Base de dados : MEDLINE
Pesquisa : A13.463 [Categoria DeCS]
Referências encontradas : 936 [refinar]
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[PMID]:29175396
[Au] Autor:Wang F; Liu F; Chen W; Xu R; Wang W
[Ad] Endereço:School of Biological Science, Luoyang Normal University, Luoyang, 471022, China; Cold Water Fish Breeding Engineering Technology Research Center of Henan Province, Luoyang, 471022, China. Electronic address: wangfan7677@163.com.
[Ti] Título:Effects of triclosan (TCS) on hormonal balance and genes of hypothalamus-pituitary- gonad axis of juvenile male Yellow River carp (Cyprinus carpio).
[So] Source:Chemosphere;193:695-701, 2018 Feb.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Triclosan (TCS) is a broad spectrum antimicrobial agent which has been widely dispersed and determinated in the aquatic environment. However, the effects of TCS on reproductive endocrine in male fish are poorly understood. In this study, male Yellow River carp (Cyprinus carpio) were exposed to 0, 1/5, 1/10 and 1/20 LC (96 h LC of TCS to carp) TCS under semi-static conditions for 42 d. Vitellogenin (Vtg), 17ß-estradiol (E ), testosterone(T), gonadotropin (GtH), and gonadotropin-releasing hormone (GnRH) levels were measured by enzyme-linked immunosorbent assay (ELISA). Meanwhile, we also examined the mRNA expressions of aromatase, GtHs-ß, GnRH, estrogen receptor (Er), and androgen receptor (Ar) by quantitative Real-time Polymerase Chain Reaction (qRT-PCR). TCS induced Vtg levels of hepatopancreas, E levels of serum, and inhibited Ar and Er mRNA levels, suggesting that the induction of Vtg production by TCS was indirectly caused by non-Er pathways. TCS-induced Vtg levels by interfering with the reproductive axis at plenty of latent loci of male carps: (a) TCS exposure increased the aromatase mRNA expression of hypothalamus and gonad aromatase, consequently increasing serum concentrations of E to induce Vtg in hepatopancreas; (b) TCS treatment changed GtH-ß and GnRH mRNA expression and secretion, causing the disturbance of reproductive endocrine; (c) TCS exposure decreased Ar mRNA levels, indicating potential Ar-mediated antiandrogen action. These mechanisms showed that TCS may induce Vtg production in male carp by non-Er-mediated pathways.
[Mh] Termos MeSH primário: Carpas/metabolismo
Triclosan/toxicidade
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Anti-Infecciosos/toxicidade
Aromatase/genética
Sistema Endócrino/efeitos dos fármacos
Ensaio de Imunoadsorção Enzimática
Estradiol/análise
Hormônio Liberador de Gonadotropina/análise
Hormônio Liberador de Gonadotropina/genética
Gônadas/enzimologia
Gônadas/metabolismo
Hepatopâncreas/metabolismo
Hormônios/metabolismo
Hipotálamo/metabolismo
Masculino
Hipófise/metabolismo
RNA Mensageiro/análise
Reação em Cadeia da Polimerase em Tempo Real
Receptores Estrogênicos/genética
Reprodução/efeitos dos fármacos
Testosterona/análise
Vitelogeninas/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Hormones); 0 (RNA, Messenger); 0 (Receptors, Estrogen); 0 (Vitellogenins); 0 (Water Pollutants, Chemical); 33515-09-2 (Gonadotropin-Releasing Hormone); 3XMK78S47O (Testosterone); 4NM5039Y5X (Triclosan); 4TI98Z838E (Estradiol); EC 1.14.14.1 (Aromatase); EC 1.14.14.1 (CYP19A1 protein, human)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE


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[PMID]:29339067
[Au] Autor:Sun Y; Zhang J; Xiang J
[Ad] Endereço:College of Life Sciences, Hebei University, Baoding, Hebei 071002, China; College of Marine Life and Fisheries, Huaihai Institute of Technology, 59 Cangwu Road, Lianyungang 222005, China.
[Ti] Título:Molecular characterization and function of ß-N-acetylglucosaminidase from ridgetail white prawn Exopalaemon carinicauda.
