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Pesquisa : A13.421 [Categoria DeCS]
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  1 / 7914 MEDLINE  
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[PMID]:28954371
[Au] Autor:Ajima MNO; Pandey PK; Kumar K; Poojary N
[Ad] Endereço:Department of Fisheries and Aquaculture Technology, Federal University of Technology, Owerri, Nigeria. Electronic address: malajimo@gmail.com.
[Ti] Título:Alteration in DNA structure, molecular responses and Na -K -ATPase activities in the gill of Nile tilapia, Oreochromis niloticus (Linnaeus, 1758) in response to sub-lethal verapamil.
[So] Source:Ecotoxicol Environ Saf;147:809-816, 2018 Jan.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The ecotoxicological consequences of residues from pharmaceutical drugs on aquatic biota have necessitated the development of sensitive and reliable techniques to assess the impact of these xenobiotics on aquatic organisms. This study investigated the alteration in DNA structure, molecular responses and the activities of Na -K -ATPase and antioxidant enzymes in the gill of Nile tilapia, Oreochromis niloticus, exposed to long-term effects at the concentrations (0.14, 0.28 and 0.57mgL ) of verapamil in static renewal system for 15, 30, 45 and 60 days. Evaluation of DNA structure, using single cell gel electrophoresis, revealed certain degree of DNA damages in the gill in a time and concentration-dependent relationship. Transcription of mRNA of superoxide dismutase (sod), catalase (cat) and heat shock protein (hsp70) genes in the gill of the fish showed the genes were up-regulated. Na -K -ATPase activity was inhibited in a concentration and time dependent manner. The indices of oxidative stress biomarkers (lipid peroxidation and carbonyl protein) as well as superoxide dismutase, glutathione peroxidase, glutathione-S-transferase were elevated in the treated fish in comparison to the control. Further, the level of reduced glutathione and catalase activity were inhibited at 0.28mgL after day 30. Long-term exposure to sub-lethal concentration of verapamil can cause DNA damages, molecular effects and oxidative stress in O. niloticus. The biomarkers analysed can be used as early warning signals in environmental biomonitoring and assessment of drug contamination in aquatic ecosystem.
[Mh] Termos MeSH primário: Ciclídeos/metabolismo
Dano ao DNA
Brânquias/efeitos dos fármacos
ATPase Trocadora de Sódio-Potássio/metabolismo
Verapamil/toxicidade
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Antioxidantes/metabolismo
Biomarcadores/metabolismo
Ciclídeos/genética
Relação Dose-Resposta a Droga
Monitoramento Ambiental/métodos
Brânquias/metabolismo
Estresse Oxidativo/efeitos dos fármacos
Verapamil/metabolismo
Poluentes Químicos da Água/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Biomarkers); 0 (Water Pollutants, Chemical); CJ0O37KU29 (Verapamil); EC 3.6.3.9 (Sodium-Potassium-Exchanging ATPase)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170929
[St] Status:MEDLINE


  2 / 7914 MEDLINE  
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[PMID]:29257630
[Au] Autor:Saylor Ö
[Ti] Título:Toxic effects of permethrin on Pseudorasbora parva.
[So] Source:J Environ Biol;37(6):1247-53, 2016 Nov.
[Is] ISSN:0254-8704
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:The present study deals with acute toxicity and hematological, histopathological and genotoxical effects of permethrin, a synthetic pyrethroid, on Pseudorasbora parva. Acute toxicity of 96-hrs LC50 value was found to be 88.25 (84.60-92.63) µgl-1. Sublethal dose was taken as 8.82 µgl-1 (the 1/10 of the LC50 value) in bio-experiments. Micronucleus changes in blood erythrocyte and hemotocyte level in P. parva exposed to sub-lethal concentration were investigated. Difference between nucleolus abnormalities in the experimental and control group was found to be statistically significant (t-test). Micronucleus frequency was found to be 8.26. The hematocrit level in control and experimental groups were found to be 24.43% and 14.673%. No pathological symptoms were observed in the muscle of P. parva exposed to sublethal dose for 96 hrs. Pathological symptoms observed after 96 hours from permethrin administration in other organs were: Fusion, Telangiectasis, epithelial lifting and hyperemia in gills; hydropic degeneration, lipid degeneration and passive hyperemia in liver and enlargement of cavum glomeruli and Bowman space in kidneys and hemorrhage, edema and hyperemia in brain, respectively.
