Base de dados : MEDLINE
Pesquisa : A11.750 [Categoria DeCS]
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[PMID]:29262710
[Au] Autor:Liu Y; Liu G; Yang Y; Niu S; Yang F; Yang S; Tang J; Chen J
[Ad] Endereço:1 Department of Biotechnology, Faculty of Agricultural Science, Guang Dong Ocean University , Zhanjiang, Guangdong , P.R. China.
[Ti] Título:Establishment of an efficient plant regeneration culture protocol and achievement of successful genetic transformation in Jatropha curcas L.
[So] Source:Acta Biol Hung;68(4):428-442, 2017 Dec.
[Is] ISSN:0236-5383
[Cp] País de publicação:Hungary
[La] Idioma:eng
[Ab] Resumo:An efficient and reproducible protocol is described for shoot-bud regeneration and Agrobacterium tumefaciens-mediated genetic transformation of J. curcas. Treating the explants with high concentrations (5-120 mg/L) of TDZ for short durations (5-80 min) before inoculation culture increased significantly the regeneration frequency and improved the quality of the regenerated buds. The highest shoot-buds induction rate (87.35%) was achieved when petiole explants were treated with 20 mg/L TDZ solution for 20 min and inoculated on hormone-free MS medium for 30 days. Regenerated shoots of 0.5 cm or a little longer were isolated and grafted to seedling stocks of the same species, and then the grafted plantlets were planted on half-strength MS medium containing 0.1 mg/L IBA and 2 mg/L sodium nitroprusside (SNP). This grafting strategy was found to be very effective, to obtain that healthy grafted plantlets ready for acclimatization within 20 days. By the above mentioned protocol and with general Agrobacterium - mediated genetic transformation methods only 65 days were needed to obtain intact transgenic plants.
[Mh] Termos MeSH primário: Técnicas de Cultura de Células/métodos
Técnicas de Transferência de Genes
Jatropha
Células Vegetais/metabolismo
Brotos de Planta
Transformação Genética
[Mh] Termos MeSH secundário: Agrobacterium tumefaciens/genética
Agrobacterium tumefaciens/metabolismo
Jatropha/genética
Jatropha/crescimento & desenvolvimento
Brotos de Planta/genética
Brotos de Planta/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE
[do] DOI:10.1556/018.68.2017.4.8


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[PMID]:29307824
[Au] Autor:Yan J; He H; Fang L; Zhang A
[Ad] Endereço:National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China; College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.
[Ti] Título:Pectin methylesterase31 positively regulates salt stress tolerance in Arabidopsis.
[So] Source:Biochem Biophys Res Commun;496(2):497-501, 2018 02 05.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The alteration of cell wall component and structure is an important adaption to saline environment. Pectins, a major cell wall component, are often present in a highly methylesterified form. The level of methyl esterification determined by pectin methylesterases (PMEs) influences many important wall properties that are believed to relate to the adaption to saline stress. However, little is known about the function of PMEs in response to salt stress. Here, we established a link between pectin methylesterase31 (PME31) and salt stress tolerance. Salt stress significantly increases PME31 expression. PME31 is located in the plasma membrane and the expression level of PME31 was high in dry seeds. Knock-down mutants in PME31 conferred hypersensitive phenotypes to salt stress in seed germination and post-germination growth. Real-time PCR analysis revealed that the transcript levels of several stress genes (DREB2A, RD29A and RD29B) are lower in pme31-2 mutant than that in the wild type in response to salt stress. These results suggested that PME31 could positively modulate salt stress tolerance.
