Base de dados : MEDLINE
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  1 / 17205 MEDLINE  
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[PMID]:29188665
[Au] Autor:Zou KN; Hu M; Huang JP; Zhou HG
[Ad] Endereço:Shanghai Key Laboratory of Crime Scene Evidence, Key Laboratory of Forensic Evidence and Science Technology, Ministry of Public Security, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China.
[Ti] Título:[Identification of Vaginal Fluid Using Microbial Signatures].
[So] Source:Fa Yi Xue Za Zhi;32(4):254-256, 2016 Aug.
[Is] ISSN:1004-5619
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVES: To investigate the specific microbial signatures in vaginal fluid. METHODS: Vaginal fluid (16 samples), saliva (16 samples), feces (16 samples), semen (8 samples), peripheral blood (8 samples), urine (5 samples), and nasal secretion (4 samples) were collected respectively. The genes of , , , , and were amplified. PCR production was detected via a 3130xl Genetic Analyzer. RESULTS: The detected number of , , , , and were 15, 5, 8, 14, and 3 in all vaginal fluid samples, respectively. and existed specifically in vaginal fluid. CONCLUSIONS: There is a potential application value to detect and for the identification of vaginal fluid.
[Mh] Termos MeSH primário: Líquidos Corporais/microbiologia
Vagina/microbiologia
[Mh] Termos MeSH secundário: Actinobacteria/classificação
Sangue/microbiologia
Fezes/microbiologia
Feminino
Genes Bacterianos
Seres Humanos
Lactobacillus/classificação
Cavidade Nasal/microbiologia
Reação em Cadeia da Polimerase
RNA Ribossômico 16S/genética
Saliva/microbiologia
Sêmen/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171201
[St] Status:MEDLINE
[do] DOI:10.3969/j.issn.1004-5619.2016.04.004


  2 / 17205 MEDLINE  
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[PMID]:29421440
[Au] Autor:Wen A; Qu XH; Zhang KN; Leng EL; Ren Y; Wu XM
[Ad] Endereço:Department of Neurology, People's Hospital of Jiangxi Province, Nanchang, Jiangxi Province, China; Institution of Neurology, People's Hospital of Jiangxi Province, Nanchang, Jiangxi Province, China.
[Ti] Título:Evaluation of interferon-gamma release assays in extrasanguinous body fluids for diagnosing tuberculosis: A systematic review and meta-analysis.
[So] Source:Life Sci;197:140-146, 2018 Mar 15.
[Is] ISSN:1879-0631
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:AIMS: In this study, we conducted a meta-analysis to systematically compare the diagnostic accuracy of IGRAs performed for extrasanguinous body fluids with that performed for blood in the diagnosis of TB. MAIN METHODS: Multiple English and Chinese databases were searched up to November 2017. Studies that complied with the guidelines for the Quality Assessment of Diagnostic Accuracy Studies and used QuantiFERON-TB Gold In-Tube and/or T-SPOT.TB (ELISPOT) assays on both blood and extrasanguinous body fluids were included. Statistical analysis was performed using Stata 12.0 software. Since publication bias is a concern in the meta-analysis of diagnostic studies, we tested for this using Begg's funnel plots. KEY FINDING: Among the 1332 articles searched from the databases, 24 articles met the inclusion criteria, which included 1040 samples in the patient group and 1044 samples in the control group. For extrasanguinous body fluids, the pooled sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR), and summary receiver operating characteristic (SROC) area under the curve (AUC) were 87% (95% CI: 0.81-0.91), 89% (95% CI: 0.84-0.93), 8.22 (95% CI 5.38-12.56), 0.15 (95% CI: 0.10-0.21), 44.92 (95% CI: 25.61-78.81), and 0.94 (95% CI: 0.92-0.96), respectively. For peripheral blood, these values were 83% (95% CI: 0.79-0.87), 74% (95% CI: 0.68-0.79), 3.17 (95% CI 2.63-3.84), 0.23 (95% CI: 0.19-0.29), 12.99 (95% CI: 10.19-16.57), and 0.86 (95% CI: 0.82-0.89), respectively. SIGNIFICANCE: IGRAs performed on extrasanguinous body fluids exhibited a better diagnostic accuracy compared with IGRAs performed on peripheral blood for diagnosing TB.
