Base de dados : MEDLINE
Pesquisa : A11.872.785 [Categoria DeCS]
Referências encontradas : 303 [refinar]
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[PMID]:28441346
[Au] Autor:Yang R; Li P; Li N; Zhang Q; Bai X; Wang L; Xiao Y; Sun L; Yang Q; Yan J
[Ad] Endereço:Key Laboratory of Tropical Agro Environment, Ministry of Agriculture and Guangdong Engineering Research Centre for Modern Eco-Agriculture, South China Agricultural University, Guangzhou 510642, China. renyueyang123@126.com.
[Ti] Título:Xanthones from the Pericarp of Garcinia mangostana.
[So] Source:Molecules;22(5), 2017 Apr 25.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Mangosteen ( L.) is one of the most popular tropical fruits (called the "Queen of Fruits"), and is a rich source of oxygenated and prenylated xanthone derivatives. In the present work, phytochemical investigation has resulted in one new prenylated xanthone and 13 known xanthones isolated from the pericarp of . Their structures were established by spectroscopic data analysis, including X-ray diffraction. The new one was further tested for cytotoxic activity against seven cancer cell lines (CNE-1, CNE-2, A549, H490, PC-3, SGC-7901, U87), displaying the half maximal inhibitory concentration (IC50) values 3.35, 4.01, 4.84, 7.84, 6.21, 8.09, and 6.39 µM, respectively. It is noteworthy that the new compound can promote CNE-2 cells apoptosis in late stage, having a remarkable inhibition effect on the side population growth of CNE-2 at 1.26 µM. The bioactive compound was also detected in extract from fresh mangosteen flesh, which indicated that the popular fruit could have potential cytotoxic activity for cancer cell lines.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/química
Frutas/química
Garcinia mangostana/química
Extratos Vegetais/química
Xantonas/química
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/isolamento & purificação
Antineoplásicos Fitogênicos/farmacologia
Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
Ensaios de Seleção de Medicamentos Antitumorais
Seres Humanos
Concentração Inibidora 50
Células-Tronco Neoplásicas/efeitos dos fármacos
Células-Tronco Neoplásicas/fisiologia
Extratos Vegetais/isolamento & purificação
Extratos Vegetais/farmacologia
Células da Side Population
Xantonas/isolamento & purificação
Xantonas/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Plant Extracts); 0 (Xanthones)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170426
[St] Status:MEDLINE


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[PMID]:28397046
[Au] Autor:Hu J; Li J; Yue X; Wang JC; Wang JF; Liu JZ; Kong DL
[Ad] Endereço:Department of Colorectal Cancer Surgery, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy of Tianjin, Tianjin's Clinical Research Center for Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin, 300060, China.
[Ti] Título:Targeting BCRP/ABCG2 by RNA interference enhances the chemotherapy sensitivity of human colon cancer side population cells.
[So] Source:J Huazhong Univ Sci Technolog Med Sci;37(2):231-236, 2017 Apr.
[Is] ISSN:1672-0733
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:Relapse and metastasis are frequent in colon cancer and may be linked to stem cell characteristics. This study isolated side population (SP) cells from a colon cancer cell line (Colo-320) and examined their self-renewal and differentiation abilities. Compared to non-SP (NSP) cells, SP colon cancer cells were more tumorigenic in vivo and exhibited more invasive characteristics and a greater ability to form colonies. Additionally, more cells were in G /G phase and more highly expressed the multidrug resistance protein BCRP/ABCG2. We achieved enhanced chemotherapy sensitivity by transfecting SP cells with a hairpin-like, small interfering RNA (siRNA) eukaryotic expression plasmid targeting BCRP/ABCG2.
