Base de dados : MEDLINE
Pesquisa : A12.383.250 [Categoria DeCS]
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  1 / 202 MEDLINE  
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[PMID]:26846037
[Au] Autor:Nawareg MM; Zidan AZ; Zhou J; Chiba A; Tagami J; Pashley DH
[Ti] Título:Adhesive sealing of dentin surfaces in vitro: A review.
[So] Source:Am J Dent;28(6):321-32, 2015 Dec.
[Is] ISSN:0894-8275
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PURPOSE: This review describes the evolution of the use of dental adhesives to form a tight seal of freshly prepared dentin to protect the pulp from bacterial products, during the time between crown preparation and final cementation of full crowns. The evolution of these "immediate dentin sealants" follows the evolution of dental adhesives, in general. That is, they began with multiple-step, etch-and-rinse adhesives, and then switched to the use of simplified adhesives. METHODS: Literature was reviewed for evidence that bacteria or bacterial products diffusing across dentin can irritate pulpal tissues before and after smear layer removal. Smear layers can be solubilized by plaque organisms within 7-10 days if they are directly exposed to oral fluids. It is likely that smear layers covered by temporary restorations may last more than 1 month. As long as smear layers remain in place, they can partially seal dentin. Thus, many in vitro studies evaluating the sealing ability of adhesive resins use smear layer-covered dentin as a reference condition. Surprisingly, many adhesives do not seal dentin as well as do smear layers. RESULTS: Both in vitro and in vivo studies show that resin- covered dentin allows dentin fluid to cross polymerized resins. The use of simplified single bottle adhesives to seal dentin was a step backwards. Currently, most authorities use either 3-step adhesives such as Scotchbond Multi-Purpose or OptiBond FL or two-step self-etching primer adhesives, such as Clearfil SE, Unifil Bond or AdheSE.
[Mh] Termos MeSH primário: Colagem Dentária
Adesivos Dentinários/química
Dentina/ultraestrutura
Cimentos de Resina/química
[Mh] Termos MeSH secundário: Permeabilidade da Dentina/fisiologia
Adesivos Dentinários/classificação
Líquido Dentinal/metabolismo
Seres Humanos
Teste de Materiais
Cimentos de Resina/classificação
Camada de Esfregaço/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (Dentin-Bonding Agents); 0 (Resin Cements)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:160206
[St] Status:MEDLINE


  2 / 202 MEDLINE  
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[PMID]:26714349
[Au] Autor:Kim MH; Kim RJ; Lee WC; Lee IB
[Ti] Título:Evaluation of dentin tubule occlusion after laser irradiation and desensitizing agent application.
[So] Source:Am J Dent;28(5):303-8, 2015 Oct.
[Is] ISSN:0894-8275
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PURPOSE: To evaluate the effects of lasers (Nd:YAG and Er:YAG) and of topical desensitizing agents on dentin tubule occlusion by measuring real-time dentin fluid flow (DFF). METHODS: 32 molars were prepared with V-shape cavity at the cervical area, acid-etched, water rinsed, blotted dry, and treated with (1) Nd:YAG laser; (2) Er:YAG laser; (3) SuperSeal, a desensitizing agent; (4) ClinproXT, a resin-modified glass-ionomer (RMGI) varnish (n = 8 each). A real-time fluid flow measuring instrument (nano-Flow) was used to measure the DFF throughout the procedures. The DFF rates before and after the treatment were compared. Moreover, the surface topography of dentin tubules after each desensitizing method was examined using SEM. RESULTS: DFF varied among the groups. The DFF rate was significantly reduced after laser irradiation/application of the desensitizing agents (P < 0.05). ClinproXT showed the greatest reduction of DFF rate (71.9%), followed by the SuperSeal (34.8%) and laser groups (P< 0.05). However, there was no significant difference between the Nd:YAG (24.1%) and Er:YAG (20.6%) groups (P > 0.05). In SEM images, narrowed dentin tubules were observed in both lased groups and SuperSeal group. In the ClinproXT group, the occluded dentin tubules by the RMGI covering were observed.
