Base de dados : MEDLINE
Pesquisa : A12.580 [Categoria DeCS]
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  1 / 2016 MEDLINE  
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[PMID]:28877064
[Au] Autor:Ueno H; Kanemitsu Y; Sekine S; Ishiguro M; Ito E; Hashiguchi Y; Kondo F; Shimazaki H; Mochizuki S; Kajiwara Y; Shinto E; Yamamoto J
[Ad] Endereço:Departments of *Surgery **Laboratory Medicine, National Defense Medical College, Saitama Departments of †Colorectal Surgery Division, National Cancer Center Hospital ‡Molecular Pathology Division, National Cancer Center Research Institute §Department of Colorectal Surgery, Tokyo Medical and Dental University Medical Hospital ∥Department of Pathology, Tokyo Medical and Dental University ¶Department of Surgery, Teikyo University School of Medicine #Department of Pathology, Teikyo University Hospital, Tokyo, Japan.
[Ti] Título:Desmoplastic Pattern at the Tumor Front Defines Poor-prognosis Subtypes of Colorectal Cancer.
[So] Source:Am J Surg Pathol;41(11):1506-1512, 2017 Nov.
[Is] ISSN:1532-0979
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Although recent findings of cancer biology research indicate that prognostic power arises from genes expressed by stromal cells rather than epithelial cells, desmoplastic reaction (DR) has not been completely examined as a prognostic marker for colorectal cancer. A pathologic review of 821 stage II and III patients who underwent R0 resection for colorectal cancer at 4 independent institutions was conducted. DR was classified as mature, intermediate, or immature based on the existence of hyalinized keloid-like collagen and myxoid stroma at the extramural desmoplastic front. Totally, 325, 282, and 214 patients were classified as having mature, intermediate, and immature DR, respectively. DR significantly influenced the recurrence rate in the liver, lung, and peritoneum (P≤0.0001 to 0.01). Five-year relapse-free survival (RFS) rate was the highest in the mature group (85.7%), followed by the intermediate (77.3%) and immature (50.4%) groups. A significant adverse impact of immature stroma on RFS was observed in subset analyses of the 4 institutions. Multivariate analysis revealed that DR, along with T and N stages, is an independent prognostic factor. On the basis of Harrell's concordance index, the prognostic power of DR categorization (0.67) in stratifying RFS was greater than any other conventional prognostic factors, including TNM (0.64), N (0.62) and T stages (0.59), venous invasion (0.59), and tumor grade (0.54). Characterizing DR based on the histologic products of activated fibroblasts is valuable for evaluating prognostic outcomes. To our knowledge, this is the first study reporting a greater prognostic power of histology of the fibrotic stroma than that of tumor factors.
[Mh] Termos MeSH primário: Fibroblastos Associados a Câncer/patologia
Neoplasias Colorretais/patologia
Células Estromais/patologia
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Biomarcadores Tumorais/análise
Biópsia
Fibroblastos Associados a Câncer/química
Distribuição de Qui-Quadrado
Colectomia
Colágeno/análise
Neoplasias Colorretais/química
Neoplasias Colorretais/cirurgia
Intervalo Livre de Doença
Matriz Extracelular/química
Matriz Extracelular/patologia
Feminino
Seres Humanos
Hialina/química
Estimativa de Kaplan-Meier
Neoplasias Hepáticas/secundário
Neoplasias Pulmonares/secundário
Masculino
Meia-Idade
Análise Multivariada
Invasividade Neoplásica
Estadiamento de Neoplasias
Neoplasias Peritoneais/secundário
Modelos de Riscos Proporcionais
Fatores de Risco
Células Estromais/química
Fatores de Tempo
Tóquio
Resultado do Tratamento
Microambiente Tumoral
Adulto Jovem
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; MULTICENTER STUDY
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 9007-34-5 (Collagen)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE
[do] DOI:10.1097/PAS.0000000000000946


  2 / 2016 MEDLINE  
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[PMID]:28424236
[Au] Autor:Saglietti C; Piana S; La Rosa S; Bongiovanni M
[Ad] Endereço:Service of Clinical Pathology, Lausanne University Hospital, Institute of Pathology, Lausanne, Switzerland.