[Mh] Termos MeSH primário: Membro 2 da Subfamília G de Transportadores de Cassetes de Ligação de ATP/antagonistas & inibidores
Neoplasias do Colo/terapia
Fluoruracila/administração & dosagem
Proteínas de Neoplasias/antagonistas & inibidores
Células-Tronco Neoplásicas/efeitos dos fármacos
RNA Interferente Pequeno/farmacologia
Células da Side Population/efeitos dos fármacos
[Mh] Termos MeSH secundário: Membro 2 da Subfamília G de Transportadores de Cassetes de Ligação de ATP/genética
Animais
Diferenciação Celular/efeitos dos fármacos
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Neoplasias do Colo/genética
Neoplasias do Colo/metabolismo
Sinergismo Farmacológico
Tratamento Farmacológico
Fluoruracila/farmacologia
Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos
Seres Humanos
Camundongos
Proteínas de Neoplasias/genética
Células-Tronco Neoplásicas/patologia
Células da Side Population/patologia
Ensaios Antitumorais Modelo de Xenoenxerto
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ABCG2 protein, human); 0 (ATP Binding Cassette Transporter, Sub-Family G, Member 2); 0 (Neoplasm Proteins); 0 (RNA, Small Interfering); U3P01618RT (Fluorouracil)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171104
[Lr] Data última revisão:
171104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170412
[St] Status:MEDLINE
[do] DOI:10.1007/s11596-017-1720-1


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[PMID]:28219421
[Au] Autor:Choi SY; Kim HR; Ryu PD; Lee SY
[Ad] Endereço:Laboratory of Veterinary Pharmacology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Korea.
[Ti] Título:Regulation of voltage-gated potassium channels attenuates resistance of side-population cells to gefitinib in the human lung cancer cell line NCI-H460.
[So] Source:BMC Pharmacol Toxicol;18(1):14, 2017 Feb 21.
[Is] ISSN:2050-6511
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Side-population (SP) cells that exclude anti-cancer drugs have been found in various tumor cell lines. Moreover, SP cells have a higher proliferative potential and drug resistance than main population cells (Non-SP cells). Also, several ion channels are responsible for the drug resistance and proliferation of SP cells in cancer. METHODS: To confirm the expression and function of voltage-gated potassium (Kv) channels of SP cells, these cells, as well as highly expressed ATP-binding cassette (ABC) transporters and stemness genes, were isolated from a gefitinib-resistant human lung adenocarcinoma cell line (NCI-H460), using Hoechst 33342 efflux. RESULTS: In the present study, we found that mRNA expression of Kv channels in SP cells was different compared to Non-SP cells, and the resistance of SP cells to gefitinib was weakened with a combination treatment of gefitinib and Kv channel blockers or a Kv7 opener, compared to single-treatment gefitinib, through inhibition of the Ras-Raf signaling pathway. CONCLUSIONS: The findings indicate that Kv channels in SP cells could be new targets for reducing the resistance to gefitinib.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Resistência a Medicamentos Antineoplásicos/fisiologia
Neoplasias Pulmonares/metabolismo
Canais de Potássio de Abertura Dependente da Tensão da Membrana/fisiologia
Quinazolinas/farmacologia
Células da Side Population/metabolismo
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
Sobrevivência Celular/fisiologia
Relação Dose-Resposta a Droga
Resistência a Medicamentos Antineoplásicos/efeitos dos fármacos
Seres Humanos
Células da Side Population/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Potassium Channels, Voltage-Gated); 0 (Quinazolines); S65743JHBS (gefitinib)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170222
[St] Status:MEDLINE
[do] DOI:10.1186/s40360-017-0118-9


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[PMID]:28019667
[Au] Autor:Abbaszadegan MR; Bagheri V; Razavi MS; Momtazi AA; Sahebkar A; Gholamin M
[Ad] Endereço:Human Genetic Division, Immunology Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
[Ti] Título:Isolation, identification, and characterization of cancer stem cells: A review.
[So] Source:J Cell Physiol;232(8):2008-2018, 2017 Aug.
