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Pesquisa : A12.207.152.693 [Categoria DeCS]
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[PMID]:29431326
[Au] Autor:Khripach LV; Zheleznyak EV; Knyazeva TD; Koganova ZI; Salikhova DI; Grishin DA
[Ti] Título:[Method of colored model radicals for assessment of oxidative equilibrium in biologic samples].
[So] Source:Gig Sanit;95(9):884-90, 2016.
[Is] ISSN:0016-9900
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The most specific method of the recording of the rate offree radical reactions is the method of electron paramagnetic resonance (EPR) spectroscopy, but it is rarely used in applied biology due to expensive equipment and complexity of the execution of measurements. However chemists have found a number of colored organic radicals which lose the coloring under transition into diamagnetic form. In the given paper there are presented results of our studies on the development of methods for the assessment of oxidant equilibrium in biological media with a use of stable radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) and cation-radicals of N,N-diethyl-p-phenylenediamine (DEPPD). We have developed the new modification of DPPH test, replacing methanol-based incubation medium by non-ionic detergent solution, compatible with native blood serum. Modified DPPH test conserved typical biphasic kinetics of the origin variant, had the similar sensitivity to model antioxidants (IC values 49, 38 and 13 mkMfor ascorbate, a-tocopherol and quercetine, correspondingly) and was applied in experiments on laboratory animals treated with nano- and ionic silver, carbon nanotubes, microfine coal and electrolytic dust. We have tried also the assay of serum lipid hydroperoxides based on Fe-initiated DEPPD oxidation (Alberti et al., 2000). The comparison of kinetics of DEPPD oxidation in model (HO/Fe) and biologic (rat serum/Fe) systems, before and after Fe addition, seems to be an evidence that ceruloplasmin (CP) was involved in the resulting process, but failed to determine its polynomial kinetics, at least for the rat serum and DEPPD excess. The use of CP monoclonal antibodies seems to be the best way for the clarification of the mechanism of this reaction.
[Mh] Termos MeSH primário: Compostos de Bifenilo
Oxirredução
Fenilenodiaminas
Picratos
Plasma
[Mh] Termos MeSH secundário: Animais
Fenômenos Bioquímicos
Compostos de Bifenilo/análise
Compostos de Bifenilo/química
Compostos de Bifenilo/metabolismo
Corantes/análise
Corantes/química
Indicadores e Reagentes/análise
Indicadores e Reagentes/química
Modelos Químicos
Fenilenodiaminas/análise
Fenilenodiaminas/química
Fenilenodiaminas/metabolismo
Picratos/análise
Picratos/química
Picratos/metabolismo
Plasma/química
Plasma/metabolismo
Ratos
Kit de Reagentes para Diagnóstico
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biphenyl Compounds); 0 (Coloring Agents); 0 (Indicators and Reagents); 0 (Phenylenediamines); 0 (Picrates); 0 (Reagent Kits, Diagnostic); 93-05-0 (N,N-diethyl 4-phenylenediamine); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180213
[St] Status:MEDLINE


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[PMID]:28826030
[Au] Autor:Ahmed F; Perveen S; Shah K; Shah MR; Ahmed S
[Ad] Endereço:a International Center for Chemical and Biological Sciences, H.E.J. Research Institute of Chemistry, University of Karachi, Karachi 75270, Pakistan; Department of Chemistry, Women University of Azad Jammu & Kashmir Bagh, 12500, Pakistan. Electronic address: farids_ahmed@yahoo.com.
[Ti] Título:Synthesis and characterization of triazole based supramolecule for interaction with cefuroxime in tap water and blood plasma.
