Base de dados : MEDLINE
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  1 / 6707 MEDLINE  
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[PMID]:28455220
[Au] Autor:Lee HT; Kim HJ; Kim CS; Gomi K; Taya M; Nomura S; Ahn SH
[Ad] Endereço:Department of Mechanical and Aerospace Engineering, Seoul National University, Seoul 08826, Republic of Korea.
[Ti] Título:Site-specific characterization of beetle horn shell with micromechanical bending test in focused ion beam system.
[So] Source:Acta Biomater;57:395-403, 2017 Jul 15.
[Is] ISSN:1878-7568
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Biological materials are the result of years of evolution and possess a number of efficient features and structures. Researchers have investigated the possibility of designing biomedical structures that take advantage of these structural features. Insect shells, such as beetle shells, are among the most promising types of biological material for biomimetic development. However, due to their intricate geometries and small sizes, it is challenging to measure the mechanical properties of these microscale structures. In this study, we developed an in-situ testing platform for site-specific experiments in a focused ion beam (FIB) system. Multi-axis nano-manipulators and a micro-force sensor were utilized in the testing platform to allow better results in the sample preparation and data acquisition. The entire test protocol, consisting of locating sample, ion beam milling and micro-mechanical bending tests, can be carried out without sample transfer or reattachment. We used our newly devised test platform to evaluate the micromechanical properties and structural features of each separated layer of the beetle horn shell. The Young's modulus of both the exocuticle and endocuticle layers was measured. We carried out a bending test to characterize the layers mechanically. The exocuticle layer bent in a brick-like manner, while the endocuticle layer exhibited a crack blunting effect. STATEMENT OF SIGNIFICANCE: This paper proposed an in-situ manipulation/test method in focused ion beam for characterizing micromechanical properties of beetle horn shell. The challenge in precise and accurate fabrication for the samples with complex geometry was overcome by using nano-manipulators having multi-degree of freedom and a micro-gripper. With the aid of this specially designed test platform, bending tests were carried out on cantilever-shaped samples prepared by focused ion beam milling. Structural differences between exocuticle and endocuticle layers of beetle horn shell were explored and the results provided insight into the structural advantages of each biocomposite structure.
[Mh] Termos MeSH primário: Estruturas Animais/química
Coleópteros/química
Módulo de Elasticidade
Estresse Mecânico
[Mh] Termos MeSH secundário: Animais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE


  2 / 6707 MEDLINE  
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[PMID]:29405690
[Au] Autor:Shchenkov SV
[Ti] Título:[Description of virgulate Cercaria etgesh larva Nov. (Xiphidiocercariae): A new type of virgule organ].
[So] Source:Parazitologiia;51(2):158-64, 2017 Mar-Apr.
[Is] ISSN:0031-1847
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:A new species of virgulate cercaria is described. The morphology and chaetotaxy of cercariae are studied. The new type of virgule organ is described. Every description is illustrated by drawings.
[Mh] Termos MeSH primário: Estruturas Animais/anatomia & histologia
Cercárias/anatomia & histologia
Gastrópodes/parasitologia
Trematódeos/anatomia & histologia
[Mh] Termos MeSH secundário: Estruturas Animais/fisiologia
Animais
Cercárias/fisiologia
Rios/parasitologia
Federação Russa
Trematódeos/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180207
[St] Status:MEDLINE


  3 / 6707 MEDLINE  
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[PMID]:29320528
[Au] Autor:Peterson T; Müller GB
[Ad] Endereço:Department of Theoretical Biology, University of Vienna, Wien, Austria.
[Ti] Título:Developmental finite element analysis of cichlid pharyngeal jaws: Quantifying the generation of a key innovation.
