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Pesquisa : A11.870.700 [Categoria DeCS]
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  1 / 10106 MEDLINE  
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[PMID]:28461450
[Au] Autor:Rothstein DM; Lazinski D; Osburne MS; Sonenshein AL
[Ad] Endereço:Graduate Program in Molecular Biology, Tufts University School of Medicine, Boston, Massachusetts, USA.
[Ti] Título:A Mutation in the Bacillus subtilis rsbU Gene That Limits RNA Synthesis during Sporulation.
[So] Source:J Bacteriol;199(14), 2017 07 15.
[Is] ISSN:1098-5530
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mutants of that are temperature sensitive for RNA synthesis during sporulation were isolated after selection with a P suicide agent. Whole-genome sequencing revealed that two of the mutants carried an identical lesion in the gene, which encodes a phosphatase that indirectly activates SigB, the stress-responsive RNA polymerase sigma factor. The mutation appeared to cause RsbU to be hyperactive, because the mutants were more resistant than the parent strain to ethanol stress. In support of this hypothesis, pseudorevertants that regained wild-type levels of sporulation at high temperature had secondary mutations that prevented expression of the mutant gene. The properties of these RsbU mutants support the idea that activation of SigB diminishes the bacterium's ability to sporulate. Most bacterial species encode multiple RNA polymerase promoter recognition subunits (sigma factors). Each sigma factor directs RNA polymerase to different sets of genes; each gene set typically encodes proteins important for responses to specific environmental conditions, such as changes in temperature, salt concentration, and nutrient availability. A selection for mutants of that are temperature sensitive for RNA synthesis during sporulation unexpectedly yielded strains with a point mutation in , a gene that encodes a protein that normally activates sigma factor B (SigB) under conditions of salt stress. The mutation appears to cause RsbU, and therefore SigB, to be active inappropriately, thereby inhibiting, directly or indirectly, the ability of the cells to transcribe sporulation genes.
[Mh] Termos MeSH primário: Bacillus subtilis/metabolismo
Proteínas de Bactérias/metabolismo
Regulação Bacteriana da Expressão Gênica/fisiologia
Monoéster Fosfórico Hidrolases/metabolismo
RNA Bacteriano/biossíntese
Esporos Bacterianos/fisiologia
[Mh] Termos MeSH secundário: Bacillus subtilis/genética
Proteínas de Bactérias/genética
Etanol/farmacologia
Genoma Bacteriano
Temperatura Alta
Mutação
Fosfatos/metabolismo
Monoéster Fosfórico Hidrolases/genética
Radioisótopos de Fósforo
Estresse Fisiológico/efeitos dos fármacos
Estresse Fisiológico/efeitos da radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Phosphates); 0 (Phosphorus Radioisotopes); 0 (RNA, Bacterial); 3K9958V90M (Ethanol); EC 3.1.3.2 (Phosphoric Monoester Hydrolases); EC 3.1.3.3 (RsbU protein, Bacillus subtilis)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE


  2 / 10106 MEDLINE  
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[PMID]:29302032
[Au] Autor:Mutlu A; Trauth S; Ziesack M; Nagler K; Bergeest JP; Rohr K; Becker N; Höfer T; Bischofs IB
[Ad] Endereço:BioQuant Center of the University of Heidelberg, 69120, Heidelberg, Germany.
[Ti] Título:Phenotypic memory in Bacillus subtilis links dormancy entry and exit by a spore quantity-quality tradeoff.
[So] Source:Nat Commun;9(1):69, 2018 01 04.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Some bacteria, such as Bacillus subtilis, withstand starvation by forming dormant spores that revive when nutrients become available. Although sporulation and spore revival jointly determine survival in fluctuating environments, the relationship between them has been unclear. Here we show that these two processes are linked by a phenotypic "memory" that arises from a carry-over of molecules from the vegetative cell into the spore. By imaging life histories of individual B. subtilis cells using fluorescent reporters, we demonstrate that sporulation timing controls nutrient-induced spore revival. Alanine dehydrogenase contributes to spore memory and controls alanine-induced outgrowth, thereby coupling a spore's revival capacity to the gene expression and growth history of its progenitors. A theoretical analysis, and experiments with signaling mutants exhibiting altered sporulation timing, support the hypothesis that such an intrinsically generated memory leads to a tradeoff between spore quantity and spore quality, which could drive the emergence of complex microbial traits.