[Mh] Termos MeSH primário: Arabidopsis/efeitos dos fármacos
Hidrolases de Éster Carboxílico/genética
Regulação da Expressão Gênica de Plantas
Células Vegetais/efeitos dos fármacos
Cloreto de Sódio/farmacologia
[Mh] Termos MeSH secundário: Arabidopsis/genética
Arabidopsis/metabolismo
Proteínas de Arabidopsis/genética
Proteínas de Arabidopsis/metabolismo
Hidrolases de Éster Carboxílico/metabolismo
Membrana Celular/efeitos dos fármacos
Membrana Celular/metabolismo
Parede Celular/efeitos dos fármacos
Parede Celular/metabolismo
Proteínas e Peptídeos de Choque Frio/genética
Proteínas e Peptídeos de Choque Frio/metabolismo
Germinação/efeitos dos fármacos
Isoenzimas/genética
Isoenzimas/metabolismo
Mutação
Células Vegetais/metabolismo
Salinidade
Tolerância a Sal
Sementes/efeitos dos fármacos
Sementes/genética
Sementes/metabolismo
Estresse Fisiológico
Fatores de Transcrição/genética
Fatores de Transcrição/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Cold Shock Proteins and Peptides); 0 (DREB2A protein, Arabidopsis); 0 (Isoenzymes); 0 (RD29B protein, Arabidopsis); 0 (RD29a protein, Arabidopsis); 0 (Transcription Factors); 451W47IQ8X (Sodium Chloride); EC 3.1.1.- (Carboxylic Ester Hydrolases); EC 3.1.1.11 (pectinesterase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180109
[St] Status:MEDLINE


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[PMID]:29236392
[Au] Autor:Rudnytska MV; Palladina TA
[Ti] Título:Effect of preparations Methyure and Ivine on Са(2+)-ATPases activity in plasma and vacuolar membrane of corn seedling roots under salt stress conditions.
[So] Source:Ukr Biochem J;89(1):76-81, 2017 Jan-Feb.
[Is] ISSN:2409-4943
[Cp] País de publicação:Ukraine
[La] Idioma:eng
[Ab] Resumo:Ca2+-ATPases regulate the functioning of Ca2+-dependent signaling pathway SOS which provides removal of Na+ from the cytoplasm of cells via Na+/H+-antiporters in saline conditions. The influence of synthetic preparations Methyure and Ivine on the Ca2+-ATPase activity was investigated. It was shown that exposition of corn seedlings in the presence of 0.1 M NaCl rather enhanced hydrolytic than transport activity of Ca2+-ATPases in plasma and vacuolar membrane of root cells. It was found that seed treatment with such preparations, especially Methyure, caused intensification of the both activities of Ca2+-ATPases, mainly in vacuolar membrane. The results indicate than salt protective activity of preparations, especially Methyure, is associated with increased Ca2+-ATPase activity, which regulates the functioning of Na+/H+-antiporters.
[Mh] Termos MeSH primário: ATPases Transportadoras de Cálcio/metabolismo
Membrana Celular/efeitos dos fármacos
Substâncias Protetoras/farmacologia
Pirimidinas/farmacologia
Trocadores de Sódio-Hidrogênio/metabolismo
Vacúolos/efeitos dos fármacos
Zea mays/efeitos dos fármacos
[Mh] Termos MeSH secundário: Adaptação Fisiológica
Membrana Celular/metabolismo
Membranas Intracelulares/efeitos dos fármacos
Membranas Intracelulares/metabolismo
Transporte de Íons
Células Vegetais/efeitos dos fármacos
Células Vegetais/metabolismo
Raízes de Plantas/efeitos dos fármacos
Raízes de Plantas/metabolismo
Salinidade
Plântulas/efeitos dos fármacos
Plântulas/metabolismo
Cloreto de Sódio/farmacologia
Trocadores de Sódio-Hidrogênio/agonistas
Estresse Fisiológico
Vacúolos/metabolismo
Zea mays/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Protective Agents); 0 (Pyrimidines); 0 (Sodium-Hydrogen Exchangers); 451W47IQ8X (Sodium Chloride); EC 3.6.3.8 (Calcium-Transporting ATPases)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.15407/ubj89.01.076


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[PMID]:29227610
[Au] Autor:Kovalenko NO; Palladina TA
[Ti] Título:Gene expression of H+-pumps in plasma and vacuolar membranes of corn root cells under the effect of sodium ions and bioactive preparations.
[So] Source:Ukr Biochem J;88(2):89-97, 2016 Mar-Apr.
[Is] ISSN:2409-4943
[Cp] País de publicação:Ukraine
[La] Idioma:eng
[Ab] Resumo:Four isoforms of H+-ATPase of plasma membrane: MHA1, MHA2, MHA3, MHA4 are expressed in the corn seedling roots with prevalence of genes MHA3 і MHA4. The exposure of seedlings in the presence of 0.1 M NaCl activated the expression of MHA4 gene isoform, that demonstrates its important role in the processes of adaptation to salinization conditions. In vacuolar membrane, where potential is created by two Н+-pumps, sodium ions activated gene expression of only Н+-АТРase of V-type, taking no effect on the expression of Н+-pyrophosphatase. The seeds pretreatment by synthetic preparations Methyure and Ivine did not affect gene expression of Н+-pumps. Thus we can suppose that the ability of the above preparations to activate functioning of Н+-pumps in the presence of sodium ions is realized at the post-tranlation level.
