Base de dados : MEDLINE Pesquisa : A13.449 [Categoria DeCS] Referências encontradas : 326 [refinar] Mostrando: 1 .. 10   no formato [Detalhado]

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[PMID]: 28601517 [Au] Autor: Zeng L; Zhang JS; Zheng JL; Wu CW [Ad] Endereço: National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhoushan 316022, PR China. Electronic address: zenglin615@126.com. [Ti] Título: Pre-acclimation to low copper mitigated immunotoxic effects in spleen and head-kidney of large yellow croaker (Pseudosciaena crocea) when exposed subsequently to high copper. [So] Source: Ecotoxicol Environ Saf;144:54-61, 2017 Oct. [Is] ISSN: 1090-2414 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: The hypothesis tested in this study was that Cu pre-acclimation would mitigate high Cu induced immunotoxic effects in large yellow croaker Pseudosciaena crocea. To the end, fish were pre-acclimation to 0 and 84µg CuL for 48h and then exposed to 0 and 420µg CuL for another 48h. Survival rate, Cu content, ROS, NO, activities and mRNA levels of inflammatory genes (iNOS and COX-2), and gene expressions of transcription factor NF-κB and its inhibitor IκBα were determined in spleen and head-kidney of large yellow croaker. Cu pre-acclimation significantly reduced mortality of fish exposed to 420µg CuL . Cu pre-acclimation triggered the up-regulation of both enzyme activities and express levels of iNOS and COX-2 in spleen under 420µg CuL exposure, resulting in remarkable reduction of Cu content and ROS in this tissue. Contrast to spleen, iNOS activity remained unchanged but the mRNA level of iNOS increased, and the mRNA level of COX-2 remained constant though COX-2 activity enhanced in head-kidney, suggesting iNOS and COX-2 may be modulated by Cu at a post-transcriptional level. In this process, NF-κB/IκBα signaling molecules may play a vital role in the transcriptional activation of inflammatory genes in both spleen and head-kidney. In conclusion, low Cu pre-acclimation alleviated high Cu induced immunotoxicity in spleen and head-kidney of large yellow croaker by enhancing the activities and mRNA levels of inflammatory genes. [Mh] Termos MeSH primário: Aclimatação/efeitos dos fármacos Cobre/toxicidade Rim Cefálico/efeitos dos fármacos Perciformes/imunologia Baço/efeitos dos fármacos Poluentes Químicos da Água/toxicidade [Mh] Termos MeSH secundário: Aclimatação/genética Animais Cobre/análise Relação Dose-Resposta a Droga Proteínas de Peixes/genética Expressão Gênica/efeitos dos fármacos Rim Cefálico/imunologia Perciformes/genética Perciformes/metabolismo RNA Mensageiro/genética Baço/imunologia Regulação para Cima Poluentes Químicos da Água/análise [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Fish Proteins); 0 (RNA, Messenger); 0 (Water Pollutants, Chemical); 789U1901C5 (Copper) [Em] Mês de entrada: 1711 [Cu] Atualização por classe: 171109 [Lr] Data última revisão: 171109 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170612 [St] Status: MEDLINE

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[PMID]: 28407501 [Au] Autor: Curtis LR; Bravo CF; Bayne CJ; Tilton F; Arkoosh MR; Lambertini E; Loge FJ; Collier TK; Meador JP; Tilton SC [Ad] Endereço: Department of Environmental and Molecular Toxicology, Oregon State University, ALS 1007, Corvallis, OR 97331, USA. [Ti] Título: Transcriptional changes in innate immunity genes in head kidneys from Aeromonas salmonicida-challenged rainbow trout fed a mixture of polycyclic aromatic hydrocarbons. [So] Source: Ecotoxicol Environ Saf;142:157-163, 2017 Aug. [Is] ISSN: 1090-2414 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: We previously observed that exposure to a complex mixture of high molecular weight polycyclic aromatic hydrocarbons (PAHs) increased sensitivity of rainbow trout (Oncorhynchus mykiss) to subsequent challenge with Aeromonas salmonicida, the causative agent of furunculosis. In this study, we evaluate potential mechanisms associated with disease susceptibility from combined environmental factors of dietary PAH exposure and pathogen challenge. Rainbow trout were fed a mixture of ten high molecular weight PAHs at an environmentally relevant concentration (7.82µg PAH mixture/g fish/day) or control diet for 50 days. After 50 days of PAH exposure, fish were challenged with either Aeromonas salmonicida at a lethal concentration 30 (LC ) or growth