[Mh] Termos MeSH primário: Dessensibilizantes Dentinários/uso terapêutico
Dentina/ultraestrutura
Líquido Dentinal/fisiologia
Lasers de Estado Sólido/uso terapêutico
[Mh] Termos MeSH secundário: Ataque Ácido Dentário/métodos
Preparo da Cavidade Dentária/classificação
Dentina/efeitos dos fármacos
Dentina/efeitos da radiação
Sensibilidade da Dentina/fisiopatologia
Líquido Dentinal/efeitos dos fármacos
Líquido Dentinal/efeitos da radiação
Cimentos de Ionômeros de Vidro/química
Seres Humanos
Hidrodinâmica
Microscopia Eletrônica de Varredura
Ácido Oxálico/uso terapêutico
Distribuição Aleatória
Cimentos de Resina/química
Colo do Dente/ultraestrutura
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dentin Desensitizing Agents); 0 (Glass Ionomer Cements); 0 (Resin Cements); 0 (SuperSeal); 9E7R5L6H31 (Oxalic Acid)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:151230
[Lr] Data última revisão:
151230
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:151231
[St] Status:MEDLINE


  3 / 202 MEDLINE  
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[PMID]:26287044
[Ti] Título:Arginine Bicarbonate/Calcium Carbonate Soft Chews Seal Open Dentinal Tubules.
[So] Source:Dent Today;34(7):38, 40, 2015 Jul.
[Is] ISSN:8750-2186
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Arginina/uso terapêutico
Carbonato de Cálcio/uso terapêutico
Dessensibilizantes Dentinários/uso terapêutico
Sensibilidade da Dentina/tratamento farmacológico
Dentina/efeitos dos fármacos
Fluoretos/uso terapêutico
Fosfatos/uso terapêutico
[Mh] Termos MeSH secundário: Líquido Dentinal/efeitos dos fármacos
Seres Humanos
[Pt] Tipo de publicação:NEWS
[Nm] Nome de substância:
0 (Dentin Desensitizing Agents); 0 (Phosphates); 0 (pro-argin); 94ZLA3W45F (Arginine); H0G9379FGK (Calcium Carbonate); Q80VPU408O (Fluorides)
[Em] Mês de entrada:1509
[Cu] Atualização por classe:150819
[Lr] Data última revisão:
150819
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:150820
[St] Status:MEDLINE


  4 / 202 MEDLINE  
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[PMID]:26039461
[Au] Autor:Oh DX; Prajatelistia E; Ju SW; Jeong Kim H; Baek SJ; Joon Cha H; Ho Jun S; Ahn JS; Soo Hwang D
[Ad] Endereço:POSTECH Ocean Science and Technology, Pohang University of Science and Technology (POSTECH), Pohang 790-784, Korea.
[Ti] Título:A rapid, efficient, and facile solution for dental hypersensitivity: The tannin-iron complex.
[So] Source:Sci Rep;5:10884, 2015 Jun 03.
[Is] ISSN:2045-2322
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Dental hypersensitivity due to exposure of dentinal tubules under the enamel layer to saliva is a very popular and highly elusive technology priority in dentistry. Blocking water flow within exposed dentinal tubules is a key principle for curing dental hypersensitivity. Some salts used in "at home" solutions remineralize the tubules inside by concentrating saliva ingredients. An "in-office" option of applying dense resin sealants on the tubule entrance has only localized effects on well-defined sore spots. We report a self-assembled film that was formed by facile, rapid (4 min), and efficient (approximately 0.5 g/L concentration) dip-coating of teeth in an aqueous solution containing a tannic acid-iron(III) complex. It quickly and effectively occluded the dentinal tubules of human teeth. It withstood intense tooth brushing and induced hydroxyapatite remineralisation within the dentinal tubules. This strategy holds great promise for future applications as an effective and user-friendly desensitizer for managing dental hypersensitivity.
