Base de dados : MEDLINE
Pesquisa : A13.706 [Categoria DeCS]
Referências encontradas : 3597 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 360 ir para página                         

  1 / 3597 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29281706
[Au] Autor:Shen M; Qu L; Ma M; Dou T; Lu J; Guo J; Hu Y; Wang X; Li Y; Wang K; Yang N
[Ad] Endereço:Jiangsu Institute of Poultry Science, Chinese Academy of Agricultural Science, Yangzhou, China.
[Ti] Título:A genome-wide study to identify genes responsible for oviduct development in chickens.
[So] Source:PLoS One;12(12):e0189955, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Molecular genetic tools provide a method for improving the breeding selection of chickens (Gallus gallus). Although some studies have identified genes affecting egg quality, little is known about the genes responsible for oviduct development. To address this issue, here we used a genome-wide association (GWA) study to detect genes or genomic regions that are related to oviduct development in a chicken F2 resource population by employing high-density 600 K single-nucleotide polymorphism (SNP) arrays. For oviduct length and weight, which exhibited moderate heritability estimates of 0.35 and 0.39, respectively, chromosome 1 (GGA1) explained 9.45% of the genetic variance, while GGA4 to GGA8 and GGA11 explained over 1% of the variance. Independent univariate genome-wide screens for oviduct length and weight detected 69 significant SNPs on GGA1 and 49 suggestive SNPs on GGA1, GGA4, and GGA8. One hundred and fourteen suggestive SNPs were associated with oviduct length, while 73 SNPs were associated with oviduct weight. The significant genomic regions affecting oviduct weight ranged from 167.79-174.29 Mb on GGA1, 73.16-75.70 Mb on GGA4, and 4.88-4.92 Mb on GGA8. The genes CKAP2, CCKAR, NCAPG, IGFBP3, and GORAB were shown to have potential roles in oviduct development. These genes are involved in cell survival, appetite, and growth control. Our results represent the first GWA analysis of genes controlling oviduct weight and length. The identification of genomic loci and potential candidate genes affecting oviduct development greatly increase our understanding of the genetic basis underlying oviduct development, which could have an impact on the selection of egg quality.
[Mh] Termos MeSH primário: Estudo de Associação Genômica Ampla
Oviductos/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Animais
Galinhas
Feminino
Variação Genética
Desequilíbrio de Ligação
Polimorfismo de Nucleotídeo Único
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171228
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189955


  2 / 3597 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29219807
[Au] Autor:Qi X; Zhang H; Xue T; Yang B; Deng M; Wang J
[Ad] Endereço:1​College of Veterinary Medicine, Northwest A&F University, Yangling 712100, PR China.
[Ti] Título:Down-regulation of cellular protein heme oxygenase-1 inhibits proliferation of avian influenza virus H9N2 in chicken oviduct epithelial cells.
[So] Source:J Gen Virol;99(1):36-43, 2018 Jan.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The pathogenesis of H9N2 subtype avian influenza virus (AIV) infection in hens is often related to oviduct tissue damage. Our previous study suggested that H9N2 AIV induces cellular apoptosis by activating reactive oxygen species (ROS) accumulation and mitochondria-mediated apoptotic signalling in chicken oviduct epithelial cells (COECs). Heme oxygenase-1 (HO-1) is an inducible enzyme that exerts protective effects against oxidative stress and activated HO-1 was recently shown to have antiviral activity. To study the potential involvement of HO-1 in H9N2 AIV proliferation, the role of its expression in H9N2-infected COECs was further investigated. Our results revealed that H9N2 AIV infection significantly up-regulated the expression of HO-1 and that HO-1 down-regulation by ZnPP, a classical inhibitor of HO-1, could inhibit H9N2 AIV replication in COECs. Similarly, the small interfering RNA (siRNA)-mediated knockdown of HO-1 also markedly decreased the virus production in H9N2-infected COECs. In contrast, adenoviral-mediated over-expression of HO-1 concomitantly promoted H9N2 AIV replication. Taken together, our study demonstrated the involvement of HO-1 in AIV H9N2 proliferation, and these findings suggested that HO-1 is a potential target for inhibition of AIV H9N2 replication.
[Mh] Termos MeSH primário: Proteínas Aviárias/genética
Inibidores Enzimáticos/farmacologia
Células Epiteliais/efeitos dos fármacos
Heme Oxigenase-1/genética
Protoporfirinas/farmacologia
Espécies Reativas de Oxigênio/antagonistas & inibidores
[Mh] Termos MeSH secundário: Adenoviridae/genética
Adenoviridae/metabolismo
Animais
Apoptose/efeitos dos fármacos
Proteínas Aviárias/agonistas
Proteínas Aviárias/antagonistas & inibidores
Proteínas Aviárias/metabolismo
Galinhas
Células Epiteliais/metabolismo
Células Epiteliais/virologia
Feminino
Regulação da Expressão Gênica
Vetores Genéticos/química
Vetores Genéticos/metabolismo
Heme Oxigenase-1/antagonistas & inibidores
Heme Oxigenase-1/metabolismo
Interações Hospedeiro-Patógeno/efeitos dos fármacos
Vírus da Influenza A Subtipo H9N2
Mitocôndrias/efeitos dos fármacos
Mitocôndrias/metabolismo
Oviductos/metabolismo
Oviductos/virologia
Estresse Oxidativo
Cultura Primária de Células
RNA Interferente Pequeno/genética
RNA Interferente Pequeno/metabolismo
Espécies Reativas de Oxigênio/agonistas
Espécies Reativas de Oxigênio/metabolismo
Replicação Viral/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Avian Proteins); 0 (Enzyme Inhibitors); 0 (Protoporphyrins); 0 (RNA, Small Interfering); 0 (Reactive Oxygen Species); 15442-64-5 (zinc protoporphyrin); EC 1.14.14.18 (Heme Oxygenase-1)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171229
[Lr] Data última revisão:
171229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171209
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000986