[Mh] Termos MeSH primário: Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Proteínas de Plantas/genética
Raízes de Plantas/efeitos dos fármacos
Substâncias Protetoras/farmacologia
Pirimidinas/farmacologia
Cloreto de Sódio/farmacologia
ATPases Vacuolares Próton-Translocadoras/genética
[Mh] Termos MeSH secundário: Adaptação Fisiológica/genética
Membrana Celular/efeitos dos fármacos
Membrana Celular/enzimologia
Membranas Intracelulares/efeitos dos fármacos
Membranas Intracelulares/enzimologia
Isoenzimas/genética
Isoenzimas/metabolismo
Células Vegetais/efeitos dos fármacos
Células Vegetais/enzimologia
Proteínas de Plantas/metabolismo
Raízes de Plantas/enzimologia
Raízes de Plantas/crescimento & desenvolvimento
Salinidade
Plântulas/efeitos dos fármacos
Plântulas/enzimologia
Plântulas/crescimento & desenvolvimento
Sódio/metabolismo
ATPases Vacuolares Próton-Translocadoras/metabolismo
Vacúolos/efeitos dos fármacos
Vacúolos/enzimologia
Zea mays/efeitos dos fármacos
Zea mays/enzimologia
Zea mays/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Isoenzymes); 0 (Plant Proteins); 0 (Protective Agents); 0 (Pyrimidines); 451W47IQ8X (Sodium Chloride); 9NEZ333N27 (Sodium); EC 3.6.1.- (Vacuolar Proton-Translocating ATPases)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171212
[St] Status:MEDLINE
[do] DOI:10.15407/ubj88.02.089


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[PMID]:29197696
[Au] Autor:Alejandri-Ramírez ND; Chávez-Hernández EC; Contreras-Guerra JL; Reyes JL; Dinkova TD
[Ad] Endereço:Departamento de Bioquímica, Facultad de Química, Universidad Nacional Autónoma de México, 04510 Ciudad de México, Mexico.
[Ti] Título:Small RNA differential expression and regulation in Tuxpeño maize embryogenic callus induction and establishment.
[So] Source:Plant Physiol Biochem;122:78-89, 2018 Jan.
[Is] ISSN:1873-2690
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Somatic embryogenesis represents an alternative developmental process used to achieve genetic transformation and to approach key questions in maize development. It is known that embryogenic callus induction and plant regeneration are accompanied by microRNA expression changes. However, small RNA (sRNA) populations have not been explored during the proliferative callus subculture establishment and their impact on maintaining the dedifferentiated status and embryogenic potential is far from being completely understood. Here we globally tested the sRNA populations in explants (immature embryos), induced and established maize embryogenic callus from the Mexican cultivar VS-535, Tuxpeño landrace. We detected readjustments in 24 nt and 21-22 nt sRNAs during the embryogenic callus (EC) establishment and maintenance. A follow up on specific microRNAs (miRNAs) indicated that miRNAs related to stress response substantially increase upon the callus proliferation establishment, correlating with a reduction in some of their target levels. On the other hand, while 24 nt-long heterochromatic small interfering RNAs (hc-siRNAs) derived from transposable retroelements transiently decreased in abundance during the EC establishment, a population of 22 nt-hc-siRNAs increased. This was accompanied by reduction in transposon expression in the established callus subcultures. We conclude that stress- and development-related miRNAs are highly expressed upon maize EC callus induction and during maintenance of the subcultures, while miRNAs involved in hormone response only transiently increase during induction. In addition, the establishment of a proliferative status in embryogenic callus is accompanied by important readjustments in hc-siRNAs mapping to long tandem repeat (LTR) retrotransposons, and their expression regulation.
