Base de dados : MEDLINE
Pesquisa : A13.655 [Categoria DeCS]
Referências encontradas : 65 [refinar]
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[PMID]:28250103
[Au] Autor:Park S; Piriatinskiy G; Zeevi D; Ben-David J; Yossifon G; Shavit U; Lotan T
[Ad] Endereço:Faculty of Mechanical Engineering, Technion, Haifa 32000, Israel.
[Ti] Título:The nematocyst's sting is driven by the tubule moving front.
[So] Source:J R Soc Interface;14(128), 2017 Mar.
[Is] ISSN:1742-5662
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The nematocyst is the explosive injection system of the phylum Cnidaria, and is one of the fastest delivery systems found in Nature. Exploring its injection mechanism is key for understanding predator-prey interactions and protection against jellyfish stinging. Here we analyse the injection of jellyfish nematocysts and ask how the build-up of the poly-γ-glutamate (pγGlu) osmotic potential inside the nematocyst drives its discharge. To control the osmotic potential, we used a two-channel microfluidic system to direct the elongating nematocyst tubule through oil, where no osmotic potential can develop, while keeping the nematocyst capsule in water at all times. In addition, the flow inside the tubule and the pγGlu concentration profiles were calculated by applying a one-dimensional mathematical model. We found that tubule elongation through oil is orders of magnitude slower than through water and that the injection rate of the nematocyst content is reduced. These results imply that the capsule's osmotic potential is not sufficient to drive the tubule beyond the initial stage. Our proposed model shows that the tubule is pulled by the high osmotic potential that develops at the tubule moving front. This new understanding is vital for future development of nematocyst-based systems such as osmotic nanotubes and transdermal drug delivery.
[Mh] Termos MeSH primário: Cnidários/fisiologia
Modelos Biológicos
Nematocisto/fisiologia
[Mh] Termos MeSH secundário: Animais
Cnidários/anatomia & histologia
Nematocisto/anatomia & histologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170303
[St] Status:MEDLINE


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[PMID]:27399728
[Au] Autor:Montgomery L; Seys J; Mees J
[Ad] Endereço:Flanders Marine Institute, InnovOcean Site, Wandelaarkaai 7, Ostende 8400, Belgium. loubudhram@gmail.com.
[Ti] Título:To Pee, or Not to Pee: A Review on Envenomation and Treatment in European Jellyfish Species.
[So] Source:Mar Drugs;14(7), 2016 Jul 08.
[Is] ISSN:1660-3397
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:There is a growing cause for concern on envenoming European species because of jellyfish blooms, climate change and globalization displacing species. Treatment of envenomation involves the prevention of further nematocyst release and relieving local and systemic symptoms. Many anecdotal treatments are available but species-specific first aid response is essential for effective treatment. However, species identification is difficult in most cases. There is evidence that oral analgesics, seawater, baking soda slurry and 42-45 °C hot water are effective against nematocyst inhibition and giving pain relief. The application of topical vinegar for 30 s is effective on stings of specific species. Treatments, which produce osmotic or pressure changes can exacerbate the initial sting and aggravate symptoms, common among many anecdotal treatments. Most available therapies are based on weak evidence and thus it is strongly recommended that randomized clinical trials are undertaken. We recommend a vital increase in directed research on the effect of environmental factors on envenoming mechanisms and to establish a species-specific treatment. Adequate signage on jellyfish stings and standardized first aid protocols with emphasis on protective equipment and avoidance of jellyfish to minimize cases should be implemented in areas at risk.
[Mh] Termos MeSH primário: Cifozoários/efeitos dos fármacos
Cifozoários/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Animais
Mordeduras e Picadas
Mudança Climática
Meio Ambiente
Europa (Continente)
Nematocisto/efeitos dos fármacos
Nematocisto/crescimento & desenvolvimento
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170510
[Lr] Data última revisão:
170510
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160712
[St] Status:MEDLINE


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[PMID]:26827260
[Au] Autor:DeClerck MP; Bailey Y; Craig D; Lin M; Auerbach LJ; Linney O; Morrison DE; Patry W; Auerbach PS
[Ad] Endereço:Division of Emergency Medicine, Department of Surgery, Stanford University School of Medicine, Stanford, CA (Drs CeClerck, Bailey, Craig, and P. Auerbach; Ms Lin; and Ms L. Auerbach). Electronic address: mpdeclerck@gmail.com.
