Base de dados : MEDLINE
Pesquisa : A13.395 [Categoria DeCS]
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[PMID]:29246992
[Au] Autor:Berner D
[Ad] Endereço:Department of Clinical Science & Services, Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, Hertfordshire AL9 7TA, UK.
[Ti] Título:Diagnostic imaging of tendinopathies of the superficial flexor tendon in horses.
[So] Source:Vet Rec;181(24):652-654, 2017 12 16.
[Is] ISSN:2042-7670
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Tendinopatia
Tendões
[Mh] Termos MeSH secundário: Animais
Diagnóstico por Imagem
Membro Anterior
Doenças dos Cavalos
Cavalos
Traumatismos dos Tendões/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE; COMMENT
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171217
[St] Status:MEDLINE
[do] DOI:10.1136/vr.j5746


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[PMID]:29037663
[Au] Autor:Blackwell AA; Widick WL; Cheatwood JL; Whishaw IQ; Wallace DG
[Ad] Endereço:Psychology Department, Northern Illinois University, De Kalb, Illinois, USA.
[Ti] Título:Unilateral forelimb sensorimotor cortex devascularization disrupts the topographic and kinematic characteristics of hand movements while string-pulling for food in the rat.
[So] Source:Behav Brain Res;338:88-100, 2018 Feb 15.
[Is] ISSN:1872-7549
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:String-pulling by the rat is a bimanual act, in which an upright animal retrieves a piece of food attached to the end of the string by downward hand-over-hand movements. The present study compared the movements of string-pulling, using topographic and kinematic measures of hand movement, in control rats and rats with unilateral sensorimotor motor cortex lesion produced by removal of the pia matter. In the first week following devascularization, the rhythmicity and accuracy of string-pulling movements decomposed; however, thereafter the rhythm of bilateral alternation was restored. Over 70days of testing, distance traveled decreased for both hands in the control and lesion groups, suggesting that both groups displayed an increase in string-pulling efficiency. Nevertheless, the lesion group exhibited more missed string contacts with the (contralateral-to-lesion) hand and more grasps in which the string was hooked between the digits with both hands. In addition, an increase in mouth grasps was observed in the lesion group. Motion capture analyses revealed that the lesion group exhibited longer reach and withdraw movements and these movements were longer for the ipsilateral-to-lesion vs contralateral-to-lesion hand. Thus, although rhythmicity of string-pulling behavior recovers after sensorimotor cortex devascularization, the contralateral-to-lesion hand contributed less to string pulling and requires mouth grasps to stabilize the string for grasping. The results are discussed in relation to contemporary theories of the contributions of the forelimb motor cortex to skilled movement and the potential use of string-pulling as a therapy for brain injury.
[Mh] Termos MeSH primário: Membro Anterior/fisiopatologia
Lateralidade Funcional/fisiologia
Movimento/fisiologia
Desempenho Psicomotor/fisiologia
Córtex Sensório-Motor/irrigação sanguínea
[Mh] Termos MeSH secundário: Animais
Fenômenos Biomecânicos/fisiologia
Masculino
Ratos
Ratos Long-Evans
Recuperação de Função Fisiológica/fisiologia
Córtex Sensório-Motor/fisiopatologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171018
[St] Status:MEDLINE