[Is] ISSN:1097-4652
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cancer stem cells (CSCs) or tumor-initiating cells (TICs) as a small subset of neoplastic cells are able to produce a tumor (tumorigenesis), maintain the population of tumorigenic cells (self-renewal), and generate the heterogeneous cells constructing the entire tumor (pluripotency). The research on stationary and circulating CSCs due to resistance to conventional therapies and inability in complete eradication of cancer is critical for developing novel therapeutic strategies for a more effective reduction in the risk of tumor metastasis and cancer recurrence. This review compiles information about different methods of detection and dissociation, side population, cellular markers, and establishment culture of CSCs, as well as characteristics of CSCs such as tumorigenicity, and signaling pathways associated with self-renewal and the capability of the same histological tumor regeneration in various cancers.
[Mh] Termos MeSH primário: Separação Celular/métodos
Neoplasias/patologia
Células Neoplásicas Circulantes/patologia
Células-Tronco Neoplásicas/patologia
Células da Side Population/patologia
[Mh] Termos MeSH secundário: Animais
Antineoplásicos/uso terapêutico
Biomarcadores Tumorais/metabolismo
Movimento Celular
Autorrenovação Celular
Resistência a Medicamentos Antineoplásicos
Seres Humanos
Metástase Neoplásica
Neoplasias/tratamento farmacológico
Neoplasias/metabolismo
Células Neoplásicas Circulantes/efeitos dos fármacos
Células Neoplásicas Circulantes/metabolismo
Células-Tronco Neoplásicas/efeitos dos fármacos
Células-Tronco Neoplásicas/metabolismo
Fenótipo
Células da Side Population/efeitos dos fármacos
Células da Side Population/metabolismo
Transdução de Sinais
Células Tumorais Cultivadas
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Biomarkers, Tumor)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170807
[Lr] Data última revisão:
170807
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161227
[St] Status:MEDLINE
[do] DOI:10.1002/jcp.25759


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[PMID]:27847151
[Au] Autor:Wang XZ; Gao RL; Sun P; Liu S; Xu Y; Liang DZ; Yin LM; Phillips WD; Liang SX
[Ad] Endereço:Department of Clinical Laboratory, the First Affiliated Hospital of Jinzhou Medical University, Jinzhou 121001,China.
[Ti] Título:Proliferation, differentiation and migration of SCA1 /CD31 cardiac side population cells in vitro and in vivo.
[So] Source:Int J Cardiol;227:378-386, 2017 Jan 15.
[Is] ISSN:1874-1754
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Side-population (SP) cells, identified by their capacity to efflux Hoechst dye, are highly enriched for stem/progenitor cell activity. They are found in many mammalian tissues, including mouse heart. Studies suggest that cardiac SP (CSP) cells can be divided into SCA1 /CD31 , SCA1 /CD31 and SCA1 /CD31 CSP subpopulations. SCA1 /CD31 were shown to be cardiac and endothelial stem/progenitors while SCA1 /CD31 CSP cells are endothelial progenitors. SCA1 /CD31 CSP cells remain to be fully characterized. In this study, we characterized SCA1 /CD31 CSP cells in the adult mouse heart, and investigated their abilities to proliferate, differentiate and migrate in vitro and in vivo. METHODS AND RESULTS: Using fluorescence-activated cell sorting, reverse transcriptase/polymerase chain reaction, assays of cell proliferation, differentiation and migration, and a murine model of myocardial infarction we show that SCA1 /CD31 CSP cells are located in the heart mesenchyme and express genes characteristic of stem cells and endothelial progenitors. These cells were capable of proliferation, differentiation, migration and vascularization in vitro and in vivo. Following experimental myocardial infarction, the SCA1 /CD31 CSP cells migrated from non-infarcted areas to the infarcted region within the myocardium where they differentiated into endothelial cells forming vascular (tube-like) structures. We further demonstrated that the SDF-1α/CXCR4 pathway may play an important role in migration of these cells after myocardial infarction. CONCLUSIONS: Based on their gene expression profile, localization and ability to proliferate, differentiate, migrate and vascularize in vitro and in vivo, we conclude that SCA1 /CD31 CSP cells may serve as endothelial progenitor cells in the adult mouse heart.