[Mh] Termos MeSH primário: Sensibilidade da Dentina/tratamento farmacológico
Ferro
Soluções Farmacêuticas/administração & dosagem
Taninos
[Mh] Termos MeSH secundário: Técnicas de Cultura de Células
Líquido Dentinal/metabolismo
Seres Humanos
Ferro/química
Ligamento Periodontal/citologia
Soluções Farmacêuticas/química
Taninos/química
Dente/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Pharmaceutical Solutions); 0 (Tannins); E1UOL152H7 (Iron)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150604
[St] Status:MEDLINE
[do] DOI:10.1038/srep10884


  5 / 202 MEDLINE  
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[PMID]:25455134
[Au] Autor:Boreak N; Ishihata H; Shimauchi H
[Ad] Endereço:Division of Periodontology and Endodontology, Tohoku University Graduate School of Dentistry, 4-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Miyagi, Japan; Endodontics Department of Dental College, Jazan University, Saudi Arabia.
[Ti] Título:A photochemical method for in vitro evaluation of fluid flow in human dentine.
[So] Source:Arch Oral Biol;60(1):193-8, 2015 Jan.
[Is] ISSN:1879-1506
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To evaluate the flow dynamics of dentine fluid using a chemiluminescence method in vitro. MATERIALS AND METHODS: Horizontally sliced coronal dentine specimens with thicknesses of 1.4, 1.6, 1.8, and 2.0mm (n=10 each) were prepared from extracted human third molars. After cleaning with EDTA, a mounted specimen was clamped between 2 acrylic chambers attached to both the occlusal and pulpal sides. The occlusal chamber, which was closed with a glass coverslip, was filled with a chemiluminescent solution (0.02% luminol and 1% sodium hydroxide in water). A trigger solution of 1% hydrogen peroxide and 1% potassium ferricyanide was injected into the pulpal chamber at a constant pressure of 2.5 kPa, and allowed to immediately flow into the patent dentinal tubules. Four consecutive measurements (T1-T4) were performed on each sample by recording the emission of chemiluminescence with a photodetector. The relationship between the crossing time of the liquid through the slice and dentine thickness was examined. RESULTS: An apparent time delay was detected between the starting points of the trigger solution run and photochemical emission at T1. Dt (Dt, s) values of each thickness group were 13.6 ± 4.25 for 1.4mm, 18.1 ± 2.38 for 1.6mm, 28.0 ± 2.46 for 1.8mm, and 39.2 ± 8.61 for 2.0mm, respectively. Dt significantly decreased as dentine became thinner towards the pulp chamber (P<0.001). CONCLUSIONS: The velocity of fluid flow increased both with increasing dentine depth or reduction of remaining dentine thickness.
[Mh] Termos MeSH primário: Permeabilidade da Dentina/fisiologia
Dentina/fisiologia
Reologia/instrumentação
[Mh] Termos MeSH secundário: Líquido Dentinal/fisiologia
Seres Humanos
Técnicas In Vitro
Medições Luminescentes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1508
[Cu] Atualização por classe:141203
[Lr] Data última revisão:
141203
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:141203
[St] Status:MEDLINE


  6 / 202 MEDLINE  
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Loguercio, Alessandro Dourado
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[PMID]:25264549
[Au] Autor:Mena-Serrano A; Costa TR; Patzlaff RT; Loguercio AD; Reis A
[Ti] Título:Effect of sonic application mode on the resin-dentin bond strength and dentin permeability of self-etching systems.
[So] Source:J Adhes Dent;16(5):435-40, 2014 Oct.
[Is] ISSN:1461-5185
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: To compare manual and sonic adhesive application modes in terms of the permeability and microtensile bond strength of a self-etching adhesive applied in the one-step or two-step protocol. MATERIALS AND METHODS: Self-etching All Bond SE (Bisco) was applied as a one- or a two-step adhesive under manual or sonic vibration modes on flat occlusal dentin surfaces of 64 human molars. Half of the teeth were used to measure the hydraulic conductance of dentin at 200 cm H2O hydrostatic pressure for 5 min immediately after the adhesive application. In the other half, composite buildups (Opallis) were constructed incrementally to create resin-dentin sticks with a cross-sectional area of 0.8 mm² to be tested in tension (0.5 mm/min) immediately after restoration placement. Data were analyzed using a two-way ANOVA and Tukey's test (α = 0.05). RESULTS: The fluid conductance of dentin was significantly reduced by the sonic vibration mode for both adhesives, but no effect on the bond strength values was observed for either adhesive. CONCLUSION: The sonic application mode at an oscillating frequency of 170 Hz can reduce the fluid conductance of the one- and two-step All Bond SE adhesive when applied on dentin.