[So] Source:Ecotoxicol Environ Saf;147:49-54, 2018 Jan.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In this study a new calix[4]arene triazole 5 was successfully synthesized using click reaction and characterized through UV-visible, FT-IR, H NMR spectroscopes and Mass Spectrometry. The supramolecular interaction of compound 5 towards commonly used drugs has been carried out using UV-Visible spectroscopy. The supramolecule 5 showed characteristic enhancement in the absorbance intensity after mixing with Cefuroxime at pH (2-12). Compound 5 displayed considerably good interactions with cefuroxime in the presence of other drugs. Compound 5 exhibits linear relationship with cefuroxime concentration in the range of (10-80µM) with regression value of 0.9954. The standard deviation for 50µM Cefuroxime was found to be 0.01 and the limit of detection for cefuroxime was calculated to be 2µM. Job's plot experiments showed 1:1 (5: cefuroxime) binding stoichiometry between compound 5 and cefuroxime. Supramolecule 5 displayed fairly good spectrophotometric recognition of Cefuroxime in human blood plasma and tap water thus showing that the ingredients of tap water and plasma sample was inert in the recognition of cefuroxime.
[Mh] Termos MeSH primário: Calixarenos/química
Cefuroxima/sangue
Água Potável/química
Fenóis/química
Triazóis/síntese química
Poluentes Químicos da Água/sangue
[Mh] Termos MeSH secundário: Cefuroxima/análise
Seres Humanos
Técnicas In Vitro
Limite de Detecção
Espectroscopia de Ressonância Magnética
Plasma/química
Espectroscopia de Infravermelho com Transformada de Fourier
Triazóis/química
Poluentes Químicos da Água/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drinking Water); 0 (Phenols); 0 (Triazoles); 0 (Water Pollutants, Chemical); 0 (calix(4)arene); 130036-26-9 (Calixarenes); O1R9FJ93ED (Cefuroxime)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170822
[St] Status:MEDLINE


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[PMID]:29268779
[Au] Autor:Patlán M; Sánchez-Muñoz F; Amezcua-Guerra LM; Granados A; Páez A; Massó F; Mejía AM; Soster A; Bojalil R; Pavón L; Jiménez-Zamudio LA; Márquez-Velasco R
[Ad] Endereço:Doctorado en Ciencias Quimicobiológicas, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, Mexico.
[Ti] Título:Effect of fresh frozen plasma on the in vitro activation of U937 monocytes: a potential role for the age of blood donors and their underlying cytokine profile.
[So] Source:Biol Res;50(1):42, 2017 Dec 21.
[Is] ISSN:0717-6287
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Fresh frozen plasma (FFP) administration may increase the risk of nosocomial infections in parallel with the development of immune modulation. This could be driven by soluble mediators, possibly influencing the in vitro activation of human U937 monocyte cells, in a manner dependent on the age of the donors. METHODS: FFP donors were stratified into groups of 19-30 years, 31-40 years or 41-50 years, and U937 cells were cultured with FFP (alone or plus lipopolysaccharide-LPS) for 24 h. Both in FFP and supernatants, TNF, IL-1ß, IL-6, and IL-10 levels were measured by ELISA. Additionally, CD11B, TLR2, and CASP3 gene expression were measured by qtPCR in U937 cells. Total phagocytic activity was also assayed. RESULTS: Elevated IL-10, but low TNF and IL-1ß levels were measured in FFP from individuals aged 19-40 years, whereas in individuals aged 41-50 years FFP were characterized by equalized TNF and IL-10 levels. Elevated IL-6 levels were found in all FFP samples, especially in those from the oldest individuals. FFP stimulation was associated with striking modifications in cytokine production in an age-dependent way. Exposure to FFP attenuates the response to LPS. TLR2 and CD11B expression were enhanced regardless of the age of plasma donors, although CASP3 expression was increased only when FFP from individuals aged 19-40 years were tested. Phagocytosis decreased after exposure to FFP regardless of donor age. CONCLUSION: Our results suggest that soluble mediators in FFP may modulate the functioning of monocytes. Interestingly, this effect appears to be partially influenced by the age of donors.