[So] Source:PLoS One;13(1):e0189985, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Advances in imaging and modeling facilitate the calculation of biomechanical forces in biological specimens. These factors play a significant role during ontogenetic development of cichlid pharyngeal jaws, a key innovation responsible for one of the most prolific species diversifications in recent times. MicroCT imaging of radiopaque-stained vertebrate embryos were used to accurately capture the spatial relationships of the pharyngeal jaw apparatus in two cichlid species (Haplochromis elegans and Amatitlania nigrofasciata) for the purpose of creating a time series of developmental stages using finite element models, which can be used to assess the effects of biomechanical forces present in a system at multiple points of its ontogeny. Changes in muscle vector orientations, bite forces, force on the neurocranium where cartilage originates, and stress on upper pharyngeal jaws are analyzed in a comparative context. In addition, microCT scanning revealed the presence of previously unreported cement glands in A. nigrofasciata. The data obtained provide an underrepresented dimension of information on physical forces present in developmental processes and assist in interpreting the role of developmental dynamics in evolution.
[Mh] Termos MeSH primário: Estruturas Animais/anatomia & histologia
Ciclídeos/anatomia & histologia
Estresse Mecânico
[Mh] Termos MeSH secundário: Estruturas Animais/embriologia
Estruturas Animais/crescimento & desenvolvimento
Animais
Evolução Biológica
Região Branquial
Ciclídeos/embriologia
Ciclídeos/crescimento & desenvolvimento
Simulação por Computador
Ingestão de Alimentos/fisiologia
Análise de Elementos Finitos
Mastigação/fisiologia
Modelos Biológicos
Morfogênese
Contração Muscular
Músculos Faríngeos/embriologia
Músculos Faríngeos/crescimento & desenvolvimento
Músculos Faríngeos/fisiologia
Crânio/embriologia
Crânio/crescimento & desenvolvimento
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180111
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189985


  4 / 6707 MEDLINE  
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[PMID]:29089143
[Au] Autor:Tang X; Liang Q; Liu L; Sheng X; Xing J; Zhan W
[Ad] Endereço:Laboratory of Pathology and Immunology of Aquatic Animals, KLM, Ocean University of China, 5 Yushan Road, Qingdao 266003, PR China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, No.1 Wenhai Road, Aoshanwei Town,
[Ti] Título:An optimized double-antibody sandwich ELISA for quantitative detection of WSSV in artificially infected crayfish.
[So] Source:J Virol Methods;251:133-138, 2018 Jan.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Developing a rapid, accurate and quantitative method for detecting white spot syndrome virus (WSSV) is extremely urgent and critical for reducing the risk of white spot disease outbreaks. In the present work, an optimized double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) was developed for quantitative detection of WSSV. The method employed rabbit polyclonal antibodies against WSSV as the capture antibody and previously produced anti-WSSV monoclonal antibodies as the detector antibody. A standard curve of the log concentration of WSSV versus OD value was established, which was linear in the concentration range of 120-7680ng/mL, and the linear regression equation was y=0.166x-0.151. Viral proteins in different tissues of crayfish (Procambarus clarkia) post artificial infection with WSSV were quantitatively measured using the DAS-ELISA. WSSV proliferated quickly within 60h post infection and gradually slowed down afterwards. According to the linear regression relationship, the viral proteins in hemolymph, gut and gonad were firstly able to be quantified at 24h post infection with the concentrations of 186, 158 and 128ng/mL, respectively. These three tissues also contained higher viral proteins than the gill, heart, hepatopancreas and muscle during the entire infection period. The viral protein concentration in gut reached the highest level of 6220ng/mL at 72h post infection. Real time quantitative PCR was also used to detect the dynamic change of viral copies in crayfish hemolymph post WSSV infection, with similar results for both assays. The developed DAS-ELISA could detect WSSV propagation from initial to moribund stage in infected crayfish and demonstrated potential application for diagnosis of WSSV.