media without the pathogen (mock challenge). Head kidneys were collected 2, 4, 10 and 20 days after challenge and gene expression (q<0.05) was evaluated among treatments. In animals fed the PAH contaminated diet, we observed down-regulation of expression for innate immune system genes in pathways (p<0.05) for the terminal steps of the complement cascade (complement component C6) and other bacteriolytic processes (lysozyme type II) potentially underlying increased disease susceptibility after pathogen challenge. Increased expression of genes associated with hemorrhage/tissue remodeling/inflammation pathways (p<0.05) was likely related to more severe head kidney damage due to infection in PAH-fed compared to control-fed fish. This study is the first to evaluate transcriptional signatures associated with the impact of chronic exposure to an environmentally relevant mixture of PAHs in disease susceptibility and immunity. [Mh] Termos MeSH primário: Aeromonas salmonicida/patogenicidade Rim Cefálico/imunologia Imunidade Inata/efeitos dos fármacos Oncorhynchus mykiss/microbiologia Hidrocarbonetos Aromáticos Policíclicos/toxicidade Transcrição Genética/efeitos dos fármacos [Mh] Termos MeSH secundário: Animais Regulação para Baixo Imunidade Inata/genética Muramidase/metabolismo Oncorhynchus mykiss/imunologia Oncorhynchus mykiss/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Polycyclic Aromatic Hydrocarbons); EC 3.2.1.17 (Muramidase) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170922 [Lr] Data última revisão: 170922 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170414 [St] Status: MEDLINE

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[PMID]: 28359945 [Au] Autor: Torrecillas S; Román L; Rivero-Ramírez F; Caballero MJ; Pascual C; Robaina L; Izquierdo MS; Acosta F; Montero D [Ad] Endereço: Grupo de Investigación en Acuicultura (GIA), IU-ECOAQUA, Universidad de Las Palmas de Gran Canaria, Crta. Taliarte s/n, 35214 Telde, Las Palmas, Canary Islands, Spain. Electronic address: silvia.torrecillas@giaqua.org. [Ti] Título: Supplementation of arachidonic acid rich oil in European sea bass juveniles (Dicentrarchus labrax) diets: Effects on leucocytes and plasma fatty acid profiles, selected immune parameters and circulating prostaglandins levels. [So] Source: Fish Shellfish Immunol;64:437-445, 2017 May. [Is] ISSN: 1095-9947 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: The main objective of this study was to assess the effects of graded levels of dietary arachidonic acid (ARA), supplemented from alternative sources, on fatty acid composition of plasma and head kidney leucocytes of European sea bass (Dicentrarchus labrax). For that purpose, sea bass juveniles were fed four diets containing graded levels of ARA as follows: 0.5% (ARA0.5), 1% (ARA1), 2% (ARA2) and 4% (ARA4) during 60 days. At the end of the feeding trial fatty acid profiles of plasma and head kidney leucocytes were analyzed. Besides, plasma prostaglandins levels, head kidney leucocytes respiratory burst activity; peroxidase activity and phagocytic index were assayed. Reducing dietary ARA levels below 1% markedly reduced European sea bass growth performance. However, fish fed diet ARA0.5 tried to compensate this dietary ARA deficiency by a selective deposition of ARA on plasma and head kidney leucocytes, reaching similar levels to those fish fed diet ARA1 after 60 days of feeding. Nevertheless, head kidney phagocytic capacity was reduced as dietary ARA content in relation not only to variations on membrane composition but also to changes on fish basal prostaglandins levels. Results obtained demonstrated the importance to supply the necessary quantity n-6 LC-PUFA, and not only n-3 LC-PUFA levels, in European sea bass diets, in relation to not only growth performance but also immune system function. [Mh] Termos MeSH primário: Ácido Araquidônico/metabolismo Bass/imunologia Suplementos Nutricionais Ácidos Graxos/sangue Rim Cefálico/imunologia Leucócitos/imunologia Prostaglandinas/sangue [Mh] Termos MeSH secundário: Ração Animal/análise Animais Ácido Araquidônico/administração & dosagem Bass/sangue Bass/metabolismo Dieta/veterinária Suplementos Nutricionais/análise Relação Dose-Resposta a Droga Distribuição Aleatória [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Fatty Acids); 0 (Prostaglandins); 27YG812J1I (Arachidonic Acid) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170628 [Lr] Data última revisão: 170628 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170401 [St] Status: MEDLINE