[Ti] Título:Efficacy of Topical Treatments for Chrysaora chinensis Species: A Human Model in Comparison with an In Vitro Model.
[So] Source:Wilderness Environ Med;27(1):25-38, 2016 Mar.
[Is] ISSN:1545-1534
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: This study sought to create a model for testing topical treatment of jellyfish stings. It sought to determine which treatments 1) stimulate/inhibit nematocyst discharge; 2) decrease pain; and 3) decrease skin inflammation; it also sought to discover whether there is a clinical correlation between stimulated nematocyst discharge observed in vitro to the pain and erythema experienced by humans stung by a particular species of jellyfish, C chinensis. METHODS: Chrysaora chinensis stung 96 human subjects, who were then treated with isopropyl alcohol, hot water, acetic acid, papain meat tenderizer, lidocaine, or sodium bicarbonate. Pain and erythema were measured. In a separate experiment, nematocysts were examined microscopically after exposure to the same topical treatments used in the human experiment. RESULTS: Forearms treated with papain showed decreased mean pain over the first 30 minutes after being stung, relative to placebo, although only by a small amount. The other topical treatments tested did not reach statistical significance. Sodium bicarbonate may reduce erythema after 30 minutes of treatment; sodium bicarbonate and papain may reduce erythema at 60 minutes. The other topical treatments tested did not reach statistical significance. Nematocyst discharge in vitro occurred when tentacles of C chinensis were exposed to acetic acid or isopropyl alcohol. Sodium bicarbonate, papain, heated water, and lidocaine did not induce nematocyst discharge. CONCLUSIONS: Papain-containing meat tenderizer used as a topical treatment for C chinensis stings may decrease pain. Although there is published experimental support for the concept that in vitro nematocyst discharge correlates with in vivo human pain perception, no definitive randomized controlled trial, including ours, has yet provided incontrovertible evidence of this assertion. Despite this study's limitations, it presents a viable basis for future human studies looking at the efficacy of topical treatments for jellyfish stings.
[Mh] Termos MeSH primário: Mordeduras e Picadas/terapia
Inflamação/terapia
Nematocisto/fisiologia
Manejo da Dor/métodos
Cifozoários/fisiologia
[Mh] Termos MeSH secundário: Administração Cutânea
Adolescente
Adulto
Idoso
Animais
Feminino
Seres Humanos
Masculino
Meia-Idade
Pele/patologia
Especificidade da Espécie
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170110
[Lr] Data última revisão:
170110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160201
[St] Status:MEDLINE


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[PMID]:26825338
[Au] Autor:Thangaraj S; Bragadeeswaran S; Srikumaran N; Suguna A
[Ti] Título:Analgesic and CNS Depressant Activities of Sea Anemone Heteractis aurora Nematocyst Toxin.
[So] Source:Cent Nerv Syst Agents Med Chem;16(3):167-172, 2016.
[Is] ISSN:1875-6166
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Marine organisms are the excellent sources for biologically active compounds. Cnidarian venoms are potentially valuable materials used for biomedical research and drug development. The present work was carried out to analyse haemolytic, analgesic and CNS depressant activity of sea anemone Heteractis aurora. In haemolytic assay, among the five different RBC blood cells, the chicken blood exhibited maximum hemolytic activity of 64 Hemolytic Unit (HU). The maximum Analgesic Ratio (AR) of 5 recorded at 15 and 30 min interval and minimum was recorded after 45, 60 and 120 min time intervals. In jumping response activity, the maximum of 5 AR recorded at 15, 30 & 45 min and minimum was recorded at 90 & 120 min time intervals. The maximum decrease of depressant activity of 45.07% was determined in CNS depressant activity. Anti-inflammatory activity showed significant inhibition by crude extract of Heteractis aurora.
