Base de dados : MEDLINE
Pesquisa : A13.564 [Categoria DeCS]
Referências encontradas : 385 [refinar]
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[PMID]:28468922
[Au] Autor:Herzog H; Klein B; Ziegler A
[Ad] Endereço:Institut für Zoologie, Rheinische Friedrich-Wilhelms-Universität Bonn, Poppelsdorfer Schloss, 53115 Bonn, Germany hendrik.herzog@uni-bonn.de.
[Ti] Título:Form and function of the teleost lateral line revealed using three-dimensional imaging and computational fluid dynamics.
[So] Source:J R Soc Interface;14(130), 2017 May.
[Is] ISSN:1742-5662
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Fishes sense weak water motion using the lateral line. Among the thousands of described fish species, this organ may differ in size, shape and distribution of individual mechanoreceptors or lateral line canals. The reasons for this diversity remain unclear, but are very likely related to habitat preferences. To better understand the performance of the organ in natural hydrodynamic surroundings, various three-dimensional imaging datasets of the cephalic lateral line were gathered using as representative freshwater teleost. These data are employed to simulate hydrodynamic phenomena around the head and within lateral line canals. The results show that changes in canal dimensions alter the absolute stimulation amplitudes, but have little effect on the relation between bulk water flow and higher frequency signals. By contrast, depressions in the skin known as epidermal pits reduce bulk flow stimulation and increase the ratio between higher-frequency signals and the background flow stimulus.
[Mh] Termos MeSH primário: Peixes
Imagem Tridimensional
Sistema da Linha Lateral
Modelos Biológicos
Reologia
[Mh] Termos MeSH secundário: Animais
Peixes/anatomia & histologia
Peixes/fisiologia
Sistema da Linha Lateral/anatomia & histologia
Sistema da Linha Lateral/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE


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[PMID]:28923486
[Au] Autor:Nikaido M; Acedo JN; Hatta K; Piotrowski T
[Ad] Endereço:Stowers Institute for Medical Research, Kansas City, MO 64110, USA; Graduate School of Life Sciences, University of Hyogo, Hyogo Pref. 678-1297, Japan.
[Ti] Título:Retinoic acid is required and Fgf, Wnt, and Bmp signaling inhibit posterior lateral line placode induction in zebrafish.
[So] Source:Dev Biol;431(2):215-225, 2017 11 15.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The lateral line system is a mechanosensory systems present in aquatic animals. The anterior and posterior lateral lines develop from anterior and posterior lateral line placodes (aLLp and pLLp), respectively. Although signaling molecules required for the induction of other cranial placodes have been well studied, the molecular mechanisms underlying formation of the lateral line placodes are unknown. In this study we tested the requirement of multiple signaling pathways, such as Wnt, Bmp Fgf, and Retinoic Acid for aLLp and pLLp induction. We determined that aLLp specification requires Fgf signaling, whilst pLLp specification requires retinoic acid which inhibits Fgf signaling. pLLp induction is also independent of Wnt and Bmp activities, even though these pathways limit the boundaries of the pLLp. This is the first report that the aLLp and pLLp depend on different inductive mechanisms and that pLLp induction requires the inhibition of Fgf, Wnt and Bmp signaling.
[Mh] Termos MeSH primário: Proteínas Morfogenéticas Ósseas/metabolismo
Fatores de Crescimento de Fibroblastos/metabolismo
Sistema da Linha Lateral/embriologia
Transdução de Sinais
Tretinoína/farmacologia
Proteínas Wnt/metabolismo
Peixe-Zebra/embriologia
Peixe-Zebra/metabolismo
[Mh] Termos MeSH secundário: Animais
Benzaldeídos/farmacologia
Padronização Corporal/efeitos dos fármacos
Padronização Corporal/genética
Gastrulação/efeitos dos fármacos
Sistema da Linha Lateral/efeitos dos fármacos
Sistema da Linha Lateral/metabolismo
Transdução de Sinais/efeitos dos fármacos
Proteínas de Peixe-Zebra/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Benzaldehydes); 0 (Bone Morphogenetic Proteins); 0 (Wnt Proteins); 0 (Zebrafish Proteins); 5688UTC01R (Tretinoin); 62031-54-3 (Fibroblast Growth Factors)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171111
[Lr] Data última revisão:
171111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170920
[St] Status:MEDLINE


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[PMID]:28818669
[Au] Autor:Modrell MS; Tidswell ORA; Baker CVH
[Ad] Endereço:Department of Physiology, Development and Neuroscience, University of Cambridge, Anatomy Building, Downing Street, Cambridge CB2 3DY, UK.
