistry & Chemical Engineering, Shaanxi University of Science & Technology, Xi'an, China; Institute for Landscape Biogeochemistry, ZALF, Germany; Leibniz Institute for Freshwater Ecology & Inland Fisheries, Germany. Electronic address: youping.zhou@sust.edu.cn.
[Ti] Título:On the contributions of photorespiration and compartmentation to the contrasting intramolecular H profiles of C and C plant sugars.
[So] Source:Phytochemistry;145:197-206, 2018 Jan.
[Is] ISSN:1873-3700
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Compartmentation of C photosynthetic biochemistry into bundle sheath (BS) and mesophyll (M) cells, and photorespiration in C plants is predicted to have hydrogen isotopic consequences for metabolites at both molecular and site-specific levels. Molecular-level evidence was recently reported (Zhou et al., 2016), but evidence at the site-specific level is still lacking. We propose that such evidence exists in the contrasting H distribution profiles of glucose samples from naturally grown C , C and CAM plants: photorespiration contributes to the relative H enrichment in H and relative H depletion in H & H (the average of the two pro-chiral Hs and in particular H ) in C glucose, while H-enriched C mesophyll cellular (chloroplastic) water most likely contributes to the enrichment at H ; export of (transferable hydrogen atoms of) NADPH from C mesophyll cells to bundle sheath cells (via the malate shuttle) and incorporation of H-relatively unenriched BS cellular water contribute to the relative depletion of H & H respectively; shuttling of triose-phosphates (PGA: phosphoglycerate dand DHAP: dihydroacetone phosphate) between C bundle sheath and mesophyll cells contributes to the relative enrichment in H & H (in particular H ) in C glucose.
[Mh] Termos MeSH primário: Deutério/química
Glucose/química
Plantas/química
[Mh] Termos MeSH secundário: Configuração de Carboidratos
Deutério/metabolismo
Glucose/metabolismo
Células do Mesofilo/metabolismo
Plantas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
AR09D82C7G (Deuterium); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180105
[Lr] Data última revisão:
180105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE


  3 / 414 MEDLINE  
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[PMID]:29065171
[Au] Autor:Ju S; Wang L; Zhang C; Yin T; Shao S
[Ad] Endereço:School of Environment and Spatial Informatics, China University of Mining & Technology, Xuzhou, Jiangsu, China.
[Ti] Título:Alleviatory effects of silicon on the foliar micromorphology and anatomy of rice (Oryza sativa L.) seedlings under simulated acid rain.
[So] Source:PLoS One;12(10):e0187021, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Silicon (Si) is a macroelement in plants. The biological effects and mitigation mechanisms of silicon under environmental stress have become hot topics. The main objectives of this study were to elucidate the roles of Si in alleviating the effects on the phenotype, micromorphology and anatomy of the leaves of rice seedlings under acid rain stress. The results indicated that the combined or single effects of Si and simulated acid rain (SAR) stress on rice roots depended on the concentration of Si and the intensity of the SAR stress. The combined or single effects of the moderate concentration of Si (2.0 mM) and light SAR (pH 4.0) enhanced the growth of the rice leaves and the development of the mesophyll cells, and the combined effects were stronger than those of the single treatments. The high concentration of Si (4.0 mM) and severe SAR (pH 3.0 or 2.0) exerted deleterious effects. The incorporation of Si (2.0 or 4.0 mM) into SAR at pH values of 3.0 or 2.0 promoted rice leaf growth, decreased necrosis spots, maintained the structure and function of the mesophyll cells, increased the epicuticular wax content and wart-like protuberance (WP) density, and improved the stomatal characteristics of the leaves of rice seedlings more than the SAR only treatments. The alleviatory effects observed with a moderate concentration of Si (2.0 mM) were better than the effects obtained with the high concentration of Si (4.0 mM). The alleviatory effects were due to the enhancement of the mechanical barriers in the leaf epidermis.
[Mh] Termos MeSH primário: Chuva Ácida
Oryza/crescimento & desenvolvimento
Folhas de Planta/metabolismo
Silício/metabolismo
[Mh] Termos MeSH secundário: Células do Mesofilo/metabolismo
Oryza/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acid Rain); Z4152N8IUI (Silicon)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171025
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0187021