[Mh] Termos MeSH primário: Anticorpos Antivirais/imunologia
Astacoidea/virologia
Infecções por Vírus de DNA/veterinária
Ensaio de Imunoadsorção Enzimática/métodos
Carga Viral/métodos
Vírus 1 da Síndrome da Mancha Branca/isolamento & purificação
[Mh] Termos MeSH secundário: Estruturas Animais/virologia
Animais
Infecções por Vírus de DNA/virologia
Coelhos
Fatores de Tempo
Vírus 1 da Síndrome da Mancha Branca/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171102
[St] Status:MEDLINE


  5 / 6707 MEDLINE  
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[PMID]:29261770
[Au] Autor:Wang B; Liu D; Wang C; Wang Q; Zhang H; Liu G; He Q; Zhang L
[Ad] Endereço:Marine Bio-pharmaceutical Institute, Second Military Medical University, Shanghai, China.
[Ti] Título:Tentacle extract from the jellyfish Cyanea capillata increases proliferation and migration of human umbilical vein endothelial cells through the ERK1/2 signaling pathway.
[So] Source:PLoS One;12(12):e0189920, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Wound healing is a complex biological process, and current research finds that jellyfish have a great capacity for promoting growth and healing. However, the underlying mechanisms remain unclear. Thus, this study was conducted to investigate the molecular mechanisms and effects of a tentacle extract (TE) from the jellyfish Cyanea capillata (C. capillata) on cell proliferation and migration in human umbilical vein endothelial cells (HUVECs). First, our results showed that TE at the concentration of 1 µg/ml could promote cell proliferation over various durations, induce a transition of the cells from the G1-phase to the S/G2-phase of the cell cycle, and increase the expression of cell cycle proteins (CyclinB1 and CyclinD1). Second, we found that TE could activate the PI3K/Akt, ERK1/2 and JNK MAPK signaling pathways but not the NF-κB signaling pathway or the apoptosis signaling cascade. Finally, we demonstrated that the TE-induced expression of cell cycle proteins was decreased by ERK1/2 inhibitor PD98059 but not by PI3K inhibitor LY294002 or JNK inhibitor SP600125. Similarly, the TE-enhanced migration ability of HUVECs was also markedly attenuated by PD98059. Taken together, our findings indicate that TE-induced proliferation and migration in HUVECs mainly occurred through the ERK1/2 MAPK signaling pathway. These results are instructively important for further research on the isolation and purification of growth-promoting factors from C. capillata and are hopeful as a means to improve human wound repair in unfavorable conditions.
[Mh] Termos MeSH primário: Estruturas Animais/química
Movimento Celular/efeitos dos fármacos
Células Endoteliais da Veia Umbilical Humana/citologia
Sistema de Sinalização das MAP Quinases/efeitos dos fármacos
Cifozoários/anatomia & histologia
Extratos de Tecidos/farmacologia
[Mh] Termos MeSH secundário: Animais
Ciclo Celular/efeitos dos fármacos
Proteínas de Ciclo Celular/metabolismo
Proliferação Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Ciclinas/metabolismo
Imunofluorescência
Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos
Células Endoteliais da Veia Umbilical Humana/metabolismo
Seres Humanos
NF-kappa B/metabolismo
Fosfatidilinositol 3-Quinases/metabolismo
Fosforilação/efeitos dos fármacos
Inibidores de Proteínas Quinases/farmacologia
Proteínas Proto-Oncogênicas c-akt/metabolismo
Cicatrização/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cell Cycle Proteins); 0 (Cyclins); 0 (NF-kappa B); 0 (Protein Kinase Inhibitors); 0 (Tissue Extracts); EC 2.7.1.- (Phosphatidylinositol 3-Kinases); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189920


  6 / 6707 MEDLINE  
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[PMID]:29183941
[Au] Autor:Abzhanov A
[Ad] Endereço:Department of Life Sciences, Imperial College London, Ascot SL5 7PY, UK a.abzhanov@imperial.ac.uk.
[Ti] Título:The old and new faces of morphology: the legacy of D'Arcy Thompson's 'theory of transformations' and 'laws of growth'.