[Ti] Título:Notch and Fgf signaling during electrosensory versus mechanosensory lateral line organ development in a non-teleost ray-finned fish.
[So] Source:Dev Biol;431(1):48-58, 2017 11 01.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The lateral line system is a useful model for studying the embryonic and evolutionary diversification of different organs and cell types. In jawed vertebrates, this ancestrally comprises lines of mechanosensory neuromasts over the head and trunk, flanked on the head by fields of electrosensory ampullary organs, all innervated by lateral line neurons in cranial lateral line ganglia. Both types of sense organs, and their afferent neurons, develop from cranial lateral line placodes. Current research primarily focuses on the posterior lateral line primordium in zebrafish, which migrates as a cell collective along the trunk; epithelial rosettes form in the trailing zone and are deposited as a line of neuromasts, within which hair cells and supporting cells differentiate. However, in at least some other teleosts (e.g. catfishes) and all non-teleosts, lines of cranial neuromasts are formed by placodes that elongate to form a sensory ridge, which subsequently fragments, with neuromasts differentiating in a line along the crest of the ridge. Furthermore, in many non-teleost species, electrosensory ampullary organs develop from the flanks of the sensory ridge. It is unknown to what extent the molecular mechanisms underlying neuromast formation from the zebrafish migrating posterior lateral line primordium are conserved with the as-yet unexplored molecular mechanisms underlying neuromast and ampullary organ formation from elongating lateral line placodes. Here, we report experiments in an electroreceptive non-teleost ray-finned fish, the Mississippi paddlefish Polyodon spathula, that suggest a conserved role for Notch signaling in regulating lateral line organ receptor cell number, but potentially divergent roles for the fibroblast growth factor signaling pathway, both between neuromasts and ampullary organs, and between paddlefish and zebrafish.
[Mh] Termos MeSH primário: Fatores de Crescimento de Fibroblastos/metabolismo
Proteínas de Peixes/metabolismo
Peixes/crescimento & desenvolvimento
Peixes/metabolismo
Sistema da Linha Lateral/crescimento & desenvolvimento
Sistema da Linha Lateral/metabolismo
Mecanorreceptores/metabolismo
Receptores Notch/metabolismo
[Mh] Termos MeSH secundário: Animais
Fatores de Crescimento de Fibroblastos/genética
Proteínas de Peixes/genética
Peixes/genética
Regulação da Expressão Gênica no Desenvolvimento
Hibridização In Situ
Receptores Notch/genética
Órgãos dos Sentidos/crescimento & desenvolvimento
Órgãos dos Sentidos/inervação
Órgãos dos Sentidos/metabolismo
Células Receptoras Sensoriais/metabolismo
Transdução de Sinais
Peixe-Zebra/genética
Peixe-Zebra/crescimento & desenvolvimento
Peixe-Zebra/metabolismo
Proteínas de Peixe-Zebra/genética
Proteínas de Peixe-Zebra/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fish Proteins); 0 (Receptors, Notch); 0 (Zebrafish Proteins); 62031-54-3 (Fibroblast Growth Factors)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171111
[Lr] Data última revisão:
171111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170819
[St] Status:MEDLINE


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[PMID]:28707626
[Au] Autor:Boulogne LH; Wolf BJ; Wiering MA; van Netten SM
[Ad] Endereço:Institute of Artificial Intelligence and Cognitive Engineering, University of Groningen, 9700 AK Groningen, Netherlands.
[Ti] Título:Performance of neural networks for localizing moving objects with an artificial lateral line.
[So] Source:Bioinspir Biomim;12(5):056009, 2017 Sep 26.
[Is] ISSN:1748-3190
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Fish are able to sense water flow velocities relative to their body with their mechanoreceptive lateral line organ. This organ consists of an array of flow detectors distributed along the fish body. Using the excitation of these individual detectors, fish can determine the location of nearby moving objects. Inspired by this sensory modality, it is shown here how neural networks can be used to extract an object's location from simulated excitation patterns, as can be measured along arrays of stationary artificial flow velocity sensors. The applicability, performance and robustness with respect to input noise of different neural network architectures are compared. When trained and tested under high signal to noise conditions (46 dB), the Extreme Learning Machine architecture performs best with a mean Euclidean error of 0.4% of the maximum depth of the field D, which is taken half the length of the sensor array. Under lower signal to noise conditions Echo State Networks, having recurrent connections, enhance the performance while the Multilayer Perceptron is shown to be the most noise robust architecture. Neural network performance decreased when the source moves close to the sensor array or to the sides of the array. For all considered architectures, increasing the number of detectors per array increased localization performance and robustness.
[Mh] Termos MeSH primário: Materiais Biomiméticos/normas
Sistema da Linha Lateral
Redes Neurais (Computação)
[Mh] Termos MeSH secundário: Animais
Peixes/fisiologia
Sistema da Linha Lateral/fisiologia
Mecanorreceptores/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170715
[St] Status:MEDLINE
[do] DOI:10.1088/1748-3190/aa7fcb


  5 / 385 MEDLINE  
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[PMID]:28546313
[Au] Autor:Sheets L; He XJ; Olt J; Schreck M; Petralia RS; Wang YX; Zhang Q; Beirl A; Nicolson T; Marcotti W; Trapani JG; Kindt KS
[Ad] Endereço:Department of Otolaryngology, Harvard Medical School, Boston, Massachusetts 02115.
[Ti] Título:Enlargement of Ribbons in Zebrafish Hair Cells Increases Calcium Currents But Disrupts Afferent Spontaneous Activity and Timing of Stimulus Onset.
[So] Source:J Neurosci;37(26):6299-6313, 2017 Jun 28.
[Is] ISSN:1529-2401
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In sensory hair cells of auditory and vestibular organs, the ribbon synapse is required for the precise encoding of a wide range of complex stimuli. Hair cells have a unique presynaptic structure, the synaptic ribbon, which organizes both synaptic vesicles and calcium channels at the active zone. Previous work has shown that hair-cell ribbon size is correlated with differences in postsynaptic activity. However, additional variability in postsynapse size presents a challenge to determining the specific role of ribbon size in sensory encoding. To selectively assess the impact of ribbon size on synapse function, we examined hair cells in transgenic zebrafish that have enlarged ribbons, without postsynaptic alterations. Morphologically, we found that enlarged ribbons had more associated vesicles and reduced presynaptic calcium-channel clustering. Functionally, hair cells with enlarged ribbons had larger global and ribbon-localized calcium currents. Afferent neuron recordings revealed that hair cells with enlarged ribbons resulted in reduced spontaneous spike rates. Additionally, despite larger presynaptic calcium signals, we observed fewer evoked spikes with longer latencies from stimulus onset. Together, our work indicates that hair-cell ribbon size influences the spontaneous spiking and the precise encoding of stimulus onset in afferent neurons. Numerous studies support that hair-cell ribbon size corresponds with functional sensitivity differences in afferent neurons and, in the case of inner hair cells of the cochlea, vulnerability to damage from noise trauma. Yet it is unclear whether ribbon size directly influences sensory encoding. Our study reveals that ribbon enlargement results in increased ribbon-localized calcium signals, yet reduces afferent spontaneous activity and disrupts the timing of stimulus onset, a distinct aspect of auditory and vestibular encoding. These observations suggest that varying ribbon size alone can influence sensory encoding, and give further insight into how hair cells transduce signals that cover a wide dynamic range of stimuli.
[Mh] Termos MeSH primário: Potenciais de Ação/fisiologia
Sinalização do Cálcio/fisiologia
Mecanorreceptores/citologia
Mecanorreceptores/fisiologia
Tempo de Reação/fisiologia
Peixe-Zebra/fisiologia
[Mh] Termos MeSH secundário: Animais
Animais Geneticamente Modificados
Tamanho Celular
Sistema da Linha Lateral/citologia
Sistema da Linha Lateral/fisiologia
Inibição Neural/fisiologia
Peixe-Zebra/anatomia & histologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170818
[Lr] Data última revisão:
170818
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170527
[St] Status:MEDLINE
[do] DOI:10.1523/JNEUROSCI.2878-16.2017


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[PMID]:28239851
[Au] Autor:Bradney DR; Davidson A; Evans SP; Wueringer BE; Morgan DL; Clausen PD
[Ad] Endereço:School of Engineering, The University of Newcastle, Callaghan, NSW, 2308, Australia.
[Ti] Título:Sawfishes stealth revealed using computational fluid dynamics.
[So] Source:J Fish Biol;90(4):1584-1596, 2017 Apr.
[Is] ISSN:1095-8649
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Detailed computational fluid dynamics simulations for the rostrum of three species of sawfish (Pristidae) revealed that negligible turbulent flow is generated from all rostra during lateral swipe prey manipulation and swimming. These results suggest that sawfishes are effective stealth hunters that may not be detected by their teleost prey's lateral line sensory system during pursuits. Moreover, during lateral swipes, the rostra were found to induce little velocity into the surrounding fluid. Consistent with previous data of sawfish feeding behaviour, these data indicate that the rostrum is therefore unlikely to be used to stir up the bottom to uncover benthic prey. Whilst swimming with the rostrum inclined at a small angle to the horizontal, the coefficient of drag of the rostrum is relatively low and the coefficient of lift is zero.
[Mh] Termos MeSH primário: Comportamento Alimentar
Hidrodinâmica
Raias/fisiologia
Natação/fisiologia
[Mh] Termos MeSH secundário: Animais
Sistema da Linha Lateral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170228
[St] Status:MEDLINE
[do] DOI:10.1111/jfb.13255


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[PMID]:28100806
[Au] Autor:Jaggard J; Robinson BG; Stahl BA; Oh I; Masek P; Yoshizawa M; Keene AC
[Ad] Endereço:Department of Biological Sciences, Florida Atlantic University, Jupiter, FL 33458, USA.
[Ti] Título:The lateral line confers evolutionarily derived sleep loss in the Mexican cavefish.
[So] Source:J Exp Biol;220(Pt 2):284-293, 2017 Jan 15.
[Is] ISSN:1477-9145
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Sleep is an essential behavior exhibited by nearly all animals, and disruption of this process is associated with an array of physiological and behavioral deficits. Sleep is defined by changes in sensory gating that reduce sensory input to the brain, but little is known about the neural basis for interactions between sleep and sensory processing. Blind Mexican cavefish comprise an extant surface dwelling form and 29 cave morphs that have independently evolved increased numbers of mechanoreceptive lateral line neuromasts and convergent evolution of sleep loss. Ablation of the lateral line enhanced sleep in the Pachón cavefish population, suggesting that heightened sensory input underlies evolutionarily derived sleep loss. Targeted lateral line ablation and behavioral analysis localized the wake-promoting neuromasts in Pachón cavefish to superficial neuromasts of the trunk and cranial regions. Strikingly, lateral line ablation did not affect sleep in four other cavefish populations, suggesting that distinct neural mechanisms regulate the evolution of sleep loss in independently derived cavefish populations. Cavefish are subject to seasonal changes in food availability, raising the possibility that sensory modulation of sleep is influenced by metabolic state. We found that starvation promotes sleep in Pachón cavefish, and is not enhanced by lateral line ablation, suggesting that functional interactions occur between sensory and metabolic regulation of sleep. Taken together, these findings support a model where sensory processing contributes to evolutionarily derived changes in sleep that are modulated in accordance with food availability.
[Mh] Termos MeSH primário: Characidae/fisiologia
Sistema da Linha Lateral/fisiologia
Sono
[Mh] Termos MeSH secundário: Animais
Evolução Biológica
Cavernas
México
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170120
[St] Status:MEDLINE
[do] DOI:10.1242/jeb.145128


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[PMID]:28087632
[Au] Autor:Seleit A; Krämer I; Ambrosio E; Dross N; Engel U; Centanin L
[Ad] Endereço:Animal Physiology and Development, Centre for Organismal Studies (COS) Heidelberg, Im Neuenheimer Feld 230, Heidelberg 69120, Germany.
[Ti] Título:Sequential organogenesis sets two parallel sensory lines in medaka.
[So] Source:Development;144(4):687-697, 2017 02 15.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Animal organs are typically formed during embryogenesis by following one specific developmental programme. Here, we report that neuromast organs are generated by two distinct and sequential programmes that result in parallel sensory lines in medaka embryos. A ventral posterior lateral line (pLL) is composed of neuromasts deposited by collectively migrating cells whereas a midline pLL is formed by individually migrating cells. Despite the variable number of neuromasts among embryos, the sequential programmes that we describe here fix an invariable ratio between ventral and midline neuromasts. Mechanistically, we show that the formation of both types of neuromasts depends on the chemokine receptor genes and , illustrating how common molecules can mediate different morphogenetic processes. Altogether, we reveal a self-organising feature of the lateral line system that ensures a proper distribution of sensory organs along the body axis.
[Mh] Termos MeSH primário: Mutação
Organogênese
Oryzias/embriologia
Oryzias/fisiologia
[Mh] Termos MeSH secundário: Animais
Padronização Corporal
Movimento Celular
Quimiocinas/metabolismo
Proteínas de Fluorescência Verde/metabolismo
Hibridização In Situ
Sistema da Linha Lateral
Mecanorreceptores/metabolismo
Receptores CXCR/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Chemokines); 0 (Receptors, CXCR); 147336-22-9 (Green Fluorescent Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170115
[St] Status:MEDLINE
[do] DOI:10.1242/dev.142752


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[PMID]:27965055
[Au] Autor:Nogare DD; Nikaido M; Somers K; Head J; Piotrowski T; Chitnis AB
[Ad] Endereço:Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, United States. Electronic address: dalledam@mail.nih.gov.
[Ti] Título:In toto imaging of the migrating Zebrafish lateral line primordium at single cell resolution.
[So] Source:Dev Biol;422(1):14-23, 2017 02 01.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The zebrafish Posterior Lateral Line primordium (PLLp) has emerged as an important model system for studying many aspects of development, including cell migration, cell type specification and tissue morphogenesis. Despite this, basic aspects of PLLp biology remain incompletely understood. The PLLp is a group of approximately 140 cells which pioneers the formation of the Posterior Lateral Line (LL) system by migrating along the length of the embryo, periodically depositing clusters of epithelial cells, which will go on to form the mature sense organs of the lateral line, called neuromasts. The neuromasts are formed within the migrating PLLp as protoneuromasts: the first protoneuromast is formed close to the trailing end and additional protoneuromasts are formed sequentially, progressively closer to the leading edge of the migrating collective. We imaged the migration of PLL primordia and tracked every cell in the lateral line system over the course of migration. From this data set we unambiguously determined the lineage and fate of every cell deposited by the migrating PLLp. We show that, on average, proliferation across the entire PLLp is weakly patterned, with leading cells tending to divide more slowly than trailing cells. Neuromasts are formed sequentially by local expansion of existing cells along the length of the PLLp, not by self-renewing stem cell-like divisions of a restricted leading population that is highly proliferative. The fate of deposited cells, either within neuromasts or as interneuromast cells (in between deposited neuromasts) is not determined by any obvious stereotyped lineages. Instead, it is determined somewhat stochasitcailly, as a function of a cells distance from the center of a maturing protoneuromast. Together, our data provide a rigorous baseline for the behavior of the PLLp, which can be used to inform further study of this important model system.
[Mh] Termos MeSH primário: Sistema da Linha Lateral/embriologia
Peixe-Zebra/embriologia
[Mh] Termos MeSH secundário: Animais
Ciclo Celular
Divisão Celular
Movimento Celular
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, N.I.H., INTRAMURAL
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171120
[Lr] Data última revisão:
171120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161215
[St] Status:MEDLINE


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[PMID]:27847309
[Au] Autor:Stengel D; Zindler F; Braunbeck T
[Ad] Endereço:Aquatic Ecology and Toxicology Group, Center for Organismal Studies (COS), University of Heidelberg, Im Neuenheimer Feld 120, D-69120 Heidelberg, Germany.
[Ti] Título:An optimized method to assess ototoxic effects in the lateral line of zebrafish (Danio rerio) embryos.
[So] Source:Comp Biochem Physiol C Toxicol Pharmacol;193:18-29, 2017 Mar.
[Is] ISSN:1532-0456
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In order to clarify the suitability of the lateral line of zebrafish (Danio rerio) embryos as a model for the screening of ototoxic (neurotoxic) effects, existing neuromast assays were adapted, improved and validated with a series of chemicals known or unknown for their ototoxic potential (caffeine copper sulfate, dichlorvos, 2.4-dinitrotoluene, neomycin, 4-nonylphenol, perfluorooctanesulfonic acid). Present methods were improved by (1) the introduction of a 4-step scoring system, (2) the selection of neuromasts from both the anterior and posterior lateral line systems, (3) a combined DASPEI/DAPI staining applied after both a continuous and pulse exposure scenario, and (4) an additional screening for nuclear fragmentation. Acute toxicities of the model substances were determined by means of the fish embryo test as specified in OECD TG 236, and EC concentrations were used as the highest test concentration in the neuromast assay. The enhanced neuromast assay identified known ototoxic substances such as neomycin and copper sulfate as ototoxic at sensitivities similar to those of established methods, with pulse exposure leading to stronger effects than continuous exposure. Except for caffeine, all substances tested (dichlorvos, 2.4-dinitrotoluene, 4-nonylphenol, perfluorooctanesulfonic acid) produced significant toxic effects in neuromasts at EC concentrations. Depending on the test substances and their location along the lateral line, specific neuromasts differed in sensitivity. Generally, neuromasts proved more sensitive in the pulse exposure scenario. Whereas for neomycin and copper sulfate neuromasts located along the anterior lateral line were more sensitive, posterior lateral line neuromasts proved more sensitive for the other test substances. Nuclear fragmentation could not only be associated with all test substances, but, albeit at lower frequencies, also with negative controls, and could, therefore, not be assigned specifically to chemical damage. The study thus documented that for a comprehensive evaluation of lateral line damage both neuromasts from the anterior and the posterior lateral line have to be considered. Given the apparently rapid regeneration of hair cells, pulse exposure seems more appropriate for the identification of lateral line neurotoxicity than continuous exposure.
[Mh] Termos MeSH primário: Embrião não Mamífero/efeitos dos fármacos
Sistema da Linha Lateral/efeitos dos fármacos
Neurotoxinas/toxicidade
Peixe-Zebra/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Animais
Sulfato de Cobre/toxicidade
Células Ciliadas Auditivas/efeitos dos fármacos
Síndromes Neurotóxicas
Fenóis/toxicidade
Regeneração/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Neurotoxins); 0 (Phenols); I03GBV4WEL (4-nonylphenol); LRX7AJ16DT (Copper Sulfate)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170224
[Lr] Data última revisão:
170224
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161117
[St] Status:MEDLINE



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BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde