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Pesquisa : A11.872.620.500.700 [Categoria DeCS]
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Ugrinowitsch, Carlos
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[PMID]:29324825
[Au] Autor:Damas F; Libardi CA; Ugrinowitsch C; Vechin FC; Lixandrão ME; Snijders T; Nederveen JP; Bacurau AV; Brum P; Tricoli V; Roschel H; Parise G; Phillips SM
[Ad] Endereço:School of Physical Education and Sport, University of São Paulo, São Paulo, São Paulo, Brazil.
[Ti] Título:Early- and later-phases satellite cell responses and myonuclear content with resistance training in young men.
[So] Source:PLoS One;13(1):e0191039, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Satellite cells (SC) are associated with skeletal muscle remodelling after muscle damage and/or extensive hypertrophy resulting from resistance training (RT). We recently reported that early increases in muscle protein synthesis (MPS) during RT appear to be directed toward muscle damage repair, but MPS contributes to hypertrophy with progressive muscle damage attenuation. However, modulations in acute-chronic SC content with RT during the initial (1st-wk: high damage), early (3rd-wk: attenuated damage), and later (10th-wk: no damage) stages is not well characterized. Ten young men (27 ± 1 y, 23.6 ± 1.0 kg·m-2) underwent 10-wks of RT and muscle biopsies (vastus-lateralis) were taken before (Pre) and post (48h) the 1st (T1), 5th (T2) and final (T3) RT sessions to evaluate fibre type specific SC content, cross-sectional area (fCSA) and myonuclear number by immunohistochemistry. We observed RT-induced hypertrophy after 10-wks of RT (fCSA increased ~16% in type II, P < 0.04; ~8% in type I [ns]). SC content increased 48h post-exercise at T1 (~69% in type I [P = 0.014]; ~42% in type II [ns]), and this increase was sustained throughout RT (pre T2: ~65%, ~92%; pre T3: ~30% [ns], ~87%, for the increase in type I and II, respectively, vs. pre T1 [P < 0.05]). Increased SC content was not coupled with changes in myonuclear number. SC have a more pronounced role in muscle repair during the initial phase of RT than muscle hypertrophy resulted from 10-wks RT in young men. Chronic elevated SC pool size with RT is important providing proper environment for future stresses or larger fCSA increases.
[Mh] Termos MeSH primário: Núcleo Celular/metabolismo
Proteínas Musculares/metabolismo
Células Satélites de Músculo Esquelético/fisiologia
Levantamento de Peso
[Mh] Termos MeSH secundário: Adulto
Seres Humanos
Masculino
Células Satélites de Músculo Esquelético/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Muscle Proteins)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191039


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[PMID]:28463680
[Au] Autor:Griger J; Schneider R; Lahmann I; Schöwel V; Keller C; Spuler S; Nazare M; Birchmeier C
[Ad] Endereço:Developmental Biology/Signal Transduction Group, Max Delbrück Center for Molecular Medicine (MDC) in the Helmholtz Society, Berlin, Germany.
[Ti] Título:Loss of Ptpn11 (Shp2) drives satellite cells into quiescence.
[So] Source:Elife;6, 2017 05 02.
[Is] ISSN:2050-084X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The equilibrium between proliferation and quiescence of myogenic progenitor and stem cells is tightly regulated to ensure appropriate skeletal muscle growth and repair. The non-receptor tyrosine phosphatase Ptpn11 (Shp2) is an important transducer of growth factor and cytokine signals. Here we combined complex genetic analyses, biochemical studies and pharmacological interference to demonstrate a central role of Ptpn11 in postnatal myogenesis of mice. Loss of Ptpn11 drove muscle stem cells out of the proliferative and into a resting state during muscle growth. This Ptpn11 function was observed in postnatal but not fetal myogenic stem cells. Furthermore, muscle repair was severely perturbed when Ptpn11 was ablated in stem cells due to a deficit in stem cell proliferation and survival. Our data demonstrate a molecular difference in the control of cell cycle withdrawal in fetal and postnatal myogenic stem cells, and assign to Ptpn11 signaling a key function in satellite cell activity.