[Mh] Termos MeSH primário: Líquidos Corporais/metabolismo
Interferon gama/metabolismo
Tuberculose/diagnóstico
Tuberculose/metabolismo
[Mh] Termos MeSH secundário: Seres Humanos
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; META-ANALYSIS; REVIEW
[Nm] Nome de substância:
0 (IFNG protein, human); 82115-62-6 (Interferon-gamma)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180209
[St] Status:MEDLINE


  3 / 17205 MEDLINE  
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[PMID]:29188671
[Au] Autor:Ding Y; Lu Q; Hu Y
[Ad] Endereço:Institute of Criminal Science and Technology, Pudong Branch of Shanghai Public Security Bureau, Shanghai 220135, China.
[Ti] Título:[Application of Postmortem Biochemistry in Forensic Diagnosis of Diabetic Ketoacidosis].
[So] Source:Fa Yi Xue Za Zhi;32(4):273-276, 2016 Aug.
[Is] ISSN:1004-5619
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:Diabetic ketoacidosis is a serious complication results from the high blood levels of glucose and ketone in diabetes mellitus patients that cause metabolic overbalance. An analysis of postmortem biochemical indexes is needed in such cases without specific signs of the routine forensic medicine examination. Postmortem biochemistry is a kind of examinations that collecting the body fluids of the corpses to determine the metabolic state of their life time to estimate the cause of death. This paper reviews the basic features and signs of the forensic medicine examination in the dead cases of diabetic ketoacidosis, and emphatically analyzes the postmortem biochemical indexes of diabetic ketoacidosis, and summarizes new ideas of forensic medicine diagnosis in diabetic ketoacidosis death.
[Mh] Termos MeSH primário: Líquidos Corporais/química
Cetoacidose Diabética/diagnóstico
Medicina Legal
[Mh] Termos MeSH secundário: Autopsia
Patologia Legal
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171201
[St] Status:MEDLINE
[do] DOI:10.3969/j.issn.1004-5619.2016.04.010


  4 / 17205 MEDLINE  
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[PMID]:28449921
[Au] Autor:Nwaiwu CA; Egro FM; Smith S; Harper JD; Spiess AM
[Ad] Endereço:University of Pittsburgh School of Medicine, Pittsburgh, PA.
[Ti] Título:Seroconversion rate among health care workers exposed to HIV-contaminated body fluids: The University of Pittsburgh 13-year experience.
[So] Source:Am J Infect Control;45(8):896-900, 2017 Aug 01.
[Is] ISSN:1527-3296
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The studies enumerating the risk of HIV transmission to health care workers (HCWs) as 0.3% after percutaneous exposure to HIV-positive blood, and 0.09% after a mucous membrane exposure, are weakened by dated literature. Our study aims to demonstrate the seroconversion rate after exposure to HIV-contaminated body fluids in a major academic center in the United States. METHODS: A prospectively maintained database of reported occupational injuries occurring between 2002 and 2015 at an academic medical center was analyzed. Data collected included the type of injury, injured body part, type of fluid, contamination of sharps, involvement of resident physicians, use of postexposure prophylaxis, and patients' HIV, hepatitis B virus, and hepatitis C virus status. RESULTS: A total of 266 cases were included in the study. Most exposures were caused by percutaneous injuries (52.6%), followed by 43.2% mucocutaneous injuries. Of the injuries, 52.6% were to the hand and 33.5% to the face and neck. Blood exposure accounted for 64.3% of all cases. Of the patients, 21.1% received postexposure prophylaxis. None of the HCWs exposed to HIV-contaminated body fluids seroconverted (seroconversion rate, 0%). CONCLUSIONS: HIV does not seem to be as easily transmitted by needlestick, laceration, or splash injuries as previously surmised. Further large-scale and multicenter studies are needed for a more accurate estimation of the risk of transmission of HIV in U.S. health care workers.
[Mh] Termos MeSH primário: Fármacos Anti-HIV/administração & dosagem
Líquidos Corporais/virologia
Infecções por HIV/transmissão
Soropositividade para HIV/epidemiologia
Profilaxia Pós-Exposição/métodos
[Mh] Termos MeSH secundário: Fármacos Anti-HIV/uso terapêutico
Pessoal de Saúde
Hospitais Universitários
Seres Humanos
Transmissão de Doença Infecciosa do Paciente para o Profissional
Exposição Ocupacional
Pennsylvania
Estudos Retrospectivos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-HIV Agents)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE


  5 / 17205 MEDLINE  
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[PMID]:29261742
[Au] Autor:Passannanti F; Nigro F; Gallo M; Tornatore F; Frasso A; Saccone G; Budelli A; Barone MV; Nigro R
[Ad] Endereço:DICMAPI, University of Naples Federico II, Naples, Italy.