[Mh] Termos MeSH primário: Doadores de Sangue
Citocinas/imunologia
Monócitos/imunologia
Plasma/imunologia
Células U937/imunologia
[Mh] Termos MeSH secundário: Adulto
Fatores Etários
Ensaio de Imunoadsorção Enzimática
Feminino
Seres Humanos
Interleucina-10/metabolismo
Interleucina-1beta/metabolismo
Interleucina-6/metabolismo
Masculino
Meia-Idade
Monócitos/fisiologia
Fator de Necrose Tumoral alfa/metabolismo
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 0 (IL10 protein, human); 0 (Interleukin-1beta); 0 (Interleukin-6); 0 (Tumor Necrosis Factor-alpha); 130068-27-8 (Interleukin-10)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171223
[St] Status:MEDLINE
[do] DOI:10.1186/s40659-017-0146-3


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[PMID]:29301740
[Au] Autor:Hunt BJ; Levi M
[Ad] Endereço:King's College, London, UK Beverley.hunt@gstt.nhs.uk.
[Ti] Título:Urgent reversal of vitamin K antagonists.
[So] Source:BMJ;360:j5424, 2018 01 04.
[Is] ISSN:1756-1833
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Fibrilação Atrial/complicações
Encéfalo/diagnóstico por imagem
Hemorragias Intracranianas/induzido quimicamente
Paresia/diagnóstico
Vitamina K/antagonistas & inibidores
Varfarina/efeitos adversos
[Mh] Termos MeSH secundário: Administração Intravenosa
Idoso
Anticoagulantes/uso terapêutico
Fibrilação Atrial/tratamento farmacológico
Encéfalo/patologia
Serviço Hospitalar de Emergência
Fator V/administração & dosagem
Fator V/economia
Fator V/uso terapêutico
Fator Xa/administração & dosagem
Fator Xa/economia
Fator Xa/uso terapêutico
Seres Humanos
Coeficiente Internacional Normatizado/normas
Hemorragias Intracranianas/diagnóstico por imagem
Hemorragias Intracranianas/mortalidade
Masculino
Paresia/etiologia
Plasma
Tomografia Computadorizada por Raios X/métodos
Vitamina K/administração & dosagem
Vitamina K/uso terapêutico
Varfarina/uso terapêutico
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anticoagulants); 0 (prothrombinase complex); 12001-79-5 (Vitamin K); 5Q7ZVV76EI (Warfarin); 9001-24-5 (Factor V); EC 3.4.21.6 (Factor Xa)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180106
[St] Status:MEDLINE
[do] DOI:10.1136/bmj.j5424


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[PMID]:27774620
[Au] Autor:Vendramin C; McGuckin S; Alwan F; Westwood JP; Thomas M; Scully M
[Ad] Endereço:Department of Haematology, University College London Hospital.
[Ti] Título:A single-center prospective study on the safety of plasma exchange procedures using a double-viral-inactivated and prion-reduced solvent/detergent fresh-frozen plasma as the replacement fluid in the treatment of thrombotic microangiopathy.
[So] Source:Transfusion;57(1):131-136, 2017 01.
[Is] ISSN:1537-2995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Patients presenting with acute episodes of thrombotic microangiopathies (TMAs) require urgent access to plasma exchange (PEX). OctaplasLG, a solvent/detergent fresh-frozen plasma product that has undergone viral inactivation and prion reduction step, has been used in our institution since 2013, replacing Octaplas. STUDY DESIGN AND METHODS: We prospectively reviewed 981 PEX procedures where OctaplasLG was the replacement fluid in 90 patients admitted acutely with a TMA presentation within our institution from January 1, 2013, to December 31, 2015. We recorded citrate toxicities, plasma reactions, viral transfer, complications related to central venous catheter, and venous thrombotic events (VTEs). RESULTS: Citrate toxicities were 5.4%, plasma reactions were 2%, and all were classified as Grade 1 or 2. VTE had an incidence of 12.2%, although 50% of the episodes occurred in early remission when patients were not receiving PEX. No line insertions complications were recorded. Line-associated infections were 2.2%. Hepatitis B and C serology and human immunodeficiency virus (HIV) were checked on admission. There were four patients who may have had passive transient transfer of hepatitis B antibodies from pooled plasma. No hepatitis C or HIV viral transfer was documented after treatment and no seroconversion was detected after treatment. CONCLUSION: Our data have demonstrated that the incidence of complications during PEX is low and using OctaplasLG is comparable to the low incidence of reactions. No cases of anaphylaxis, transfusion-related acute lung injury, or fatal plasma reactions were seen. There was no evidence of viral transmission or seroconversion after treatment.