[Mh] Termos MeSH primário: Ataxina-1/fisiologia
Células Endoteliais/fisiologia
Infarto do Miocárdio/patologia
Miócitos Cardíacos/fisiologia
Molécula-1 de Adesão Celular Endotelial de Plaquetas/fisiologia
Células da Side Population/fisiologia
[Mh] Termos MeSH secundário: Animais
Técnicas de Cultura de Células
Diferenciação Celular
Movimento Celular
Proliferação Celular
Modelos Animais de Doenças
Feminino
Camundongos
Camundongos Endogâmicos C57BL
Infarto do Miocárdio/etiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ataxin-1); 0 (Platelet Endothelial Cell Adhesion Molecule-1)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161117
[St] Status:MEDLINE


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[PMID]:25689105
[Au] Autor:de Sá Rodrigues LC; Holmes KE; Thompson V; Newton MA; Stein TJ
[Ad] Endereço:Department of Medical Sciences, University of Wisconsin-Madison, Madison, WI, USA.
[Ti] Título:Tumourigenic canine osteosarcoma cell lines associated with frizzled-6 up-regulation and enhanced side population cell frequency.
[So] Source:Vet Comp Oncol;15(1):78-93, 2017 Mar.
[Is] ISSN:1476-5829
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:An increased serum alkaline phosphatase concentration is known to be associated with a negative prognosis in canine and human osteosarcoma. To expand upon previous studies regarding the biological relevance of increased serum alkaline phosphatase as a negative prognostic factor, xenogeneic heterotopic transplants were performed using six canine primary osteosarcoma cell lines generated from patients with differing serum alkaline phosphatase concentrations (three normal and three increased). Three of the six cell lines were capable of generating tumours and tumour formation was independent of the serum alkaline phosphatase status of the cell line. Microarray analysis identified 379 genes as being differentially expressed between the tumourigenic and non-tumourigenic cell lines. Frizzled-6 was upregulated to the greatest extent (7.78-fold) in tumourigenic cell lines compared with non-tumourigenic cell lines. Frizzled-6, a co-receptor for Wnt ligands has been associated with enhanced tumour-initiating cells and poor prognosis for other tumours. The increased expression of frizzled-6 was confirmed by quantitative reverse transcription polymerase chain reaction (QPCR) and Western blot analysis. Additionally, the tumourigenic cell lines also had an increase in the percentage of side population cells compared with non-tumourigenic cell lines (5.89% versus 1.58%, respectively). There were no differences in tumourigenicity, frizzled-6 or percentage of side population cells noted between osteosarcoma cell lines generated from patients of differing serum alkaline phosphatase concentration. However, to our knowledge this is the first study to identified frizzled-6 as a possible marker of osteosarcoma cell populations with enhanced tumourigenicity and side population cells. Future work will focus on defining the role of frizzled-6 in osteosarcoma tumourigenesis and tumour-initiating cells.
[Mh] Termos MeSH primário: Neoplasias Ósseas/veterinária
Doenças do Cão/genética
Osteossarcoma/veterinária
[Mh] Termos MeSH secundário: Fosfatase Alcalina/metabolismo
Animais
Biomarcadores Tumorais/genética
Neoplasias Ósseas/genética
Neoplasias Ósseas/metabolismo
Linhagem Celular Tumoral
Doenças do Cão/metabolismo
Cães
Expressão Gênica
Camundongos
Camundongos Nus
Análise em Microsséries/veterinária
Osteossarcoma/genética
Osteossarcoma/metabolismo
Prognóstico
Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária
Células da Side Population
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor); EC 3.1.3.1 (Alkaline Phosphatase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170814
[Lr] Data última revisão:
170814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150218
[St] Status:MEDLINE
[do] DOI:10.1111/vco.12138


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[PMID]:27851764
[Au] Autor:Prasanphanich AF; White DE; Gran MA; Kemp ML
[Ad] Endereço:The Wallace H. Coulter Department of Biomedical Engineering Georgia Institute of Technology and Emory University, Atlanta, Georgia, United States of America.