[Mh] Termos MeSH primário: Cyprinidae
Inseticidas/toxicidade
Permetrina/toxicidade
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Encéfalo/efeitos dos fármacos
Encéfalo/patologia
Brânquias/efeitos dos fármacos
Brânquias/patologia
Espécies Introduzidas
Rim/efeitos dos fármacos
Rim/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insecticides); 0 (Water Pollutants, Chemical); 509F88P9SZ (Permethrin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE


  3 / 7914 MEDLINE  
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[PMID]:29281662
[Au] Autor:Kimmel CB; Small CM; Knope ML
[Ad] Endereço:Institute of Neuroscience, University of Oregon, Eugene, Oregon, United States of America.
[Ti] Título:A rich diversity of opercle bone shape among teleost fishes.
[So] Source:PLoS One;12(12):e0188888, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The opercle is a prominent craniofacial bone supporting the gill cover in all bony fish and has been the subject of morphological, developmental, and genetic investigation. We surveyed the shapes of this bone among 110 families spanning the teleost tree and examined its pattern of occupancy in a principal component-based morphospace. Contrasting with expectations from the literature that suggest the local morphospace would be only sparsely occupied, we find primarily dense, broad filling of the morphological landscape, indicating rich diversity. Phylomorphospace plots suggest that dynamic evolution underlies the observed spatial patterning. Evolutionary transits through the morphospaces are sometimes long, and occur in a variety of directions. The trajectories seem to represent both evolutionary divergences and convergences, the latter supported by convevol analysis. We suggest that that this pattern of occupancy reflects the various adaptations of different groups of fishes, seemingly paralleling their diverse marine and freshwater ecologies and life histories. Opercle shape evolution within the acanthomorphs, spiny ray-finned fishes, appears to have been especially dynamic.
[Mh] Termos MeSH primário: Osso e Ossos/anatomia & histologia
Peixes/anatomia & histologia
Brânquias/anatomia & histologia
[Mh] Termos MeSH secundário: Animais
Evolução Biológica
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, P.H.S.
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171228
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188888


  4 / 7914 MEDLINE  
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[PMID]:27775698
[Au] Autor:König S; Gros O; Heiden SE; Hinzke T; Thürmer A; Poehlein A; Meyer S; Vatin M; Mbéguié-A-Mbéguié D; Tocny J; Ponnudurai R; Daniel R; Becher D; Schweder T; Markert S
[Ad] Endereço:Department of Pharmaceutical Biotechnology, E.M.A. University of Greifswald, Institute of Pharmacy, Greifswald, Germany.
[Ti] Título:Nitrogen fixation in a chemoautotrophic lucinid symbiosis.
[So] Source:Nat Microbiol;2:16193, 2016 Oct 24.
[Is] ISSN:2058-5276
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The shallow water bivalve Codakia orbicularis lives in symbiotic association with a sulfur-oxidizing bacterium in its gills. The endosymbiont fixes CO and thus generates organic carbon compounds, which support the host's growth. To investigate the uncultured symbiont's metabolism and symbiont-host interactions in detail we conducted a proteogenomic analysis of purified bacteria. Unexpectedly, our results reveal a hitherto completely unrecognized feature of the C. orbicularis symbiont's physiology: the symbiont's genome encodes all proteins necessary for biological nitrogen fixation (diazotrophy). Expression of the respective genes under standard ambient conditions was confirmed by proteomics. Nitrogenase activity in the symbiont was also verified by enzyme activity assays. Phylogenetic analysis of the bacterial nitrogenase reductase NifH revealed the symbiont's close relationship to free-living nitrogen-fixing Proteobacteria from the seagrass sediment. The C. orbicularis symbiont, here tentatively named 'Candidatus Thiodiazotropha endolucinida', may thus not only sustain the bivalve's carbon demands. C. orbicularis may also benefit from a steady supply of fixed nitrogen from its symbiont-a scenario that is unprecedented in comparable chemoautotrophic symbioses.