[Mh] Termos MeSH primário: Regulação da Expressão Gênica de Plantas
MicroRNAs/biossíntese
Células Vegetais/metabolismo
Técnicas de Embriogênese Somática de Plantas
RNA de Plantas/biossíntese
Zea mays/metabolismo
[Mh] Termos MeSH secundário: Zea mays/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MicroRNAs); 0 (RNA, Plant)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171204
[St] Status:MEDLINE


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[PMID]:28449656
[Au] Autor:Grant JN; Burris JN; Stewart CN; Lenaghan SC
[Ad] Endereço:Department of Plant Science, University of Tennessee, 2431 Joe Johnson Drive, Knoxville, TN, 37996, USA.
[Ti] Título:Improved tissue culture conditions for the emerging C model Panicum hallii.
[So] Source:BMC Biotechnol;17(1):39, 2017 04 27.
[Is] ISSN:1472-6750
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Panicum hallii Vasey (Hall's panicgrass) is a compact, perennial C grass in the family Poaceae, which has potential to enable bioenergy research for switchgrass (Panicum virgatum L.). Unlike P. hallii, switchgrass has a large genome, allopolyploidy, self-incompatibility, a long life cycle, and large stature-all suboptimal traits for rapid genetics research. Herein we improved tissue culture methodologies for two inbred P. hallii populations: FIL2 and HAL2, to enable further development of P. hallii as a model C plant. RESULTS: The optimal seed-derived callus induction medium was determined to be Murashige and Skoog (MS) medium supplemented with 40 mg L L-cysteine, 300 mg L L-proline, 3% sucrose, 1 g L casein hydrolysate, 3 mg L 2,4-dichlorophenoxyacetic acid (2,4-D), and 45 µg L 6-benzylaminopurine (BAP), which resulted in callus induction of 51 ± 29% for FIL2 and 81 ± 19% for HAL2. The optimal inflorescence-derived callus induction was observed on MP medium (MS medium supplemented with 2 g L L-proline, 3% maltose, 5 mg L 2,4-D, and 500 µg L BAP), resulting in callus induction of 100 ± 0.0% for FIL2 and 84 ± 2.4% for HAL2. Shoot regeneration rates of 11.5 ± 0.8 shoots/gram for FIL2 and 11.3 ± 0.6 shoots/gram for HAL2 were achieved using seed-induced callus, whereas shoot regeneration rates of 26.2 ± 2.6 shoots/gram for FIL2 and 29.3 ± 3.6 shoots/gram for HAL2 were achieved from inflorescence-induced callus. Further, cell suspension cultures of P. hallii were established from seed-derived callus, providing faster generation of callus tissue compared with culture using solidified media (1.41-fold increase for FIL2 and 3.00-fold increase for HAL2). CONCLUSIONS: Aside from abbreviated tissue culture times from callus induction to plant regeneration for HAL2, we noted no apparent differences between FIL2 and HAL2 populations in tissue culture performance. For both populations, the cell suspension cultures outperformed tissue cultures on solidified media. Using the methods developed in this work, P. hallii callus was induced from seeds immediately after harvest in a shorter time and with higher frequencies than switchgrass. For clonal propagation, P. hallii callus was established from R1 inflorescences, similar to switchgrass, which further strengthens the potential of this plant as a C model for genetic studies. The rapid cycling (seed-to-seed time) and ease of culture, further demonstrate the potential utility of P. hallii as a C model plant.
[Mh] Termos MeSH primário: Meios de Cultura/química
Panicum/crescimento & desenvolvimento
Técnicas de Cultura de Tecidos/métodos
[Mh] Termos MeSH secundário: Meios de Cultura/farmacologia
Germinação/efeitos dos fármacos
Inflorescência/crescimento & desenvolvimento
Modelos Biológicos
Células Vegetais/efeitos dos fármacos
Células Vegetais/fisiologia
Sementes/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Culture Media)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1186/s12896-017-0359-0


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[PMID]:27773616
[Au] Autor:Malhotra K; Subramaniyan M; Rawat K; Kalamuddin M; Qureshi MI; Malhotra P; Mohmmed A; Cornish K; Daniell H; Kumar S
[Ad] Endereço:Metabolic Engineering Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India.
[Ti] Título:Compartmentalized Metabolic Engineering for Artemisinin Biosynthesis and Effective Malaria Treatment by Oral Delivery of Plant Cells.
[So] Source:Mol Plant;9(11):1464-1477, 2016 11 07.