  3 / 3597 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28748255
[Au] Autor:Katsumata O; Mori M; Sawane Y; Niimura T; Ito A; Okamoto H; Fukaya M; Sakagami H
[Ad] Endereço:Department of Anatomy, Kitasato University School of Medicine, Sagamihara, Kanagawa, 252-0374, Japan.
[Ti] Título:Cellular and subcellular localization of ADP-ribosylation factor 6 in mouse peripheral tissues.
[So] Source:Histochem Cell Biol;148(6):577-596, 2017 Dec.
[Is] ISSN:1432-119X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:ADP-ribosylation factor 6 (Arf6) is a small GTPase that regulates endosomal trafficking and actin cytoskeleton remodeling. In the present study, we comprehensively examined the cellular and subcellular localization of Arf6 in adult mouse peripheral tissues by immunofluorescence and immunoelectron microscopy using the heat-induced antigen retrieval method with Tris-EDTA buffer (pH 9.0). Marked immunolabeling of Arf6 was observed particularly in epithelial cells of several tissues including the esophagus, stomach, small and large intestines, trachea, kidney, epididymis, oviduct, and uterus. In most epithelial cells of simple or pseudostratified epithelia, Arf6 exhibited predominant localization to the basolateral membrane and a subpopulation of endosomes. At an electron microscopic level, Arf6 was localized along the basolateral membrane, with dense accumulation at interdigitating processes and infoldings. Arf6 was present in a ring-like appearance at intercellular bridges in spermatogonia and spermatocytes in the testis and at the Flemming body of cytokinetic somatic cells in the ovarian follicle, thymus, and spleen. The present study provides anatomical clues to help understand the physiological roles of Arf6 at the whole animal level.
[Mh] Termos MeSH primário: Fatores de Ribosilação do ADP/análise
Epididimo/química
Intestino Delgado/química
Rim/química
Oviductos/química
Testículo/química
[Mh] Termos MeSH secundário: Animais
Reações Antígeno-Anticorpo
Feminino
Imunofluorescência
Células HeLa
Seres Humanos
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Endogâmicos ICR
Microscopia Imunoeletrônica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 3.6.5.2 (ADP-Ribosylation Factors); EC 3.6.5.2 (ADP-ribosylation factor 6)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171206
[Lr] Data última revisão:
171206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1007/s00418-017-1599-8