[Mh] Termos MeSH primário: Bivalves/microbiologia
Crescimento Quimioautotrófico
Gammaproteobacteria/fisiologia
Fixação de Nitrogênio
Simbiose
[Mh] Termos MeSH secundário: Animais
Gammaproteobacteria/química
Gammaproteobacteria/genética
Gammaproteobacteria/metabolismo
Brânquias/microbiologia
Redes e Vias Metabólicas/genética
Nitrogenase/metabolismo
Oxirredutases/genética
Filogenia
Proteoma/análise
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Proteome); EC 1.- (Oxidoreductases); EC 1.18.6.1 (Nitrogenase); EC 1.18.6.1 (nitrogenase reductase)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180126
[Lr] Data última revisão:
180126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE
[do] DOI:10.1038/nmicrobiol.2016.193


  5 / 7914 MEDLINE  
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[PMID]:28743066
[Au] Autor:Kim JH; Park HJ; Hwang IK; Han JM; Kim DH; Oh CW; Lee JS; Kang JC
[Ad] Endereço:West Sea Fisheries Research Institute, National Institute of Fisheries Science, Incheon, Republic of Korea.
[Ti] Título:Toxic effects of juvenile sablefish, Anoplopoma fimbria by ammonia exposure at different water temperature.
[So] Source:Environ Toxicol Pharmacol;54:169-176, 2017 Sep.
[Is] ISSN:1872-7077
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Juvenile sablefish, Anoplopoma fimbria (mean length 17.1±2.4cm, and mean weight 75.6±5.7g) were used to evaluate toxic effects on antioxidant systems, immune responses, and stress indicators by ammonia exposure (0, 0.25, 0.75, and 1.25mg/L) at different water temperature (12 and 17°C) in 1 and 2 months. In antioxidant responses, superoxide dismutase (SOD) and catalase (CAT) were significantly increased by ammonia exposure, whereas glutathione (GSH) was decreased. In immune responses, lysozyme and phagocytosis activity were significantly increased by ammonia exposure. In stress indicators, plasma glucose, heat shock protein 70 (HSP 70), and cortisol were significantly increased. At high water temperature (17°C), alterations by ammonia exposure were more distinctly. The results of this study indicated that ammonia exposure can induce toxic effects in the sablefish, and high water temperature can affect the ammonia exposure toxicity.
[Mh] Termos MeSH primário: Amônia/toxicidade
Perciformes
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Catalase/metabolismo
Proteínas de Peixes/metabolismo
Brânquias/efeitos dos fármacos
Brânquias/metabolismo
Glutationa/metabolismo
Proteínas de Choque Térmico HSP70/metabolismo
Hidrocortisona/sangue
Rim/efeitos dos fármacos
Rim/metabolismo
Fígado/efeitos dos fármacos
Fígado/metabolismo
Micrococcus
Muramidase/sangue
Muramidase/metabolismo
Perciformes/sangue
Perciformes/imunologia
Perciformes/metabolismo
Fagocitose
Superóxido Dismutase/metabolismo
Temperatura Ambiente
Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fish Proteins); 0 (HSP70 Heat-Shock Proteins); 0 (Water Pollutants, Chemical); 059QF0KO0R (Water); 7664-41-7 (Ammonia); EC 1.11.1.6 (Catalase); EC 1.15.1.1 (Superoxide Dismutase); EC 3.2.1.17 (Muramidase); GAN16C9B8O (Glutathione); WI4X0X7BPJ (Hydrocortisone)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171130
[Lr] Data última revisão:
171130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE


  6 / 7914 MEDLINE  
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[PMID]:28470783
[Au] Autor:Kryukova NV
[Ad] Endereço:Laboratory of morphological adaptations of vertebrates, Severtsov Institute of Ecology and Evolution, RAS, Moscow, 119071, Russia.
[Ti] Título:Functional analysis of the musculo-skeletal system of the gill apparatus in Heptranchias perlo (Chondrichthyes: Hexanchidae).