[So] Source:Gene;648:12-20, 2018 Mar 30.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Chitin degradation is catalyzed by a two-component chitinolytic enzyme system, chitinase and ß-N-acetylglucosaminidase (NAGase). In this paper, the full-length cDNA sequence encoding NAGase (EcNAG) was obtained from Exopalaemon carinicauda. The deduced amino acid sequence of EcNAG open reading frame (ORF) contained one Glycohydro_20b2 domain and one Glyco_hydro_20 domain. Based on the cDNA sequence, the genomic structure of EcNAG was characterized and it was composed of six exons and five introns. EcNAG mRNA majorly expressed in the hepatopancreas and epidermis. During the molting stages, EcNAG mRNA expression was well-regulated and its expression reached the highest level at the molting stage E. In addition, EcNAG was recombinant expressed in Pichia pastoris and the partial enzymatic characterization of recombinant EcNAG was confirmed. After being challenged with Vibrio parahaemolyticus and Aeromonas hydrophila, the expression of EcNAG was up-regulated significantly at 6 h and reached the peak at 12 h. And then, the expression began to down-regulated and came to the normal level at 72 h. It is helpful to research the relationship between the molt-related hormones and chitinlytic enzymes.
[Mh] Termos MeSH primário: Acetilglucosaminidase/genética
Proteínas de Artrópodes/genética
Muda/genética
Palaemonidae/genética
[Mh] Termos MeSH secundário: Acetilglucosaminidase/classificação
Acetilglucosaminidase/metabolismo
Aeromonas hydrophila/fisiologia
Sequência de Aminoácidos
Animais
Proteínas de Artrópodes/classificação
Proteínas de Artrópodes/metabolismo
Sequência de Bases
Epiderme/crescimento & desenvolvimento
Epiderme/metabolismo
Epiderme/microbiologia
Doenças dos Peixes/genética
Doenças dos Peixes/microbiologia
Perfilação da Expressão Gênica/métodos
Regulação da Expressão Gênica no Desenvolvimento
Regulação Enzimológica da Expressão Gênica
Hepatopâncreas/crescimento & desenvolvimento
Hepatopâncreas/metabolismo
Hepatopâncreas/microbiologia
Palaemonidae/crescimento & desenvolvimento
Palaemonidae/microbiologia
Filogenia
Homologia de Sequência de Aminoácidos
Vibrio parahaemolyticus/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arthropod Proteins); EC 3.2.1.52 (Acetylglucosaminidase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180118
[St] Status:MEDLINE


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[PMID]:28992477
[Au] Autor:Nuñez-Hernandez DM; Felix-Portillo M; Peregrino-Uriarte AB; Yepiz-Plascencia G
[Ad] Endereço:Centro de Investigación en Alimentación y Desarrollo, A.C. P.O. Box 1735. Carretera a Ejido La Victoria Km. 0.6 Hermosillo, Sonora, 83304, Mexico.
[Ti] Título:Cell cycle regulation and apoptosis mediated by p53 in response to hypoxia in hepatopancreas of the white shrimp Litopenaeus vannamei.
[So] Source:Chemosphere;190:253-259, 2018 Jan.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Although hypoxic aquatic environments cause negative effects on shrimp, these animals can withstand somewhat hypoxia, but the cellular mechanisms underlying this capacity are still poorly understood. In humans, mild hypoxia causes the induction of many proteins to allow cell survival. In contrast, apoptosis is induced during severe hypoxia leading to cell death. p53 is a key transcription factor that determines cells fate towards cell cycle arrest or induction of apoptosis in humans. The aim of this work was to study the role of p53 in cell cycle regulation and apoptosis in response to hypoxia in hepatopancreas of the white shrimp Litopenaeus vannamei. p53 was silenced by RNAi and afterwards the shrimp were exposed to hypoxia. Cdk-2 was used as indicator of cell cycle progression while caspase-3 expression and caspase activity were analyzed as indicators of apoptosis. p53 levels in hepatopancreas were significantly higher at 48 h after hypoxic treatment. Increased expression levels of Cdk-2 were found in p53-silenced shrimp after 24 and 48 h in the normoxic treatments as well as 48 h after hypoxia, indicating a possible role of p53 in cell cycle regulation. In response to hypoxia, unsilenced shrimp showed an increase in caspase-3 expression levels, however an increase was also observed in caspase activity at 24 h of normoxic conditions in p53-silenced shrimps. Taken together these results indicate the involvement of p53 in regulation of cell cycle and apoptosis in the white shrimp in response to hypoxia.