[So] Source:Development;144(23):4284-4297, 2017 Dec 01.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In 1917, the publication of by D'Arcy Wentworth Thompson challenged both mathematicians and naturalists to think about biological shapes and diversity as more than a confusion of chaotic forms generated at random, but rather as geometric shapes that could be described by principles of physics and mathematics. Thompson's work was based on the ideas of Galileo and Goethe on morphology and of Russell on functionalism, but he was first to postulate that physical forces and internal growth parameters regulate biological forms and could be revealed via geometric transformations in morphological space. Such precise mathematical structure suggested a unifying generative process, as reflected in the title of the book. To Thompson it was growth that could explain the generation of any particular biological form, and changes in ontogeny, rather than natural selection, could then explain the diversity of biological shapes. Whereas adaptationism, widely accepted in evolutionary biology, gives primacy to extrinsic factors in producing morphological variation, Thompson's 'laws of growth' provide intrinsic directives and constraints for the generation of individual shapes, helping to explain the 'profusion of forms, colours, and other modifications' observed in the living world.
[Mh] Termos MeSH primário: Biologia do Desenvolvimento
Crescimento
[Mh] Termos MeSH secundário: Estruturas Animais/anatomia & histologia
Estruturas Animais/crescimento & desenvolvimento
Animais
Evolução Biológica
Aves/anatomia & histologia
Aves/crescimento & desenvolvimento
Biologia do Desenvolvimento/tendências
Modelos Biológicos
Morfogênese
Répteis/anatomia & histologia
Répteis/crescimento & desenvolvimento
Seleção Genética
Crânio/anatomia & histologia
Crânio/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171204
[Lr] Data última revisão:
171204
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE
[do] DOI:10.1242/dev.137505


  7 / 6707 MEDLINE  
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[PMID]:28865344
[Au] Autor:Westover JB; Rigas JD; Van Wettere AJ; Li R; Hickerson BT; Jung KH; Miao J; Reynolds ES; Conrad BL; Nielson S; Furuta Y; Thangamani S; Wang Z; Gowen BB
[Ad] Endereço:Utah State University, 5600 Old Main Hill, Logan, UT 84322, USA.
[Ti] Título:Heartland virus infection in hamsters deficient in type I interferon signaling: Protracted disease course ameliorated by favipiravir.
[So] Source:Virology;511:175-183, 2017 Nov.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Heartland virus (HRTV) is an emerging tick-borne virus (Bunyaviridae, Phlebovirus) that has caused sporadic cases of human disease in several central and mid-eastern states of America. Animal models of HRTV disease are needed to gain insights into viral pathogenesis and advancing antiviral drug development. Presence of clinical disease following HRTV challenge in hamsters deficient in STAT2 function underscores the important role played by type I interferon-induced antiviral responses. However, the recovery of most of the infected animals suggests that other mechanisms to control infection and limit disease offer substantial protection. The most prominent disease sign with HRTV infection in STAT2 knockout hamsters was dramatic weight loss with clinical laboratory and histopathology demonstrating acute inflammation in the spleen, lymph node, liver and lung. Finally, we show that HRTV disease in hamsters can be prevented by the use of favipiravir, a promising broad-spectrum antiviral in clinical development for the treatment of influenza.
[Mh] Termos MeSH primário: Amidas/uso terapêutico
Antivirais/uso terapêutico
Infecções por Bunyaviridae/patologia
Infecções por Bunyaviridae/prevenção & controle
Pirazinas/uso terapêutico
Fator de Transcrição STAT2/deficiência
[Mh] Termos MeSH secundário: Estruturas Animais/patologia
Animais
Quimioprevenção
Cricetinae
Modelos Animais de Doenças
Inflamação/patologia
Interferon Tipo I/imunologia
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amides); 0 (Antiviral Agents); 0 (Interferon Type I); 0 (Pyrazines); 0 (STAT2 Transcription Factor); 0 (Stat2 protein, mouse); EW5GL2X7E0 (favipiravir)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170903
[St] Status:MEDLINE


  8 / 6707 MEDLINE  
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[PMID]:28854252
[Au] Autor:Solazzo C; Fitzhugh W; Kaplan S; Potter C; Dyer JM
[Ad] Endereço:Museum Conservation Institute, Museum Support Center, Smithsonian Institution, Suitland, Maryland, United States of America.
[Ti] Título:Molecular markers in keratins from Mysticeti whales for species identification of baleen in museum and archaeological collections.
[So] Source:PLoS One;12(8):e0183053, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Baleen has been harvested by indigenous people for thousands of years, as well as collected by whalers as an additional product of commercial whaling in modern times. Baleen refers to the food-filtering system of Mysticeti whales; a full baleen rack consists of dozens of plates of a tough and flexible keratinous material that terminate in bristles. Due to its properties, baleen was a valuable raw material used in a wide range of artefacts, from implements to clothing. Baleen is not widely used today, however, analyses of this biomolecular tissue have the potential to contribute to conservation efforts, studies of genetic diversity and a better understanding of the exploitation and use of Mysticeti whales in past and recent times. Fortunately, baleen is present in abundance in museum natural history collections. However, it is often difficult or impossible to make a species identification of manufactured or old baleen. Here, we propose a new tool for biomolecular identification of baleen based on its main structural component alpha-keratin (the same protein that makes up hair and fingernails). With the exception of minke whales, alpha-keratin sequences are not yet known for baleen whales. We therefore used peptide mass fingerprinting to determine peptidic profiles in well documented baleen and evaluated the possibility of using this technique to differentiate species in baleen samples that are not adequately identified or are unidentified. We examined baleen from ten different species of whales and determined molecular markers for each species, including species-specific markers. In the case of the Bryde's whales, differences between specimens suggest distinct species or sub-species, consistent with the complex phylogeny of the species. Finally, the methodology was applied to 29 fragments of baleen excavated from archaeological sites in Labrador, Canada (representing 1500 years of whale use by prehistoric people), demonstrating a dominance of bowhead whale (Balaena mysticetus) in the archaeological assemblage and the successful application of the peptide mass fingerprinting technique to identify the species of whale in unidentified and partially degraded samples.
[Mh] Termos MeSH primário: Estruturas Animais/química
Baleia Franca/classificação
Queratinas/isolamento & purificação
Mapeamento de Peptídeos/métodos
Filogenia
[Mh] Termos MeSH secundário: Estruturas Animais/anatomia & histologia
Animais
Arqueologia/instrumentação
Arqueologia/métodos
Biomarcadores
Baleia Franca/anatomia & histologia
Canadá
Queratinas/classificação
Espectrometria de Massas
Museus
Nova Zelândia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 68238-35-7 (Keratins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170831
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183053


  9 / 6707 MEDLINE  
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Burger, Eva
Texto completo
[PMID]:28854184
[Au] Autor:Della Terra PP; Rodrigues AM; Fernandes GF; Nishikaku AS; Burger E; de Camargo ZP
[Ad] Endereço:Department of Medicine, Discipline of Infectious Diseases, Federal University of São Paulo (UNIFESP), São Paulo, Brazil.
[Ti] Título:Exploring virulence and immunogenicity in the emerging pathogen Sporothrix brasiliensis.