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[PMID]:28461107
[Au] Autor:Gertel S; Mahagna H; Karmon G; Watad A; Amital H
[Ad] Endereço:Zabludowicz Center For Autoimmune Diseases, Sheba Medical Center, Tel-Hashomer 5262100, Israel; Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv 6997801, Israel. Electronic address: smadar.gertel@sheba.health.gov.il.
[Ti] Título:Tofacitinib attenuates arthritis manifestations and reduces the pathogenic CD4 T cells in adjuvant arthritis rats.
[So] Source:Clin Immunol;184:77-81, 2017 11.
[Is] ISSN:1521-7035
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Rheumatoid arthritis (RA) is an autoimmune disease characterized by pronounced inflammation and leukocyte infiltration in affected joints. Tofacitinib is new agent, a selective inhibitor of Janus kinase (JAK) signaling pathways mediated by JAK1 and JAK3 and inhibits the key transcription factors STAT1 and STAT3. We investigated the action mechanisms of tofacitinib in rats with adjuvant-induced-arthritis (AIA). AIA-rats were treated orally with tofacitinib or with methotrexate. Arthritis severity and serum C-reactive protein (CRP) levels were evaluated, splenic cells were examined by flow cytometry and cytokines were analyzed by real-time PCR. Tofacitinib markedly reduced the clinical status of treated rats in comparison to control group. Reduced joints inflammation and down-regulated serum CRP levels reflected the clinical manifestations of the treated rats. Tofacitinib down-regulated significantly the frequency of CD4 IFN-γ T cells and reduced IL-1ß mRNA expression levels in the spleen of the treated rats. These results show that tofacitinib attenuated arthritis severity, modified splenic populations and cytokine imbalance.
[Mh] Termos MeSH primário: Artrite Experimental/imunologia
Artrite Reumatoide/imunologia
Linfócitos T CD4-Positivos/efeitos dos fármacos
Articulações do Pé/efeitos dos fármacos
Piperidinas/farmacologia
Inibidores de Proteínas Quinases/farmacologia
Pirimidinas/farmacologia
Pirróis/farmacologia
[Mh] Termos MeSH secundário: Animais
Antirreumáticos/farmacologia
Artrite Experimental/fisiopatologia
Artrite Reumatoide/fisiopatologia
Proteína C-Reativa/efeitos dos fármacos
Proteína C-Reativa/imunologia
Linfócitos T CD4-Positivos/imunologia

Articulações do Pé/patologia
Membro Anterior
Membro Posterior
Interferon gama/imunologia
Interleucina-1beta/efeitos dos fármacos
Interleucina-1beta/genética
Metotrexato/farmacologia
RNA Mensageiro/efeitos dos fármacos
RNA Mensageiro/metabolismo
Ratos
Reação em Cadeia da Polimerase em Tempo Real
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Baço/citologia
Baço/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antirheumatic Agents); 0 (IL1B protein, rat); 0 (Interleukin-1beta); 0 (Piperidines); 0 (Protein Kinase Inhibitors); 0 (Pyrimidines); 0 (Pyrroles); 0 (RNA, Messenger); 82115-62-6 (Interferon-gamma); 87LA6FU830 (tofacitinib); 9007-41-4 (C-Reactive Protein); YL5FZ2Y5U1 (Methotrexate)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE


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[PMID]:29176874
[Au] Autor:Reyes-Corona D; Vázquez-Hernández N; Escobedo L; Orozco-Barrios CE; Ayala-Davila J; Moreno MG; Amaro-Lara ME; Flores-Martinez YM; Espadas-Alvarez AJ; Fernandez-Parrilla MA; Gonzalez-Barrios JA; Gutierrez-Castillo ME; González-Burgos I; Martinez-Fong D
[Ad] Endereço:Departamento de Fisiología, Biofísica y Neurociencias, Centro de Investigación y de Estudios Avanzados, Ciudad de México, México.
[Ti] Título:Neurturin overexpression in dopaminergic neurons induces presynaptic and postsynaptic structural changes in rats with chronic 6-hydroxydopamine lesion.
[So] Source:PLoS One;12(11):e0188239, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The structural effect of neurturin (NRTN) on the nigrostriatal dopaminergic system in animals remains unknown, although NRTN has been shown to be effective in Parkinson's disease animal models. Herein, we aimed to demonstrate that NRTN overexpression in dopaminergic neurons stimulates both neurite outgrowths in the nigrostriatal pathway and striatal dendritic spines in aging rats with chronic 6-hydroxydopamine (6-OHDA) lesion. At week 12 after lesion, pTracer-mNRTN-His or pGreenLantern-1 plasmids were intranigrally transfected using the NTS-polyplex nanoparticles system. We showed that the transgenic expression in dopaminergic neurons remained until the end of the study (12 weeks). Only animals expressing NRTN-His showed recovery of tyrosine hydroxylase (TH)+ cells (28 ± 2%), their neurites (32 ± 2%) and the neuron-specific cytoskeletal marker ß-III-tubulin in the substantia nigra; striatal TH(+) fibers were also recovered (52 ± 3%), when compared to the healthy condition. Neurotensin receptor type 1 levels were also significantly recovered in the substantia nigra and striatum. Dopamine recovery was 70 ± 4% in the striatum and complete in the substantia nigra. The number of dendritic spines of striatal medium spiny neurons was also significantly increased, but the recovery was not complete. Drug-activated circling behavior decreased by 73 ± 2% (methamphetamine) and 89 ± 1% (apomorphine). Similar decrease was observed in the spontaneous motor behavior. Our results demonstrate that NRTN causes presynaptic and postsynaptic restoration of the nigrostriatal dopaminergic system after a 6-OHDA-induced chronic lesion. However, those improvements did not reach the healthy condition, suggesting that NRTN exerts lesser neurotrophic effects than other neurotrophic approaches.
[Mh] Termos MeSH primário: Neurônios Dopaminérgicos/metabolismo
Neurturina/metabolismo
Terminações Pré-Sinápticas/metabolismo
[Mh] Termos MeSH secundário: Animais
Corpo Estriado/metabolismo
Corpo Estriado/patologia
Citoesqueleto/metabolismo
Espinhas Dendríticas/metabolismo
Dopamina/metabolismo
Ensaio de Imunoadsorção Enzimática
Membro Anterior/fisiologia
Masculino
Camundongos
Neuritos/metabolismo
Oxidopamina
Ratos Wistar
Receptores de Neurotensina/metabolismo
Substância Negra/metabolismo
Substância Negra/patologia
Transfecção
Vibrissas/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Neurturin); 0 (Receptors, Neurotensin); 0 (neurotensin type 1 receptor); 8HW4YBZ748 (Oxidopamine); VTD58H1Z2X (Dopamine)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171219
[Lr] Data última revisão:
171219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188239


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[PMID]:28743862
[Au] Autor:Farlie PG; Davidson NM; Baker NL; Raabus M; Roeszler KN; Hirst C; Major A; Mariette MM; Lambert DM; Oshlack A; Smith CA
[Ad] Endereço:Murdoch Children's Research Institute, Royal Children's Hospital, Parkville, VIC, 3052, Australia. peter.farlie@mcri.edu.au.
[Ti] Título:Co-option of the cardiac transcription factor Nkx2.5 during development of the emu wing.
[So] Source:Nat Commun;8(1):132, 2017 07 25.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The ratites are a distinctive clade of flightless birds, typified by the emu and ostrich that have acquired a range of unique anatomical characteristics since diverging from basal Aves at least 100 million years ago. The emu possesses a vestigial wing with a single digit and greatly reduced forelimb musculature. However, the embryological basis of wing reduction and other anatomical changes associated with loss of flight are unclear. Here we report a previously unknown co-option of the cardiac transcription factor Nkx2.5 to the forelimb in the emu embryo, but not in ostrich, or chicken and zebra finch, which have fully developed wings. Nkx2.5 is expressed in emu limb bud mesenchyme and maturing wing muscle, and mis-expression of Nkx2.5 throughout the limb bud in chick results in wing reductions. We propose that Nkx2.5 functions to inhibit early limb bud expansion and later muscle growth during development of the vestigial emu wing.The transcription factor Nkx2.5 is essential for heart development. Here, the authors identify a previously unknown expression domain for Nkx2.5 in the emu wing and explore its role in diminished wing bud development in the flightless emu, compared with three other birds that have functional wings.
[Mh] Termos MeSH primário: Proteínas Aviárias/genética
Proteína Homeobox Nkx-2.5/genética
Fatores de Transcrição/genética
Asas de Animais/metabolismo
[Mh] Termos MeSH secundário: Animais
Proteínas Aviárias/metabolismo
Dromaiidae
Membro Anterior/embriologia
Membro Anterior/metabolismo
Perfilação da Expressão Gênica/métodos
Regulação da Expressão Gênica no Desenvolvimento
Hibridização In Situ
Botões de Extremidades/embriologia
Botões de Extremidades/metabolismo
Mesoderma/embriologia
Mesoderma/metabolismo
Músculo Esquelético/embriologia
Músculo Esquelético/metabolismo
Miocárdio/metabolismo
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Asas de Animais/embriologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Avian Proteins); 0 (Homeobox Protein Nkx-2.5); 0 (Transcription Factors)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-00112-7