[Ti] Título:Kinetic Modeling of ABCG2 Transporter Heterogeneity: A Quantitative, Single-Cell Analysis of the Side Population Assay.
[So] Source:PLoS Comput Biol;12(11):e1005188, 2016 Nov.
[Is] ISSN:1553-7358
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The side population (SP) assay, a technique used in cancer and stem cell research, assesses the activity of ABC transporters on Hoechst staining in the presence and absence of transporter inhibition, identifying SP and non-SP cell (NSP) subpopulations by differential staining intensity. The interpretation of the assay is complicated because the transporter-mediated mechanisms fail to account for cell-to-cell variability within a population or adequately control the direct role of transporter activity on staining intensity. We hypothesized that differences in dye kinetics at the single-cell level, such as ABCG2 transporter-mediated efflux and DNA binding, are responsible for the differential cell staining that demarcates SP/NSP identity. We report changes in A549 phenotype during time in culture and with TGFß treatment that correlate with SP size. Clonal expansion of individually sorted cells re-established both SP and NSPs, indicating that SP membership is dynamic. To assess the validity of a purely kinetics-based interpretation of SP/NSP identity, we developed a computational approach that simulated cell staining within a heterogeneous cell population; this exercise allowed for the direct inference of the role of transporter activity and inhibition on cell staining. Our simulated SP assay yielded appropriate SP responses for kinetic scenarios in which high transporter activity existed in a portion of the cells and little differential staining occurred in the majority of the population. With our approach for single-cell analysis, we observed SP and NSP cells at both ends of a transporter activity continuum, demonstrating that features of transporter activity as well as DNA content are determinants of SP/NSP identity.
[Mh] Termos MeSH primário: Membro 2 da Subfamília G de Transportadores de Cassetes de Ligação de ATP/metabolismo
Bioensaio/métodos
Citometria de Fluxo/métodos
Microscopia de Fluorescência/métodos
Proteínas de Neoplasias/metabolismo
Células da Side Population/citologia
Células da Side Population/metabolismo
[Mh] Termos MeSH secundário: Cinética
Taxa de Depuração Metabólica
Modelos Biológicos
Células da Side Population/classificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ABCG2 protein, human); 0 (ATP Binding Cassette Transporter, Sub-Family G, Member 2); 0 (Neoplasm Proteins)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170530
[Lr] Data última revisão:
170530
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161117
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pcbi.1005188


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[PMID]:27821288
[Au] Autor:Yan W; Du J; Du Y; Pu H; Liu S; He J; Zhang J; Hou J
[Ad] Endereço:Department of Hematology, The Myeloma & Lymphoma Center, Changzheng Hospital, The Second Military Medical University, Shanghai, China.
[Ti] Título:Fenretinide targets the side population in myeloma cell line NCI-H929 and potentiates the efficacy of antimyeloma with bortezomib and dexamethasone regimen.
[So] Source:Leuk Res;51:32-40, 2016 Dec.