[Mh] Termos MeSH primário: Proteína Tirosina Fosfatase não Receptora Tipo 11/metabolismo
Células Satélites de Músculo Esquelético/citologia
Células Satélites de Músculo Esquelético/metabolismo
[Mh] Termos MeSH secundário: Animais
Citocinas/metabolismo
Peptídeos e Proteínas de Sinalização Intercelular/metabolismo
Camundongos Endogâmicos C57BL
Proteína Tirosina Fosfatase não Receptora Tipo 11/genética
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cytokines); 0 (Intercellular Signaling Peptides and Proteins); EC 3.1.3.48 (Protein Tyrosine Phosphatase, Non-Receptor Type 11); EC 3.1.3.48 (Ptpn11 protein, mouse)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE


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[PMID]:28464938
[Au] Autor:Monge C; DiStasio N; Rossi T; Sébastien M; Sakai H; Kalman B; Boudou T; Tajbakhsh S; Marty I; Bigot A; Mouly V; Picart C
[Ad] Endereço:CNRS, UMR 5628, LMGP, 3 parvis Louis Néel, F-38016, Grenoble, France. claire.monge@ibcp.fr.
[Ti] Título:Quiescence of human muscle stem cells is favored by culture on natural biopolymeric films.
[So] Source:Stem Cell Res Ther;8(1):104, 2017 May 02.
[Is] ISSN:1757-6512
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Satellite cells are quiescent resident muscle stem cells that present an important potential to regenerate damaged tissue. However, this potential is diminished once they are removed from their niche environment in vivo, prohibiting the long-term study and genetic investigation of these cells. This study therefore aimed to provide a novel biomaterial platform for the in-vitro culture of human satellite cells that maintains their stem-like quiescent state, an important step for cell therapeutic studies. METHODS: Human muscle satellite cells were isolated from two donors and cultured on soft biopolymeric films of controlled stiffness. Cell adhesive phenotype, maintenance of satellite cell quiescence and capacity for gene manipulation were investigated using FACS, western blotting, fluorescence microscopy and electron microscopy. RESULTS: About 85% of satellite cells cultured in vitro on soft biopolymer films for 3 days maintained expression of the quiescence marker Pax7, as compared with 60% on stiffer films and 50% on tissue culture plastic. The soft biopolymeric films allowed satellite cell culture for up to 6 days without renewing the media. These cells retained their stem-like properties, as evidenced by the expression of stem cell markers and reduced expression of differentiated markers. In addition, 95% of cells grown on these soft biopolymeric films were in the G0/G1 stage of the cell cycle, as opposed to those grown on plastic that became activated and began to proliferate and differentiate. CONCLUSIONS: Our study identifies a new biomaterial made of a biopolymer thin film for the maintenance of the quiescence state of muscle satellite cells. These cells could be activated at any point simply by replating them onto a plastic culture dish. Furthermore, these cells could be genetically manipulated by viral transduction, showing that this biomaterial may be further used for therapeutic strategies.
[Mh] Termos MeSH primário: Células-Tronco Adultas/citologia
Proliferação Celular
Cultura Primária de Células/métodos
Células Satélites de Músculo Esquelético/citologia
[Mh] Termos MeSH secundário: Células-Tronco Adultas/efeitos dos fármacos
Células-Tronco Adultas/fisiologia
Biopolímeros/farmacologia
Diferenciação Celular
Células Cultivadas
Meios de Cultura/química
Seres Humanos
Masculino
Meia-Idade
Células Satélites de Músculo Esquelético/efeitos dos fármacos
Células Satélites de Músculo Esquelético/fisiologia
Tecidos Suporte/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biopolymers); 0 (Culture Media)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1186/s13287-017-0556-8


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[PMID]:28470519
[Au] Autor:Smolina N; Bruton J; Kostareva A; Sejersen T
[Ad] Endereço:Karolinska Institutet, Stockholm, Sweden. natalia.smolina@ki.se.
[Ti] Título:Assaying Mitochondrial Respiration as an Indicator of Cellular Metabolism and Fitness.