[Mh] Termos MeSH primário: Apoptose
Pontos de Checagem do Ciclo Celular
Hepatopâncreas/metabolismo
Hipóxia/fisiopatologia
Penaeidae/metabolismo
Proteína Supressora de Tumor p53/fisiologia
[Mh] Termos MeSH secundário: Animais
Caspase 3/análise
Caspase 3/metabolismo
Hipóxia/metabolismo
Penaeidae/anatomia & histologia
Proteína Supressora de Tumor p53/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Tumor Suppressor Protein p53); EC 3.4.22.- (Caspase 3)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171010
[St] Status:MEDLINE


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[PMID]:29322345
[Au] Autor:Galvão RCF; Holanda IBB; De Carvalho DP; Almeida R; Souza CMM; Lacerda LD; Bastos WR
[Ad] Endereço:Instituto Federal de Educação Ciência e Tecnologia de Rondônia-IFRO, Rodovia BR-435 Km 63 Zona Rural, Caixa Postal 51, Colorado do Oeste, RO, 76993-000, Brazil.
[Ti] Título:Freshwater shrimps (Macrobrachium depressimanum and Macrobrachium jelskii) as biomonitors of Hg availability in the Madeira River Basin, Western Amazon.
[So] Source:Environ Monit Assess;190(2):77, 2018 Jan 10.
[Is] ISSN:1573-2959
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Total mercury (THg) concentrations measured in two freshwater shrimp species (Macrobrachium depressimanum and Macrobrachium jelskii) showed a relationship with the location of artisanal and small-scale gold mining (ASGM) from the Madeira River Basin, Western Amazon. Between August 2009 and May 2010, 212 shrimp samples were collected in the confluence of the Madeira River with three of its tributaries (Western Amazon). THg concentration was quantified in the exoskeleton, hepatopancreas and muscle tissue of the shrimps by cold vapor atomic absorption spectrophotometry. There were no significant differences between the two shrimp species when samples came from the Madeira River, but Hg concentrations were significantly lower in a tributary outside the influence of the gold mining area. Average THg concentrations were higher in the hepatopancreas (up to 160.0 ng g ) and lower in the exoskeleton and muscle tissue (10.0-35.0 ng g and < 0.9-42.0 ng g , respectively). Freshwater shrimps from the Madeira River respond to local environmental levels of Hg and can be considered as biomonitors for environmental Hg at this spatial scale. These organisms are important for moving Hg up food webs including those that harbor economic significant fish species and thus enhancing human exposure.
[Mh] Termos MeSH primário: Ouro
Mercúrio/análise
Mineração
Palaemonidae
Poluentes Químicos da Água/análise
[Mh] Termos MeSH secundário: Exoesqueleto/química
Animais
Brasil
Monitoramento Ambiental
Hepatopâncreas/química
Músculos/química
Rios
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Water Pollutants, Chemical); 7440-57-5 (Gold); FXS1BY2PGL (Mercury)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.1007/s10661-018-6460-6


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[PMID]:29235319
[Au] Autor:Toptikov VA; Totsky VN; Alieksieieva TG; Kovtun OA
[Ti] Título:Hydrolytic enzymes expressivity in different parts of the Rapana digestive system.
[So] Source:Ukr Biochem J;88(3):5-17, 2016 May-Jun.
[Is] ISSN:2409-4943
[Cp] País de publicação:Ukraine
[La] Idioma:eng
[Ab] Resumo:The relevance of comprehensive studies of the Rapana vital functions is determined by its considerab­le negative impact on the ecosystem of the Black Sea. The aim of the work was to find out the polymorphism and activity of the main hydrolases in the different parts of the digestive system of Rapana. Hydrolases (proteases, amylases, esterases, lipases and phosphatases) in glandular structures of the Rapana digestive system were studied by electrophoresis. It was found that different sets of hydrolytic enzymes are functioning in certain parts of the Rapana digestive tract. The gland of Leiblein and hepatopancreas played the most important role in the digestion of food components. The salivary glands had the significant influence on proteolysis.