[Ti] Título:Hyalinizing trabecular tumour of the thyroid: fine-needle aspiration cytological diagnosis and correlation with histology.
[So] Source:J Clin Pathol;70(8):641-647, 2017 Aug.
[Is] ISSN:1472-4146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Hyalinizing trabecular tumour (HTT) is a rare thyroid neoplasm of follicular cell origin characterised by a trabecular growth pattern and prominent intratrabecular and intertrabecular hyalinisation. These peculiar histological features allow the prompt recognition of this neoplasm in surgical specimens. However, cytological diagnosis of HTT remains elusive and misleading because of overlapping characteristics with other thyroid tumours, particularly papillary thyroid carcinoma (PTC), medullary thyroid carcinoma (MTC) and the newly described non-invasive follicular thyroid neoplasm with papillary-like nuclear features (NIFTP). Nevertheless, the proper recognition of this neoplasm on preoperative cytological preparations is important to avoid unnecessary overtreatment of this indolent lesion. A thorough review of the literature has revealed that the correct diagnosis of HTT in cytological smears is achieved in only 8% of cases. In a further 6% of cases, diagnostic doubt has been indicated. Sixty percent of published cases of HTT have been misdiagnosed as suggestive, suspicious or positive for PTC. These findings underline the difficulties of a cytological-based diagnosis of such entity. In this article we review the cytomorphological features of HTT and their correlation to histological features to provide the reader with the tools to improve diagnostic performance in the identification of HTT on preoperative cytology.
[Mh] Termos MeSH primário: Adenoma/patologia
Neoplasias da Glândula Tireoide/patologia
[Mh] Termos MeSH secundário: Adenoma/genética
Adenoma/terapia
Biópsia por Agulha Fina/métodos
Diagnóstico Diferencial
Rearranjo Gênico
Seres Humanos
Hialina/metabolismo
Imuno-Histoquímica
Receptores Patched/genética
Proteínas Proto-Oncogênicas c-ret/genética
Glândula Tireoide/patologia
Neoplasias da Glândula Tireoide/genética
Neoplasias da Glândula Tireoide/terapia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Patched Receptors); EC 2.7.10.1 (Proto-Oncogene Proteins c-ret)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170421
[St] Status:MEDLINE
[do] DOI:10.1136/jclinpath-2017-204360


  3 / 2016 MEDLINE  
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[PMID]:27940652
[Au] Autor:Khan F; Hamid A; Fatima B; Hashmi S; Fatimi S
[Ad] Endereço:1 Section of Cardiothoracic Surgery, Department of Surgery, Aga Khan University Hospital, Karachi, Pakistan.
[Ti] Título:Pulmonary hyalinizing granuloma presenting with dysphagia: a rare presentation.
[So] Source:Asian Cardiovasc Thorac Ann;25(1):67-69, 2017 Jan.
[Is] ISSN:1816-5370
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A 25-year-old man presented with a 2-month history of dysphagia and past history of pulmonary and intestinal tuberculosis. A barium swallow showed a point of constriction 42 mm above the gastroesophageal junction. Computed tomography revealed large opacities in bilateral lung fields, encroaching more on the esophagus. The lesion progressively compressed the esophagus as it moved inferiorly. A right posterolateral thoracotomy was performed for sub-anatomical resection of the mass. A biopsy revealed homogenous whirling hyalinized collagen fibers, highly suggestive of pulmonary hyalinizing granuloma, with no evidence of malignancy. Pulmonary hyalinizing granuloma should be considered in the differential diagnosis of longstanding dysphagia.