[Mh] Termos MeSH primário: Bacillus subtilis/genética
Regulação Bacteriana da Expressão Gênica
Mutação
Esporos Bacterianos/genética
[Mh] Termos MeSH secundário: Alanina Desidrogenase/genética
Alanina Desidrogenase/metabolismo
Algoritmos
Bacillus subtilis/metabolismo
Bacillus subtilis/fisiologia
Fenômenos Fisiológicos Bacterianos/genética
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Modelos Genéticos
Esporos Bacterianos/crescimento & desenvolvimento
Esporos Bacterianos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); EC 1.4.1.1 (Alanine Dehydrogenase)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180106
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02477-1


  3 / 10106 MEDLINE  
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[PMID]:28744555
[Au] Autor:Sultanpuram VR; Mothe T; Chintalapati S; Chintalapati VR
[Ad] Endereço:Microbial Ecology Laboratory, Department of Applied Biosciences, Mahatma Gandhi University, Anneparthy, Yellareddygudem (PO), Nalgonda, 508254, India. svr.micro@gmail.com.
[Ti] Título:Bacillus catenulatus sp. nov., an alkalitolerant bacterium isolated from a soda lake.
[So] Source:Arch Microbiol;199(10):1391-1397, 2017 Dec.
[Is] ISSN:1432-072X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Two novel (18C and 6C) Gram-stain-positive, rod shaped, motile and endospore-forming bacterial strains were isolated from Lonar soda lake, India. Based on 16S rRNA gene sequence analysis, strains 18C and 6C were identified as belonging to the class Firmibacteria, and were most closely related to Bacillus cohnii KCTC 3572 (99.3 and 99.9%, respectively), Bacillus zhanjiangensis KCTC 13713 (97.4 and 98.0%, respectively), Bacillus halmapalus LMG 17950 (97.0 and 97.6%, respectively) and other members in the genus Bacillus (<97.0%). However, the DNA-DNA relatedness between 18C and 6C and B. cohnii KCTC 3572 (49.6 ± 0.9 and 51.6 ± 0.7, respectively), B. zhanjiangensis KCTC 13713 (42.9 ± 0.8 and 47.1 ± 0.3, respectively) and B. halmapalus LMG 17950 (39.9 ± 0.8 and 40.8 ± 0.3, respectively) indicated that the novel strains were distantly related to these strains. Further, the high 16S rRNA gene sequence similarity (100%) and DNA-DNA relatedness (90 ± 5%) suggested that strains 18C and 6C were members of a genomospecies. The strains grew optimally at a pH of 7.5 with 2-3% (w/v) NaCl and temperature of 37 °C. Strains 18C and 6C were catalase and oxidase negative. The cell wall of strain 18C contained meso-diaminopimelic acid as the diagnostic diamino acid, which was in contrast with its nearest neighbour B. cohnii KCTC 3572 , which contained ornithine and aspartic acid. Polar lipids include diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), an unknown phospholipid (PL) and three unknown lipids (L1-3). The predominant isoprenoid quinone was MK-7. iso-C (32.5%) was the predominant fatty acid and significant proportions of anteiso-C (19.5%), C (11.5%), iso-C (9.5%) and anteiso-C (6.3%) were also detected. The DNA G + C content of strains 18C and 6C were 39.3 and 39.2 mol%, respectively. The results of molecular, biochemical and chemotaxonomic tests showed a clear differentiation of strains 18C and 6C from all other members of the genus Bacillus, for which the name Bacillus catenulatus sp. nov. is proposed. The type strain is 18C (=KCTC 33781 = CGMCC 1.15475 ).
[Mh] Termos MeSH primário: Bacillus
Lagos/microbiologia
[Mh] Termos MeSH secundário: Bacillus/classificação
Bacillus/genética
Bacillus/isolamento & purificação
Técnicas de Tipagem Bacteriana
Composição de Bases/genética
Parede Celular/química
DNA Bacteriano/genética
Ácido Diaminopimélico/análise
Ácidos Graxos/análise
Índia
Hibridização de Ácido Nucleico
Peptidoglicano/química
Fosfatidiletanolaminas/análise
Fosfolipídeos/análise
Filogenia
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Cloreto de Sódio/análise
Esporos Bacterianos/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Peptidoglycan); 0 (Phosphatidylethanolamines); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 39382-08-6 (phosphatidylethanolamine); 451W47IQ8X (Sodium Chloride); 583-93-7 (Diaminopimelic Acid)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.1007/s00203-017-1413-y


  4 / 10106 MEDLINE  
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[PMID]:29351296
[Au] Autor:Passera A; Marcolungo L; Casati P; Brasca M; Quaglino F; Cantaloni C; Delledonne M
[Ad] Endereço:Department of Agricultural and Environmental Sciences - Production, Landscape, Agroenergy, Università degli Studi di Milano, Milan, Italy.
