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[PMID]:29441913
[Ti] Título:Stimulation of bone regeneration with pigment epithelium-derived factor microparticles: evidence and .
[So] Source:Pharmazie;71(7):382-389, 2016 Jul 07.
[Is] ISSN:0031-7144
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The occurrence of bone defects can be due to a variety of factors not limited to bone fractures and tumours. Most diseased bone is removed and the patient fitted with prosthetics, prior to use of certain factors such as bone morphogenetic proteins (BMPs) to aid healing. Recently, the protein pigment epithelium-derived factor (PEDF) and the polysaccharide chitosan have been found to have promising effects on the regeneration of bone, with the major advantage of these agents being their safety to date. A study was performed to determine whether the combination of both chitosan and PEDF would enhance greater bone regeneration effects. Post-formulation, in silico tests (particle sizing and surface charge determination) were followed by several cell-based assays (microparticle cellular uptake, cytotoxicity, mitochondrial abundance, bone mineral formation, colony formation in matrigel, and colony formation in collagen I matrix), and finally in vivo testing where microparticles were injected periosteally in the hindlimb. Collectively, these findings support the idea that PEDF microencapsulated within chitosan promotes bone regeneration, and has potential for bone trauma management. Future studies will examine the ability of this promising bone regeneration microparticle to heal bone in disease states such as fracture and tumour-mediated osteolysis.
[Mh] Termos MeSH primário: Regeneração Óssea/efeitos dos fármacos
Proteínas do Olho/farmacologia
Fatores de Crescimento Neural/farmacologia
Serpinas/farmacologia
[Mh] Termos MeSH secundário: Animais
Densidade Óssea/efeitos dos fármacos
Cápsulas
Sobrevivência Celular/efeitos dos fármacos
Quitosana/farmacologia
Colágeno Tipo I/farmacologia
Ensaio de Unidades Formadoras de Colônias
Simulação por Computador
Composição de Medicamentos
Proteínas do Olho/administração & dosagem
Proteínas do Olho/metabolismo
Membro Posterior
Seres Humanos
Injeções
Masculino
Camundongos
Camundongos Endogâmicos BALB C
Mitocôndrias/efeitos dos fármacos
Fatores de Crescimento Neural/administração & dosagem
Fatores de Crescimento Neural/metabolismo
Tamanho da Partícula
Serpinas/administração & dosagem
Serpinas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Capsules); 0 (Collagen Type I); 0 (Eye Proteins); 0 (Nerve Growth Factors); 0 (Serpins); 0 (pigment epithelium-derived factor); 9012-76-4 (Chitosan)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180215
[St] Status:MEDLINE
[do] DOI:10.1691/ph.2016.6010


  2 / 20758 MEDLINE  
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[PMID]:29230999
[Au] Autor:Bai RF; Lü XJ; E XF; Yu TS; Liu R; Zhang HD
[Ad] Endereço:2011 Cooperative Innovation Center of Judicial Civilization, Beijing 100088, China.
[Ti] Título:[Comparison of the Skin and Skeletal Muscle Contusion in Rats Induced by Blunt Force with Different Heights].
[So] Source:Fa Yi Xue Za Zhi;33(1):1-5, 2017 Feb.
[Is] ISSN:1004-5619
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVES: To explore the differences in the repair process of skin and skeletal muscle after contusion caused by blunt force attack with different heights. METHODS: Three degrees of contusion were performed on SD rats' right hind limbs by a designed free-dropping device falling from 15, 30 and 50 cm heights, which as a main consideration factor for degree of injury. The repair process of skin and skeletal muscle at 6 h, 24 h, 3 d, 7 d and 13 d after contusion were observed using routine histological methods. RESULTS: Hematoma within skin and/or muscle was found in the rats' hind limbs after contusion with three different heights. The repair processes were similar at 24 h after contusion. However, with the increase of height, the display degree was more obvious. At 3 d after contusion, the RBC of the hemorrhagic region would be decomposed and elapsed in 15 cm contusion group, but for 30 cm contusion group, it delayed to 7 d. At 13 d after contusion, the similar result was found in 15 cm and 30 cm contusion groups, in contrast, the 50 cm contusion group was still in the proliferative phase. CONCLUSIONS: With the increase of height, the occurring rate of hematoma within skin and muscle at the same time increases, and the more serious histological appearance after contusion, including inflammation and proliferation, the longer healing process are observed. According to the results of present study and considering forensic application, the contusion model with 50 cm height (2.58 J/cm²ï¼‰ is recommended as the experimental animal model for the future study of wound age estimation on contusion.