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[PMID]:28460426
[Au] Autor:Schoonover MJ; Moser DK; Young JM; Payton ME; Holbrook TC
[Ad] Endereço:Department of Veterinary Clinical Sciences, Oklahoma State University, Stillwater, Oklahoma.
[Ti] Título:Effects of tourniquet number and exsanguination on amikacin concentrations in the radiocarpal and distal interphalangeal joints after low volume intravenous regional limb perfusion in horses.
[So] Source:Vet Surg;46(5):675-682, 2017 Jul.
[Is] ISSN:1532-950X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To determine the influence of a dual tourniquet technique and limb exsanguination on amikacin concentrations in the synovial fluid of the radiocarpal joint (RCJ) and distal interphalangeal joint (DIPJ) after low volume, cephalic intravenous regional limb perfusion (IVRLP). STUDY DESIGN: Randomized cross-over design. ANIMALS: Six healthy adult horses. METHODS: One gram of amikacin in 6 mL of 0.9% NaCl was infused via cephalic IVRLP in 6 standing, sedated horses using 4 techniques: proximal pneumatic tourniquet (P), proximal pneumatic tourniquet with exsanguination (PE), proximal pneumatic and distal Esmarch tourniquet (PD), and proximal pneumatic with distal Esmarch tourniquet and exsanguination (PDE). Amikacin concentrations were measured in RCJ and DIPJ synovial fluid samples, collected just before perfusion (time 0), and at 15 and 30 minutes (before tourniquet release) after perfusion. RESULTS: Synovial fluid amikacin concentrations achieved in the RCJ were higher with techniques PD and PDE than those achieved with techniques P and PE 15 and 30 minutes after perfusion (P < .0001). Synovial fluid amikacin concentrations in the DIPJ were higher with techniques P and PE than those achieved with techniques PD and PDE at 15 minutes (P = .0002) and were higher than technique PDE at 30 minutes after perfusion (P < .0001). CONCLUSION: Low volume (10 mL) cephalic IVRLP should be combined with the placement of 2 tourniquets (proximal and distal to the carpus) to achieve therapeutic amikacin concentrations in the RCJ. Exsanguination prior to low volume IVRLP does not alter synovial fluid amikacin concentrations.
[Mh] Termos MeSH primário: Amicacina/química
Amicacina/farmacocinética
Antibacterianos/farmacocinética
Cavalos
Líquido Sinovial/química
Torniquetes/veterinária
[Mh] Termos MeSH secundário: Amicacina/administração & dosagem
Animais
Antibacterianos/administração & dosagem
Estudos Cross-Over
Membro Anterior
Perfusão
Procedimentos Cirúrgicos Vasculares
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 84319SGC3C (Amikacin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE
[do] DOI:10.1111/vsu.12662


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[PMID]:28942925
[Au] Autor:Wang X; Liu Y; Li X; Zhang Z; Yang H; Zhang Y; Williams PR; Alwahab NSA; Kapur K; Yu B; Zhang Y; Chen M; Ding H; Gerfen CR; Wang KH; He Z
[Ad] Endereço:F.M. Kirby Neurobiology Center, Boston Children's Hospital and Department of Neurology, Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA.
[Ti] Título:Deconstruction of Corticospinal Circuits for Goal-Directed Motor Skills.
[So] Source:Cell;171(2):440-455.e14, 2017 Oct 05.
[Is] ISSN:1097-4172
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Corticospinal neurons (CSNs) represent the direct cortical outputs to the spinal cord and play important roles in motor control across different species. However, their organizational principle remains unclear. By using a retrograde labeling system, we defined the requirement of CSNs in the execution of a skilled forelimb food-pellet retrieval task in mice. In vivo imaging of CSN activity during performance revealed the sequential activation of topographically ordered functional ensembles with moderate local mixing. Region-specific manipulations indicate that CSNs from caudal or rostral forelimb area control reaching or grasping, respectively, and both are required in the transitional pronation step. These region-specific CSNs terminate in different spinal levels and locations, therefore preferentially connecting with the premotor neurons of muscles engaged in different steps of the task. Together, our findings suggest that spatially defined groups of CSNs encode different movement modules, providing a logic for parallel-ordered corticospinal circuits to orchestrate multistep motor skills.
[Mh] Termos MeSH primário: Medula Cervical/fisiologia
Destreza Motora
Vias Neurais
[Mh] Termos MeSH secundário: Animais
Cálcio/análise
Córtex Cerebral/citologia
Córtex Cerebral/fisiologia
Medula Cervical/citologia
Membro Anterior/fisiologia
Articulações/fisiologia
Camundongos
Camundongos Endogâmicos C57BL
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
SY7Q814VUP (Calcium)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171111
[Lr] Data última revisão:
171111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170926
[St] Status:MEDLINE