[Mh] Termos MeSH primário: Transtornos de Deglutição/etiologia
Granuloma do Sistema Respiratório/complicações
Hialina
Pulmão
[Mh] Termos MeSH secundário: Administração Oral
Adulto
Sulfato de Bário/administração & dosagem
Biópsia
Meios de Contraste/administração & dosagem
Deglutição
Transtornos de Deglutição/diagnóstico por imagem
Transtornos de Deglutição/fisiopatologia
Transtornos de Deglutição/cirurgia
Diagnóstico Diferencial
Granuloma do Sistema Respiratório/diagnóstico por imagem
Granuloma do Sistema Respiratório/patologia
Granuloma do Sistema Respiratório/cirurgia
Seres Humanos
Pulmão/diagnóstico por imagem
Pulmão/patologia
Pulmão/cirurgia
Masculino
Valor Preditivo dos Testes
Toracotomia
Tomografia Computadorizada por Raios X
Resultado do Tratamento
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Contrast Media); 25BB7EKE2E (Barium Sulfate)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170213
[Lr] Data última revisão:
170213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161213
[St] Status:MEDLINE
[do] DOI:10.1177/0218492316684983


  4 / 2016 MEDLINE  
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[PMID]:27823834
[Au] Autor:Morimoto S; Hatsuta H; Komiya T; Kanemaru K; Tokumaru AM; Murayama S
[Ad] Endereço:Department of Neuropathology, Tokyo Metropolitan Geriatric Hospital and Institution of Gerontology, Tokyo, Japan; Department of Neurology, Tokyo Metropolitan Geriatric Hospital and Institution of Gerontology, Tokyo, Japan. Electronic address: s.morimoto.tmgh@gmail.com.
[Ti] Título:Simultaneous skin-nerve-muscle biopsy and abnormal mitochondrial inclusions in intranuclear hyaline inclusion body disease.
[So] Source:J Neurol Sci;372:447-449, 2017 Jan 15.
[Is] ISSN:1878-5883
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Mh] Termos MeSH primário: Hialina/metabolismo
Corpos de Inclusão Intranuclear/patologia
Músculos/patologia
Tecido Nervoso/patologia
Pele/inervação
[Mh] Termos MeSH secundário: Idoso
Biópsia
Confusão/complicações
Confusão/diagnóstico
Discalculia/complicações
Discalculia/diagnóstico
Seres Humanos
Imagem por Ressonância Magnética
Masculino
Transtornos da Memória/complicações
Transtornos da Memória/diagnóstico
Mitocôndrias/patologia
Mitocôndrias/ultraestrutura
[Pt] Tipo de publicação:CASE REPORTS; LETTER
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161109
[St] Status:MEDLINE


  5 / 2016 MEDLINE  
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[PMID]:27769871
[Au] Autor:Hsieh MS; Wang H; Lee YH; Ko JY; Chang YL
[Ad] Endereço:Department of Pathology, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei 10002, Taiwan; Graduate Institute of Pathology, College of Medicine, National Taiwan University, Taipei 10002, Taiwan.
[Ti] Título:Reevaluation of MAML2 fusion-negative mucoepidermoid carcinoma: a subgroup being actually hyalinizing clear cell carcinoma of the salivary gland with EWSR1 translocation.
[So] Source:Hum Pathol;61:9-18, 2017 Mar.