[Mh] Termos MeSH primário: Colagem Dentária
Permeabilidade da Dentina/fisiologia
Adesivos Dentinários/química
Dentina/ultraestrutura
Sonicação/métodos
[Mh] Termos MeSH secundário: Ataque Ácido Dentário/métodos
Resinas Compostas/química
Materiais Dentários/química
Análise do Estresse Dentário/instrumentação
Líquido Dentinal/fisiologia
Seres Humanos
Pressão Hidrostática
Metacrilatos/química
Ácidos Fosfóricos/química
Sonicação/instrumentação
Estresse Mecânico
Propriedades de Superfície
Resistência à Tração
Fatores de Tempo
Vibração
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (All-Bond system); 0 (Composite Resins); 0 (Dental Materials); 0 (Dentin-Bonding Agents); 0 (Methacrylates); 0 (Phosphoric Acids); E4GA8884NN (phosphoric acid)
[Em] Mês de entrada:1501
[Cu] Atualização por classe:141124
[Lr] Data última revisão:
141124
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:140930
[St] Status:MEDLINE
[do] DOI:10.3290/j.jad.a32810


  7 / 202 MEDLINE  
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[PMID]:25208362
[Au] Autor:Cannabrava VP; Fernandes SL; Calabria MP; Magalhlães AC; Ishikiriama SK; Atta MT; Wang L
[Ti] Título:Bleaching technique effect on dentin permeability.
[So] Source:Am J Dent;27(3):145-8, 2014 Jun.
[Is] ISSN:0894-8275
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PURPOSE: To evaluate the effect of bleaching techniques on dentin permeability. The hypothesis was that the bleaching agent associated or not to etching and/or energy source would be able to increase dentin permeability (conductance). METHODS: Fifty 1 mm-thick disks of mid-coronal dentin were obtained from human third molars, which were mounted in a filtration chamber, allowing exposure of a standardized area of 0.282 mm2. All specimens were treated with 35% liquid phosphoric acid for 15 seconds to maximize dentin permeability. Then they were randomly allocated to five different conditions: C: placebo gel (negative control); HP: Whiteness HP Maxx (WM)-35% hydrogen peroxide; PA-HP: WM preceded by phosphoric acid etching for 15 seconds. HP-E: WM associated to a light source and PA-HP-E: WM preceded by phosphoric acid etching for 15 seconds and associated to a light source. The bleaching agent was applied for 10 minutes with intermediate agitation at 5 minutes. In the groups associated with a light source, it was activated for 30 seconds every 5 minutes. Afterwards, the permeability (Lp) was measured using the Flodec device. The data were collected and analyzed with one-way ANOVA and Tukey's test (P< 0.05). RESULTS: Mean and standard deviation of Lp were: C = 1.92 (1.04); HP = 1.14 (0.29); PA-HP = 14.40 (8.62); HP-E = 4.18 (5.14); PA-HP-E = 27.32 (13.24). Data revealed that phosphoric acid etching could increase the dentin permeability, while the bleaching agent or light curing alone did not.
[Mh] Termos MeSH primário: Permeabilidade da Dentina/efeitos dos fármacos
Clareadores Dentários/farmacologia
Clareamento Dental/métodos
[Mh] Termos MeSH secundário: Ataque Ácido Dentário/métodos
Compostos Inorgânicos de Carbono/química
Dentina/efeitos dos fármacos
Líquido Dentinal/efeitos dos fármacos
Seres Humanos
Peróxido de Hidrogênio/farmacologia
Luz
Ácidos Fosfóricos/farmacologia
Placebos
Compostos de Silício/química
Camada de Esfregaço
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Carbon Compounds, Inorganic); 0 (Phosphoric Acids); 0 (Placebos); 0 (Silicon Compounds); 0 (Tooth Bleaching Agents); BBX060AN9V (Hydrogen Peroxide); E4GA8884NN (phosphoric acid); WXQ6E537EW (silicon carbide)
[Em] Mês de entrada:1409
[Cu] Atualização por classe:140911
[Lr] Data última revisão:
140911
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:140912
[St] Status:MEDLINE


  8 / 202 MEDLINE  
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[PMID]:25208361
[Au] Autor:Dall'Orologio GD; Ishihata H; Finger WJ; Sasaki K
[Ti] Título:In vitro and in vivo evaluation of the effectiveness of three dentin desensitizing treatment regimens.