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[PMID]:28880924
[Au] Autor:Berghuijs HNC; Yin X; Ho QT; Retta MA; Verboven P; Nicolaï BM; Struik PC
[Ad] Endereço:Centre for Crop Systems Analysis, Wageningen University & Research, Droevendaalsesteeg 1, Wageningen, The Netherlands.
[Ti] Título:Localization of (photo)respiration and CO2 re-assimilation in tomato leaves investigated with a reaction-diffusion model.
[So] Source:PLoS One;12(9):e0183746, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The rate of photosynthesis depends on the CO2 partial pressure near Rubisco, Cc, which is commonly calculated by models using the overall mesophyll resistance. Such models do not explain the difference between the CO2 level in the intercellular air space and Cc mechanistically. This problem can be overcome by reaction-diffusion models for CO2 transport, production and fixation in leaves. However, most reaction-diffusion models are complex and unattractive for procedures that require a large number of runs, like parameter optimisation. This study provides a simpler reaction-diffusion model. It is parameterized by both leaf physiological and leaf anatomical data. The anatomical data consisted of the thickness of the cell wall, cytosol and stroma, and the area ratios of mesophyll exposed to the intercellular air space to leaf surfaces and exposed chloroplast to exposed mesophyll surfaces. The model was used directly to estimate photosynthetic parameters from a subset of the measured light and CO2 response curves; the remaining data were used for validation. The model predicted light and CO2 response curves reasonably well for 15 days old tomato (cv. Admiro) leaves, if (photo)respiratory CO2 release was assumed to take place in the inner cytosol or in the gaps between the chloroplasts. The model was also used to calculate the fraction of CO2 produced by (photo)respiration that is re-assimilated in the stroma, and this fraction ranged from 56 to 76%. In future research, the model should be further validated to better understand how the re-assimilation of (photo)respired CO2 is affected by environmental conditions and physiological parameters.
[Mh] Termos MeSH primário: Dióxido de Carbono/metabolismo
Luz
Lycopersicon esculentum/metabolismo
Modelos Biológicos
Folhas de Planta/metabolismo
[Mh] Termos MeSH secundário: Transporte Biológico/efeitos da radiação
Respiração Celular/efeitos da radiação
Simulação por Computador
Citosol/metabolismo
Difusão
Lycopersicon esculentum/efeitos da radiação
Células do Mesofilo/metabolismo
Folhas de Planta/efeitos da radiação
Reprodutibilidade dos Testes
Vacúolos/metabolismo
Vacúolos/efeitos da radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
142M471B3J (Carbon Dioxide)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170908
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183746


  5 / 414 MEDLINE  
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[PMID]:28323890
[Au] Autor:Huo A; Chen Z; Wang P; Yang L; Wang G; Wang D; Liao S; Cheng T; Chen J; Shi J
[Ad] Endereço:Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.
[Ti] Título:Establishment of transient gene expression systems in protoplasts from Liriodendron hybrid mesophyll cells.
[So] Source:PLoS One;12(3):e0172475, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Liriodendron is a genus of the magnolia family comprised of two flowering tree species that produce hardwoods of great ecological and economic value. However, only a limited amount of genetic research has been performed on the Liriodendron genus partly because transient or stable transgenic trees have been difficult to produce. In general, transient expression systems are indispensable for rapid, high-throughput screening and systematic characterization of gene functions at a low cost; therefore, development of such a system for Liriodendron would provide a necessary step forward for research on Magnoliaceae and other woody trees. Herein, we describe an efficient and rapid protocol for preparing protoplasts from the leaf mesophyll tissue of a Liriodendron hybrid and an optimized system for polyethylene glycol-mediated transient transfection of the protoplasts. Because the leaves of the Liriodendron hybrid are waxy, we formulated an enzyme mix containing 1.5% (w/v) Cellulase R-10, 0.5% (w/v) Macerozyme R-10, and 0.1% (w/v) Pectolyase Y-23 to efficiently isolate protoplasts from the Liriodendron hybrid leaf mesophyll tissue in 3 h. We optimized Liriodendron protoplast transfection efficiency by including 20 µg plasmid DNA per 104 protoplasts, a transformation time of 20 min, and inclusion of 20% (w/v) polyethylene glycol 4000. After integrating the Liriodendron WOX1 gene into pJIT166-GFP to produce a WOX1-GFP fusion product and transfecting it into isolated protoplasts, LhWOX1-GFP was found to localize to the nucleus according to its green fluorescence.
[Mh] Termos MeSH primário: Expressão Gênica
Liriodendron
Células do Mesofilo
Protoplastos
Transfecção
[Mh] Termos MeSH secundário: Técnicas de Cultura de Células
Vetores Genéticos
Proteínas Luminescentes/genética
Proteínas Luminescentes/metabolismo
Células do Mesofilo/metabolismo
Folhas de Planta
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Protoplastos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Luminescent Proteins); 0 (Plant Proteins)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170823
[Lr] Data última revisão:
170823
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170322
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0172475