[Is] ISSN:1532-8392
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hyalinizing clear cell carcinoma (HCCC) is a rare salivary gland tumor with a specific EWSR1-ATF1 fusion gene and can have mucin production. Mucoepidermoid carcinoma (MEC) with a clear cell component is its morphologic mimic. Using MAML2 fluorescence in situ hybridization (FISH), a total of 49 MEC cases were separated into MAML2 fusion-positive (32 cases) and MAML2 fusion-negative groups (17 cases). This study used EWSR1 FISH to investigate MAML2 fusion-negative cases to identify previously unrecognized HCCC. Among 17 MAML2 fusion-negative cases, 3 had rearrangement of the EWSR1 gene and were reclassified as HCCC. Including 5 previously diagnosed HCCC cases, these 8 HCCC cases had a male-to-female ratio of 1:7, and most (7/8) tumors arose from oral minor salivary glands in the oral cavity (tongue base and palate). EWSR1-ATF1 fusion was confirmed by FISH in all 8 HCCC cases. The histologic features between genetically confirmed HCCC and MEC were compared. HCCC was significantly associated with minor salivary gland involvement, a discrepancy between low-grade cytology and intermediate- to high-grade histology using the MEC grading system, and absence of both epidermoid cells with abundant cytoplasm and goblet cells lining cysts or forming clusters. Clear cells and a hyalinized stroma were not specific for HCCC. HCCC may be erroneously classified as MEC because clear cells may be a minor histologic component and mucin production is not uncommon. Previously diagnosed MEC cases should be reevaluated, especially those arising from minor salivary glands or without MAML2 fusion. Careful histologic evaluation with supporting molecular testing can facilitate pathologic diagnoses.
[Mh] Termos MeSH primário: Biomarcadores Tumorais/genética
Carcinoma Mucoepidermoide/genética
Proteínas de Ligação a DNA/genética
Fusão Gênica
Hialina/química
Proteínas Nucleares/genética
Proteínas de Fusão Oncogênicas/genética
Neoplasias das Glândulas Salivares/genética
Fatores de Transcrição/genética
Translocação Genética
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Biomarcadores Tumorais/análise
Carcinoma Mucoepidermoide/química
Carcinoma Mucoepidermoide/classificação
Carcinoma Mucoepidermoide/patologia
Criança
Feminino
Predisposição Genética para Doença
Seres Humanos
Imuno-Histoquímica
Hibridização in Situ Fluorescente
Masculino
Meia-Idade
Gradação de Tumores
Fenótipo
Valor Preditivo dos Testes
Neoplasias das Glândulas Salivares/química
Neoplasias das Glândulas Salivares/classificação
Neoplasias das Glândulas Salivares/patologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (DNA-Binding Proteins); 0 (EWSR1-ATF1 fusion protein, human); 0 (MAML2 protein, human); 0 (Nuclear Proteins); 0 (Oncogene Proteins, Fusion); 0 (Transcription Factors)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161023
[St] Status:MEDLINE


  6 / 2016 MEDLINE  
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[PMID]:27643581
[Au] Autor:Milutinovic A; Petrovic D; Zorc M; Vraspir Porenta O; Arko M; Pleskovic A; Alibegovic A; Zorc-Pleskovic R
[Ad] Endereço:University of Ljubljana, Faculty of Medicine, Institute of Histology and Embryology, Ljubljana, Slovenia.
[Ti] Título:Mast Cells Might Have a Protective Role against the Development of Calcification and Hyalinisation in Severe Aortic Valve Stenosis.
[So] Source:Folia Biol (Praha);62(4):160-6, 2016.
[Is] ISSN:0015-5500
[Cp] País de publicação:Czech Republic
[La] Idioma:eng
[Ab] Resumo:Aortic valve stenosis is characterized by inflammation and extracellular matrix remodelling. The aim of this study was to analyse the impact of mast cells on the occurrence of histopathological changes of aortic valves in patients with severe grade, non-rheumatic degenerative aortic valve stenosis. Valve specimens were obtained from 38 patients undergoing valve replacement. The role of mast cells was analysed by dividing the specimens into two groups, characterized by the presence (group A, N = 13) or absence of mast cells (group B, N = 25). There were no significant differences in clinical data between the two groups. In group A, T cells and macrophages were present in all aortic valves, as compared to a significantly lower proportion of valves with T cells and macrophages in group B. Valves in group A were less often calcified and hyaline-degenerated than valves in group B. There were no changes in fibrosis between the two groups. We found a positive correlation between the presence of mast cells and macrophages/T cells, a negative correlation between the presence of mast cells and calcification/ hyaline degeneration, and no correlation between the presence of mast cells and fibrosis. There was also a negative correlation between the presence of macrophages/T cells and calcification. The linear regression model identified only the presence of mast cells as an independent negative prediction value for calcification. In conclusion, mast cells might have a protective role against the development of calcification and hyaline degeneration in severe grade, non-rheumatic aortic valve stenosis.