[Ti] Título:Hybrid genome assembly and annotation of Paenibacillus pasadenensis strain R16 reveals insights on endophytic life style and antifungal activity.
[So] Source:PLoS One;13(1):e0189993, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bacteria of the Paenibacillus genus are becoming important in many fields of science, including agriculture, for their positive effects on the health of plants. However, there are little information available on this genus compared to other bacteria (such as Bacillus or Pseudomonas), especially when considering genomic information. Sequencing the genomes of plant-beneficial bacteria is a crucial step to identify the genetic elements underlying the adaptation to life inside a plant host and, in particular, which of these features determine the differences between a helpful microorganism and a pathogenic one. In this study, we have characterized the genome of Paenibacillus pasadenensis, strain R16, recently investigated for its antifungal activities and plant-associated features. An hybrid assembly approach was used integrating the very precise reads obtained by Illumina technology and long fragments acquired with Oxford Nanopore Technology (ONT) sequencing. De novo genome assembly based solely on Illumina reads generated a relatively fragmented assembly of 5.72 Mbp in 99 ungapped sequences with an N50 length of 544 Kbp; hybrid assembly, integrating Illumina and ONT reads, improved the assembly quality, generating a genome of 5.75 Mbp, organized in 6 contigs with an N50 length of 3.4 Mbp. Annotation of the latter genome identified 4987 coding sequences, of which 1610 are hypothetical proteins. Enrichment analysis identified pathways of particular interest for the endophyte biology, including the chitin-utilization pathway and the incomplete siderophore pathway which hints at siderophore parasitism. In addition the analysis led to the identification of genes for the production of terpenes, as for example farnesol, that was hypothesized as the main antifungal molecule produced by the strain. The functional analysis on the genome confirmed several plant-associated, plant-growth promotion, and biocontrol traits of strain R16, thus adding insights in the genetic bases of these complex features, and of the Paenibacillus genus in general.
[Mh] Termos MeSH primário: Endófitos/genética
Fungos/patogenicidade
Genoma Bacteriano
Paenibacillus/genética
Plantas/microbiologia
[Mh] Termos MeSH secundário: Amino Açúcares/metabolismo
Metabolismo dos Carboidratos
DNA Bacteriano/genética
DNA Bacteriano/isolamento & purificação
Endófitos/metabolismo
Endófitos/fisiologia
Sequenciamento de Nucleotídeos em Larga Escala
Compostos Orgânicos/metabolismo
Paenibacillus/metabolismo
Paenibacillus/fisiologia
Desenvolvimento Vegetal
Sideróforos/biossíntese
Esporos Bacterianos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Sugars); 0 (DNA, Bacterial); 0 (Organic Chemicals); 0 (Siderophores)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180120
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189993


  5 / 10106 MEDLINE  
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[PMID]:29293521
[Au] Autor:Cambridge JM; Blinkova AL; Salvador Rocha EI; Bode Hernández A; Moreno M; Ginés-Candelaria E; Goetz BM; Hunicke-Smith S; Satterwhite E; Tucker HO; Walker JR
[Ad] Endereço:Department of Molecular Biosciences and Institute for Cell and Molecular Biology, University of Texas, Austin, TX, United States of America.
[Ti] Título:Genomics of Clostridium taeniosporum, an organism which forms endospores with ribbon-like appendages.
[So] Source:PLoS One;13(1):e0189673, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Clostridium taeniosporum, a non-pathogenic anaerobe closely related to the C. botulinum Group II members, was isolated from Crimean lake silt about 60 years ago. Its endospores are surrounded by an encasement layer which forms a trunk at one spore pole to which about 12-14 large, ribbon-like appendages are attached. The genome consists of one 3,264,813 bp, circular chromosome (with 26.6% GC) and three plasmids. The chromosome contains 2,892 potential protein coding sequences: 2,124 have specific functions, 147 have general functions, 228 are conserved but without known function and 393 are hypothetical based on the fact that no statistically significant orthologs were found. The chromosome also contains 101 genes for stable RNAs, including 7 rRNA clusters. Over 84% of the protein coding sequences and 96% of the stable RNA coding regions are oriented in the same direction as replication. The three known appendage genes are located within a single cluster with five other genes, the protein products of which are closely related, in terms of sequence, to the known appendage proteins. The relatedness of the deduced protein products suggests that all or some of the closely related genes might code for minor appendage proteins or assembly factors. The appendage genes might be unique among the known clostridia; no statistically significant orthologs were found within other clostridial genomes for which sequence data are available. The C. taeniosporum chromosome contains two functional prophages, one Siphoviridae and one Myoviridae, and one defective prophage. Three plasmids of 5.9, 69.7 and 163.1 Kbp are present. These data are expected to contribute to future studies of developmental, structural and evolutionary biology and to potential industrial applications of this organism.