  4 / 3597 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28743800
[Au] Autor:Ghosh A; Syed SM; Tanwar PS
[Ad] Endereço:Gynaecology Oncology Group, School of Biomedical Sciences and Pharmacy, University of Newcastle, Callaghan, New South Wales, 2308, Australia.
[Ti] Título: genetic cell lineage tracing reveals that oviductal secretory cells self-renew and give rise to ciliated cells.
[So] Source:Development;144(17):3031-3041, 2017 09 01.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The epithelial lining of the fallopian tube is vital for fertility, providing nutrition to gametes and facilitating their transport. It is composed of two major cell types: secretory cells and ciliated cells. Interestingly, human ovarian cancer precursor lesions primarily consist of secretory cells. It is unclear why secretory cells are the dominant cell type in these lesions. Additionally, the underlying mechanisms governing fallopian tube epithelial homoeostasis are unknown. In the present study, we showed that across the different developmental stages of mouse oviduct, secretory cells are the most frequently dividing cells of the oviductal epithelium. genetic cell lineage tracing showed that secretory cells not only self-renew, but also give rise to ciliated cells. Analysis of a Wnt reporter mouse model and various Wnt target genes showed that the Wnt signaling pathway is involved in oviductal epithelial homoeostasis. By developing two triple-transgenic mouse models, we showed that Wnt/ß-catenin signaling is essential for self-renewal as well as the differentiation of secretory cells. In summary, our results provide mechanistic insight into oviductal epithelial homoeostasis.
[Mh] Termos MeSH primário: Linhagem da Célula
Autorrenovação Celular
Rastreamento de Células
Cílios/metabolismo
Oviductos/citologia
Oviductos/secreção
[Mh] Termos MeSH secundário: Animais
Diferenciação Celular
Proliferação Celular
Epitélio/patologia
Tubas Uterinas/patologia
Feminino
Predisposição Genética para Doença
Seres Humanos
Camundongos Endogâmicos C57BL
Neoplasias Ovarianas/patologia
Fator de Transcrição PAX8/metabolismo
Fatores de Risco
Via de Sinalização Wnt
beta Catenina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (PAX8 Transcription Factor); 0 (Pax8 protein, mouse); 0 (beta Catenin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.1242/dev.149989


  5 / 3597 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28973574
[Au] Autor:Guadalupe Rojas M; Grodowitz MJ; Reibenspies J; Reed DA; Perring TM; Allen ML
[Ad] Endereço:Biological Control of Pests Research Unit, USDA-ARS National Biological Control Laboratory, 59 Lee Road, Stoneville, MS 38776.
[Ti] Título:Allantoin Crystal Formation in Bagrada hilaris (Burmeister) (Heteroptera: Pentatomidae) Females.
[So] Source:J Insect Sci;17(3), 2017 May 01.
[Is] ISSN:1536-2442
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bagrada hilaris is a polyphagous herbivore reported as an invasive pest in the United States. During the course of dissecting Burmeister hilaris unique crystals were observed in both the midgut and oviducts. Crystals were identified using X-ray diffraction techniques. Both acicular (i.e., needle-like, slender, and/or tapered) and cubic (i.e., cube shaped) crystals were observed in six of 75 individuals examined (8.0%). The crystals were mainly observed in females (6.7%), followed by males (1.3%) with no crystals observed in the minimal number of nymphs examined (0%). Crystals of both types were detected in the midgut and lateral oviducts of the females and midgut in males. The acicular crystals often appeared as distinct bundles when present in the midgut and oviducts. Crystals varied in size with the acicular crystals ranging from 0.12 mm to 0.5 mm in length although the cubic crystals ranged in length from 0.25 mm to over 1.0 mm with widths of ∼0.25 mm. The cubic crystals were identified as allantoin although the acicular crystals were most likely dl-allantoin in combination with halite. While allantoin in a soluble form is often found in insect tissues and excreta; being present as a crystal, especially in such a large form, is curious and raises some interesting questions. More research is warranted to further understand mechanisms associated with such crystal formation in B. hilaris and can lead to a better understanding of the excretory process in this species and the role allantoin plays in the elimination of excess nitrogen.
[Mh] Termos MeSH primário: Alantoína/metabolismo
Heterópteros/metabolismo
[Mh] Termos MeSH secundário: Animais
Cristalização
Feminino
Trato Gastrointestinal/metabolismo
Masculino
Oviductos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
344S277G0Z (Allantoin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171025
[Lr] Data última revisão:
171025
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171004
[St] Status:MEDLINE
[do] DOI:10.1093/jisesa/iex051