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[PMID]: 28347744 [Au] Autor: Seemann F; Peterson DR; Chiang MWL; Au DWT [Ad] Endereço: State Key Laboratory in Marine Pollution, Department of Biology and Chemistry, City University of Hong Kong, Hong Kong Special Administrative Region. [Ti] Título: The development of cellular immune defence in marine medaka Oryzias melastigma. [So] Source: Comp Biochem Physiol C Toxicol Pharmacol;199:81-89, 2017 Sep. [Is] ISSN: 1532-0456 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Environmentally induced alterations of the immune system during sensitive developmental stages may manifest as abnormalities in immune organ configuration and/or immune cell differentiation. These not only render the early life stages more vulnerable to pathogens, but may also affect the adult immune competence. Knowledge of these sensitive periods in fish would provide an important prognostic/diagnostic tool for aquatic risk assessment of immunotoxicants. The marine medaka Oryzias melastigma is an emerging seawater fish model for immunotoxicology. Here, the presence and onset of four potentially sensitive periods during the development of innate and adaptive cellular immune defence were revealed in O. melastigma: 1.) initiation of phagocyte differentiation, 2.) migration and expansion of lymphoid progenitor cells, 3.) colonization of immune organs through lymphocyte progenitors and 4.) establishment of immune competence in the thymus. By using an established bacterial resistance assay for O. melastigma, larval immune competence (from newly hatched 1dph to 14dph) was found concomitantly increased with advanced thymus development and the presence of mature T-lymphocytes. A comparison between the marine O. melastigma and the freshwater counterpart Oryzias latipes disclosed a disparity in the T-lymphocyte maturation pattern, resulting in differences in the length of T-lymphocyte maturation. The results shed light on a potential difference between seawater and freshwater medaka in their sensitivity to environmental immunotoxicants. Further, medaka immune system development was compared and contrasted to economically important fish. The present study has provided a strong scientific basis for advanced investigation of critical windows for immune system development in fish. [Mh] Termos MeSH primário: Embrião não Mamífero/imunologia Imunidade Celular Imunidade Inata Imunocompetência Larva/imunologia Morfogênese Oryzias/imunologia [Mh] Termos MeSH secundário: Animais Aquicultura Carga Bacteriana Diferenciação Celular Edwardsiella tarda/crescimento & desenvolvimento Edwardsiella tarda/imunologia Edwardsiella tarda/isolamento & purificação Embrião não Mamífero/citologia Embrião não Mamífero/microbiologia Desenvolvimento Embrionário Regulação da Expressão Gênica no Desenvolvimento Rim Cefálico/citologia Rim Cefálico/crescimento & desenvolvimento Rim Cefálico/imunologia Rim Cefálico/microbiologia Hibridização In Situ/veterinária Larva/citologia Larva/crescimento & desenvolvimento Larva/microbiologia Células Progenitoras Linfoides/citologia Células Progenitoras Linfoides/imunologia Células Progenitoras Linfoides/microbiologia Oryzias/embriologia Oryzias/crescimento & desenvolvimento Oryzias/microbiologia Fagócitos/citologia Fagócitos/imunologia Fagócitos/microbiologia Especificidade da Espécie Baço/citologia Baço/crescimento & desenvolvimento Baço/imunologia Baço/microbiologia Análise de Sobrevida Linfócitos T/citologia Linfócitos T/imunologia Linfócitos T/microbiologia Timo/citologia Timo/crescimento & desenvolvimento Timo/imunologia Timo/microbiologia [Pt] Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 170809 [Lr] Data última revisão: 170809 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170329 [St] Status: MEDLINE