[Is] ISSN:1873-5835
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Side population (SP) cells, a subset of enriched tumor initiating cells, have been demonstrated to have stem cell-like properties in multiple myeloma (MM) by us as well as other previous studies. A lack of agents targeting tumor initiating cells, however, represents a challenge in the treatment of MM. Previously, fenretinide, a well-tolerated vitamin A derivative, has been shown to exert effect on leukemic stem cells, but its actions against myeloma stem-like cells are still unknown. In this study, the effects of fenretinide on myeloma stem-like cells characteristic was comprehensively examined in SP and non-SP (MP) cells of NCI-H929 cell sorted by flow cytometry-based on Hoechst 33342 stain. We find that fenretinide is capable of eradicating MM SP and MP cells, but not normal bone marrow mononuclear cells (BMMCs) at physiologically achievable concentrations. Fenretinide alone exerted a selective cytotoxic effect on MM SP cells, as well as in combination with bortezomib and dexamethasone. In particular, SP cells were highly sensitive to fenretinide, and in combination with bortezomib and dexamethasone in colony formation and apoptosis assays. Accordingly, the apparent fenretinide-induced-apoptosis was linked to the rapid generation of reactive oxygen species (ROS). Therefore, we propose that fenretinide is a potent agent that targets tumor initiating cells and may be a promising therapeutic agent in MM treatment.
[Mh] Termos MeSH primário: Fenretinida/farmacologia
Mieloma Múltiplo/tratamento farmacológico
Células da Side Population/efeitos dos fármacos
[Mh] Termos MeSH secundário: Protocolos de Quimioterapia Combinada Antineoplásica/farmacologia
Apoptose
Bortezomib/farmacologia
Bortezomib/uso terapêutico
Linhagem Celular Tumoral
Dexametasona/farmacologia
Dexametasona/uso terapêutico
Fenretinida/uso terapêutico
Seres Humanos
Mieloma Múltiplo/patologia
Células-Tronco Neoplásicas/efeitos dos fármacos
Células-Tronco Neoplásicas/patologia
Espécies Reativas de Oxigênio
Células da Side Population/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Reactive Oxygen Species); 187EJ7QEXL (Fenretinide); 69G8BD63PP (Bortezomib); 7S5I7G3JQL (Dexamethasone)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170613
[Lr] Data última revisão:
170613
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161109
[St] Status:MEDLINE


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[PMID]:27814696
[Au] Autor:Murota Y; Tabu K; Taga T
[Ad] Endereço:Department of Stem Cell Regulation, Medical Research Institute, Tokyo Medical and Dental University (TMDU), Bunkyo-ku, Tokyo, 1138510, Japan.
[Ti] Título:Requirement of ABC transporter inhibition and Hoechst 33342 dye deprivation for the assessment of side population-defined C6 glioma stem cell metabolism using fluorescent probes.
[So] Source:BMC Cancer;16(1):847, 2016 11 04.
[Is] ISSN:1471-2407
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Elucidating the precise properties of cancer stem cells (CSCs) is indispensable for the development of effective therapies against tumors, because CSCs are key drivers of tumor development, metastasis and relapse. We previously reported that the Hoechst 33342 dye-low staining side population (SP) method can enrich for CSCs in the C6 glioma cell line, and that the positively stained main population (MP) cells are non-CSCs. Presence of cancer stem-like SP cells is reported in various types of cancer. Although altered cellular energy metabolism is a hallmark of cancer, very little has been studied on the applicability of fluorescent probes for the understanding of CSC energy metabolism. METHODS: The metabolic status of C6 SP and MP cells are evaluated by CellROX, MitoTracker Green (MTG) and JC-1 for cellular oxidative stress, mitochondrial amount, and mitochondrial membrane potential, respectively. RESULTS: SP cells were found to exhibit significantly lower fluorescent intensities of CellROX and MTG than MP cells. However, inhibition of ATP binding cassette (ABC) transporters by verapamil enhanced the intensities of these probes in SP cells to the levels similar to those in MP cells, indicating that SP cells expel the probes outside of the cells through ABC transporters. Next, SP cells were stained with JC-1 dye which exhibits membrane potential dependent accumulation in mitochondrial matrix, followed by formation of aggregates. The mitochondrial membrane potential indicated by the aggregates of JC-1 was 5.0-fold lower in SP cells than MP cells. Inhibition of ABC transporters enhanced the fluorescent intensities of the JC-1 aggregates in both SP and MP cells, the former of which was still 2.2-fold lower than the latter. This higher JC-1 signal in MP cells was further found to be due to the Hoechst 33342 dye existing in MP cells. When SP and MP cells were recultured to deprive the intracellular Hoechst 33342 dye and then stained with JC-1 in the presence of verapamil, the intensities of JC-1 aggregates in such SP and MP cells became comparable. CONCLUSION: Inhibiting ABC transporters and depriving Hoechst 33342 dye are required for the accurate assessment of side population-defined C6 glioma stem cell metabolism using fluorescent probes.
[Mh] Termos MeSH primário: Transportadores de Cassetes de Ligação de ATP/metabolismo
Glioma/metabolismo
Células-Tronco Neoplásicas/metabolismo
Células da Side Population/metabolismo
[Mh] Termos MeSH secundário: Transportadores de Cassetes de Ligação de ATP/antagonistas & inibidores
Animais
Biomarcadores
Linhagem Celular Tumoral
Corantes Fluorescentes
Glioma/patologia
Seres Humanos
Imunofenotipagem
Células-Tronco Neoplásicas/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biomarkers); 0 (Fluorescent Dyes)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171121
[Lr] Data última revisão:
171121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161106
[St] Status:MEDLINE


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[PMID]:27573045
[Au] Autor:Chen Y; Zhao J; Luo Y; Wang Y; Jiang Y
[Ad] Endereço:Department of Urology, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 100029, P.R. China.
[Ti] Título:Downregulated expression of miRNA-149 promotes apoptosis in side population cells sorted from the TSU prostate cancer cell line.
[So] Source:Oncol Rep;36(5):2587-2600, 2016 Nov.
[Is] ISSN:1791-2431
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:The objective of the present study was to identify prostate cancer stem cells and determine the effects of modulating specific miRNAs on prostate CSC proliferation and apoptosis. We applied flow cytometry sorting of side population cells to cultures of prostate cancer cell lines (TSU, DU145, PC-3 and LNCaP). The proportion of SP cells in the TSU line was 1.60±0.40% (mean ± SD), while that of the DU145, PC-3 and LNCaP lines was 0.60±0.05, 0.80±0.05 and 0.60±0.20%, respectively. Because the proportion of SP cells derived from TSU cells is greater, these cells were selected to sort side population cells and non-side population cells. The stem-like properties of SP cells had been identified by in vivo and in vitro experiments, and the related study was published. RNA was extracted from the SP cells and non-SP cells and analyzed using miRNA microarray technology. Fifty-three miRNAs with significant differences in their expression were detected in total. Furthermore, 20 of these miRNAs were validated by qPCR. We found that hsa-miR­149 expression in SP cells and non-SP cells was significantly different; hsa-miR-149 was significantly upregulated in SP cells. By constructing a vector for lentiviral infection, we found that the downregulation of hsa-miR-149 leads to a reduction in proliferation, an increase in apoptosis, and a significant reduction in the colony formation potential, thus, inhibiting tumor growth in vivo of SP cells from the TSU cell line. The present study will provide new avenues toward understanding the function of prostate cancer stem cells (PCSCs) in tumorigenicity and metastasis.
[Mh] Termos MeSH primário: Apoptose/genética
Proliferação Celular/genética
MicroRNAs/biossíntese
Neoplasias da Próstata/genética
[Mh] Termos MeSH secundário: Movimento Celular/genética
Citometria de Fluxo
Regulação Neoplásica da Expressão Gênica
Seres Humanos
Lentivirus/genética
Masculino
MicroRNAs/genética
Células-Tronco Neoplásicas/metabolismo
Células-Tronco Neoplásicas/patologia
Neoplasias da Próstata/patologia
Células da Side Population/metabolismo
Transfecção
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MIRN149 microRNA, human); 0 (MicroRNAs)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170310
[Lr] Data última revisão:
170310
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160831
[St] Status:MEDLINE
[do] DOI:10.3892/or.2016.5047



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