[Mh] Termos MeSH primário: Analgésicos/uso terapêutico
Depressores do Sistema Nervoso Central/uso terapêutico
Venenos de Cnidários/uso terapêutico
Nematocisto
Anêmonas-do-Mar
[Mh] Termos MeSH secundário: Analgésicos/isolamento & purificação
Analgésicos/farmacologia
Animais
Bovinos
Depressores do Sistema Nervoso Central/isolamento & purificação
Depressores do Sistema Nervoso Central/farmacologia
Galinhas
Venenos de Cnidários/isolamento & purificação
Venenos de Cnidários/farmacologia
Edema/tratamento farmacológico
Edema/metabolismo
Cabras
Hemólise/efeitos dos fármacos
Hemólise/fisiologia
Seres Humanos
Masculino
Camundongos
Medição da Dor/efeitos dos fármacos
Medição da Dor/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Analgesics); 0 (Central Nervous System Depressants); 0 (Cnidarian Venoms)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170223
[Lr] Data última revisão:
170223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160131
[St] Status:MEDLINE


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[PMID]:26761033
[Au] Autor:Yanagihara AA; Wilcox C; King R; Hurwitz K; Castelfranco AM
[Ad] Endereço:Department of Tropical Medicine, Medical Microbiology and Pharmacology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI 96822, USA. ayanagih@hawaii.edu.
[Ti] Título:Experimental Assays to Assess the Efficacy of Vinegar and Other Topical First-Aid Approaches on Cubozoan (Alatina alata) Tentacle Firing and Venom Toxicity.
[So] Source:Toxins (Basel);8(1), 2016 Jan 11.
[Is] ISSN:2072-6651
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Despite the medical urgency presented by cubozoan envenomations, ineffective and contradictory first-aid management recommendations persist. A critical barrier to progress has been the lack of readily available and reproducible envenomation assays that (1) recapitulate live-tentacle stings; (2) allow quantitation and imaging of cnidae discharge; (3) allow primary quantitation of venom toxicity; and (4) employ rigorous controls. We report the implementation of an integrated array of three experimental approaches designed to meet the above-stated criteria. Mechanistically overlapping, yet distinct, the three approaches comprised (1) direct application of test solutions on live tentacles (termed tentacle solution assay, or TSA) with single image- and video-microscopy; (2) spontaneous stinging assay using freshly excised tentacles overlaid on substrate of live human red blood cells suspended in agarose (tentacle blood agarose assays, or TBAA); and (3) a "skin" covered adaptation of TBAA (tentacle skin blood agarose assay, or TSBAA). We report the use and results of these assays to evaluate the efficacy of topical first-aid approaches to inhibit tentacle firing and venom activity. TSA results included the potent stimulation of massive cnidae discharge by alcohols but only moderate induction by urine, freshwater, and "cola" (carbonated soft drink). Although vinegar, the 40-year field standard of first aid for the removal of adherent tentacles, completely inhibited cnidae firing in TSA and TSBAA ex vivo models, the most striking inhibition of both tentacle firing and subsequent venom-induced hemolysis was observed using newly-developed proprietary formulations (Sting No More™) containing copper gluconate, magnesium sulfate, and urea.
[Mh] Termos MeSH primário: Ácido Acético/uso terapêutico
Mordeduras e Picadas/tratamento farmacológico
Venenos de Cnidários/toxicidade
Cubomedusas
[Mh] Termos MeSH secundário: Ácido Acético/farmacologia
Administração Tópica
Animais
Bioensaio
Eritrócitos/efeitos dos fármacos
Primeiros Socorros
Hemólise/efeitos dos fármacos
Seres Humanos
Nematocisto/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cnidarian Venoms); Q40Q9N063P (Acetic Acid)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:170805
[Lr] Data última revisão:
170805
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160114
[St] Status:MEDLINE


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[PMID]:26718259
[Au] Autor:Qu X; Fan L; Zhong T; Li G; Xia X; Long H; Huang D; Shu W
[Ad] Endereço:Engineering Lab of Endangered Medicinal Resources in Southwest China, Medicinal Plants Garden of Guangxi Zhuang Autonomous Region, 530023, China. Electronic address: 239653129@qq.com.
[Ti] Título:The nematocysts venom of Chrysaora helvola Brandt leads to apoptosis-like cell death accompanied by uncoupling of oxidative phosphorylation.
