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[PMID]: | 29198037 |
[Au] Autor: | Hu X; Chen S; Jia C; Xue S; Dou C; Dai Z; Xu H; Sun Z; Geng T; Cui H |
[Ad] Endereço: | Institute of Epigenetics and Epigenomics, College of Animal Science and Technology, Yangzhou University, 48 East Wenhui Road, Yangzhou, 225009, Jiangsu, China. |
[Ti] Título: | Gene expression profile and long non-coding RNA analysis, using RNA-Seq, in chicken embryonic fibroblast cells infected by avian leukosis virus J. |
[So] Source: | Arch Virol;163(3):639-647, 2018 Mar. | [Is] ISSN: | 1432-8798 |
[Cp] País de publicação: | Austria |
[La] Idioma: | eng |
[Ab] Resumo: | Avian leukosis virus J (ALVJ) infection induces hematopoietic malignancy in myeloid leukemia and hemangioma in chickens. However, little is known about the mechanisms underpinning the unique pathogenesis of ALVJ. In this study, we investigated the gene expression profiles of ALVJ-infected chicken cells and performed a comprehensive analysis of the long non-coding RNAs (lncRNAs) in CEF cells using RNA-Seq. As a result, 36 differentially expressed lncRNAs and 91 genes (FC > 2 and q-values < 0.05) were identified. Bioinformatics analysis revealed that these differentially expressed genes are involved in the innate immune response. Target prediction analysis revealed that these lncRNAs may act in cis or trans and affect the expression of genes which are involved in the anti-viral innate immune responses. Toll-like receptor, RIG-I receptor, NOD-like receptor and JAK-STAT signaling pathways were enriched. Notably, the induced expression of innate immunity genes, including B2M, DHX58, IFI27L2, IFIH1, IRF10, ISG12(2), MX, OAS*A, RSAD2, STAT1, TLR3, IL4I1, and IRF1 (FC > 2 and correlation > 0.95), were highly correlated with the upregulation of several lncRNAs, including MG066618, MG066617, MG066601, MG066629, MG066609 and MG066616. These findings identify the expression profile of lncRNAs in chicken CEF cells infected by ALVJ virus and provide new insights into the molecular mechanisms of ALVJ infection. |
[Mh] Termos MeSH primário: |
Vírus da Leucose Aviária/genética Fibroblastos/virologia Interações Hospedeiro-Patógeno RNA Longo não Codificante/genética Transcriptoma/imunologia
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[Mh] Termos MeSH secundário: |
Animais Vírus da Leucose Aviária/crescimento & desenvolvimento Vírus da Leucose Aviária/imunologia Linhagem Celular Embrião de Galinha Biologia Computacional Proteína DEAD-box 58/genética Proteína DEAD-box 58/imunologia Fibroblastos/imunologia Perfilação da Expressão Gênica Regulação da Expressão Gênica Imunidade Inata Janus Quinase 1/genética Janus Quinase 1/imunologia Proteínas NLR/genética Proteínas NLR/imunologia RNA Longo não Codificante/imunologia Fatores de Transcrição STAT/genética Fatores de Transcrição STAT/imunologia Análise de Sequência de RNA Transdução de Sinais Receptores Toll-Like/genética Receptores Toll-Like/imunologia
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (NLR Proteins); 0 (RNA, Long Noncoding); 0 (STAT Transcription Factors); 0 (Toll-Like Receptors); EC 2.7.10.2 (Janus Kinase 1); EC 3.6.4.13 (DEAD Box Protein 58) |
[Em] Mês de entrada: | 1802 |
[Cu] Atualização por classe: | 180220 |
[Lr] Data última revisão:
| 180220 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 171204 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1007/s00705-017-3659-8 |
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