[Mh] Termos MeSH primário: Estenose da Valva Aórtica/patologia
Calcinose/patologia
Hialina/metabolismo
Mastócitos/metabolismo
Substâncias Protetoras/metabolismo
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Valva Aórtica/patologia
Matriz Extracelular/metabolismo
Feminino
Seres Humanos
Inflamação/patologia
Macrófagos/metabolismo
Masculino
Meia-Idade
Linfócitos T/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Protective Agents)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170227
[Lr] Data última revisão:
170227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160920
[St] Status:MEDLINE


  7 / 2016 MEDLINE  
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[PMID]:27634062
[Au] Autor:Saillenfait AM; Marquet F; Sabaté JP; Ndiaye D; Lambert-Xolin AM
[Ad] Endereço:Institut National de Recherche et de Sécurité, Vandoeuvre, France. Electronic address: anne-marie.saillenfait@inrs.fr.
[Ti] Título:4-Week repeated dose oral toxicity study of N-ethyl-2-pyrrolidone in Sprague Dawley rats.
[So] Source:Regul Toxicol Pharmacol;81:275-283, 2016 Nov.
[Is] ISSN:1096-0295
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The solvent N-ethyl-2-pyrrolidone (NEP) was evaluated in a 4-week repeated dose study in rats. NEP diluted in distilled water was orally administered by gavage to male and female Sprague-Dawley rats at doses of 0 (vehicle control), 5, 50, and 250 mg/kg/day for 28 consecutive days. Transient decreases in the body weight and in the body weight gain of the males was observed during the first days of treatment at the 50 and 250 mg/kg/day doses. There was a marked increase in urine volume at the beginning of treatment in males and female rats at doses of 50 and 250 mg/kg/day. No biologically significant differences were observed in hematological and clinical chemistry values in males and females at necropsy. Histological examination revealed an increase in hyaline droplets in the renal tubules of the kidneys and hepatocellular centrilobular hypertrophy in the liver of males at 250 mg/kg/day. Cytochrome P450 concentration in liver microsomes was slightly increased at 250 mg/kg/day in males. The results of this study demonstrate that NEP has mild to no effects at doses up to 250 mg/kg/day when administered orally to rats for 28 days with males being more susceptible than females.
[Mh] Termos MeSH primário: Rim/efeitos dos fármacos
Fígado/efeitos dos fármacos
Pirrolidinonas/toxicidade
[Mh] Termos MeSH secundário: Administração Oral
Animais
Sistema Enzimático do Citocromo P-450/análise
Sistema Enzimático do Citocromo P-450/metabolismo
Relação Dose-Resposta a Droga
Feminino
Hialina/efeitos dos fármacos
Rim/metabolismo
Rim/patologia
Fígado/metabolismo
Fígado/patologia
Masculino
Microssomos Hepáticos/efeitos dos fármacos
Microssomos Hepáticos/enzimologia
Microssomos Hepáticos/metabolismo
Pirrolidinonas/administração & dosagem
Ratos
Ratos Sprague-Dawley
Relação Estrutura-Atividade
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (N-ethyl-2-pyrrolidone); 0 (Pyrrolidinones); 9035-51-2 (Cytochrome P-450 Enzyme System)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170704
[Lr] Data última revisão:
170704
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160917
[St] Status:MEDLINE


  8 / 2016 MEDLINE  
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[PMID]:27614668
[Au] Autor:Prayson RA
[Ad] Endereço:From the Department of Anatomic Pathology, Cleveland Clinic, Cleveland, OH. praysor@ccf.org.