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[PMID]:28292294
[Au] Autor:Wu JZ; Liu Q; Geng XS; Li KM; Luo LJ; Liu JP
[Ad] Endereço:Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Agriculture, Hainan University, Haikou, Hainan Province, 570228, China.
[Ti] Título:Highly efficient mesophyll protoplast isolation and PEG-mediated transient gene expression for rapid and large-scale gene characterization in cassava (Manihot esculenta Crantz).
[So] Source:BMC Biotechnol;17(1):29, 2017 Mar 14.
[Is] ISSN:1472-6750
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Cassava (Manihot esculenta Crantz) is a major crop extensively cultivated in the tropics as both an important source of calories and a promising source for biofuel production. Although stable gene expression have been used for transgenic breeding and gene function study, a quick, easy and large-scale transformation platform has been in urgent need for gene functional characterization, especially after the cassava full genome was sequenced. METHODS: Fully expanded leaves from in vitro plantlets of Manihot esculenta were used to optimize the concentrations of cellulase R-10 and macerozyme R-10 for obtaining protoplasts with the highest yield and viability. Then, the optimum conditions (PEG4000 concentration and transfection time) were determined for cassava protoplast transient gene expression. In addition, the reliability of the established protocol was confirmed for subcellular protein localization. RESULTS: In this work we optimized the main influencing factors and developed an efficient mesophyll protoplast isolation and PEG-mediated transient gene expression in cassava. The suitable enzyme digestion system was established with the combination of 1.6% cellulase R-10 and 0.8% macerozyme R-10 for 16 h of digestion in the dark at 25 °C, resulting in the high yield (4.4 × 10 protoplasts/g FW) and vitality (92.6%) of mesophyll protoplasts. The maximum transfection efficiency (70.8%) was obtained with the incubation of the protoplasts/vector DNA mixture with 25% PEG4000 for 10 min. We validated the applicability of the system for studying the subcellular localization of MeSTP7 (an H /monosaccharide cotransporter) with our transient expression protocol and a heterologous Arabidopsis transient gene expression system. CONCLUSION: We optimized the main influencing factors and developed an efficient mesophyll protoplast isolation and transient gene expression in cassava, which will facilitate large-scale characterization of genes and pathways in cassava.
[Mh] Termos MeSH primário: Perfilação da Expressão Gênica/métodos
Melhoramento Genético/métodos
Manihot/genética
Proteínas de Plantas/genética
Engenharia de Proteínas/métodos
Protoplastos/citologia
[Mh] Termos MeSH secundário: Ensaios de Triagem em Larga Escala
Células do Mesofilo/citologia
Polietilenoglicóis
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); 30IQX730WE (Polyethylene Glycols)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170613
[Lr] Data última revisão:
170613
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170316
[St] Status:MEDLINE
[do] DOI:10.1186/s12896-017-0349-2


  7 / 414 MEDLINE  
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[PMID]:28248997
[Au] Autor:Jensen JK; Wilkerson CG
[Ad] Endereço:Department of Plant Biology, Michigan State University, East Lansing, MI, United States of America.
[Ti] Título:Brachypodium as an experimental system for the study of stem parenchyma biology in grasses.
[So] Source:PLoS One;12(3):e0173095, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Stem parenchyma is a major cell type that serves key metabolic functions for the plant especially in large grasses, such as sugarcane and sweet sorghum, where it serves to store sucrose or other products of photosynthesis. It is therefore desirable to understand the metabolism of this cell type as well as the mechanisms by which it provides its function for the rest of the plant. Ultimately, this information can be used to selectively manipulate this cell type in a controlled manner to achieve crop improvement. In this study, we show that Brachypodium distachyon is a useful model system for stem pith parenchyma biology. Brachypodium can be grown under condition where it resembles the growth patterns of important crops in that it produces large amounts of stem material with the lower leaves senescing and with significant stores of photosynthate located in the stem parenchyma cell types. We further characterize stem plastid morphology as a function of tissue types, as this organelle is central for a number of metabolic pathways, and quantify gene expression for the four main classes of starch biosynthetic genes. Notably, we find several of these genes differentially regulated between stem and leaf. These studies show, consistent with other grasses, that the stem functions as a specialized storage compartment in Brachypodium.
[Mh] Termos MeSH primário: Brachypodium/metabolismo
Células do Mesofilo/metabolismo
Melhoramento Vegetal/métodos
[Mh] Termos MeSH secundário: Brachypodium/genética
Brachypodium/crescimento & desenvolvimento
Regulação da Expressão Gênica no Desenvolvimento
Regulação da Expressão Gênica de Plantas
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Plastídeos/metabolismo
Amido/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); 9005-25-8 (Starch)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170302
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0173095