[Ti] Título:In vitro dynamic model simulating the digestive tract of 6-month-old infants.
[So] Source:PLoS One;12(12):e0189807, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: In vivo assays cannot always be conducted because of ethical reasons, technical constraints or costs, but a better understanding of the digestive process, especially in infants, could be of great help in preventing food-related pathologies and in developing new formulas with health benefits. In this context, in vitro dynamic systems to simulate human digestion and, in particular, infant digestion could become increasingly valuable. OBJECTIVE: To simulate the digestive process through the use of a dynamic model of the infant gastroenteric apparatus to study the digestibility of starch-based infant foods. DESIGN: Using M.I.D.A (Model of an Infant Digestive Apparatus), the oral, gastric and intestinal digestibility of two starch-based products were measured: 1) rice starch mixed with distilled water and treated using two different sterilization methods (the classical method with a holding temperature of 121°C for 37 min and the HTST method with a holding temperature of 137°C for 70 sec) and 2) a rice cream with (premium product) or without (basic product) an aliquot of rice flour fermented by Lactobacillus paracasei CBA L74. After the digestion the foods were analyzed for the starch concentration, the amount of D-glucose released and the percentage of hydrolyzed starch. RESULTS: An in vitro dynamic system, which was referred to as M.I.D.A., was obtained. Using this system, the starch digestion occurred only during the oral and intestinal phase, as expected. The D-glucose released during the intestinal phase was different between the classical and HTST methods (0.795 grams for the HTST versus 0.512 for the classical product). The same analysis was performed for the basic and premium products. In this case, the premium product had a significant difference in terms of the starch hydrolysis percentage during the entire process. CONCLUSIONS: The M.I.D.A. system was able to digest simple starches and a more complex food in the correct compartments. In this study, better digestibility of the premium product was revealed.
[Mh] Termos MeSH primário: Trato Gastrointestinal/metabolismo
Modelos Biológicos
[Mh] Termos MeSH secundário: Líquidos Corporais
Digestão
Eletrólitos/química
Fermentação
Glucose/metabolismo
Seres Humanos
Hidrólise
Lactente
Oryza/química
Amido/metabolismo
Esterilização
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Electrolytes); 9005-25-8 (Starch); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189807


  6 / 17205 MEDLINE  
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[PMID]:29244819
[Au] Autor:Hinson KR; Reukov V; Benson EP; Zungoli PA; Bridges WC; Ellis BR; Song J
[Ad] Endereço:Department of Plant and Environmental Sciences, Clemson University Clemson, South Carolina, United States of America.
[Ti] Título:Climbing ability of teneral and sclerotized adult bed bugs and assessment of adhesive properties of the exoskeletal fluid using atomic force microscopy.
[So] Source:PLoS One;12(12):e0189215, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We observed that teneral adults (<1 h post-molt) of Cimex lectularius L. appeared more adept at climbing a smooth surface compared to sclerotized adults. Differences in climbing ability on a smooth surface based on sclerotization status were quantified by measuring the height to which bed bugs climbed when confined within a glass vial. The average maximum height climbed by teneral (T) bed bugs (n = 30, height climbed = 4.69 cm) differed significantly (P< 0.01) from recently sclerotized (RS) bed bugs (n = 30, height climbed = 1.73 cm at ~48 h post molt), sclerotized group 1 (S1) bed bugs (n = 30, S1 = 2.42 cm at >72 h), and sclerotized group 2 (S2) bed bugs (n = 30, height climbed = 2.64 cm at >72 h post molt). When heights from all climbing events were summed, teneral bed bugs (650.8 cm climbed) differed significantly (P< 0.01) from recently sclerotized (82 cm climbed) and sclerotized (group 1 = 104.6 cm climbed, group 2 = 107.8 cm climbed) bed bugs. These findings suggested that the external surface of teneral bed bug exoskeletons possess an adhesive property. Using atomic force microscopy (AFM), we found that adhesion force of an exoskeletal (presumably molting) fluid decreased almost five-fold from 88 to 17 nN within an hour of molting. Our findings may have implications for laboratory safety and the effectiveness of bed bug traps, barriers, and biomimetic-based adhesives.