[So] Source:Am J Dent;27(3):139-44, 2014 Jun.
[Is] ISSN:0894-8275
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PURPOSE: To evaluate the in vitro and in vivo effects of three treatment regimens on dentin permeability and reduction of dentin hypersensitivity (DH). METHODS: The desensitization treatments were: Gluma Desensitizer PowerGel (GLU), MS Coat One (MSC), and dentin burnishing with fiber-resin burs (STB). A split-chamber device was used to determine the permeability of dentin slices cut from human molars in vitro. Fluid flow through dentin was recorded with a photochemical method after EDTA cleaning, albumin soaking and desensitization treatment (n = 10). 61 study participants with three severely hypersensitive teeth each were enrolled. Sensitivity was determined with an air stimulus before, immediately after treatment, and after 1, 3 and 6 months, using a verbal rating scale. RESULTS: From the 61 study participants enrolled, 52 completed the trial. Permeability at baseline and after albumin soaking was not significantly different. All treatments produced reduced fluid flow through dentin (P > 0.05). All treatments reduced DH significantly (no or moderate sensitivity). Statistical results revealed significant differences among the treatments (P = 0.03). Mann-Whitney comparisons showed GLU, STB < MSC.
[Mh] Termos MeSH primário: Dessensibilizantes Dentinários/uso terapêutico
Permeabilidade da Dentina/efeitos dos fármacos
Sensibilidade da Dentina/tratamento farmacológico
[Mh] Termos MeSH secundário: Adulto
Albuminas/farmacologia
Quelantes/farmacologia
Profilaxia Dentária/instrumentação
Dentina/efeitos dos fármacos
Líquido Dentinal/efeitos dos fármacos
Ácido Edético/farmacologia
Resinas Epóxi/química
Feminino
Seguimentos
Vidro/química
Glutaral/uso terapêutico
Seres Humanos
Masculino
Metacrilatos/uso terapêutico
Meia-Idade
Ácido Oxálico/uso terapêutico
Percepção da Dor/efeitos dos fármacos
Polimetil Metacrilato/uso terapêutico
Poliestirenos/uso terapêutico
Zircônio/química
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Albumins); 0 (Chelating Agents); 0 (Dentin Desensitizing Agents); 0 (Epoxy Resins); 0 (Methacrylates); 0 (Polystyrenes); 0 (fiberglass); 0 (gluma desensitizer); 70KO0R01RY (polystyrene sulfonic acid); 9011-14-7 (Polymethyl Methacrylate); 9E7R5L6H31 (Oxalic Acid); 9G34HU7RV0 (Edetic Acid); C6V6S92N3C (Zirconium); T3C89M417N (Glutaral)
[Em] Mês de entrada:1409
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:140912
[St] Status:MEDLINE


  9 / 202 MEDLINE  
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[PMID]:24993261
[Au] Autor:West N; Seong J; Davies M
[Ad] Endereço:Periodontology, Clinical Trials Unit, Bristol Dental School, Bristol, UK.
[Ti] Título:Dentine hypersensitivity.
[So] Source:Monogr Oral Sci;25:108-22, 2014.
[Is] ISSN:0077-0892
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Dentine hypersensitivity is a common oral pain condition affecting many individuals. The aetiology is multifactorial; however, over recent years the importance of erosion has become more evident. For dentine hypersensitivity to occur, the lesion must first be localised on the tooth surface and then initiated to exposed dentine tubules which are patent to the pulp. The short, sharp pain symptom is thought to be derived from the hydrodynamic pain theory and, although transient, is arresting, affecting quality of life. This episodic pain condition is likely to become a more frequent dental complaint in the future due to the increase in longevity of the dentition and the rise in tooth wear, particularly amongst young adults. Many efficacious treatment regimens are now available, in particular a number of over-the-counter home use products. The basic principles of treatment are altering fluid flow in the dentinal tubules with tubule occlusion or modifying or chemically blocking the pulpal nerve.