  8 / 414 MEDLINE  
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[PMID]:28197891
[Au] Autor:Yin X; Struik PC
[Ad] Endereço:Centre for Crop Systems Analysis, Wageningen University & Research, P.O. Box 430, 6700 AK, Wageningen, The Netherlands. Xinyou.yin@wur.nl.
[Ti] Título:Simple generalisation of a mesophyll resistance model for various intracellular arrangements of chloroplasts and mitochondria in C leaves.
[So] Source:Photosynth Res;132(2):211-220, 2017 May.
[Is] ISSN:1573-5079
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The classical definition of mesophyll conductance (g ) represents an apparent parameter (g ) as it places (photo)respired CO at the same compartment where the carboxylation by Rubisco takes place. Recently, Tholen and co-workers developed a framework, in which g better describes a physical diffusional parameter (g ). They partitioned mesophyll resistance (r = 1/g ) into two components, cell wall and plasmalemma resistance (r ) and chloroplast resistance (r ), and showed that g is sensitive to the ratio of photorespiratory (F) and respiratory (R ) CO release to net CO uptake (A): g = g /[1 + ω(F + R )/A], where ω is the fraction of r in r . We herein extend the framework further by considering various scenarios for the intracellular arrangement of chloroplasts and mitochondria. We show that the formula of Tholen et al. implies either that mitochondria, where (photo)respired CO is released, locate between the plasmalemma and the chloroplast continuum or that CO in the cytosol is completely mixed. However, the model of Tholen et al. is still valid if ω is replaced by ω(1-σ), where σ is the fraction of (photo)respired CO that experiences r (in addition to r and stomatal resistance) if this CO is to escape from being refixed. Therefore, responses of g to (F + R )/A lie somewhere between no sensitivity in the classical method (σ =1) and high sensitivity in the model of Tholen et al. (σ =0).
[Mh] Termos MeSH primário: Cloroplastos/metabolismo
Células do Mesofilo/metabolismo
Mitocôndrias/metabolismo
Modelos Biológicos
Folhas de Planta/metabolismo
[Mh] Termos MeSH secundário: Dióxido de Carbono/metabolismo
Fotossíntese/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
142M471B3J (Carbon Dioxide)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170627
[Lr] Data última revisão:
170627
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170216
[St] Status:MEDLINE
[do] DOI:10.1007/s11120-017-0340-8