[So] Source:J Morphol;278(8):1075-1090, 2017 Aug.
[Is] ISSN:1097-4687
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Musculo-skeletal morphology is an indispensable source for understanding functional adaptations. Analysis of morphology of the branchial apparatus of Hexanchiform sharks can provide insight into aspects of their respiration that are difficult to observe directly. In this study, I compare the structure of the musculo-skeletal system of the gill apparatus of Heptranchias perlo and Squalus acanthias in respect to their adaptation for one of two respiratory mechanisms known in sharks, namely, the active two-pump (oropharyngeal and parabranchial) ventilation and the ram-jet ventilation. In both species, the oropharyngeal pump possesses two sets of muscles, one for compression and the other for expansion. The parabranchial pump only has constrictors. Expansion of this pump occurs only due to passive elastic recoil of the extrabranchial cartilages. In Squalus acanthias the parabranchial chambers are large and equipped by powerful superficial constrictors. These muscles and the outer walls of the parabranchial chambers are much reduced in Heptranchias perlo, and thus it likely cannot use this pump. However, this reduction allows for vertical elongation of outer gill slits which, along with greater number of gill pouches, likely decreases branchial resistance and, at the same time, increases the gill surface area, and can be regarded as an adaptation for ram ventilation at lower speeds.
[Mh] Termos MeSH primário: Brânquias/anatomia & histologia
Sistema Musculoesquelético/anatomia & histologia
Tubarões/anatomia & histologia
Tubarões/fisiologia
[Mh] Termos MeSH secundário: Animais
Osso e Ossos/anatomia & histologia
Cabeça/anatomia & histologia
Músculos/anatomia & histologia
Squalus acanthias/anatomia & histologia
Squalus acanthias/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1002/jmor.20695


  7 / 7914 MEDLINE  
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[PMID]:29073147
[Au] Autor:Chng YR; Ong JLY; Ching B; Chen XL; Hiong KC; Wong WP; Chew SF; Lam SH; Ip YK
[Ad] Endereço:Department of Biological Sciences, National University of Singapore, Singapore.
[Ti] Título:Molecular characterization of three Rhesus glycoproteins from the gills of the African lungfish, Protopterus annectens, and effects of aestivation on their mRNA expression levels and protein abundance.
[So] Source:PLoS One;12(10):e0185814, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:African lungfishes are ammonotelic in water. They can aestivate for long periods on land during drought. During aestivation, the gills are covered with dried mucus and ammonia excretion ceases. In fishes, ammonia excretion through the gills involves Rhesus glycoproteins (RhGP/Rhgp). This study aimed to obtain the complete cDNA coding sequences of rhgp from the gills of Protopterus annectens, and to determine their branchial mRNA and protein expression levels during the induction, maintenance and arousal phases of aestivation. Three isoforms of rhgp (rhag, rhbg and rhcg) were obtained in the gills of P. annectens. Their complete cDNA coding sequences ranged between 1311 and 1398 bp, coding for 436 to 465 amino acids with estimated molecular masses between 46.8 and 50.9 kDa. Dendrogramic analyses indicated that Rhag was grouped closer to fishes, while Rhbg and Rhcg were grouped closer to tetrapods. During the induction phase, the protein abundance of Rhag, but not its transcript level, was down-regulated in the gills, suggesting that there could be a decrease in the release of ammonia from the erythrocytes to the plasma. Furthermore, the branchial transcript levels of rhbg and rhcg decreased significantly, in preparation for the subsequent shutdown of gill functions. During the maintenance phase, the branchial expression levels of rhag/Rhag, rhbg/Rhbg and rhcg/Rhcg decreased significantly, indicating that their transcription and translation were down-regulated. This could be part of an overall mechanism to shut down branchial functions and save metabolic energy used for transcription and translation. It could also be regarded as an adaptive response to stop ammonia excretion. During the arousal phase, it is essential for the lungfish to regain the ability to excrete ammonia. Indeed, the protein abundance of Rhag, Rhbg and Rhcg recovered to the corresponding control levels after 1 day or 3 days of recovery from 6 months of aestivation.