[Is] ISSN:1752-9867
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Artemisinin is highly effective against drug-resistant malarial parasites, which affects nearly half of the global population and kills >500 000 people each year. The primary cost of artemisinin is the very expensive process used to extract and purify the drug from Artemisia annua. Elimination of this apparently unnecessary step will make this potent antimalarial drug affordable to the global population living in endemic regions. Here we reported the oral delivery of a non-protein drug artemisinin biosynthesized (∼0.8 mg/g dry weight) at clinically meaningful levels in tobacco by engineering two metabolic pathways targeted to three different cellular compartments (chloroplast, nucleus, and mitochondria). The doubly transgenic lines showed a three-fold enhancement of isopentenyl pyrophosphate, and targeting AACPR, DBR2, and CYP71AV1 to chloroplasts resulted in higher expression and an efficient photo-oxidation of dihydroartemisinic acid to artemisinin. Partially purified extracts from the leaves of transgenic tobacco plants inhibited in vitro growth progression of Plasmodium falciparum-infected red blood cells. Oral feeding of whole intact plant cells bioencapsulating the artemisinin reduced the parasitemia levels in challenged mice in comparison with commercial drug. Such novel synergistic approaches should facilitate low-cost production and delivery of artemisinin and other drugs through metabolic engineering of edible plants.
[Mh] Termos MeSH primário: Artemisininas/metabolismo
Artemisininas/farmacologia
Malária Falciparum/tratamento farmacológico
Engenharia Metabólica
Células Vegetais/metabolismo
[Mh] Termos MeSH secundário: Administração Oral
Animais
Artemisininas/uso terapêutico
Cloroplastos/genética
Camundongos
Plantas Geneticamente Modificadas
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Artemisinins); 9RMU91N5K2 (artemisinine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171213
[Lr] Data última revisão:
171213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


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[PMID]:28838936
[Au] Autor:Zeng J; Dong Z; Wu H; Tian Z; Zhao Z
[Ad] Endereço:School of Life Sciences, University of Science and Technology of China, Hefei, China.
[Ti] Título:Redox regulation of plant stem cell fate.
[So] Source:EMBO J;36(19):2844-2855, 2017 Oct 02.
[Is] ISSN:1460-2075
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Despite the importance of stem cells in plant and animal development, the common mechanisms of stem cell maintenance in both systems have remained elusive. Recently, the importance of hydrogen peroxide (H O ) signaling in priming stem cell differentiation has been extensively studied in animals. Here, we show that different forms of reactive oxygen species (ROS) have antagonistic roles in plant stem cell regulation, which were established by distinct spatiotemporal patterns of ROS-metabolizing enzymes. The superoxide anion (O2·-) is markedly enriched in stem cells to activate and maintain stemness, whereas H O is more abundant in the differentiating peripheral zone to promote stem cell differentiation. Moreover, H O negatively regulates O2·- biosynthesis in stem cells, and increasing H O levels or scavenging O2·- leads to the termination of stem cells. Our results provide a mechanistic framework for ROS-mediated control of plant stem cell fate and demonstrate that the balance between O2·- and H O is key to stem cell maintenance and differentiation.
[Mh] Termos MeSH primário: Diferenciação Celular/efeitos dos fármacos
Peróxido de Hidrogênio/farmacologia
Células Vegetais/efeitos dos fármacos
Células Vegetais/fisiologia
Células-Tronco/efeitos dos fármacos
Células-Tronco/fisiologia
Superóxidos/farmacologia
[Mh] Termos MeSH secundário: Arabidopsis/efeitos dos fármacos
Arabidopsis/genética
Arabidopsis/crescimento & desenvolvimento
Germinação/efeitos dos fármacos
Peróxido de Hidrogênio/metabolismo
Oxirredução/efeitos dos fármacos
Desenvolvimento Vegetal/efeitos dos fármacos
Plantas Geneticamente Modificadas
Espécies Reativas de Oxigênio/metabolismo
Espécies Reativas de Oxigênio/farmacologia
Transdução de Sinais/efeitos dos fármacos
Transdução de Sinais/fisiologia
Nicho de Células-Tronco/efeitos dos fármacos
Superóxido Dismutase/metabolismo
Superóxidos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Reactive Oxygen Species); 11062-77-4 (Superoxides); BBX060AN9V (Hydrogen Peroxide); EC 1.15.1.1 (Superoxide Dismutase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170826
[St] Status:MEDLINE
[do] DOI:10.15252/embj.201695955


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[PMID]:28700920
[Au] Autor:Lin C; White RR; Sparkes I; Ashwin P
[Ad] Endereço:Center for Mathematical Sciences, Huazhong University of Science and Technology, Wuhan, China; Hubei Key Lab of Engineering Modeling and Scientific Computing, Huazhong University of Science and Technology, Wuhan, China; Department of Mathematics, University of Exeter, Exeter, United Kingdom. Electro
[Ti] Título:Modeling Endoplasmic Reticulum Network Maintenance in a Plant Cell.