  6 / 3597 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28797102
[Au] Autor:Zhang Y; Shao L; Li X; Zhong G
[Ad] Endereço:Department of Obstetrics and Gynecology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, PR China.
[Ti] Título:Uterotubal junction prevents chlamydial ascension via innate immunity.
[So] Source:PLoS One;12(8):e0183189, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ascension to the oviduct is necessary for Chlamydia to induce tubal infertility. Using the Chlamydia muridarum induction of hydrosalpinx mouse model, we have demonstrated a significant role of the uterotubal junction in preventing chlamydial ascending infection. First, delivery of C. muridarum to either side of the uterotubal junction resulted in significant reduction in live organisms from the tissues on the opposite sides. However, the recovery yields remained similar among different sections of the uterine horn. These observations suggest that the uterotubal junction may function as a barrier between the uterine horn and oviduct. Second, deficiency in innate immunity signaling pathways mediated by either MyD88 or STING significantly compromised the uterotubal junction barrier function, permitting C. muridarum to spread freely between uterine horn and oviduct. Finally, transcervical inoculation of C. muridarum led to significantly higher incidence of bilateral hydrosalpinges in the STING-deficient mice while the same inoculation mainly induced unilateral hydrosalpinx in the wild type mice, suggesting that the STING pathway-dependent uterotubal junction plays a significant role in preventing tubal pathology. Thus, we have demonstrated for the first time that the uterotubal junction is a functional barrier for preventing tubal infection by a sexually transmitted agent, providing the first in vivo evidence for detecting chlamydial infection by the STING pathway.
[Mh] Termos MeSH primário: Infecções por Chlamydia/patologia
Chlamydia muridarum/imunologia
Tubas Uterinas/patologia
Imunidade Inata
Oviductos/patologia
Infecções do Sistema Genital/patologia
Útero/patologia
[Mh] Termos MeSH secundário: Animais
Infecções por Chlamydia/imunologia
Infecções por Chlamydia/microbiologia
Modelos Animais de Doenças
Tubas Uterinas/imunologia
Tubas Uterinas/microbiologia
Feminino
Seres Humanos
Camundongos
Camundongos Endogâmicos C57BL
Oviductos/imunologia
Oviductos/microbiologia
Infecções do Sistema Genital/imunologia
Infecções do Sistema Genital/microbiologia
Salpingite/imunologia
Salpingite/microbiologia
Salpingite/patologia
Útero/imunologia
Útero/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170811
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183189


  7 / 3597 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28671963
[Au] Autor:Mattsson A; Brunström B
[Ad] Endereço:Department of Environmental Toxicology, Uppsala University, Uppsala, Sweden.
[Ti] Título:Effects of selective and combined activation of estrogen receptor α and ß on reproductive organ development and sexual behaviour in Japanese quail (Coturnix japonica).
[So] Source:PLoS One;12(7):e0180548, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Excess estrogen exposure of avian embryos perturbs reproductive organ development in both sexes and demasculinizes the reproductive behaviors of adult males. We have previously shown that these characteristic effects on the reproductive organs also can be induced by exposure of Japanese quail (Coturnix japonica) embryos to selective agonists of estrogen receptor alpha (ERα). In contrast, the male copulatory behavior is only weakly affected by developmental exposure to an ERα agonist. To further elucidate the respective roles of ERα and ERß in estrogen-induced disruption of sexual differentiation, we exposed Japanese quail embryos in ovo to the selective ERα agonist 16α-lactone-estradiol (16αLE2), the selective ERß agonist WAY-200070, or both substances in combination. The ERα agonist feminized the testes in male embryos and reduced cloacal gland size in adult males. Furthermore, anomalous retention and malformations of the Müllerian ducts/oviducts were seen in embryos and juveniles of both sexes. The ERß agonist did not induce any of these effects and did not influence the action of the ERα agonist. Male copulatory behavior was not affected by embryonic exposure to either the ERα- or the ERß-selective agonist but was slightly suppressed by treatment with the two compounds combined. Our results suggest that the reproductive organs become sexually differentiated consequent to activation of ERα by endogenous estrogens; excessive activation of ERα, but not ERß, during embryonic development may disrupt this process. Our results also suggest that the demasculinizing effect of estrogens on male copulatory behavior is only partly mediated by ERα and ERß, and may rather involve other estrogen-responsive pathways.
[Mh] Termos MeSH primário: Coturnix/fisiologia
Receptor alfa de Estrogênio/fisiologia
Receptor beta de Estrogênio/fisiologia
Oviductos/fisiologia
Comportamento Sexual Animal
Testículo/fisiologia
[Mh] Termos MeSH secundário: Animais
Receptor alfa de Estrogênio/agonistas
Receptor beta de Estrogênio/agonistas
Feminino
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Estrogen Receptor alpha); 0 (Estrogen Receptor beta)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170704
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0180548