[Mh] Termos MeSH primário: Peixes/genética
Brânquias/metabolismo
Glicoproteínas/genética
RNA Mensageiro/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Regulação para Baixo
Homologia de Sequência de Aminoácidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glycoproteins); 0 (RNA, Messenger)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171027
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185814


  8 / 7914 MEDLINE  
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[PMID]:28972451
[Au] Autor:Giacomin M; Schulte PM; Wood CM
[Ti] Título:Differential Effects of Temperature on Oxygen Consumption and Branchial Fluxes of Urea, Ammonia, and Water in the Dogfish Shark (Squalus acanthias suckleyi).
[So] Source:Physiol Biochem Zool;90(6):627-637, 2017 Nov/Dec.
[Is] ISSN:1537-5293
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Environmental temperature can greatly influence the homeostasis of ectotherms through its effects on biochemical reactions and whole-animal physiology. Elasmobranchs tend to be N limited and are osmoconformers, retaining ammonia and urea-N at the gills and using the latter as a key osmolyte to maintain high blood osmolality. However, the effects of temperature on these key processes remain largely unknown. We evaluated the effects of acute exposure to different temperatures (7°, 12°, 15°, 18°, 22°C) on oxygen consumption, ammonia, urea-N, and diffusive water fluxes at the gills of Squalus acanthias suckleyi. We hypothesized that as metabolic demand for oxygen increased with temperature, the fluxes of ammonia, urea-N, and H O at the gills would increase in parallel with those of oxygen. Oxygen consumption (overall [Formula: see text] from 7.5° to 22°C) and water fluxes (overall [Formula: see text]) responded to increases in temperature in a similar, almost linear, manner. Ammonia-N efflux rates varied the most, increasing almost 15-fold from 7.5° to 22°C ([Formula: see text]). Urea-N efflux was tightly conserved over the 7.5°-15°C range ([Formula: see text]) but increased greatly at higher temperatures, yielding an overall [Formula: see text]. These differences likely reflect differences in the transport pathways for the four moieties. They also suggest the failure of urea-N- and ammonia-N-conserving mechanisms at the gill above 15°C. Hyperoxia did not alleviate the effects of high temperature. Indeed, urea-N and ammonia-N effluxes were dramatically increased when animals were exposed to high temperatures in the presence of hyperoxia, suggesting that high partial pressure of oxygen may have caused oxidative damage to gill epithelial membranes.
[Mh] Termos MeSH primário: Amônia/metabolismo
Consumo de Oxigênio/fisiologia
Tubarões/fisiologia
Temperatura Ambiente
Ureia/metabolismo
Água/metabolismo
[Mh] Termos MeSH secundário: Animais
Brânquias/fisiologia
Nitrogênio/metabolismo
Permeabilidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
059QF0KO0R (Water); 7664-41-7 (Ammonia); 8W8T17847W (Urea); N762921K75 (Nitrogen)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171004
[St] Status:MEDLINE
[do] DOI:10.1086/694296


  9 / 7914 MEDLINE  
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[PMID]:28918749
[Au] Autor:Wu M; Chen N; Huang CX; He Y; Zhao YZ; Chen XH; Chen XL; Wang HL
[Ad] Endereço:Ministry of Education, Huazhong Agricultural University, College of Fishery, Key Lab of Freshwater Animal Breeding, Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction, Wuhan, 430070, PR China. hbauwhl@hotmail.com.
[Ti] Título:Effect of Low Temperature on Globin Expression, Respiratory Metabolic Enzyme Activities, and Gill Structure of Litopenaeus vannamei.
[So] Source:Biochemistry (Mosc);82(7):844-851, 2017 Jul.