[Mh] Termos MeSH primário: Clostridium/genética
Genoma Bacteriano
Esporos Bacterianos
[Mh] Termos MeSH secundário: Clostridium/metabolismo
Filogenia
Prófagos/classificação
Prófagos/genética
Origem de Replicação
Selenoproteínas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Selenoproteins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189673


  6 / 10106 MEDLINE  
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[PMID]:28457517
[Au] Autor:Akasaka K; Maeno A; Yamazaki A
[Ad] Endereço:Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, 1-5 Hangi-cho, Shimogamo, Sakyo-ku, Kyoto 606-8522, Japan; Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kamigyo-ku, Kyoto 602-8566, Japan. Electronic address: akasakak@koto.kpu-m.ac.jp.
[Ti] Título:Direct high-pressure NMR observation of dipicolinic acid leaking from bacterial spore: A crucial step for thermal inactivation.
[So] Source:Biophys Chem;231:10-14, 2017 Dec.
[Is] ISSN:1873-4200
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A bacterial spore protects itself with an unusually high concentration (~10% in dry weight of spore) of dipicolinic acid (DPA), the release of which is considered the crucial step for inactivating it under mild pressure and temperature conditions. However, the process of how the spore releases DPA in response to pressure remains obscure. Here we apply H high-resolution high-pressure NMR spectroscopy, for the first time, to the spore suspension of Bacillus subtilis natto and monitor directly and in real-time the leaking process of DPA in response to pressure of 200MPa at 20°C. We find that about one third of the total DPA leaks immediately upon applying pressure, but that the rest leaks slowly in hrs upon decreasing the pressure. Once DPA is fully released from the spore, the proteins of the spore become easily denatured at a mild temperature, e.g., 80°C, much below the temperature commonly used to inactivate spores (121°C). The success of the present experiment opens a new avenue for studying bacterial spores and cells at the molecular level in response to pressure, temperature and other perturbations.
[Mh] Termos MeSH primário: Bacillus subtilis/fisiologia
Ácidos Picolínicos/metabolismo
Esporos Bacterianos/fisiologia
[Mh] Termos MeSH secundário: Temperatura Alta
Espectroscopia de Ressonância Magnética
Pressão
Solubilidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Picolinic Acids); UE81S5CQ0G (dipicolinic acid)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171229
[Lr] Data última revisão:
171229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


  7 / 10106 MEDLINE  
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[PMID]:29073204
[Au] Autor:Maier MB; Lenz CA; Vogel RF
[Ad] Endereço:Lehrstuhl für Technische Mikrobiologie, Technische Universität München, Freising, Germany.
[Ti] Título:Non-linear pressure/temperature-dependence of high pressure thermal inactivation of proteolytic Clostridium botulinum type B in foods.
[So] Source:PLoS One;12(10):e0187023, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The effect of high pressure thermal (HPT) processing on the inactivation of spores of proteolytic type B Clostridium botulinum TMW 2.357 in four differently composed low-acid foods (green peas with ham, steamed sole, vegetable soup, braised veal) was studied in an industrially feasible pressure range and temperatures between 100 and 120°C. Inactivation curves exhibited rapid inactivation during compression and decompression followed by strong tailing effects. The highest inactivation (approx. 6-log cycle reduction) was obtained in braised veal at 600 MPa and 110°C after 300 s pressure-holding time. In general, inactivation curves exhibited similar negative exponential shapes, but maximum achievable inactivation levels were lower in foods with higher fat contents. At high treatment temperatures, spore inactivation was more effective at lower pressure levels (300 vs. 600 MPa), which indicates a non-linear pressure/temperature-dependence of the HPT spore inactivation efficiency. A comparison of spore inactivation levels achievable using HPT treatments versus a conventional heat sterilization treatment (121.1°C, 3 min) illustrates the potential of combining high pressures and temperatures to replace conventional retorting with the possibility to reduce the process temperature or shorten the processing time. Finally, experiments using varying spore inoculation levels suggested the presence of a resistant fraction comprising approximately 0.01% of a spore population as reason for the pronounced tailing effects in survivor curves. The loss of the high resistance properties upon cultivation indicates that those differences develop during sporulation and are not linked to permanent modifications at the genetic level.
[Mh] Termos MeSH primário: Clostridium botulinum tipo B/fisiologia
Microbiologia de Alimentos
Temperatura Alta
Viabilidade Microbiana
Dinâmica não Linear
Pressão
[Mh] Termos MeSH secundário: Esporos Bacterianos/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171110
[Lr] Data última revisão:
171110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171027
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0187023


  8 / 10106 MEDLINE  
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[PMID]:29065168
[Au] Autor:Black E; Owens K; Staub R; Li J; Mills K; Valenstein J; Hilgren J
[Ad] Endereço:Ecolab Research, Development and Engineering Center, Eagan, Minnesota, United States of America.
[Ti] Título:Evaluation of AISI Type 304 stainless steel as a suitable surface material for evaluating the efficacy of peracetic acid-based disinfectants against Clostridium difficile spores.
[So] Source:PLoS One;12(10):e0187074, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Disinfectants play an important role in controlling microbial contamination on hard surfaces in hospitals. The effectiveness of disinfectants in real life can be predicted by laboratory tests that measure killing of microbes on carriers. The modified Quantitative Disk Carrier Test (QCT-2) is a standard laboratory method that employs American Iron and Steel Institute (AISI) Type 430 stainless steel carriers to measure hospital disinfectant efficacy against Clostridium difficile spores. The formation of a rust-colored precipitate was observed on Type 430 carriers when testing a peracetic acid (PAA)-based disinfectant with the QCT-2 method. It was hypothesized that the precipitate was indicative of corrosion of the Type 430 carrier, and that corrosion could impact efficacy results. The objective of this study was to compare the suitability of AISI Type 430 to Type 304 stainless steel carriers for evaluating PAA-based disinfectants using the QCT-2 method. Type 304 is more corrosion-resistant than Type 430, is ubiquitous in healthcare environments, and is used in other standard methods. Suitability of the carriers was evaluated by comparing their impacts on efficacy results and PAA degradation rates. In efficacy tests with 1376 ppm PAA, reductions of C. difficile spores after 5, 7 and 10 minutes on Type 430 carriers were at least about 1.5 log10 lower than reductions on Type 304 carriers. In conditions simulating a QCT-2 test, PAA concentration with Type 430 carriers was reduced by approximately 80% in 10 minutes, whereas PAA concentration in the presence of Type 304 carriers remained stable. Elemental analyses of residues on each carrier type after efficacy testing were indicative of corrosion on the Type 430 carrier. Use of Type 430 stainless steel carriers for measuring the efficacy of PAA-based disinfectants should be avoided as it can lead to an underestimation of real life sporicidal efficacy. Type 304 stainless steel carriers are recommended as a suitable alternative.
[Mh] Termos MeSH primário: Clostridium difficile/crescimento & desenvolvimento
Desinfetantes/farmacologia
Ácido Peracético/farmacologia
Esporos Bacterianos/efeitos dos fármacos
Aço Inoxidável
[Mh] Termos MeSH secundário: Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Disinfectants); 12597-68-1 (Stainless Steel); I6KPI2E1HD (Peracetic Acid)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171025
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0187074


  9 / 10106 MEDLINE  
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[PMID]:28945739
[Au] Autor:Ramírez-Guadiana FH; Meeske AJ; Rodrigues CDA; Barajas-Ornelas RDC; Kruse AC; Rudner DZ
[Ad] Endereço:Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA, United States of America.
[Ti] Título:A two-step transport pathway allows the mother cell to nurture the developing spore in Bacillus subtilis.
[So] Source:PLoS Genet;13(9):e1007015, 2017 Sep.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:One of the hallmarks of bacterial endospore formation is the accumulation of high concentrations of pyridine-2,6-dicarboxylic acid (dipicolinic acid or DPA) in the developing spore. This small molecule comprises 5-15% of the dry weight of dormant spores and plays a central role in resistance to both wet heat and desiccation. DPA is synthesized in the mother cell at a late stage in sporulation and must be translocated across two membranes (the inner and outer forespore membranes) that separate the mother cell and forespore. The enzymes that synthesize DPA and the proteins required to translocate it across the inner forespore membrane were identified over two decades ago but the factors that transport DPA across the outer forespore membrane have remained mysterious. Here, we report that SpoVV (formerly YlbJ) is the missing DPA transporter. SpoVV is produced in the mother cell during the morphological process of engulfment and specifically localizes in the outer forespore membrane. Sporulating cells lacking SpoVV produce spores with low levels of DPA and cells engineered to express SpoVV and the DPA synthase during vegetative growth accumulate high levels of DPA in the culture medium. SpoVV resembles concentrative nucleoside transporters and mutagenesis of residues predicted to form the substrate-binding pocket supports the idea that SpoVV has a similar structure and could therefore function similarly. These findings provide a simple two-step transport mechanism by which the mother cell nurtures the developing spore. DPA produced in the mother cell is first translocated into the intermembrane space by SpoVV and is then imported into the forespore by the SpoVA complex. This pathway is likely to be broadly conserved as DPA synthase, SpoVV, and SpoVA proteins can be found in virtually all endospore forming bacteria.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Membrana Celular/genética
Proteínas de Membrana/genética
Ácidos Picolínicos/metabolismo
Esporos Bacterianos/genética
[Mh] Termos MeSH secundário: Bacillus subtilis/genética
Bacillus subtilis/crescimento & desenvolvimento
Transporte Biológico/genética
Membrana Celular/enzimologia
Dessecação
Temperatura Alta
Proteínas de Membrana/metabolismo
Esporos Bacterianos/enzimologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Membrane Proteins); 0 (Picolinic Acids); UE81S5CQ0G (dipicolinic acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171022
[Lr] Data última revisão:
171022
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170926
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1007015


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[PMID]:28917834
[Au] Autor:Xu Y; Jo I; Wang L; Chen J; Fan S; Dong Y; Quan C; Ha NC
[Ad] Endereço:Department of Bioengineering, College of Life Science, Dalian Minzu University, Dalian 116600, Liaoning, China; Key Laboratory of Biotechnology and Bioresources Utilization (Dalian Minzu University), Ministry of Education, China. Electronic address: yongbinxu@dlnu.edu.cn.
[Ti] Título:Hexameric assembly of membrane fusion protein YknX of the sporulation delaying efflux pump from Bacillus amyloliquefaciens.
[So] Source:Biochem Biophys Res Commun;493(1):152-157, 2017 Nov 04.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Membrane fusion proteins (MFPs) play an essential role in the action of the drug efflux pumps and protein secretion systems in bacteria. The sporulation delaying protein (SDP) efflux pump YknWXYZ has been identified in diverse Bacillus species. The MFP YknX requires the ATP-binding cassette (ABC) transporter YknYZ and the Yip1 family protein YknW to form a functional complex. To date, the crystal structure, molecular function and mechanism of action of YknX remain unknown. In this study, to characterize the structural and biochemical roles of YknX in the functional assembly of YknWXYZ from B. amyloliquefaciens, we successfully obtained crystals of the YknX protein that diffracted X-rays to a resolution of 4.4 Å. We calculated an experimentally phased map using single-wavelength anomalous diffraction (SAD), revealing that YknX forms a hexameric assembly similar to that of MacA from Gram-negative bacteria. The hexameric assembly of YknX exhibited a funnel-like structure with a central channel and a conical mouth. Functional studies in vitro suggest that YknX can bind directly to peptidoglycan. Our study provides an improved understanding of the assembly of the YknWXYZ efflux pump and the role of YknX in the complex.
[Mh] Termos MeSH primário: Bacillus amyloliquefaciens/química
Proteínas de Bactérias/química
Proteínas de Bactérias/ultraestrutura
Proteínas de Fusão de Membrana/química
Proteínas de Fusão de Membrana/ultraestrutura
Peptidoglicano/química
[Mh] Termos MeSH secundário: Sítios de Ligação
Dimerização
Proteínas de Membrana Transportadoras
Modelos Químicos
Modelos Moleculares
Ligação Proteica
Conformação Proteica
Esporos Bacterianos/química
Esporos Bacterianos/ultraestrutura
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Membrane Fusion Proteins); 0 (Membrane Transport Proteins); 0 (Peptidoglycan)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170918
[St] Status:MEDLINE



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