[Mh] Termos MeSH primário: Desinfecção/métodos
Troca Plasmática/métodos
Plasma
Príons
Microangiopatias Trombóticas/terapia
Inativação de Vírus
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Detergentes/química
Feminino
HIV
Infecções por HIV/prevenção & controle
Hepacivirus
Hepatite B/prevenção & controle
Vírus da Hepatite B
Hepatite C/prevenção & controle
Seres Humanos
Masculino
Meia-Idade
Estudos Prospectivos
Solventes/química
[Pt] Tipo de publicação:CLINICAL TRIAL; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Detergents); 0 (Prions); 0 (Solvents)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE
[do] DOI:10.1111/trf.13877


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[PMID]:29235591
[Au] Autor:Chen J; Xu X; Huang Z; Luo Y; Tang L; Jiang JH
[Ad] Endereço:State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China. jianhuijiang@hnu.edu.cn tanglijuang@hnu.edu.cn.
[Ti] Título:BEAMing LAMP: single-molecule capture and on-bead isothermal amplification for digital detection of hepatitis C virus in plasma.
[So] Source:Chem Commun (Camb);54(3):291-294, 2018 Jan 02.
[Is] ISSN:1364-548X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel dNAD platform (BEAMing LAMP) by combining emulsion micro-reactors, single-molecule magnetic capture and on-bead loop-mediated isothermal amplification has been developed for DNA detection, which enables absolute and high-precision quantification of a target with a detection limit of 300 copies.
[Mh] Termos MeSH primário: DNA/sangue
Hepacivirus/isolamento & purificação
Técnicas de Amplificação de Ácido Nucleico/métodos
[Mh] Termos MeSH secundário: Emulsões
Óxido Ferroso-Férrico/química
Fluorescência
Células HeLa
Seres Humanos
Tamanho da Partícula
Plasma
Poliestirenos/química
RNA/sangue
Carga Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Emulsions); 0 (Polystyrenes); 63231-63-0 (RNA); 9007-49-2 (DNA); XM0M87F357 (Ferrosoferric Oxide)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.1039/c7cc08403j


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[PMID]:28449711
[Au] Autor:Blázquez-Prunera A; Díez JM; Gajardo R; Grancha S
[Ad] Endereço:Research and Development, Bioscience Industrial Group, Grifols, Parets del Vallès, Barcelona, Spain.
[Ti] Título:Human mesenchymal stem cells maintain their phenotype, multipotentiality, and genetic stability when cultured using a defined xeno-free human plasma fraction.
[So] Source:Stem Cell Res Ther;8(1):103, 2017 Apr 27.
[Is] ISSN:1757-6512
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Mesenchymal stem cells (MSCs) show promising characteristics for their use in advanced therapy medicinal products. However, there are some unresolved concerns, such as the use of animal components for their expansion. In this study we assessed the suitability of a xeno-free supplement for cell culture (SCC) derived from human plasma, to culture and expand human MSCs (hMSCs) from different origins. Characteristics of viable cultured hMSCs such as genetic stability, phenotype and multipotentiality were qualitatively evaluated. METHODS: hMSCs from adipose tissue (AT), bone marrow (BM) and umbilical cord (UC) and supplier sources (commercial/non-commercial) were used. After hMSCs expansion in a xeno-free medium, classical hMSCs markers were studied by immunocytochemistry, and genetic stability was tested by classic karyotyping. The capacity of hMSCs to differentiate into adipogenic, osteogenic, and chondrogenic cells in differentiation media was assessed using different staining. Different lots of SCC were used to assure consistency between batches. RESULTS: All hMSCs tested maintained their morphology and adherence to plastic during their expansion, and preserved their genetic stability, phenotype and differentiation potential. No differences were observed when using different lots of SCC. Moreover, the proliferation rate, evaluated as population doubling time (PDT) of commercial BM and AT hMSCs, was higher in the xeno-free medium than in the control media provided by the suppliers of the cells (PDT of 4.6 for BM-hMSC and 6.4 for AT-hMSC in xeno-free medium, and 7.0 and 14.7 respectively in the commercial media). UC-hMSCs PDT was similar in all the media tested. When using non-commercial BM-hMSCs, PDT was lower in the xeno-free medium, but reverted to the control level with the addition of growth factors. CONCLUSIONS: SCC-containing medium can be a feasible xeno-free alternative to expand hMSCs for advanced therapies.
[Mh] Termos MeSH primário: Meios de Cultura/farmacologia
Células Mesenquimais Estromais/efeitos dos fármacos
Cultura Primária de Células/métodos
[Mh] Termos MeSH secundário: Diferenciação Celular
Células Cultivadas
Meios de Cultura/química
Instabilidade Genômica
Seres Humanos
Peptídeos e Proteínas de Sinalização Intercelular/farmacologia
Cariótipo
Células Mesenquimais Estromais/citologia
Células Mesenquimais Estromais/metabolismo
Fenótipo
Plasma/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media); 0 (Intercellular Signaling Peptides and Proteins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1186/s13287-017-0552-z


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[PMID]:29346414
[Au] Autor:Harada S; Hirayama A; Chan Q; Kurihara A; Fukai K; Iida M; Kato S; Sugiyama D; Kuwabara K; Takeuchi A; Akiyama M; Okamura T; Ebbels TMD; Elliott P; Tomita M; Sato A; Suzuki C; Sugimoto M; Soga T; Takebayashi T
[Ad] Endereço:Department of Preventive Medicine and Public Health, Keio University School of Medicine, Tokyo, Japan.
[Ti] Título:Reliability of plasma polar metabolite concentrations in a large-scale cohort study using capillary electrophoresis-mass spectrometry.
[So] Source:PLoS One;13(1):e0191230, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Cohort studies with metabolomics data are becoming more widespread, however, large-scale studies involving 10,000s of participants are still limited, especially in Asian populations. Therefore, we started the Tsuruoka Metabolomics Cohort Study enrolling 11,002 community-dwelling adults in Japan, and using capillary electrophoresis-mass spectrometry (CE-MS) and liquid chromatography-mass spectrometry. The CE-MS method is highly amenable to absolute quantification of polar metabolites, however, its reliability for large-scale measurement is unclear. The aim of this study is to examine reproducibility and validity of large-scale CE-MS measurements. In addition, the study presents absolute concentrations of polar metabolites in human plasma, which can be used in future as reference ranges in a Japanese population. METHODS: Metabolomic profiling of 8,413 fasting plasma samples were completed using CE-MS, and 94 polar metabolites were structurally identified and quantified. Quality control (QC) samples were injected every ten samples and assessed throughout the analysis. Inter- and intra-batch coefficients of variation of QC and participant samples, and technical intraclass correlation coefficients were estimated. Passing-Bablok regression of plasma concentrations by CE-MS on serum concentrations by standard clinical chemistry assays was conducted for creatinine and uric acid. RESULTS AND CONCLUSIONS: In QC samples, coefficient of variation was less than 20% for 64 metabolites, and less than 30% for 80 metabolites out of the 94 metabolites. Inter-batch coefficient of variation was less than 20% for 81 metabolites. Estimated technical intraclass correlation coefficient was above 0.75 for 67 metabolites. The slope of Passing-Bablok regression was estimated as 0.97 (95% confidence interval: 0.95, 0.98) for creatinine and 0.95 (0.92, 0.96) for uric acid. Compared to published data from other large cohort measurement platforms, reproducibility of metabolites common to the platforms was similar to or better than in the other studies. These results show that our CE-MS platform is suitable for conducting large-scale epidemiological studies.