[Mh] Termos MeSH primário: Percevejos-de-Cama/fisiologia
Líquidos Corporais/fisiologia
[Mh] Termos MeSH secundário: Animais
Locomoção
Microscopia de Força Atômica
Muda
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180105
[Lr] Data última revisão:
180105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189215


  7 / 17205 MEDLINE  
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[PMID]:29191359
[Au] Autor:Miller DL; Helms GA; Mayfield WR
[Ad] Endereço:WellStar Thoracic Surgery, WellStar Health System/Mayo Clinic Care Network, Marietta, Georgia. Electronic address: daniel.miller@wellstar.org.
[Ti] Título:Evaluation of Esophageal Anastomotic Integrity With Serial Pleural Amylase Levels.
[So] Source:Ann Thorac Surg;105(1):200-206, 2018 Jan.
[Is] ISSN:1552-6259
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: An anastomotic leak is the most devastating and potentially fatal complication after esophagectomy. Current detection methods can be inaccurate and place patients at risk of other complications. Analysis of pleural fluid for amylase may be more accurate and place patients at less of a risk for evaluating the integrity of an esophageal anastomosis. METHODS: We retrospectively reviewed prospective data of 45 consecutive patients who underwent an Ivor Lewis esophagectomy over an 18-month period and evaluated their anastomotic integrity with serial pleural amylase levels (PAL). RESULTS: There were 40 men (89%), and median age was 63 years (range, 35 to 79). Indication for esophagectomy was cancer in 38 patients (84%); 27 (71%) underwent neoadjuvant chemoradiation. A barium swallow was performed in the first 25 patients at median postoperative day (POD) 5 (range, 5 to 10); the swallow was negative in 23 patients (93%). Serial PALs were obtained starting on POD 3 and stopped 1 day after toleration of clear liquids. The PALs in the no-leak patients were highest on POD 3 (median 42 IU/L; range, 20 to 102 IU/L) and decreased (median 15 IU/L; range, 8 to 34 IU/L) to the lowest levels 1 day after clear liquid toleration (p = 0.04). Two patients had a leak and had peak PALs of 227 IU/L and 630 IU/L, respectively; both leaks occurred on POD 4, 1 day before their scheduled swallow test. The last 20 patients underwent serial PALs only, without a planned swallow test or computed tomography scan for anastomotic integrity evaluation. One of these patients had a leak on POD 5 with a low PAL of 55 IU/L the day before the spike of more than 4,000 IU/L. Two of the leaks were treated with esophageal stent placement and intravenous antibiotics, and the remaining patient's leak resolved with intravenous antibiotics, no oral intake, and observation only. None of the leak patients required transthoracic esophageal repair or drainage of an empyema. There was 1 postoperative death (2%) secondary to aspiration pneumonia on POD 10; no leak was ever identified, and the patient had been eating for 3 days before death. Complications occurred in 15 patients (33%), most commonly respiratory; no respiratory issues occurred in PAL-only evaluated patients. No late anastomotic leaks occurred in any patient while in the hospital or after discharge. CONCLUSIONS: Serial PALs for the detection of esophageal anastomotic leaks proved to be accurate, safe, and inexpensive. Elimination of barium swallows and computed tomography scans for evaluation of anastomotic integrity may decrease aspiration risks as well as associated pulmonary failure during the postoperative period. Serial PALs may be the preferred method of detecting an anastomotic leak after esophagectomy. A prospective randomized study is warranted.
[Mh] Termos MeSH primário: Amilases/análise
Fístula Anastomótica/diagnóstico
Líquidos Corporais/química
Esofagectomia
Esôfago/cirurgia
[Mh] Termos MeSH secundário: Adulto
Idoso
Anastomose Cirúrgica
Feminino
Seres Humanos
Masculino
Meia-Idade
Pleura
Estudos Retrospectivos
[Pt] Tipo de publicação:JOURNAL ARTICLE; OBSERVATIONAL STUDY
[Nm] Nome de substância:
EC 3.2.1.- (Amylases)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171228
[Lr] Data última revisão:
171228
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171202
[St] Status:MEDLINE


  8 / 17205 MEDLINE  
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[PMID]:29176840
[Au] Autor:Tran-Thi N; Lowe RJ; Schurer JM; Vu-Van T; MacDonald LE; Pham-Duc P
[Ad] Endereço:Center for Public Health and Ecosystem Research, Hanoi University of Public Health, North Tu Liem, Hanoi, Vietnam.