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[PMID]:28153921
[Au] Autor:Buckley TN; John GP; Scoffoni C; Sack L
[Ad] Endereço:Plant Breeding Institute, Sydney Institute of Agriculture, University of Sydney, Narrabri 2390, Australia (T.N.B.); and t.buckley@sydney.edu.au.
[Ti] Título:The Sites of Evaporation within Leaves.
[So] Source:Plant Physiol;173(3):1763-1782, 2017 Mar.
[Is] ISSN:1532-2548
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The sites of evaporation within leaves are unknown, but they have drawn attention for decades due to their perceived implications for many factors, including patterns of leaf isotopic enrichment, the maintenance of mesophyll water status, stomatal regulation, and the interpretation of measured stomatal and leaf hydraulic conductances. We used a spatially explicit model of coupled water and heat transport outside the xylem, MOFLO 2.0, to map the distribution of net evaporation across leaf tissues in relation to anatomy and environmental parameters. Our results corroborate earlier predictions that most evaporation occurs from the epidermis at low light and moderate humidity but that the mesophyll contributes substantially when the leaf center is warmed by light absorption, and more so under high humidity. We also found that the bundle sheath provides a significant minority of evaporation (15% in darkness and 18% in high light), that the vertical center of amphistomatous leaves supports net condensation, and that vertical temperature gradients caused by light absorption vary over 10-fold across species, reaching 0.3°C. We show that several hypotheses that depend on the evaporating sites require revision in light of our findings, including that experimental measurements of stomatal and hydraulic conductances should be affected directly by changes in the location of the evaporating sites. We propose a new conceptual model that accounts for mixed-phase water transport outside the xylem. These conclusions have far-reaching implications for inferences in leaf hydraulics, gas exchange, water use, and isotope physiology.
[Mh] Termos MeSH primário: Algoritmos
Modelos Biológicos
Folhas de Planta/fisiologia
Transpiração Vegetal/fisiologia
Plantas/metabolismo
Água/metabolismo
[Mh] Termos MeSH secundário: Transporte Biológico/fisiologia
Transporte Biológico/efeitos da radiação
Simulação por Computador
Umidade
Luz
Células do Mesofilo/metabolismo
Células do Mesofilo/fisiologia
Epiderme Vegetal/citologia
Epiderme Vegetal/metabolismo
Epiderme Vegetal/fisiologia
Folhas de Planta/citologia
Folhas de Planta/metabolismo
Estômatos de Plantas/metabolismo
Transpiração Vegetal/efeitos da radiação
Plantas/classificação
Especificidade da Espécie
Temperatura Ambiente
Xilema/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
059QF0KO0R (Water)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170204
[St] Status:MEDLINE
[do] DOI:10.1104/pp.16.01605


  10 / 414 MEDLINE  
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[PMID]:28113076
[Au] Autor:Guo Q; Meng L; Humphreys MW; Scullion J; Mur LA
[Ad] Endereço:Beijing Research and Development Center for Grass and Environment, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, PR China.
[Ti] Título:Expression of FlHMA3, a P -ATPase from Festulolium loliaceum, correlates with response to cadmium stress.
[So] Source:Plant Physiol Biochem;112:270-277, 2017 Mar.
[Is] ISSN:1873-2690
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Heavy metal ATPase 3 (HMA3), a P -ATPase, is a key tonoplast transporter involved in mediating the vacuolar sequestration of cadmium (Cd) to detoxify the intake of this element by plants. HMA3 expression in response to Cd stress has not been previously examined in the grass hybrid species Festulolium loliaceum (Huds.) P. Fourn. In this study, FlHMA3 isolated from F. loliaceum was found to comprise 833 amino acid residues with 77% homology to the rice OsHMA3. Transient expression of FlHMA3 fused to enhanced green fluorescent protein in Arabidopsis protoplasts suggested its localization to vacuolar membranes. Quantitative real-time RT-PCR analysis of F. loliaceum revealed that FlHMA3 is expressed predominantly within roots and up-regulated by excess Cd. Over the 168 h treatment, Cd content of F. loliaceum roots was significantly higher than that of shoots, regardless of external CdCl concentrations. A significant positive correlation was found between FlHMA3 expression and Cd accumulation in roots of F. loliaceum seedlings subjected to 10-100 mg L CdCl for 168 h or, in a separate experiment, to 25 or 100 mg L CdCl for the same duration. These findings provide evidence that FlHMA3 encodes a vacuolar P -ATPase that may play an important role in Cd sequestration into root cell vacuoles, thereby limiting the entry of Cd into the cytoplasm and reducing Cd toxicity.
[Mh] Termos MeSH primário: Adenosina Trifosfatases/metabolismo
Cádmio/toxicidade
Proteínas de Plantas/metabolismo
Poaceae/enzimologia
Poaceae/fisiologia
Estresse Fisiológico/efeitos dos fármacos
[Mh] Termos MeSH secundário: Adenosina Trifosfatases/química
Adenosina Trifosfatases/genética
Sequência de Aminoácidos
Arabidopsis/genética
Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Células do Mesofilo/efeitos dos fármacos
Células do Mesofilo/metabolismo
Proteínas de Plantas/química
Proteínas de Plantas/genética
Plantas Geneticamente Modificadas
Poaceae/efeitos dos fármacos
Protoplastos/efeitos dos fármacos
Protoplastos/metabolismo
Alinhamento de Sequência
Estresse Fisiológico/genética
Frações Subcelulares/metabolismo
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); 00BH33GNGH (Cadmium); EC 3.6.1.- (Adenosine Triphosphatases)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170501
[Lr] Data última revisão:
170501
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170124
[St] Status:MEDLINE



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