Base de dados : MEDLINE
Pesquisa : A13.078 [Categoria DeCS]
Referências encontradas : 2 [refinar]
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[PMID]:29205973
[Au] Autor:Xia X; Ding M
[Ad] Endereço:School of Forensic Medicine, China Medical University, Shenyang 110000, China.
[Ti] Título:[Application of COâ…  Gene Mini-barcoding in Species Identification of Hair].
[So] Source:Fa Yi Xue Za Zhi;32(6):441-443, 2016 Dec.
[Is] ISSN:1004-5619
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVES: To identify the species of mammalian hair using COⅠ gene mini-barcoding technology. METHODS: A pair of universal primers for mammalian COⅠ gene mini-barcoding were designed. The hair DNA samples of experimental animals from 11 species in 5 orders, mammalia was amplified by PCR technology with universal primers, and the PCR products were sequenced by bi-directional DNA and after the sequences splicing the results were deposited into the BOLD database to perform the homology comparison. RESULTS: The DNA of hair from all experiment animal species could be totally amplified by the mini-barcoding universal primers designed in this study. The length of amplified fragment was 147 bp. The result of homology comparison in the database showed that the closest matching species were consistent with the experiment animal species. In addition to the matching degree of (98.99%), all homology matching degree of the other experiment animals were 100%, and the intraspecific genetic distance of was 1%. The interspecific genetic distance was ten times more than the intraspecific genetic distance which could be used for species identification. CONCLUSIONS: The COⅠ gene mini-barcode technology is established and can provide fast and accurate species identification for hair of mammals.
[Mh] Termos MeSH primário: Pelo Animal
Código de Barras de DNA Taxonômico
Cabelo
[Mh] Termos MeSH secundário: Animais
Primers do DNA
Reação em Cadeia da Polimerase
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171206
[St] Status:MEDLINE
[do] DOI:10.3969/j.issn.1004-5619.2016.06.012


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[PMID]:29054756
[Au] Autor:Zhu YB; Wang ZY; Yin RH; Jiao Q; Zhao SJ; Cong YY; Xue HL; Guo D; Wang SQ; Zhu YX; Bai WL
[Ad] Endereço:College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, PR China.
[Ti] Título:A lncRNA-H19 transcript from secondary hair follicle of Liaoning cashmere goat: Identification, regulatory network and expression regulated potentially by its promoter methylation.
[So] Source:Gene;641:78-85, 2018 Jan 30.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The H19 transcript (imprinted maternally expressed transcript) is well-known as long noncoding RNA (lncRNA), and it is thought to be associated with the inductive capacity of dermal papilla cells for hair-follicle reconstruction. In this study, we isolated and characterized a lncRNA-H19 transcript from the secondary hair follicle of Liaoning cashmere goat. Also, we investigated its transcriptional pattern and methylation status of H19 gene in secondary hair follicle of this breed during different stages of hair follicle cycle. Nucleotide composition analysis indicated that guanine (G) and cytosine (C) are the dominant nucleotides in the lncRNA-H19 transcript of Liaoning cashmere goat with the highest frequency distribution (11.25%) of GG nucleotide pair. The regulatory network showed that lncRNA-H19 transcript appears to have remarkably diverse regulatory relationships with its related miRNAs and the potential target genes. In secondary hair follicle, the relative expression of lncRNA-H19 transcript at the anagen phase is significantly higher than that at both telogen and catagen phases suggesting that lncRNA-H19 transcript might play essential roles in the formation and growth of cashmere fiber of goat. Methylation analysis indicated that the methylation of the promoter region of H19 gene most likely participates in its transcriptional suppression in secondary hair follicle of Liaoning cashmere goat.
[Mh] Termos MeSH primário: Pelo Animal/citologia
Metilação de DNA/genética
Cabras/genética
Regiões Promotoras Genéticas/genética
RNA Longo não Codificante/genética
[Mh] Termos MeSH secundário: Animais
Composição de Bases/genética
Sequência de Bases
Regulação da Expressão Gênica/genética
Folículo Piloso/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (H19 long non-coding RNA); 0 (RNA, Long Noncoding)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171022
[St] Status:MEDLINE



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