[Ti] Título:Turning poop into profit: Cost-effectiveness and soil transmitted helminth infection risk associated with human excreta reuse in Vietnam.
[So] Source:PLoS Negl Trop Dis;11(11):e0006088, 2017 Nov.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Human excreta is a low cost source of nutrients vital to plant growth, but also a source of pathogens transmissible to people and animals. We investigated the cost-savings and infection risk of soil transmitted helminths (STHs) in four scenarios where farmers used either inorganic fertilizer or fresh/composted human excreta supplemented by inorganic fertilizer to meet the nutrient requirements of rice paddies in the Red River Delta, Vietnam. Our study included two main components: 1) a risk estimate of STH infection for farmers who handle fresh excreta, determined by systematic review and meta-analysis; and 2) a cost estimate of fertilizing rice paddies, determined by nutrient assessment of excreta, a retailer survey of inorganic fertilizer costs, and a literature review to identify region-specific inputs. Our findings suggest that farmers who reuse fresh excreta are 1.24 (95% CI: 1.13-1.37, p-value<0.001) times more likely to be infected with any STH than those who do not handle excreta or who compost appropriately, and that risk varies by STH type (Ascaris lumbricoides RR = 1.17, 95% CI = 0.87-1.58, p-value = 0.29; Hookworm RR = 1.02, 95% CI = 0.50-2.06, p-value = 0.96; Trichuris trichiura RR = 1.38, 95% CI = 0.79-2.42, p-value = 0.26). Average cost-savings were highest for farmers using fresh excreta (847,000 VND) followed by those who composted for 6 months as recommended by the WHO (312,000 VND) and those who composted for a shorter time (5 months) with lime supplementation (37,000 VND/yr); however, this study did not assess healthcare costs of treating acute or chronic STH infections in the target group. Our study provides evidence that farmers in the Red River Delta are able to use a renewable and locally available resource to their economic advantage, while minimizing the risk of STH infection.
[Mh] Termos MeSH primário: Agricultura/métodos
Ascaríase/transmissão
Fezes/parasitologia
Infecções por Uncinaria/transmissão
Solo/parasitologia
Tricuríase/transmissão
[Mh] Termos MeSH secundário: Ancylostomatoidea/isolamento & purificação
Animais
Ascaríase/parasitologia
Ascaris lumbricoides/isolamento & purificação
Líquidos Corporais/parasitologia
Análise Custo-Benefício
Infecções por Uncinaria/parasitologia
Seres Humanos
Modelos Lineares
Tricuríase/parasitologia
Trichuris/isolamento & purificação
Vietnã
[Pt] Tipo de publicação:JOURNAL ARTICLE; META-ANALYSIS; REVIEW
[Nm] Nome de substância:
0 (Soil)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171219
[Lr] Data última revisão:
171219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0006088


  9 / 17205 MEDLINE  
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[PMID]:28990461
[Au] Autor:Oikawa R; Watanabe Y; Miyamoto S; Sato Y; Ono S; Mabe K; Yamamoto H; Kato M; Itoh F
[Ad] Endereço:1 Division of Gastroenterology and Hepatology, Department of Internal Medicine, St. Marianna University School of Medicine, Kawasaki, Japan.
[Ti] Título:Enrichment of Helicobacter pylori mutant strains after eradication therapy analyzed by gastric wash-based quantitative pyrosequencing.
[So] Source:Tumour Biol;39(10):1010428317734865, 2017 Oct.
[Is] ISSN:1423-0380
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The eradication of Helicobacter pylori reduces the risk of gastric cancer. A clear understanding of the factors underlying mixed infection with multiple clarithromycin-susceptible and clarithromycin-resistant H. pylori strains is necessary to design more effective therapies against H. pylori. We aimed to assess how the abundance and prevalence of H. pylori strains vary after clarithromycin-based eradication therapy. Using gastric wash samples, which represent the entire stomach, we sequentially analyzed the abundance and prevalence of H. pylori DNA by 23S ribosomal RNA pyrosequencing before and 1, 2, and 3 years after eradication therapy. Low levels of H. pylori DNA were still detectable at the first-year follow-up in all samples with negative post-treatment urea breath test results. The abundance of H. pylori DNA decreased significantly until the 2-year follow-up, but it switched to an increase at the 3-year follow-up. Importantly, the ratio of the prevalence of mutant strains to the prevalence of wild-type strains had already increased at the first-year follow-up and continued to increase, suggesting the selection and growth of clarithromycin-resistant strains during the follow-up periods. Being sensitive and representative, our assay will be useful in effectively addressing gastric cancer development by enhancing the long-term success of intervention strategies and consecutive surveillance for H. pylori eradication.