[Is] ISSN:1608-3040
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Low temperature frequently influences growth, development, and even survival of aquatic animals. In the present study, physiological and molecular responses to low temperature in Litopenaeus vannamei were investigated. The cDNA sequences of two oxygen-carrying proteins, cytoglobin (Cygb) and neuroglobin (Ngb), were isolated. Protein structure analysis revealed that both proteins share a globin superfamily domain. Real-time PCR analysis indicated that Cygb and Ngb mRNA levels gradually increased during decrease in temperatures from 25 to 15°C and then decreased at 10°C in muscle, brain, stomach, and heart, except for a continuing increase in gills, whereas they showed a different expression trend in the hepatopancreas. Hemocyanin concentration gradually reduced as the temperature decreased. Moreover, the activities of respiratory metabolic enzymes including lactate dehydrogenase (LDH) and succinate dehydrogenase (SDH) were measured, and it was found that LDH activity gradually increased while SDH activity decreased after low-temperature treatment. Finally, damage to gill structure at low temperature was also observed, and this intensified with further decrease in temperature. Taken together, these results show that low temperature has an adverse influence in L. vannamei, which contributes to systematic understanding of the adaptation mechanisms of shrimp at low temperature.
[Mh] Termos MeSH primário: Temperatura Baixa
Regulação da Expressão Gênica
Globinas/genética
Proteínas do Tecido Nervoso/genética
Penaeidae/anatomia & histologia
Penaeidae/fisiologia
[Mh] Termos MeSH secundário: Animais
Bases de Dados Factuais
Brânquias/anatomia & histologia
Brânquias/metabolismo
Globinas/química
Globinas/metabolismo
Hemocianinas/análise
Hepatopâncreas/metabolismo
L-Lactato Desidrogenase/genética
L-Lactato Desidrogenase/metabolismo
Proteínas do Tecido Nervoso/química
Proteínas do Tecido Nervoso/metabolismo
Penaeidae/enzimologia
Penaeidae/genética
RNA Mensageiro/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
Succinato Desidrogenase/genética
Succinato Desidrogenase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nerve Tissue Proteins); 0 (RNA, Messenger); 0 (cytoglobin); 0 (neuroglobin); 9004-22-2 (Globins); 9013-72-3 (Hemocyanins); EC 1.1.1.27 (L-Lactate Dehydrogenase); EC 1.3.99.1 (Succinate Dehydrogenase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170919
[St] Status:MEDLINE
[do] DOI:10.1134/S0006297917070100


  10 / 7914 MEDLINE  
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[PMID]:28865209
[Au] Autor:Strzyzewska-Worotynska E; Szarek J; Babinska I; Gulda D
[Ad] Endereço:.
[Ti] Título:Impact of production technology on morphological lesions in the gills of commercial rainbow trout (Oncorhynchus mykiss, Walbaum 1792).
[So] Source:Pol J Vet Sci;20(2):233-240, 2017 Mar 01.
[Is] ISSN:1505-1773
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The most popular rainbow trout (Oncorhynchus mykiss, Walbaum 1792) production technologies include both an extensive method with the flow through system (FTS) and an intensive method with the recirculating aquaculture system (RAS). Their impact on the fish was evaluated with a morphological assessment of the gills, as these are organs susceptible to environmental changes. Trout of 350 - 500 g body mass were caught for trial in spring and autumn, with 36 fish originating from 3 fish farms with the FTS system and an equal number from 3 RAS fish farms (n = 6). The fish were macroscopically examined and the gills were investigated microscopically (haematoxylin and eosin staining). Hypertrophy and hyperplasia were most commonly detected, amounting to 89% of all structural abnormalities. These lesions were slightly more common in the FTS, especially in autumn, whereas the changes to the blood vessels in the gills were observed more frequently in the rainbow trout from the RAS system than in the fish obtained from the FTS technology (the difference was statistically significant). The morphological lesions in the mucous cells of the gills were detected at a comparable severity regardless of the technology or production season. The predominantly low values of the histopathologic indices, which described the microscopic lesions in the gills of fish from the FTS and RAS systems, showed that the examined organ was most often free of lesions or demonstrated only minor morphological damage regardless of the production technology.
[Mh] Termos MeSH primário: Criação de Animais Domésticos/métodos
Aquicultura/métodos
Doenças dos Peixes/etiologia
Brânquias/patologia
Oncorhynchus mykiss
[Mh] Termos MeSH secundário: Animais
Abrigo para Animais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170903
[St] Status:MEDLINE



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