[Mh] Termos MeSH primário: Eletroforese Capilar/métodos
Espectrometria de Massas/métodos
Metabolômica/métodos
Plasma/metabolismo
[Mh] Termos MeSH secundário: Adulto
Idoso
Análise de Variância
Grupo com Ancestrais do Continente Asiático
Biomarcadores/sangue
Estudos de Coortes
Feminino
Seres Humanos
Japão
Masculino
Metaboloma
Metabolômica/normas
Meia-Idade
Controle de Qualidade
Valores de Referência
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biomarkers)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180119
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191230


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[PMID]:28457980
[Au] Autor:Innerhofer P; Fries D; Mittermayr M; Innerhofer N; von Langen D; Hell T; Gruber G; Schmid S; Friesenecker B; Lorenz IH; Ströhle M; Rastner V; Trübsbach S; Raab H; Treml B; Wally D; Treichl B; Mayr A; Kranewitter C; Oswald E
[Ad] Endereço:Department of Anaesthesiology and Intensive Care Medicine, Medical University of Innsbruck, Innsbruck, Austria. Electronic address: Petra.Innerhofer@tirol-kliniken.at.
[Ti] Título:Reversal of trauma-induced coagulopathy using first-line coagulation factor concentrates or fresh frozen plasma (RETIC): a single-centre, parallel-group, open-label, randomised trial.
[So] Source:Lancet Haematol;4(6):e258-e271, 2017 Jun.
[Is] ISSN:2352-3026
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Effective treatment of trauma-induced coagulopathy is important; however, the optimal therapy is still not known. We aimed to compare the efficacy of first-line therapy using fresh frozen plasma (FFP) or coagulation factor concentrates (CFC) for the reversal of trauma-induced coagulopathy, the arising transfusion requirements, and consequently the development of multiple organ failure. METHODS: This single-centre, parallel-group, open-label, randomised trial was done at the Level 1 Trauma Center in Innsbruck Medical University Hospital (Innsbruck, Austria). Patients with trauma aged 18-80 years, with an Injury Severity Score (ISS) greater than 15, bleeding signs, and plasmatic coagulopathy identified by abnormal fibrin polymerisation or prolonged coagulation time using rotational thromboelastometry (ROTEM) were eligible. Patients with injuries that were judged incompatible with survival, cardiopulmonary resuscitation on the scene, isolated brain injury, burn injury, avalanche injury, or prehospital coagulation therapy other than tranexamic acid were excluded. We used a computer-generated randomisation list, stratification for brain injury and ISS, and closed opaque envelopes to randomly allocate patients to treatment with FFP (15 mL/kg of bodyweight) or CFC (primarily fibrinogen concentrate [50 mg/kg of bodyweight]). Bleeding management began immediately after randomisation and continued until 24 h after admission to the intensive care unit. The primary clinical endpoint was multiple organ failure in the modified intention-to-treat population (excluding patients who discontinued treatment). Reversal of coagulopathy and need for massive transfusions were important secondary efficacy endpoints that were the reason for deciding the continuation or termination of the trial. This trial is registered with ClinicalTrials.gov, number NCT01545635. FINDINGS: Between March 3, 2012, and Feb 20, 2016, 100 out of 292 screened patients were included and randomly allocated to FFP (n=48) and CFC (n=52). Six patients (four in the FFP group and two in the CFC group) discontinued treatment because of overlooked exclusion criteria or a major protocol deviation with loss of follow-up. 44 patients in the FFP group and 50 patients in the CFC group were included in the final interim analysis. The study was terminated early for futility and safety reasons because of the high proportion of patients in the FFP group who required rescue therapy compared with those in the CFC group (23 [52%] in the FFP group vs two [4%] in the CFC group; odds ratio [OR] 25·34 [95% CI 5·47-240·03], p<0·0001) and increased needed for massive transfusion (13 [30%] in the FFP group vs six [12%] in the CFC group; OR 3·04 [0·95-10·87], p=0·042) in the FFP group. Multiple organ failure occurred in 29 (66%) patients in the FFP group and in 25 (50%) patients in the CFC group (OR 1·92 [95% CI 0·78-4·86], p=0·15). INTERPRETATION: Our results underline the importance of early and effective fibrinogen supplementation for severe clotting failure in multiple trauma. The available sample size in our study appears sufficient to make some conclusions that first-line CFC is superior to FFP. FUNDING: None.