[So] Source:Methods Mol Biol;1601:79-87, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mitochondrial respiration is the most important generator of cellular energy under most circumstances. It is a process of energy conversion of substrates into ATP. The Seahorse equipment allows measuring oxygen consumption rate (OCR) in living cells and estimates key parameters of mitochondrial respiration in real-time mode. Through use of mitochondrial inhibitors, four key mitochondrial respiration parameters can be measured: basal, ATP production-linked, maximal, and proton leak-linked OCR. This approach requires application of mitochondrial inhibitors-oligomycin to block ATP synthase, FCCP-to make the inner mitochondrial membrane permeable for protons and allow maximum electron flux through the electron transport chain, and rotenone and antimycin A-to inhibit complexes I and III, respectively. This chapter describes the protocol of OCR assessment in the culture of primary myotubes obtained upon satellite cell fusion.
[Mh] Termos MeSH primário: Trifosfato de Adenosina/metabolismo
Bioensaio/instrumentação
Mitocôndrias/metabolismo
Fosforilação Oxidativa
Consumo de Oxigênio
[Mh] Termos MeSH secundário: Animais
Antimicina A/farmacologia
Carbonil Cianeto p-Trifluormetoxifenil Hidrazona/farmacologia
Respiração Celular
Sobrevivência Celular
Complexo I de Transporte de Elétrons/antagonistas & inibidores
Complexo III da Cadeia de Transporte de Elétrons/antagonistas & inibidores
Camundongos
Mitocôndrias/efeitos dos fármacos
Fibras Musculares Esqueléticas/efeitos dos fármacos
Fibras Musculares Esqueléticas/metabolismo
Oligomicinas/farmacologia
Cultura Primária de Células
Ionóforos de Próton/farmacologia
Rotenona/farmacologia
Células Satélites de Músculo Esquelético/efeitos dos fármacos
Células Satélites de Músculo Esquelético/metabolismo
Desacopladores/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Oligomycins); 0 (Proton Ionophores); 0 (Uncoupling Agents); 03L9OT429T (Rotenone); 370-86-5 (Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone); 642-15-9 (Antimycin A); 8L70Q75FXE (Adenosine Triphosphate); EC 1.10.2.2 (Electron Transport Complex III); EC 1.6.5.3 (Electron Transport Complex I)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-4939-6960-9_7


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[PMID]:29371665
[Au] Autor:Tosic M; Allen A; Willmann D; Lepper C; Kim J; Duteil D; Schüle R
[Ad] Endereço:Urologische Klinik und Zentrale Klinische Forschung, Universitätsklinikum Freiburg, Medizinische Fakultät, Albert-Ludwigs-University Freiburg, Breisacherstrasse 66, 79106, Freiburg, Germany.
[Ti] Título:Lsd1 regulates skeletal muscle regeneration and directs the fate of satellite cells.
[So] Source:Nat Commun;9(1):366, 2018 01 25.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Satellite cells are muscle stem cells required for muscle regeneration upon damage. Of note, satellite cells are bipotent and have the capacity to differentiate not only into skeletal myocytes, but also into brown adipocytes. Epigenetic mechanisms regulating fate decision and differentiation of satellite cells during muscle regeneration are not yet fully understood. Here, we show that elevated levels of lysine-specific demethylase 1 (Kdm1a, also known as Lsd1) have a beneficial effect on muscle regeneration and recovery after injury, since Lsd1 directly regulates key myogenic transcription factor genes. Importantly, selective Lsd1 ablation or inhibition in Pax7-positive satellite cells, not only delays muscle regeneration, but changes cell fate towards brown adipocytes. Lsd1 prevents brown adipocyte differentiation of satellite cells by repressing expression of the novel pro-adipogenic transcription factor Glis1. Together, downregulation of Glis1 and upregulation of the muscle-specific transcription program ensure physiological muscle regeneration.