[So] Source:Toxicon;110:74-8, 2016 Feb.
[Is] ISSN:1879-3150
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The present work investigated the effects of the nematocysts venom (NV) from the Chrysaora helvola Brandt (C. helvola) jellyfish on the human nasopharyngeal carcinoma cell line, CNE-2. The medium lethal concentration (LC50), quantified by MTT assays, was 1.7 ± 0.53 µg/mL (n = 5). An atypical apoptosis-like cell death was confirmed by LDH release assay and Annexin V-FITC/PI staining-based flow cytometry. Interestingly, activation of caspase-4 other than caspase-3, -8, -9 and -1 was observed. Moreover, the NV stimuli caused a time-dependent loss of mitochondrial membrane potential (ΔΨm) as was an intracellular ROS burst. These results indicated that there was uncoupling of oxidative phosphorylation (UOP). An examination of the intracellular pH value by a pH-sensitive fluorescent probe, BCECF, suggested that the UOP was due to the time-dependent increase in the intracellular pH. This is the first report that jellyfish venom can induce UOP.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Venenos de Cnidários/farmacologia
Descoberta de Drogas
Neoplasias Nasofaríngeas/tratamento farmacológico
Fosforilação Oxidativa/efeitos dos fármacos
Cifozoários/química
Desacopladores/farmacologia
[Mh] Termos MeSH secundário: Animais
Antineoplásicos/isolamento & purificação
Apoptose/efeitos dos fármacos
Carcinoma/tratamento farmacológico
Carcinoma/metabolismo
Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
China
Venenos de Cnidários/isolamento & purificação
Seres Humanos
Concentração de Íons de Hidrogênio
Cinética
Dose Letal Mediana
Potencial da Membrana Mitocondrial/efeitos dos fármacos
Neoplasias Nasofaríngeas/metabolismo
Nematocisto/química
Nematocisto/crescimento & desenvolvimento
Oceano Pacífico
Espécies Reativas de Oxigênio/agonistas
Espécies Reativas de Oxigênio/metabolismo
Mucosa Respiratória/efeitos dos fármacos
Cifozoários/crescimento & desenvolvimento
Desacopladores/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Cnidarian Venoms); 0 (Reactive Oxygen Species); 0 (Uncoupling Agents); 0 (sea nettle toxin)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160101
[St] Status:MEDLINE


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[PMID]:26511919
[Au] Autor:Gülsahin N
[Ad] Endereço:Mugla Sitki Koçman University Faculty of Fisheries, Department of Hydrobiology, Mugla, Turkey.
[Ti] Título:Preliminary Study on Nematocyst Types and Venom Isolation of Cassiopea andromeda Forskål, 1775 (Scyphozoa, Cnidaria) from Turkey.
[So] Source:Cent Nerv Syst Agents Med Chem;16(3):208-212, 2016.
[Is] ISSN:1875-6166
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Nematocyst types of Cassiopea andromeda were investigated. Medusae samples were taken from Güllük Bay, Mugla, Turkey. Nematocyst samples from oral arms of C. andromeda were observed on light microscope and photographed. Birhopaloid and a-isorhiza nematocyst types were found in C. andromeda. Moreover, it was seen that nematocyst sizes increased with increasing the bell diameters of the individuals. Also, the venom of the species was isolated and injected intramuscularly to Cyprinus carpio juveniles. Signs of partial paralysis, raking, and immobilized fins were observed in the juveniles consequently. Death was observed for the fishes which were 3-4 g in the range of weight. This study is a preliminary work on nematocysts and venom of C. andromeda. Further studies on neurotoxic effects of nematocyst venoms of this species should follow.
[Mh] Termos MeSH primário: Venenos de Cnidários/isolamento & purificação
Venenos de Cnidários/toxicidade
Nematocisto
[Mh] Termos MeSH secundário: Animais
Baías
Carpas
Venenos de Cnidários/administração & dosagem
Injeções Intramusculares
Músculo Esquelético/efeitos dos fármacos
Músculo Esquelético/patologia
Turquia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cnidarian Venoms)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170223
[Lr] Data última revisão:
170223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151030
[St] Status:MEDLINE


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[PMID]:26464356
[Au] Autor:Gacesa R; Chung R; Dunn SR; Weston AJ; Jaimes-Becerra A; Marques AC; Morandini AC; Hranueli D; Starcevic A; Ward M; Long PF
[Ad] Endereço:Institute of Pharmaceutical Science, King's College London, 150 Stamford Street, London, SE1 9NH, UK.
[Ti] Título:Gene duplications are extensive and contribute significantly to the toxic proteome of nematocysts isolated from Acropora digitifera (Cnidaria: Anthozoa: Scleractinia).
[So] Source:BMC Genomics;16:774, 2015 Oct 13.
[Is] ISSN:1471-2164
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Gene duplication followed by adaptive selection is a well-accepted process leading to toxin diversification in venoms. However, emergent genomic, transcriptomic and proteomic evidence now challenges this role to be at best equivocal to other processess . Cnidaria are arguably the most ancient phylum of the extant metazoa that are venomous and such provide a definitive ancestral anchor to examine the evolution of this trait. METHODS: Here we compare predicted toxins from the translated genome of the coral Acropora digitifera to putative toxins revealed by proteomic analysis of soluble proteins discharged from nematocysts, to determine the extent to which gene duplications contribute to venom innovation in this reef-building coral species. A new bioinformatics tool called HHCompare was developed to detect potential gene duplications in the genomic data, which is made freely available ( https://github.com/rgacesa/HHCompare ). RESULTS: A total of 55 potential toxin encoding genes could be predicted from the A. digitifera genome, of which 36 (65 %) had likely arisen by gene duplication as evinced using the HHCompare tool and verified using two standard phylogeny methods. Surprisingly, only 22 % (12/55) of the potential toxin repertoire could be detected following rigorous proteomic analysis, for which only half (6/12) of the toxin proteome could be accounted for as peptides encoded by the gene duplicates. Biological activities of these toxins are dominatedby putative phospholipases and toxic peptidases. CONCLUSIONS: Gene expansions in A. digitifera venom are the most extensive yet described in any venomous animal, and gene duplication plays a significant role leading to toxin diversification in this coral species. Since such low numbers of toxins were detected in the proteome, it is unlikely that the venom is evolving rapidly by prey-driven positive natural selection. Rather we contend that the venom has a defensive role deterring predation or harm from interspecific competition and overgrowth by fouling organisms. Factors influencing translation of toxin encoding genes perhaps warrants more profound experimental consideration.
[Mh] Termos MeSH primário: Antozoários/genética
Evolução Molecular
Duplicação Gênica
Proteoma/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Antozoários/patogenicidade
Venenos de Cnidários/genética
Venenos de Cnidários/toxicidade
Genoma
Nematocisto/metabolismo
Filogenia
Proteoma/toxicidade
Seleção Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cnidarian Venoms); 0 (Proteome)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151015
[St] Status:MEDLINE
[do] DOI:10.1186/s12864-015-1976-4


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[PMID]:26309256
[Au] Autor:Kitatani R; Yamada M; Kamio M; Nagai H
[Ad] Endereço:Department of Ocean Sciences, Tokyo University of Marine Science and Technology, Minato-ku, Tokyo, Japan.
[Ti] Título:Length Is Associated with Pain: Jellyfish with Painful Sting Have Longer Nematocyst Tubules than Harmless Jellyfish.
[So] Source:PLoS One;10(8):e0135015, 2015.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A large number of humans are stung by jellyfish all over the world. The stings cause acute pain followed by persistent pain and local inflammation. Harmful jellyfish species typically cause strong pain, whereas harmless jellyfish cause subtle or no pain. Jellyfish sting humans by injecting a tubule, contained in the nematocyst, the stinging organ of jellyfish. The tubule penetrates into the skin leading to venom injection. The detailed morphology of the nematocyst tubule and molecular structure of the venom in the nematocyst has been reported; however, the mechanism responsible for the difference in pain that is caused by harmful and harmless jellyfish sting has not yet been explored or explained. Therefore, we hypothesized that differences in the length of the nematocyst tubule leads to different degrees of epithelial damage. The initial acute pain might be generated by penetration of the tubule, which stimulates pain receptor neurons, whilst persistent pain might be caused by injection of venom into the epithelium. To test this hypothesis we compared the lengths of discharged nematocyst tubules from harmful and harmless jellyfish species and evaluated their ability to penetrate human skin. The results showed that the harmful jellyfish species, Chrysaora pacifica, Carybdea brevipedalia, and Chironex yamaguchii, causing moderate to severe pain, have nematocyst tubules longer than 200 µm, compared with a jellyfish species that cause little or no pain, Aurelia aurita. The majority of the tubules of harmful jellyfishes, C. yamaguchii and C. brevipedalia, were sufficiently long to penetrate the human epidermis and physically stimulate the free nerve endings of Aδ pain receptor fibers around plexuses to cause acute pain and inject the venom into the human skin epithelium to cause persistent pain and inflammation.
[Mh] Termos MeSH primário: Mordeduras e Picadas/complicações
Nematocisto/anatomia & histologia
Dor/complicações
Cifozoários/anatomia & histologia
[Mh] Termos MeSH secundário: Animais
Mordeduras e Picadas/induzido quimicamente
Venenos de Cnidários/toxicidade
Nociceptores/efeitos dos fármacos
Cifozoários/química
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cnidarian Venoms)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:150902
[Lr] Data última revisão:
150902
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150827
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0135015


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[PMID]:26201970
[Au] Autor:Horiike T; Nagai H; Kitani S
[Ad] Endereço:Department of Ocean Sciences, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Tokyo, Japan.
[Ti] Título:Identification of allergens in the box jellyfish Chironex yamaguchii that cause sting dermatitis.
[So] Source:Int Arch Allergy Immunol;167(2):73-82, 2015.
[Is] ISSN:1423-0097
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Jellyfish stings cause painful, papular-urticarial eruptions due to the immediate allergic, acute toxic and persistent inflammatory responses. In spite of many marine accidents and their economic impact, modes of first-aid treatment remain conventional and specific allergen and medical treatment are not yet available. The purpose of this study was to define the specific allergen of the box jellyfish Chironex yamaguchii and to study the precise mechanism of the resulting dermatitis. METHODS: We comprehensively studied the immunoglobulin-binding molecules from the box jellyfish C. yamaguchii with a purification procedure and Western blotting, using sera from 1 patient and from several controls. RESULTS: From the nematocyst wall and spine, we detected IgG-binding acidic glycoprotein (of 66 and 30 kDa) as determined by Western blot and ion-exchange chromatography. In addition, the 66-kDa protein was found to be an asparagine residue-coupled N-linked glycoprotein and the epitope resided in the protein fraction. We found that CqTX-A, the major toxic protein of the nematocyst, is also a heat-stable IgE-binding allergen. This was confirmed as a 45-kDa protein by Western blot from both nematocyst extracts and purified CqTX-A. CONCLUSIONS: The detection of these proteins may, in part, explain the combined immediate allergic-toxic and persistent allergic responses. Hopefully, our findings will lead to the development of specific venom immunotherapy for marine professional workers and tourists for jellyfish-sting dermatitis and anaphylaxis.
[Mh] Termos MeSH primário: Alérgenos/isolamento & purificação
Mordeduras e Picadas/etiologia
Venenos de Cnidários/isolamento & purificação
Cubomedusas/imunologia
Cubomedusas/patogenicidade
Dermatite/etiologia
[Mh] Termos MeSH secundário: Adulto
Alérgenos/toxicidade
Animais
Reações Antígeno-Anticorpo
Western Blotting
Venenos de Cnidários/imunologia
Venenos de Cnidários/toxicidade
Glicosilação
Seres Humanos
Imunoglobulina E/metabolismo
Imunoglobulina G/metabolismo
Masculino
Nematocisto/química
Nematocisto/imunologia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Allergens); 0 (Chiropsalmus quadrigatus toxin-A); 0 (Cnidarian Venoms); 0 (Immunoglobulin G); 37341-29-0 (Immunoglobulin E)
[Em] Mês de entrada:1511
[Cu] Atualização por classe:150904
[Lr] Data última revisão:
150904
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150724
[St] Status:MEDLINE
[do] DOI:10.1159/000434721



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