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[PMID]:28807899
[Au] Autor:Nemec S; Luxey M; Jain D; Huang Sung A; Pastinen T; Drouin J
[Ad] Endereço:Institut de Recherches Cliniques de Montréal, Montréal, QC, H2W 1R7 Canada.
[Ti] Título: directly modulates the core limb development program to implement hindlimb identity.
[So] Source:Development;144(18):3325-3335, 2017 09 15.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Forelimbs (FLs) and hindlimbs (HLs) develop complex musculoskeletal structures that rely on the deployment of a conserved developmental program. , a transcription factor gene with expression restricted to HL and absent from FL, plays an important role in generating HL features. The genomic mechanisms by which effects HL identity remain poorly understood. Here, we use expression profiling and analysis of direct Pitx1 targets to characterize the HL- and FL-restricted genetic programs in mouse and situate the -dependent gene network within the context of limb-specific gene regulation. We show that is a crucial component of a narrow network of HL-restricted regulators, acting on a developmental program that is shared between FL and HL. Pitx1 targets sites that are in a similar chromatin state in FL and HL and controls expression of patterning genes as well as the chondrogenic program, consistent with impaired chondrogenesis in HL. These findings support a model in which multifactorial actions of a limited number of HL regulators redirect the generic limb development program in order to generate the unique structural features of the limb.
[Mh] Termos MeSH primário: Membro Posterior/embriologia
Membro Posterior/metabolismo
Organogênese
Fatores de Transcrição Box Pareados/metabolismo
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Condrogênese/genética
Embrião de Mamíferos/metabolismo
Elementos Facilitadores Genéticos/genética
Epigênese Genética
Membro Anterior/embriologia
Membro Anterior/metabolismo
Regulação da Expressão Gênica no Desenvolvimento
Redes Reguladoras de Genes
Loci Gênicos
Genoma
Proteínas de Homeodomínio/metabolismo
Camundongos
Organogênese/genética
Fatores de Transcrição SOX9/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Homeodomain Proteins); 0 (Paired Box Transcription Factors); 0 (SOX9 Transcription Factor); 0 (homeobox protein PITX1)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170816
[St] Status:MEDLINE
[do] DOI:10.1242/dev.154864