[Ti] Título:Hyaline Protoplasmic Astrocytopathy: A Clinicopathologic Study.
[So] Source:Am J Clin Pathol;146(4):503-9, 2016 Oct.
[Is] ISSN:1943-7722
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: Hyaline protoplasmic astrocytopathy is a rare condition marked by the accumulation of various proteins in the cytoplasm of protoplasmic astrocytes. METHODS: This study retrospectively reviews the clinicopathologic features of 14 patients (nine females; mean age, 9.1 years) with hyaline protoplasmic astrocytopathy. RESULTS: Patients had chronic seizures (mean duration, 101.1 months); mean age at seizure onset was 6.9 months. Three patients had Aicardi syndrome, and one patient had tuberous sclerosis. Associated focal cortical dysplasia was noted in all 14 cases. Patterns of dysplasia observed included International League Against Epilepsy (ILAE) type I Ib (n = 7), ILAE type Ib (n = 4), ILAE type Ic (n = 2), and ILAE type IIa (n = 1). Additional pathologic findings included evidence of contusional damage (n = 5), gray matter nodular heterotopias (n = 3), polymicrogygria (n = 2), hemimegalencephaly (n = 2), and hippocampal sclerosis (ILAE type II, CA1 sclerosis) (n = 1). Postoperative follow-up intervals ranged from two to 93 months (mean, 31.2 months). Seven patients were free of seizures or had only rare disabling seizures (Engel I/II) at most recent follow-up. Two patients had recurrent seizures with no worthwhile improvement (Engel IV) at follow-up. CONCLUSIONS: The current series supports an association of Aicardi syndrome (21% of cases) and focal cortical dysplasia (100% of cases) and hyaline protoplasmic astrocytopathy.
[Mh] Termos MeSH primário: Astrócitos/patologia
Encéfalo/patologia
Epilepsia/patologia
Hialina/metabolismo
[Mh] Termos MeSH secundário: Adolescente
Adulto
Astrócitos/metabolismo
Encéfalo/metabolismo
Criança
Pré-Escolar
Epilepsia/metabolismo
Feminino
Seres Humanos
Masculino
Estudos Retrospectivos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:160912
[St] Status:MEDLINE
[do] DOI:10.1093/ajcp/aqw136


  9 / 2016 MEDLINE  
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[PMID]:27593552
[Au] Autor:Medina EA; Fuehrer NE; Miller FR; Kinney MC; Higgins RA
[Ad] Endereço:Department of Pathology, University of Texas Health Science Center, San Antonio, TX, USA.
[Ti] Título:Dysplastic follicular dendritic cells in hyaline-vascular Castleman disease: a rare occurrence creating diagnostic difficulty.
[So] Source:Pathol Int;66(9):535-9, 2016 Sep.
[Is] ISSN:1440-1827
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:Follicular dendritic cell (FDC) proliferations and dysplastic FDCs can be seen in Hyaline-vascular Castleman disease (HVCD). The association between HVCD and FDC sarcoma is well-documented; dysplastic FDCs may be precursors to FDC sarcoma. Herein, we describe a case of HVCD with strikingly large and dysplastic FDCs, which raised the differential of Hodgkin lymphoma and other neoplasms. Scattered dysplastic FDCs were predominantly in germinal centers and mantle zones, and rarely in interfollicular areas. Although occasional germinal centers contained increased FDCs, no mass forming proliferations were present to suggest FDC sarcoma. Immunostaining demonstrated that the atypical FDCs expressed CD21, clusterin and CXCL13, but not CD23, S100, pankeratin or CD30; they aberrantly expressed epidermal growth factor receptor (EGFR). The present case demonstrates that dysplastic FDCs may be present as isolated cells that require immunophenotyping to distinguish them from malignant entities with similar morphologic features. A variety of FDC markers is required to confirm their origin as the expression of any single marker is not assured, as occurred in this case. Pathologists need be aware of FDC proliferations in HVCD because of their association with FDC sarcoma. Aberrant EGFR expression by dysplastic FDCs may indicate that they are pre-neoplastic and necessitate long-term patient follow-up.