[Mh] Termos MeSH primário: Adipócitos Marrons/metabolismo
Proteínas de Ligação a DNA/genética
Histona Desmetilases/genética
Fibras Musculares Esqueléticas/metabolismo
Regeneração/genética
Células Satélites de Músculo Esquelético/metabolismo
Fatores de Transcrição/genética
[Mh] Termos MeSH secundário: Adipócitos Marrons/citologia
Animais
Diferenciação Celular
Linhagem Celular
Proliferação Celular
Proteínas de Ligação a DNA/metabolismo
Epigênese Genética
Histona Desmetilases/metabolismo
Camundongos
Camundongos Endogâmicos C57BL
Desenvolvimento Muscular/genética
Fibras Musculares Esqueléticas/citologia
Músculo Esquelético/lesões
Músculo Esquelético/metabolismo
Fator de Transcrição PAX7/genética
Fator de Transcrição PAX7/metabolismo
Cultura Primária de Células
Células Satélites de Músculo Esquelético/citologia
Transdução de Sinais
Fatores de Transcrição/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA-Binding Proteins); 0 (Glis1 protein, mouse); 0 (PAX7 Transcription Factor); 0 (Pax7 protein, mouse); 0 (Transcription Factors); EC 1.14.11.- (Aof2 protein, mouse); EC 1.14.11.- (Histone Demethylases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180127
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02740-5


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[PMID]:29224337
[Au] Autor:Zhang L; Gong H; Sun Q; Zhao R; Jia Y
[Ad] Endereço:Key Laboratory of Animal Physiology & Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University , Nanjing 210095, P. R. China.
[Ti] Título:Spermidine-Activated Satellite Cells Are Associated with Hypoacetylation in ACVR2B and Smad3 Binding to Myogenic Genes in Mice.
[So] Source:J Agric Food Chem;66(2):540-550, 2018 Jan 17.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Spermidine is an acetyltransferase inhibitor and a specific inducer of autophagy. Recently, spermidine is identified as a potential therapeutic agent for age-related muscle atrophy and inherited myopathies. However, the effect of spermidine on nonpathological skeletal muscle remains unclear. In this study, long-term spermidine administration in mice lowered the mean cross-sectional area of the gastrocnemius muscle and reduced the expression of myosin heavy chain isoforms in the muscle, which was associated with ubiquitination. Moreover, spermidine supplementation induced autophagy in satellite cells and enhanced satellite cell proliferation. ChIP assay revealed that spermidine repressed H3K56ac in the promoter of ACVR2B and lowered the binding affinity of Smad3 to the promoters of Myf5 and MyoD. Altogether, our results indicate that long-term administration of spermidine can activate satellite cells, as well as enhance autophagy, eventually resulting in muscle atrophy. In addition, H3K56ac and Smad3 emerged as key determinants of satellite cell activation.
[Mh] Termos MeSH primário: Receptores de Activinas Tipo II/metabolismo
Células Satélites de Músculo Esquelético/metabolismo
Proteína Smad3/metabolismo
Espermidina/farmacologia
[Mh] Termos MeSH secundário: Acetilação
Receptores de Activinas Tipo II/genética
Animais
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Músculo Esquelético/metabolismo
Ligação Proteica/efeitos dos fármacos
Células Satélites de Músculo Esquelético/efeitos dos fármacos
Proteína Smad3/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Smad3 Protein); 0 (Smad3 protein, mouse); EC 2.7.11.30 (Activin Receptors, Type II); EC 2.7.11.30 (activin receptor type II-B); U87FK77H25 (Spermidine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171212
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04482


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[PMID]:28893664
[Au] Autor:Cho DS; Doles JD
[Ad] Endereço:Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905, USA. Electronic address: cho.dong@mayo.edu.
[Ti] Título:Single cell transcriptome analysis of muscle satellite cells reveals widespread transcriptional heterogeneity.
[So] Source:Gene;636:54-63, 2017 Dec 15.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Tissue specific stem cells are indispensable contributors to adult tissue maintenance, repair, and regeneration. In skeletal muscle, satellite cells (SCs) are the resident muscle stem cell population and are required to maintain skeletal muscle homeostasis throughout life. Increasing evidence suggests that SCs are a heterogeneous cell population with substantial biochemical and functional diversity. A major limitation in the field is an incomplete understanding of the nature and extent of this cellular heterogeneity. Single cell analyses are well suited to addressing this issue, especially when coupled to unbiased profiling paradigms such as high throughout RNA sequencing. We performed single cell RNA sequencing (scRNA-seq) on freshly isolated muscle satellite cells and found a surprising degree of heterogeneity at multiple levels, from muscle-specific transcripts to the broader SC transcriptome. We leveraged several comparative bioinformatics techniques and found that individual SCs enrich for unique transcript clusters. We propose that these gene expression "fingerprints" may contribute to observed functional SC diversity. Overall, these studies underscore the importance of several established SC signaling pathways/processes on a single cell level, implicate novel regulators of SC heterogeneity, and lay the groundwork for further investigation into SC heterogeneity in health and disease.