[Mh] Termos MeSH primário: Infecções por Helicobacter/microbiologia
Helicobacter pylori/genética
Neoplasias Gástricas/microbiologia
[Mh] Termos MeSH secundário: Idoso
Idoso de 80 Anos ou mais
Antibacterianos/uso terapêutico
Líquidos Corporais/microbiologia
Claritromicina/uso terapêutico
DNA Bacteriano/análise
Feminino
Seguimentos
Infecções por Helicobacter/tratamento farmacológico
Seres Humanos
Masculino
Meia-Idade
RNA Ribossômico 23S/análise
Reação em Cadeia da Polimerase em Tempo Real
Irrigação Terapêutica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (DNA, Bacterial); 0 (RNA, Ribosomal, 23S); H1250JIK0A (Clarithromycin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171010
[St] Status:MEDLINE
[do] DOI:10.1177/1010428317734865


  10 / 17205 MEDLINE  
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[PMID]:28957388
[Au] Autor:von Volkmann HL; Brønstad I; Gilja OH; R Tronstad R; Sangnes DA; Nortvedt R; Hausken T; Dimcevski G; Fiskerstrand T; Nylund K
[Ad] Endereço:National Centre for Ultrasound in Gastroenterology, Haukeland University Hospital, Bergen, Norway.
[Ti] Título:Prolonged intestinal transit and diarrhea in patients with an activating GUCY2C mutation.
[So] Source:PLoS One;12(9):e0185496, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Increased intestinal hydration by activation of the epithelial enzyme linked receptor guanylate cyclase C (GC-C) is a pharmacological principle for treating constipation. Activating mutations in the GUCY2C gene encoding GC-C cause Familial GUCY2C diarrhea syndrome (FGDS) which has been diagnosed with severe dysmotility. AIM: To investigate gut motility and hormones before and after a meal in FGDS patients and compare with healthy controls (HC). SUBJECTS AND METHODS: Bristol stool chart and stool frequency was assessed. Before and after a meal occlusive and non-occlusive contractions were obtained using ultrasound. A wireless motility capsule (WMC) recorded gut transit time, pH, contractions and pressure. Plasma levels of selected gut hormones were measured at different time points. RESULTS: The FGDS patients had 4 (range 1-10) loose stools/day and prolonged total gut transit time compared to HC, 55.5 h vs 28.5 h, respectively,with significantly increased colon transit time. In FGDS patients, pH in duodenum, small bowel and colon was increased and the number of contractions and the intraluminal pressure were significantly decreased, measured by WMC. Ultrasound showed in small bowel increased number of non-occlusive contractions in the FGDS patients. Serotonin (5-HT) plasma levels in the HC peaked 30 min after the meal, while the FGDS patients had no response. CONCLUSION: Despite having diarrhea, the FGDS patients have prolonged transit time through the gut compared to HC, particularly in colon. The reduced number of intestinal contractions and lack of 5-HT release after a meal in FGDS patients surprisingly resemble colonic motility disturbances seen in patients with constipation.
[Mh] Termos MeSH primário: Diarreia/genética
Diarreia/fisiopatologia
Trânsito Gastrointestinal/fisiologia
Mutação/genética
Receptores Acoplados a Guanilato Ciclase/genética
Receptores de Peptídeos/genética
[Mh] Termos MeSH secundário: Adulto
Idoso
Líquidos Corporais
Endoscopia por Cápsula
Diarreia/sangue
Diarreia/diagnóstico por imagem
Feminino
Hormônios/sangue
Seres Humanos
Concentração de Íons de Hidrogênio
Intestinos/patologia
Intestinos/fisiopatologia
Masculino
Meia-Idade
Contração Muscular
Variações Dependentes do Observador
Pressão
Receptores de Enterotoxina
Fatores de Tempo
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hormones); 0 (Receptors, Peptide); EC 4.6.1.2 (GUCY2C protein, human); EC 4.6.1.2 (Receptors, Enterotoxin); EC 4.6.1.2 (Receptors, Guanylate Cyclase-Coupled)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170929
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185496



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