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[PMID]: | 28260650 |
[Au] Autor: | Xing J; Ma J; Tang X; Sheng X; Zhan W |
[Ad] Endereço: | Laboratory of Pathology and Immunology of Aquatic Animals,KLMME, Ocean University of China, Qingdao 266003, PR China. |
[Ti] Título: | Characterizations of CD4-1, CD4-2 and CD8ß T cell subpopulations in peripheral blood leucocytes, spleen and head kidney of Japanese flounder (Paralichthys olivaceus). |
[So] Source: | Mol Immunol;85:155-165, 2017 May. | [Is] ISSN: | 1872-9142 |
[Cp] País de publicação: | England |
[La] Idioma: | eng |
[Ab] Resumo: | In the previous study, antibodies against CD3 molecule have been produced and were used in labeling T cells in Japanese flounder (Paralichthys olivaceus). In this paper, CD4 and CD8 lymphocytes subpopulations in peripheral blood leucocytes (PBL), spleen and head kidney of flounder were investigated. The flounder CD4-1, CD4-2 and CD8ß recombinant proteins and their antibodies (Abs) were produced, then the cross-reactivity of the Abs to CD4-1, CD4-2 and CD8ß was detected by Western blotting, respectively, and the reactions of Abs to PBL were analyzed by immunofluorescence staining (IFS) and flow cytometry (FCM). CD4-1 /CD3 , CD4-2 /CD3 , and CD8ß /CD3 lymphocytes in PBL, spleen and head kidney were observed by double IFS, then their proportions were analyzed using two-color FCM, respectively. Further, CD4-1/CD8ß, CD4-2/CD8ß, or CD4-1/CD4-2 lymphocytes were analyzed using double-IFS and two-color FCM. Finally, CD4-1 , CD4-2 , and CD8ß lymphocytes in spleen and head kidney were observed by immunohistochemistry. The results showed that the Abs were specific for CD4-1, CD4-2 and CD8ß molecules, respectively. The proportions of CD4-1 /CD3 , CD4-2 /CD3 , and CD8ß /CD3 lymphocytes were 6.7±2.0%, 8.6±2.8%, 2.1±1.3% in PBL; 13.6±3.6%, 15.6±5.2%, 2.8±1.4% in spleen; 20.0±4.6%, 20.5±4.6%, 3.2±1.5% in head kidney, respectively. Most CD4 and CD8 cell subpopulations belonged to CD3 cells; there were no cross-reactivity between CD4 and CD8 cells. CD4-1 /CD4-2 , CD4-1 /CD4-2 , and CD4-1 /CD4-2 cells presented different proportions in PBL, spleen and head kidney, among them, CD4-1 /CD4-2 cell is the majority of CD4T cell subpopulation. |
[Mh] Termos MeSH primário: |
Linguado/imunologia Rim Cefálico/imunologia Leucócitos/imunologia Baço/imunologia Subpopulações de Linfócitos T/imunologia
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[Mh] Termos MeSH secundário: |
Animais Western Blotting Linfócitos T CD4-Positivos/citologia Linfócitos T CD4-Positivos/imunologia Linfócitos T CD8-Positivos/citologia Linfócitos T CD8-Positivos/imunologia Citometria de Fluxo Imunofluorescência Rim Cefálico/citologia Leucócitos/citologia Baço/citologia
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Em] Mês de entrada: | 1709 |
[Cu] Atualização por classe: | 170915 |
[Lr] Data última revisão:
| 170915 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 170307 |
[St] Status: | MEDLINE |
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