[Mh] Termos MeSH primário: Sensibilidade da Dentina/etiologia
Erosão Dentária/complicações
[Mh] Termos MeSH secundário: Polpa Dentária/inervação
Dentina/ultraestrutura
Dessensibilizantes Dentinários/uso terapêutico
Sensibilidade da Dentina/patologia
Sensibilidade da Dentina/fisiopatologia
Líquido Dentinal/fisiologia
Seres Humanos
Reologia
Erosão Dentária/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Dentin Desensitizing Agents)
[Em] Mês de entrada:1409
[Cu] Atualização por classe:140704
[Lr] Data última revisão:
140704
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:140705
[St] Status:MEDLINE
[do] DOI:10.1159/000360749


  10 / 202 MEDLINE  
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[PMID]:24681278
[Au] Autor:Zehnder M; Rechenberg DK; Bostanci N; Sisman F; Attin T
[Ad] Endereço:Clinic for Preventive Dentistry, Periodontology, and Cariology, University of Zürich, Center of Dental Medicine, Plattenstrasse 11, CH-8032 Zürich, Switzerland. Electronic address: matthias.zehnder@zzm.uzh.ch.
[Ti] Título:Comparison of vehicles to collect dentinal fluid for molecular analysis.
[So] Source:J Dent;42(8):1027-32, 2014 Aug.
[Is] ISSN:1879-176X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: To test the hypothesis that a material with higher water absorption than polyvinylidene fluoride (PVDF) could increase the yield of target molecules from exposed dentine. METHODS: In a series of standard tests, different cellulose membranes were compared to a PVDF counterpart for their ability to absorb water and release protein. In a subsequent randomized clinical trial, the cellulose material with the most favourable values was compared to PVDF regarding the levels of MMP-2 that could be collected from exposed dentine of healthy human teeth during filling replacement. MMP-2 levels were determined by enzyme-linked immunosorbent assay (ELISA). Data from the laboratory experiments were compared between materials using the appropriate parametric tests. The frequency of cases yielding quantifiable levels of MMP-2 was compared between materials by Fisher's exact test. The level of significance was set at 5%. RESULTS: The cellulose membrane with the largest pore size (12-15µm) absorbed significantly (P<0.05) more water than PVDF. It showed a protein release that was similar to that of PVDF, while the cellulose membranes with smaller pore size retained significantly more protein (P<0.05). Using the large-pore cellulose membrane, MMP-2 could be collected at a quantifiable level from the dentine of healthy teeth in 9 of 13 cases, compared to 1 of 13 with the PVDF membrane (P<0.05). CONCLUSIONS: Under the current conditions, a large-pore cellulose membrane yielded more of a molecule of diagnostic value compared to a standard PVDF membrane. CLINICAL SIGNIFICANCE: Molecular diagnostics of dentinal fluid are hampered by low yields. In the current study, it was shown that cellulose membranes are more useful to collect MMP-2 from dentinal fluid than PVDF membranes.
[Mh] Termos MeSH primário: Líquido Dentinal/química
Membranas Artificiais
Manejo de Espécimes/instrumentação
[Mh] Termos MeSH secundário: Absorção Fisico-Química
Adulto
Celulose/química
Restauração Dentária Permanente
Dentina/enzimologia
Líquido Dentinal/enzimologia
Feminino
Seres Humanos
Masculino
Metaloproteinase 2 da Matriz/análise
Filtros Microporos
Meia-Idade
Polivinil/química
Porosidade
Proteínas/análise
Distribuição Aleatória
Retratamento
Água/química
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Membranes, Artificial); 0 (Polyvinyls); 0 (Proteins); 059QF0KO0R (Water); 24937-79-9 (polyvinylidene fluoride); 9004-34-6 (Cellulose); EC 3.4.24.24 (MMP2 protein, human); EC 3.4.24.24 (Matrix Metalloproteinase 2)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:140722
[Lr] Data última revisão:
140722
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:140401
[St] Status:MEDLINE



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