[Mh] Termos MeSH primário: Doença de Castleman/diagnóstico
Doença de Castleman/patologia
Células Dendríticas Foliculares/patologia
[Mh] Termos MeSH secundário: Adulto
Biomarcadores/análise
Diagnóstico Diferencial
Feminino
Doença de Hodgkin/diagnóstico
Seres Humanos
Hialina/metabolismo
Imuno-Histoquímica
[Pt] Tipo de publicação:CASE REPORTS
[Nm] Nome de substância:
0 (Biomarkers)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160906
[St] Status:MEDLINE
[do] DOI:10.1111/pin.12446


  10 / 2016 MEDLINE  
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[PMID]:27526448
[Au] Autor:Linyi C; Xiangli K; Jing X
[Ti] Título:[Effects of in vitro continuous passaging on the phenotype of mouse hyaline chondrocytes and the balance of the extra- cellular matrix].
[So] Source:Hua Xi Kou Qiang Yi Xue Za Zhi;34(3):248-54, 2016 Jun.
[Is] ISSN:1000-1182
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: This study aimed to investigate the effects of in vitro continuous passaging on the morphological phenotype and differentiation characteristics of mouse hyaline chondrocytes, as well as on the balance of the extracellular matrix (ECM). METHODS: Enzymatic digestion was conducted to isolate mouse hyaline chondrocytes, which expanded over five passages in vitro. Hematoxylin-eosin stain was used to show the changes in chondrocyte morphology. Semi-quantitative polymerase chain reaction was performed to analyze the mRNA changes in the marker genes, routine genes, matrix metalloproteinases (MMPs), and tissue inhibitors of MMPs (TIMPs) in chondrocytes. Zymography was carried out to elucidate changes in gelatinase activities. RESULTS: After continuous expansion in vitro, the morphology of round or polygonal chondrocytes changed to elongated and spindled shape. The expression of marker genes significantly decreased (P < 0.05), and it was almost negatively expressed by P5 chondrocytes. By contrast, the down regulation of routine genes was insignificant. The gene expression levels of MMPs and TIMPs both decreased (P < 0.05), but the change in MMP-1 and TIMP-1 was not statistically significant (P > 0.05). Meanwhile, the ratio of MMPs/TIMPs was altered. At the protein level, the activities of gelatinases decreased after passaging, especially for P4 and P5 chondrocytes (P < 0.05). CONCLUSION: Serially passaged chondrocytes dedifferentiated and lost specific phenotypic characteristics during in vitro expansion culture. Simultaneously, the anabolism and catabolism of the cartilage ECM became uncontrollable and led to the imbalance of ECM homeostasis. When hyaline chondrocytes are applied in research on relevant diseases or cartilage tissue engineering, P0-P2 chondrocytes should be used.
[Mh] Termos MeSH primário: Condrócitos/fisiologia
Hialina/fisiologia
[Mh] Termos MeSH secundário: Animais
Cartilagem
Diferenciação Celular
Células Cultivadas
Citoesqueleto
Matriz Extracelular
Gelatinases
Expressão Gênica
Metaloproteinase 1 da Matriz
Metaloproteinases da Matriz
Camundongos
RNA Mensageiro
Engenharia Tecidual
Inibidor Tecidual de Metaloproteinase-1
Inibidores Teciduais de Metaloproteinases
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Messenger); 0 (TIMP1 protein, human); 0 (Tissue Inhibitor of Metalloproteinase-1); 0 (Tissue Inhibitor of Metalloproteinases); EC 3.4.24.- (Gelatinases); EC 3.4.24.- (Matrix Metalloproteinases); EC 3.4.24.7 (MMP1 protein, human); EC 3.4.24.7 (Matrix Metalloproteinase 1)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:160817
[St] Status:MEDLINE



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