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[PMID]: 28286256 [Au] Autor: Ji L; Sun G; Li J; Wang Y; Du Y; Li X; Liu Y [Ad] Endereço: Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China; University of Chinese Academy of Sciences, Beijing, 100039, China. [Ti] Título: Effect of dietary ß-glucan on growth, survival and regulation of immune processes in rainbow trout (Oncorhynchus mykiss) infected by Aeromonas salmonicida. [So] Source: Fish Shellfish Immunol;64:56-67, 2017 May. [Is] ISSN: 1095-9947 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: The present study evaluated the effects of dietary ß-glucan (0, 0.05%, 0.1%, and 0.2%) on growth performance after 42 days of feeding. Thereafter, rainbow trout (Oncorhynchus mykiss) were infected with Aeromonas salmonicida, and survival rates as well as the regulating processes of stress- and immune-related factors were analyzed. In general, higher dietary ß-glucan levels obviously improved specific growth rate (SGR), weight gain (WG) and feed efficiency (FE) (P ≤ 0.05). Survival rates in ß-glucan groups increased significantly compared with the control group after A. salmonicida infection (P ≤ 0.05). Serum total superoxide dimutase (T-SOD), peroxidase (POD) as well as catalase (CAT) activities, and their mRNA expressions in the head kidney of fish in the ß-glucan groups generally increased to higher levels after infection, and more quickly, compared with in the control group. Serum lysozyme (LSZ) and its expression in the head kidney in ß-glucan groups reached a higher peak earlier than in the control group. Serum glutamic oxalacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) levels in the ß-glucan groups were significantly lower than in the control group (P ≤ 0.05). The peak of heat shock protein 70 (HSP70) expression in the 0.2% ß-glucan group was higher and occurred earlier than in other groups (P ≤ 0.05). These results confirm that 0.1% and 0.2% dietary ß-glucan are beneficial for promoting growth in rainbow trout and enhancing resistance against A. salmonicida. Furthermore, ß-glucan could play an important role in regulating stress- and immune-related factors in rainbow trout to more quickly fight against bacterial infection. [Mh] Termos MeSH primário: Resistência à Doença Doenças dos Peixes/imunologia Infecções por Bactérias Gram-Negativas/veterinária Imunidade Inata Oncorhynchus mykiss beta-Glucanas/imunologia [Mh] Termos MeSH secundário: Adjuvantes Imunológicos/administração & dosagem Adjuvantes Imunológicos/fisiologia Aeromonas salmonicida/fisiologia Ração Animal/análise Animais Dieta/veterinária Suplementos Nutricionais/análise Relação Dose-Resposta a Droga Doenças dos Peixes/microbiologia Proteínas de Peixes/genética Proteínas de Peixes/metabolismo Infecções por Bactérias Gram-Negativas/imunologia Infecções por Bactérias Gram-Negativas/microbiologia Rim Cefálico/imunologia Rim Cefálico/metabolismo Oncorhynchus mykiss/crescimento & desenvolvimento Oncorhynchus mykiss/imunologia Distribuição Aleatória beta-Glucanas/administração & dosagem [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Adjuvants, Immunologic); 0 (Fish Proteins); 0 (beta-Glucans) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170629 [Lr] Data última revisão: 170629 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170314 [St] Status: MEDLINE

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[PMID]: 28271507 [Au] Autor: Li P; Zhou L; Wei S; Yang M; Ni S; Yu Y; Cai J; Qin Q [Ad] Endereço: Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry, Guangxi Academy of Sciences, Nanning, 530007, China. [Ti] Título: Establishment and characterization of a cell line from the head kidney of golden pompano Trachinotus ovatus and its application in toxicology and virus susceptibility. [So] Source: J Fish Biol;90(5):1944-1959, 2017 May. [Is] ISSN: 1095-8649 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: A cell line derived from the head kidney of golden pompano Trachinotus ovatus (TOHK) was established and characterized in this study. The TOHK cells grew most rapidly at 28° C and the optimum foetal bovine serum concentration in L-15 medium was 10%. The TOHK cells have a diploid chromosome number of 2N = 54. The transfection efficiency of TOHK cells was 7·5% at the 15th passage and 72% at the 40th passage. The transfection efficiency in TOHK cells was high, so these cells are suitable for foreign gene expression. The cytotoxic effects of heavy metals and extracellular products from Vibrio anguillarum and Vibrio alginolyticus were demonstrated in TOHK cells, so this TOHK cell line could also be applied in environmental monitoring of heavy metals and pathogenic bacteria. TOHK cell line showed high virus susceptibility, such as grouper nervous necrosis virus (GNNV) and Singapore grouper iridovirus (SGIV). Then, TOHK cell line could be used for the study of viral pathogenesis and the development of antiviral strategies. [Mh] Termos MeSH primário: Linhagem Celular Rim Cefálico/citologia Perciformes/fisiologia [Mh] Termos MeSH secundário: Animais Suscetibilidade a Doenças/virologia Monitoramento Ambiental/métodos Perciformes/genética Perciformes/virologia Transfecção [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171011 [Lr] Data última revisão: 171011 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170309 [St] Status: MEDLINE [do] DOI: 10.1111/jfb.13277