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[PMID]:28771534
[Au] Autor:Suzuki H; Ahuja CS; Salewski RP; Li L; Satkunendrarajah K; Nagoshi N; Shibata S; Fehlings MG
[Ad] Endereço:Division of Genetics and Development, Krembil Research Institute, Toronto, Canada.
[Ti] Título:Neural stem cell mediated recovery is enhanced by Chondroitinase ABC pretreatment in chronic cervical spinal cord injury.
[So] Source:PLoS One;12(8):e0182339, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Traumatic spinal cord injuries (SCIs) affect millions of people worldwide; the majority of whom are in the chronic phase of their injury. Unfortunately, most current treatments target the acute/subacute injury phase as the microenvironment of chronically injured cord consists of a well-established glial scar with inhibitory chondroitin sulfate proteoglycans (CSPGs) which acts as a potent barrier to regeneration. It has been shown that CSPGs can be degraded in vivo by intrathecal Chondroitinase ABC (ChABC) to produce a more permissive environment for regeneration by endogenous cells or transplanted neural stem cells (NSCs) in the subacute phase of injury. Using a translationally-relevant clip-contusion model of cervical spinal cord injury in mice we sought to determine if ChABC pretreatment could modify the harsh chronic microenvironment to enhance subsequent regeneration by induced pluripotent stem cell-derived NSCs (iPS-NSC). Seven weeks after injury-during the chronic phase-we delivered ChABC by intrathecal osmotic pump for one week followed by intraparenchymal iPS-NSC transplant rostral and caudal to the injury epicenter. ChABC administration reduced chronic-injury scar and resulted in significantly improved iPSC-NSC survival with clear differentiation into all three neuroglial lineages. Neurons derived from transplanted cells also formed functional synapses with host circuits on patch clamp analysis. Furthermore, the combined treatment led to recovery in key functional muscle groups including forelimb grip strength and measures of forelimb/hindlimb locomotion assessed by Catwalk. This represents important proof-of-concept data that the chronically injured spinal cord can be 'unlocked' by ChABC pretreatment to produce a microenvironment conducive to regenerative iPS-NSC therapy.
[Mh] Termos MeSH primário: Condroitina ABC Liase/farmacologia
Regeneração Nervosa/efeitos dos fármacos
Traumatismos da Medula Espinal/terapia
[Mh] Termos MeSH secundário: Animais
Diferenciação Celular/efeitos dos fármacos
Células Cultivadas
Medula Cervical/lesões
Doença Crônica
Cicatriz/prevenção & controle
Potenciais Evocados/fisiologia
Membro Anterior/fisiologia
Células-Tronco Pluripotentes Induzidas/citologia
Locomoção/fisiologia
Camundongos
Camundongos Endogâmicos C57BL
Células-Tronco Neurais/citologia
Células-Tronco Neurais/efeitos dos fármacos
Células-Tronco Neurais/transplante
Neurônios/citologia
Neurônios/fisiologia
Recuperação de Função Fisiológica/efeitos dos fármacos
Medula Espinal/metabolismo
Medula Espinal/fisiologia
Traumatismos da Medula Espinal/patologia
Sinapses/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 4.2.2.20 (Chondroitin ABC Lyase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170804
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182339


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[PMID]:28735748
[Au] Autor:Miri A; Warriner CL; Seely JS; Elsayed GF; Cunningham JP; Churchland MM; Jessell TM
[Ad] Endereço:Department of Neuroscience, Columbia University, New York, NY 10032, USA; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA; Kavli Institute of Brain Science, Columbia University, New York, NY 10032, USA; Howard Hughes Medical Institute, Columbia Unive
[Ti] Título:Behaviorally Selective Engagement of Short-Latency Effector Pathways by Motor Cortex.
[So] Source:Neuron;95(3):683-696.e11, 2017 Aug 02.
[Is] ISSN:1097-4199
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Blocking motor cortical output with lesions or pharmacological inactivation has identified movements that require motor cortex. Yet, when and how motor cortex influences muscle activity during movement execution remains unresolved. We addressed this ambiguity using measurement and perturbation of motor cortical activity together with electromyography in mice during two forelimb movements that differ in their requirement for cortical involvement. Rapid optogenetic silencing and electrical stimulation indicated that short-latency pathways linking motor cortex with spinal motor neurons are selectively activated during one behavior. Analysis of motor cortical activity revealed a dramatic change between behaviors in the coordination of firing patterns across neurons that could account for this differential influence. Thus, our results suggest that changes in motor cortical output patterns enable a behaviorally selective engagement of short-latency effector pathways. The model of motor cortical influence implied by our findings helps reconcile previous observations on the function of motor cortex.
[Mh] Termos MeSH primário: Comportamento de Escolha/fisiologia
Córtex Motor/fisiologia
Neurônios Motores/fisiologia
Movimento/fisiologia
Vias Neurais/fisiologia
[Mh] Termos MeSH secundário: Animais
Eletromiografia/métodos
Membro Anterior/fisiologia
Masculino
Camundongos
Optogenética/métodos
Transmissão Sináptica/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170725
[St] Status:MEDLINE



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