[Mh] Termos MeSH primário: Mucosa Gástrica/enzimologia
Gastrópodes/enzimologia
Expressão Gênica
Hepatopâncreas/enzimologia
Espécies Introduzidas
Glândulas Salivares/enzimologia
[Mh] Termos MeSH secundário: Amilases/classificação
Amilases/genética
Amilases/metabolismo
Animais
Mar Negro
Ensaios Enzimáticos
Esterases/classificação
Esterases/genética
Esterases/metabolismo
Gastrópodes/genética
Lipase/classificação
Lipase/genética
Lipase/metabolismo
Peptídeo Hidrolases/classificação
Peptídeo Hidrolases/genética
Peptídeo Hidrolases/metabolismo
Monoéster Fosfórico Hidrolases/classificação
Monoéster Fosfórico Hidrolases/genética
Monoéster Fosfórico Hidrolases/metabolismo
Comportamento Predatório/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.1.- (Esterases); EC 3.1.1.3 (Lipase); EC 3.1.3.2 (Phosphoric Monoester Hydrolases); EC 3.2.1.- (Amylases); EC 3.4.- (Peptide Hydrolases)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.15407/ubj88.03.005


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[PMID]:29180238
[Au] Autor:Asaro A; Paggi RA; Del Valle JC; López Mañanes AA
[Ad] Endereço:Instituto de Investigaciones Marinas y Costeras (IIMyC), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Mar del Plata, Argentina.
[Ti] Título:Glucose homeostasis in the euryhaline crab Cytograpsus angulatus: Effects of the salinity in the amylase, maltase and sucrase activities in the hepatopancreas and in the carbohydrate reserves in different tissues.
[So] Source:Comp Biochem Physiol B Biochem Mol Biol;216:39-47, 2018 Feb.
[Is] ISSN:1879-1107
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We studied the existence, biochemical characteristics and response to different environmental salinities of amylase, maltase and sucrase activity in the intertidal euryhaline crab Cyrtograpsus angulatus (Dana, 1852) along with the response to distinct salinities of glycogen and free glucose content in storage organs. Amylase, maltase and sucrase activities were kept over a broad range of pH and temperature and exhibited Michaelis-Menten kinetics. Zymography showed the existence of two amylase forms in crabs exposed to 35 (osmoconformation) and low (6-10psu; hyper-regulation) or high (40psu) (hypo-regulation) salinities. Carbohydrases activity in the hepatopancreas and glycemia were not affected in crab exposed to different environmental salinities. In 6 and 40psu, the glycogen content in anterior gills was lower than in 35psu. In 6, 10 and 40psu, glycogen concentration in hepatopancreas, muscle and posterior gills were similar to that in 35psu. Free glucose concentration in chela muscle was higher in 6 and 40psu than in 35psu. The existence and biochemical characteristics of carbohydrases activity and the adjustments in concentration of glycogen in anterior gills and free glucose in chela muscle suggests the ability to perform complete hydrolysis of glycogenic substrates and to keep glucose homeostasis in relation to acclimation to different salinity conditions.
[Mh] Termos MeSH primário: Amilases/metabolismo
Proteínas de Artrópodes/metabolismo
Braquiúros/metabolismo
Glucose/metabolismo
Hepatopâncreas/metabolismo
Salinidade
Sacarase/metabolismo
alfa-Glucosidases/metabolismo
[Mh] Termos MeSH secundário: Animais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arthropod Proteins); EC 3.2.1.- (Amylases); EC 3.2.1.20 (alpha-Glucosidases); EC 3.2.1.48 (Sucrase); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180102
[Lr] Data última revisão:
180102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE


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[PMID]:28980889
[Au] Autor:Tian JJ; Lei CX; Ji H; Kaneko G; Zhou JS; Yu HB; Li Y; Yu EM; Xie J
[Ad] Endereço:1College of Animal Science and Technology,Northwest A&F University,Yangling 712100,People's Republic of China.
[Ti] Título:Comparative analysis of effects of dietary arachidonic acid and EPA on growth, tissue fatty acid composition, antioxidant response and lipid metabolism in juvenile grass carp, Ctenopharyngodon idellus.
[So] Source:Br J Nutr;118(6):411-422, 2017 Sep.
[Is] ISSN:1475-2662
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Four isonitrogenous and isoenergetic purified diets containing free arachidonic acid (ARA) or EPA (control group), 0·30 % ARA, 0·30 % EPA and 0·30 % ARA+EPA (equivalent) were designed to feed juvenile grass carp (10·21 (sd 0·10) g) for 10 weeks. Only the EPA group presented better growth performance compared with the control group (P<0·05). Dietary ARA and EPA were incorporated into polar lipids more than non-polar lipids in hepatopancreas but not intraperitoneal fat (IPF) tissue. Fish fed ARA and EPA showed an increase of serum superoxide dismutase and catalase activities, and decrease of glutathione peroxidase activity and malondialdehyde contents (P<0·05). The hepatopancreatic TAG levels decreased both in ARA and EPA groups (P<0·05), accompanied by the decrease of lipoprotein lipase (LPL) activity in the ARA group (P<0·05). Fatty acid synthase (FAS), diacylglycerol O-acyltransferase and apoE gene expression in the hepatopancreas decreased in fish fed ARA and EPA, but only the ARA group exhibited increased mRNA level of adipose TAG lipase (ATGL) (P<0·05). Decreased IPF index and adipocyte sizes were found in the ARA group (P<0·05). Meanwhile, the ARA group showed decreased expression levels of adipogenic genes CCAAT enhancer-binding protein α, LPL and FAS, and increased levels of the lipid catabolic genes PPAR α, ATGL, hormone-sensitive lipase and carnitine palmitoyltransferase 1 (CPT-1) in IPF, whereas the EPA group only increased PPAR α and CPT-1 mRNA expression and showed less levels than the ARA group. Overall, dietary EPA is beneficial to the growth performance, whereas ARA is more potent in inducing lipolysis and inhibiting adipogenesis, especially in IPF. Meanwhile, dietary ARA and EPA showed the similar preference in esterification and the improvement in antioxidant response.
[Mh] Termos MeSH primário: Antioxidantes/metabolismo
Ácido Araquidônico/administração & dosagem
Composição Corporal
Carpas/fisiologia
Ácido Eicosapentaenoico/administração & dosagem
Metabolismo dos Lipídeos
[Mh] Termos MeSH secundário: Adipócitos/efeitos dos fármacos
Adipócitos/metabolismo
Adipogenia/efeitos dos fármacos
Adipogenia/genética
Ração Animal/análise
Animais
Proteína alfa Estimuladora de Ligação a CCAAT/genética
Proteína alfa Estimuladora de Ligação a CCAAT/metabolismo
Carnitina O-Palmitoiltransferase/genética
Carnitina O-Palmitoiltransferase/metabolismo
Dieta/veterinária
Glutationa Peroxidase/sangue
Hepatopâncreas/efeitos dos fármacos
Hepatopâncreas/metabolismo
Lipase Lipoproteica/sangue
Malondialdeído/sangue
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Superóxido Dismutase/sangue
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (CCAAT-Enhancer-Binding Protein-alpha); 0 (RNA, Messenger); 27YG812J1I (Arachidonic Acid); 4Y8F71G49Q (Malondialdehyde); AAN7QOV9EA (Eicosapentaenoic Acid); EC 1.11.1.9 (Glutathione Peroxidase); EC 1.15.1.1 (Superoxide Dismutase); EC 2.3.1.21 (Carnitine O-Palmitoyltransferase); EC 3.1.1.34 (Lipoprotein Lipase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171011
[Lr] Data última revisão:
171011
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171006
[St] Status:MEDLINE
[do] DOI:10.1017/S000711451700215X


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[PMID]:28951248
[Au] Autor:Li R; Zhu LN; Ren LQ; Weng JY; Sun JS
[Ad] Endereço:Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Science, Tianjin Normal University, Tianjin, People's Republic of China.
[Ti] Título:Molecular cloning and characterization of glycogen synthase in Eriocheir sinensis.
[So] Source:Comp Biochem Physiol B Biochem Mol Biol;214:47-56, 2017 Dec.
[Is] ISSN:1879-1107
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Glycogen plays an important role in glucose and energy homeostasis at cellular and organismal levels. In glycogen synthesis, glycogen synthase (GS) is a rate-limiting enzyme catalysing the addition of α-1,4-linked glucose units from (UDP) -glucose to a nascent glycogen chain using glycogenin (GN) as a primer. While studies on mammalian liver GS (GYS2) are numerous, enzymes from crustaceans, which also use glycogen and glucose as their main energy source, have received less attention. In the present study, we amplified full-length GS cDNA from Eriocheir sinensis. Tissue expression profiling revealed the highest expression of GS in the hepatopancreas. During moulting, GS expression and activity declined, and glycogen levels in the hepatopancreas were reduced. Recombinant GS was expressed in Escherichia coli Rosetta (DE3), and induction at 37°C or 16°C yielded EsGS in insoluble inclusion bodies (EsGS-I) or in soluble form (EsGS-S), respectively. Enzyme activity was measured in a cell-free system containing glucose-6-phosphate (G6P), and both forms possessed glycosyltransferase activity, but refolded EsGS-I was more active. Enzyme activity of both GS and EsGS-I in the hepatopancreas was optimum at 25°C, which is coincident with the optimum growth temperature of Chinese mitten crab, and higher (37°C) or lower (16°C) temperatures resulted in lower enzyme activity. Taken together, the results suggest that GS may be important for maintaining normal physiological functions such as growth and reproduction.
[Mh] Termos MeSH primário: Proteínas de Artrópodes/genética
Braquiúros/enzimologia
Glicogênio Sintase/genética
Glicogênio/biossíntese
Hepatopâncreas/enzimologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Proteínas de Artrópodes/metabolismo
Sítios de Ligação
Braquiúros/genética
Braquiúros/crescimento & desenvolvimento
Clonagem Molecular
Escherichia coli/genética
Escherichia coli/metabolismo
Expressão Gênica
Glucose-6-Fosfato/metabolismo
Glucosiltransferases/metabolismo
Glicogênio Sintase/metabolismo
Glicoproteínas/metabolismo
Hepatopâncreas/crescimento & desenvolvimento
Corpos de Inclusão/química
Cinética
Muda/genética
Especificidade de Órgãos
Filogenia
Ligação Proteica
Domínios e Motivos de Interação entre Proteínas
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Alinhamento de Sequência
Homologia de Sequência de Aminoácidos
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arthropod Proteins); 0 (Glycoproteins); 0 (Recombinant Proteins); 0 (glycogenin); 56-73-5 (Glucose-6-Phosphate); 9005-79-2 (Glycogen); EC 2.4.1.- (Glucosyltransferases); EC 2.4.1.11 (Glycogen Synthase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170928
[St] Status:MEDLINE


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[PMID]:28918749
[Au] Autor:Wu M; Chen N; Huang CX; He Y; Zhao YZ; Chen XH; Chen XL; Wang HL
[Ad] Endereço:Ministry of Education, Huazhong Agricultural University, College of Fishery, Key Lab of Freshwater Animal Breeding, Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction, Wuhan, 430070, PR China. hbauwhl@hotmail.com.
[Ti] Título:Effect of Low Temperature on Globin Expression, Respiratory Metabolic Enzyme Activities, and Gill Structure of Litopenaeus vannamei.
[So] Source:Biochemistry (Mosc);82(7):844-851, 2017 Jul.
[Is] ISSN:1608-3040
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Low temperature frequently influences growth, development, and even survival of aquatic animals. In the present study, physiological and molecular responses to low temperature in Litopenaeus vannamei were investigated. The cDNA sequences of two oxygen-carrying proteins, cytoglobin (Cygb) and neuroglobin (Ngb), were isolated. Protein structure analysis revealed that both proteins share a globin superfamily domain. Real-time PCR analysis indicated that Cygb and Ngb mRNA levels gradually increased during decrease in temperatures from 25 to 15°C and then decreased at 10°C in muscle, brain, stomach, and heart, except for a continuing increase in gills, whereas they showed a different expression trend in the hepatopancreas. Hemocyanin concentration gradually reduced as the temperature decreased. Moreover, the activities of respiratory metabolic enzymes including lactate dehydrogenase (LDH) and succinate dehydrogenase (SDH) were measured, and it was found that LDH activity gradually increased while SDH activity decreased after low-temperature treatment. Finally, damage to gill structure at low temperature was also observed, and this intensified with further decrease in temperature. Taken together, these results show that low temperature has an adverse influence in L. vannamei, which contributes to systematic understanding of the adaptation mechanisms of shrimp at low temperature.
[Mh] Termos MeSH primário: Temperatura Baixa
Regulação da Expressão Gênica
Globinas/genética
Proteínas do Tecido Nervoso/genética
Penaeidae/anatomia & histologia
Penaeidae/fisiologia
[Mh] Termos MeSH secundário: Animais
Bases de Dados Factuais
Brânquias/anatomia & histologia
Brânquias/metabolismo
Globinas/química
Globinas/metabolismo
Hemocianinas/análise
Hepatopâncreas/metabolismo
L-Lactato Desidrogenase/genética
L-Lactato Desidrogenase/metabolismo
Proteínas do Tecido Nervoso/química
Proteínas do Tecido Nervoso/metabolismo
Penaeidae/enzimologia
Penaeidae/genética
RNA Mensageiro/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
Succinato Desidrogenase/genética
Succinato Desidrogenase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nerve Tissue Proteins); 0 (RNA, Messenger); 0 (cytoglobin); 0 (neuroglobin); 9004-22-2 (Globins); 9013-72-3 (Hemocyanins); EC 1.1.1.27 (L-Lactate Dehydrogenase); EC 1.3.99.1 (Succinate Dehydrogenase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170919
[St] Status:MEDLINE
[do] DOI:10.1134/S0006297917070100


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[PMID]:28910412
[Au] Autor:Yang Z; Zhang Y; Jiang Y; Zhu F; Zeng L; Wang Y; Lei X; Yao Y; Hou Y; Xu L; Xiong C; Yang X; Hu K
[Ad] Endereço:National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai, China.
[Ti] Título:Transcriptional responses in the hepatopancreas of Eriocheir sinensis exposed to deltamethrin.
[So] Source:PLoS One;12(9):e0184581, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Deltamethrin is an important pesticide widely used against ectoparasites. Deltamethrin contamination has resulted in a threat to the healthy breeding of the Chinese mitten crab, Eriocheir sinensis. In this study, we investigated transcriptional responses in the hepatopancreas of E. sinensis exposed to deltamethrin. We obtained 99,087,448, 89,086,478, and 100,117,958 raw sequence reads from control 1, control 2, and control 3 groups, and 92,094,972, 92,883,894, and 92,500,828 raw sequence reads from test 1, test 2, and test 3 groups, respectively. After filtering and quality checking of the raw sequence reads, our analysis yielded 79,228,354, 72,336,470, 81,859,826, 77,649,400, 77,194,276, and 75,697,016 clean reads with a mean length of 150 bp from the control and test groups. After deltamethrin treatment, a total of 160 and 167 genes were significantly upregulated and downregulated, respectively. Gene ontology terms "biological process," "cellular component," and "molecular function" were enriched with respect to cell killing, cellular process, other organism part, cell part, binding, and catalytic. Pathway analysis using the Kyoto Encyclopedia of Genes and Genomes showed that the metabolic pathways were significantly enriched. We found that the CYP450 enzyme system, carboxylesterase, glutathione-S-transferase, and material (including carbohydrate, lipid, protein, and other substances) metabolism played important roles in the metabolism of deltamethrin in the hepatopancreas of E. sinensis. This study revealed differentially expressed genes related to insecticide metabolism and detoxification in E. sinensis for the first time and will help in understanding the toxicity and molecular metabolic mechanisms of deltamethrin in E. sinensis.
[Mh] Termos MeSH primário: Braquiúros/genética
Perfilação da Expressão Gênica/métodos
Hepatopâncreas/efeitos dos fármacos
Nitrilos/farmacologia
Piretrinas/farmacologia
Análise de Sequência de RNA/métodos
[Mh] Termos MeSH secundário: Animais
Braquiúros/efeitos dos fármacos
Regulação da Expressão Gênica/efeitos dos fármacos
Ontologia Genética
Redes Reguladoras de Genes/efeitos dos fármacos
Sequenciamento de Nucleotídeos em Larga Escala/métodos
Masculino
Redes e Vias Metabólicas/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nitriles); 0 (Pyrethrins); 2JTS8R821G (decamethrin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170915
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184581



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