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[PMID]: 28262634 [Au] Autor: Lampe EO; Tandberg JI; Rishovd AL; Winther-Larsen HC [Ad] Endereço: Center for Integrative Microbiology and Evolution, Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, 0316 Oslo, Norway. [Ti] Título: Francisella noatunensis ssp. noatunensis iglC deletion mutant protects adult zebrafish challenged with acute mortality dose of wild-type strain. [So] Source: Dis Aquat Organ;123(2):123-140, 2017 03 06. [Is] ISSN: 0177-5103 [Cp] País de publicação: Germany [La] Idioma: eng [Ab] Resumo: The intracellular fish pathogen Francisella noatunensis remains an unsolved problem for aquaculture worldwide and an efficient vaccine is needed. In Francisella sp., IglC is an important virulence factor necessary for intracellular growth and escape from phagolysosomes. Deletion of the intracellular growth locus C (iglC) in Francisella sp. causes attenuation, but vaccine potential has only been attributed to ΔiglC from Francisella noatunensis ssp. orientalis, a warm-water fish pathogen. A ΔiglC mutant was constructed in the cold-water fish pathogen F. noatunensis ssp. noatunensis (Fnn), which causes francisellosis in Atlantic cod; the mutant was assessed in primary head kidney leucocytes from Atlantic cod. Fluorescence microscopy revealed reduced growth, while qPCR revealed an initial increase followed by a reduction in mutant genomes. Mutant-infected cod leucocytes presented higher interleukin 1 beta (il1ß) and interleukin 8 (il8) transcription than wild-type (WT)-infected cells. Two doses of mutant and WT were tested in an adult zebrafish model whereupon 3 × 109 CFU caused acute disease and 3 × 107 CFU caused low mortality regardless of strain. However, splenomegaly developed only in the WT-infected zebrafish. Immunization with 7 × 106 CFU of Fnn ΔiglC protected zebrafish against challenge with a lethal dose of Fnn WT, and bacterial load was minimized within 28 d. Immunized fish had lower interleukin 6 (il6) and il8 transcription in kidney and prolonged interferon-gamma (ifng) transcription in spleens after challenge compared with non-immunized fish. Our data suggest an immunogenic potential of Fnn ΔiglC and indicate important cytokines associated with francisellosis pathogenesis and protection. [Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo Francisella/patogenicidade Infecções por Bactérias Gram-Negativas/veterinária Peixe-Zebra [Mh] Termos MeSH secundário: Animais Proteínas de Bactérias/genética Citocinas/genética Citocinas/metabolismo Francisella/classificação Francisella/genética Gadiformes/fisiologia Deleção de Genes Regulação da Expressão Gênica/imunologia Regulação da Expressão Gênica/fisiologia Infecções por Bactérias Gram-Negativas/microbiologia Infecções por Bactérias Gram-Negativas/mortalidade Rim Cefálico/citologia Leucócitos/microbiologia Regulação para Cima Virulência [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Bacterial Proteins); 0 (Cytokines) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170629 [Lr] Data última revisão: 170629 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170307 [St] Status: MEDLINE [do] DOI: 10.3354/dao03087

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[PMID]: 28260650 [Au] Autor: Xing J; Ma J; Tang X; Sheng X; Zhan W [Ad] Endereço: Laboratory of Pathology and Immunology of Aquatic Animals,KLMME, Ocean University of China, Qingdao 266003, PR China. [Ti] Título: Characterizations of CD4-1, CD4-2 and CD8ß T cell subpopulations in peripheral blood leucocytes, spleen and head kidney of Japanese flounder (Paralichthys olivaceus). [So] Source: Mol Immunol;85:155-165, 2017 May. [Is] ISSN: 1872-9142 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: In the previous study, antibodies against CD3 molecule have been produced and were used in labeling T cells in Japanese flounder (Paralichthys olivaceus). In this paper, CD4 and CD8 lymphocytes subpopulations in peripheral blood leucocytes (PBL), spleen and head kidney of flounder were investigated. The flounder CD4-1, CD4-2 and CD8ß recombinant proteins and their antibodies (Abs) were produced, then the cross-reactivity of the Abs to CD4-1, CD4-2 and CD8ß was detected by Western blotting, respectively, and the reactions of Abs to PBL were analyzed by immunofluorescence staining (IFS) and flow cytometry (FCM). CD4-1 /CD3 , CD4-2 /CD3 , and CD8ß /CD3 lymphocytes in PBL, spleen and head kidney were observed by double IFS, then their proportions were analyzed using two-color FCM, respectively. Further, CD4-1/CD8ß, CD4-2/CD8ß, or CD4-1/CD4-2 lymphocytes were analyzed using double-IFS and two-color FCM. Finally, CD4-1 , CD4-2 , and CD8ß lymphocytes in spleen and head kidney were observed by immunohistochemistry. The results showed that the Abs were specific for CD4-1, CD4-2 and CD8ß molecules, respectively. The proportions of CD4-1 /CD3 , CD4-2 /CD3 , and CD8ß /CD3 lymphocytes were 6.7±2.0%, 8.6±2.8%, 2.1±1.3% in PBL; 13.6±3.6%, 15.6±5.2%, 2.8±1.4% in spleen; 20.0±4.6%, 20.5±4.6%, 3.2±1.5% in head kidney, respectively. Most CD4 and CD8 cell subpopulations belonged to CD3 cells; there were no cross-reactivity between CD4 and CD8 cells. CD4-1 /CD4-2 , CD4-1 /CD4-2 , and CD4-1 /CD4-2 cells presented different proportions in PBL, spleen and head kidney, among them, CD4-1 /CD4-2 cell is the majority of CD4T cell subpopulation. [Mh] Termos MeSH primário: Linguado/imunologia Rim Cefálico/imunologia Leucócitos/imunologia Baço/imunologia Subpopulações de Linfócitos T/imunologia [Mh] Termos MeSH secundário: Animais Western Blotting Linfócitos T CD4-Positivos/citologia Linfócitos T CD4-Positivos/imunologia Linfócitos T CD8-Positivos/citologia Linfócitos T CD8-Positivos/imunologia Citometria de Fluxo Imunofluorescência Rim Cefálico/citologia Leucócitos/citologia Baço/citologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170915 [Lr] Data última revisão: 170915 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170307 [St] Status: MEDLINE

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[PMID]: 28257924 [Au] Autor: Shirmohammadi M; Salamat N; Ronagh MT; Movahedinia A; Hamidian G [Ad] Endereço: Department of Marine Biology, Faculty of Marine Science, Khorramshahr University of Marine Science and Technology, Khorramshahr City, Khuzestan Province, Iran. Electronic address: shirmohammadim@yahoo.com. [Ti] Título: Assessment of immune status of yellowfin seabream (Acanthopagrus latus) during short term exposure to phenanthrene. [So] Source: Comp Biochem Physiol C Toxicol Pharmacol;195:78-90, 2017 May. [Is] ISSN: 1532-0456 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The aim of the present investigation was to assess the immune status in yellowfin seabream (Acanthopagrus latus) exposed to different concentrations of phenanthrene (Phe) for 14days. In addition, the Phe accumulation in the fish muscle was measured during the experiment. Fish were injected with different concentrations (0, 2, 20 and 40mg/kg) of Phe and samples were taken from tissue and blood of fish 1, 4, 7 and 14days after injection. Exposure of fish to Phe caused a significant decrease in white blood cells, C3 and C4 levels, lysosomal membrane stability, lysozyme activity after 4days and antibacterial activity after 7days of the experiment. In contrast, cortisol level significantly increased after 4days. The concentration of Phe in fish muscle increased rapidly after 4days. The main tissue changes observed in the head kidney including increase in melanomacrophage centers (MMCs), empty spaces between cells and hemorrhage. The degree of tissue changes ranged from normal to moderate in Phe-treated fish. The size and number of MMCs in treated fish were significantly higher than control. In conclusion, Phe toxicity in yellowfin seabream can induce increased cortisol level, tissue changes and immune suppression. [Mh] Termos MeSH primário: Sistema Imunitário/efeitos dos fármacos Fenantrenos/toxicidade Dourada/imunologia Poluentes Químicos da Água/toxicidade [Mh] Termos MeSH secundário: Animais Bactérias/imunologia Complemento C3/metabolismo Complemento C4/metabolismo Resistência à Doença/imunologia Relação Dose-Resposta a Droga Rim Cefálico/efeitos dos fármacos Rim Cefálico/imunologia Rim Cefálico/metabolismo Hidrocortisona/sangue Contagem de Leucócitos Leucócitos/efeitos dos fármacos Leucócitos/metabolismo Lisossomos/efeitos dos fármacos Lisossomos/metabolismo Muramidase/metabolismo Músculos/efeitos dos fármacos Músculos/imunologia Músculos/metabolismo Fenantrenos/farmacocinética Dourada/metabolismo Fatores de Tempo Poluentes Químicos da Água/farmacocinética [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Complement C3); 0 (Complement C4); 0 (Phenanthrenes); 0 (Water Pollutants, Chemical); 448J8E5BST (phenanthrene); EC 3.2.1.17 (Muramidase); WI4X0X7BPJ (Hydrocortisone) [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 170330 [Lr] Data última revisão: 170330 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170305 [St] Status: MEDLINE

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MEDLINE

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[PMID]: 28249227 [Au] Autor: Giraudo M; Douville M; Letcher RJ; Houde M [Ad] Endereço: Environment and Climate Change Canada, Aquatic Contaminants Research Division, 105 McGill Street, Montreal, QC, H2Y 2E7 Canada. Electronic address: maeva.giraudo@gmail.com. [Ti] Título: Effects of food-borne exposure of juvenile rainbow trout (Oncorhynchus mykiss) to emerging brominated flame retardants 1,2-bis(2,4,6-tribromophenoxy)ethane and 2-ethylhexyl-2,3,4,5-tetrabromobenzoate. [So] Source: Aquat Toxicol;186:40-49, 2017 May. [Is] ISSN: 1879-1514 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Brominated flame retardants (BFRs) represent a large group of chemicals used in a variety of household and commercial products to prevent fire propagation. The environmental persistence and toxicity of some of the most widely used BFRs has resulted in a progressive ban worldwide and the development of novel BFRs for which the knowledge on environmental health impacts remains limited. The objectives of this study were to evaluate the effects of two emerging BFRs, 1,2-bis(2,4,6-tribromophenoxy)ethane (BTBPE) and 2-ethylhexyl-2,3,4,5-tetrabromobenzoate (EH-TBB), in diet exposed juvenile rainbow trout (Oncorhynchus mykiss). Both compounds were detected in fish carcasses at 76% and 2% of the daily dosage of BTBPE and EH-TBB, respectively, indicating accumulation of BTBPE and by contrast extensive depuration/metabolism of EH-TBB. Liver gene transcription analysis using RNA-sequencing indicated that the chronic 28-d dietary exposure of trout to EH-TBB down-regulated one single gene related to endocrine-mediated processes, whereas BTBPE impacted the transcription of 33 genes, including genes involved in the immune response, reproduction, and oxidative stress. Additional analysis using qRT-PCR after 48-h and 28-d of exposure confirmed the impact of BTBPE on immune related genes in the liver (apolipoprotein A-I, lysozyme) and the head-kidney (complement c3-4). However, the activity of lysozymes measured at the protein level did not reflect transcriptomic results. Overall, results suggested an impact on immune-related gene transcription in BTBPE exposed fish, as well as oxidative stress and endocrine disruption potentials. [Mh] Termos MeSH primário: Benzoatos/toxicidade Bromobenzenos/toxicidade Exposição Ambiental/análise Retardadores de Chama/toxicidade Alimentos Oncorhynchus mykiss/metabolismo [Mh] Termos MeSH secundário: Animais Benzoatos/química Bromobenzenos/química Dieta Rim Cefálico/efeitos dos fármacos Rim Cefálico/metabolismo Fígado/efeitos dos fármacos Fígado/metabolismo Anotação de Sequência Molecular Peso Molecular Muramidase/metabolismo Oncorhynchus mykiss/crescimento & desenvolvimento RNA Mensageiro/genética RNA Mensageiro/metabolismo Reação em Cadeia da Polimerase em Tempo Real Análise de Sequência de RNA Transcrição Genética/efeitos dos fármacos Transcriptoma/efeitos dos fármacos Transcriptoma/genética Poluentes Químicos da Água/química Poluentes Químicos da Água/toxicidade [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (2-ethylhexyl 2,3,4,5-tetrabromobenzoate); 0 (Benzoates); 0 (Bromobenzenes); 0 (Flame Retardants); 0 (RNA, Messenger); 0 (Water Pollutants, Chemical); 34I00D6RNM (1,2-bis(2,4,6-tribromophenoxy)ethane); EC 3.2.1.17 (Muramidase) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170302 [St] Status: MEDLINE

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