[So] Source:Biophys J;113(1):214-222, 2017 Jul 11.
[Is] ISSN:1542-0086
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The endoplasmic reticulum (ER) in plant cells forms a highly dynamic network of complex geometry. ER network morphology and dynamics are influenced by a number of biophysical processes, including filament/tubule tension, viscous forces, Brownian diffusion, and interactions with many other organelles and cytoskeletal elements. Previous studies have indicated that ER networks can be thought of as constrained minimal-length networks acted on by a variety of forces that perturb and/or remodel the network. Here, we study two specific biophysical processes involved in remodeling. One is the dynamic relaxation process involving a combination of tubule tension and viscous forces. The other is the rapid creation of cross-connection tubules by direct or indirect interactions with cytoskeletal elements. These processes are able to remodel the ER network: the first reduces network length and complexity whereas the second increases both. Using live cell imaging of ER network dynamics in tobacco leaf epidermal cells, we examine these processes on ER network dynamics. Away from regions of cytoplasmic streaming, we suggest that the dynamic network structure is a balance between the two processes, and we build an integrative model of the two processes for network remodeling. This model produces quantitatively similar ER networks to those observed in experiments. We use the model to explore the effect of parameter variation on statistical properties of the ER network.
[Mh] Termos MeSH primário: Retículo Endoplasmático/metabolismo
Modelos Biológicos
Células Vegetais/metabolismo
[Mh] Termos MeSH secundário: Agrobacterium
Corrente Citoplasmática/fisiologia
Proteínas de Fluorescência Verde/genética
Proteínas de Fluorescência Verde/metabolismo
Proteínas Luminescentes/genética
Proteínas Luminescentes/metabolismo
Microscopia Confocal
Folhas de Planta/citologia
Folhas de Planta/metabolismo
Análise de Célula Única
Tabaco/citologia
Tabaco/metabolismo
Transformação Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES; VIDEO-AUDIO MEDIA
[Nm] Nome de substância:
0 (Luminescent Proteins); 0 (red fluorescent protein); 147336-22-9 (Green Fluorescent Proteins)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170825
[Lr] Data última revisão:
170825
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170713
[St] Status:MEDLINE


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[PMID]:28698411
[Au] Autor:Durand-Smet P; Gauquelin E; Chastrette N; Boudaoud A; Asnacios A
[Ad] Endereço:Laboratoire Matière et Systèmes Complexes, UMR 7057 CNRS & Université Paris Diderot, Sorbonne Paris Cité, Paris, France.
[Ti] Título:Estimation of turgor pressure through comparison between single plant cell and pressurized shell mechanics.
[So] Source:Phys Biol;14(5):055002, 2017 Aug 16.
[Is] ISSN:1478-3975
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:While plant growth is well known to rely on turgor pressure, it is challenging to quantify the contribution of turgor pressure to plant cell rheology. Here we used a custom-made micro-rheometer to quantify the viscoelastic behavior of isolated plant cells while varying their internal turgor pressure. To get insight into how plant cells adapt their internal pressure to the osmolarity of their medium, we compared the mechanical behavior of single plant cells to that of a simple, passive, pressurized shell: a soccer ball. While both systems exhibited the same qualitative behavior, a simple mechanical model allowed us to quantify turgor pressure regulation at the single cell scale.
[Mh] Termos MeSH primário: Pressão Osmótica
Células Vegetais
[Mh] Termos MeSH secundário: Fenômenos Biomecânicos
Elasticidade
Modelos Biológicos
Reologia
Viscosidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170830
[Lr] Data última revisão:
170830
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170713
[St] Status:MEDLINE
[do] DOI:10.1088/1478-3975/aa7f30



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