[Mh] Termos MeSH primário: Células Satélites de Músculo Esquelético/metabolismo
Transcriptoma
[Mh] Termos MeSH secundário: Animais
Perfilação da Expressão Gênica
Camundongos
Análise de Célula Única
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170913
[St] Status:MEDLINE


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[PMID]:28790171
[Au] Autor:Kamizaki K; Doi R; Hayashi M; Saji T; Kanagawa M; Toda T; Fukada SI; Ho HH; Greenberg ME; Endo M; Minami Y
[Ad] Endereço:From the Division of Cell Physiology, Department of Physiology and Cell Biology, and.
[Ti] Título:The Ror1 receptor tyrosine kinase plays a critical role in regulating satellite cell proliferation during regeneration of injured muscle.
[So] Source:J Biol Chem;292(38):15939-15951, 2017 Sep 22.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Ror family receptor tyrosine kinases, Ror1 and Ror2, play important roles in regulating developmental morphogenesis and tissue- and organogenesis, but their roles in tissue regeneration in adult animals remain largely unknown. In this study, we examined the expression and function of Ror1 and Ror2 during skeletal muscle regeneration. Using an skeletal muscle injury model, we show that expression of Ror1 and Ror2 in skeletal muscles is induced transiently by the inflammatory cytokines, TNF-α and IL-1ß, after injury and that inhibition of TNF-α and IL-1ß by neutralizing antibodies suppresses expression of and in injured muscles. Importantly, expression of , but not , was induced primarily in Pax7-positive satellite cells (SCs) after muscle injury, and administration of neutralizing antibodies decreased the proportion of Pax7-positive proliferative SCs after muscle injury. We also found that stimulation of a mouse myogenic cell line, C2C12 cells, with TNF-α or IL-1ß induced expression of Ror1 via NF-κB activation and that suppressed expression of Ror1 inhibited their proliferative responses in SCs. Intriguingly, SC-specific depletion of decreased the number of Pax7-positive SCs after muscle injury. Collectively, these findings indicate for the first time that Ror1 has a critical role in regulating SC proliferation during skeletal muscle regeneration. We conclude that Ror1 might be a suitable target in the development of diagnostic and therapeutic approaches to manage muscular disorders.
[Mh] Termos MeSH primário: Músculo Esquelético/lesões
Músculo Esquelético/fisiologia
Receptores Órfãos Semelhantes a Receptor Tirosina Quinase/metabolismo
Regeneração
Células Satélites de Músculo Esquelético/citologia
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Proliferação Celular
Regulação Enzimológica da Expressão Gênica
Interleucina-1beta/metabolismo
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Desenvolvimento Muscular
NF-kappa B/metabolismo
Fator de Transcrição PAX7/metabolismo
Receptores Órfãos Semelhantes a Receptor Tirosina Quinase/genética
Células Satélites de Músculo Esquelético/metabolismo
Transdução de Sinais
Fator de Necrose Tumoral alfa/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Interleukin-1beta); 0 (NF-kappa B); 0 (PAX7 Transcription Factor); 0 (Pax7 protein, mouse); 0 (Tumor Necrosis Factor-alpha); EC 2.7.10.1 (Receptor Tyrosine Kinase-like Orphan Receptors); EC 2.7.10.1 (Ror1 protein, mouse); EC 2.7.10.1 (Ror2 protein, mouse)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171009
[Lr] Data última revisão:
171009
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170810
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.785709


  9 / 1563 MEDLINE  
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[PMID]:28780350
[Au] Autor:Liu D; Xu JH; Tong HL; Li SF; Yan YQ
[Ad] Endereço:The Laboratory of Cell and Development, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
[Ti] Título:Effect of ELOVL3 expression on bovine skeletal muscle-derived satellite cell differentiation.
[So] Source:Biochem Biophys Res Commun;493(4):1457-1463, 2017 Dec 02.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:ELOVL3 is involved in elongating saturated and monounsaturated fatty acids, and is a critical enzyme for lipid accumulation in brown adipocytes during the early phase of tissue recruitment. In addition, ELOVL3 is related to increased fatty acid oxidation in brown adipocytes. However, the potential functions of ELOVL3 in bovine cells remain unclear. Herein, we aimed to elucidate the effect of the ELOVL3 on the differentiation of bovine skeletal muscle-derived satellite cells (MDSCs). Western blot and immunofluorescence analyses were used for elucidating ELOVL3 expression pattern in bovine MDSCs during differentiation in vitro. We activated or inhibited ELOVL3 to study the effect of alterations in its expression on in vitro differentiation of bovine MDSCs. ELOVL3 expression increased gradually during bovine MDSC differentiation, and its levels were higher in the more highly differentiated myotubes. Activation of ELOVL3 promoted MDSC differentiation, while inhibition of ELOVL3 hindered differentiation of these cells. Here, for the first time, we demonstrate the importance of ELOVL3 during bovine MDSC differentiation, which may assist in increasing beef cattle muscularity.
[Mh] Termos MeSH primário: Acetiltransferases/metabolismo
Células Satélites de Músculo Esquelético/citologia
Células Satélites de Músculo Esquelético/metabolismo
[Mh] Termos MeSH secundário: Acetiltransferases/antagonistas & inibidores
Acetiltransferases/genética
Animais
Sistemas CRISPR-Cas
Bovinos
Diferenciação Celular/genética
Diferenciação Celular/fisiologia
Células Cultivadas
Ativação Enzimática
Inibidores Enzimáticos/farmacologia
Regulação da Expressão Gênica no Desenvolvimento
Desenvolvimento Muscular/genética
Desenvolvimento Muscular/fisiologia
Interferência de RNA
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
RNA Interferente Pequeno/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Enzyme Inhibitors); 0 (RNA, Messenger); 0 (RNA, Small Interfering); EC 2.3.1.- (Acetyltransferases); EC 2.3.1.- (fatty acid elongases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170807
[St] Status:MEDLINE


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[PMID]:28757453
[Au] Autor:Jin CF; Li Y; Ding XB; Li X; Zhang LL; Liu XF; Guo H
[Ad] Endereço:College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China.
[Ti] Título:lnc133b, a novel, long non-coding RNA, regulates bovine skeletal muscle satellite cell proliferation and differentiation by mediating miR-133b.
[So] Source:Gene;630:35-43, 2017 Sep 30.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The proliferation and differentiation of skeletal muscle satellite cells is regulated by multiple regulatory factors including non-coding RNAs. It has been reported that miR-133b regulates myogenesis. In this study, we detected a novel lncRNA, lnc133b, which is completely complemented by mature miR-133b, indicating that lnc133b may regulate the expression of miR-133b by "sponge" miR-133b. A luciferase report assay confirmed that lnc133b interacts with miR-133b in regions complemented by miR-133b. We successfully constructed lnc133b gain/loss-of-function cell models by infecting LV-1nc133b and transfecting si-lnc133b into satellite cells. Results of quantitative real-time polymerase chain reaction (qRT-PCR) and 5-ethynyl-2'-deoxyuridine (EdU) assays showed that overexpression or inhibition of lnc133b could promote the proliferation or inhibition of satellite cell differentiation. The qRT-PCR results also showed that lnc133b negatively regulates miR-133b expression and a Western blot assay showed that lnc133b positively regulates IGF1R expression, indicating that the lnc133b/miR-133b/IGF1R axis is a potential pathway for promoting satellite cell proliferation and repressing their differentiation through the ceRNA mechanism. Building on the findings of previous reports, we constructed the lnc133b/miR-133b/FGFR1 & PP2AC pathway to improve the lnc133b regulation network regulating the proliferation and differentiation of satellite cells. The current study provides a new perspective for understanding the mechanism regulating satellite cell proliferation and differentiation through the interaction of miR-133b and lnc133b.
[Mh] Termos MeSH primário: Diferenciação Celular
Proliferação Celular
MicroRNAs/genética
RNA Longo não Codificante/genética
Células Satélites de Músculo Esquelético/metabolismo
[Mh] Termos MeSH secundário: Animais
Bovinos
Células Cultivadas
Receptor IGF Tipo 1/genética
Receptor IGF Tipo 1/metabolismo
Células Satélites de Músculo Esquelético/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MicroRNAs); 0 (RNA, Long Noncoding); EC 2.7.10.1 (Receptor, IGF Type 1)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170801
[St] Status:MEDLINE



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