[Mh] Termos MeSH primário: Fatores de Coagulação Sanguínea/uso terapêutico
Hemorragia/tratamento farmacológico
Hemorragia/etiologia
Plasma
Ferimentos e Lesões/complicações
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Feminino
Seres Humanos
Masculino
Meia-Idade
Resultado do Tratamento
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Blood Coagulation Factors)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[Cl] Clinical Trial:ClinicalTrial
[St] Status:MEDLINE


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[PMID]:28987408
[Au] Autor:Liu QS; Hao F; Sun Z; Long Y; Zhou Q; Jiang G
[Ad] Endereço:State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, PR China; College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, 100049, PR China.
[Ti] Título:Perfluorohexadecanoic acid increases paracellular permeability in endothelial cells through the activation of plasma kallikrein-kinin system.
[So] Source:Chemosphere;190:191-200, 2018 Jan.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Per- and polyfluoroalkyl substances (PFASs) are ubiquitous and high persistent in human blood, thus potentially inducing a myriad of deleterious consequences. Plasma kallikrein-kinin system (KKS), which physiologically regulates vascular permeability, is vulnerable to exogenous stimulators, like PFASs with long-chain alkyl backbone substituted by electronegative fluorine. The study on the interactions of PFASs with the KKS and the subsequent effects on vascular permeability would be helpful to illustrate how the chemicals penetrate the biological vascular barriers to reach different tissues. In present study, three representative PFASs, including perfluorooctane sulfonic acid (PFOS), perfluorooctanoic acid (PFOA) and perfluorohexadecanoic acid (PFHxDA), were investigated for their effects on the activation of the KKS, paracellular permeability in human retina endothelial cells (HRECs) and integrity of the adherens junctions. In contrast to either PFOS or PFOA, PFHxDA efficiently triggered KKS activation in a concentration-dependent manner based on protease activity assays. The plasma activated by PFHxDA significantly increased paracellular permeability of HRECs through the degradation of adherens junctions. As evidenced by the antagonistic effect of aprotinin, PFHxDA-involved effects on vascular permeability were mediated by KKS activation. The results herein firstly revealed the mechanistic pathway for PFHxDA induced effects on vascular endothelial cells. Regarding the possible structure-related activities of the chemicals, this finding would be of great help in the risk assessment of PFASs.
[Mh] Termos MeSH primário: Permeabilidade Capilar/efeitos dos fármacos
Células Endoteliais/metabolismo
Fluorcarbonetos/farmacologia
Sistema Calicreína-Cinina/efeitos dos fármacos
Ácido Palmítico/farmacologia
[Mh] Termos MeSH secundário: Junções Aderentes/metabolismo
Ácidos Alcanossulfônicos/farmacologia
Caprilatos/farmacologia
Células Cultivadas
Células Endoteliais/fisiologia
Seres Humanos
Sistema Calicreína-Cinina/fisiologia
Plasma/efeitos dos fármacos
Retina/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkanesulfonic Acids); 0 (Caprylates); 0 (Fluorocarbons); 2V16EO95H1 (Palmitic Acid); 947VD76D3L (perfluorooctanoic acid); 9H2MAI21CL (perfluorooctane sulfonic acid)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171009
[St] Status:MEDLINE



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