  8 / 3597 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28394395
[Au] Autor:Kamimura T; Isobe N; Yoshimura Y
[Ad] Endereço:Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan.
[Ti] Título:Effects of inhibitors of transcription factors, nuclear factor-κB and activator protein 1, on the expression of proinflammatory cytokines and chemokines induced by stimulation with Toll-like receptor ligands in hen vaginal cells.
[So] Source:Poult Sci;96(3):723-730, 2017 Mar 01.
[Is] ISSN:1525-3171
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The goal of this study was to determine whether nuclear factor-κB (NFκB) and activator protein 1 (AP-1) were the responsible transcription factors for the induction of proinflammatory cytokines in hen vaginal cells stimulated by different Toll-like receptor (TLR) ligands. Cultured vaginal cells were treated with or without poly I:C (TLR3 ligand; dsRNA virus), lipopolysaccharide (LPS) (TLR4 ligand; gram-negative bacteria), flagellin (TLR5 ligand; bacterial flagellum), R848 (TLR7 ligand; ssRNA virus), and CpG-oligodeoxynucleotide (CpG-ODN) (TLR21 ligand; bacteria and DNA virus) in the presence or absence of different doses of BAY11-7085 (NFκB inhibitor) and tanshinone IIA (AP-1 inhibitor). Then, gene expressions of IL1B, IL6, and CXCLi2 were examined by real-time PCR analysis. The results showed that the induction of the expression of IL1B, IL6 and CXCLi2 by poly I:C, LPS, and CpG-ODN were suppressed by Bay11-7085, but not by tanshinone IIA. IL1B expression was upregulated by flagellin and R848, and the increase in its expression was suppressed by Bay11-7085, but not by tanshinone. These results suggest that NFκB is the responsible transcription factor for the expression of proinflammatory cytokines and chemokines, including I IL1B, IL6, and CXCLi2 in response to the ligands of TLR3, 4, and 21, and IL1B in response to the ligands of TLR5 and 7 in the vaginal cells.
[Mh] Termos MeSH primário: Galinhas/imunologia
Regulação da Expressão Gênica/efeitos dos fármacos
Imunossupressores/farmacologia
NF-kappa B/imunologia
Receptores Toll-Like/imunologia
Fator de Transcrição AP-1/imunologia
[Mh] Termos MeSH secundário: Animais
Proteínas Aviárias/genética
Proteínas Aviárias/imunologia
Células Cultivadas
Quimiocinas/metabolismo
Galinhas/genética
Citocinas/genética
Feminino
Ligantes
NF-kappa B/antagonistas & inibidores
Oviductos/microbiologia
Fator de Transcrição AP-1/antagonistas & inibidores
Vagina/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Avian Proteins); 0 (Chemokines); 0 (Cytokines); 0 (Immunosuppressive Agents); 0 (Ligands); 0 (NF-kappa B); 0 (Toll-Like Receptors); 0 (Transcription Factor AP-1)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170411
[St] Status:MEDLINE
[do] DOI:10.3382/ps/pew366