[Mh] Termos MeSH primário: Contusões/patologia
Músculo Esquelético/lesões
Pele/lesões
Ferimentos não Penetrantes
[Mh] Termos MeSH secundário: Animais
Contusões/etiologia
Membro Posterior
Músculo Esquelético/patologia
Ratos
Ratos Sprague-Dawley
Pele/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE
[do] DOI:10.3969/j.issn.1004-5619.2017.01.001


  3 / 20758 MEDLINE  
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[PMID]:29330588
[Au] Autor:Provini P; Abourachid A
[Ad] Endereço:Department of Adaptations du Vivant, National Museum of Natural History, UMR 7179, AVIV, 57 rue Cuvier, case postale 55, Paris, 75231, France. pauline.provini@mnhn.fr.
[Ti] Título:Whole-body 3D kinematics of bird take-off: key role of the legs to propel the trunk.
[So] Source:Naturwissenschaften;105(1-2):12, 2018 Jan 06.
[Is] ISSN:1432-1904
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Previous studies showed that birds primarily use their hindlimbs to propel themselves into the air in order to take-off. Yet, it remains unclear how the different parts of their musculoskeletal system move to produce the necessary acceleration. To quantify the relative motions of the bones during the terrestrial phase of take-off, we used biplanar fluoroscopy in two species of birds, diamond dove (Geopelia cuneata) and zebra finch (Taeniopygia guttata). We obtained a detailed 3D kinematics analysis of the head, the trunk and the three long bones of the left leg. We found that the entire body assisted the production of the needed forces to take-off, during two distinct but complementary phases. The first one, a relatively slow preparatory phase, started with a movement of the head and an alignment of the different groups of bones with the future take-off direction. It was associated with a pitch down of the trunk and a flexion of the ankle, of the hip and, to a lesser extent, of the knee. This crouching movement could contribute to the loading of the leg muscles and store elastic energy that could be released in the propulsive phase of take-off, during the extension of the leg joints. Combined with the fact that the head, together with the trunk, produced a forward momentum, the entire body assisted the production of the needed forces to take-off. The second phase was faster with mostly horizontal forward and vertical upward translation motions, synchronous to an extension of the entire lower articulated musculoskeletal system. It led to the propulsion of the bird in the air with a fundamental role of the hip and ankle joints to move the trunk upward and forward. Take-off kinematics were similar in both studied species, with a more pronounced crouching movement in diamond dove, which can be related to a large body mass compared to zebra finch.
[Mh] Termos MeSH primário: Columbidae/fisiologia
Tentilhões/fisiologia
Voo Animal/fisiologia
Membro Posterior/fisiologia
[Mh] Termos MeSH secundário: Aceleração
Animais
Fenômenos Biomecânicos
Osso e Ossos/fisiologia
Columbidae/anatomia & histologia
Tentilhões/anatomia & histologia
Fluoroscopia
Membro Posterior/anatomia & histologia
Músculo Esquelético/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180114
[St] Status:MEDLINE
[do] DOI:10.1007/s00114-017-1535-8


  4 / 20758 MEDLINE  
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[PMID]:29329564
[Au] Autor:Owolabi OO; James DB; Sani I; Andongma BT; Fasanya OO; Kure B
[Ad] Endereço:Bioresources Development Centre, National Biotechnology Development Agency, Ya'adua way, Lugbe, Airport Road, Abuja, FCT, Nigeria. drealyinks@yahoo.com.
[Ti] Título:Phytochemical analysis, antioxidant and anti-inflammatory potential of FERETIA APODANTHERA root bark extracts.
[So] Source:BMC Complement Altern Med;18(1):12, 2018 Jan 12.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Inflammation has been implicated in many disorders, including cancer and available therapies elicit adverse effects. Plants of the family Rubiaceae have shown potency against inflammation. The anti-inflammatory and anti-oxidant potential of Feretia apodanthera was investigated in this study to evaluate its effectiveness. METHODS: The phytochemical, antioxidant and anti-inflammatory potential of root bark (n-Hexane, diethyl ether, ethanol and aqueous) extracts of Feretia apodanthera was investigated in this study. The extracts were subjected to various chemical tests for phytochemical constituents; their antioxidant activity was determined using in-vitro DPPH radical scavenging activity assay and their anti-inflammatory activity was determined using carrageenan induced paw oedema model. FTIR and GCMS analysis was done to determine the compounds present. RESULTS: Phytochemical screening of extracts revealed the presence of unsaturated steroids, triterpenes, cardiac glycosides, tannins, saponin and alkaloids. Vitamin C had a median inhibitory concentration (IC ) of 0.038 mg/ml which was lower than IC of all the extracts. Of all the extracts, ethanol extract had the lowest IC (0.044 mg/ml) which is comparable to vitamin C. Anti-inflammatory studies showed that the inflammation inhibition potential of 400 mg/kg body weight of all the extracts was significantly lower (p < 0.05) than the standard ketoprofen (50 mg/kg) at the first three hours but significantly higher (p < 0.05) at the fourth hour. At the fifth hour, the inflammation inhibition potential of diethyl ether, ethanol and aqueous extracts were significantly higher (p < 0.05) than that of the standard. FTIR analysis showed the presence of ketones, amines, alkenes and carboxylic groups. GCMS analysis revealed compounds that are potential anti-inflammatory agents. CONCLUSION: This study revealed that extracts of Feretia apodanthera possess anti-inflammatory effects against right hind paw oedema of albino rats and can act as an effective antioxidant.
[Mh] Termos MeSH primário: Anti-Inflamatórios/química
Antioxidantes/química
Extratos Vegetais/química
Raízes de Plantas/química
Rubiaceae/química
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios/farmacologia
Antioxidantes/farmacologia
Edema/patologia
Feminino
Membro Posterior/efeitos dos fármacos
Inflamação/patologia
Masculino
Casca de Planta/química
Extratos Vegetais/farmacologia
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Antioxidants); 0 (Plant Extracts)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180114
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-2070-z


  5 / 20758 MEDLINE  
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[PMID]:28461107
[Au] Autor:Gertel S; Mahagna H; Karmon G; Watad A; Amital H
[Ad] Endereço:Zabludowicz Center For Autoimmune Diseases, Sheba Medical Center, Tel-Hashomer 5262100, Israel; Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv 6997801, Israel. Electronic address: smadar.gertel@sheba.health.gov.il.
[Ti] Título:Tofacitinib attenuates arthritis manifestations and reduces the pathogenic CD4 T cells in adjuvant arthritis rats.
[So] Source:Clin Immunol;184:77-81, 2017 11.
[Is] ISSN:1521-7035
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Rheumatoid arthritis (RA) is an autoimmune disease characterized by pronounced inflammation and leukocyte infiltration in affected joints. Tofacitinib is new agent, a selective inhibitor of Janus kinase (JAK) signaling pathways mediated by JAK1 and JAK3 and inhibits the key transcription factors STAT1 and STAT3. We investigated the action mechanisms of tofacitinib in rats with adjuvant-induced-arthritis (AIA). AIA-rats were treated orally with tofacitinib or with methotrexate. Arthritis severity and serum C-reactive protein (CRP) levels were evaluated, splenic cells were examined by flow cytometry and cytokines were analyzed by real-time PCR. Tofacitinib markedly reduced the clinical status of treated rats in comparison to control group. Reduced joints inflammation and down-regulated serum CRP levels reflected the clinical manifestations of the treated rats. Tofacitinib down-regulated significantly the frequency of CD4 IFN-γ T cells and reduced IL-1ß mRNA expression levels in the spleen of the treated rats. These results show that tofacitinib attenuated arthritis severity, modified splenic populations and cytokine imbalance.
[Mh] Termos MeSH primário: Artrite Experimental/imunologia
Artrite Reumatoide/imunologia
Linfócitos T CD4-Positivos/efeitos dos fármacos
Articulações do Pé/efeitos dos fármacos
Piperidinas/farmacologia
Inibidores de Proteínas Quinases/farmacologia
Pirimidinas/farmacologia
Pirróis/farmacologia
[Mh] Termos MeSH secundário: Animais
Antirreumáticos/farmacologia
Artrite Experimental/fisiopatologia
Artrite Reumatoide/fisiopatologia
Proteína C-Reativa/efeitos dos fármacos
Proteína C-Reativa/imunologia
Linfócitos T CD4-Positivos/imunologia

Articulações do Pé/patologia
Membro Anterior
Membro Posterior
Interferon gama/imunologia
Interleucina-1beta/efeitos dos fármacos
Interleucina-1beta/genética
Metotrexato/farmacologia
RNA Mensageiro/efeitos dos fármacos
RNA Mensageiro/metabolismo
Ratos
Reação em Cadeia da Polimerase em Tempo Real
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Baço/citologia
Baço/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antirheumatic Agents); 0 (IL1B protein, rat); 0 (Interleukin-1beta); 0 (Piperidines); 0 (Protein Kinase Inhibitors); 0 (Pyrimidines); 0 (Pyrroles); 0 (RNA, Messenger); 82115-62-6 (Interferon-gamma); 87LA6FU830 (tofacitinib); 9007-41-4 (C-Reactive Protein); YL5FZ2Y5U1 (Methotrexate)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE


  6 / 20758 MEDLINE  
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[PMID]:29235764
[Au] Autor:Marchenko MM; Ketsa OV; Shmarakov IO; Abutnaritsa KH
[Ti] Título:Monooxygenase system in Guerin's carcinoma of rats under conditions of ω-3 polyunsaturated fatty acids administration.
[So] Source:Ukr Biochem J;88(4):48-56, 2016 Jul-Aug.
[Is] ISSN:2409-4943
[Cp] País de publicação:Ukraine
[La] Idioma:eng
[Ab] Resumo:The aim of the study was to determine the variations of function in components of monooxygenase system (MOS) of rat Guerin's carcinoma under ω-3 polyunsaturated fatty acids (PUFAs) administration. The activity of Guerin's carcinoma microsomal NADH-cytochrome b5 reductase, the content and the rate of cytochrome b5 oxidation-reduction, the content and the rate of cytochrome Р450 oxidation-reduction have been investigated in rats with tumor under conditions of ω-3 PUFAs administration. ω-3 PUFAs supplementation before and after transplantation of Guerin's carcinoma resulted in the increase of NADH-cytochrome b5 reductase activity and decrease of cytochrome b5 level in the Guerin's carcinoma microsomal fraction in the logarithmic phases of carcinogenesis as compared to the tumor-bearing rats. Increased activity of NADH-cytochrome b5 reductase facilitates higher electron flow in redox-chain of MOS. Under decreased cytochrome b5 levels the electrons are transferred to oxygen, which leads to heightened generation of superoxide (O2•-) in comparison to control. It was shown, that the decrease of cytochrome P450 level in the Guerin's carcinoma microsomal fraction in the logarithmic phases of oncogenesis under ω-3 PUFAs administration may be associated with its transition into an inactive form ­ cytochrome P420. This decrease in cytochrome P450 coincides with increased generation of superoxide by MOS oxygenase chain.
[Mh] Termos MeSH primário: Carcinoma/tratamento farmacológico
Elétrons
Ácidos Graxos Ômega-3/farmacologia
Expressão Gênica/efeitos dos fármacos
Microssomos/efeitos dos fármacos
Substâncias Protetoras/farmacologia
[Mh] Termos MeSH secundário: Animais
Carcinoma/enzimologia
Carcinoma/patologia
Sistema Enzimático do Citocromo P-450/genética
Sistema Enzimático do Citocromo P-450/metabolismo
Citocromo-B(5) Redutase/genética
Citocromo-B(5) Redutase/metabolismo
Citocromos/genética
Citocromos/metabolismo
Citocromos b5/genética
Citocromos b5/metabolismo
Transporte de Elétrons/efeitos dos fármacos
Feminino
Membro Posterior
Injeções Subcutâneas
Microssomos/enzimologia
Transplante de Neoplasias
Oxirredução/efeitos dos fármacos
Estresse Oxidativo/efeitos dos fármacos
Ratos
Superóxidos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytochromes); 0 (Fatty Acids, Omega-3); 0 (Protective Agents); 11062-77-4 (Superoxides); 9035-39-6 (Cytochromes b5); 9035-49-8 (cytochrome P420); 9035-51-2 (Cytochrome P-450 Enzyme System); EC 1.6.2.2 (Cytochrome-B(5) Reductase)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.15407/ubj88.04.048


  7 / 20758 MEDLINE  
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[PMID]:29235761
[Au] Autor:Sydor RI; Khranovska NM; Skachkova OV; Skivka LM
[Ti] Título:The effect of perioperative analgesic drugs omnopon and dexketoprofen on the functional activity of immune cells in murine model of tumor surgery.
[So] Source:Ukr Biochem J;88(4):40-7, 2016 Jul-Aug.
[Is] ISSN:2409-4943
[Cp] País de publicação:Ukraine
[La] Idioma:eng
[Ab] Resumo:We aimed to investigate the effect of perioperative analgesia with nonselective cyclooxygenase-2 inhibitor dexketoprofen and opioid drug omnopon on the functional activity of immune cells in tumor excision murine model. Lewis lung carcinoma cells were transplanted into hind paw of C57/black mice. On the 23th day tumor was removed. Analgesic drugs were injected 30 min before and once a day for 3 days after the surgery. Biological material was obtained a day before, 1 day and 3 days after the tumor removal. IFN-γ, IL-4, IL-10 and TGF-ß mRNA levels in splenic cells were assessed by quantitative real-time RT-PCR. Cytotoxic activity of splenocytes was estimated by flow cytometry. We found that in splenocytes of mice received opioid analgesia IL-10 mRNA level was increased 2.3 times on day one after the surgery compared to preoperative level (P < 0.05), while in dexketoprofen group this parameter did not change. IFN-γ gene expression level on day 3 after tumor removal was 40% higher in splenocytes of dexketoprofen treated mice as compared with omnopon treated animals (P < 0.05). Cytotoxic activity of splenocytes on day 3 postsurgery was (62.2 ± 2.4)% in dexketoprofen against (50.2 ± 3.3)% in omnopon group. In conclusion, perioperative analgesia with cyclooxygenase inhibitor dexketoprofen in contrast to opioid analgesia with omnopon preserves higher functional activity of murine immune cells in the experimental model of tumor surgery.
[Mh] Termos MeSH primário: Analgésicos/farmacologia
Carcinoma Pulmonar de Lewis/imunologia
Citotoxicidade Imunológica/efeitos dos fármacos
Cetoprofeno/farmacologia
Linfócitos/efeitos dos fármacos
Ópio/farmacologia
Dor Processual/prevenção & controle
[Mh] Termos MeSH secundário: Animais
Carcinoma Pulmonar de Lewis/genética
Carcinoma Pulmonar de Lewis/patologia
Carcinoma Pulmonar de Lewis/cirurgia
Expressão Gênica
Membro Posterior
Interferon gama/genética
Interferon gama/imunologia
Interleucina-10/genética
Interleucina-10/imunologia
Interleucina-4/genética
Interleucina-4/imunologia
Cetoprofeno/análogos & derivados
Linfócitos/citologia
Linfócitos/imunologia
Camundongos
Camundongos Endogâmicos C57BL
Transplante de Neoplasias
Dor Processual/imunologia
Dor Processual/fisiopatologia
Período Perioperatório
RNA Mensageiro/genética
RNA Mensageiro/imunologia
Baço/citologia
Baço/efeitos dos fármacos
Baço/imunologia
Fator de Crescimento Transformador beta/genética
Fator de Crescimento Transformador beta/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Analgesics); 0 (IL10 protein, mouse); 0 (RNA, Messenger); 0 (Transforming Growth Factor beta); 130068-27-8 (Interleukin-10); 207137-56-2 (Interleukin-4); 8008-60-4 (Opium); 82115-62-6 (Interferon-gamma); 90Y4QC304K (Ketoprofen)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.15407/ubj88.04.040


  8 / 20758 MEDLINE  
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[PMID]:27773721
[Au] Autor:Tajerian M; Hung V; Khan H; Lahey LJ; Sun Y; Birklein F; Krämer HH; Robinson WH; Kingery WS; Clark JD
[Ad] Endereço:Veterans Affairs Palo Alto Health Care System Palo Alto, CA, USA; Department of Anesthesiology, Stanford University School of Medicine, Stanford, CA, USA; Palo Alto Veterans Institute for Research, Palo Alto, CA, USA. Electronic address: maral@stanford.edu.
[Ti] Título:Identification of KRT16 as a target of an autoantibody response in complex regional pain syndrome.
[So] Source:Exp Neurol;287(Pt 1):14-20, 2017 Jan.
[Is] ISSN:1090-2430
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Using a mouse model of complex regional pain syndrome (CRPS), our goal was to identify autoantigens in the skin of the affected limb. METHODS: A CRPS-like state was induced using the tibia fracture/cast immobilization model. Three weeks after fracture, hindpaw skin was homogenized, run on 2-d gels, and probed by sera from fracture and control mice. Spots of interest were analyzed by liquid chromatography-mass spectroscopy (LC-MS) and the list of targets validated by examining their abundance and subcellular localization. In order to measure the autoantigenicity of selected protein targets, we quantified the binding of IgM in control and fracture mice sera, as well as in control and CRPS human sera, to the recombinant protein. RESULTS: We show unique binding between fracture skin extracts and fracture sera, suggesting the presence of auto-antigens. LC-MS analysis provided us a list of potential targets, some of which were upregulated after fracture (KRT16, eEF1a1, and PRPH), while others showed subcellular-redistribution and increased membrane localization (ANXA2 and ENO3). No changes in protein citrullination or carbamylation were observed. In addition to increased abundance, KRT16 demonstrated autoantigenicity, since sera from both fracture mice and CRPS patients showed increased autoantibody binding to recombinant kRT16 protein. CONCLUSIONS: Pursuing autoimmune contributions to CRPS provides a novel approach to understanding the condition and may allow the development of mechanism-based therapies. The identification of autoantibodies against KRT16 as a biomarker in mice and in humans is a critical step towards these goals, and towards redefining CRPS as having an autoimmune etiology.
[Mh] Termos MeSH primário: Autoantígenos/metabolismo
Síndromes da Dor Regional Complexa/sangue
Síndromes da Dor Regional Complexa/patologia
Queratina-6/imunologia
Queratina-6/metabolismo
Pele/metabolismo
Pele/ultraestrutura
Regulação para Cima/fisiologia
[Mh] Termos MeSH secundário: Adulto
Animais
Anexina A2/metabolismo
Autoantígenos/genética
Modelos Animais de Doenças
Membro Posterior/inervação
Seres Humanos
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Meia-Idade
Fator 1 de Elongação de Peptídeos/metabolismo
Periferinas/metabolismo
Fosfopiruvato Hidratase/metabolismo
Frações Subcelulares/metabolismo
Fraturas da Tíbia/sangue
Fraturas da Tíbia/patologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ANXA2 protein, human); 0 (Annexin A2); 0 (Autoantigens); 0 (EEF1A1 protein, human); 0 (KRT6A protein, human); 0 (Keratin-6); 0 (PRPH protein, human); 0 (Peptide Elongation Factor 1); 0 (Peripherins); EC 4.2.1.11 (Phosphopyruvate Hydratase)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:180101
[Lr] Data última revisão:
180101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161028
[St] Status:MEDLINE


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[PMID]:28461333
[Au] Autor:Taghiyar L; Hesaraki M; Sayahpour FA; Satarian L; Hosseini S; Aghdami N; Baghaban Eslaminejad M
[Ad] Endereço:From the Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, Academic Center for Education, Culture and Research (ACECR), Tehran 1665659911, Iran and.
[Ti] Título:Msh homeobox 1 ( )- and -overexpressing bone marrow-derived mesenchymal stem cells resemble blastema cells and enhance regeneration in mice.
[So] Source:J Biol Chem;292(25):10520-10533, 2017 06 23.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Amputation of the proximal region in mammals is not followed by regeneration because blastema cells (BCs) and expression of regenerative genes, such as Msh homeobox ( ) genes, are absent in this animal group. The lack of BCs and positional information in other cells is therefore the main obstacle to therapeutic approaches for limb regeneration. Hence, this study aimed to create blastema-like cells (BlCs) by overexpressing and genes in mouse bone marrow-derived mesenchymal stem cells (mBMSCs) to regenerate a proximally amputated digit tip. We transduced mBMSCs with and genes and compared osteogenic activity and expression levels of several -regulated genes ( , , and keratin 14 ( )) in BlC groups, including MSX1, MSX2, and MSX1/2 (in a 1:1 ratio) with those in mBMSCs and BCs and following injection into the amputation site. We found that gene overexpression increased expression of specific blastemal markers and enhanced the proliferation rate and osteogenesis of BlCs compared with mBMSCs and BCs via activation of and Histological analyses indicated full regrowth of digit tips in the -overexpressing groups, particularly in MSX1/2, through endochondral ossification 6 weeks post-injection. In contrast, mBMSCs and BCs formed abnormal bone and nail. Full digit tip was regenerated only in the MSX1/2 group and was related to boosted , and gene expression and to limb-patterning properties resulting from and overexpression. We propose that -transduced cells that can regenerate epithelial and mesenchymal tissues may potentially be utilized in limb regeneration.
[Mh] Termos MeSH primário: Células da Medula Óssea/metabolismo
Membro Posterior/fisiologia
Proteínas de Homeodomínio/biossíntese
Fator de Transcrição MSX1/biossíntese
Transplante de Células-Tronco Mesenquimais
Células Mesenquimais Estromais/metabolismo
Osteogênese
Regeneração
[Mh] Termos MeSH secundário: Aloenxertos
Animais
Proteína Morfogenética Óssea 4/biossíntese
Proteína Morfogenética Óssea 4/genética
Proliferação Celular/genética
Fator 8 de Crescimento de Fibroblasto/biossíntese
Fator 8 de Crescimento de Fibroblasto/genética
Proteínas de Homeodomínio/genética
Queratina-14/biossíntese
Queratina-14/genética
Fator de Transcrição MSX1/genética
Camundongos
Transdução Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bmp4 protein, mouse); 0 (Bone Morphogenetic Protein 4); 0 (Fgf8 protein, mouse); 0 (Homeodomain Proteins); 0 (Keratin-14); 0 (Krt1-14 protein, mouse); 0 (MSX1 Transcription Factor); 0 (MSX2 protein); 0 (Msx1 protein, mouse); 148997-75-5 (Fibroblast Growth Factor 8)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171229
[Lr] Data última revisão:
171229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M116.774265


  10 / 20758 MEDLINE  
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[PMID]:29040289
[Au] Autor:Doi A; Miyamoto K; Nakano YS; Sakasaki J; Kasae S; Nishimura K; Shin MC; Yoshimura M
[Ad] Endereço:Department of Rehabilitation, Kumamoto Health Science University, Kumamoto, Kumamoto, Japan.
[Ti] Título:Sole vibration improves locomotion through the recovery of joint movements in a mouse cast model.
[So] Source:PLoS One;12(10):e0186189, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We investigated the effects of a vibratory stimulus on the plantar surface of the hind limb for motor, sensory, and locomotive function using a mouse cast model. The right knee joint of C57BL/6 male mice (7 weeks, 20 g, n = 31) was flexed with aluminum splint and tape for 6 weeks. These mice were randomly divided into 2 groups (control group, n = 11 and vibration group, n = 12). The mice in the vibration group received vibration on the sole of the ankle for 15 minutes per day, 5 days per week. After the knee joint cast was removed, we measured the range of motion (ROM) of both knee and ankle joints and the sensory threshold of the sole. Further, both walking and swimming movements were analyzed with a digital video. The sole vibration did not affect the passive ROM of the knee joint and sensory threshold after cast removal. However, it increased the ankle dorsiflexion range and improved free walking, swimming, and active movement of the knee joint. In conclusion, we show that the vibration recovered both walking and swimming movements, which resulted from improvements in both the passive ankle dorsiflexion and active knee movement.
[Mh] Termos MeSH primário: Articulação do Joelho/fisiologia
Locomoção/fisiologia
Vibração/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Articulação do Tornozelo/fisiologia
Articulação do Tornozelo/fisiopatologia
Fenômenos Biomecânicos
Membro Posterior/fisiologia
Membro Posterior/fisiopatologia
Articulação do Joelho/fisiopatologia
Camundongos
Amplitude de Movimento Articular/fisiologia
Caminhada
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171018
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186189



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