[So] Source:PLoS Negl Trop Dis;11(8):e0005903, 2017 Aug.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sporotrichosis is a polymorphic chronic infection of humans and animals classically acquired after traumatic inoculation with soil and plant material contaminated with Sporothrix spp. propagules. An alternative and successful route of transmission is bites and scratches from diseased cats, through which Sporothrix yeasts are inoculated into mammalian tissue. The development of a murine model of subcutaneous sporotrichosis mimicking the alternative route of transmission is essential to understanding disease pathogenesis and the development of novel therapeutic strategies. To explore the impact of horizontal transmission in animals (e.g., cat-cat) and zoonotic transmission on Sporothrix fitness, the left hind footpads of BALB/c mice were inoculated with 5×106 yeasts (n = 11 S. brasiliensis, n = 2 S. schenckii, or n = 1 S. globosa). Twenty days post-infection, our model reproduced both the pathophysiology and symptomology of sporotrichosis with suppurating subcutaneous nodules that progressed proximally along lymphatic channels. Across the main pathogenic members of the S. schenckii clade, S. brasiliensis was usually more virulent than S. schenckii and S. globosa. However, the virulence in S. brasiliensis was strain-dependent, and we demonstrated that highly virulent isolates disseminate from the left hind footpad to the liver, spleen, kidneys, lungs, heart, and brain of infected animals, inducing significant and chronic weight loss (losing up to 15% of their body weight). The weight loss correlated with host death between 2 and 16 weeks post-infection. Histopathological features included necrosis, suppurative inflammation, and polymorphonuclear and mononuclear inflammatory infiltrates. Immunoblot using specific antisera and homologous exoantigen investigated the humoral response. Antigenic profiles were isolate-specific, supporting the hypothesis that different Sporothrix species can elicit a heterogeneous humoral response over time, but cross reaction was observed between S. brasiliensis and S. schenckii proteomes. Despite great diversity in the immunoblot profiles, antibodies were mainly derived against 3-carboxymuconate cyclase, a glycoprotein oscillating between 60 and 70 kDa (gp60-gp70) and a 100-kDa molecule in nearly 100% of the assays. Thus, our data broaden the current view of virulence and immunogenicity in the Sporothrix-sporotrichosis system, substantially expanding the possibilities for comparative genomic with isolates bearing divergent virulence traits and helping uncover the molecular mechanisms and evolutionary pressures underpinning the emergence of Sporothrix virulence.
[Mh] Termos MeSH primário: Sporothrix/imunologia
Sporothrix/patogenicidade
Esporotricose/imunologia
Esporotricose/patologia
[Mh] Termos MeSH secundário: Estruturas Animais/microbiologia
Estruturas Animais/patologia
Animais
Anticorpos Antifúngicos/sangue
Antígenos de Fungos/imunologia
Peso Corporal
Modelos Animais de Doenças
Histocitoquímica
Immunoblotting
Camundongos Endogâmicos BALB C
Análise de Sobrevida
Fatores de Tempo
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Fungal); 0 (Antigens, Fungal)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170917
[Lr] Data última revisão:
170917
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170831
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005903


  10 / 6707 MEDLINE  
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[PMID]:28850622
[Au] Autor:Aftab SMA; Ahmad KA
[Ad] Endereço:Dept of Aerospace Engineering, Universiti Putra Malaysia, Selangor, 43400, Malaysia.
[Ti] Título:CFD study on NACA 4415 airfoil implementing spherical and sinusoidal Tubercle Leading Edge.
[So] Source:PLoS One;12(8):e0183456, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Humpback whale tubercles have been studied for more than a decade. Tubercle Leading Edge (TLE) effectively reduces the separation bubble size and helps in delaying stall. They are very effective in case of low Reynolds number flows. The current Computational Fluid Dynamics (CFD) study is on NACA 4415 airfoil, at a Reynolds number 120,000. Two TLE shapes are tested on NACA 4415 airfoil. The tubercle designs implemented on the airfoil are sinusoidal and spherical. A parametric study is also carried out considering three amplitudes (0.025c, 0.05c and 0.075c), the wavelength (0.25c) is fixed. Structured mesh is utilized to generate grid and Transition SST turbulence model is used to capture the flow physics. Results clearly show spherical tubercles outperform sinusoidal tubercles. Furthermore experimental study considering spherical TLE is carried out at Reynolds number 200,000. The experimental results show that spherical TLE improve performance compared to clean airfoil.
[Mh] Termos MeSH primário: Estruturas Animais/anatomia & histologia
Simulação por Computador
Jubarte
Modelos Biológicos
[Mh] Termos MeSH secundário: Animais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171121
[Lr] Data última revisão:
171121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170830
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183456



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