  9 / 3597 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28334862
[Au] Autor:Nathan A; Reinhardt P; Kruspe D; Jörß T; Groth M; Nolte H; Habenicht A; Herrmann J; Holschbach V; Toth B; Krüger M; Wang ZQ; Platzer M; Englert C
[Ad] Endereço:Molecular Genetics Lab.
[Ti] Título:The Wilms tumor protein Wt1 contributes to female fertility by regulating oviductal proteostasis.
[So] Source:Hum Mol Genet;26(9):1694-1705, 2017 May 01.
[Is] ISSN:1460-2083
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Although the zinc finger transcription factor Wt1 has been linked to female fertility, its precise role in this process has not yet been understood. We have sequenced the WT1 exons in a panel of patients with idiopathic infertility and have identified a missense mutation in WT1 in one patient out of eight. This mutation leads to an amino acid change within the zinc finger domain and results in reduced DNA binding. We utilized Wt1+/- mice as a model to mechanistically pinpoint the consequences of reduced Wt1 levels for female fertility. Our results indicate that subfertility in Wt1+/- female mice is a maternal effect caused by the Wt1-dependent de-regulation of Prss29, encoding a serine protease. Notably, blocking Prss29 activity was sufficient to rescue subfertility in Wt1+/- mice indicating Prss29 as a critical factor in female fertility. Molecularly, Wt1 represses expression of Prss29. De-repression and precocious expression of Prss29 in the oviduct of Wt1+/- mice interferes with pre-implantation development. Our study reveals a novel role for Wt1 in early mammalian development and identifies proteases as critical mediators of the maternal-embryonic interaction. Our data also suggest that the role of Wt1 in regulating fertility is conserved in mammals.
[Mh] Termos MeSH primário: Infertilidade Feminina/genética
Proteínas WT1/genética
Proteínas WT1/metabolismo
Tumor de Wilms/genética
Tumor de Wilms/metabolismo
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Sítios de Ligação
Proteínas de Ligação a DNA/genética
Modelos Animais de Doenças
Éxons
Feminino
Fertilidade/fisiologia
Seres Humanos
Infertilidade Feminina/sangue
Infertilidade Feminina/metabolismo
Camundongos
Camundongos Knockout
Mutação de Sentido Incorreto
Oviductos/metabolismo
Oviductos/patologia
Fatores de Transcrição/genética
Dedos de Zinco
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA-Binding Proteins); 0 (Transcription Factors); 0 (WT1 Proteins); 0 (WT1 protein, human)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170324
[St] Status:MEDLINE
[do] DOI:10.1093/hmg/ddx075


  10 / 3597 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28298331
[Au] Autor:Xi L; Liu Y; Tang Z; Sheng X; Zhang H; Weng Q; Xu M
[Ad] Endereço:College of Biological Sciences and Technology, Beijing Forestry University, Beijing, People's Republic of China; and.
[Ti] Título:Expression of leptin receptor in the oviduct of Chinese brown frog ( ).
[So] Source:Am J Physiol Regul Integr Comp Physiol;312(6):R912-R918, 2017 Jun 01.
[Is] ISSN:1522-1490
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The oviduct of Chinese brown frog ( ) expands specifically during prehibernation instead of in the breeding period. In this study, we investigated the expression of leptin receptor (Ob-Rb) in oviduct during the breeding period and prehibernation. Histologically, the oviduct of consists of glandular cells, tubule lumen, and epithelial cells. The oviductal weight and pipe diameter also revealed significant differences, which were higher in prehibernation than that of the breeding period. Ob-Rb was observed in stromal cells of oviductal tissue in both the breeding period and prehibernation. The mean protein and mRNA levels of the Ob-Rb were significantly higher in prehibernation as compared with the breeding period. In addition, oviductal content of leptin was also higher in prehibernation than that of the breeding period. These results suggested that oviduct of might be a target organ of leptin, and leptin may play an autocrine/paracrine role mediated by Ob-Rb in regulating the oviductal hypertrophy during prehibernation.
[Mh] Termos MeSH primário: Proteínas de Anfíbios/metabolismo
Oviductos/metabolismo
Ranidae/metabolismo
Receptores para Leptina/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Anfíbios/genética
Animais
Cruzamento
Feminino
Regulação da Expressão Gênica
Hibernação
Tamanho do Órgão
Oviductos/anatomia & histologia
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Ranidae/anatomia & histologia
Ranidae/genética
Receptores para Leptina/genética
Reprodução
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amphibian Proteins); 0 (RNA, Messenger); 0 (Receptors, Leptin)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170807
[Lr] Data última revisão:
170807
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170317
[St] Status:MEDLINE
[do] DOI:10.1152/ajpregu.00020.2017



página 1 de 360 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde